Apoptosis and cell cycle regulation apoptotic cells cell cycle. Medical search. Frequent questions

Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
si-Prok1 arrests cell cycle, induces apoptotic changes, and inhibits the proliferation of ovarian granulosa cells through a mechanism related to the regulation of Pi3k/Akt signaling pathway. (ajol.info)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
Given the strong link between the regulation of some of these genes and the growth and dissemination of gastrointestinal cancers, we believe their aberrant expression in UC may provide a mechanism for epithelial hyper-proliferation and, in the context of malignant transformation, for tumour growth. (bmj.com)
Among the genes that remain deregulated during remission, we identify several that are expressed by epithelial cells and are involved in epithelial cell proliferation, resistance to apoptosis and response to stress. (bmj.com)
Galectins are β-galactoside-binding lectins that are involved in various biologic processes, such as apoptosis, cell proliferation, and cell-cycle regulation. (elsevierpure.com)
The PI3K/Akt/mTOR signal pathway is involved in multiple cellular functions including proliferation, differentiation, tumorigenesis, and apoptosis. (biomedcentral.com)
PI3K activates a number of signaling molecules, among which the Akt/mTOR pathway is of particular interest because of its role in inhibiting apoptosis and promoting cell proliferation [ 3 ]. (biomedcentral.com)
The balance between cell proliferation and death is vital for normal growth, differentiation, and tissue homeostasis. (oncotarget.com)
Apoptotic death is preceded by proliferation blockage, suggesting that these transcripts are involved in cell cycle regulation. (uandes.cl)
Our results suggest that ASncmtRNA targeting blocks tumor cell proliferation through reduction of essential cell cycle proteins, mediated by mitochondrial and nuclear miRNAs. (uandes.cl)
Furthermore, silencing PrP c expression with si-PRNP amplified the fucoidan-induced changes in cell proliferation, apoptosis, and migration. (iiarjournals.org)
Apoptosis is also implicated in homoeostasis by fine-tuning the balance between cell death and proliferation like in gonad size control in rodents [ 11 ]. (biomedcentral.com)
Accumulating evidence in many metazoans suggest that apoptotic cells can emit caspase-dependent signals to their neighbours, modulating their fate (survival/death, differentiation or proliferation) or behaviour (migration) [ 12 , 13 ]. (biomedcentral.com)
Apoptotic-induced proliferation was reported in several organisms, such as in the cnidarian Hydra , where apoptosis promotes proliferation of adjacent cells through emission of Wnt3 signalling in a caspase-dependant manner during head regeneration [ 14 ]. (biomedcentral.com)
The current study demonstrated that PD markedly inhibited PAC cell proliferation and triggered their apoptosis. (biomedcentral.com)
Various assays were used to investigate the stimulatory effects of the purified rFg14-3-3e protein on specific functions of goat PBMCs, including cytokine secretion, proliferation, migration, nitric oxide (NO) production, phagocytosis, and apoptotic capabilities. (biomedcentral.com)
Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)
Many laboratories have focused on the functions of the clock genes and proteins that are involved in cellular activities, including cell survival, cell cycle regulation, proliferation, apoptosis, DNA damage repair, and tumor promotion or suppression [ 5 , 6 ]. (explorationpub.com)
Instead, the mutant cells influence the behavior of neighboring wild-type (non-mutant) cells and promote their proliferation and increased apoptotic resistance, causing non-autonomous overgrowth. (umassmed.edu)
PGM1 knockdown stimulated both cell proliferation and colony formation, and inhibited cell cycle arrest and apoptosis, while overexpression of PGM1 produced the opposite effects in CRC cells both in vivo and in vitro. (biomedcentral.com)
PGM1 inhibits hepatocellular carcinoma cell (HCC) proliferation and growth by utilizing sufficient extracellular glucose to convert glycogen, while deletion of the PGM1 gene inhibits glycogen synthesis and leads to glycolysis of additional glucose, thus promoting tumor cell proliferation and growth [ 15 ]. (biomedcentral.com)
MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
MicroRNAs control the levels of numerous cell cycle regulators that controls cell proliferation. (ukessays.com)
As a consequence, any modulations of the target transcript expression, miRNAs can affect various signalling pathways and cellular process such as apoptosis, proliferation or differentiation. (ukessays.com)
HMG-CoA reductase is widely used as a significant target to screen cardiovascular therapeutics molecules because of its involvement in cholesterol synthesis and cell proliferation. (waocp.com)
Steroidal drugs like anastrozole, exemestane, letrozole have been tested as potential inhibitors of cancer mediated aromatase enzyme and these could be inhibited to HMG-CoA reductase so that the link with cholesterol synthesis and cell proliferation can be utilized to control cancers in. (waocp.com)
To test the binding affinity and binding patterns using computational methods of these drugs and established them as a HMG-CoA reductase inhibitor due to the involvement of this enzyme with cholesterol synthesis and cell proliferation can be utilized to control the cancers. (waocp.com)

N-terminal acetylation is one of the most common co-translational covalent modifications of proteins in eukaryotes, and it is crucial for the regulation and function of different proteins. (wikipedia.org)
Cell mitosis is an attractive target to effectively treat a variety of cancer forms, where microtubule-associated proteins (MAPs) assist cells to maintain the stability of cell dynamics. (medicalxpress.com)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
Western blot analysis revealed that VALD-3 upregulated pro-apoptotic proteins (Bad and Bax), downregulated anti-apoptotic proteins (Bcl-2, Bcl-xl, survivin and XIAP) and increased the expression of cleaved caspase-3, cleaved caspase-8, Cyto-c and cleaved PARP. (researchsquare.com)
Based on observations that in addition to driving growth and transformation, activated K-Ras was also powerfully pro-apoptotic, we used an in silico approach to identify potential novel Ras binding proteins that might mediate the growth inhibitory properties of Ras. (louisville.edu)
These pathways link RASSF proteins into the control of motility, genetic stability, apoptosis, the cell cycle, regulation of protein stability, senescence and DNA repair. (louisville.edu)
GO enrichment analysis of the interacting proteins identified terms related to apoptosis, protein binding, locomotion, hippo signalling and leukocyte and lymphocyte differentiation, supporting the experimental findings. (biomedcentral.com)
To achieve this ordering, many cell cycle-regulatory proteins are expressed exclusively when their functions are needed. (umassmed.edu)
In addition, they are investigating how cell cycle-regulation of chromatin proteins helps to coordinate the condensation of chromosomes with their segregation during mitosis. (umassmed.edu)
LPLI-induced cell cycle progression can be regulated by the activation or elevated expressions of cell cycle-specific proteins. (biomedcentral.com)
T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
It has been previously shown that rbf1 pro-apoptotic activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. (sdbonline.org)
Both proteins can localize at the mitochondrion , but the way they control apoptosis still remains unclear. (sdbonline.org)
These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis and shed light on a link between Rbf1 and mitochondrial dynamics, in vivo. (sdbonline.org)
Taken at face value, senile cataract is intimately associated with the increased risk of oxidative stress, which levies irrevocable changes in the lenticular proteins that impel the lens epithelial cells to the apoptotic phase. (molvis.org)
Our results indicate that several dozens of proteins for each cell type, are PE-lipoxidated in HT-22, MLE, and H9c2 cells and M2 macrophages after they were induced to undergo ferroptosis. (cdc.gov)
Pretreatment of cells with the strong nucleophile, 2-mercaptoethanol, prevented the formation of PE-lipoxidated proteins and blocked ferroptotic death. (cdc.gov)

The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)
This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
Background: The putative functions of the cellular prion protein (PrP c ) are believed to be associated with cell signaling, differentiation, survival, and cancer progression. (iiarjournals.org)
We propose these results as a confirmation that apoptotic cells can control surrounding cells fate and as a reference database to explore novel apoptotic functions in animals, including those related to migration and differentiation. (biomedcentral.com)
Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)

To investigate the regulatory influence of Prok1 on apoptotic and proliferative changes in PCOS, and the implication of Pi3k/Akt/nf-κ B signaling pathway in the process. (ajol.info)
VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)

Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
It has long been noted that caspase-2-deficient cells proliferate faster [2], and elegant studies have shown that caspase-2 induces cell cycle arrest following cytokinesis failure [5]. (nature.com)
In contrast, while caspase-2-deficiency led to an increased proportion of S-phase cells and a progressive decrease in the proportion of G1-phase cells following treatment with arresting concentrations of the DNA damaging agent camptothecin and increased DNA damage following S-phase arrest, overexpression of Bcl-X L did not change the cell cycle profiles or the amount of DNA damage. (nature.com)
While other groups have demonstrated non-apoptotic roles for caspase-2 in DNA repair, cell cycle arrest [2], this is the first demonstration that these roles are regulated by a distinct pathway to apoptosis. (nature.com)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
In this study, the effect of Gal-9 on Gl-phase cell-cycle arrest, one of the hallmark pathologic changes in early diabetic nephropathy, was investigated. (elsevierpure.com)
In many cell lines, exposure to rapamycin results in a relatively small decrease in overall protein synthesis (~15-20%), but dose result specifically in G1 cell cycle arrest. (biomedcentral.com)
This work adds to the elucidation of the molecular mechanisms behind cell cycle arrest preceding tumor cell apoptosis induced by ASncmtRNA knockdown. (uandes.cl)
Our results demonstrate that Pulsatillae chinensis and particularly its bioactive ingredient β-peltatin suppress PAC by triggering cell cycle arrest at G2/M phase and apoptosis. (biomedcentral.com)
1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
While exploring the molecular changes associated with strong ST efficacy in breast cancer cells, we observed that ST induced cell cycle arrest as well as cell death. (biomedcentral.com)
ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)

A re-evaluation of parameters of cell kinetics in view of our increasing knowledge of the molecular pathways of cell cycle control may provide more prognostic information for the management of patients with malignant lymphomas. (bmj.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
When caspase activity was impaired, genes known to be involved in metamorphosis were downregulated along with other implicated in cell migration and survival molecular pathways. (biomedcentral.com)
Activation of apoptosis signaling pathways is tightly linked with the generation of reactive oxygen species (ROS). (cdc.gov)
Melatonin can disrupt estrogen-mediated cellular pathways, ensuing in a lattice drop of estrogenic stimulus to the breast cancer cells. (explorationpub.com)
The Kim lab investigates how various metabolic pathways, such as amino acid biosynthesis pathways, in a tumor cell relative to normal tissues. (umassmed.edu)
Evidence presented in this review implicated that Rhizoma coptidis exerted beneficial effects on various diseases by regulation of NF-κB/MAPK/PI3K-Akt/AMPK/ERS and oxidative stress pathways, which support the clinical application of Rhizoma coptidis and offer references for future researches. (springer.com)
NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
AMPK pathways are energy regulation pathways. (springer.com)

These non-apoptotic mechanisms may underlie the role of caspase-2 as a tumor suppressor. (nature.com)
In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
Cell uptake and apoptotic assay were used for the NPs internalization and apoptosis mechanisms. (bvsalud.org)
Defects in apoptosis regulatory mechanisms contribute to carcinogenesis induced by Cr(VI). (cdc.gov)
A major focus of this work is to define mechanisms that control the genesis and function of cancer stem cells with an emphasis on the role of integrin and VEGF signaling. (umassmed.edu)
The Shaw lab investigates mechanisms by which carcinoma cells acquire the ability to metastasize, with a focus on breast cancer. (umassmed.edu)

By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)
However, proliferative indices alone do not suffice to characterise cell growth. (bmj.com)
Here, we show that ASncmtRNA knockdown induces cell death preceded by proliferative blockage in three different human breast cancer cell lines. (uandes.cl)
Normally, cells which have lost a classical tumor suppressor gene become highly proliferative and resistant to apoptosis, thus permitting autonomous tumor growth. (umassmed.edu)
Method: Anti-proliferative and apoptotic effect of various doses of essential oil from Ferula asafoetida was investigated on colon cancer cell lines i.e. (ac.ir)
Result: The potent anti-proliferative activity has been observed in both the cell lines. (ac.ir)
rbf1 , the Drosophila homolog of Rb , also displays a pro- apoptotic activity in proliferative cells. (sdbonline.org)
These results suggest prominent in vitro anti-proliferative and pro-apoptotic effects of ST in MDA-MB-231 cells. (biomedcentral.com)

Our results further revealed that the synergistic interaction was linked to increased reactive oxygen species generation and apoptosis. (biomedcentral.com)
Interestingly, rbf1 -induced apoptosis leads to a debcl - and drp1 -dependent Reactive Oxygen Species production, which in turn activates the Jun Kinase pathway to trigger cell death. (sdbonline.org)
Oxidative stress is a plethora of reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide, hydroxyl radical, and inducible nitric oxide species that exert a constellation of aberrant changes in the oxidation-reduction status of the cell. (molvis.org)

However, existing mitosis-targeting chemotherapeutic agents do not discriminate between healthy and malignant cell lines, resulting in severe side-effects . (medicalxpress.com)
Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
Supports sensitization of tumors to radiation via apoptosis (cell death) cascade of tumor mass and improves oxygen delivery to malignant tissues (cancer cells) (12). (alphascreed.com)

In agreement with this observation, we found that Cr(VI) induces apoptosis mainly through the mitochondrial death pathway via caspase-9 activation, which is negatively regulated by the antiapoptotic protein Bcl-2. (cdc.gov)

The exact mechanism of fen-retinide-induced cell death is not fully understood, although it is reported to induce apoptosis indepen-dently of retinoic acid receptor expression [6-9], lacking the carboxyl group required for binding to these receptors [10]. (lsd1receptor.com)
Hexavalent chromium [Cr(VI)] compounds are redox cycling environmental carcinogens that induce apoptosis as the primary mode of cell death. (cdc.gov)

The Drosophila retinoblastoma protein, Rbf1, induces a debcl and drp1 -dependent mitochondrial apoptosis. (sdbonline.org)

Despite its pro-apoptotic role, caspase-2-deficient tumors have comparable basal apoptosis levels to wild-type tumors [3, 4]. (nature.com)
Collectively, our findings identify a novel mechanism of cyclin E-mediated Mcl-1 regulation that provides a rationale for clinical use of Bcl-2 family and Cdk inhibitors for Mcl-1-dependent tumors. (oncotarget.com)
We identified the RASSF1 gene and showed that its protein served as a K-Ras apoptotic effector that was frequently down-regulated in primary tumors. (louisville.edu)
The lab aims to elucidate advantages these metabolic alterations can play in the growth and progression of tumors, and how these changes can be exploited to selective target cancer cells, such as selectively inducing the accumulation of toxic metabolites to poison cancer cells within the tumor. (umassmed.edu)

Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
Furthermore, in tumour necrosis factor alpha (TNFa)-stimulated Ewing's sarcoma (ES) cells, NF-jB is reported to prevent activation of an apoptotic cascade through inhibition of c-Jun N-terminal kinase (JNK) [23] and upregulation of anti-oxidant enzymes [24]. (lsd1receptor.com)
CDK inhibition by dinaciclib resulted in Bim release from Mcl-1 in ABT-737-resistant cells. (oncotarget.com)
Since both natural supplements and direct regulation of PrP c expression contribute to inhibition of cancer progression and growth, we hypothesized that knockdown of PrP c could lead to an enhanced synergic effect on the inhibition of cancer growth by fucoidan. (iiarjournals.org)
Conclusion: Combination of fucoidan with silencing of PrP c has a synergic effect on the inhibition of HT29 colon cancer cell growth. (iiarjournals.org)
Melatonin has oncostatic properties that are mediated by induction of apoptosis, arresting the cell cycle, regulation of metabolic activity, and the inhibition of hypoxia-inducible factor 1α (HIF-1α) activity [ 9 ]. (explorationpub.com)
In MDA-MB-231 cells, ST caused a significant dose-dependent cell growth inhibition by 31- 63% (p ≤ 0.0001) in 48 h and 40-50% (p ≤ 0.0001) in 72 h. (biomedcentral.com)

NF-jB knockdown did not induce death under normal growth conditions, but did reduce TNFa-dependent cell survival. (lsd1receptor.com)
Enhanced apoptosis was correlated with loss of pro-survival factors (XIAP, bcl-2, bcl-xL), expression of pro-apoptotic markers (caspases 3/7, PARP cleavage) and enhanced cell cycle regulators p21 and p27. (biomedcentral.com)
Here, we show that cyclin E/Cdk2 phosphorylates and stabilizes the pro-survival Bcl-2 family protein Mcl-1, a key cell death resistance determinant to the small molecule Bcl-2 family inhibitors ABT-199 and ABT-737, mimetics of the Bcl-2 homology domain 3 (BH3). (oncotarget.com)
Previously, we have shown that the tail regression step during metamorphosis, characterized by a predictable polarized apoptotic wave, ensures elimination of most tail cells and controls primordial germ cells survival and migration. (biomedcentral.com)
Finally, apoptotic cells modulate death or survival balance, as was observed in the Drosophila imaginal disc, by producing the Tie ligand Pvf1, that induces death resistance of neighbouring cells [ 17 ]. (biomedcentral.com)
Only two Bcl-2 family genes have been found in Drosophila melanogaster including the pro-cell survival, human Bok-related orthologue, Buffy. (sdbonline.org)
Taken all together this demonstrates a role for Buffy, a Bcl-2 pro-cell survival gene, in neuroprotection. (sdbonline.org)

Cyclin E/Cdk2 kinase activity is frequently deregulated in human cancers, resulting in impaired apoptosis. (oncotarget.com)
Cyclin E levels were elevated and there was increased association of cyclin E/Cdk2 with Mcl-1 in ABT-737-resistant compared to parental cells. (oncotarget.com)
Cyclin E depletion in various human tumor cell-lines and cyclin E -/- mouse embryo fibroblasts showed decreased levels of Mcl-1 protein, with no change in Mcl-1 mRNA levels. (oncotarget.com)
Cyclin E knock-down restored ABT-737 sensitivity to acquired and inherently resistant Mcl-1-dependent tumor cells. (oncotarget.com)
This effect is mediated by downregulation of the key cell cycle progression factors cyclin B1, cyclin D1, CDK1, CDK4, and survivin, the latter also constituting an essential inhibitor of apoptosis, underlying additionally the onset of apoptosis. (uandes.cl)
The treatment also induces an increase in the microRNA hsa-miR-4485-3p, whose sequence maps to ASncmtRNA-2 and transfection of MDA-MB-231 cells with a mimic of this miRNA induces cyclin B1 and D1 downregulation. (uandes.cl)
Results: Fucoidan treatment significantly inhibited growth and reduced cyclin and cyclin-dependent kinase (CDK) expression in HT29 colon cancer cells. (iiarjournals.org)

Cell-cycle distribution analyses revealed that Gal-9 maintained further progression of cell cycle from the G1 phase. (elsevierpure.com)
Vital for regulation of gene expression, cell cycle progression, and DNA repair, and cell death (9). (alphascreed.com)

Moreover, ethanolic extracts of I'm-Yunity-Too exhibited more potent activity compared to its aqueous extracts with regard to suppression of the growth and induction of apoptosis, as assayed by the more pronounced downregulation of phosphorylation of Rb and increased cleavage of poly(ADP‑ribose) polymerase (PARP) from its native 112-kDa form to the inactive 89-kDa product. (spandidos-publications.com)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
The findings demonstrate the enhanced cell viability and the induction of antioxidant enzyme activity through the modulation of Nrf2 and Keap1 gene expression. (bvsalud.org)
β-peltatin initially arrested PAC cells at G2/M phase, followed by apoptosis induction. (biomedcentral.com)
This study reveals a novel mechanism linking .O(-)(2) with Bcl-2 stability and provides a new dimension to ROS-mediated Bcl-2 downregulation and apoptosis induction. (cdc.gov)
Further, cell death effect of ST was associated with induction of apoptosis. (biomedcentral.com)

These results suggest that chemotherapeutic agent 5-Fu may down-regulate telomerase activity at both transcriptional level and PI3K/Akt/mTOR pathway-dependent post-transcriptional level to facilitate hepatocellular carcinoma cell apoptosis. (biomedcentral.com)

Treating SMMC-7721 cells with Rapamycin, a specific mTOR inhibitor, significantly reduce hTERT protein level but did not affect hTERT transcription. (biomedcentral.com)
We performed differential transcriptomic analysis between control metamorphosing larvae and larvae treated with the pan-caspase inhibitor Z-VAD-fmk in order to explore the transcriptional control of apoptotic cells on neighbouring cells that survive and migrate. (biomedcentral.com)

In particular, we found that Cul3 controls the abundance of Plastin 3 (Pls3), a novel player of neural cell migration, whose amount is inversely proportional to neural cell migration speed. (nature.com)
Finally, we found that CRISPR-mediated activation of Cul3 transcription fully rescues neural cell migration defects. (nature.com)
Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Moreover, MCT4 overexpression enhances cell migration and invasiveness via reorganization of the actin cytoskeleton. (hindawi.com)

Protein acetylation (and deacetylation) are acetylation reactions that occur within living cells as drug metabolism, by enzymes in the liver and other organs (e. g., the brain). (wikipedia.org)
With the synchronized output of the central circadian clock in relation to the pineal gland and other peripheral oscillators, melatonin acts as a key role player in the regulation of intermediary metabolism and cancer prevention [ 7 ]. (explorationpub.com)

CONCLUSION: TSBRs destroyed cancer cells by starvation and chemotherapy in both in-vitro and in-vivo settings which demonstrates its anti-cancer potential. (bvsalud.org)
Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
Purpose: The current investigation was conducted with the aim to elucidate and determine the anti-cancer efficacy in vitro on colon carcinoma human cell lines and to determine its probable mode of action. (ac.ir)

Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)
The goal was accomplished via overexpression and knock-down of Thap1 in the HEK293T cell line and in mouse striatal primary cultures and western blotting of embryonic Thap1 -null tissue. (biomedcentral.com)

Cell cycle ratio and apoptosis in the two groups were determined using flow cytometry, while Pi3k/Akt signal route-linked protein levels were assayed by immunoblot method. (ajol.info)
The cell cycle distribution was analyzed through PI staining and flow cytometry analysis, while apoptotic cells were measured by double staining with Annexin V-FITC and PI. (biomedcentral.com)

PURPOSE: This study aims at chemotherapy and starvation therapy of HCC via starvation and apoptosis. (bvsalud.org)
Drinking grape juice 30 minutes before meals for a week following each cycle of chemotherapy doesn't seem to reduce nausea or vomiting caused by chemotherapy. (medlineplus.gov)

The glycogen synthase kinase-3β/nuclear factor-kappa B pathway is involved in cinobufagin-induced apoptosis in cultured osteosarcoma cells. (uni-muenster.de)

Gal-9 reduced glomerular expression of TGF-β1 and the number of p27 Kip1- and p21 Cip1 -positive cells in glomeruli. (elsevierpure.com)
Cisplatin (CDDP) sensitizes human osteosarcoma cell to Fas/CD95-mediated apoptosis by down-regulating FLIP-L expression. (uni-muenster.de)
Materials and Methods: PrP c expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. (iiarjournals.org)
By contrast, CD23 expression in mice is limited to B cells, follicular dendritic cells and enterocytes [14, 19, 20]. (treatmentforprostatecancer.info)
Evaluating the capacity of caspases to affect gene expression and influence fate and/or behaviour of cells adjacent to apoptosis is the first fundamental step to better characterise this new function in animals. (biomedcentral.com)
We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
The same was also confirmed in the expression studies which shows significant upregulation of the antiapoptotic characteristics and down regulation of pro-carcinogenic factors. (ac.ir)
Expression of aromatase is found in ovaries, extragonadal tissues, brain, fat, liver, endothelium bones and mesenchymal cells of adipose tissue in the breast. (waocp.com)
C-PC treatment possibly prevented cataractogenesis triggered by sodium selenite, by regulating the lens crystallin, redox genes, and apoptotic cascade mRNA expression and thus maintains lens transparency. (molvis.org)
Immunopathologic examination during infection showed cleavage of caspase 3 in brain cells adjacent to CD8+ cells and widespread p53 expression, hallmarks of apoptosis. (cdc.gov)

LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
Twenty-nine of these genes were significantly regulated in UC-in-remission subjects compared with non-IBD controls, including a large number of epithelial cell-expressed genes such as REG4, S100P, SERPINB5, SLC16A1, DEFB1, AQP3 and AQP8, which modulate epithelial cell growth, sensitivity to apoptosis and immune function. (bmj.com)
However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. (biomedcentral.com)
Because of its involvement in F. gigantica recognition by innate immune cells, rFg14-3-3e protein may have applications for development of diagnostics and therapeutic interventions. (biomedcentral.com)
Subsequently, normal cell-cell interactions between the tumor and surrounding tissues and immune cells begin to breakdown, leading to invasion and eventual metastasis to distant sites. (umassmed.edu)
Fuels CD8 and CD4 T- cell-mediated immunity (immune cells) in cancer tissue (48,49). (alldayphysique.com)
Enhanced apoptosis in the leukocytes of peripheral blood of PEM patients may be a marker of increased infection and immune disturbances. (who.int)
We focus on the characterization of the central nervous system (CNS) immunologic response to VSBV-1 by IHC analyses of immune cells in the brain of all patients, as well as by examination of cerebrospinal fluid (CSF) reactions over time during the disease. (cdc.gov)

Dose-dependent response of CKAP5 knockdown across various cancer cell lines. (medicalxpress.com)
Cells that did not show any effect of CKAP5 knockdown are highlighted with red boxes. (medicalxpress.com)
ASncmtRNA knockdown with an antisense oligonucleotide induces massive apoptosis in tumor cell lines, without affecting healthy cells. (uandes.cl)

The NF-jB family of transcription factors are involved in the regulation of several physiological processes such as inflammation, cell cycle regulation and apoptosis. (lsd1receptor.com)

The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas. (louisville.edu)
While caspase-dependent signals emitted by apoptotic cells start to be characterised, almost no target genes are identified in receptor cells. (biomedcentral.com)
If these genes are mutant, it is not the mutant cells which are overgrowing. (umassmed.edu)
Total RNA was isolated on P16, and the relative abundance of mRNA of the crystallin structural genes, redox components, and apoptotic cascade were ascertained with real-time PCR with reference to the internal control β-actin . (molvis.org)
Real-time PCR analysis showed the crystallin genes ( αA- , βB1- , γD- ) and redox cycle components ( Cat , SOD-1 , Gpx ) were downregulated, the apoptotic components were upregulated, and antiapoptotic Bcl-2 was downregulated in Group II. (molvis.org)

We detected apoptosis of hepatocarcinoma cells by TUNEL assay. (biomedcentral.com)
Cell viability was determined using Cell Counting Kit-8 assay. (biomedcentral.com)
Cell growth assay, cell cycle analysis, FACS, JC-1 staining, annexin V staining and immunoblotting were used to study the efficacy of ST on cancer cells. (biomedcentral.com)

If apoptosis is not an essential mechanism for its tumor suppression function, then a role in regulating the cell cycle may be. (nature.com)
BAY 11-7082-induced cell death through an NF-jB-independent mechanism and enhanced cell death when combined with fenretinide. (lsd1receptor.com)
however, its role in Cr(VI)-induced apoptosis and the underlying mechanism are largely unknown. (cdc.gov)

Some studies also suggest that mTOR may be a cellular context-dependent, pleiotropic regulator of apoptosis, although conclusive demonstration of mTOR inactivation in such circumstances is lacking [ 19 ]. (biomedcentral.com)
The environmental light-dark cycle is the potent regulator of melatonin synthesis. (explorationpub.com)
RRM1 , a ubiquitously expressed subunit of ribonucleoside-diphosphate reductase, and CCAR1 (cell cycle and apoptosis regulator 1) have been identified as direct targets for regulation by Thap1 in endothelial or Jurkat cells. (biomedcentral.com)

The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)
This gene thus plays a role in both cell-cycle and epigenetic regulation. (cancerindex.org)
In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)
Conclusion: The essential oil shows potent anti-cancer activity and shows the role in gene regulation to attenuate the colon cancer. (ac.ir)
This study demonstrates that debcl and the pro-fission gene drp1 are necessary downstream of buffy to trigger a mitochondrial fragmentation during rbf1 -induced apoptosis. (sdbonline.org)

In this work, Chatterjee and the team screened the effect of CKAP5 silencing in solid cancer cell lines and in normal non-cancer epithelial cell lines as a negative control. (medicalxpress.com)

In cisplatin-resistant cell lines, BT potentiated cisplatin-induced cytotoxicity at most drug ratios via enhanced ROS generation and modulation of key regulators of apoptosis. (biomedcentral.com)

There was a significantly higher apoptosis in si-Prok1 group. (ajol.info)
Caspase-2-deficient cells show a significantly higher percentage of S-phase-associated chromosomal aberrations. (nature.com)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
Animal study confirmed that β-peltatin significantly suppressed the growth of subcutaneously-implanted BxPC-3 cell xenografts. (biomedcentral.com)
CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)

Inflammatory infiltrates in areas positive for VSBV-1 RNA and antigen consisted of CD4+ and CD8+ T cells, with perivascular B-cell accumulation. (cdc.gov)
Its unique signature that distinguishes it from other forms of cell death is the formation and accumulation of lipid hydroperoxides, particularly oxidized forms of polyunsaturated phosphatidylethanolamines (PEs), which drives cell death. (cdc.gov)

Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
May support proper cell growth and DNA synthesis (65). (alphascreed.com)
ST (30-90 μM) treatments for 48 h and 72 h did not show any significant effect on cell growth and death in A431 cells. (biomedcentral.com)
Whereas similar ST treatments moderately inhibited the growth of A549 cells by up to 13% (p ≤ 0.05) in 48 h and 14% (p ≤ 0.05-0.0001) in 72 h. (biomedcentral.com)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

The versatile regulation of intracellular redox components is the primary factor in lens homeostasis. (molvis.org)

A) Cell viability at 72 hours and (B) 6 days post-siCKAP5 treatment as measured by methoxynitrosulfophenyl-tetrazolium carboxanilide (XTT). (medicalxpress.com)
Values represent average % cell viability compared to cells treated with an equivalent concentration of siControl LNP. (medicalxpress.com)
BAY 11-7082 decreased cell viability, which was accompanied by caspase-3 cleavage. (lsd1receptor.com)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
These cells do not undergo apoptosis, and the primary outcome of caspase-2 activation is continued, cell division. (nature.com)
The tunicate Ciona intestinalis has a bi-phasic life cycle with swimming larvae which undergo metamorphosis after settlement. (biomedcentral.com)
In cells stimulated to undergo ferroptosis we identified PE-truncated species with sn-2 truncations ranging from 5 to 9 carbons. (cdc.gov)

We propose a model where caspase-2 regulates the cell cycle and protects DNA replication forks to prevent DNA damage accrual in a pathway distinct from its apoptotic role. (nature.com)
This suggests a non-apoptotic role for caspase-2 could drive its tumor suppression function. (nature.com)
However, whether caspase-2 plays an active role in regulating the cell cycle has up until now been unclear. (nature.com)
In our paper published in Oncogene [6], we show that caspase-2 plays an essential role in regulating normal cell division by protecting DNA replication forks from replication stress. (nature.com)
This indicates a role distinct from caspase-2's reported role in inducing apoptosis to remove damaged or aneuploid cells [7][8]. (nature.com)
therefore, we overexpressed Bcl-X L to distinguish caspase-2's role in cell division and DNA protection from its role in apoptosis. (nature.com)
This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
They frequently play an anti-apopto-tic role [14-16], but may also be pro-apoptotic in some cell types and conditions [16-19]. (lsd1receptor.com)
In this study we have investigated the role of NF-jB in fenretinide-induced cell death and the importance of NF-jB in ESFT. (lsd1receptor.com)
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. (affbiotech.cn)
In contrast to the apoptotic function of cell elimination, this new role of apoptosis is not well understood but seems caspase-dependent. (biomedcentral.com)
Here, we investigated the role of PGM1 and its regulation in CRC. (biomedcentral.com)
10) Therefore, it is important to evaluate its role in the cell cycle and its dysregulation. (ukessays.com)
In accordance with its tumor suppressor role, the Retinoblastoma protein pRb can ensure pro-apoptotic functions. (sdbonline.org)

Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)

We show that caspase-2 protects cells from a range of S-phase-related defects, including delayed exit from S-phase, increased stalled replication forks, increased new replication origins, and decreased repair of S-phase-associated chromosomal aberrations [6]. (nature.com)

Supports healthy inflammatory response via antioxidant and anti-apoptotic (prevention of cell death) properties (9). (alphascreed.com)

In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
In contrast, in cisplatin-resistant cells, BT-cisplatin combination treatment displayed synergistic effects at most of the drug ratios/concentrations. (biomedcentral.com)

If caspase-2 regulates cell division through the same pathway, it would be expected to phenocopy the cell cycle results of caspase-2 null cells. (nature.com)

E) Confocal microscopic images of Cy5siRNA-labeled LNP internalization in SKOV3, NAR, and A549 cells. (medicalxpress.com)

The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
Phosphatidylinositol-3-kinase (PI3-K) pathway has been reported as an important intracellular mediator frequently activated in cancer cells [ 2 ]. (biomedcentral.com)
Low doses of BT and cisplatin enhanced efficiency of cisplatin treatment in all the ovarian cancer cell lines tested. (biomedcentral.com)
In previous work, we showed that cancer cells do not depend on glycolysis for ATP production, but they do on fatty acid oxidation. (mdpi.com)
However, we found some cancer cells induced cell death after glucose deprivation along with a decrease of ATP production. (mdpi.com)
We investigated the different response of glucose deprivation with two types of cancer cells including glucose insensitive cancer cells (GIC) which do not change ATP levels, and glucose sensitive cancer cells (GSC) which decrease ATP production in 24 h. (mdpi.com)
One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
Melatonin has oncostatic properties in breast cancer cells. (explorationpub.com)
Additionally, maintenance of the light-dark cycle and oral supplementation of melatonin may have a beneficial effect on women who are facing the risk of breast cancer [ 12 ]. (explorationpub.com)
P73, P63 and Mutant P53: Members of Protein Complexs Floating in Cancer Cells -- P53: Gatekeeper, Caretaker or Both? (edu.au)
Here, we evaluated the efficacy of ST on three different human cancer cell lines including skin epidermoid carcinoma A431 cells, lung epithelial carcinoma A549 cells and breast adenocarcinoma MDA-MB-231. (biomedcentral.com)
This study provides valuable insight into the chemopreventive efficacy and associated molecular alterations of ST in breast cancer cells whereas it had only moderate efficacy on lung cancer cells and did not show any considerable effect on skin cancer cells. (biomedcentral.com)
Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)
99:209 -215.doi:10.1007/s11060 - 010 - 0129 -5 cell carcinoma-a population-based study in with and without cervical cancer in Tbilisi, Georgia. (who.int)

H460 rho(0) cells that lack mitochondrial DNA demonstrated a significant decrease in ROS production and apoptotic response to Cr(VI), indicating the involvement of mitochondrial ROS in Cr(VI)-induced apoptosis. (cdc.gov)

Basal levels of phosphorylated NF-jB were low in all ESFT cells. (lsd1receptor.com)
Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)

Anatomy26

Organisms9

Diseases6

Chemicals and Drugs122

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes75

Disciplines and Occupations1

Information Science3

Health Care1

Proliferation36

Proteins17

Differentiation5

Signaling pathway2

Arrest13

Pathways12

Mechanisms6

Proliferative8

Reactive oxygen3

Malignant3

Induces apoptosis1

Induce apoptosis2

Mitochondrial apoptosis1

Tumors4

Inhibition7

Survival7

Cyclin7

Progression2

Induction6

Hepatocellular carcinoma cell1

Inhibitor2

Migration4

Metabolism2

Vitro4

Overexpression2

Flow cytometry2

Chemotherapy2

Osteosarcoma cells1

Expression10

Immune8

Knockdown3

Transcription1

Genes5

Assay3

Mechanism3

Regulator3

Gene5

Epithelial cell1

Modulation1

Significantly5

Accumulation2

Growth5

Redox1

Viability3

Undergo4

Role15

Cleavage2

Defects1

Antioxidant1

Contrast2

Regulates cell1

Internalization1

Cancer15

Decrease1

Basal2