Apoptotic known cell cycle. Medical search. Frequent questions

Despite its pro-apoptotic role, caspase-2-deficient tumors have comparable basal apoptosis levels to wild-type tumors [3, 4]. (nature.com)
These cells do not undergo apoptosis, and the primary outcome of caspase-2 activation is continued, cell division. (nature.com)
This indicates a role distinct from caspase-2's reported role in inducing apoptosis to remove damaged or aneuploid cells [7][8]. (nature.com)
therefore, we overexpressed Bcl-X L to distinguish caspase-2's role in cell division and DNA protection from its role in apoptosis. (nature.com)
While other groups have demonstrated non-apoptotic roles for caspase-2 in DNA repair, cell cycle arrest [2], this is the first demonstration that these roles are regulated by a distinct pathway to apoptosis. (nature.com)
If apoptosis is not an essential mechanism for its tumor suppression function, then a role in regulating the cell cycle may be. (nature.com)
Plagioneurin B was isolated via conventional chromatography and tested for thorough mechanistic apoptosis activity on human ovarian cancer cells (CAOV-3). (um.edu.my)
The existence of apoptotic bodies, cell membrane blebbing and chromatin condensation indicated the hallmark of apoptosis. (um.edu.my)
Increase of Annexin V-FITC bound to phosphatidylserine confirmed the apoptosis induction in the cells. (um.edu.my)
Vpr also induces G2 cell cycle arrest and apoptosis in proliferating cells, which can result in immune dysfunction. (wikipedia.org)
p53 is an important tumor suppressor which induces either cell cycle arrest or apoptosis in response to DNA damage. (wikipedia.org)
Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
Although the conventional activities of p53 such as cell cycle arrest, senescence, and apoptosis are well accepted as the major checkpoints in stress responses, accumulating evidence implicates the importance of other tumor suppression mechanisms. (nature.com)
Is p53-dependent ferroptosis sufficient for tumor suppression in the absence of cell cycle arrest, senescence, and apoptosis? (nature.com)
To date, various mechanisms have been suggested to explain the powerful tumor-suppressive effect of p53, including the induction of cell cycle arrest, senescence, and apoptosis. (nature.com)
Apoptosis of the cells was analyzed by flow cytometry using an Annexin V and propidium iodide binding method. (nel.edu)
Total apoptosis (about 12%) and no significant alteration in cell cycle were confirmed for SGE of A. variegatum and R. appendiculatus, and additionally SGE of 5-day fed I. ricinus females (IC(50) 0.64 mg/ml). (nel.edu)
Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
Khz inhibited cell division and induced apoptosis in A549 cells. (springer.com)
Induction of apoptosis by ethanol extracts of Ganoderma lucidum in human gastric carcinoma cells. (springer.com)
Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. (springer.com)
Here, we explored whether sesamin activates p53, which is widely inhibited in cervical cancer cells, thereby inducing p53-mediated apoptosis. (medsci.org)
Cell proliferation, cell cycle distribution, and apoptosis were evaluated by the CCK-8 assay and flow cytometry using PI/Annexin V staining, respectively. (medsci.org)
Pifithrin-α also reduced apoptosis and restored the proliferation of HeLa and SiHa cells exposed to sesamin. (medsci.org)
These findings indicate that sesamin inhibits cervical cancer cell proliferation, and its mechanism may be attributed to the induction of p53/PTEN-mediated apoptosis. (medsci.org)
Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. (hindawi.com)
Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
We also observed that expression of Cp protein in mouse muscle resulted in apoptosis and inflammation of muscle cells. (cdc.gov)
In vitro apoptosis in individual cells was determined by terminal desoxyriboxyl-desoxyriboxyl transferase-mediated DVTP nick-end labeling (TUNEL) assay with the In Situ Cell Death Assay Kit (Roche Diagnostic Corp., Indianapolis, IN) and visualized by fluorescent microscopy. (cdc.gov)
New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
In the present study, we analyzed the proliferation and apoptosis of trophoblasts and human decidual cells in patients with recurrent spontaneous abortion and the related cellular pathway mechanism. (spandidos-publications.com)
The mechanism of recurrent abortion is considered to be related to the proliferation and apoptosis of human decidual cells and cytotrophoblasts ( 4 ). (spandidos-publications.com)
TP53 protein can induce cell growth arrest, apoptosis, differentiation and DNA repair, and activate or inhibit the expression of downstream genes such as Bax and CDKN1A ( 5 ). (spandidos-publications.com)
In the present study, we comparatively analyzed the cell apoptosis and cell signaling pathways of healthy patients and those with recurrent spontaneous abortion, providing a theoretical basis for clinical treatment. (spandidos-publications.com)
Interestingly, rbf1 -induced apoptosis leads to a debcl - and drp1 -dependent Reactive Oxygen Species production, which in turn activates the Jun Kinase pathway to trigger cell death. (sdbonline.org)
Reactivation of p53 in established tumors typically results in one of two cell fates, cell cycle arrest or apoptosis, but it remains unclear how this cell fate is determined. (nih.gov)
We hypothesized that high mitochondrial priming prior to p53 reactivation would lead to apoptosis, while low priming would lead to survival and cell cycle arrest. (nih.gov)
Using a panel of Kras-driven, p53 restorable cell lines derived from genetically engineered mouse models of lung adenocarcinoma and sarcoma (both of which undergo cell cycle arrest upon p53 restoration), as well as lymphoma (which instead undergo apoptosis), we show that the level of mitochondrial apoptotic priming is a critical determinant of p53 reactivation outcome. (nih.gov)
Cells with high initial priming (e.g., lymphomas) lacked sufficient reserve antiapoptotic capacity and underwent apoptosis after p53 restoration. (nih.gov)
To pursue the goals of this project, isogenic cell lines along with techniques to assess growth control, apoptosis and DNA repair will be employed. (europa.eu)
However, since the developmental defects observed in Bat3-null mouse embryos cannot be explained solely by defects in apoptosis, we investigated whether BAT3 is also involved in cell-cycle progression. (duke.edu)
2013). Apoptosis is induced intrinsically by the release of pro-apoptotic proteins from the inter-membrane space of the mitochondrion (eg. (uni-muenchen.de)
As described in the literature, wildtype p53 leaded to an increase in intrinsic cell death, whereas different p53 mutations reduced apoptosis. (uni-muenchen.de)
As a next step we studied the influence of cell cycle arrest induced by p53 activation on TRAIL apoptosis sensitivity (Ehrhardt/Wachter et al. (uni-muenchen.de)
2013). Cell cycle arrest was induced in tumor cell lines and patient samples in G0, G1 or G2 with cytostatic drugs, phase-specific inhibitors or RNA interference against cyclinB and E. Molecular or biochemical arrest at any point of the cell cycle augmented the rate of apoptosis by TRAIL. (uni-muenchen.de)
The extrinsic apoptosis by TRAIL was increased in senescent tumor cells. (uni-muenchen.de)
The impact of the p53 status of tumor cells on extrinsic apoptosis signaling should be considered, especially in the context of therapeutic approaches that aim to restore p53 function to enable cell death. (uni-muenchen.de)
The contributors describe strategies for identifying and evaluating the biochemical and morphological changes that occur during apoptosis, autophagy, necrosis, and other forms of cell death, as well as for pinpointing the genes and proteins that may be involved. (cshlpress.com)
It is known to induce apoptosis in some cell lines. (agscientific.com)
For example, it is reported that ionomycin promotes apoptosis in immature B cells and regulates cell cycle in mature B cells. (agscientific.com)
A significant increase in the G1 phase cell population was observed in MCF‑7 cells and ACT‑3 induced apoptosis by reducing the ratio of B‑cell lymphoma‑2 (Bcl‑2)/Bcl‑2‑associated X (Bax) and releasing cleaved caspase 9. (iasp-pain.org)
Thus, the activated ERK pathway played an important role in cell cycle arrest and apoptosis via ERK‑dependent induction of p21 in MCF‑7 cells. (iasp-pain.org)
Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
Lastly, hydroquinone and NU7026 exhibited synergistic effects on promoting apoptosis by increasing the protein levels of pro-apoptotic proteins Bax and caspase-3 but decreasing the protein expression of anti-apoptotic protein Bcl-2. (cdc.gov)
Taken together, the findings reveal a central role of DNA-PKcs in hydroquinone-induced hematotoxicity in which it coordinates DNA DSB repair, cell cycle progression, and apoptosis to regulate the response to hydroquinone-induced DNA damage. (cdc.gov)
activities of the former include cell cycle progression inhibition, tumor microenvironment preparation, apoptosis promotion, stimulation of cancer cell invasion and metastasis, and suppression of immune surveillance. (bvsalud.org)
Normally, these cells are eliminated by apoptosis. (medscape.com)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

VprBP also regulates p53-induced transcription and apoptotic pathways. (wikipedia.org)
A re-evaluation of parameters of cell kinetics in view of our increasing knowledge of the molecular pathways of cell cycle control may provide more prognostic information for the management of patients with malignant lymphomas. (bmj.com)
Once activated, p53 coordinates multiple downstream pathways, thereby maintaining the homeostasis of the host cell or organism (if the stress is mild, transient, and repairable) or eliminating damaged cells (if the stress is acute, prolonged, and difficult to resist). (nature.com)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
There are multiple types of cell death, several interconnected signaling pathways can be activated, and the postmortem consequences may vary. (cshlpress.com)
1. Role of the cytokine-signal transduction pathways and epigenetic gene silencing in pathogenesis of T-cell lymphoma. (upenn.edu)
It is likely that in all of these syndromes, tumours develop against a background created by loss of the growth-suppressive function of their susceptibility gene via mechanisms including disruption of the cell cycle and the activation of anti-apoptotic pathways. (nih.gov)
Anticancer effects of the HDAC inhibitor, 3β,6β‑dihydroxyurs‑12‑en‑27‑oic acid, in MCF‑7 breast cancer cells via the inhibition of Akt/mTOR pathways. (iasp-pain.org)
modulate cell cycle proteins, and inhibit apoptotic pathways. (bvsalud.org)
Several pathways involved in cell cycle and DNA repair were affected upon NV651 treatment. (lu.se)

T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
Khz reduced MMP and Bcl-2 protein levels, but increased ROS generation and p53 and pro-apoptotic proteins. (springer.com)
It has been previously shown that rbf1 pro-apoptotic activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. (sdbonline.org)

Measurement of apoptotic markers in tumors can be directly correlated with the cell cycle phase using flow cytometry (FCM). (edu.au)
Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. (springer.com)
Flow cytometry analysis showed that the percentage of A549 cells in sub-G1 phase of the cell cycle increased in response to Khz treatment. (springer.com)
Methods: Human NET cell lines BON1, QGP-1, and NCI-H727 were treated with trametinib or SCH772984, alone and in combination with ribociclib, to assess cell proliferation, cell cycle distribution, and protein signaling using cell proliferation, flow cytometry, and Western blot assays, respectively. (uni-muenchen.de)
Based on the significant antibacterial activity, the sorting of bacterial cells against the compounds (MID6 and MID9) was analyzed by flow cytometry. (degruyter.com)
Flow cytometry, caspase-3 activity assay in treated monolayers/spheroids and also fluorescence staining and DNA fragmentation in treated monolayers demonstrated apoptotic death mode. (irost.org)
In the present study, the effects of ACT‑3 on anticancer effects of MCF‑7 cells were determined by measuring the level of apoptotic cell death and cell cycle regulator using flow cytometry analysis and western blot analysis, respectively. (iasp-pain.org)

Using mutational analysis (biochemical identification of mutational changes in a nucleotide sequence), prevention of cell cycle progression into mitosis was shown to be required for LTR-mediated viral expression. (wikipedia.org)
During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
Increased levels of polyamines in cells are considered to be involved in cancer progression. (spandidos-publications.com)
Our findings indicate that in Bat3-knockdown cells, p21 continues to be synthesized during cell-cycle phases that do not normally require p21, resulting in p21 protein accumulation and a subsequent delay in cell-cycle progression. (duke.edu)
Finally, we showed that BAT3 co-localizes with p21 during the cell cycle and is required for the translocation of p21 from the cytoplasm to the nucleus during the G1/S transition and G2/M progression. (duke.edu)
We found that cutaneous T-cell lymphoma cells display activation of the interleukin-2 receptor/cytokine common chain-associated Jak/STAT signal transduction pathway that is transient in the early stage of the lymphoma and constitutive in the late stage of the disease progression. (upenn.edu)
Our studies indicate that RAD has a strong inhibitory effect on PTLD-like and PTLD-derived B cells by suppressing their proliferation, blocking cell cycle progression and increasing apoptotic rate. (upenn.edu)
Cell cycle progression revealed no statistically significant variation. (bvsalud.org)
Herceg, Z., Hulla W., Gell D., Cuenin C., Lleonart M., Jackson S. and Wang Z.Q. (2001) Disruption of Trrap causes early embryonic lethality and defects in cell cycle progression. (who.int)

We propose a model where caspase-2 regulates the cell cycle and protects DNA replication forks to prevent DNA damage accrual in a pathway distinct from its apoptotic role. (nature.com)
If caspase-2 regulates cell division through the same pathway, it would be expected to phenocopy the cell cycle results of caspase-2 null cells. (nature.com)
In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
This study may result in novel therapies for lymphoma based on selective inhibition of the elements of the IL-2R signal transduction pathway(s) which are preferentially utilized by malignant T cells and/or on induction of re-expression of the epigenetically-silenced SHP-1 gene. (upenn.edu)
Additionally, ACT‑3 significantly increased autophagic cell death by inhibiting the serine‑threonine kinase/mammalian target of the rapamycin pathway. (iasp-pain.org)
We could confirm the increased polyploidy upon Mps1 inhibition in neuroblastoma cell lines and PDX model, leading to mitotic catastrophe activating the caspase-dependent mitochondrial apoptotic pathway. (lu.se)

1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
Differential regulation by melatonin of cell growth and androgen receptor binding to the androgen response element in prostate cancer cells. (nel.edu)
Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)
Genes for non-apoptotic cell death and regulation of osmotic pressure were down-regulated. (wada-ama.org)
CONCLUSION: Our study reveals a novel, non-apoptotic role for BAT3 in cell-cycle regulation. (duke.edu)
Aberrant cell signaling and epigenetic regulation of gene expression in human lymphomas. (upenn.edu)
Regulation and function of STAT3 in the ALK+ T-cells and testing an ALK-inhibitor small molecule candidate are the main focus of the ongoing investigation. (upenn.edu)

Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)
The anti-proliferative and apoptotic potential of salivary gland extracts (SGE) of unfed and partially fed adult ixodid ticks were screened using human HeLa cells. (nel.edu)
Kazimírová M, Dovinová I, Rolníková T, Tóthová L, Hunáková L. Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)
This study aimed to investigate the anti-proliferative effects of Khz on A549 lung cancer cells. (springer.com)
The anti-proliferative and pro-apoptotic effects of Khz suggest that this extract shows great promise as a potential chemotherapeutic agent. (springer.com)
Scholars@Duke publication: A novel, non-apoptotic role for Scythe/BAT3: a functional switch between the pro- and anti-proliferative roles of p21 during the cell cycle. (duke.edu)
BAT3 modulates these pro- and anti-proliferative roles of p21 at least in part by regulating cyclin A abundance, as well as p21 translocation between the cytoplasm and the nucleus to ensure that it functions in the appropriate intracellular compartment during each phase of the cell cycle. (duke.edu)

Mitochondrial apoptotic priming is a key determinant of cell fate upon p53 restoration. (nih.gov)

Cell growth inhibition activity of SGE was measured by the MTT assay. (nel.edu)
Inhibition of p53 by pifithrin-α significantly reduced the levels of PUMA, Bax, and PTEN but restored AKT phosphorylation in SiHa cells exposed to sesamin. (medsci.org)
Conclusions: MEK and ERK inhibition causes antiproliferative effects in human NET cell lines in vitro. (uni-muenchen.de)
Therefore, we used OEO and its main component, thymol, to explore their effects on cell growth inhibition and anticancer activity. (irost.org)
Inhibition of DNA-PKcs by NU7026 markedly potentiated the apoptotic and growth inhibitory effects of hydroquinone in proerythroid leukemic K562 cells in a dose-dependent manner. (cdc.gov)

In the in vivo SCID mouse xeno-transplant model, RAD markedly delayed growth or induced regression of established PTLD-related B-cell tumors. (upenn.edu)
This review summarizes the molecular mechanisms of TGF-ß signal transduction, focusing on oral squamous cell and salivary adenoid systemic carcinomas as well as keratocystic odontogenic tumors. (bvsalud.org)
Extragonadal germinal cell syndromes are rare tumors that predominantly affect young males. (medscape.com)
Literature suggests that the only known risk factor for extragonadal germ cell tumors (EGCTs) is Klinefelter syndrome (47XXY), which is associated with mediastinal nonseminomatous germ cell tumors. (medscape.com)
In extragonadal germ cell tumors, no evidence of a primary malignancy is present in either the testes or ovaries by radiologic imaging or physical examination. (medscape.com)
Extragonadal germ cell tumors produce a rich symptomatology and may reach large volumes if they arise in silent areas. (medscape.com)
Controversy remains regarding the origin of extragonadal germ cell tumors (EGGCTs). (medscape.com)
The classic theory suggests that germ cell tumors (GCTs) in these areas are derived from local transformation of primordial germ cells misplaced during embryogenesis. (medscape.com)
According to this theory, the differences in phenotypes expressed by mediastinal germ cell tumors (MGCTs) and gonadal germ cell tumors may be explained by differences in the cellular environment between the gonad and the anterior mediastinum. (medscape.com)
Some retroperitoneal extragonadal germ cell tumors may represent metastases from a testicular cancer , with subsequent spontaneous necrosis of the primary tumour. (medscape.com)
Hematologic malignancies are frequently associated with mediastinal germ cell tumors. (medscape.com)
Highly differentiated yolk-sac tumors make up 30% of mediastinal germ cell tumors, providing a possible basis for this association. (medscape.com)
The balance of the p53-mdm2 interaction has been shown to be disrupted in intracranial germ cell tumors (ICGCTs). (medscape.com)

These non-apoptotic mechanisms may underlie the role of caspase-2 as a tumor suppressor. (nature.com)
Our most recent work focuses on the molecular mechanisms of the aberrant gene silencing in the malignant lymphoid cells. (upenn.edu)
3. Mechanisms of malignant cell transformation by the chimeric NPM/ALK kinase. (upenn.edu)
In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)

Expression of ALK in malignant T cells is typically due to the t(2;5) translocation resulting in formation of the fusion gene which encodes a 80-kDa hybrid protein that contains portion of the nuclear protein nucleophosmin (NPM) joined to the entire cytoplasmic portion of the receptor tyrosine kinase ALK. (upenn.edu)
Malignant transformation of germ cells is the result of a multistep process of genetic changes. (medscape.com)
Non-Hodgkin Lymphomas Non-Hodgkin lymphomas are a heterogeneous group of disorders involving malignant monoclonal proliferation of lymphoid cells in lymphoreticular sites, including lymph nodes, bone marrow, the. (msdmanuals.com)
Burkitt lymphoma is the most rapidly growing human tumor, and pathology reveals a high mitotic rate, a monoclonal proliferation of B cells, and a "starry-sky" pattern of benign macrophages that have engulfed apoptotic malignant lymphocytes. (msdmanuals.com)

These studies demonstrate that the capsid protein of WNV may be responsible for aspects of viral pathogenesis through induction of the apoptotic cascade. (cdc.gov)
In the present study, our aim was to determine the time-dependent, ER-mediated apoptotic and autophagy induction of purvalanol in HCT 116 colon cancer cells. (spandidos-publications.com)
In our work we discovered that the influence of p53 status on extrinsic cell death induction was much more multifaceted. (uni-muenchen.de)

Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells. (nel.edu)
In this study, we investigated the effect of this anticancer drug alone and in combination with a histone deacetylase (HDAC) inhibitor, valproic acid (VPA), on a human UKF-NB-4 neuroblastoma cell line. (nel.edu)
Groh T, Hrabeta J, Poljakova J, Eckschlager T, Stiborova M. Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells. (nel.edu)
The involvement of p53 in the apoptotic cascade was assessed by a specific inhibitor. (medsci.org)
Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
In this study we investigated the effects of the MEK inhibitor trametinib, the ERK inhibitor SCH772984 and the CDK4/6 inhibitor ribociclib in human neuroendocrine tumor cell lines BON1, QGP1 and NCI-H727 in vitro. (uni-muenchen.de)
Objectives: This study assessed the antitumoral activity of the MEK inhibitor trametinib (TMT212) and the ERK1/2 inhibitor SCH772984, alone and in combination with the CDK4/6 inhibitor ribociclib (LEE011) in human neuroendocrine tumor (NET) cell lines in vitro. (uni-muenchen.de)
The molecular mechanism of this TOR inhibitor-mediated cell growth suppression is currently under investigation. (upenn.edu)
Treatment with the novel cyclophilin inhibitor NV651, presented a potent antiproliferative effect in HCC cell lines via cell cycle perturbations arresting cells in the mitotic phase. (lu.se)

We evaluated the effect of a CTS, cinobufotalin (CINO), on CTB cell function in vitro. (tamu.edu)
The lack of an in vitro cell culture system that demonstrated a deficit in replication upon infection with viruses in the absence of Vpr has led to some mystery in the function of Vpr. (wikipedia.org)
Recently, there has been experiments on monocyte-derived dendritic cells (MDDCs) using a novel in-vitro infection system. (wikipedia.org)
Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
In the first phase of the project cell lines of specific leukocyte subsets (THP-1/monocyte, IM-9/T lymphocyte, H9/B lymphocyte), PBMCs (peripheral blood mononuclear cells) from healthy donors, respectively will be treated in vitro with hgh to identify candidate genes influenced by hghapplication. (wada-ama.org)
To this purpose cell lines of specific leukocyte subsets (THP-1/ monocyte, IM-9/ B- lymphocyte, H9/ T-lymphocyte) and PBMCs (peripheral blood mononuclear cells) from healthy donors, respectively were treated in vitro with hgh to identify candidate hgh-sensitive genes. (wada-ama.org)
In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA. (irost.org)
NV556 treatment in an in vitro 3D human liver Extracellular Matrix (ECM) cultured with a TGFβ1-activated human hepatic stellate cell line led to decreased collagen production. (lu.se)

Cell cycle analysis and quantification of DNA fragmentation was performed by DNA staining with propidium iodide. (nel.edu)

Fifteen micromoles of purvalanol induced a reduction in cell viability by 20 and 35% within 24 and 48 h, respectively. (spandidos-publications.com)
Results: Trametinib and SCH772984, alone and in combination with ribociclib, significantly reduced NET cell viability and arrested NET cells at the G1 phase of the cell cycle in all three cell lines tested. (uni-muenchen.de)
A significant decrease (0.05-0.5 μM) was observed in the cell viability and IC 50 dosage was fixed as 0.25 μM after 24 h of treatment. (degruyter.com)
This indispensable laboratory resource will enable all cell and developmental biologists, from the graduate level upward, to confidently carry out and comprehend a wide array of cell viability assays. (cshlpress.com)
ACT‑3 significantly decreased the cell viability in a concentration‑dependent manner and induced different morphological changes at high concentrations. (iasp-pain.org)
The combination of NV651 and cisplatin, a DNA damage reagent, resulted in a synergistic effect in cell viability, and a significant increase on cell death in HCC cell lines. (lu.se)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

T lymphocytes for adoptive cell therapy against hematological cancer. (upstate.edu)
Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)

These findings indicate that TOR inhibitors such as RAD may be effective in prevention and treatment of PTLDs and, possibly, other types of B-cell lymphoma. (upenn.edu)
Accumulating evidence indicates that expression of anaplastic lymphoma kinase (ALK) defines a distinct type of T-cell lymphoma. (upenn.edu)
Burkitt lymphoma is an aggressive B-cell non-Hodgkin lymphoma occurring in children and adults. (msdmanuals.com)
Clinically, grade 3B follicular lymphoma is treated like diffuse large B-cell lymphoma . (medscape.com)

Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle. (springer.com)
Genes clustering in the categories fatty acid beta oxidation, cell adhesion, DNA replication and polyamine biosynthesis were up-regulated indicating increased lipolysis, cell attachment, proliferation and growth. (wada-ama.org)

Vpr stands for "Viral Protein R". Vpr, a 96 amino acid 14-kDa protein, plays an important role in regulating nuclear import of the HIV-1 pre-integration complex, and is required for virus replication and enhanced gene expression from provirus in dividing or non-dividing cells such as T cells or macrophages. (wikipedia.org)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)

Stimulation of caspase 3 and the presence of DNA ladder suggested downstream apoptotic signalling were initiated. (um.edu.my)

Among these unconventional activities, an iron-dependent form of non-apoptotic cell death, termed ferroptosis, attracts great interest. (nature.com)
By inhibiting the topoisomerase II enzyme activity in cancer cells, this drug leads to DNA damage and subsequently to cell death. (nel.edu)
Programmed cell death and Bcl-2 protection in very low oxygen. (springer.com)
Jacobson MD. Reactive oxygen species and programmed cell death. (springer.com)
We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)
Moreover, we determined that during purvalanol-mediated ER stress, autophagic machinery was also activated prior to apoptotic cell death finalization. (spandidos-publications.com)
Τheir impact on the apoptotic cell death mechanism requires further elucidation. (spandidos-publications.com)
MID9 showed a moderate level of cell death compared to the control. (degruyter.com)
doxorubicin) or via activation of death receptors on the surface of the cell (eg. (uni-muenchen.de)
The presence of p53 in tumor cell lines and xenograft tumor cells resulted in augmented, unaffected or decreased cell death. (uni-muenchen.de)
Regulated cell death, which is involved in nearly every aspect of animal development and physiology, can be challenging to study in the laboratory. (cshlpress.com)
Therefore, a combination of specialized techniques is often required to characterize and confirm cell death in the system of interest. (cshlpress.com)
This laboratory manual provides a comprehensive suite of step-by-step protocols for inducing, detecting, visualizing, characterizing, and quantifying cell death in a variety of systems. (cshlpress.com)
The authors also provide guidance on interpreting and presenting the results of cell death experiments, as well as advice on complementary procedures that may be required to confirm the results of a given experiment. (cshlpress.com)
Infants suffering of cell death occurring as a series of changes from chronic illnesses, chromosomal abnor- in dying cells under several physiological malities and those on medication prior to conditions [ 6,7 ] and has been implicated enrolment were excluded from the study. (who.int)
The cell carrying this abnormality is relatively protected against apoptotic death because of the oncogenic effect of CCND2, leading to re-initiation of cell cycle and genomic instability. (medscape.com)
Cyclophilin overexpression is a common event in fibrotic tissues playing a key role in different stages of the fibrotic process, including inflammation, hepatocyte death, and activation of the hepatic stellate cells leading to increased collagen production. (lu.se)
2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)

Acetogenin group has attracted worldwide attention in the past few years due their biological abilities as inhibitors for several types of tumour cells. (um.edu.my)
The results found here warrant further studies of combined treatment of neuroblastoma cells with etoposide with HDAC inhibitors and may help in the design of new protocols geared to the treatment of high risk neuroblastomas. (nel.edu)
Olomoucine, roscovitine and purvalanol are examples of CDK inhibitors (CDKIs) designed and investigated for their apoptotic potential on cancer cells ( 3 ). (spandidos-publications.com)

It has long been noted that caspase-2-deficient cells proliferate faster [2], and elegant studies have shown that caspase-2 induces cell cycle arrest following cytokinesis failure [5]. (nature.com)
While it is well understood that doxorubicin activates p53 and induces cell-cycle arrest, we observed that cell-cycle arrest disabled inactivation of anti-apoptotic Bcl-2-family members by vincristine. (uni-muenchen.de)

SGE of all ticks examined suppressed HeLa cell proliferation in a dose dependent manner. (nel.edu)
Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells. (springer.com)
Human HeLa and SiHa cervical cancer cells and normal Hs68 dermal cells were used as cell models. (medsci.org)
Sesamin (75 and 150 μM) clearly inhibited SiHa and HeLa cell proliferation in a dose-dependent fashion, but did not affect the proliferation of Hs68 cells. (medsci.org)
The localization pattern of capsid expression was analyzed by immunofluorescent assay in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag antibody as described ( 6 ). (cdc.gov)

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. (cancerindex.org)

However, proliferative indices alone do not suffice to characterise cell growth. (bmj.com)
rbf1 , the Drosophila homolog of Rb , also displays a pro- apoptotic activity in proliferative cells. (sdbonline.org)

Miyajima, A, Nakashima J, Yoshioka K. Role of reactive oxygen species in cis-dichlorodiammineplatium-induced cytotoxicity on bladder cancer cells. (springer.com)
Indeed, OEO/thymol increased the Reactive Oxygen Species (ROS) level leading to mitochondrial membrane potential (MMP, ΔΨm) loss, caspase-3 activation and DNA damage caused S-phase cell cycle arrest. (irost.org)

In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
The cytotoxicity of etoposide and VPA to a UKF-NB-4 neuroblastoma cell line was evaluated with MTT assay. (nel.edu)
They also indicate that the impact of VPA on cytotoxicity of etoposide in these tumor cells varies depending on the sequence of cultivation of the cells with the drugs. (nel.edu)
In contrast, the reversed combination (preincubation of the cells with VPA before their treating with etoposide) did not give any increase in etoposide cytotoxicity. (nel.edu)
Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
Despite having a limited effect on L929 normal cells, OEO/thymol induced cytotoxicity in MDA-MB231 breast cancer monolayers (2D) and to a lesser extent in MDA-MB231 spheroids (3D). (irost.org)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)

The section thickness was 5 µm, and was placed on a glass slide with APES for immunohistochemistry and apoptotic staining. (spandidos-publications.com)

It is also believed that the ionophore induces apoptotic degeneration of embryonic cortical neurons. (agscientific.com)

By maintaining a low p21 protein level during the G1/S transition, BAT3 counteracts the inhibitory effect of p21 on DNA replication and thus enables the cells to progress from G1 to S phase. (duke.edu)
The purpose of the present study was to investigate the histone deacetylase (HDAC) inhibitory and anticancer activities of ACT‑3 compared with suberoylanilide hydroxamic acid (SAHA) in MCF‑7 human breast cancer cells. (iasp-pain.org)

The data show that μFCM can provide a viable novel alternative to conventional FCM for multiparameter detection of caspase activation and dissipation of mitochondrial inner membrane potential (ΔΨm) in relation to DNA content (cell cycle phase) in live tumor cells. (edu.au)

Cells with low initial priming (e.g., lung adenocarcinoma and sarcoma) survived and proceeded to arrest in the cell cycle. (nih.gov)
Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)

In our paper published in Oncogene [6], we show that caspase-2 plays an essential role in regulating normal cell division by protecting DNA replication forks from replication stress. (nature.com)
We show that caspase-2 protects cells from a range of S-phase-related defects, including delayed exit from S-phase, increased stalled replication forks, increased new replication origins, and decreased repair of S-phase-associated chromosomal aberrations [6]. (nature.com)
Consistent with this, we noted a caspase-2-dependent slowdown of the cell cycle following mild replication stress. (nature.com)
Consistent with our evidence that caspase-2 regulates a replication checkpoint, the chromosomal aberrations detected in caspase-2-deficient cells are not repaired before metaphase. (nature.com)
These infected human dendritic cells showed a slower rate of replication when deprived of the Vpr protein in HIV-1 cells. (wikipedia.org)
These findings suggest that the evolutionarily secured G2 cell cycle arrest function of Vpr (Viral Protein R) is essential for HIV-1 replication. (wikipedia.org)

HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
In addition, trametinib also caused subG1 events and apoptotic PARP cleavage in QGP1 and NCI-H727 cells. (uni-muenchen.de)
Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)

Nestin is an intermediate filament protein known to play several roles, including as a marker of neural progenitor and proliferating endothelial cells. (molvis.org)

TERT promoter mutations in head and neck squamous cell carcinoma: A systematic review and meta-analysis on prevalence and prognostic significance. (nih.gov)
This study aims to describe our clinical institutional experience based on the multimodal treatment performed in four cases diagnosed with oral squamous cell carcinoma with the use of different microvascular free flaps and RT. (bvsalud.org)

The MTT assay was used to examine the cytotoxic effects of fungal taxol against the human liver cancer cell line HepG2. (degruyter.com)

Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. (nih.gov)
Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. (nih.gov)
TERT expression is associated with metastasis from thin primaries, exhausted CD4+ T cells in melanoma and with DNA repair across cancer entities. (nih.gov)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
A western blot analysis demonstrated the use of trametinib alone and trametinib in combination with ribociclib to decrease the expression of pERK, cMyc, Chk1, pChk2, pCDK1, CyclinD1, and c-myc in a time-dependent manner in NCI-H727 and QGP-1 cells. (uni-muenchen.de)
After any retinal injury in experimental conditions, glial cells are activated and undergo reactive gliosis with increased an expression level of glial fibrillary acid protein (GFAP), which leads to overgrowth of outer Müller glial cell processes. (molvis.org)
Whole genome cDNA microarrays as well as oligonucleotide microarrays including leukocyte-relevant genes will be used to characterize potential hgh-specific genes by comparing gene expression profiles of treated and untreated cells. (wada-ama.org)
Forced BCL-2 or BCL-XL expression reduced priming and resulted in survival and cell cycle arrest. (nih.gov)
More recently we determined that the constitutive Jak/STAT activation is due, at least in part, to the lack of expression of SHP-1 phosphatase, which normally down-regulates IL-2R/ c-mediated cell activating signals. (upenn.edu)

The hypothesis to be tested is that ALDH3A1 modulates cell cycle and apoptotic cascades in order to allow for DNA repair, and thus, promotes cell survival in response to UV-induced oxidative damage. (europa.eu)

The effect of etoposide and VPA on the cell cycle distribution was determined by flow cytometric analysis using propidium iodide staining. (nel.edu)

Our findings showed that plagioneurin B successfully inhibits the growth of CAOV-3 cells at IC 50 of 0.62 µM. (um.edu.my)
Khz, a naturally occurring compound derived from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia, inhibits the growth of cancer cells. (springer.com)

F) The vast majority of -gal+ (PR+) cells in various regions in PRPL mice are neurons, as evidenced by co-labeling with NeuroTrace (fluorescent Nissl) in the arcuate and NeuN for the remaining regions. (cdc.gov)

CDKs show their action by interacting with cyclins and different CDK-cyclin complexes regulate the cell cycle in the G1, S and G2/M phases ( 1 ). (spandidos-publications.com)
In occasional cells, this crossing over may lead to increased 12p copy number and overexpression of cyclin D2. (medscape.com)

Targeting the cell cycle has been proposed as a therapeutic strategy in cancer treatment with special interest in the mitotic phase. (lu.se)

However, a recently proposed novel role of ALDH3A1 as a negative cell cycle regulator in corneal epithelial cells further supports its protective function in the cornea and reveals its multifunctional character. (europa.eu)

Zhao S, Ye G, Fu G, Cheng JX, Yang BB, Peng C. Ganoderma lucidum exerts anti-tumor effects on ovarian cancer cells and enhances their sensitivity to cisplatin. (springer.com)

Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)

As a suitable sequence of cultivation, with a high rate of suppression of neuroblastoma cell growth was found the preincubation of the cells with etoposide, which was followed by their cultivation with VPA. (nel.edu)

indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)

The first suggests that fetal gonocytes whose development into spermatogonia is blocked may undergo abnormal cell division and then invasive growth mediated by postnatal and pubertal gonadotrophin stimulation. (medscape.com)

2) Therefore, the proper control of protein levels is crucial for the cell cycle. (ukessays.com)

The feasibility of using the μFCM system for a dose-response profiling of investigational anti-cancer agents on human hematopoietic cancer cells is also demonstrated. (edu.au)
In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
Adjuvant therapy for breast cancer is designed to treat any potential micrometastatic disease, or breast cancer cells that have escaped the breast and regional lymph nodes but have not yet established an identifiable metastasis. (medscape.com)
Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Cycling cells are required for vincristine to kill cancer cells efficiently. (uni-muenchen.de)

Anatomy20

Organisms8

Diseases4

Chemicals and Drugs94

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes62

Disciplines and Occupations1

Information Science3

Health Care1

Apoptosis63

Pathways11

Proteins3

Flow cytometry7

Progression10

Pathway6

Regulation9

Anti-proliferative7

Mitochondrial apoptotic1

Inhibition5

Tumors13

Mechanisms4

Malignant4

Induction3

Inhibitor10

Vitro9

Fragmentation1

Viability7

Lymphocytes4

Lymphoma4

Proliferation and growth2

Macrophages2

Downstream1

Death18

Inhibitors3

Induces cell cycle2

HeLa5

Regulates cell1

Proliferative2

Reactive oxygen2

Cytotoxicity8

Vivo1

Immunohistochemistry1

Embryonic1

Inhibitory2

Membrane1

Lung2

Replication6

Cleavage4

Roles1

Squamous cell carc2

Assay1

Expression10

Allow for DNA repair1

Cytometric1

Inhibits2

Neurons1

Cyclin2

Mitotic1

Regulator1

Ovarian cancer cells1

Estimation1

Neuroblastoma cell1

Lymphoid1

Undergo1

Protein levels1

Cancer6