Apoptotic cells different phases cell cycle. Medical search. Frequent questions

Human cells with silenced MDC1 also displayed random plasmid integration, reduced apoptosis, and slowed mitosis. (wikipedia.org)
Pifithrin-α also reduced apoptosis and restored the proliferation of HeLa and SiHa cells exposed to sesamin. (medsci.org)

First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
MicroRNAs control the levels of numerous cell cycle regulators that controls cell proliferation. (ukessays.com)
The SignalStain ® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. (cellsignal.com)
Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)
Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
L-DOPA affects anterior pituitary cell proliferation and apoptosis L-3,4-dihydroxyphenylalanine (L-DOPA) is released by hypothalamic neurons. (conicet.gov.ar)
We investigated L-DOPA effects on the proliferation and apoptosis of anterior pituitary cells. (conicet.gov.ar)
In summary, our results indicate that L-DOPA affects anterior pituitary cell proliferation and apoptosis, and suggest that treatment with L-DOPA may have consequences in the neuroendocrine system. (conicet.gov.ar)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
In addition, cell proliferation and angiogenesis will be stimulated, enabling better oxygenation of the tissue. (bmj.com)
Methods: Human NET cell lines BON1, QGP-1, and NCI-H727 were treated with trametinib or SCH772984, alone and in combination with ribociclib, to assess cell proliferation, cell cycle distribution, and protein signaling using cell proliferation, flow cytometry, and Western blot assays, respectively. (uni-muenchen.de)
Purified cannabinoids have been reported to prevent proliferation, metastasis, and induce apoptosis in a variety of cancer cell types. (nih.gov)
Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
Cell proliferation, cell cycle distribution, and apoptosis were evaluated by the CCK-8 assay and flow cytometry using PI/Annexin V staining, respectively. (medsci.org)
Sesamin (75 and 150 μM) clearly inhibited SiHa and HeLa cell proliferation in a dose-dependent fashion, but did not affect the proliferation of Hs68 cells. (medsci.org)
These findings indicate that sesamin inhibits cervical cancer cell proliferation, and its mechanism may be attributed to the induction of p53/PTEN-mediated apoptosis. (medsci.org)
Moreover, our study demonstrated that FKBPL could suppress the proliferation of lung ADC cells by delaying cell cycle G1/S phase transition. (jcancer.org)
We showed inhibition of cell proliferation that was time- and dose-dependent in 5RP7 cells for 24 and 48 h. (tubitak.gov.tr)
Binding of one molecule of eribulin to two microtubules can inhibit cell proliferation by 50%, and such binding is reversible. (shu.edu)
In this report, we identified that KPNA3 was significantly upregulated in cancer cells, particularly in triple-negative breast cancer, and its knockdown resulted in the suppression of cell proliferation and metastasis. (bmbreports.org)
Genistein has been known to inhibit proliferation and induce apoptosis in several kinds of cancer cells. (biomedcentral.com)
Our previous study has proved GEN inhibits EGF-induced proliferation in colon cancer cells by promoting FOXO3 activity, targeting upstream the PI3K/Akt pathway [ 3 ]. (biomedcentral.com)
In this study, we demonstrated that GEN can inhibite proliferation and induce apoptosis of colon cancer cells by reversal of EMT via a Notch1/NF-κB/Slug/E-cadherin pathway. (biomedcentral.com)
Little is known about the early stages that regulate proliferation, differentiation, and survival of neural stem cells and their immediate progeny. (biomedcentral.com)

MDC1 has anti-apoptotic properties by directly inhibiting the apoptotic activity of the tumor suppressing protein p53. (wikipedia.org)
MDC1 can execute its anti-apoptotic activity by inhibiting p53 in two ways. (wikipedia.org)
Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). (cellsignal.com)
Using the Human Apoptosis Array Kit, we observed that overexpression of FKBPL in lung ADC A549 cells significantly decreased the anti-apoptotic proteins, including heat shock protein 32 (HSP32), heat shock protein 27 (HSP27), and paraoxonase-2 (PON2). (jcancer.org)

1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
Low ATP concentrations in hypoxic tumour cells disable the apoptotic cascade and induce cell death by necrosis. (bmj.com)
C. sativa compounds interact synergistically for cytotoxic activity against colon cancer cells and induce cell cycle arrest, apoptotic cell death, and distinct gene expression. (nih.gov)

In female mammals, a unique characteristic of meiosis, not observed in other types of cells, is the prolonged arrest during the prophase stage of meiosis I. In oocytes, DNA double-strand breaks can be repaired during meiosis I by a process involving microtubule-dependent recruitment of the CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes. (wikipedia.org)
A cell cycle arrest in early S-phase and in G 0 /G 1 -phase was also found after exposure to cisplatin and sunitinib malate, in isolation, respectively. (hindawi.com)
Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
Indeed, OEO/thymol increased the Reactive Oxygen Species (ROS) level leading to mitochondrial membrane potential (MMP, ΔΨm) loss, caspase-3 activation and DNA damage caused S-phase cell cycle arrest. (irost.org)
The F7 and F7+F3 cytotoxic activity involved cell apoptosis and cell cycle arrest in S or G0/G1 phases, respectively. (nih.gov)
When unreplicated or damaged DNA is present, cell cycle checkpoint pathways cause cell cycle arrest by inhibiting cyclin-dependent kinases (Cdks). (vt.edu)
Chk1 regulates cell cycle arrest in the presence of unreplicated DNA in somatic cells by phosphorylating Cdc25A and leading to its degradation. (vt.edu)
The cell cycle arrest caused by melatonin was observed in G1 and G2 phases in the cell cycle analyses in 5RP7 cells for 48 h. (tubitak.gov.tr)
This illustration shows the proposed relative location of the 2 known cell cycle arrest points in cells with MTD (microtubule damage). (shu.edu)
Plagioneurin B was also seen to arrest CAOV-3 cells cycle at the G2/M phase. (um.edu.my)
Further, flow cytometry analysis was done, suggesting an arrest of the cell cycle in the S phase of the HeLa cells. (bgu.ac.il)

MDC1 protein is a regulator of the Intra-S phase and the G2/M cell cycle checkpoints and recruits repair proteins to the site of DNA damage. (wikipedia.org)
It is involved in determining cell survival fate in association with tumor suppressor protein p53. (wikipedia.org)
The MDC1 gene encodes the MDC1 nuclear protein which is part of the DNA damage response (DDR) pathway, the mechanism through which eukaryotic cells respond to damaged DNA, specifically DNA double-strand breaks (DSB) that are caused by ionizing radiation or chemical clastogens. (wikipedia.org)
This protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. (wikipedia.org)
Studies on lung cancer cell lines (A549 cells) showed an increase in apoptosis in response to genotoxic agents when MDC1 protein levels were reduced with siRNA. (wikipedia.org)
Inhibition or loss of MDC1 protein through studies with siRNA on human cells or knockout studies in mice have shown several defects at both the cellular and organismal level. (wikipedia.org)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
2) Therefore, the proper control of protein levels is crucial for the cell cycle. (ukessays.com)
Nestin is an intermediate filament protein known to play several roles, including as a marker of neural progenitor and proliferating endothelial cells. (molvis.org)
Nestin was also observed in some vascular cells, as demonstrated by the co-immunoreactivity of the protein with vWF in the choroid and retina. (molvis.org)
After any retinal injury in experimental conditions, glial cells are activated and undergo reactive gliosis with increased an expression level of glial fibrillary acid protein (GFAP), which leads to overgrowth of outer Müller glial cell processes. (molvis.org)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)
the expression levels of protein associated with apoptosis and endoplasmic reticulum stress (ERS) of SKO-007 cells in various groups were detected by Western blotting method. (researchgate.net)
Objective: To investigate the effects of prion protein 106-126 peptide on inducing apoptosis in differentiated PC12 cells. (researchgate.net)
Methods: After differentiated by nerve growth factor (NGF), the PC12 cells were infected by prion protein 106-126 peptide. (researchgate.net)
Conclusion: Prion protein 106-126 peptide can induce apoptosis in differentiated PC12 cells and present cellular toxicity definitely. (researchgate.net)
One such protein is the cytoskeleton-associated protein 5 (CKAP5), widely expressed in a variety of cells to regulate the dynamics of microtubules in human cells. (medicalxpress.com)
FKBPL depletion significantly attenuated the pro-apoptotic protein, phospho-p53 (S46), in lung ADC H1975 cells. (jcancer.org)
UL16 is a protein that binds to the ligand NDG2D which is a surface receptor on NK cells and, when blocked, prevents the CMV infected cell from apoptotic signaling to NK cells to destroy it. (loinc.org)
Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)

A significant decrease (0.05-0.5 μM) was observed in the cell viability and IC 50 dosage was fixed as 0.25 μM after 24 h of treatment. (degruyter.com)
Results: Trametinib and SCH772984, alone and in combination with ribociclib, significantly reduced NET cell viability and arrested NET cells at the G1 phase of the cell cycle in all three cell lines tested. (uni-muenchen.de)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
Cell viability was determined by using the MTT assay and the morphological changes were observed. (researchgate.net)
A) Cell viability at 72 hours and (B) 6 days post-siCKAP5 treatment as measured by methoxynitrosulfophenyl-tetrazolium carboxanilide (XTT). (medicalxpress.com)
Values represent average % cell viability compared to cells treated with an equivalent concentration of siControl LNP. (medicalxpress.com)
NCAM polysialylation during adherence transitions: live cell monitoring using an antibody-mimetic EGFP-endosialidase and the viability dye DRAQ7. (cardiff.ac.uk)
The combination of NV651 and cisplatin, a DNA damage reagent, resulted in a synergistic effect in cell viability, and a significant increase on cell death in HCC cell lines. (lu.se)

It is also responsible for inhibition of vascular endothelial growth factors receptors, plateletderived growth factor receptors, and stem cell factor receptor [ 8 , 9 ]. (hindawi.com)
Inhibition of VCP either by genetic depletion or the pharmacologic inhibitor CB-5083 increased ubiquitination and degradation of p53-R273H, leading to cell death. (aacrjournals.org)
Conclusions: MEK and ERK inhibition causes antiproliferative effects in human NET cell lines in vitro. (uni-muenchen.de)
Therefore, we used OEO and its main component, thymol, to explore their effects on cell growth inhibition and anticancer activity. (irost.org)
consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
Inhibition of p53 by pifithrin-α significantly reduced the levels of PUMA, Bax, and PTEN but restored AKT phosphorylation in SiHa cells exposed to sesamin. (medsci.org)
In preclinical studies, vimseltinib durably suppressed CSF1R activity in vitro and in vivo, depleted macrophages and other CSF1R-dependent cells, and resulted in inhibition of tumor growth and bone degradation in mouse cancer models. (bvsalud.org)
Translationally, in a phase I clinical study, vimseltinib treatment led to modulation of biomarkers of CSF1R inhibition and reduction in tumor burden in TGCT patients. (bvsalud.org)
Pharmacological inhibition of polysialyltransferase ST8SiaII modulates tumour cell migration. (cardiff.ac.uk)
We could confirm the increased polyploidy upon Mps1 inhibition in neuroblastoma cell lines and PDX model, leading to mitotic catastrophe activating the caspase-dependent mitochondrial apoptotic pathway. (lu.se)

In mammalian cells the DDR is a network of pathways made up of proteins that function as either kinases, or and mediator/adaptors that recruit the kinases to their phosphorylation targets, these factors work together to detect DNA damage, and signal the repair mechanism as well as activating cell cycle checkpoints. (wikipedia.org)
T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). (cellsignal.com)
Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). (cellsignal.com)
Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
Cell mitosis is an attractive target to effectively treat a variety of cancer forms, where microtubule-associated proteins (MAPs) assist cells to maintain the stability of cell dynamics. (medicalxpress.com)
Our results indicate that several dozens of proteins for each cell type, are PE-lipoxidated in HT-22, MLE, and H9c2 cells and M2 macrophages after they were induced to undergo ferroptosis. (cdc.gov)
Pretreatment of cells with the strong nucleophile, 2-mercaptoethanol, prevented the formation of PE-lipoxidated proteins and blocked ferroptotic death. (cdc.gov)

The MTT assay was used to examine the cytotoxic effects of fungal taxol against the human liver cancer cell line HepG2. (degruyter.com)
One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
This study was aimed to specify the cytotoxic effect of C. sativa -derived extracts on colon cancer cells and adenomatous polyps by identification of active compound(s) and characterization of their interaction. (nih.gov)
Ethanol extracts of C. sativa were analyzed by high-performance liquid chromatography and gas chromatograph/mass spectrometry and their cytotoxic activity was determined using alamarBlue-based assay (Resazurin) and tetrazolium dye-based assay (XTT) on cancer and normal colon cell lines and on dysplastic adenomatous polyp cells. (nih.gov)
The unheated cannabis extracts (C2F), fraction 7 (F7), and fraction 3 (F3) had cytotoxic activity on colon cancer cells, but reduced activity on normal colon cell lines. (nih.gov)
In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells. (tubitak.gov.tr)
The purpose of this study was to evaluate the phenolic composition and antioxidant activity of dimethyl sulfoxide extract of M. nigra (DEM) and to investigate, for the first time, the probable cytotoxic effect in human prostate adenocarcinoma (PC- 3) cells together with the mechanism involved. (ktu.edu.tr)
The cytotoxic effect of DEM on PC-3 cells was revealed using the MTT assay. (ktu.edu.tr)
Mechanisms involved in the cytotoxic effect of DEM on PC-3 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, while caspase activity was investigated using luminometric analysis. (ktu.edu.tr)

In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
The present study demonstrated that genistein suppressed the migration of colon cancer cells by reversal the EMT via suppressing the Notch1/NF-κB/slug/E-cadherin pathway. (biomedcentral.com)
After polymers produced by 2-5OAS activate RNase L, RNase L begins to fragment mRNA and upregulates an apoptotic pathway involving caspases. (phoenixrising.me)
A second apoptotic pathway induced via PKR activation acts by blocking mRNA translation (via EIF-2a), and by activating transcription (NF-kB) and regulatory factors (IRF-1). (phoenixrising.me)

The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)
Eribulin exerts its effects via a tubulin-based antimitotic mechanism leading to G2/M cell-cycle block, disruption of mitotic spindles, and, ultimately, apoptotic cell death after prolonged mitotic blockage. (shu.edu)
Treatment with the novel cyclophilin inhibitor NV651, presented a potent antiproliferative effect in HCC cell lines via cell cycle perturbations arresting cells in the mitotic phase. (lu.se)
Targeting the cell cycle has been proposed as a therapeutic strategy in cancer treatment with special interest in the mitotic phase. (lu.se)

Based on the significant antibacterial activity, the sorting of bacterial cells against the compounds (MID6 and MID9) was analyzed by flow cytometry. (degruyter.com)

Measurement of apoptotic markers in tumors can be directly correlated with the cell cycle phase using flow cytometry (FCM). (edu.au)
Consistently, ablation of VCP markedly retarded growth of cultured PDAC cells and xenograft PDAC tumors. (aacrjournals.org)
In the rare disease tenosynovial giant cell tumor (TGCT), aberrant CSF1 expression and production driven by a gene translocation leads to the recruitment and growth of tumors formed by CSF1R-dependent inflammatory cells. (bvsalud.org)

We provide new evidence that a simple 2D flow focusing on a chip is sufficient to measure cellular DNA content in live tumor cells using a far-red DNA probe DRAQ5. (edu.au)
The data show that μFCM can provide a viable novel alternative to conventional FCM for multiparameter detection of caspase activation and dissipation of mitochondrial inner membrane potential (ΔΨm) in relation to DNA content (cell cycle phase) in live tumor cells. (edu.au)
The main goal of RT consists in delivering a precise dose of radiation in a target volume, such as tumor, promoting the tumor cells eradication with as minimal damage as possible in surrounding normal tissues ( 13 ). (frontiersin.org)
Objectives Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. (immune-source.com)
Cell cycle analysis showed an accumulation of tumor cells predominantly in 511296-88-1 manufacture G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. (immune-source.com)
Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. (immune-source.com)

Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)
0.01, Student t test), whereas no significant changes were observed in the cell cycle progression. (conicet.gov.ar)
L-DOPA: 30.30, ×2) In addition, we analyzed the effects of L-DOPA on anterior pituitary cell cycle progression. (conicet.gov.ar)
Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
Epithelial mesenchymal transition (EMT) is an important process during tumor progression which affects critical steps of morphogenesis by interconverting epithelial cell types into cells with mesenchymal attributes [ 11 ]. (biomedcentral.com)
Using this stochastic model with a simulation program, we derived the equilibrium distribution of cell population and simulated the progression of the neurogenic cascade. (biomedcentral.com)
Time-lapse microscopy approaches to track cell cycle and lineage progression at the single-cell level. (cardiff.ac.uk)
Approval of palbociclib for initial endocrine-based therapy in postmenopausal women was based on the phase II trial (PALOMA-1) which measured median progression-free survival (PFS) duration. (medscape.com)

In addition, trametinib also caused subG1 events and apoptotic PARP cleavage in QGP1 and NCI-H727 cells. (uni-muenchen.de)
In Xenopus laevis, early embryonic development consists of twelve rapid cleavage cycles between DNA replication (S) and mitosis (M) without checkpoints or gap phases. (vt.edu)

However, existing mitosis-targeting chemotherapeutic agents do not discriminate between healthy and malignant cell lines, resulting in severe side-effects . (medicalxpress.com)
The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
The checkpoint original model represents the activity of Cdc25 phosphatase on the mitosis promoting factor (MPF) that leads the cell into mitosis. (vt.edu)
Similar to these drugs, eribulin is a microtubule inhibitor that induces apoptosis of cancer cells by stopping mitosis in the G2/M phase of the cell cycle . (shu.edu)

Phase contrast microscopy evidenced some specific morphological modifications indicating apoptosis allied cytotoxicity of piperine like apoptotic bodies, condensed nucleus and membrane blabbing. (ijpsonline.com)
Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
The melatonin treatment resulted in increased caspase-3 activity and reduced the mitochondrial membrane potential in the 5RP7 cells for 48 h. (tubitak.gov.tr)
The existence of apoptotic bodies, cell membrane blebbing and chromatin condensation indicated the hallmark of apoptosis. (um.edu.my)
DEM arrested the cell cycle of PC-3 cells at the G1 phase, induced apoptosis via increased caspase activity and reduced mitochondrial membrane potential. (ktu.edu.tr)

Antiproliferative and apoptotic effect o. (ktu.edu.tr)

In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
One goal of this work is to understand the developmental role and regulation of checkpoint signaling pathways due to its monitoring of DNA integrity within the cell. (vt.edu)
To address the multiple potential pathways of drug resistance in GIST, the KIT/PDGFRA inhibitor ripretinib was combined with MEK inhibitors in cell lines and mouse models. (bvsalud.org)
this suggests that the novel independent signaling pathways KPNA3-TGF-β-GATA3-HAS2/E-cadherin and KPNA3-AKT-HAS2/E-cadherin are involved in the EMT-mediated progress of TNBC MDA-MB-231 cells. (bmbreports.org)
In this study, it was found that KPNA3 regulates numerous EMT-related genes and induces the EMT process via at least two independent signaling pathways in the highly invasive triple-negative breast cancer (TNBC) cell line MDA-MB-231. (bmbreports.org)
The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Several pathways involved in cell cycle and DNA repair were affected upon NV651 treatment. (lu.se)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
Objective: To investigate the induction of tanshinone [1 A (Tan fl A) on the differentiation of human placenta-derived mesenchymal stem cells (hPDMSCs) into cardiomyocytes, and to provide an experimental basis for Tan IT A as a cardiomyocytc differentiation inducer. (researchgate.net)
Epithelial-mesenchymal transition (EMT) is a key cellular process in which immotile epithelial cells transform into mesenchymal cells through cell polarity loss, cell-cell junction disassembly, and extracellular matrix (ECM) alteration. (bmbreports.org)
While knowledge of genistein in regulating epithelial mesenchymal transition (EMT) of colon cancer cells is unknown. (biomedcentral.com)
Genistein was found can induce the reversal of EMT in prostate cancer cells by an upregulated expression of epithelial marker E-cadherin and the loss of expression of mesenchymal marker vimentin [ 22 ]. (biomedcentral.com)

E) Confocal microscopic images of Cy5siRNA-labeled LNP internalization in SKOV3, NAR, and A549 cells. (medicalxpress.com)

Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
3- 5 Therefore, during severe hypoxia or anoxia, the cell initiates a cascade of events that leads to apoptotic cell death, thereby preventing the accumulation of cells with hypoxia induced mutations. (bmj.com)
At this point, the embryo initiates transcription, acquires gap phases between S and M phases, and establishes a functional apoptotic program. (vt.edu)

Another means of characterizing the unreplicated DNA checkpoint is through the use of mathematical modeling of the checkpoint-signaling cascade of the cell cycle. (vt.edu)
The involvement of p53 in the apoptotic cascade was assessed by a specific inhibitor. (medsci.org)
the neuroblast stage has the highest temporal variance within the cell types of the neurogenic cascade, while the apoptotic stage is short. (biomedcentral.com)

Apoptotic was detected by morphological changes, DNA electrophoresis and flow cytometry. (researchgate.net)
Thus, adult neurogenesis represents another means, apart from molecular, synaptic, or morphological changes of an individual cell, to alter the functional circuitry depending on the demand. (biomedcentral.com)

Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)

In cultured anterior pituitary cells from ovariectomized rats, L-DOPA (10-5 M, 6h) decreased the apoptotic index (determined by TUNEL assay. (conicet.gov.ar)
Flow cytometry, caspase-3 activity assay in treated monolayers/spheroids and also fluorescence staining and DNA fragmentation in treated monolayers demonstrated apoptotic death mode. (irost.org)
Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)
Cell migration and invasion was monitored by transwell chambers assay. (ijpsonline.com)
Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
Annexin V Assay and fluorescence-activated cell sorting (FACS) were used to determine apoptosis and cell cycle, and RNA sequencing was used to determine gene expression. (nih.gov)

Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
Cell migration as well as cell invasive ability of MCF-7 cells was reduced to minimum by the application of piperine in dose dependent-manner. (ijpsonline.com)
Dose-dependent response of CKAP5 knockdown across various cancer cell lines. (medicalxpress.com)

In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
In recent years, we focused on investigating the anti-carcinogenic properties of total Astragalus saponins (AST) in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
To investigate the effects and mechanisms of genistein on EMT of colon cancer cells, HT-29 cells were used and treated by genistein and TNF-α in this paper. (biomedcentral.com)
Genistein inhibited cell migration at 200 μmol/L. Genistein reversed the EMT of colon cancer cells by upregulation of E-cadherin and downregulation of N-cadherin, accompanied by the suppression of EMT related makers, such as Snail2/slug, ZEB1, ZEB2, FOXC1, FOXC2 and TWIST1. (biomedcentral.com)
In vitro data has shown that GEN can inhibit the growth of several colon cancer cells [ 8 ], while its particular effects on cancer cells and the mechanisms involved remain unknown [ 9 , 10 ]. (biomedcentral.com)
However, current knowledge of GEN in regulating EMT of colon cancer cells is limited, and more detailed investigations of its function and mechanism are required. (biomedcentral.com)

Muse flow cytometric analysis was performed for assessing of different cell cycle phases. (ijpsonline.com)
Cytometric routes to single cell transcriptomics. (cardiff.ac.uk)

This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)
Adding lunasin to IL-12- or IL-2-stimulated NK cells demonstrated synergistic effects in the induction of IFNG and GZMB involved in cytotoxicity. (weeksmd.com)
It is considered that the induction of apoptotic cell death and reversal of EMT are promising emerging strategy for prevention and treatment of cancer [ 20 , 21 ]. (biomedcentral.com)
Increase of Annexin V-FITC bound to phosphatidylserine confirmed the apoptosis induction in the cells. (um.edu.my)

This work describes development and proof-of-concept validation of a portable and user-friendly microfluidic flow cytometer (μFCM) that can perform multivariate real time analysis on live cells using sampling volumes as small as 10 microliters. (edu.au)
Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis. (researchgate.net)
Results: The doppler spectrum of normal fetal DV blood flow showed the same three-phase waves, SV was increased with the increase of gestational age (F=27. (researchgate.net)
Micro-community cytometry: sensing changes in cell health and glycoconjugate expression by imaging and flow cytometry. (cardiff.ac.uk)

Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)

EMT was determined by cell invasion assays using a transwell chamber and the expression changes of EMT-related markers were confirmed by RT-PCR, Western blotting, and immunofluorescence staining. (biomedcentral.com)

However, the co-immunoreactivity of nestin and GS, a marker of mature functional Müller cells, could be observable only from the seventh day. (molvis.org)
However, despite a significant functional relevance of this form of whole-cell plasticity, little is known about the processes that regulate it. (biomedcentral.com)

Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). (cellsignal.com)
These findings suggest that formononetin inhibits angiogenesis and tumor cell invasion, and thus support its use in the treatment of advanced and metastatic colon cancers. (spandidos-publications.com)
Eribulin inhibits the growth phase of microtubules without affecting the shortening phase and sequesters tubulin into nonproductive aggregates. (shu.edu)
Our findings showed that plagioneurin B successfully inhibits the growth of CAOV-3 cells at IC 50 of 0.62 µM. (um.edu.my)

MID9 showed a moderate level of cell death compared to the control. (degruyter.com)
7 Severe hypoxia in the presence of energy stimulates cells to undergo apoptosis, whereas oxygen levels above 0.5% prevent cell death. (bmj.com)
These findings show that melatonin induces cell death and apoptosis in H-ras oncogene transformed 5RP7 cells. (tubitak.gov.tr)
Apoptosis, which is a major way of programmed cell death, has been known to all plays an important role in the regulation of tissue development and homeostasis [ 17 ]. (biomedcentral.com)
The apoptotic cell death was determined by fluorescent staining with acridine orange and ethidium bromide, which confirmed the presence of apoptotic cells. (bgu.ac.il)
The result also indicates that the apoptotic cell death in the cancer cell might be triggered by the process of ROS generation with the complexes. (bgu.ac.il)
Cyclophilin overexpression is a common event in fibrotic tissues playing a key role in different stages of the fibrotic process, including inflammation, hepatocyte death, and activation of the hepatic stellate cells leading to increased collagen production. (lu.se)
Ferroptosis is an iron dependent form of cell death, that is triggered by the discoordination of iron, lipids, and thiols. (cdc.gov)
Its unique signature that distinguishes it from other forms of cell death is the formation and accumulation of lipid hydroperoxides, particularly oxidized forms of polyunsaturated phosphatidylethanolamines (PEs), which drives cell death. (cdc.gov)
2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)

This gene thus plays a role in both cell-cycle and epigenetic regulation. (cancerindex.org)
The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)

Acetogenin group has attracted worldwide attention in the past few years due their biological abilities as inhibitors for several types of tumour cells. (um.edu.my)

Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
In this study we investigated the effects of the MEK inhibitor trametinib, the ERK inhibitor SCH772984 and the CDK4/6 inhibitor ribociclib in human neuroendocrine tumor cell lines BON1, QGP1 and NCI-H727 in vitro. (uni-muenchen.de)
Objectives: This study assessed the antitumoral activity of the MEK inhibitor trametinib (TMT212) and the ERK1/2 inhibitor SCH772984, alone and in combination with the CDK4/6 inhibitor ribociclib (LEE011) in human neuroendocrine tumor (NET) cell lines in vitro. (uni-muenchen.de)

G1MTC, G1 phase microtubule checkpoint. (shu.edu)

Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. (hindawi.com)
This investigation aims to analyse the in vitro effects of cisplatin and sunitinib malate in isolation and in combination, on one human nonmuscle invasive urinary bladder-cancer cell line (5637) and on two human muscle-invasive urinary bladder-cancer cell lines (T24 and HT1376). (hindawi.com)
The study was performed on the 5637, T24, and HT1376 urinary bladder-cancer cell lines. (hindawi.com)
Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
Further, application of piperine on cancer cell colonies remarkably reduced the number of cell colonies to minimum. (ijpsonline.com)
In this work, Chatterjee and the team screened the effect of CKAP5 silencing in solid cancer cell lines and in normal non-cancer epithelial cell lines as a negative control. (medicalxpress.com)
Increasing evidence emphasizes a critical role of EMT endowing the incipient cancer cell with invasive and metastatic properties [ 16 ]. (biomedcentral.com)

In bottom line, we confirmed that autophagy brought about by genotype VII NDV infections was important for virus-like replication, NDV-induced apoptosis, and cell survival in buy 480-11-5 chicken cells and tissues. (immune-source.com)

In cells stimulated to undergo ferroptosis we identified PE-truncated species with sn-2 truncations ranging from 5 to 9 carbons. (cdc.gov)

Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)

Colony-stimulating factor 1 receptor (CSF1R)-dependent macrophages and other inflammatory cells can suppress the adaptive immune system in cancer and contribute to angiogenesis, tumor growth, and metastasis. (bvsalud.org)
Once apoptotic and/or inflammatory activity occurs, the native RNase L is broken up and the 37-kDa fragment is generated. (phoenixrising.me)

Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
It is designed to treat micrometastatic disease (or breast cancer cells that have escaped the breast and regional lymph nodes but which have not yet had an established identifiable metastasis). (medscape.com)

Knock out MDC1 mice cells and silenced human cells were radiosensitive, failed to initiate Intra-S phase and G2/M checkpoints, failed to produce ionizing radiation-induced foci had poor phosphorylation by the DRR kinases (ATM, CHK1, CHK2), defects in homologous recombination. (wikipedia.org)
During the cell cycle, there are two main checkpoints that regulate entrance into S and M phases. (vt.edu)
Two MTD-induced cell cycle checkpoints. (shu.edu)

The DDR of mammalian cells is made up of kinases, and mediator/adaptors factors. (wikipedia.org)
Mammalian cells have developed a range of adaptations to survive acute and even prolonged hypoxia. (bmj.com)
The process of autophagy in mammalian cells involves six principal actions: initiation, nucleation, elongation, closure, maturation, buy 480-11-5 and degradation [27]. (immune-source.com)

Chk1 plays a critical but not fully understood role in cell cycle remodeling and early embryonic development. (vt.edu)
Melatonin was not toxic in NIH/3T3 primary mouse embryonic fibroblast cells at low doses for 24 and 48 h. (tubitak.gov.tr)

On the other hand, ATF-3 is rapidly induced in cells treated with growth stimulators such as serum and growth factors [ 20 ]. (hindawi.com)
Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
Methods: The human multiple myeloma SKO-007 cells in logarithm growth phase were selected and randomly divided into control group, LDM group, BZM group, and BZM combined with LDM group. (researchgate.net)
Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. (tubitak.gov.tr)
exhibited a growth inhibitory effect on both cell lines. (immune-source.com)

The tumor size and the number of proliferating cells were reduced in the tumor tissues obtained from the formononetin-treated group. (spandidos-publications.com)
The scope of the special issue is to summarize and enlarge the knowledge in signalling processes and networks in diverse cells and tissues. (mdpi.com)
In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. (immune-source.com)

BC comprises of variant subtypes and those subtypes arise from basal progenitor or luminal progenitor cells undergoing different genetic mutations[ 2 ]. (ijpsonline.com)

Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
Tumor necrosis factor-α (TNF-α) has been considered stimulated the EMT in several kinds of cancer cells which is a function that contrasts with its more established role in inducing apoptosis [ 7 , 12 , 13 ]. (biomedcentral.com)
Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)

GEN was also suggested can inhibit cell migration and invasion in both AsPC-1 and Notch-1-over-expressed AsPC-1 cells as Notch-1 could play a key role in the regulation of EMT [ 23 ]. (biomedcentral.com)

In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
Its therapeutic effects on urinary bladder cancer have already been assessed in two clinical studies of phase II cancers and showed clinical benefits [ 10 , 11 ]. (hindawi.com)
The reduction in tumor volume as well as pro-apoptotic and anti-proliferative effects in HT-29 nude mice xenograft are comparable to those produced by 5-FU. (spandidos-publications.com)
In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
With the goal of further improving cytokine-based immunotherapy, we examined the effects of a soybean peptide called lunasin that synergistically works with cytokines on natural killer (NK) cells. (weeksmd.com)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)

Neutropenia: Monitor peripheral blood cell counts and adjust dose as appropriate. (drugs.com)
Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
A complete blood cell count (CBC) with differential should be obtained, including examination of the peripheral blood smear if the differential is abnormal. (medscape.com)

Anatomy25

Organisms10

Diseases9

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Psychiatry and Psychology1

Phenomena and Processes76

Disciplines and Occupations1

Information Science5

Health Care5

Reduced apoptosis2

Proliferation27

Anti-apoptotic4

Induce cell3

Arrest11

Protein26

Viability8

Inhibition11

Proteins8

Cytotoxic9

Pathway4

Mitotic4

Analyzed by flow cytometry1

Tumors3

Tumor cells6

Progression9

Cleavage2

Mitosis4

Membrane5

Antiproliferative1

Pathways9

Mesenchymal5

A5491

Initiates3

Cascade3

Morphological changes2

PARP1

Assay6

Dose-dependent3

Colon cancer cells8

Cytometric2

Induction4

Flow4

Tumours1

Assays1

Functional2

Inhibits4

Death10

Gene3

Inhibitors1

Inhibitor3

Checkpoint1

Cancer cell8

Replication1

Undergo1

Differentiation1

Inflammatory2

Metastasis3

Checkpoints3

Mammalian cells3

Embryonic2

Growth5

Tissues3

Progenitor1

Necrosis3

Migration and invasion1

Effects6

Peripheral3