AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Cell CycleCell Cycle ProteinsApoptosisCell Cycle CheckpointsCaspasesApoptosis Regulatory ProteinsCaspase 3Cell Line, TumorG1 PhaseProto-Oncogene Proteins c-bcl-2Cell DivisionInhibitor of Apoptosis ProteinsTumor Suppressor Protein p53Cell ProliferationSignal TransductionMitosisCell SurvivalS PhaseCyclinsDNA FragmentationCells, CulturedTumor Cells, Culturedbcl-2-Associated X ProteinCell LineIn Situ Nick-End LabelingFlow CytometryG2 PhaseCyclin-Dependent Kinase Inhibitor p21PhosphorylationX-Linked Inhibitor of Apoptosis ProteinBlotting, WesternDNA DamageProtein-Serine-Threonine KinasesEnzyme ActivationMenstrual CycleRetinoblastoma ProteinCyclin-Dependent Kinase Inhibitor p27G2 Phase Cell Cycle CheckpointsApoptosis Inducing FactorCaspase 9Antineoplastic AgentsMitochondriaProto-Oncogene ProteinsCaspase InhibitorsCASP8 and FADD-Like Apoptosis Regulating ProteinDNA-Binding ProteinsTime FactorsRNA, MessengerTransfectionG0 PhaseDown-RegulationCaspase 8HeLa CellsMolecular Sequence DataEnzyme InhibitorsRNA, Small InterferingTumor Suppressor ProteinsNuclear ProteinsCell NucleusFas Ligand ProteinCDC2 Protein KinaseGene Expression RegulationG1 Phase Cell Cycle CheckpointsMutationCytochromes cTranscription FactorsCyclin D1bcl-X ProteinModels, BiologicalCyclin-Dependent Kinase 2Dose-Response Relationship, DrugAnnexin A5Gene Expression Regulation, NeoplasticCell DifferentiationCyclin EDNA ReplicationCysteine Proteinase InhibitorsPoly(ADP-ribose) PolymerasesReverse Transcriptase Polymerase Chain ReactionCell DeathCyclin AGenes, cdcUp-RegulationCyclin BCarrier ProteinsFibroblastsTranscription, GeneticHL-60 CellsInterphaseAmino Acid Chloromethyl KetonesDNABase SequenceAmino Acid SequenceCyclin-Dependent Kinase 4RNA InterferenceProtein BindingE2F1 Transcription FactorE2F Transcription FactorsReactive Oxygen SpeciesGene Expression
ProliferationInhibitionArrestGenesProteinsProgressionTumor cellsAutophagyInhibitorTumorsPathwaysNecrosisGrowthTreatment inhibitedViabilitySensitivityMetastasisEndothelialKinaseVitro and in vivoVivoFragmentationBreast CancerXenograftEpithelial cellsAcquired resistanceProstate cancer cellsCellularExpressionFlowCancerPartially reversedConcentration-dependent mannerNucleiChemotherapyMetaboliteRegulationNuclearPhosphorylationHaving DNA damageSquamous Cell CarcDeathTissuesSurvivalRegulatorPhaseImmuneLeukemiaDecreaseProtein synthesisTriggerTriggersReplicationConcentrationsMononuclear cellsHuman
Proliferation27
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • Here, we demonstrated that chaetocin dramatically inhibited the proliferation in ESCC cells by causing cycle arrest in the M phase and activating the caspase-dependent apoptosis signaling pathway in vitro , and we also found that chaetocin induced the accumulation cellular reactive oxygen species (ROS). (aging-us.com)
  • Moreover, the MST1/2 inhibitor XMU-MP-1 not only partially rescued the inhibitory effect chaetocin-induced proliferation, but also rescued the chaetocin-induced apoptosis in ESCC cells. (aging-us.com)
  • Moreover, Os30 can powerfully inhibit the proliferation of KC-0116 (BaF3-EGFRDel19/T790M/C797S) and KC-0122 (BaF3-EGFRL858R/T790M/C797S) cells. (bvsalud.org)
  • Additionally, GEM or GEM+CDDP at low concentrations dramatically facilitated the proliferation, migration, and invasion of tumor cells in vitro . (researchsquare.com)
  • Transcriptome analysis after ectopic transfection of synthetic miR-29b into leukemia cells indicates that miR-29b target apoptosis, cell cycle, and proliferation pathways. (ashpublications.org)
  • Functional roles of MSI2 and miR-143 on AML cell proliferation and migration were determined by CCK-8 assay, colony formation, and transwell assays in vitro and in mouse subcutaneous xenograft and orthotopic transplantation models in vivo. (biomedcentral.com)
  • Acute myeloid leukemia (AML) is a heterogenous hematological malignancy characterized by inhibited differentiation and uncontrolled proliferation of hematopoietic stem/progenitor cells (HSPC). (biomedcentral.com)
  • In addition, after the overexpression or knockdown of PAK3 in cells, the proliferation, migration and invasion abilities of these cells were assessed by Cell Counting Kit-8 (CCK-8), wound healing and Transwell assays, and the results were confirmed in vivo experiments in mice. (jcancer.org)
  • We subsequently confirmed that PAK3 was overexpressed in HCC clinical samples and HCC cell lines and that PAK3 promoted the proliferation, migration and invasion of HCC cells in vitro . (jcancer.org)
  • The IS regulates critical cell functions including molecular-regulated cellular metabolite fluxes, protein and energetic metabolism, cell proliferation and apoptosis with consequent regulation of cell life including endothelial homeostasis and blood coagulation. (biomedcentral.com)
  • However, very little data are available on IS of circulating cells of MetS patients although clinical data show that inhibited mTOR, with specific inhibitors such as Serolimus or Everolimus after kidney transplantation, significantly increase the presence of de novo thrombotic micro-angiopathy with artery lesion characterized by intimal cell proliferation, necrosis and narrowed lumen. (biomedcentral.com)
  • We found that PIk1 depletion dramatically inhibited cell proliferation, decreased viability, and resulted in cell-cycle arrest with 4 N DNA content. (uky.edu)
  • Mnt overexpression suppressed cell cycle entry and cell proliferation, suggesting that the ratio of Mnt-Max to c-Myc-Max is critical for cell cycle entry. (silverchair.com)
  • These results demonstrate that Mnt-Myc antagonism plays a fundamental role in regulating cell cycle entry and proliferation. (silverchair.com)
  • Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line. (springeropen.com)
  • Cell proliferation was measured by CCK8 and EdU assay. (besjournal.com)
  • CRC cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner, and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or paracrine. (besjournal.com)
  • Interestingly, ULK2 inhibited CRC cell proliferation in a p53-dependent manner. (besjournal.com)
  • Furthermore, exosome-derived miR-224-5p partially reversed the proliferation regulation of ULK2 on CRC cells. (besjournal.com)
  • Conclusion Our findings demonstrate that exosome-transmitted miR-224-5p promotes p53-dependent cell proliferation by targeting ULK2 in CRC, which may offer promising targets for CRC prevention and therapy. (besjournal.com)
  • the proliferation of HCT 116 cells inside a 3-Butylidenephthalide dose-dependent way whereas proliferation had not been affected in HT-29 cells. (researchensemble.com)
  • Fig. 1 The inhibitory aftereffect of mixed parthenolide (PT) and tumor necrosis element (TNF)-related apoptosis-inducing ligand (Path) treatment on cell proliferation. (researchensemble.com)
  • Among the genes that remain deregulated during remission, we identify several that are expressed by epithelial cells and are involved in epithelial cell proliferation, resistance to apoptosis and response to stress. (bmj.com)
  • It also stops proliferation of cancer cells by arresting them at different phases of the cell cycle and/or by inducing their apoptosis (programmed cell death). (beatcancer.org)
  • Both fucoxanthin and its metabolite fucoxanthinol can inhibit the proliferation of PC-3 cells, activate Caspase-3 and induce apoptosis. (zspharmac.com)
  • Acute myeloid leukemia is an aggressive malignancy characterized by uncontrolled proliferation and accumulation of immature myeloid precursor cells in the bone marrow [ 1 ]. (biomedcentral.com)
Inhibition12
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
  • Cell-cycle arrest, activation of associated apoptotic proteins, and inhibition of apoptosis were also observed in tumor cells. (researchsquare.com)
  • DNA damage occurred in PIk1-depleted cells and inhibition of ATM strongly potentiated the lethality of PIk1 depletion. (uky.edu)
  • In pediatric solid tumors and brain tumors, PLK1 inhibition leads to apoptosis in vitro and in tumor xenografts. (childrenscolorado.org)
  • The novel PLK1 inhibitor, onvansertib, is the first available for oral administration and has shown tumor growth inhibition in hematologic tumors, osteosarcoma, ovarian carcinoma, breast cancer and colon adenocarcinoma cells. (childrenscolorado.org)
  • The purpose of the current study was to determine if inhibition of protein synthesis caused by silvestrol triggers autophagy and apoptosis in cultured human cancer cells derived from solid tumors. (biomedcentral.com)
  • In addition, ebastine treatment exerted obvious effects on cell cycle arrest, metastasis inhibition, apoptosis and autophagy induction both in vitro and in vivo . (ijbs.com)
  • Co-inhibition of IGF and CHK1 caused synergistic suppression of cell viability, cell survival and tumour growth in 2D cell culture, 3D spheroid cultures and in vivo. (nature.com)
  • Investigating the mechanism of synthetic lethality, we reveal that CHK1 inhibition in IGF-1R depleted or inhibited cells further downregulated RRM2, reduced dNTP supply and profoundly delayed replication fork progression. (nature.com)
  • SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)
  • Cetuximab-bound EGF receptor inhibits activation of receptor-associated kinases, resulting in inhibition of cell growth, induction of apoptosis, and decreased production of matrix metalloproteinase and vascular endothelial growth factor. (medscape.com)
Arrest7
  • In addition, Os30 can suppress EGFR phosphorylation in a concentration-dependent manner in KC-0116 cells, arrest KC-0116 cells at G1 phase and induce the apoptosis of KC-0116 cells. (bvsalud.org)
  • A7 induced apoptosis and caused cell cycle arrest in SU-DHL-6 cells. (bvsalud.org)
  • Their non-cytotoxicity can be assigned to their ability to arrest the cell cycle at the G1 phase (Annu et al. (springeropen.com)
  • Therefore, compared to normal p53-proficient cells, p53-defective cells are more reliant on MK2 activity, which drives an alternative cell cycle checkpoint pathway that stabilizes the CKI inhibitors p27 Kip1 and Gadd45α in order to maintain G 1 /S and G 2 /M arrest after certain types of DNA damage 16 , 18 . (nature.com)
  • Fucoxanthin can inhibit the expansion of human colon cancer cells cell line WiDr in a dose-dependent fashion, arrest the cell cycle in the G0/G1 phase, and induce apoptosis. (zspharmac.com)
  • P53 exerts its effects by promoting apoptosis, cell cycle arrest, DNA repair and multiple events including senescence, autophagy and ferroptosis [ 2 ] . (encyclopedia.pub)
  • Restoration of CMTM4 significantly suppressed 786-O cell growth by inducing G2/M cell cycle arrest and p21 upregulation, and cell migration was also inhibited. (biomedcentral.com)
Genes12
  • A significant enrichment for apoptosis genes, including MCL-1 , was found among the mRNAs inversely correlated with miR-29b expression in 45 primary AML samples. (ashpublications.org)
  • Methods: Human SKNMC neuronal cells and THP1 monocytes were infected in vitro for 24-72 hrs with a laboratory strain of Chlamydia pneumoniae followed by RNA extraction, cDNA synthesis and analysis using Real-Time PCR microarrays for autophagy and inflammasome genes. (pcom.edu)
  • IL-1beta, AIM2, CCL2, and CCL7 genes were all dramatically up-regulated in monocytes during the 72 hrs of infection. (pcom.edu)
  • Discoveries have shown that melanoma frequently harbors mutations that attenuate tumor suppressor genes such as p53 and PTEN, leading to cell cycle dysregulation. (biomedcentral.com)
  • Here, we show that c-Myc induction during cell cycle entry leads to a transient decrease in Mnt-Max complexes and a transient switch in the ratio of Mnt-Max to c-Myc-Max on shared target genes. (silverchair.com)
  • Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)
  • Twenty-nine of these genes were significantly regulated in UC-in-remission subjects compared with non-IBD controls, including a large number of epithelial cell-expressed genes such as REG4, S100P, SERPINB5, SLC16A1, DEFB1, AQP3 and AQP8, which modulate epithelial cell growth, sensitivity to apoptosis and immune function. (bmj.com)
  • Prevent DNA Damage - Curcumin protects against DNA damage to genes regulating cell replication by toxins, radiation, and other environmental factors. (beatcancer.org)
  • Normalize Cell Growth - Curcumin restores normal growth controls, reverses uncontrolled reproduction of malignant cells, blocks access of cancer cells to growth factors, turns on genes that prevent cell growth and turns off genes that promote cancer growth. (beatcancer.org)
  • Indeed, emerging evidence indicates that miRNAs function as a molecular rheostats that serve in fine-tuning cell signaling pathways [ 10 , 11 ], doing so by modulating the expression of large numbers of genes and, consequently, impacting the flux through essential regulatory nodes of vast signaling networks. (biomedcentral.com)
  • The total chromosomal content of a cell involves approximately 105 genes in a specialized macromolecule of deoxyribonucleic acid (DNA). (cdc.gov)
  • The genes of cells program a process that, when triggered, results in death of the cell. (msdmanuals.com)
Proteins8
  • A key feature of prostate cancer progression is the induction and activation of survival proteins, including the Inhibitor of Apoptosis (IAP) family member survivin. (oncotarget.com)
  • We further revealed that chaetocin triggered the activation of Hippo pathway in ESCC cells, which is characterized by elevated phosphorylation levels of almost all core proteins in Hippo pathway, such as MST1 (Thr183), MST2 (Thr180), MOB1 (Thr35), LAST1 (Thr1079 and Ser909) and YAP (Ser127), ultimately leading to decreased nuclear translocation of YAP. (aging-us.com)
  • He mentioned this to David Stillman , who was at Stanford to interview for a faculty position, and who studied cell cycle regulation of proteins as a postdoc in Kim Nasmyth 's lab at the MRC Laboratory of Molecular Biology in the U.K. Stillman pointed out that ribonucleotide reductase was cell cycle regulated-rather than remaining stable, the RNA and protein levels fluctuate throughout the cell cycle. (the-scientist.com)
  • P53 produces proteins that repair damaged cells. (breastcancerconqueror.com)
  • These proteins are also catalysts for a process called apoptosis , or "programmed cell death. (breastcancerconqueror.com)
  • The central dogma of molecular biology states that the transfer of genetic information within cells transpires sequentially from DNA to RNA to proteins, whose coding sequences comprise a paltry 1.5-2% of the human genome [ 2 , 3 ]. (biomedcentral.com)
  • Cells depend on their DNA for coding information to make various classes of proteins that include enzymes, certain hormones, transport proteins, and structural proteins that support life. (cdc.gov)
  • Specialized cell structures called ribosomes are the cellular organelles that actually synthesize the proteins (RNA transcription). (cdc.gov)
Progression8
  • These findings indicate that, the continuous low-dose administration of GEM and GEM+CDDP can promote tumorigenesis and tumor progression in vivo by inhibiting apoptosis, mobilizing BMDCs, and promoting angiogenesis in certain dose ranges. (researchsquare.com)
  • This study observes a previously neglected pharmacological phenomenon and investigates its mechanism of that the continuous low-dose administration of some antineoplastic agents in certain dose ranges can promote tumorigenesis and tumor progression in vitro and in vivo , through stimulation of tumor cell functions directly as well as enhancement of tumor angiogenesis by BMDCs recruitment indirectly. (researchsquare.com)
  • Exemestane also induces apoptosis, arrests cell cycle progression at the G2/M phase and dramatically inhibits the growth of human breast cancer cells in vitro. (steroidscanada.org)
  • In addition, silvestrol blocked progression through the cell cycle at the G 2 -phase. (biomedcentral.com)
  • A report published Physica Energetics calls P53 the "master regulator of cell cycle progression. (breastcancerconqueror.com)
  • Recently, accumulating preclinical studies have illustrated the feasibility of slowing tumor progression by either overexpressing tumor suppressive miRNAs, or by neutralizing the activities of oncogenic miRNAs in cell- and animal-based models of cancer. (biomedcentral.com)
  • In addition, recent data have highlighted a role for altered p53 function in promoting the induction of immunosuppressive pathways within the tumor microenvironment (TME) [ 4 ] , thus leading to immuno-escaping of malignant cells and facilitating disease progression. (encyclopedia.pub)
  • The effects of CMTM4 on cellular apoptosis and cell cycle progression were analysed by flow cytometry, and cell cycle hallmarks were detected by western blotting and RT-PCR. (biomedcentral.com)
Tumor cells6
  • Usually, however, some tumor cells persist - and may change their behavior in response to other cells' deaths. (cancer.gov)
  • It's almost like dying tumor cells give some sort of last words to the live tumor cells so those cells can survive better," Yang says. (cancer.gov)
  • In mice, Yang's team found that the contents released from burst nuclei triggered growth-promoting signaling in nearby mammary tumor cells and accelerated the growth of metastatic tumors. (cancer.gov)
  • This innovative approach enhances the patient's immune system to target and eliminate tumor cells. (biomedcentral.com)
  • GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
  • This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)
Autophagy8
  • Results: Gene expression for autophagy and inflammasome pathways was altered dramatically following infection. (pcom.edu)
  • Conclusions: Our data suggest that Chlamydia pneumoniae-infected human SKNMC neuronal cells and THP1 monocytes exhibit specific changes in gene regulation for both autophagy and inflammasome pathways. (pcom.edu)
  • Bafilomycin A, an autophagy inhibitor, resulted in the accumulation of LC3 in cells treated with silvestrol. (biomedcentral.com)
  • Silvestrol-mediated cell death was attenuated in ATG7-null mouse embryonic fibroblasts (MEFs) lacking a functional autophagy protein. (biomedcentral.com)
  • Silvestrol potently inhibits cell growth and induces cell death in human melanoma cells through induction of early autophagy and caspase-mediated apoptosis. (biomedcentral.com)
  • Silvestrol represents a natural product scaffold that exhibits potent cytotoxic activity and could be used for the further study of autophagy and its relationship to apoptosis in cancer cells. (biomedcentral.com)
  • Mechanistically, we observed that ebastine treatment triggered proapoptotic autophagy by activating AMPK/ULK1 signaling in osteosarcoma cells. (ijbs.com)
  • Treatment with the AMPK inhibitor dorsomorphin reversed ebastine-induced apoptosis and autophagy. (ijbs.com)
Inhibitor1
  • Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)
Tumors11
  • The team suspects apoptotic cells may also help drive the growth of metastatic tumors in humans. (cancer.gov)
  • In an analysis of tumor samples from patients with breast or lung cancer, they found that outcomes tended to be worse when the genetic activity of patients' tumors indicated a high level of apoptosis-induced nuclear expulsion. (cancer.gov)
  • This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
  • Cell adhesion molecule-1 (CADM1) is a member of the immunoglobulin superfamily that functions as a tumor suppressor of lung tumors. (cancerindex.org)
  • Neuroblastoma is one of the most common solid tumors of childhood, arising from immature sympathetic nervous system cells. (biomedcentral.com)
  • NBs belong to the subgroup of small round blue cell tumors and can often pose a challenge to the pathologist because of their similarities with lymphomas, rhabdomyosarcomas, the Ewing family of tumors and desmoplastic round cell tumors. (biomedcentral.com)
  • Because most tumors are deficient in one or more aspects of the function of the p53 tumor suppressor, either as a consequence of mutations within p53, or impairment of upstream and downstream modulators of p53 activity 19 , targeting MK2 has the potential to selectively enhance tumor cell killing without increasing the genotoxic effects of chemotherapy on normal p53-wild type tissues. (nature.com)
  • Both laboratory and live animal studies demonstrate that the compound can dramatically reduce the occurrence and severity of malignant tumors. (beatcancer.org)
  • 1) Skin cancer cells fucoxanthin can prevent the enhanced task of computer mouse skin ornithine decarboxylase induced by the strong skin cancer marketer tetradecanoylphorbol-13-acetate (TPA), while chocolate hinders TPA-induced activation of human herpesvirus, thus inhibiting TPA-induced skin tumors. (zspharmac.com)
  • Although genetic and epigenetic aberrations that occur in components of the central dogma clearly elicit disease development in humans, recent findings also point to a prominent role for non-protein-coding regions of the genome in regulating cell and tissue homeostasis, as well as in contributing to the formation of human tumors. (biomedcentral.com)
  • B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)
Pathways4
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • While most molecular biologists thought signaling pathways worked by sensing signals extrinsic to the cell and relaying the information to the nucleus, Elledge was proposing an internal signaling pathway that senses cell-intrinsic events. (the-scientist.com)
  • Another hallmark of p53 mutated cancer is reprogramming of several metabolic pathways, including glycolysis, TCA (tricarboxylic acid cycle), purine and pyrimidine biosynthesis, fatty acid metabolism and iron metabolism [ 3 ] . (encyclopedia.pub)
  • A number of direct and indirect radiation interaction pathways can produce damage to the DNA of irradiated cells. (cdc.gov)
Necrosis2
  • Background/Aims Combination therapy utilizing tumor necrosis element (TNF)-related apoptosis-inducing ligand (TRAIL) together with other anticancer real estate agents is a promising technique to overcome TRAIL level of resistance in malignant cells. (researchensemble.com)
  • Fig. 2 The apoptotic effect of combined parthenolide (PT) and tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) treatment. (researchensemble.com)
Growth15
  • Cancer cells that die via apoptosis (larger dark purple structures) expel their nuclear contents (orange and yellow stringy structures) to spur metastasis and growth of living cancer cells (smaller light blue structures). (cancer.gov)
  • When cancer cells die, they leave behind signals that spur the growth of the cells they've left behind, according to a new study led by Li Yang, Ph.D. , Senior Investigator in the Laboratory of Cancer Biology and Genetics . (cancer.gov)
  • They say it may be possible to block those signals to prevent cells that die during cancer therapy from provoking the growth of any cancer cells that remain. (cancer.gov)
  • More importantly, Os30 showed potent antitumor efficacy in the KC-0116 cells xenograft nude mice tumor model with the tumor growth inhibitory rate of 77.6% at a dosage of 40 mg/kg. (bvsalud.org)
  • We found that MSI2 was significantly overexpressed in AML and exerted its role of promoting AML cell growth by targeting DLL1 and thereby activating Notch signaling pathway. (biomedcentral.com)
  • Dysregulation of various cells in the tumor microenvironment (TME) causes immunosuppressive functions and aggressive tumor growth. (biomedcentral.com)
  • The oncogenic kinase PLK1 controls cell cycle and growth and is a cancer therapeutic target. (childrenscolorado.org)
  • Cell cycle and DNA replication are top signaling hubs for medulloblastoma cell growth. (childrenscolorado.org)
  • Curcumin can help protect against cancer initiation, slow its growth when it occurs, and even sensitize cancer cells to treatment. (beatcancer.org)
  • Fucoxanthin can dramatically hinder the growth of colon cancer cells cell lines, consisting of Caco-2, HT-29 and DLD-1. (zspharmac.com)
  • 3) The effect of hematological growth fucoxanthin on severe myeloid leukemia HL-60 cell line. (zspharmac.com)
  • 5) Liver cancer fucoxanthin has a repressive result on the growth of human liver cancer cells HepG2 cells. (zspharmac.com)
  • P53 is similar to the BCRA gene in that, when it is working properly, it actually protects against the excessive growth of cancer cells. (breastcancerconqueror.com)
  • Cell Counting Kit-8 (CCK-8) and viable cell counting assays were used to delineate the growth curves of 786-O cells after CMTM4 overexpression or knockdown. (biomedcentral.com)
  • The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. (biomedcentral.com)
Treatment inhibited2
Viability3
  • In vitro cell viability, flow cytometry, fluorescence microscopy, qPCR and immunoblot was used to study the mechanism of action of silvestrol in MDA-MB-435 melanoma cells. (biomedcentral.com)
  • Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • Path alone didn't inhibit cell success (significantly less than 10%) whereas mixed treatment with PT exhibited a will dependent decrease in cell viability (Fig. 1B). (researchensemble.com)
Sensitivity2
  • Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. (wikipedia.org)
  • Numerous studies have confirmed that in addition to interfering with the tumor inflammatory environment, anti-inflammatory agents can directly increase apoptosis and sensitivity to conventional therapies and decrease invasion and metastasis, making them useful candidates for cancer therapy. (ijbs.com)
Metastasis1
  • Currently, the clinical interventions for patients with BC-bone metastasis (BC-BM) are bisphosphonates (BPs) or denosumab (an anti-receptor activator of nuclear factor kappa B ligand (RANKL) monoclonal antibody), which disrupt the vicious cycle by targeting osteoclasts [ 6 ]. (biomedcentral.com)
Endothelial3
  • Tumor and endothelial cell functions, apoptosis, cell cycle analysis, as well as bone marrow derived cells (BMDCs) mobilization, were evaluated with transwell, MTT or flow cytometry analysis in vitro , respectively. (researchsquare.com)
  • The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
  • Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
Kinase6
  • The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
  • Erikson, Raymond L. / Polo-like kinase (Plk)1 depletion induces apoptosis in cancer cells . (uky.edu)
  • Polo-like kinase-1 (PLK1) is highly expressed in rapidly dividing normal cells and is overexpressed in many types of cancer, including Group 3 medulloblastoma, and is associated with poor prognosis. (childrenscolorado.org)
  • The protein expressions of p53 and unc-51 like kinase 2 (ULK2) in CRC cells were detected by western blot. (besjournal.com)
  • With a better understanding of the underlying pathophysiology of chronic lymphocytic leukemia and the introduction of novel targeted agents, such as Bruton kinase Inhibitors, the prognosis of high-risk patients has changed dramatically. (pharmacytimes.com)
  • In response to DNA damage, a synthetic lethal relationship exists between the cell cycle checkpoint kinase MK2 and the tumor suppressor p53. (nature.com)
Vitro and in vivo1
  • The compound has been found to show promising in vitro and in vivo activities against certain B-cell malignancies [ 12 ], and has been under preclinical toxicogical development in the National Cancer Institute Experimental Therapeutics (NExT) program. (biomedcentral.com)
Vivo2
  • These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)
  • Overexpressing circIKBKB enhanced the capability of BC cells to induce formation of bone pre-metastatic niche dramatically by promoting osteoclastogenesis in vivo and in vitro. (biomedcentral.com)
Fragmentation4
  • Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′- hydroxyl termini in the double-strand DNA breaks generated during apoptosis. (wikipedia.org)
  • TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. (wikipedia.org)
  • On the other hand upon treatment with both agencies HT-29 cells exhibited apoptotic features including cell shrinkage nuclear condensation and nuclear fragmentation. (researchensemble.com)
  • Generates DNA fragmentation in colon cancer cells, advertises cell apoptosis, and prevents the expression of apoptosis-related protein Bcl-2. (zspharmac.com)
Breast Cancer1
  • Aiming to exploit this effect in therapy we performed a compound screen in five breast cancer cell lines with IGF neutralising antibody xentuzumab. (nature.com)
Xenograft2
  • Restoration of miR-29b in AML cell lines and primary samples induces apoptosis and dramatically reduces tumorigenicity in a xenograft leukemia model. (ashpublications.org)
  • The cell line derived xenograft mouse model was established to confirm the results of the in vitro experiments. (biomedcentral.com)
Epithelial cells2
  • 2016 ). This study was conducted to analyze the anticancer effects of green-synthesized AgNPs using Juniperus polycarpos on A549 (adenocarcinomic human alveolar basal epithelial cells) cell lines, as well as comparing it with Cisplatin commercial drug. (springeropen.com)
  • In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)
Acquired resistance1
  • The EGFRC797S mutation is a dominant mechanism of acquired resistance after the treatment of non-small cell lung cancer (NSCLC) with osimertinib in clinic. (bvsalud.org)
Prostate cancer cells3
  • First, given evidence that IGFs regulate the response to IR, we also found evidence that IGF-1R depletion induced endogenous DNA lesions marked by γH2AX foci in prostate cancer cells [ 10 ]. (nature.com)
  • 4. As medicine, used for the treatment of skin cancer cells, colon cancer cells, prostate cancer cells, liver cancer cells and various other cancers cells. (zspharmac.com)
  • 4) Prostate cancer cells fucoxanthin can dramatically lower the survival rate of prostate cancer cells and generate cell apoptosis. (zspharmac.com)
Cellular2
  • The TME consists of various cellular components, including immune cells, stromal cells, and extracellular matrix, along with soluble factors and signaling molecules. (biomedcentral.com)
  • Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. (springeropen.com)
Expression10
  • Accumulating data have confirmed that high expression of Msi2 mRNA is associated with poor prognosis in AML, as patients with as low as 1% of total bone marrow cells expressing elevated MSI2 levels still have a short survival [ 14 , 15 ]. (biomedcentral.com)
  • Subsequently, the expression of PAK3 was further confirmed in HCC cells. (jcancer.org)
  • An investigation of PLK1 expression in DepMap found PLK1 is a critical mediator in 55 central nervous system cell lines, including eight medulloblastoma cell lines. (childrenscolorado.org)
  • By 24 h, a decrease in cyclin B and cyclin D expression was observed in silvestrol-treated cells relative to control. (biomedcentral.com)
  • Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR, respectively. (besjournal.com)
  • Wild-type p53 itself acts by regulating and promoting the tricarboxylic acid (TCA) cycle, for instance by enhancing cytochrome c oxidase assembly (SCO2) expression to maintain cytochrome c oxidase complex and increasing Parkin levels in cells, which increases pyruvate dehydrogenase E1α1 (PDHA1), a critical component of the pyruvate dehydrogenase complex [ 11 ] . (encyclopedia.pub)
  • Primary clear cell renal cell carcinoma (ccRCC) and the paired adjacent non-tumour tissues were then collected to examine the expression of CMTM4 by western blotting, immunohistochemistry, and quantitative RT-PCR. (biomedcentral.com)
  • The ccRCC cell lines A498 and 786-O and the normal renal tubular epithelial cell line HK-2 were also tested for CMTM4 expression by western blotting. (biomedcentral.com)
  • G-CSF upregulated the expression of AQP9 and enhanced the intracellular concentrations of ATO in AML cells. (biomedcentral.com)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
Flow5
  • The fluorochrome-based TUNEL assay applicable for flow cytometry, combining the detection of DNA strand breaks with respect to the cell cycle-phase position, was originally developed by Gorczyca et al. (wikipedia.org)
  • Flow cytometry was used to detect cell cycle and apoptosis. (besjournal.com)
  • 24 hours after incubation with one or 3-Butylidenephthalide both agents cells were analyzed by PI staining and flow cytometric analysis. (researchensemble.com)
  • Flow cytometry was performed to evaluate the cell apoptosis levels. (biomedcentral.com)
  • In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
Cancer22
  • In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • Yang says her team's findings highlight the fact that individual cancer cells are part of a community of cells whose members influence one another's behavior - even in their final moments. (cancer.gov)
  • Within cancer cell communities, she says, death is commonplace. (cancer.gov)
  • And when a patient undergoes cancer treatment, the number of dying cancer cells in the body rises still further. (cancer.gov)
  • Various stressors that cancer cells naturally encounter in the body can trigger apoptosis. (cancer.gov)
  • The researchers' first step was to watch cancer cells under a microscope as they underwent apoptosis. (cancer.gov)
  • Collaborating with Steven D. Cappell, Ph.D. , Stadtman Investigator in the Laboratory of Cancer Biology and Genetics, who applies state-of-the-art live-cell imaging approaches in his cell cycle research, they saw the nuclei of dying cells swell dramatically and then burst, spewing their DNA and other contents into their surroundings. (cancer.gov)
  • We used the recently developed vector-based small interfering RNA technique to specifically deplete PIk1 in cancer cells. (uky.edu)
  • This review examines how LSD1 modulates different cell behavior in TME and emphasizes the potential use of LSD1 inhibitors in combination with ICB therapy for future cancer research studies. (biomedcentral.com)
  • However, cancer cells exploit these checkpoints to evade immune surveillance and suppress antitumor immune responses. (biomedcentral.com)
  • Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. (springeropen.com)
  • AgNPs are utilized for treating different types of cancerous cells due to their extraordinary biomedical features (anti-cancer, anti-bacterial, anti-viral, and anti-angiogenic properties) as well as surface-specific characteristics (Burdușel et al. (springeropen.com)
  • Stimulate Apoptosis - Curcumin encourages programmed cancer cell death. (beatcancer.org)
  • Enhance Immune Surveillance - Curcumin prevents cancer cells from evading normal immune system detection and improves the body's ability to detect and destroy malignant cells. (beatcancer.org)
  • Inhibit Cancer Stem Cells - Curcumin makes cancer stem cells (normally resistant to chemotherapy and radiation) more sensitive to treatment and thereby less likely to allow cancer to regrow. (beatcancer.org)
  • Reduce Production of Adhesion Molecules - Curcumin keeps malignant tissue from sticking to vessel walls and reduces cancer cells' ability to form distant metastases. (beatcancer.org)
  • 2) Colon cancer cells fucoxanthin has a repressive result on the formation of duodenal cancer cells caused by N-ethyl-N'- nitro-N-nitrosoguanidine. (zspharmac.com)
  • When it does not function properly, however, cancer risk can raise dramatically. (breastcancerconqueror.com)
  • If it cannot be repaired, P53 will signal for the cell to undergo apoptosis, or cancer cell death. (breastcancerconqueror.com)
  • Despite this rather bleak outlook, the collective efforts of science and medicine have nevertheless dramatically reduced the annualized cancer death rate by 20% over the last two decades, thereby sparing the lives of more than 1.3 million Americans [ 1 ]. (biomedcentral.com)
  • In this study, the anti-cancer effects of pentadecanoic acid were evaluated in human breast carcinoma MCF-7/stem-like cells (SC), a cell line with greater mobility, invasiveness, and cancer stem cell properties compared to the parental MCF-7 cells. (mdpi.com)
Partially reversed1
  • PIk1-depletion-induced apoptosis was partially reversed by cotransfection of nondegradable mouse PIk1 constructs. (uky.edu)
Concentration-dependent manner1
  • A1, A5 and A7 showed significant inhibitory effects against SU-DHL-6 cells and effectively inhibited Akt phosphorylation in a good concentration-dependent manner. (bvsalud.org)
Nuclei4
  • PIk1 depletion induced apoptosis, as indicated by the appearance of subgenomic DNA in fluorescence-activated cell-sorter (FACS) profiles, the activation of caspase 3, and the formation of fragmented nuclei. (uky.edu)
  • There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
  • Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
  • Skeletal muscle is fairly unique in that the mature cells are syncytial and can contain hundreds of nuclei. (frontiersin.org)
Chemotherapy3
  • Although localized disease can be treated by surgery alone, the standard therapy for patients with metastatic disease usually comprises intensive induction chemotherapy, local surgery and myeloablative chemotherapy followed by autologous stem cell transplant, external radiotherapy and retinoic acid treatment. (biomedcentral.com)
  • However, the conventional chemotherapy with antineoplastic agents at the maximum tolerated dose (MTD) is typically administrated in short cycles, obligatorily separated by long treatment breaks (e.g., 3 weeks), and unfortunately accompanied by serious toxic side effects. (researchsquare.com)
  • Cells may be damaged by harmful substances, such as radiation, sunlight, and chemotherapy drugs. (msdmanuals.com)
Metabolite1
  • Fucoxanthin and its metabolite fucoxanthinol can prevent the survival of T cells contaminated by human T-celllymphotropic infection kind 1 (HTLV-1) and grown-up T-cell leukemia cells. (zspharmac.com)
Regulation1
  • and (iii) the dramatic reductions to lymphocyte numbers and function 1-2 h after exercise reflects a transient and time-dependent redistribution of immune cells to peripheral tissues, resulting in a heightened state of immune surveillance and immune regulation, as opposed to immune suppression. (frontiersin.org)
Nuclear2
  • In silvestrol-treated cells, DAPI staining of nuclear chromatin displayed nucleosomal fragments. (biomedcentral.com)
  • Up coming cells had been stained with Hoechst 33258 and visualized by confocal microscopy to look for the existence apoptotic nuclear morphology. (researchensemble.com)
Phosphorylation3
  • The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)
  • It can obstruct the cells in the G0/G1 phase and prevent the phosphorylation of Rb protein Ser780 site. (zspharmac.com)
  • In particular, it is now well-established that the core leukemic cell population differs from leukemic stem cell (LSC) in the bioenergetics program, with the latter mainly relying on oxidative phosphorylation (OXPHOS) rather than on anaerobic glycolysis. (encyclopedia.pub)
Having DNA damage1
Squamous Cell Carc2
  • Li L , Jiang H , Li Y , Xiang X , Chu Y , Tang J , Liu K , Huo D , Zhang X , . Chaetocin exhibits anticancer effects in esophageal squamous cell carcinoma via activation of Hippo pathway. (aging-us.com)
  • HPV infections have received particular attention in recent years, as high-risk strains have been linked to some cases of oral squamous cell carcinoma. (medscape.com)
Death9
Tissues3
  • These checkpoints act as molecular brakes on immune cells, preventing excessive activation and potential damage to healthy tissues. (biomedcentral.com)
  • Fucoxanthin has excellent antioxidant results, even far better than vitamin E and vitamin C. Fucoxanthin has a protective effect on human fiber cell damage caused by UV-B. The antioxidant result of fucoxanthin is mostly with managing the task of Na+- K+- ATPase, as well as the task of catalase and glutathione in tissues and molecules brought on by retinol shortage. (zspharmac.com)
  • Western blotting then confirmed that CMTM4 was dramatically reduced in 86.9 % (53/61) of ccRCC tissues compared with the paired adjacent non-tumour tissues, as well as in the 786-O and A498 ccRCC cell lines. (biomedcentral.com)
Survival1
  • TP53 mutated/deleted acute myeloid leukemia (AML) stands out as one of the poorest prognosis forms of acute leukemia with a median overall survival not reaching one year in most cases, even in selected cases when allogenic stem-cell transplantation is performed. (encyclopedia.pub)
Regulator2
  • The c-Myc oncoprotein is strongly induced during the G0 to S-phase transition and is an important regulator of cell cycle entry. (silverchair.com)
  • More importantly, we found that IPMK interacted with AMPK and functioned as a positive regulator of AMPK protein in osteosarcoma cells. (ijbs.com)
Phase1
  • The method has subsequently been improved dramatically and if performed correctly should only identify cells in the last phase of apoptosis. (wikipedia.org)
Immune1
  • Indeed, the behavior of almost all immune cell populations in the bloodstream is altered in some way during and after exercise ( 7 , 8 ). (frontiersin.org)
Leukemia2
  • The use of base editing to generate universal, off-the-shelf CAR T cells is a promising approach for relapsed leukemia, with potential implications for the future of gene therapy. (pharmacytimes.com)
  • In types of human being leukemia, dBET1 induced an instant reduced amount of BRD4 and c-MYC, and triggered apoptosis. (p38-mapk-inhibitors.com)
Decrease1
  • May involve decrease in reticuloendothelial cell function or increase in platelet production. (medscape.com)
Protein synthesis1
  • Accordingly, mTOR influences the energy metabolism, protein synthesis, cell cycles and reparative processes including anti-apoptotic effects which are fundamental for cell life span. (biomedcentral.com)
Trigger1
Triggers1
  • Other triggers include an excess number of cells and possibly damage to a cell. (msdmanuals.com)
Replication2
  • Elledge's idea that eukaryotic cells sense the progress of DNA replication and transform that information into a DNA-damage response was new. (the-scientist.com)
  • Those results led him to study how cells monitor roadblocks to replication and DNA damage, such as nicks and double-stranded breaks, and how the cell handles that information. (the-scientist.com)
Concentrations2
  • A) HT-29 and HCT 116 cells had been treated with Path every day and night in the concentrations indicated … To look for the synergistic aftereffect of PT on TRAIL-induced cell loss of life HT-29 cells had been incubated in the lack or existence of PT (10 μM) and Path (5 25 or 40 ng/mL). (researchensemble.com)
  • The bloom spanned three months, during which time cell concentrations exceeded human guideline thresholds for recreational risk, and concentrations of the hepatotoxic cyanotoxin nodularin exceeded 200 µg L(-1). (omicsdi.org)
Mononuclear cells2
  • Total mononuclear cells were isolated from peripheral blood using density gradient centrifugation. (biomedcentral.com)
  • However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Human3
  • 2018 ). It was also confirmed that AgNPs synthesized by the Juniperus chinensis leaf showed the potent anticancer effect on the human adenocarcinoma gastric (AGS) cell lines (Al-Dhafri and Ching 2019 ). (springeropen.com)
  • Within the scope of this trial, we investigated the effects of the natural pine bark extract pycnogenol on human fibrosarcoma cells. (spandidos-publications.com)
  • The human body has nearly 1013 cells. (cdc.gov)