Apoptosis of human umbilical vein endothelial cells. Medical search. Frequent questions

However, the effects of NAC on LPS‑induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the associated mechanisms are not well characterized. (spandidos-publications.com)
The present study explored the effect of NAC on LPS‑induced apoptosis of HUVECs and determined the participation of the p38 mitogen‑activated protein kinase (MAPK) pathway in the process of apoptosis. (spandidos-publications.com)
The results of CCK‑8 and flow cytometric analyses showed that pretreatment of HUVECs with NAC or p38MAPK inhibitor (SB203580) attenuated LPS‑induced decrease in cell viability and increase in cell apoptosis. (spandidos-publications.com)
The results demonstrated that NAC pretreatment alleviated LPS‑induced apoptosis and inhibition of NO production in HUVECs. (spandidos-publications.com)
Human umbilical vein endothelial cells (HUVECs) were treated with ginkgolide B. The expression of PXR, CYP3A4, MDR1, VCAM-1, E-selectin and caspase-3 were quantified with qRT-PCR and Western blot analysis. (greenmedinfo.com)
Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
Gain of function and loss of function experiments showed that PDCD4 induced turnover (proliferation and apoptosis) of HUVECs. (plos.org)
PDCD4 induces turnover (proliferation and apoptosis) of HUVECs. (plos.org)
Expression of chemoresistance molecules was evaluated using flow cytometry in U87MG and human umbilical vein endothelial cells (HUVECs). (biomedcentral.com)
Human umbilical vein endothelial cells (HUVECs) were incubated with Met, Lira, or their combination in this study. (ac.ir)
This study showed that combination therapy with Lira and Met could effectively reduce cell proliferation, induce apoptosis, and inhibit cell migration in the HUVECs. (ac.ir)
however, very few studies have investigated X-ray-related changes in telomere length and the telomerase activity in normal human cells, such as umbilical vein endothelial cells (HUVECs). (telomerescience.com)
Therefore, a 0.5-Gy dose of IR induces persistent apoptosis leading to an apparent growth arrest of the normal HUVECs. (telomerescience.com)
In this study, we profiled the gene expression of human umbilical vein endothelial cells (HUVECs) exposed to different concentrations of TCDD by high-throughput sequencing. (elsevierpure.com)
In vitro inhibition of vascular endothelial growth factor induced proliferation of human umbilical vein endothelial cells (HUVECs) by the glycoprotein further supports its antiangiogenic activity. (uni-mysore.ac.in)

The aim of this study was to assess whether activation of nrf2 by sulforaphane in human microvascular endothelial cells prevents metabolic dysfunction in hyperglycemia. (diabetesjournals.org)
The functional mechanism of circular RNA (circRNA) HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (circ_HECW2, hsa_circ_0057583) in ox-LDL-treated human cardiac microvascular endothelial cells (hCMECs) is still unclear. (semcs.net)

Since endothelial dysfunction precedes overt atherosclerosis, investigating the ability of drugs to activate cytoprotective signalling pathways that prevent or reverse endothelial dysfunction is an attractive research strategy. (bmj.com)
CONCLUSIONS- We conclude that activation of nrf2 may prevent biochemical dysfunction and related functional responses of endothelial cells induced by hyperglycemia in which increased expression of transketolase has a pivotal role. (diabetesjournals.org)
The mechanism that contributes to NK cell dysfunction in CHB needs further elucidation. (bvsalud.org)
Dysfunction of vascular Endothelial Cells (ECs) has regarded as a high risk factor for the Atherosclerosis (AS) development [ 1 , 2 ]. (biomedres.info)
Oxidized low-density lipoprotein (ox-LDL) is a well-known culprit of atherogenesis, which causes a series of aberrant initial changes in vascular smooth muscle cells (SMCs) including lipid homeostasis and deposition, dysfunction of Ca 2+ homeostasis, and apoptosis [ 1 , 2 ]. (oncotarget.com)
Apoptosis of endothelial cells could be an initial manifestation leading to endothelial dysfunction and subsequent plaque development. (uni-frankfurt.de)

A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)
The present study investigated how lysoPC induces apoptosis in cultured human coronary artery SMCs using cell viability assay, flow cytometry, confocal microscopy, and molecular biological approaches. (oncotarget.com)
Lysophosphatidylcholine (lysoPC) is a major component of ox-LDL [ 3 , 4 ], and induces apoptosis of vascular endothelial cells and SMCs [ 5 - 7 ]. (oncotarget.com)

The rate of apoptosis was determined using flow cytometry. (spandidos-publications.com)
Cell apoptosis was analyzed with flow cytometry. (greenmedinfo.com)
Cell proliferation and apoptosis were analyzed by 5-ethynyl-2'-deoxyuridine (EdU) assay, cell counting kit-8 (CCK8) assay, and flow cytometry, respectively. (semcs.net)

Furthermore DATS treatment inhibited angiogenesis in human being umbilical vein endothelial cells (20). (immune-source.com)
Role of metformin in suppressing 1, 2-dimethylhydrazine-induced colon cancer in diabetic and non-diabetic mice: effect on tumor angiogenesis and cell proliferation. (ac.ir)
Exenatide suppresses 1, 2-dimethylhydrazine-induced colon cancer in diabetic mice: Effect on tumor angiogenesis and cell proliferation. (ac.ir)
Tube formation assay was performed to analyze the angiogenesis of cells. (semcs.net)
N-terminal sequence of the high performance liquid chromatography (HPLC) pure glycoprotein showed sequence homology to an extent of 40-50 with known angiogenesis inhibitor and apoptosis-inducing protein from C. elegans and G. gallus respectively. (uni-mysore.ac.in)
Together, these data indicate that HBO1 promotes migratory tip cell behaviour during sprouting angiogenesis. (silverchair.com)
In this review, we highlight recent evidence related to the molecular mechanism of lncRNAs in growth, survival, invasion, metastasis, angiogenesis and apoptosis of PC cells, and discuss the potential clinical application of lncRNAs to the diagnosis, treatment and prognostic prediction of PC. (oncotarget.com)

RESEARCH DESIGN AND METHODS- Human microvascular HMEC-1 endothelial cells were incubated in low and high glucose concentrations (5 and 30 mmol/l, respectively), and activation of nrf2 was assessed by nuclear translocation. (diabetesjournals.org)
Most farm animals possess Paneth cells in their small intestine, as identified by various staining methods, and Paneth cells of various livestock species exhibit noticeable differences in cell shape, granule number, and intestinal distribution. (biomedcentral.com)
In the current work, we used well-known methods (ToppGene and Endeavour), as well as methods previously developed by us, to prioritize genes involved in endothelial apoptosis and to find potential participants of molecular-genetic mechanisms of lymphedema among them. (biomedcentral.com)
Original methods of prioritization took into account the overrepresented Gene Ontology biological processes, the centrality of vertices in the associative gene network, describing the interactions of endothelial apoptosis genes with genes associated with lymphedema, and the association of the analyzed genes with diseases that are comorbid to lymphedema. (biomedcentral.com)
We apply molecular toxicology methods such as in vitro human cell culture assays. (uagm.edu)

In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). (geneticsmr.com)
particularly, the effect of miR-195-5p modulation seemed to affect cell cycle, while miR-107 seemed to be implicated in the inhibition of cells migration. (biomedcentral.com)
Metformin blocks migration and invasion of tumour cells by inhibition of matrix metalloproteinase‐9 activation through a calcium and protein kinase Cα‐dependent pathway: Phorbol‐12‐myristate‐13‐acetate‐induced/extracellular signal‐regulated kinase/activator protein‐1. (ac.ir)
Hence, inhibition of ox-LDL induced ECs apoptosis may suggest new therapies in the AS treatment. (biomedres.info)
The mechanism of antiangiogenesis involved inhibition of translocation of nuclear factor kappa B to the nucleus resulting in decreased expression of vascular endothelial growth factor gene as is demonstrated by our results on quantification of vascular endothelial growth factor levels in the glycoprotein treated tumor bearing mice. (uni-mysore.ac.in)
1 - 4 Multiple mechanisms may be involved in its potent antitumor effect, including induction of apoptosis, cell cycle arrest, telomerase inhibition, antiangiogenesis activity, and an antimetastasis effect. (dovepress.com)

We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
The intestinal epithelium consisting of a single layer of epithelial cells plays an important role in absorbing the digested dietary nutrients and separating the large amounts of microorganisms in the intestinal lumens from the lamina propria of intestine [ 7 ]. (biomedcentral.com)
As a type of long-lived epithelial cells, Paneth cells possess approximately one-month lifespan in small intestine [ 11 ]. (biomedcentral.com)
Increase inflammation promotes apoptosis in the epithelial cells, endothelial cells, and airways, that resulting Emphysema. (jeoct.com)
The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)

The expression of caspase‑3, Bax, Bcl‑2, phosphorylated (p)‑p38MAPK/total (t‑)p38MAPK and p‑endothelial e nitric oxide synthase (eNOS)/t‑eNOS proteins were determined by western blotting. (spandidos-publications.com)
Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)

Heparin fails to inhibit the proliferation of human vascular smooth muscle cells in the presence of human serum. (shengsci.com)
The proliferation of vascular smooth muscle cells (VSMC) plays a significant part in both the developing atherosclerotic lesion and in restenosis. (shengsci.com)

Cytotoxicity was not related to apoptosis. (biomedcentral.com)
ILT2 blockade was shown to increase the cytotoxicity and IFN-Î³ production of CD56dimNK cells in some CHB patients. (bvsalud.org)
In this context, human tumor and normal cells are treated in culture and their cytotoxicity and cell death mechanism (apoptosis and autophagy) are determined. (uagm.edu)
In recent studies, morroniside showed the protective actions against the cytotoxicity produced by exposure to H 2 O 2 in human SH-SY5Y cells [ 5 , 6 ]. (ijpsonline.com)
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) tests demonstrated that the GA-mPEG 2000 micelle formulation had obvious cytotoxicity to tumor cells and human umbilical vein endothelial cells. (dovepress.com)

Apoptosis refers to the self-regulated and orderly death of cells controlled by genes in order to maintain the stability of internal environment ( 8 ). (spandidos-publications.com)
Studies of human umbilical vein endothelial cells (HUVEC) and arterial endothelial cells (HAEC) showed that therapeutically relevant concentrations of MTX phosphorylate AMPKα Thr172 , and induce cytoprotective genes including manganese superoxide dismutase (MnSOD) and haem oxygenase-1 (HO-1). (bmj.com)
Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
However, a study of the relationship between genes associated with lymphedema and genes involved in endothelial apoptosis, in the associative gene network was not previously conducted. (biomedcentral.com)
An assessment of the quality of prioritization was performed using criteria, which involved an analysis of the enrichment of the top-most priority genes by genes, which are known to have simultaneous interactions with lymphedema and endothelial cell apoptosis, as well as by genes differentially expressed in murine model of lymphedema. (biomedcentral.com)
Analysis of interactions of these genes in the associative gene network of lymphedema can improve understanding of mechanisms of interaction between endothelial apoptosis and lymphangiogenesis, and shed light on the role of disturbance of these processes in the development of edema, chronic inflammation and connective tissue transformation during the progression of the disease. (biomedcentral.com)
KEGG pathway analysis showed that many genes in the signaling pathways of vascular smooth muscle contraction and apoptosis were altered distinctly. (elsevierpure.com)
These DE-miRNAs, hub genes and pathway were proved in arecoline-treated HepG2 cells. (biomedcentral.com)
Finally, chromatin immunoprecipitation and sequencing in human umbilical vein ECs shows that H3K14ac modifications are enriched at key vascular genes, such as PECAM1 . (silverchair.com)
The Human Genome Project has revealed that protein-coding genes represent less than 2% of the total genome sequence [ 7 ], and the remaining greater portion of human genomes are regarded as "junk DNAs", for they do not encode any protein. (oncotarget.com)

Treatment of HCAECs to ox-LDL (150 μg/ml) decreased cell viability, stimulated apoptosis and enhanced caspase-3 activity. (biomedres.info)
Furthermore, the treatment with QL (0.5 μg/ml) significantly increased ox-LDL (150 μg/ml) decreased cell viability. (biomedres.info)
death and decreased cell viability under hypoxia (P0.021). (shsmu.edu.cn)

In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. (geneticsmr.com)
After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK-8) was used to detect growth inhibiting effects. (geneticsmr.com)
PYM could obviously inhibit the proliferation and promote the apoptosis of HUVEC. (geneticsmr.com)
PYM might induce HUVEC apoptosis through the endoplasmic reticulum stress pathway. (geneticsmr.com)
Human umbilical vein endothelial cell (HUVEC) apoptosis was quantified after EV exposure. (bvsalud.org)

Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
The only stimulant that enhanced PAC paracrine angiogenic activity was atorvastatin, which also had ability to stabilize endothelial tubes in vitro . (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
Aim of this study was to evaluate possible in vitro cytotoxic effects of two new-generation antiepileptic drugs on a human glioma cell line U87MG. (biomedcentral.com)
We used VES and FBZ, at low concentrations, singly and in combination, to test their inhibitory effects on proliferation of human and mouse prostate cancer cells in vitro. (researchgate.net)
To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy, the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized, and clinical safety and efficacy also need to be proven. (nature.com)
We found that the expanded cells possessed significantly improved immune effector functions, including proliferation, differentiation, and cancer cell killing, both in vitro and in the humanized mouse model. (nature.com)
We implement in-vitro molecular toxicology techniques applying human cancer cells in cultures. (uagm.edu)
A key aspect of such determination is identifying the capacity of these compounds to inhibit the growth of cancer cells in vitro. (uagm.edu)
Therefore, we investigated the in vitro effect of DHEA on the expression pattern of adhesion molecules of human endothelial cells and neutrophils. (biomedcentral.com)

To investigate the putative anti-proliferative effect, apoptosis assay, microRNA expression profile and study of cell cycle were performed. (biomedcentral.com)

2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
Although there has been a lot of studies and reviews on human and murine Paneth cells under intestinal homeostasis or disorders, little is known about Paneth cells in farm animals. (biomedcentral.com)

Furthermore, periodontitis has been shown to be associated with systemic cardiovascular disease ( 5 - 7 ), which suggests its close association with vascular endothelial cells. (spandidos-publications.com)
The number and function of proangiogenic cells are impaired in patients with diabetes or cardiovascular diseases. (hindawi.com)
QL showed the protective effects in the cardiovascular system via reducing myocardial apoptosis and cardiac fibrosis, thus protecting cardiac function. (biomedres.info)
But only little is known about apoptosis in cardiac and cardiovascular diseases. (uni-frankfurt.de)

Annexin V-FITC/propidium iodide stain was used to detect apoptosis, and western blot was used to detect the expression of glucose-related protein 78 (GPR78) and C/EBP homologous protein (CHOP) endoplasmic reticulum stress proteins. (geneticsmr.com)
Ginkgolide B (30-300μmol/L) did not change the mRNA and protein levels of PXR in the cells, but dose-dependently increased nuclear translocation of PXR protein. (greenmedinfo.com)
As far as LEV is concerned, in 2010 Bobustuc hypothesized that AED may modulate O-6 methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that has an important role in tumor cell resistance to alkylating agents, and LEV was reported as the most potent MGMT inhibitor among several AED [ 10 ]. (biomedcentral.com)
In conclusion the present study indicates that this DATS-induced apoptosis in prostate malignancy cells is usually mediated in part by suppression of XIAP protein expression and that XIAP signifies a viable biomarker of DATS response for future medical investigations. (immune-source.com)
The IAP family protein has emerged as crucial regulator of caspase-mediated apoptotic cell death by different stimuli (24-26). (immune-source.com)
13. Wu X, Li S, Xue P, Li Y. Liraglutide, a glucagon-like peptide-1 receptor agonist, facilitates osteogenic proliferation and differentiation in MC3T3-E1 cells through phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT), extracellular signal-related kinase (ERK)1/2, and cAMP/protein kinase A (PKA) signaling pathways involving β-catenin. (ac.ir)
In this study, we demonstrate that starvation of HeLa cells by incubation with Hank's buffered salt solution (HBSS) induced cell apoptosis, which was accompanied by the induction of reactive oxygen species (ROS) production and AMP-activated protein kinase (AMPK) phosphorylation. (tmu.edu.tw)
To investigate the effects and potential mechanisms of fatty acid binding protein 3 (FABP3) on cell survival under hypoxia. (shsmu.edu.cn)
Silencing TRPC1 or TRPC3 countered the lysoPC-induced increase of Ca 2+ influx and apoptosis, and the pro-apoptotic proteins Bax and cleaved caspase-3 and decrease of the anti-apoptotic protein Bcl-2 and the survival kinase pAkt. (oncotarget.com)
These results demonstrate the novel information that TRPC1/TRPC3 channels mediate lysoPC-induced Ca 2+ influx and apoptosis via activating the pro-apoptotic proteins Bax and cleaved caspase-3 and inhibiting the anti-apoptotic protein Bcl-2 and the survival kinase pAkt in human coronary artery SMCs, which implies that TRPC1/TRC3 channels may be the therapeutic target of lysoPC-induced disorders such as atherosclerosis. (oncotarget.com)
Although lncRNAs cannot encode any functional protein, they are involved in diverse biological processes, playing essential roles in maintaining cell growth, differentiation and proliferation [ 13 - 15 ]. (oncotarget.com)
Inhibitors of programmed cell death 1 (PD-1) protein and its ligands PD-L1 and PD-L2, are first-line agents in patients with metastatic urothelial carcinoma who are not candidates for platinum-containing chemotherapy, and second-line agents for those with disease progression despite cisplatin-based chemotherapy. (medscape.com)

Ca 2+ is a well-known second messenger that regulates a wide range of cell functions including excitation-contraction coupling, excitation-secretion coupling, gene transcription, cell growth, differentiation, apoptosis, membrane fusion, and ion channel activation [ 8 - 11 ]. (oncotarget.com)
CCN1 also induced Jag1 expression in hepatic stellate cells, whereupon they interacted with hepatic progenitor cells to promote their differentiation into cholangiocytes. (jci.org)
Studies in recent years have demonstrated that long non-coding RNAs (lncRNAs) have multiple biological functions in cell growth, differentiation and proliferation. (oncotarget.com)

Association of plasma visfatin levels with inflammation, atherosclerosis and acute coronary syndromes (ACS) in humans. (jeoct.com)
Therefore, the aim of this study was to investigate apoptosis in various cardiac diseases: in hyperlipidemia induced atherosclerosis, in acute rejected heart transplants, in ischemia and reperfusion as well as in chronic hypoxia. (uni-frankfurt.de)
To investigate apoptosis in hyperlipidemia induced atherosclerosis, genetically induced hyperlipidemia in Froxfield Heritable Hypercholesterolemic Rabbits (FFH, n=8) was compared with New Zealand White rabbits either fed with a cholesterol diet (H, n=8, 0,25% cholesterol, 3% coconut oil) or with a normal diet (control, n=5) for 12 weeks. (uni-frankfurt.de)

This study aim to evaluate the effect of QL on the cell viability, cell apoptosis and caspase-3 activity in oxidized LDL (oxLDL)-induced Human Coronary Artery Endothelial Cells (HCAECs). (biomedres.info)
In the present study, we used Human Coronary Artery Endothelial Cells (HCAECs) to investigate the protective effects of QL on ox-LDL induced cell injury. (biomedres.info)
We found that lysoPC reduced cell viability in human coronary artery SMCs by eliciting a remarkable Ca 2+ influx. (oncotarget.com)

The death receptor pathway plays an important role in cell apoptosis ( 12 ). (spandidos-publications.com)
The mitochondrial pathway is also referred to as the endogenous apoptosis pathway ( 15 ). (spandidos-publications.com)
TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)
TRIM13-induced NSCLC cell apoptosis was attenuated by a caspase-3 inhibitor Ac-DEVD-CHO, suggesting that TRIM13 induced cell apoptosis partially through a caspase-3-dependent pathway. (cancerindex.org)
Liraglutide promotes the angiogenic ability of human umbilical vein endothelial cells through the JAK2/STAT3 signaling pathway. (ac.ir)
Cell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathway. (jeoct.com)
A low concentration arecoline can induce the proliferation and migration of HepG2 cells, with the potential mechanism of action linked to high levels of exosomal miR-21 and miR-1267, activation of the PI3K-AKT pathway, upregulation of CDK1 and CCND1, and downregulation of RAF1. (biomedcentral.com)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells. (geneticsmr.com)
Mechanism of reversal of multidrug resistance by curcumin in human colorectal cancer cell line HCT-8/5-FU. (geneticsmr.com)
We've shown previously that garlic constituent diallyl trisulfide (DATS) inhibits growth of cultured and xenografted human prostate malignancy cells in association with apoptosis induction but the mechanism of cell death is not fully understood. (immune-source.com)
Our results show that chronic HBV infection increases the levels of the inhibitory receptor ILT2 on CD56dimNK cells and inhibits their functions, providing a new mechanism of NK-cell disability in CHB patients. (bvsalud.org)
Apoptosis was the likely mechanism for the observed effect. (researchgate.net)
Taken together, we for the first time demonstrated that a low-nutrient condition drives cancer cells to utilize glycolysis to produce ATP, and this increases the Warburg effect through a novel mechanism involving ROS/AMPK-dependent activation of PDK. (tmu.edu.tw)
Molecular mechanism of apoptosis-inducing factor regulated by FBXO22 and its role in cancer cell apoptosis [J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(4): 427-433. (shsmu.edu.cn)

Cell viability was assessed using the Cell Counting Kit‑8 (CCK‑8) assay. (spandidos-publications.com)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
The HCAECs viability was detected by cell proliferation assay and Lactate Dehydrogenase (LDH) release assay. (biomedres.info)
The HCAECs apoptosis was evaluated by the annexin V-FITC assay. (biomedres.info)
Cell migration assay and capillary-like tube formation assay on Matrigel were performed. (biomedres.info)
Neonatal rat ventricular myocytes were stimulated with recombinant human FABP3 proteins, and then the differences of cell viability and cell death between groups were verifiedtrypan blue assay and MTT assay. (shsmu.edu.cn)

Platelet-activating factor acetylhydrolase IB subunit beta is an enzyme that in humans is encoded by the PAFAH1B2 gene. (wikipedia.org)
This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
The regulator of calcineurin (RCAN) was first reported as a novel gene called DSCR1, encoded in a region termed the Down syndrome critical region (DSCR) of human chromosome 21. (molcells.org)
Batches of cells were infected for microarray experiments, and a quantitative polymerase chain reaction was used to verify the reproducibility of the changes in gene expression. (cdc.gov)

Moreover, EV fractions derived from HIV-infected individuals lead to greater endothelial cell death via necrotic pathways. (bvsalud.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Many signalling pathways were elucidated in cell-free systems or in intact cells. (uni-frankfurt.de)

Bax and Bak caspases) or inhibitors of the cell death processes (e.g. (immune-source.com)
The IAPs are evolutionarily conserved proteins that serve to inhibit apoptosis by binding to and inhibiting activation of caspases (24-26). (immune-source.com)

Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)
Hcy inhibits glutathione peroxidase, an enzyme that normally protects cells against oxidation . (allergyresearchgroup.blog)

In contrast DATS-treated PC-3 and LNCaP cells exhibited noticeable induction of survivin and cIAP1 proteins. (immune-source.com)
In cultured human being prostate malignancy cells DATS treatment offers Isochlorogenic acid B been shown to cause cell cycle arrest apoptosis induction and transcriptional repression of androgen receptor (13-19). (immune-source.com)
Prior function from our lab has established which the mitochondria-mediated apoptosis induction by DATS treatment is normally followed by induction of Bax and/or Bak in cultured and Isochlorogenic acid B xenografted individual prostate cancers cells (11 13 18 Nevertheless the function of IAP family members proteins in legislation of DATS-induced apoptosis is normally unclear. (immune-source.com)
After combining these two drugs, the apoptosis rate raised to 68%, which was a significant approval of synergistic apoptosis induction of Met and Lira. (ac.ir)
A doubling of Bax expression was observed in endothelial cells, indicating the induction of apoptosis in this cell type. (uni-frankfurt.de)
The increased expression of Bax seems to correlate with elevated Low Density Lipoprotein (LDL) levels in both models underlining the induction of apoptosis by elevated serum LDL. (uni-frankfurt.de)

In addition, treatment with QL (0.5 μg/ml) alone did not affect cell viability and LDH release. (biomedres.info)

Endothelial cells derived from human umbilical cord were subjected to an lipopolysaccharide (LPS) challenge. (biomedcentral.com)

LPS (also referred to as endotoxin) is one of the important virulence factors that is known to mediate inflammation and apoptosis ( 2 ). (spandidos-publications.com)
This study helped to determine the proper concentration of PYM to induce more apoptosis than necrosis, which is critical to minimize inflammation, enhance the healing of the skin, and maintain safety for the patient. (geneticsmr.com)
[31] Elevated Hcy levels produce oxidative stress , inflammation , and cell death. (allergyresearchgroup.blog)
Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)

Not only in HeLa cells, HBSS-induced lactate production and PDH phosphorylation were also observed in CL1.5, A431 and human umbilical vein endothelial cells. (tmu.edu.tw)

We investigated whether atheroprotective unidirectional pulsatile shear stress affects the expression of PDCD4 in endothelial cells. (plos.org)
En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
To reveal the effect of endothelial sensitivity to shear stress, we compared noradrenaline-induced changes in total and proximal arterial resistances during perfusion of the hind limb at a constant blood flow and at a constant pressure in vessels with intact and injured endothelium. (shengsci.com)
In the first phase of the extravasation process, selectins such as L-selectin on leukocytes and E- and P-selectin on endothelial cells lead to a loose connection that permits tethering and rolling of leukocytes on the endothelium under hydrodynamic shear [ 10 ]. (biomedcentral.com)

However, the action of PDCD4 in endothelial cells is unclear. (plos.org)

However, few studies have investigated LPS-induced apoptosis of vascular endothelial cells. (spandidos-publications.com)

It has also been demonstrated that ECs apoptosis induced atherosclerotic lesion development and plaque shedding [ 3 - 5 ]. (biomedres.info)

In conclusion, apoptosis in advanced plaques seems to be a double edged sword: apoptosis of VSMCs may lead to plaque rupture due to diminished collagen synthesis and following myocardial infarction. (uni-frankfurt.de)

Necrotic cells were more prevalent than apoptotic cells at high PYM concentrations. (geneticsmr.com)

In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)

Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)
In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)

Ginkgolide B exerts anti-apoptotic and anti-inflammatory effects on endothelial cells via PXR activation, suggesting that a PXR-mediated endothelial detoxification program may be important for protecting endothelial cells from xeno- and endobiotic-induced injuries. (greenmedinfo.com)
The apoptotic rate was elevated initially both in IR- and non-IR-cells, but that of IR-cells was maintained at an elevated level thereafter in contrast to that of non-IR-cells decreasing promptly. (telomerescience.com)
USP47 induces cisplatin resistance in head and neck squamous cell carcinoma by up-regulation of anti-apoptotic proteins [J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(4): 434-441. (shsmu.edu.cn)

In this study, we analyzed the expression and function of the novel inhibitory receptor immunoglobulin-like transcript-2 (ILT2) on NK cells from 131 CHB patients and 36 healthy controls. (bvsalud.org)

Despite tremendous progress made in the diagnosis and managements, head and neck squamous cell carcinoma (HNSC) remains a global medical dilemma with dismal clinical prognosis and high mortality. (bvsalud.org)
Other types include squamous cell carcinoma (see the image below) and adenocarcinomas. (medscape.com)
Cross-section through the bladder, uterus, and vagina with squamous cell carcinoma of the bladder infiltrating through the bladder wall into the vaginal wall. (medscape.com)

The frequency of ILT2+CD56dimNK cells was positively correlated with serum viral load in immune-tolerant patients. (bvsalud.org)
The results showed that the TFs significantly attenuated cell injury and markedly decreased serum creatinine (Cr) and blood urea nitrogen (BUN) levels in rats. (mdpi.com)
A172 (human glioblastoma) cells were maintained at 37°C in Dulbecco modified Eagle medium (Sigma, St. Louis, MO, USA) supplemented with 10% fetal calf serum. (cdc.gov)

Tripartite Motif Containing 13 (TRIM13), a member of TRIM proteins, is deleted in multiple tumor types, especially in B-cell chronic lymphocytic leukemia and multiple myeloma. (cancerindex.org)
Bcl-2 Bcl-xL and inhibitor of apoptosis family proteins) (22-26). (immune-source.com)
Elevated manifestation of IAP proteins including X-linked inhibitor of apoptosis (XIAP) and survivin has been reported in human being prostate cancers (27-29). (immune-source.com)

Effects of exosomes derived from human umbilical vein endothelial cells on apoptosis of pre-chondrogenic cells stimulated by inflammatory factors [J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(2): 147-153. (shsmu.edu.cn)

7. Eftekhari S, Montazeri H, Tarighi P. Synergistic anti-tumor effects of Liraglutide, a glucagon-like peptide-1 receptor agonist, along with Docetaxel on LNCaP prostate cancer cell line. (ac.ir)
Administered alone, FBZ inhibited proliferation faster than VES in both mouse and human prostate cancer cell lines and a synergistic effect between both was also observed. (researchgate.net)
prostate cancer cell lines and a synergistic effect between both was also o bserved. (researchgate.net)

1997. Human neutrophils employ the myeloperoxidase hydrogen peroxide-chloride system to convert hydroxyl-amino acids into glycolaldehyde, 2-hydroxypropanal, and acrolein. (cdc.gov)
Neutrophils were freshly isolated from blood of 10 male healthy volunteers, stimulated the same way as endothelial cells and analyzed for surface expression of L-selectin, CD11b and CD18. (biomedcentral.com)

Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
Anti-angiogenic effects of Metformin, an AMPK activator, on human umbilical vein endothelial cells and on granulation tissue in rat. (ac.ir)

Effect of 1,25(OHD on the proliferation of human mesangial cells and their expression of Ki67. (geneticsmr.com)

We analyzed the response of human glioma cells to West Nile virus infection by investigating host transcriptional changes. (cdc.gov)
We conducted a global transcriptional analysis of human glioblastoma cell response to infection with WNV during peak virus production to determine the crucial virus-host interactions that take place during a severe neuroinvasive attack and identify putative mechanisms involved in WNV pathogenesis. (cdc.gov)

However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)

EVs were profiled with markers reflecting lymphoid, myeloid, and endothelial origin. (bvsalud.org)

Oxidized Low-Density Lipoprotein (ox-LDL), an important atherosclerotic risk factor, may induce ECs apoptosis during the pathogenesis of AS [ 6 - 8 ]. (biomedres.info)

iii) absence of several haematopoietic and endothelial markers (ie, CD45, CD34, CD11b or CD14, CD79 or CD19 and HLA-DR in human MSCs). (bmj.com)

Anatomy38

Organisms9

Diseases8

Chemicals and Drugs136

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes42

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science3

HUVECs15

Microvascular endothelial cells2

Dysfunction6

Induces apoptosis3

Flow cytometry3

Angiogenesis7

METHODS5

Inhibition6

Epithelial6

Nitric oxide sy2

Vascular smooth muscl2

Cytotoxicity5

Genes10

Decreased cell viability3

HUVEC5

Vitro13

Anti-proliferative1

Homeostasis2

Cardiovascular4

Protein12

Differentiation3

Atherosclerosis3

Coronary artery3

Pathway8

Mechanism7

Assay7

Gene4

Pathways3

Caspases2

Inhibits2

Induction6

Viability1

Lipopolysaccharide1

Inflammation4

Phosphorylation1

Shear5

Unclear1

Studies have investigated1

Atherosclerotic lesion1

Myocardial1

Necrotic1

STAT31

Fibroblasts2

Apoptotic3

Inhibitory1

Squamous cell carc3

Serum3

Proteins3

Inflammatory1

Synergistic3

Neutrophils2

Angiogenic5

Proliferation of human1

Transcriptional2

Progenitor cells3

Myeloid1

Pathogenesis1

CD451