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SilverSilver NitrateAntigensSilver CompoundsSilver StainingAntigens, BacterialAntigens, NeoplasmAntigens, SurfaceSilver SulfadiazineAntigens, ViralMetal NanoparticlesAntigens, ProtozoanAntigens, Polyomavirus TransformingHLA AntigensAntigens, FungalAntigens, CDAntigens, HelminthH-2 AntigensCarcinoembryonic AntigenAntigens, Viral, TumorHLA-DR AntigensReceptors, Antigen, T-CellAntibodies, MonoclonalHistocompatibility AntigensProliferating Cell Nuclear AntigenArgyriaO AntigensHistocompatibility Antigens Class IIEpitopesGreen Chemistry TechnologyProstate-Specific AntigenReceptors, Antigen, B-CellAntigens, CD15Antigens, Tumor-Associated, CarbohydrateHLA-A2 AntigenAntigens, CD8T-LymphocytesEnzyme-Linked Immunosorbent AssayImmunoglobulin GSilver ProteinsCross ReactionsBlood Group AntigensHepatitis B Surface AntigensAntigens, CD3Molecular Sequence DataHLA-A AntigensMice, Inbred BALB CHistocompatibility Antigens Class IAntibody SpecificityLymphocyte ActivationHLA-D AntigensReceptors, AntigenFluorescent Antibody TechniqueAntigens, CD45Hepatitis B AntigensAntigens, CD4Immune SeraAntibodies, BacterialAntigen-Antibody ReactionsAmino Acid SequenceAntigens, CD1Cell LineAntibody FormationHLA-B AntigensAntigens, DifferentiationImmunizationStaining and LabelingB-LymphocytesMART-1 Antigengp100 Melanoma AntigenNucleolus Organizer RegionImmunoenzyme TechniquesMolecular WeightHIV AntigensAntigens, CD80Electrophoresis, Polyacrylamide GelEpstein-Barr Virus Nuclear AntigensMice, Inbred C57BLAntigens, CD19Base SequenceSpleenAntigens, HeterophileRabbitsAntibodiesHepatitis B Core AntigensCells, CulturedAntigens, CD40ImmunodiffusionAutoantigensImmunoglobulin MAntigens, Thy-1Forssman AntigenAntigen-Antibody ComplexOvalbuminH-Y AntigenAntigen-Presenting CellsDendritic CellsComplement Fixation TestsHLA-DQ AntigensFlow Cytometry
AntibodyAntibodiesMethenamine silver stainBiopsyGramTissueFontana-MassonGelsProteinGomoriDetectionSmearsUrineImpregnationImmunohistochemistryYeastPolyclonalFungiRetrievalReactiveEosinPeriodicCapsuleMethodsParaffinDiagnosticSlidesSuggestsProcedureCellSodiumPretreatmentReagentsAssayCounterstainImmuneLaboratoryTestTechniqueAdequateSpecificDensitySamplesShowSolutionExpressionPositiveProduction
Antibody10
  • The EcoRI-HindIII restriction fragment (AA: 479-558 fragment name hL1RT EH ) from the RT region was used as an antigen to make antibody against hL1ORF2p (shaded by yellow). (biomedcentral.com)
  • g Dialysed and concentrated hL1RT EH fragment protein (antigen) injected to mice for antibody generation. (biomedcentral.com)
  • h Silver staining of purified hL1RT EH (antigen) show the purity of antigen used to generate the antibody. (biomedcentral.com)
  • An immunogold-silver staining technique using the polyclonal antibody to M. sydneyi failed to identify the presumed presporulation stage of M. sydneyi in the connective tissue of a recently infected host. (alr-journal.org)
  • Culture and assay of serum or cerebrospinal fluid for capsular antigen and antibody (latex agglutination test) may help in diagnosis. (medscape.com)
  • After appropriate heat induced antigen retrieval with BOND Epitope Retrival Solution1 or 2, sections were incubated with the primary monoclonal antibody against the CD68 (clone PGM, ZSBio, China), CD3 and CD4, CMV antigen and HSV antigen (Abcam) respectively. (biomedcentral.com)
  • Here, we report a facile antibody conjugation onto gold/silver (Au/Ag) core− shell nanoparticles by stable and uniform embedment of an antibody binding protein, protein G, in silver nanoshells. (datapdf.com)
  • Identification of such tumor-associated cell surface antigen polypeptides, i.e. tumor associated antigens (TAA), allows specific targeting of cancer cells for destruction via antibody-based therapies. (justia.com)
  • The PAO1 transformants containing this 3.6 kb of D3 DNA exhibited identical lipopolysaccharide (LPS) banding patterns to serotype O16 in silver‐stained SDS-PAGE gels and displayed reactivity to an antibody specific for O‐ acetyl groups. (mcmaster.ca)
  • The antibody response to P.aeruginosa, E.coli and K.pneumoniae outer membrane protein (OMP) antigens were investigated by immunoblotting with sequential serum samples during infection in the leukaemic host. (aston.ac.uk)
Antibodies7
  • Increased microimmunofl uorescence assays by using APM virophage antibodies against an Acanthamoeba polyphaga mimivirus and mamavirus APM strain antigens, obtained after amebal virophage indicated seroconversion. (cdc.gov)
  • with purifi ed APM virophage, mamavirus antigens, and A. We describe seroconversion to antibodies against the APM castellanii antigens. (cdc.gov)
  • Such chronic injury is usually mediated by anti-human leukocyte antigen antibodies. (medscape.com)
  • Compared to Au nanoparticles of similar size with randomly adsorbed antibodies, the present immuno-labeled Au/Ag core−shell nanoparticles offered nearly 10-fold higher sensitivities for naked-eye detection of surface bound antigens. (datapdf.com)
  • The present strategy provides a simple but efficient way to conjugate antibodies to nanosilver surfaces, which will greatly facilitate wider use of the superior optical properties of silver nanostructures in biological applications. (datapdf.com)
  • Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS. (lookformedical.com)
  • Anti-GBM disease is the result of antibodies direct against antigens in the glomerular basement membrane. (surgpath4u.com)
Methenamine silver stain2
  • A Gomori methenamine silver stain can then confirm the presence of the Coccidioides organism's characteristic spherules within the tissue. (wikipedia.org)
  • Cryptococcosis is confirmed by microscopy or by staining with PAS, mucicarmine, or methenamine silver stain. (medscape.com)
Biopsy1
  • Investigations include smears, biopsy, staining with periodic acid-Schiff (PAS) or Gomori methenamine silver, culture of the affected tissues, polymerase chain reaction (PCR), serodiagnosis (sometimes), physical examination, and chest radiography. (medscape.com)
Gram5
  • The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid-Schiff) staining, Gram staining, Ziehl-Neelsen stain and Grocott's Methenamine Silver (GMS) Stain. (biomedcentral.com)
  • PAS (Periodic acid-Schiff) staining, Gram staining, Ziehl-Neelsen stain and Grocott's Methenamine Silver (GMS) Staining were performed using commercial kits from Baso (Zhuhai, China) according to the manufacturer's instructions. (biomedcentral.com)
  • Finding a mixture of microorganisms with multiple morphologies and staining characteristics on Gram stain suggests a contaminated specimen or a polymicrobial bacterial infection. (msdmanuals.com)
  • To do a Gram stain, technicians heat-fix specimen material to a slide and stain it by sequential exposure to Gram crystal violet, iodine, decolorizer, and counterstain (typically safranin). (msdmanuals.com)
  • These may include a bacteria culture , viral blood tests, and a Gram stain . (medlineplus.gov)
Tissue6
  • Leptospiral antigens were seen as intact leptospira, thread-like filaments, and granular forms in liver and kidney tissue from three patients. (cdc.gov)
  • A Luxol fast blue-periodic acid-Schiff-stained tissue section (A) was matched to a 4.7-T proton density (PD) image (partly reproduced from Journal of Neurology . (jamanetwork.com)
  • Formic acid antigen retrieval is a low pH tissue pretreatment used in immunohistochemistry. (protocolsonline.com)
  • The use of silver, usually silver nitrate, as a reagent for producing contrast or coloration in tissue specimens. (lookformedical.com)
  • Newcomer Supply Urate Stain, Gomori Methenamine Silver Method is designed to demonstrate urates in tissue sections. (newcomersupply.com)
  • This team member is responsible for preparing tissue for routine microscopic analysis and special histochemical stains. (nsh.org)
Fontana-Masson1
Gels3
  • silver-stained gels (Figure). (cdc.gov)
  • I. Staining of sodium dodecyl sulfate gels. (microbiologyresearch.org)
  • The silver-stained SDS-PAGE gels of proteinase K digested whole cell lysates of strains PAO1, 6750, M7 and PAJ indicated that core LPS was affected in the presence of mitomycin C. In contrast, the rough strain AK1012 showed no observable differences. (aston.ac.uk)
Protein8
  • 1a, Glial fibrillary acidic protein stain (original magnification ×400) of a chronic gliotic area in DAWM. (jamanetwork.com)
  • 2a, Glial fibrillary acidic protein stain (original magnification ×400) of active gliosis with large cell bodies and gemistocytes (inset, original magnification ×400) in normal-appearing WM (NAWM). (jamanetwork.com)
  • No relation was found between MTR and transmittance (TM) of sections stained for proteolipid protein (Tm [PLP] ). (jamanetwork.com)
  • The receptor-binding cancer antigen expressed on SiSo cell (RCAS1) is a cancer-specific protein that induces the apoptosis of peripheral lymphocytes and confers tumor immune evasion. (bvsalud.org)
  • However, results of Western blot experiments demonstrated that the antigens reactive with 2C1 were not actually the 39K protein but the O-side chains of the LPS. (aai.org)
  • Uniform silver nanoshells were successfully formed on these protein G-AuNPs, while stably embedding protein G-linked peptide layers. (datapdf.com)
  • Analysis of toxoplasmosis in pregnant girls utilizing dot-immunogold-silver staining with recombinant Toxoplasma gondii peroxiredoxin protein Background: Toxoplasma gondii an infection endangers human well being and impacts animal husbandry. (chem2pharm.com)
  • The targeted antigen is the non-collagenous domain (NC1) of the alpha3-chain of collagen type IV, which is an important protein in the maintenance of the GBM architecture (Kumar). (surgpath4u.com)
Gomori1
  • Staining with PAS or Gomori methenamine silver shows regular, narrow (4 µm wide), branching, and septate hyphae of Aspergillus species. (medscape.com)
Detection2
  • The Gallyas Silver stain is used in neuropathology for the detection of neurofibrillary tangles, associated with neurodegenerative disorders like Alzheimer's disease. (protocolsonline.com)
  • The Bielschowsky Silver Stain is a classical histological silver stain used for the detection of pathology associated with Alzheimer's disease. (protocolsonline.com)
Smears1
  • Smears may also be stained with India ink or nigrosin. (medscape.com)
Urine2
Impregnation1
  • We have devised an approach to readily label microscopic sections for multiple antigens (neurotransmitters, enzymes, peptides, etc.) as well as for the demonstration of degenerating structures by silver impregnation. (erowid.org)
Immunohistochemistry4
Yeast1
  • Image 4: GMS staining better defines individual yeast making recognition easier at scanning magnifications. (cap.org)
Polyclonal1
  • These findings were confirmed by immunohistochemical staining using rabbit polyclonal reference antiserum reactive with 16 different leptospiral strains. (cdc.gov)
Fungi1
  • Methenamine silver or PAS staining is best to show the fungi, which are difficult to culture from a swab sample. (medscape.com)
Retrieval1
  • Hydrochloric acid antigen retrieval is a technique used to improve staining on paraffin embedded tissues. (protocolsonline.com)
Reactive1
  • Hence, the most likely explanation for our observation is that the 37 degrees C incubation condition induced a partial smooth to rough transition of the Yersinia LPS with a decrease in the amount of 2C1-reactive antigen. (aai.org)
Eosin1
  • Intestinal tissues were fixed with 4% neutral formaldehyde for 6-10 h, routinely dehydrated and embedded with paraffin, 4 μM sections were serially cut on APES (3-Aminopropyltriethoxysilane) coated slides and stained with hematoxylin and eosin (H&E) and observed on a light microscope. (biomedcentral.com)
Periodic1
  • T1 and T2 relaxation times were related to myelin density, measured as light transmittance on sections stained for Luxol fast blue-periodic acid-Schiff (Tm (LFB-PAS) ). (jamanetwork.com)
Capsule1
  • Rupture of the glomerular basement membrane and Bowman's capsule may be seen, especially if highlighted by silver stain. (surgpath4u.com)
Methods2
  • if these pathogens are suspected, different stains or other identification methods are required. (msdmanuals.com)
  • Luna, Lee G. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology . (newcomersupply.com)
Paraffin1
  • Plastic ( Part 5500 ), plastic-tipped, or paraffin coated metal forceps must be used with any silver solution to prevent precipitation of silver salts. (newcomersupply.com)
Diagnostic1
  • Stool samples were obtained and assayed for rotavirus antigens by enzyme linked immunosorbent assay technique using standard diagnostic BIOLINE Rotavirus kit. (biomedcentral.com)
Slides2
  • Incubate slides in preheated Methenamine Silver Working Solution in a 60°C water bath for 30 minutes. (newcomersupply.com)
  • Glass Bowls With Steel Slide Holder - For Staining - Capacity For Atleast 10 Slides. (haryanatenders.net)
Suggests1
Procedure3
  • Improved silver staining procedure for fast staining in PhastSystem development unit. (microbiologyresearch.org)
  • Reagents for this procedure are sold as individual stain solutions and are available for purchase under separate part numbers with storage requirements and expiration date designated per bottle. (newcomersupply.com)
  • All Newcomer Supply stain procedures are designed to be used with Coplin jars filled to 40 ml following the provided staining procedure. (newcomersupply.com)
Cell3
  • and orange dots, areas of antigen-presenting cell activation. (jamanetwork.com)
  • Antigen-presenting cell (APC) activation was more pronounced in DAWM. (jamanetwork.com)
  • The rough cells had long, thin fibrillar structures outside the cell envelope when they were stained negatively for electron microscopy. (microbiologyresearch.org)
Sodium1
  • The LPS preparations were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. (aai.org)
Pretreatment1
  • After pretreatment, hybridization and amplification, sections were finally stained with NBT and BCIP (Roche) in developing solution at 37 °C and counterstained with nuclear fast red (Vector Labs, USA). (biomedcentral.com)
Reagents1
  • The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. (hindawi.com)
Assay1
  • Silver enhancement of the bound nanoparticles may be used to increase the sensitivity of the assay. (hindawi.com)
Counterstain1
  • Counterstain in Light Green SF Yellowish Stain 0.2%, Aqueous ( Part 12202 ) for 1-2 minutes. (newcomersupply.com)
Immune3
  • We identified a prokaryotic and eukaryotic dual-expression vector system, pJHL270, that can provide simultaneous MHC class I and II stimulation of the host immune system, and we designed vaccine antigens by selecting the consensus HA1 sequence and M2e antigens from H9N2 virus circulating in South Korea from 2000 to 2021. (bvsalud.org)
  • Chickens immunized with JOL2922 yielded high antigen-specific humoral and mucosal immune responses. (bvsalud.org)
  • in contrast, a granular staining pattern is seen in circulating immune complex deposition. (surgpath4u.com)
Laboratory1
  • The histotech is responsible for laboratory equipment routine maintenance and documentation, embedding and microtomy, slide staining and coverslipping, supply inventory and ordering, troubleshooting laboratory issues including processor issues, stain optimization, microtome adjustments and coverslipper maintenance. (nsh.org)
Test1
  • In A Pack Size Of 2.5 Litre , Methyl Green, In A Pack Size Of 5Gm, 25Gm , Methylene Blue 25Gm , Methylene Bluesolution 125Ml , Multistix ( Minimum 10 Parameters ) , In A Pack Size Of 100 Test , Myelo Peroxidase Stain, In A Pack Size Of 25 Gm. (haryanatenders.net)
Technique1
  • H&E stains are the most common staining technique used for histologic studies. (protocolsonline.com)
Adequate1
  • Remove control slide, rinse in warm distilled water, check microscopically for adequate silver development. (newcomersupply.com)
Specific1
  • It has been suggested that the O-side chains of the lipopolysaccharide (LPS) of serotype 0:3 strains of Yersinia enterocolitica vary quantitatively and qualitatively depending on whether they are cultured at 37 degrees C or 25 degrees C. It is uncertain whether this affects the expression of the serotype-specific antigens that are probably carried on the LPS. (aai.org)
Density1
  • Single oral doses of 40 or 80 mg/kg MDMA doubled the density of silver-impregnated (degenerating) fiber terminals in the caudate nucleus compared to controls, when rats were sacrificed 18 hours after treatment. (erowid.org)
Samples1
  • Moreover, we report the development of a silver enhancement technology that operates even in the presence of high chloride concentrations as may be encountered in biologic samples. (hindawi.com)
Show1
Solution5
Expression1
  • The Ptrc and CMV promoters conferred antigen expression in prokaryotic and eukaryotic cells to induce balanced Th-1/Th-2 immunity. (bvsalud.org)
Positive1
  • Image 3: PAS staining showing positive staining between glandular clefts. (cap.org)
Production1
  • OMV produced by bacteria, genetically modified to increase their spontaneous release, simplifying their production, are also known as Generalized Modules for Membrane Antigens (GMMA). (mdpi.com)