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  • recombinant
  • Microalgae could be poised to become the next candidate in recombinant subunit vaccine production, as they present several advantages over terrestrial crop plant-based platforms including scalable and contained growth, rapid transformation, easily obtained stable cell lines, and consistent transgene expression levels. (frontiersin.org)
  • In this perspective, edible plants have also been thought as a tool to deliver by the oral route recombinant compounds of medical significance for new therapeutic strategies. (springer.com)
  • Milestone for plant-based recombinant protein production for human health use was the approval in 2012 by the US Food and Drug Administration of plant-made taliglucerase alfa, a therapeutic enzyme for the treatment of Gaucher's disease, synthesized in carrot suspension cultures by Protalix BioTherapeutics. (springer.com)
  • Capodicasa C, Catellani M, Moscetti I, Bromuro C, Chiani P, Torosantucci A, Benvenuto E (2017) Comparative analysis of plant-produced, recombinant dimeric IgA against cell wall β-glucan of pathogenic fungi. (springer.com)
  • Expression
  • The transgenic nature of the plants and expression of the antigen was confirmed by PCR, Southern hybridization and reverse transcription (RT)-PCR. (springer.com)
  • The expression levels of the antigen in the plants grown under in vitro conditions as well as the green house hardened plants were estimated by ELISA for all the four constructs. (springer.com)
  • Maximum expression level of 38 ng/g F.W. of leaves was noted in plants transformed with pEFEHBS grown under in vitro conditions, whereas pHER transformed plants grown in the green house showed the maximum expression level of 19.92 ng/g F.W. of leaves. (springer.com)
  • We have previously defined the promoter elements, sites IIa and IIb, in the rice proliferating cell nuclear antigen (PCNA) gene that are essential for meristematic tissue-specific expression. (plantcell.org)
  • In this work, a transient expression system for the production of human TG2 in Nicotiana benthamiana leaves was optimized and reactivity of plant-produced TG2 in CD screening test was evaluated. (nih.gov)
  • Expression of tuberculosis antigen ESAT-6 in Nicotiana tabacum using a potato virus X-based vector," Tuberculosis , vol. 86, no. 3-4, pp. 263-267, 2006. (hindawi.com)
  • Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in transgenic plants by elastin-like peptide fusion strategy," Journal of Biomedicine and Biotechnology , vol. 2010, Article ID 274346, 14 pages, 2010. (hindawi.com)
  • Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M (2014) A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants. (springer.com)
  • Since each plant cell contains up to 10,000 copies of identical plastid genome, plastid engineering should result in very high levels of transgene expression. (openthesis.org)
  • gene
  • The authors thank Dr. G.D. May, Boyce Thompson Institute for Plant research Inc., Ithaca, NY, USA, for providing the EFE gene promoter of banana. (springer.com)
  • Hood EE, Gelvin SB, Melchers LS, Hoekama A (1993) New Agrobacterium helper plasmid for gene transfer to plant cells. (springer.com)
  • PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene. (plantcell.org)
  • epitopes
  • Plant purified ER-TG2 and vac-TG2 were recognized by three anti-TG2 monoclonal antibodies that bind different epitopes proving that plant-produced antigen has immunochemical characteristics similar to those of human TG2. (nih.gov)
  • To test their usefulness as antigen their recognition by the mAbs 2G3, 5G7, or 4E1, which recognize different TG2 epitopes, was analyzed by immunoblot. (nih.gov)
  • Both vac-TG2 and ER-TG2 were positively recognized by these antibodies as is shown Figure 4A, confirming that although in humans TG2 is a localized in the cytosol, the introduction into the plant secretory pathway do not affect the structure of the epitopes recognized by these mAbs. (nih.gov)
  • systemic
  • When administered by the oral route in a four-weekly dose immunization scheme, the plant-derived C4V3 has elicited both systemic and mucosal antibody responses in BALB/c mice, as well as CD4+ T cell proliferation responses. (deepdyve.com)
  • immunogenic
  • This study provided the proof of principle for rapidly producing immunogenic vaccine candidates against WNV in plants with low cost and scalability. (hindawi.com)
  • Thus, we show that transgenic plants can be readily used to express and produce Rift Valley Fever virusproteins, and that the plants are immunogenic when given orally to mice. (diva-portal.org)
  • immunology
  • In: Hammond J, Mc Garvey P, Yusibov V (eds) Current topics in microbiology and immunology, plant biotechnology: new products and applications, vol 240. (springer.com)
  • Immunological characterization of novel secreted antigens of Mycobacterium tuberculosis ," Scandinavian Journal of Immunology , vol. 61, no. 2, pp. 139-146, 2005. (hindawi.com)
  • ELISA
  • In order to test the performance of the plant purified ER-TG2 and vac-TG2 version in CD screening test an ELISA was performed using a pool of 12 sera of CD patient and control healthy donors (Figure 4B). (nih.gov)
  • biotechnology
  • Dickman MB (2004) Can model plants help banana improvement through biotechnology? (springer.com)
  • Use of transgenic carrot plants producing human interleukin-18 for modulation of mouse immune response," in New Research on Biotechnology in Biology and Medicine , A. M. Egorov and G. Zaikov, Eds. (hindawi.com)
  • Since October 2010, she has been appointed as the UNMC Director of Biotechnology Research Centre which facilitates different areas of molecular biotechnology and plant tissue culture researches. (nottingham.ac.uk)
  • I have also been contributing teaching in "Plant Biotechnology", "Introductory Plant Pathology", and "Molecular Plant Pathology" under the BSc Plant Biotechnology and Biotechnology programmes and "Plant Genetic Manipulation Practical Techniques" module under the MSc Crop Biotechnology programme. (nottingham.ac.uk)
  • glycoproteins
  • 1979. Cell surfaces in plant-microorganism interactions II, Evidence for the accumulation of hydroxyproline-rich glycoproteins in the cell wall of diseased plants as a defense mechanism. (springer.com)
  • Radioimmunoassay inhibition tests show that the antiserum to this glycoprotein cross-reacts with a number of other plant glycoproteins. (biochemj.org)
  • tobacco plants
  • Barton KA, Binns AN, Matzke AJ, Chilton MD (1983) Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny. (springer.com)
  • cellular
  • The basic principle behind the practice of immunization is that the body's own immune system is stimulated to produce a humoral or cellular response against a specific disease-causing organism by exposing the system to an antigen of the causative agent without actually bringing on the full symptoms of the disease. (google.ca)
  • Results
  • These results confirmed the usefulness of plant-produced TG2 to develop screening assays. (nih.gov)
  • Several
  • These efforts have been successful as evidenced by the fact that several antibodies and vaccine antigens produced by magnifection are currently in clinical development. (springer.com)
  • fungal
  • 1984. Induction of resistance-related mRNAs by UV light or fungal elicitor in cultured plant cells. (springer.com)
  • Plants when exposed to oligosaccharides of fungal origin, defend themselves by accumulating antibiotics. (springer.com)
  • compounds
  • There is a growing interest in discovering plant-based anti-inflammatory compounds as potential alternatives to conventional drugs. (peerj.com)
  • Plants and their rich variety of natural compounds are used to maintain and to improve health since the earliest stages of civilization. (springer.com)
  • major
  • Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6 · CFP-10 complex. (hindawi.com)
  • cell
  • Thank you for your interest in spreading the word on Plant Cell. (plantcell.org)
  • Message Body (Your Name) thought you would like to see the Plant Cell web site. (plantcell.org)
  • Exocytosis and cell polarity in plants - exocyst and recycling domains. (cas.cz)
  • similar
  • The loss of self-tolerance is believed to be due to many hereditary and environmental factors and occurs when autoantigens are damaged, when they link with a foreign antigen, when the structure of a autoantigen is very similar to that of a foreign antigen (molecular mimicry), or when autoreactive T cells are not adequately controlled or are activated by nonspecific antigens. (livehopelupus.org)