• Learn about cell viability assays. (abcam.com)
  • Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combina tion or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). (scirp.org)
  • Their in vitro effects on hypoxic cells concerning cell viability, apoptosis, migration, and angiogenesis were detected through gain- and loss-of-function assays. (hindawi.com)
  • The application of established cell viability assays such as the commonly used trypan blue staining method to coral cells is not straightforward due to different culture parameters and different cellular features specific to mammalian cells compared to marine invertebrates. (nature.com)
  • This work presents the approach used to customize dye pairs for membrane integrity-based cell viability assays considering the species- and genotype-specific autofluorescence of scleractinian corals, namely: endogenous fluorescence characterization followed by the selection of dyes that do not overlap with endogenous signals. (nature.com)
  • Fluorescent indicator dyes (fluorophores) are most commonly used in biochemical and cell-based assays in vertebrate cell culture since fluorescence is more sensitive 3 and fluorescence is utilized in many instruments (microscopy, spectroscopy, flow cytometry). (nature.com)
  • Table 1 includes common fluorophores used to assess membrane integrity and distinguish between live and dead cells when conducting viability assays. (nature.com)
  • In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. (cdc.gov)
  • Relative cell viability was assessed using MTT assays. (wjgnet.com)
  • The Tali Image-based Cytometer is a 3-channel benchtop cytometer that uses image analysis to perform basic cellular assays and cell counting on cells in suspension. (epfl.ch)
  • With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)
  • Cytotoxic effects of fipronil and fipronil sulfone were examined using the adenylate kinase and the trypan blue exclusion assays in HepG2 cells and human hepatocytes. (cdc.gov)
  • In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
  • MLE-12 cells were induced by LPS to construct an ARDS model in vitro. (karger.com)
  • CONCLUSIONS: Taken together, these data demonstrate that despite its sustained expression, Beclin-1 is dispensable for autophagy induction in ovarian tumor cells in vitro, yet may be retained to promote cell viability by a mechanism independent of autophagy or apoptosis regulation. (bepress.com)
  • In Vitro Toxicity Studies: Cell viability (dye exclusion): trypan blue assay etc. (cdc.gov)
  • In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc. (cdc.gov)
  • In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc. (cdc.gov)
  • H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
  • Therefore, the current study used integrated in vitro and in silico approaches to figure out Amy and Sor's possible synergistic activity in targeting AMPK/mTOR and BCL-2 for anti-angiogenesis and apoptosis cell death in HepG2 cells. (biomedcentral.com)
  • In Vitro Cell Dev Biol. (aaem.pl)
  • To evaluate the neuroprotective and neurite outgrowth effects of maltol, a natural aroma compound, on retinal ganglion cells (RGCs) under oxidative stress in vitro. (molvis.org)
  • Furthermore, the authors expanded the study by conducting in vitro experiments on human skeletal muscle cells to understand the biochemical mechanisms regulating the effect of simvastatin on irisin expression. (medscape.com)
  • These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. (scirp.org)
  • The antitumor effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in gliomas has thus far only been thoroughly established in tumor cell lines. (aacrjournals.org)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450. (bdbiosciences.com)
  • Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450 in pancreatic carcinoma cell lines treated with a low dose of cetuximab targeted gold nanoparticles. (bdbiosciences.com)
  • As the RF field power increases, the temperature increases, and a shift from apoptosis (lower right quadrant) to frank necrosis (upper left quadrant) is seen. (bdbiosciences.com)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)
  • This inhibition of AMPK ultimately leads to inhibition of mTOR and thus induces apoptosis in the HepG2 cells. (biomedcentral.com)
  • Carbamazepine induces mitotic arrest in mammalian Vero cells. (aaem.pl)
  • Recent research demonstrated that the potential to induce apoptosis through endoplasmic reticular (ER) stresses can be a target for cancer therapy. (hindawi.com)
  • Conclusion: Prion protein 106-126 peptide can induce apoptosis in differentiated PC12 cells and present cellular toxicity definitely. (researchgate.net)
  • In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • Stander M, Dichgans J, Weller M. Anticonvulsant drugs fail to modulate chemotherapy-induced cytotoxicity and growth inhibition of human malignant glioma cells. (aaem.pl)
  • Based on ROS production and cell-particle interactions, overall cellular cytotoxicity was measured. (cdc.gov)
  • In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
  • Due to the pleiotropic effect of bortezomibenhanced TRAIL DISC formation upon TRAIL triggering, down-regulation of cFLIP L and activation of the intrinsic apoptosis pathway seem to cooperatively contribute to the antitumor effect of bortezomib/TRAIL cotreatment. (aacrjournals.org)
  • Loss of mitochondrial membrane potential, upregulation of proapoptotic proteins, activation of MCL-1, PARP-1 and Caspase 9 suggest that MESF treatment leads to activation of mitochondrial pathway of apoptosis. (nature.com)
  • The death receptor pathway plays an important role in cell apoptosis ( 12 ). (spandidos-publications.com)
  • The mitochondrial pathway is also referred to as the endogenous apoptosis pathway ( 15 ). (spandidos-publications.com)
  • Studies also show that Sirt1 regulates apoptosis through other pathways, such as the anti-inflammatory pathway of NF-κB acetylation and the regulation of AMPK in autophagy ( 6 , 7 ). (frontiersin.org)
  • GSEA showed that the insulin-signaling pathway was influenced in Chl1 -silenced cells. (lu.se)
  • Involvement of C/EBPβ-related signaling pathway in methamphetamine-induced neuronal autophagy and apoptosis. (cancerindex.org)
  • Caspases-9, -8 and -3 have been implicated in apoptosis-caspase-9 in the mitochondrial pathway, caspase-8 in the Fas/CD95 pathway, and caspase-3 more downstream, activated by multiple pathways. (bdbiosciences.com)
  • We have reported that many insults can trigger cells to activate a cellular death pathway (Nature, 361:739-742, 1993), that several viruses encode proteins to block attempted cell suicide (Proc. (hopkinsmedicine.org)
  • LCN2 was increased by SOX9 and SIRT1 deacetylated SOX9 to promote its nuclear translocation, which further inhibited the viability of human retinal pigment epithelial cells and promoted cell apoptosis and angiogenesis as well as CNV-induced AMD formation. (hindawi.com)
  • G: Levels of epithelial-mesenchymal transition-related proteins (E-cadherin and N-cadherin) in AGS and HGC-27 cells determined using western blotting. (wjgnet.com)
  • A human Pancreatic duct epithelial cells line (HPDE), HEK-293T and three human pancreatic cancer cell lines (CanPan-1, Hs-766T, CFPAC-1) were obtained from Chinese typical culture preservation center. (jcancer.org)
  • The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)
  • Within minutes after a small corneal epithelial injury, cells at the edge of the abrasion begin to migrate centripetally to cover the defect rapidly at a rate of 60-80 µm/h. (medscape.com)
  • Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)
  • After 24-30 hours, mitosis begins to restore epithelial cell population. (medscape.com)
  • A deficiency of limbal stem cells, from either disease (eg, aniridia) or trauma (eg, chemical burn), can preclude adequate epithelial wound healing. (medscape.com)
  • Metalloproteinases are secreted as proenzymes by neutrophils infiltrating the wound, injured epithelial cells, and keratocytes. (medscape.com)
  • Other particles may be engulfed by epithelial cells, primarily in the vicinity of the bronchial-alveolar duct junctions, and retained for much longer periods, with gradual removal to lymph nodes. (cdc.gov)
  • We aim to explore whether astragaloside IV is effective for ARDS treatment in a lipopolysaccharides (LPS)-induced cell model and whether autophagy is involved in the therapeutic function of astragaloside IV. (karger.com)
  • Astragaloside IV might suppress autophagy initiation directly or indirectly through suppressing the oxidative stress and inflammatory response, which further enhances the cell viability and tight junction and reduces apoptosis in LPS-stimulated pulmonary endothelial ARDS cell model, thus exerting its therapeutic function in ARDS. (karger.com)
  • In the present study, our aim was to determine the time-dependent, ER-mediated apoptotic and autophagy induction of purvalanol in HCT 116 colon cancer cells. (spandidos-publications.com)
  • however, purvalanol triggered autophagy, which functions as a cell survival mechanism at early time-points. (spandidos-publications.com)
  • Nonetheless, autophagy is known to be readily inducible in ovarian cancer cells. (bepress.com)
  • Knockdown of BECN1 and other autophagy-related gene expression was achieved using siRNA in established human ovarian cancer cell lines (CaOV3, OVCAR8, SKOV3, and HeyA8) and a novel early-passage, ascites-derived cell line (iOvCa147-E2). (bepress.com)
  • Our previous study has shown that CCAAT-enhancer binding protein β (C/EBPβ) is an important regulator in METH-induced neuronal autophagy and apoptosis. (cancerindex.org)
  • We hypothesized that C/EBPβ is involved in METH-induced DDIT4-mediated neuronal autophagy and Trib3-mediated neuronal apoptosis. (cancerindex.org)
  • We tested our hypothesis by examining the effects of silencing C/EBPβ, DDIT4, Trib3 or α-syn with small interfering ribonucleic acid (siRNA) on METH-induced autophagy and apoptosis in the human neuroblastoma SH-SY5Y cells. (cancerindex.org)
  • We isolated primary tumor cells from 13 astrocytoma and oligoastrocytoma patients of all four WHO grades of malignancy and compared the levels of TRAIL-induced apoptosis induction, long-term tumor cell survival, caspase, and caspase target cleavage. (aacrjournals.org)
  • In this area, our recent research has included studying cutaneous shave biopsies for diagnosing primary colonic adenocarcinoma as well as growth inhibition and apoptosis in human brain tumor cell lines using selenium. (hopkinsmedicine.org)
  • The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
  • These results suggest that the induction of apoptosis by the propolis derivative, GS-002, is partially mediated through ER stress and ATF-3-dependent pathways, and GS-002 has the potential for development as an antitumor drug. (hindawi.com)
  • In the past decade, induction of apoptosis has become the major strategy to combat cancer. (hindawi.com)
  • Therefore, a search for innovative strategies other than induction of apoptosis is urgently needed. (hindawi.com)
  • RSV can improve mitochondrial function, reduce oxidative stress in podocytes, and inhibit apoptosis induction through the Sirt1/PGC-1a axis ( 12 ). (frontiersin.org)
  • Objective: To investigate the induction of tanshinone [1 A (Tan fl A) on the differentiation of human placenta-derived mesenchymal stem cells (hPDMSCs) into cardiomyocytes, and to provide an experimental basis for Tan IT A as a cardiomyocytc differentiation inducer. (researchgate.net)
  • A significant time- and dose-dependent induction of adenylate kinase activity in HepG2 cells was noted from 0.1 to 12.5 µM fipronil followed by decreasing activities at 25 and 50 µM. (cdc.gov)
  • The possibility that cytotoxic effects were due to apoptosis was indicated by significant time- and dose-dependent induction of caspase-3/7 activity in both HepG2 cells and human hepatocytes. (cdc.gov)
  • MemBrite™ Fix dyes covalently label cell surface proteins, and are available in with a wide selection of bright and photostable colors. (biotium.com)
  • They belong to a class of growth factors, secreted proteins that can signal particular cells to survive, differentiate, or grow. (wikipedia.org)
  • They are small proteins that secrete into the nervous system to help keep nerve cells alive. (wikipedia.org)
  • The main issue with trypan blue is its capacity to bind to proteins in the cell suspension, not just that released by dead cells. (nature.com)
  • Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
  • During the first stage called G1, cells monitor their environment and, when the requisite signals are received, the cells synthesize RNA and proteins to induce growth. (bdbiosciences.com)
  • Of particular interest to our group are the mechanisms by which Bcl-2 family proteins and other factors regulate programmed cell death, particularly in the nervous system, in cancer and in virus infections. (hopkinsmedicine.org)
  • We have shown that anti-apoptotic Bcl-2 family proteins can be converted into killer molecules (Science 278:1966-8, 1997), that Bcl-2 family proteins interact with regulators of caspases and regulators of cell cycle check point activation (Molecular Cell 6:31-40, 2000). (hopkinsmedicine.org)
  • Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)
  • Granulosa-theca cell tumors, more commonly known as granulosa cell tumors (GCTs), belong to the sex cord-stromal category and include tumors composed of granulosa cells, theca cells, and fibroblasts in varying degrees and combinations. (medscape.com)
  • However, the maltol treatment restored the cells in a dose-dependent manner. (molvis.org)
  • Overexpression of LINC00673 promotes invasion and metastasis which associated with a poor prognosis in tongue squamous cell carcinoma [ 6 ] . (jcancer.org)
  • Neurotrophic factors are secreted by target tissue and act by preventing the associated neuron from initiating programmed cell death - allowing the neurons to survive. (wikipedia.org)
  • Angiotensin II Activates Extracellular Signal Regulated Kinases via Protein Kinase C and Epidermal Growth Factor Receptor in Breast Cancer Cells," Journal of Cellular Physiology, Vol. 196, No. 2, 2003, pp. 370-377. (scirp.org)
  • Enhancement of HER-2 degradation by carbamazepine in breast cancer SKBR-3 cell line]. (aaem.pl)
  • However, the effect of Tan IIA on apoptosis and its underlying mechanism remains elusive in oral squamous cell carcinoma (OSCC). (jcancer.org)
  • It is important to systematically combine multiple endpoints, such as membrane integrity and cell death mechanism, to gain insight into the potential pathways involved in cellular toxicity. (nature.com)
  • Τheir impact on the apoptotic cell death mechanism requires further elucidation. (spandidos-publications.com)
  • Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
  • In those cases, multiple aspects of apoptosis might need to be analyzed to confirm the mechanism of cell death. (bdbiosciences.com)
  • LINC00673 Overexpression is associated with good survival in pancreatic cancer patients, Effects of LINC00673 on pancreatic cancer cell apoptosis, viability, migration. (jcancer.org)
  • Silencing of Chl1 impairs β-cell function by disrupting the activity of key signaling pathways of importance for insulin biosynthesis and secretion. (lu.se)
  • 2 Apoptosis is triggered by caspase-mediated signal transduction pathways. (bdbiosciences.com)
  • Therefore, many genetically programmed cell suicide pathways have evolved to promote long-term survival of species from yeast to humans. (hopkinsmedicine.org)
  • The use of trypan blue, a colorimetric, cell impermeant stain, to quantify cell death is the most reported method in relation to coral cell cultures despite its limitations. (nature.com)
  • Such cells control their degree of innervation (the number of axon connections) by the secretion of various specific neurotrophic factors that are essential for neuron survival. (wikipedia.org)
  • In this study, we performed a serial of studies to investigate how Chl1 affects insulin secretion in INS-1 cells. (lu.se)
  • Silencing of Chl1 in INS-1 cells was done to assess its impact on the insulin secretion, content, cell viability, and apoptosis. (lu.se)
  • Silencing of Chl1 in INS-1 cells markedly reduced insulin content and secretion. (lu.se)
  • Methods: The human multiple myeloma SKO-007 cells in logarithm growth phase were selected and randomly divided into control group, LDM group, BZM group, and BZM combined with LDM group. (researchgate.net)
  • Methods: After differentiated by nerve growth factor (NGF), the PC12 cells were infected by prion protein 106-126 peptide. (researchgate.net)
  • Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
  • However, resistance to apoptosis is considered to be a characteristic of several types of cancers. (hindawi.com)
  • However, some cell types do not display characteristic features of apoptosis. (bdbiosciences.com)
  • With each replication event the amount of dye in the cell is decreased, leading to a characteristic pattern. (bdbiosciences.com)
  • Moreover, we determined that during purvalanol-mediated ER stress, autophagic machinery was also activated prior to apoptotic cell death finalization. (spandidos-publications.com)
  • Fipronil mediated activation of caspase-3/7 in concurrence with compromised ATP production and viability are attributed to apoptotic cell death. (cdc.gov)
  • Additionally, evidence has revealed that enhancing Sirt1 activity can reduce ROS production, reduce inflammation of neurons and glial cells, so as to reduce neuronal cell death ( 8 ). (frontiersin.org)
  • Interestingly, cell death regulators also regulate many other cellular processes prior to a death stimulus, including neuronal activity, mitochondrial dynamics and energetics. (hopkinsmedicine.org)
  • Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
  • Improve your apoptosis detection skills with our free apoptosis application ebook. (abcam.com)
  • To support this spectrum of requirements, BD Biosciences offers a full range of apoptosis detection tools and technologies for measuring indicators at different stages across the apoptotic process. (bdbiosciences.com)
  • Apoptosis refers to the self-regulated and orderly death of cells controlled by genes in order to maintain the stability of internal environment ( 8 ). (spandidos-publications.com)
  • Mesenchymal stem cells (MSCs) are the most widely used stem cells of the human b. (icbcongress.com)
  • In the present study, we investigated the therapeutic potential of TRAIL in primary human glioma cells. (aacrjournals.org)
  • We established a stable culture model for isolated primary human glioma cells. (aacrjournals.org)
  • To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)
  • Experiments were conducted on human synoviocytes derived from an RA patient and rabbit synoviocytes cell line HIG-82. (aaem.pl)
  • Billions of cells die per day in the human body. (hopkinsmedicine.org)
  • Insufficient cell death underlies human cancer and autoimmune disease, while excessive cell death underlies human neurological disorders and aging. (hopkinsmedicine.org)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • The results indicate both that HepG2 cells and primary human hepatocytes are sensitive to the cytotoxic effects of fipronil. (cdc.gov)
  • whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells grown on mouse feeders, stained for pluripotent markers NANOG (red) and OCT4 (green). (lu.se)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • This leads to the loss of cellular structure and function, and ultimately results in cell death. (bdbiosciences.com)
  • Pack LR, Daigh LH, Meyer T. Putting the brakes on the cell cycle: mechanisms of cellular growth arrest. (bdbiosciences.com)
  • This delay is required for preparatory cellular changes prior to rapid cell movement. (medscape.com)
  • Cell viability was estimated by cell counting kit-8 and cell apoptosis by flow cytometry. (karger.com)
  • The rate of apoptosis was determined using flow cytometry. (spandidos-publications.com)
  • The results of CCK‑8 and flow cytometric analyses showed that pretreatment of HUVECs with NAC or p38MAPK inhibitor (SB203580) attenuated LPS‑induced decrease in cell viability and increase in cell apoptosis. (spandidos-publications.com)
  • Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. (wjgnet.com)
  • Flow cytometry was used to detect cell apoptosis. (medscimonit.com)
  • The Flow Cytometry Laboratory at Auburn University provides operator-managed and assisted support for flow cytometry analyses and high-speed cell sorting. (auburn.edu)
  • The Flow Cytometry Laboratory offers flow cytometry analysis on a Beckman Coulter Analytical Flow Cytometer - a CytoFLEX LX flow cytometer - which is used for the qualitative and quantitative measurement of biological and physical properties of cells and other particles. (auburn.edu)
  • The AU Flow Cytometry & High-Speed Cell Sorting Core Laboratory has recently installed a new state-of-the-art compact benchtop cell sorter that is housed within a BSL2-competant laminar flow biosafety cabinet. (auburn.edu)
  • BD Biosciences tools use multiple methodologies including flow cytometry , bioimaging and microscopy (for live and fixed cell analysis) as well as ELISA , IHC , western blot and spectrofluorometry. (bdbiosciences.com)
  • During apoptosis, PS translocates to the outer leaflet of the plasma membrane and can be detected by flow cytometry and cell imaging through binding to fluorochrome-labeled annexin V when calcium is present. (bdbiosciences.com)
  • a - c The expression of Ki67, Bcl-2, Bax, and cleaved Caspase-3 in MH7A cells was detected by western blot. (biomedcentral.com)
  • Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)
  • Conclusion: BZM can greatly enhance the efficacy of LDM against multiple myeloma by increasing the levels of cleaved caspase-3 and PARP, and remarkably increase the apoptosis induced by LDM through further activation of ERS. (researchgate.net)
  • A caspase activation assay was used to assess apoptosis, and an MTT assay was employed to determine cell viability. (cdc.gov)
  • In contrast to cell lines, isolated primary tumor cells from all investigated glioma patients were highly TRAIL resistant. (aacrjournals.org)
  • Regardless of the tumor heterogeneity, cotreatment with the proteasome inhibitor bortezomib efficiently sensitized all primary glioma samples for TRAIL-induced apoptosis and tremendously reduced their clonogenic survival. (aacrjournals.org)
  • As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
  • CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. (biotium.com)
  • We also offer classic membrane and membrane potential dyes, including CellBrite™ and CellBrite™ NIR dyes , which are lipophilic carbocyanine dyes that stain the plasma membrane of cells and are non-toxic for long-term tracking. (biotium.com)
  • It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells. (biotium.com)
  • DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. (biotium.com)
  • 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C 18 carbon) hydrocarbon chains into the lipid bilayer. (biotium.com)
  • It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. (cancerindex.org)
  • Changes in the plasma membrane are one of the characteristics of the apoptotic process detected in living cells. (bdbiosciences.com)
  • Apoptosis can be detected by the presence of phosphatidylserine (PS), which is normally located on the cytoplasmic face of the plasma membrane. (bdbiosciences.com)
  • Since intracellular annexin V is also exposed if the plasma membrane is compromised, a membrane-impermeant dye such as 7-AAD is commonly used to distinguish between apoptotic and dead cells to exclude the dead cells. (bdbiosciences.com)
  • Vinculin, a plasma protein, links fibers to talin, which is a cell membrane protein. (medscape.com)
  • Molecular cytogenetic analysis of the olive flounder embryonic cell line FGBC8 and its applicability to biotechnology. (nih.gov)
  • Subsequently, the effect of an additional small molecular inhibitor, BMS-777607 (which targets MST1R (RON), MET, Tyro3, and Axl) also resulted in a decreased proliferative capacity of MPM cells. (frontiersin.org)
  • Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
  • Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)
  • A substantial synergistic interaction between Amy and Sor was observed (CI 50 = 0.56), which was connected to cell cycle arrest at the S and G2/M stages and increased apoptosis and potential necroptosis. (biomedcentral.com)
  • Cells were also treated with PKC activator PEP005 or specific siRNA against protein kinase Cδ (PKCδ). (medscimonit.com)
  • SquamouS cell carcinoma for the viability of the tumour cel s once skin lesions are also mutated in the ultraviolet they have become malignant (Rol ison (UV) radiation-induced skin lesions of A large number of HPV types have et al. (who.int)
  • Table 1 Fluorophores tested on coral cells and conclusions. (nature.com)
  • Detect the early stages of apoptosis with a simple staining procedure in only 10 minutes. (abcam.com)
  • In the final stages of the process, dying cells become fragmented into apoptotic bodies and consequently are eliminated by phagocytic cells without significant inflammatory damage to surrounding cells. (bdbiosciences.com)
  • Although the vast majority of neurons in the mammalian brain are formed prenatally, parts of the adult brain (for example, the hippocampus) retain the ability to grow new neurons from neural stem cells, a process known as neurogenesis. (wikipedia.org)
  • then, tryptan blue staining was conducted to evaluate the cell viability. (icbcongress.com)
  • MTT assay was used to evaluate the cell viabilities. (medscimonit.com)
  • PUMA or Foxo3a knockdown compromised the inhibitory effect of Tan IIA on OSCC cells. (jcancer.org)
  • Beclin-1 knockdown instead decreased cell viability without inducing apoptosis. (bepress.com)
  • It is known that application of mesenchymal stem cells is an effective treatment in many diseases. (icbcongress.com)
  • Mesenchymal stem cells from bone marrow were cultured and preconditioned with serum deprivation and h2o2 for 6, 12, 24 and 48 hours in 6 groups (i: control, ii: 5μm h2o2, iii: 10μm h2o2, iv: 5%fbs, v: 5μm h2o2+5%fbs and vi: 10μm h2o2+5%fbs). (icbcongress.com)
  • Among stem cells, mesenchymal stem cells (MSCs) have been used to treat the related pathologies in animal models of AD, and other neurodegenerative disease. (j-alz.com)
  • The fibroblast-like synovial cell in rheumatoid arthritis: a key player in inflammation and joint destruction. (aaem.pl)
  • Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis. (aaem.pl)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • Baicalin treatment reduced phosphorylation levels of PKCδ and p53, as well as the expression levels of active caspase3 and bax in HAECs exposed to H/R. The treatment of PKC activator PEP005 impaired the protective effects of baicalin in increasing cell viabilities and reducing apoptosis in HAECs exposed to H/R. (medscimonit.com)
  • Regarding the cell cycle analysis, we did not observed any changes in cell cycle phases. (scirp.org)
  • Also available are post-acquisition analysis software packages that provide listmode playback of all experiments in real-time with full analytical adjustment, cell cycle analysis, histogram subtraction and/or overlays of multiple histograms, and color-backgating of discrete cell populations. (auburn.edu)
  • LPS (also referred to as endotoxin) is one of the important virulence factors that is known to mediate inflammation and apoptosis ( 2 ). (spandidos-publications.com)
  • Numerous cytokines and growth factors that are up-regulated in corneal cells further contribute to tissue inflammation. (medscape.com)
  • according to our data, the cell viabilityingroupsv and vi, which were both preconditions at all times were significantly increased compared to control group. (icbcongress.com)
  • Results showed that nanowire-exposed cells had significantly increased levels of ROS over a 7 hour time-course, while nanosphere-treated cells did not. (cdc.gov)
  • CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)