Antisense rna oligonucleotide. Medical search. Frequent questions

They include coding mRNAs and non-coding (nc) RNAs among them antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), micro-RNAs (miRNAs), small activating RNAs (saRNAs), RNA aptamers and RNA guides. (frontiersin.org)
There are currently several products containing RNA on the market, and many are under development, among them mRNA, antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), small activating RNAs (saRNAs), micro-RNAs (mi-RNAs), RNA aptamers and RNA guides. (frontiersin.org)
Antisense oligonucleotides (ASOs), defined as short synthetic oligonucleotides with single-stranded sequences complementary to certain mRNA sites, have been under drug development for approximately 30 years, with the first FDA-approved drug, Fomivirsen, being approved in 1998 ( Stein and Castanotto, 2017 ). (aspetjournals.org)
Common types of oligonucleotide therapeutics include antisense oligonucleotides (ASOs), small interfering RNAs. (thermofisher.com)
For this purpose, the researchers used small chemically-synthesized RNAs called Antisense Oligonucleotides (ASOs), which bind to the lncRNAs they target and lead to their degradation. (scitechdaily.com)
These include the use of small molecule inhibitors, antisense oligonucleotides (ASOs), antibody immunotherapy (anti-DPRs), and "bait" RNAs. (portlandpress.com)

Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and thereby blocks its translation into protein. (wikipedia.org)
While the first mRNA vaccines against an infectious disease have reached the market ( 1 , 2 ), many other medicinal products with RNA as an active substance (see Table 1 ), either vaccines against non-infectious diseases or non-vaccine drugs, are under development ( 3 - 18 ) or even approved ( 19 - 25 ). (frontiersin.org)
In many of these techniques, the pieces of RNA interact with a molecule called messenger RNA (or mRNA for short). (medlineplus.gov)
RNAi is an antisense mechanism that involves using RNA to target a specific messenger RNA sequence (mRNA) for degradation. (news-medical.net)
The antisense RNA within active RISC binds to its mRNA target, which is then cleaved and degraded by the RNase enzyme in the complex. (news-medical.net)
Based on the type, the RNA therapeutics market is segmented into RNA interference (RNAi) therapeutics, mRNA therapeutics, antisense oligonucleotide (ASO) therapeutics, and other therapeutics. (emailwire.com)
Therapeutic oligonucleotides, such as antisense oligonucleotides or ribozymes, are short segments of DNA that have been designed to hybridize to a sequence on a specific mRNA. (justia.com)
Moreover, as the word 'coding' refers to the relationship between nucleic acids and proteins, rather than the mere transcription of DNA into RNA, it is logical to call the strand with the mRNA sequence the coding strand, as in the first example. (genomicglossaries.com)
The antisense strand is the template for mRNA synthesis. (genomicglossaries.com)

Synthetic RNA is widely used in research applications as an important tool for gene functional analysis and the development of novel therapeutic strategies. (genscript.com)
We are pleased with the continued support from the NIH in our ongoing efforts to apply our RNA technologies, oligonucleotide chemistries and expertise to exploit antisense mechanisms, such as RNAi, for therapeutic benefit. (news-medical.net)
Thermo Scientific DNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. (thermofisher.com)
Read about: Oligo synthesis Oligo usage in diagnostics Oligo usage in therapeutics Phosphoramidites in oligo manufacturing Phosphoramidite considerations for diagnostic applications Phosphoramidite considerations for therapeutic applications, Oligonucleotides (oligos) are short pieces of DNA and RNA. (thermofisher.com)
Browse through our curated selection of learning resources to enable your success in oligonucleotide synthesis for diagnostic and therapeutic development. (thermofisher.com)
Mesyl phosphoramidate backbone modified antisense oligonucleotides targeting miR-21 with enhanced in vivo therapeutic potency. (yale.edu)
The GMAB technology platform, exclusively licensed from Yale University, utilizes a proprietary, cell-penetrating antibody to non-covalently bind to and deliver therapeutic levels of multiple types of nucleic acids, including synthetic RNA (ribonucleic acid) messenger RNA, small interfering RNA, deoxyribonucleic acids, antisense oligonucleotides and gene editing molecules. (biospace.com)
The preclinical results presented today highlight the ability of GMAB D31N to systemically deliver immunostimulatory RNAs in a targeted, non-invasive manner," said Peter M. Glazer, M.D., Ph.D., Chair of the Department of Therapeutic Radiology, Professor of Genetics and Robert E. Hunter Professor of Therapeutic Radiology at the Yale School of Medicine. (biospace.com)
On the other hand, the discontinuation/recalls of RNA therapeutic products is expected to hinder market growth. (emailwire.com)
Consequently, therapeutic oligonucleotides have tremendous potential for specificity of action (i.e. the down-regulation of a specific disease-related protein). (justia.com)
One well known problem with the use of therapeutic oligonucleotides having a phosphodiester internucleotide linkage is its very short half-life in the presence of serum or within cells. (justia.com)
Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
Background Therapeutic targeting of RNA may be a strategy which could inhibit the translation of one or more disease-associated proteins. (sbir.gov)
however, these technologies rely on derivatized oligonucleotide structures which have poor cell permeability and biodistribution which limits their effectiveness as a therapeutic. (sbir.gov)
These include RNA therapeutics that can partially or completely turn off the expression of disease-causing genes (antisense and interfering RNAs), help replace or supplement dysfunctional or insufficiently produced proteins (mRNAs), are developed as vaccines for cancer and infectious diseases, or either deliver sensitive therapeutic payloads or function as chemical drugs themselves by directly binding and inhibiting a target molecule (RNA aptamers). (harvard.edu)

Isis Pharmaceuticals, Inc. has announced that it has been awarded a multi-year Phase 2 Small Business Innovation Research (SBIR) grant by the National Institutes of Health (NIH) for up to $1.5 million to design oligonucleotide drugs that can exploit the RNA interference (RNAi) antisense mechanism for disease treatment. (news-medical.net)
Considering the complexity of MDM2 functions, we have chosen to use gene silencing technologies including antisense oligonucleotides and RNA interference. (nih.gov)
The treatment of these diseases depends on the underlying cause and may include immunotherapy, mitigation of risk factors, symptomatic treatment, and gene therapy, such as the recently developed RNA interference and antisense oligonucleotide therapies for transthyretin familial amyloid polyneuropathy. (bmj.com)
The RNA interference (RNAi) therapeutics segment is expected to grow at the highest CAGR from 2023 to 2028. (emailwire.com)
Factors associated with RNA interference (RNAi) therapeutics such as precision and versatility have increased the potential of RNAi applications in personalized medicine and other clinical applications. (emailwire.com)

View available technical assets related to using phosphoramidites in the development of oligonucleotide diagnostics and therapeutics. (thermofisher.com)
Oligonucleotide therapeutics are an up-and-coming class of drugs comprised of short DNA and/or RNA molecules that modulate the expression of target RNA for the treatment of diseases. (thermofisher.com)
Linker phosphoramidites provide options to link oligonucleotides to additional groups (e.g., chemical delivery system for therapeutics, fluorescent dyes, amines, phosphates) or attach to a solid surface like a DNA chip. (thermofisher.com)
Oligonucleotide therapeutics have attracted much attention recently. (thermofisher.com)
Growth in this market is largely driven by factors such as the increasing number of partnerships and collaborations among market players and RNA technology manufacturers, expanding modalities for RNA therapeutics, and the rising number of emergency use authorizations and approvals for COVID-19 booster vaccines. (emailwire.com)
The emergence of small activating RNAs (saRNAs) and circular RNAs (circRNAs) has expanded the scope of RNA therapeutics. (emailwire.com)
The vaccines segment accounted for the largest share of the RNA therapeutics market in 2022. (emailwire.com)
Based on product, the RNA therapeutics market is segmented into drugs and vaccines. (emailwire.com)
Increase in the development of innovative RNA medicine companies in the RNA therapeutics market has rendered it the fastest growing segment during the forecast period. (emailwire.com)
Based on the indication, the RNA therapeutics market is segmented into infectious diseases, rare genetic diseases/hereditary diseases, and other indications. (emailwire.com)
Increase in the use of RNA-based therapeutics for the treatment of rare/genetic disorders contributes to the high share of hospitals & clinics. (emailwire.com)
The RNA therapeutics market is segmented into hospitals and clinics and research settings. (emailwire.com)
The growing use of RNA therapeutics across hospitals and clinics is driven by factors such as the increasing understanding and advancements of RNA-based therapeutics, the growing number of trends in personalized medicine, the increasing prevalence of chronic diseases, and the rising research investments by governments, private foundations, and industry stakeholders. (emailwire.com)
Europe dominates the global RNA therapeutics market throughout the forecast period. (emailwire.com)
The global RNA therapeutics market is segmented into four major regions, namely, North America, Europe, the Asia Pacific, and the Rest of the World. (emailwire.com)
Current methods often yield suboptimal amounts and purities of the final product, and are limited by sequence length and the ability to efficiently incorporate non-standard nucleobases common to many RNA therapeutics. (harvard.edu)
In addition, current methods use large quantities of environmentally harmful solvents as well as lengthy purification steps that drastically increase the overall cost of RNA oligonucleotide synthesis, especially at the scales needed for wide distribution of high-quality therapeutics to patients. (harvard.edu)
Furthermore, these limitations also prevent researchers from creating novel RNA-based therapeutics in the first place. (harvard.edu)
Importantly, this level of control also enables the introduction of modified oligonucleotides - many of which thus far have been inaccessible as building blocks for RNA therapeutics - at defined positions. (harvard.edu)
In recent years, successful results have been obtained in the treatment of genetic diseases, cancer immunotherapy and against emerging infectious diseases with nucleic acid-based therapeutics, especially small interfering RNAs (siRNAs) and antisense oligonucleotides. (hamiltoncompany.com)
Stoke Therapeutics, Inc. (Nasdaq: STOK), a biotechnology company dedicated to addressing the underlying cause of severe diseases by upregulating protein expression with RNA-based medicines, today reported financial results for the first quarter of 2022 and provided business updates. (financialcontent.com)

The Phase 2 grant builds upon a successfully completed Phase 1 program that demonstrated the feasibility of using single-stranded antisense drugs to target the RNAi pathway. (news-medical.net)
Much of the work will focus on optimizing the chemical properties of single-stranded oligonucleotides that trigger the RNAi pathway. (news-medical.net)
This provides the opportunity to develop simplified single-stranded antisense drugs that can utilize the RNAi pathway to treat human disease. (news-medical.net)
Most importantly, when using a mouse B16 melanoma tumor model, substantial tumor growth suppression was observed upon intravenous injection of GMAB D31N non-covalently bound to 3pRNA, an 89-nucleotide, single-stranded RNA. (biospace.com)

An RNA therapy called RNA aptamer therapy introduces small pieces of RNA that attach directly to proteins to alter their function. (medlineplus.gov)
The most common RNAi agents are double-stranded RNAs, which ultimately associate with proteins to form an RNA-induced silencing complex (RISC). (news-medical.net)
As the name long noncoding RNAs implies, unlike messenger RNAs (mRNAs), they do not encode the construction plans for proteins. (scitechdaily.com)
Performing proof-of-concept studies to demonstrate that small molecule binding to HIV RNA can prevent processing and translation into proteins in relevant cell lines and primary cells. (sbir.gov)

Examples of these RNA therapies include antisense oligonucleotide (ASO), small interfering RNA (siRNA), and microRNA (miRNA) therapies. (medlineplus.gov)
Here we report the discovery and development of olpasiran, a first-in-class, synthetic, double-stranded, N -acetylgalactosamine-conjugated small interfering RNA (siRNA) designed to directly inhibit LPA messenger RNA translation in hepatocytes and potently reduce plasma Lp(a) concentration. (nature.com)

Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. (cdc.gov)
We describe here a new class of caged morpholino oligonucleotides that can be activated by the bacterial nitroreductase NfsB. (cdc.gov)
1 In particular, morpholino oligonucleotides (MOs) have been used to block the expression of targeted genes in several invertebrate and vertebrate models. (cdc.gov)
Antisense morpholino oligonucleotides directed at intronic pseudoexons have been shown to increase propionyl-CoA carboxylase activity to normal levels in fibroblast cell lines derived from patients suffering from propionic acidemia. (medscape.com)

The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
The identification of detailed RNA structures now allows the design of small molecules which are capable of binding to RNA with high selectivity and specificity. (sbir.gov)
Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)

GenScript provides high-quality and cost-effective custom RNA oligos with flexible synthesis scales to meet researchers' different needs. (genscript.com)
The idea of asRNAs as drug targets started in 1978 when Zamecnik and Stephenson found an antisense oligonucleotide to the viral RNA of Rous scarcoma virus that was capable of inhibiting viral replication and protein synthesis. (wikipedia.org)
Phosphoramidites (often abbreviated as amidites) are building blocks used in chemical synthesis of oligonucleotides. (thermofisher.com)
Dye-labeled phosphoramidites are intended for oligonucleotide synthesis designed for molecular assays, microscopy, and microarrays. (thermofisher.com)
Succinates are used for the derivatization of controlled pore glass, polystyrene, or other supports for solid-phase oligonucleotide synthesis. (thermofisher.com)
The oligonucleotide synthesis market is expected to register a CAGR of 12.3% over the forecast period. (mordorintelligence.com)
COVID-19 has impacted the growth of the oligonucleotide synthesis market. (mordorintelligence.com)
Moreover, various companies are investing in oligonucleotide synthesis, which is expected to increase market growth over the forecast period. (mordorintelligence.com)
Despite the rapidly growing market and increasing demand for RNA oligonucleotides, their production still primarily relies on antiquated chemical synthesis methods. (harvard.edu)
A research team in the Wyss Institute's Synthetic Biology platform lead by George Church, Ph.D., is developing a new enzymatic-based, template-independent RNA oligonucleotide synthesis technology (eRNA) to address the current limitations of traditional chemical synthesis. (harvard.edu)
Their scalable, flexible, and cost-efficient synthesis method uses a set of proprietary engineered enzymes and novel nucleotide building blocks to produce accurate, high quality RNA oligonucleotide sequences comprised of natural and non-natural bases at efficiencies that diminish the need for post-reaction purification. (harvard.edu)
In addition to providing a substantially "greener" approach to oligonucleotide synthesis via aqueous reaction conditions, this technology could potentially enable the synthesis of highly customized and significantly longer RNA oligonucleotides. (harvard.edu)
Current chemical synthesis techniques can synthesize RNA oligonucleotides up to 120 nucleotides, but for extreme costs and at small scales. (harvard.edu)
With a newly developed blocking strategy that allows the synthesis system to tightly control nucleotide additions at each step of the growing RNA oligonucleotide chain, the overall accuracy of synthesis is greatly increased - conventional methods are prone to the accumulation of premature truncation products, nucleobase depurination, and insertions/deletions. (harvard.edu)
In contrast, existing some RNA synthesis methods introduce a modified nucleotide, like for example a modified A nucleotide at all A-specific positions of the growing RNA oligonucleotide sequence. (harvard.edu)

In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
Many scientists are still confused by the terms sense and antisense when referring to DNA because the terminology has changed over the years. (genomicglossaries.com)

Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
Targets for these drugs include the genes or RNA products of c-myc, ICAM-1, and infectious disease organisms such as cytomegalovirus, and HIV-1. (justia.com)
They searched for novel targets in the poorly understood class of genes known as "long noncoding RNAs (Ribonucleic acids)" (lncRNAs). (scitechdaily.com)
We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
Los ARNs antisentido sintéticos se utilizan para afectar el funcionamiento de genes específicos en la investigación o con fines terapéuticos. (bvsalud.org)

saRNAs are 21-nucleotide long double-stranded RNAs (dsRNAs) complementary to the promoter region of a targeted gene. (emailwire.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (bvsalud.org)

What are CAR T cell therapy, RNA therapy, and other genetic therapies? (medlineplus.gov)
Several techniques, called RNA therapies, use pieces of RNA, which is a type of genetic material similar to DNA, to help treat a disorder. (medlineplus.gov)
Synthetic RNA oligonucleotides designed as specific successions of the four nucleobases A, U, G, and C that mimic naturally occurring RNA species are the key components of diverse RNA-based therapies. (harvard.edu)

The centrality of RNA for engineering gene expression. (nih.gov)
Inactivating Gene Expression with Antisense Modified Oligonucleotides. (yale.edu)
Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. (nature.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (bvsalud.org)

The regions that are predicted to have conserved RNA structures and act as orphan promoters and Rho independent terminators are preferenced during analysis. (wikipedia.org)

Antisense mixed-backbone oligonucleotides (MBO) specifically inhibit MDM2 expression in a dose- and time-dependent manner, resulting in significant anti-tumor activity in vitro and in vivo. (nih.gov)

Although most currently practiced methods of inducing the RNAi mechanism utilize double-stranded RNAs, RISC requires only the antisense strand to function. (news-medical.net)

By developing small molecules to selectively bind to key sites on transcribed HIV RNA the translation of RNA to protein may be inhibited for any HIV intracellular protein. (sbir.gov)
Targeting one or more HIV RNA sequences with small molecules may be an effective way of shutting down viral replication, preventing cellular transmission and ultimately leading to sustained viral remission. (sbir.gov)
Project Goal The goal of this SBIR solicitation is to support the discovery and design of RNA-targeted small molecules which specifically bind to HIV RNA transcripts to prevent RNA processing and translation into protein. (sbir.gov)
Designing, optimizing and testing strategies for the targeting of small molecules to key sites on HIV RNA. (sbir.gov)

Many RNA-based drugs, both vaccines and non-vaccines, are under development or even approved. (frontiersin.org)
The arrival of anti-Covid-19 RNA vaccines in 2020 should not obscure the fact that for several years we have already had treatments based on interfering RNA or antisense oligonucleotides in a number of rare diseases with a very poor prognosis such as transthyretin amyloidosis, acute hepatic porphyria, primary hyperoxaluria, spinal muscular atrophy or familial hyperchylomicronemia. (academie-medecine.fr)
Europe leads the market for RNA vaccines, with highest share of revenue in this segment in 2022. (emailwire.com)

The kit incorporates unique D-Plex technology for library preparation enabling high performance with ultralow RNA inputs and providing diverse small RNA transcript detection. (mordorintelligence.com)

Unlike micF RNA being discovered by accident, the majority of asRNAs were discovered by genome wide searches for small regulatory RNAs and by transcriptome analysis. (wikipedia.org)
But, in the competitive context of innovations in the field, interfering RNAs and antisense oligonucleotides will have to reckon with gene therapy and genome editing using the CRISPR-Cas 9 technique. (academie-medecine.fr)
In a study published in PLOS One in March 2022, it was observed that the oligonucleotides successfully targetted 5'-polyUs in the coronavirus genome. (mordorintelligence.com)
In the latest genome annotation, lncRNAs, which are arbitrarily defined as noncoding RNAs longer than 200 nucleotides, constitute ~72% of the transcribed genome 13 , whereas mRNAs comprise only 19%, indicating the need for functional annotation of lncRNAs. (nature.com)
In addition, RNA oligonucleotides are vastly deployed in genome editing technologies. (harvard.edu)

These DDR RNAs (DDRNAs) are essential for DDR activation: removal of DDRNAs by RNAse A treatment of permeabilized cells inhibits DDR activation and DDR can be fully restored by the addition of chemically-synthesized DDRNA carrying the sequence surrounding the damaged site but not other sequences (Francia, Nature 2012). (europa.eu)
We define sequences that regulate PE inclusion and protein expression of the oncogenic SF TRA2β using an RNA-targeting CRISPR screen. (mendeley.com)
When DNA sequences are described by giving the sequence of only one strand, this is usually the strand with the same sequence as the RNA (messenger, ribosomal, transfer, etc.) and should therefore be called the coding strand. (genomicglossaries.com)

We observed that DNA double-strand breaks (DSBs) trigger the local generation of small non-coding RNAs at the site of DNA damage carrying the sequence surrounding the damaged site. (europa.eu)

A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future. (nih.gov)

However, none of these solutions have proven entirely satisfactory, and in vivo free antisense still has only limited efficacy. (justia.com)

This strategy has been successfully from large groupings of organisms, electrospray ionization used with consensus 16S ribosomal RNA primers for deter- mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to mining bacterial diversity, both in environmental samples identify organisms in a sample. (cdc.gov)

Subsequent antisense oligonucleotide-knockdown transcriptomic profiling of two LEC- and two BEC-specific lncRNAs identifies LETR1 as a critical gatekeeper of the global LEC transcriptome. (nature.com)

Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize. (genomicglossaries.com)

The large share of the infectious diseases segment is a result of the rapid developments in RNA vaccine manufacturing capabilities for the treatment of infectious diseases along with the outbreak of COVID-19. (emailwire.com)

Cell culture studies show antibody-mediated delivery of a series of retinoic acid-inducible gene I (RIG-I) ligands, including hairpin RNAs and synthetic double-stranded RNA (poly(I:C)), resulting in robust RIG-I stimulation and induction of type-1 interferon signaling. (biospace.com)

L'arrivée des vaccins anti Covid-19 à ARN en 2020 ne doit pas occulter le fait que nous disposons déjà depuis plusieurs années de traitements à base d'ARN interférents ou d'oligonucléotides antisens dans un certain nombre de maladies rares de pronostic très sombre telles que l'amylose à transthyrétine, la porphyrie hépatique aiguë, l'hyperoxalurie primitive, l'amyotrophie spinale ou l'hyperchylomicronémie familiale. (academie-medecine.fr)

Antisense oligonucleotide gapmers containing phosphoryl guanidine groups reverse MDR1-mediated multiple drug resistance of tumor cells. (yale.edu)
Studies completed with labeled RNAs and messenger RNAs expressing a green fluorescent protein reporter gene show tumor specific delivery and functional expression by imaging, with minimal delivery to healthy tissues. (biospace.com)
We were positively surprised to see how well the antisense oligonucleotides could restrain tumor growth in different models," states Taisia Polidori, co-first author, who worked on the project as part of her doctoral thesis research at the University of Bern. (scitechdaily.com)

To detect asRNA transcribed from the encoding region, oligonucleotide microarrays can be used. (wikipedia.org)

STK-001 is a proprietary antisense oligonucleotide (ASO) being developed by Stoke as the first potential new medicine to address the genetic cause of Dravet syndrome. (yahoo.com)

To ensure that the RNA oligos are of highest quality, all RNA oligonucleotides are identified by ESI (electrospray ionization) mass spectrometry. (genscript.com)
Long RNA oligos up to 180 nt are now available! (genscript.com)
Our standard custom DNA oligonucleotides are synthesized on a highly automated, computer-controlled system using cyanoethyl phosphoramidite chemistry and subject to rigorous quality control procedures, just like our Value Oligos. (thermofisher.com)

We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)

The Medicines Manufacturing Innovation Centre launched a collaboration with AstraZeneca, Exactmer, Novartis, and UK Research and Innovation to develop a scalable, sustainable, and more cost-effective medicines manufacturing process for oligonucleotides: short strands of synthetic DNA or RNA. (mordorintelligence.com)

For example, in plasmid ColE1, the asRNA termed RNA I plays an important role in determining the plasmid copy number by controlling replication. (wikipedia.org)
Gene therapy involves viral, plasmid, and oligonucleotide-based approaches. (medscape.com)

Unmodified RNA, RNA with modifications, chimeric oligonucleotides with mixed DNA and RNA bases, 2'-OMe-RNA, 2'-F-RNA, and other antisense RNAs are available at your specific request. (genscript.com)
RNA modifications (2'-OMe-RNA etc.), modified bases (2-Aminopurine etc. (genscript.com)
1997. Antisense: Chemical Modifications. (justia.com)
Several MO caging strategies have been devised, employing hairpin structures, 7 intermolecular duplexes, 8 , 9 nucleobase modifications, 10 or oligonucleotide cyclization. (cdc.gov)

Effective treatment of spinal muscular atrophy with antisense oligonucleotide therapy opens the door to treating other neurological disorders with this approach. (ox.ac.uk)
RNA Therapy: Current Status and Future Potential. (medlineplus.gov)

Once RNA II is transcribed, it hybridizes to its DNA template and later cleaved by RNase H. In the presence of the asRNA RNA I, RNA I and RNA II forms a duplex which introduces a conformational change of RNA II. (wikipedia.org)

This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)

For instance, in September 2021, SpeeDx invested in local oligonucleotide manufacturing to reduce supply chain risk and develop a more vital sovereign capacity for critical diagnostics. (mordorintelligence.com)
For instance, in February 2021, CPI launched a project in the Medicines Manufacturing Innovation Centre to revolutionize the manufacture of oligonucleotides. (mordorintelligence.com)
In January 2021, Diagenode launched a new small RNA-sequencing solution, D-Plex Small RNA DNBSEQ Kit, compatible with MGI's DNBSEQ sequencers. (mordorintelligence.com)

pp147-161 in Gene Regulation: Biology of Antisense RNA and DNA (Eds. (justia.com)

These results provide a basis for clinical evaluation of antisense anti-MDM2 oligonucleotides as chemosensitizers and radiosensitizers. (nih.gov)
Other significant factors include the increasing government investments and R&D expenditure in pharmaceutical and biotechnology companies and the use of synthesized oligonucleotides in molecular diagnostics and clinical applications. (mordorintelligence.com)
Furthermore, the increasing adoption of oligonucleotides in molecular diagnostics and clinical applications contributes to market growth. (mordorintelligence.com)
Advanced human clinical assessments of several antisense drugs are currently underway. (justia.com)

1993. Inhibition of HIV-1 Replication in Cultured Cells with Antisense Oligonucleotides Encapsulated in Immunoliposomes. (justia.com)

Structural, nonstandard phosphoramidites are used to synthesize modified oligonucleotides. (thermofisher.com)

The resulting blockade of RNA splicing or translation allows loss-of-function phenotypes to be determined within days, contrasting the months that can be required to obtain homozygous mutants. (cdc.gov)
We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)

The increased demand for COVID-19 diagnostics has, in turn, increased the global demand for oligonucleotide raw materials that form the basis of molecular diagnostic products. (mordorintelligence.com)
Modified nucleotide building blocks can stabilize RNA oligonucleotide products, uniquely endow RNA oligonucleotides with functional properties, and facilitate delivery to their molecular targets within the body's tissues. (harvard.edu)

The RNA isoform repertoire is regulated by splicing factor (SF) expression, and alterations in SF levels are associated with disease. (mendeley.com)

Anatomy6

Organisms4

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment19

Phenomena and Processes44

Technology, Industry, Agriculture1

Information Science3

ASOs6

MRNA9

Therapeutic15

Interference5

Therapeutics21

Single stranded4

Proteins4

SiRNA2

Morpholino oligonucleotides4

Specificity4

Synthesis15

Sense and antisense2

Genes5

Complementary2

Therapies3

Gene expression4

Conserved RNA structures1

Mixed-backbone1

RNAi1

Molecules4

Vaccines3

Transcript1

Genome5

Sequences3

Sequence1

Synthetic biology1

Vivo1

Ribosomal1

Transcriptome1

Fragments1

Diseases1

Double-stranded1

20201

Tumor3

Microarrays1

Genetic1

Oligos3

LncRNAs1

Short1

Plasmid2

Modifications4

Therapy2

Conformational1

Structures1

20213

Regulation1

Clinical4

Inhibition1

Synthesize1

Function2

Molecular2

Disease1