• α-glucosidase is an essential enzyme for the production of isomaltooligosaccharides (IMOs). (mdpi.com)
  • However, one-pot icaritin production required reported enzymes to be immobilized or bifunctional enzymes to hydrolyze substrate with long reaction time, which caused complicated operations and high costs. (biomedcentral.com)
  • For food protein hydrolysates, the substrate and enzymes are mixed in huge, tempered tanks, and require continuous stirring, as well as a constant temperature and pH level for several hours. (springeropen.com)
  • It was also around this time that researchers began immobilizing the enzymes onto a solid substrate, allowing them to be recycled and reused without the high costs involved in the isolation and purification of large quantities of enzymes. (news-medical.net)
  • 2012 ). When using enzymes for digesting food proteins, an enzyme specific peptide composition, the so-called peptide fingerprint is formed. (springeropen.com)
  • One potential application of such enzymes is in the treatment of food waste, which is rich in carbohydrates, fats, and proteins that could potentially be recycled into other products such as biofuels. (news-medical.net)
  • This enzyme is a natural lactonase that is able to hydrolyze acyl-homoserine lactones (AHL) that are involved in the quorum sensing (QS) of Gram-negative bacteria (e.g. (nature.com)
  • Enzymes are used to hydrolyze lactose into glucose, which can then be used in other food manufacturing processes. (news-medical.net)
  • Hence, within the frame of this thesis, the use of eco-technologies such as cold remote plasma, a bio-compatible conductive (PEDOT:PSS) polymer coating, and a bio-based crosslinker "genipin" which has low toxicity, to immobilize glucose oxidase (GOx) enzyme on conductive carbon fiber-based nonwoven textiles was investigated. (diva-portal.org)
  • Bacterial secreted materials from both Gram-negative and positive bacteria were harmless on Sso Pox-W263I activity and could reactivate heat-inactivated enzyme. (nature.com)
  • Bacteria and cells release digestive enzymes or their inactive precursors. (springeropen.com)
  • . As researchers began to unravel the mechanisms behind the observed activity of enzymes a wider range of uses were found, one prominent example being the discovery of penicillin acylase, an enzyme found in some bacteria, yeast, and fungi that facilitates the production of several antibiotic precursors. (news-medical.net)
  • In industrial processes, protein hydrolysis is often carried out in batch processes and is mostly used in fermentation and enzyme based processes, like brewing, cheese manufacturing, meat tenderization or baking (Godfrey and Reichelt 1982 ). (springeropen.com)
  • Finally, the potential of Sso Pox-W263I to be active at subzero temperature is highlighted and discussed in regards to the common idea that hyperthermophile enzymes are nearly inactive at low temperatures. (nature.com)
  • Enzyme immobilization on electrically conductive supports is necessary to improve their bioactivity and stability, for use and re-use in applications depending on bio-electrochemical response, such as in bioelectrodes, biosensors, or biofuel cells. (diva-portal.org)
  • The results showed that bioactivity and bio-electro-activity of immobilized GOx was promising in color removal of Remazol Blue RR reactive dye and its partial degradation from solution in both treatments, which proved the success of the chosen immobilization methods in producing bioactive textiles that can be used as electrodes for power generation and pollution control. (diva-portal.org)
  • We tested enzyme resistance against diverse process-like and operating conditions such as heat resistance, contact with organic solvents, sterilization, storage and immobilization. (nature.com)
  • By means of modifying the amino resin carrier with the cross-linking agent treated by the polymer, the enzyme immobilized on the amino resin carrier may easily form a network cross-linking, such that the immobilization effect of the enzyme is more stable, thereby the recycling efficiency of the enzyme is improved. (justia.com)
  • These features increase the compatibility of these biocatalysts with industrial processes and are continuously under consideration for developing efficient enzyme-based technologies. (nature.com)
  • To reduce energy and enzyme costs and produce continuously defined hydrolysates, the enzymes need to be stabilized. (springeropen.com)
  • This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO). (justia.com)
  • The immobilized enzyme includes an enzyme and an amino resin carrier for immobilizing the enzyme, and the enzyme is selected from any one of the following enzymes: transaminase, ketoreductase, monooxygenase, ammonia-lyase, ene reductase, imine reductase, amino acid dehydrogenase, and nitrilase. (justia.com)
  • Biotechnology then includes the application of mechanisms uncovered within the field of biochemistry for the production of a useful product and the use of biochemical techniques in combination with physical analysis methods for a better understanding of biochemistry. (news-medical.net)
  • The most common method is to immobilize the enzymes on a solid support (Moehlenbrock and Minteer 2011 ). (springeropen.com)
  • Selection of mutants bearing favorable qualities by humans followed by repeated error-prone PCR allowed the field of directed evolution to produce enzymes with much greater thermal and chemical stability, and when combined with the recombinant protein technology already developed, massively widened the useful application of enzymes in industry. (news-medical.net)
  • The enzyme is able to retain its activity over 20 cycles of use, which though impressive, still forces manufacturers to utilize lower-cost stationary phases. (news-medical.net)
  • Owing to its bi-functional catalytic abilities as well as its intrinsic stability, Sso Pox is appealing for many applications, having potential uses in the agriculture, defense, food and health industries. (nature.com)
  • As discussed, enzymes generally have greater specificity, lower energy thresholds, and better environmental stability than comparable synthetic chemical catalysts. (news-medical.net)
  • Since the emergence of directed evolution and the beginning of the third wave of biocatalysis, enzymes have gained considerable interest for biotechnological purposes 1 . (nature.com)
  • The use of enzymes within the industry is a prominent example of a biochemical process that can be applied to biotechnology, potentially offering an environmentally friendly and highly efficient alternative to traditional chemical synthesis. (news-medical.net)