ASOsInjection of antisense oligonucleotidesSynthetic oligonucleotidesTherapeuticTherapyRNAsDegradationVitroSiRNAs2019SynthesisMRNAComplementaryInduceSequenceShown to decreaseToxicityMoleculesProteinsGene expressionSubsequentModificationsReductionCleavageAutoradiographyMutantProteinGenesAssaysMiceMedicineStudyEffectsEffectiveDeliveryCellsModelsDrugTarget
ASOs7
- Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)
- Antisense oligonucleotides (ASOs), defined as short synthetic oligonucleotides with single-stranded sequences complementary to certain mRNA sites, have been under drug development for approximately 30 years, with the first FDA-approved drug, Fomivirsen, being approved in 1998 ( Stein and Castanotto, 2017 ). (aspetjournals.org)
- Antisense oligonucleotides (ASOs) offer a new therapeutic strategy in Parkinson's disease (PD) because they can be readily targeted to genes causally linked to PD development or progression. (shakeitup.org.au)
- Antisense oligonucleotides (ASOs) are versatile agents for controlling the translation and splicing of mRNA. (elsevierpure.com)
- A novel approach to tritium-labeled antisense oligonucleotides (ASO) was established by conjugating N -succinimidyl propionate, as well as maleimide-derivatives, to the 3′-end of ASOs targeting metastasis-associated lung adenocarcinoma transcript 1 (Malat1) containing amino- or sulfhydryl-linkers. (springeropen.com)
- They include coding mRNAs and non-coding (nc) RNAs among them antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), micro-RNAs (miRNAs), small activating RNAs (saRNAs), RNA aptamers and RNA guides. (frontiersin.org)
- There are currently several products containing RNA on the market, and many are under development, among them mRNA, antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), small activating RNAs (saRNAs), micro-RNAs (mi-RNAs), RNA aptamers and RNA guides. (frontiersin.org)
Injection of antisense oligonucleotides1
- Pilot studies in the YAC128 transgenic HD mouse model revealed skipping of human huntingtin exon 12 after a single intracerebroventricular injection of antisense oligonucleotides. (bmj.com)
Synthetic oligonucleotides1
- Synthetic Oligonucleotides are poised for explosive growth as gene technology moves into mainstream healthcare, food production and, even, Data Storage! (pharmiweb.com)
Therapeutic3
- Therapeutic Oligonucleotides target the mRNA and change gene expression (proteins). (artofhealingcancer.com)
- Many antisense therapeutic drugs are available in the market and are being used for the treatment of multiple disorders like cancer, Alzheimer's disease, Parkinson's disease, and arthritis. (artofhealingcancer.com)
- Gendicine was the first antisense therapeutic drug that was approved for head and neck cancer in China. (artofhealingcancer.com)
Therapy8
- Antisense oligonucleotide (ASO) therapy has the potential to ameliorate many neurodegenerative diseases at the genetic level to suppress the production of harmful proteins or non-coding RNAs. (news-medical.net)
- Antisense oligonucleotide therapy plays a vital role in cancer and genetic disorders. (artofhealingcancer.com)
- This therapy involves the use of oligonucleotides that are complementary to specific target DNA or RNA in the nucleus. (artofhealingcancer.com)
- The use of oligonucleotides that are complementary to our target mRNA in order to reduce the expression of some specific gene in a cell is called antisense therapy. (artofhealingcancer.com)
- So, oligonucleotides used for antisense therapy should be modified in such a way that they have high stability and low side effects. (artofhealingcancer.com)
- The use of oligonucleotides for the treatment of cancer patients is found to be a very effective therapy. (artofhealingcancer.com)
- Antisense therapy is effective in the sense that it can distinguish between the normal and mutated oncogenes in cells and targets only mutated oncogenes without downregulating the expression of normal genes. (artofhealingcancer.com)
- Effective treatment of spinal muscular atrophy with antisense oligonucleotide therapy opens the door to treating other neurological disorders with this approach. (ox.ac.uk)
RNAs2
- Unmodified RNA, RNA with modifications, chimeric oligonucleotides with mixed DNA and RNA bases, 2'-OMe-RNA, 2'-F-RNA, and other antisense RNAs are available at your specific request. (genscript.com)
- This can be done by chemically modifying the oligonucleotides like CpG, anti-gene, small non-coding RNAs, etc. (artofhealingcancer.com)
Degradation4
- The research team had recently developed DNA/RNA heteroduplex oligonucleotide (HDO) technology capable of highly efficient RNA degradation in vivo. (news-medical.net)
- Site-specific degradation of DNA was achieved by the use of DNA oligonucleotides covalently tethered to phenazine 5,10-di-N-oxide. (elsevierpure.com)
- Several parameters of DNA degradation were studied, including the effect on DNA degradation of chain length in the tether connecting the oligonucleotides and prosthetic group, the relative efficiencies of DNA cleavage when the prosthetic group was in the middle or at the end of the antisense oligonucleotide, and the effect of O 2 on DNA degradation. (elsevierpure.com)
- Also studied was the actual chemistry of DNA oligonucleotide degradation and the ability of individual diastereomers of the modified oligonucleotides to mediate degradation of the target DNA. (elsevierpure.com)
Vitro1
- Using viral RNA, Stephenson and Zamecnik ( 1978 ) demonstrated in 1978 that translation of proteins could be efficiently inhibited in vitro by competitive hybridization with antisense oligonucleotides (ASO). (springeropen.com)
SiRNAs2
- JHEP Live: Antisense oligonucleotides vs. siRNAs in the treatment of chronic hepatitis B: Who will be the winner, if at all? (easl.eu)
- In this JHEP Live the faculty will be discussing the use of antisense oligonucleotides and siRNAs in the treatment of chronic hepatitis B infections. (easl.eu)
20191
- Liu, Z & Corey, DR 2019, CHAPTER 2: Mechanisms of Antisense Oligonucleotides . (elsevierpure.com)
Synthesis7
- Oligonucleotides bind the complementary sequence and inhibit the synthesis of specific proteins by different mechanisms. (artofhealingcancer.com)
- The oligonucleotide sequence binds the mRNA in the cell and prevents the synthesis of specific proteins from it. (artofhealingcancer.com)
- In the present study, we have investigated the involvement of clusterin in cell-substratum interaction by the inhibition of clusterin synthesis using antisense oligonucleotide. (korea.ac.kr)
- Treatment of antisense oligonucleotide induced a marked reduction of synthesis for clusterin protein, but not for clusterin mRNA expression, suggesting the translational suppression of clusterin by antisense oligonucleotide. (korea.ac.kr)
- Oligonucleotide Synthesis Market Report 2022: The RNA Bas. (pharmiweb.com)
- DUBLIN--(BUSINESS WIRE)--The "Oligonucleotide Synthesis Markets - Forecasts by Oligo Length, Application, and Product - With Executive and Consultant Guides and including Customized Forecasting and Analysis" report has been added to ResearchAndMarkets.com's offering. (pharmiweb.com)
- 2.1 What is Oligonucleotide Synthesis? (pharmiweb.com)
MRNA5
- They comprise a few dozen base pairs arranged in an 'antisense' or reverse order and prevent production of pathogenic proteins through binding to the 'sense' strand of mRNA targets. (news-medical.net)
- These oligonucleotides bind in the 5 to 3 directions with mRNA and do not allow it to translate into proteins. (artofhealingcancer.com)
- modalities because oligonucleotides can easily be modified, they have a high affinity to bind targeted mRNA, they are highly specific, and have no side effects. (artofhealingcancer.com)
- This prevention may result when oligonucleotides cause inhibition of the translation process, they may prevent the spicing of exons, or they inhibit the transport of mRNA from the nucleus into the cytoplasm which is important for translation to occur. (artofhealingcancer.com)
- Transfection of ASC-17D Sertoli cells with a 20-base antisense oligonucleotide against clusterin mRNA resulted in extracellular release of LDH and DNA fragmentation. (korea.ac.kr)
Complementary4
- The chemical modifications allow the oligonucleotides to enter the nucleus and bind their complementary RNA which is also an RNA of interest. (artofhealingcancer.com)
- The oligonucleotides are of specific sequences complementary to the target RNA or DNA sequence. (artofhealingcancer.com)
- So, there is no chance of multiple complementary sites for oligonucleotides in the genome and hence no harm to normal cells. (artofhealingcancer.com)
- When annealed to a complementary DNA target strand, the antisense oligonucleotide effected alkylation of guanosine residues in proximity to the phenazine di-N-oxide prosthetic group. (elsevierpure.com)
Induce1
- Using antisense oligonucleotides, we induce skipping of huntingtin exon 12 that encodes important cleavage sites. (bmj.com)
Sequence1
- Sertoli cell death by antisense oligonucleotide of clusterin was sequence specific and dose dependent. (korea.ac.kr)
Shown to decrease2
- Using the P-S mutation was shown to decrease the Tm of the oligonucleotide, which leads to a lower target affinity. (wikipedia.org)
- Antisense oligonucleotides (AO) have been shown to decrease apo(a) protein production in hepatocytes, which is the primary source of circulating Lp(a). (pace-cme.org)
Toxicity1
- While recent publications have shown promising results reducing mutant huntingtin toxicity by lowering huntingtin levels using antisense oligonucleotides, there is some concern regarding unwanted side effects caused by lowering huntingtin protein levels too much. (bmj.com)
Molecules1
- For larger molecules such as oligonucleotides, however, the resulting molar specific activity after carbon-14 (62.4 mCi/mmol) labeling might be insufficient for a quantitative determination by liquid scintillation counting (LSC). (springeropen.com)
Proteins2
- These are Oligonucleotides which cuts the expression of proteins with in a cell. (artofhealingcancer.com)
- Understanding the miRNA sequences involved in these diseases can allow us to use anti miRNA Oligonucleotides to disrupt pathways that lead to the under/over expression of proteins of cells that can cause symptoms for these diseases. (wikipedia.org)
Gene expression1
- In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
Subsequent1
- Subsequent antisense oligonucleotide-knockdown transcriptomic profiling of two LEC- and two BEC-specific lncRNAs identifies LETR1 as a critical gatekeeper of the global LEC transcriptome. (nature.com)
Modifications1
- During anti-miRNA oligonucleotide design, necessary modifications to optimize binding affinity, improve nuclease resistance, and in vivo delivery must be considered. (wikipedia.org)
Reduction1
- Moreover, the target population for future trials on Lp(a) reduction with antisense oligonucleotides is currently hotly debated. (pace-cme.org)
Cleavage1
- Nagai, K & Hecht, SM 1991, ' Site-specific DNA cleavage by antisense oligonucleotides covalently linked to phenazine Di-N-oxide ', Journal of Biological Chemistry , vol. 266, no. 35, pp. 23994-24002. (elsevierpure.com)
Autoradiography1
- In conclusion, this new method provides a powerful technique for fast and safe access to tritium-labeled oligonucleotides, e.g., for pharmacokinetic, mass balance, or autoradiography studies. (springeropen.com)
Mutant2
- They then tested oligonucleotides on two lines of mice genetically engineered to have problems associated with SCA2 by programming neurons in the cerebellum to make mutant ataxin 2. (sciencebeta.com)
- Antisense downregulation of mutant huntingtin in a cell model. (biomedcentral.com)
Protein1
- Attenuation of matrix protein secretion by antisense oligodeoxynucleotides to the cyclin kinase inhibitor p21(Waf1/Cip1). (biomedcentral.com)
Genes2
- When oligonucleotide-based drugs are given to the cancer patient, the drugs target the specific mutated genes in cancerous cells. (artofhealingcancer.com)
- We will focus on the use of antisense oligonucleotides, cell cycle analysis, and luciferase reporter genes. (biomedcentral.com)
Assays1
- The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
Mice2
- We have recently started a follow-up study to assess the longitudinal phenotypical effect of antisense oligonucleotide-mediated exon skipping in YAC128 HD mice. (bmj.com)
- Compared to placebo, injections of the antisense oligonucleotides into the nervous system of the newborn mice extended their median lifespan by 35 percent and improved their ability to walk, while lowering ataxin 2 gene levels in the brain and spinal cord. (sciencebeta.com)
Medicine1
- The market for small snippets of DNA, called Oligonucleotides, is growing into a market for Synthetic DNA where organisms, like algae, are "custom designed" to produce food and medicine while consuming few resources. (pharmiweb.com)
Study1
- The current study describes the implementation of a hybridization liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform for the direct quantitation of antisense oligonucleotide (ASO) ƒ u . (aspetjournals.org)
Effects1
- The oligonucleotides that are available naturally are usually unstable or with low stability and efficacy as they have a lot of side effects. (artofhealingcancer.com)
Effective2
- In both lines, the oligonucleotides appeared to be effective. (sciencebeta.com)
- A subline of the human B cell lymphoma DHL-4, grown in the artificial serum-free medium HB101, displayed a resistant phenotype to the activity of an antisense oligodeoxynucleotide (aODN) effective on the parental DHL-4 line. (mcmaster.ca)
Delivery1
- Gewirtz AM, Stein CA, Glazer PM. Facilitating oligonucleotide delivery: helping antisense deliver on its promise. (biomedcentral.com)
Cells1
- Further, microscopic observation showed that more noticeable cell death was induced by treating the antisense prior to plating the cells than by treating after cell attachment to the plate. (korea.ac.kr)
Models1
- Scn8a antisense oligonucleotide is protective in mouse models of SCN8A Encephalopathy and Dravet Syndrome. (oligotherapeutics.org)
Drug2
- The type of drug they used is called an antisense oligonucleotide. (sciencebeta.com)
- An antisense oligonucleotide drug has been approved by the Food and Drug Administration for treating spinal muscular atrophy, a hereditary disorder that causes arm and leg muscle weakness and deterioration in children. (sciencebeta.com)
Target1
- the latter effected strand scission of the target DNA oligonucleotide. (elsevierpure.com)