• Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. (wikipedia.org)
  • AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies. (stratech.co.uk)
  • Detection methods such as fluorescence analysis and surface plasmon resonance are summarized. (liverx-receptor.com)
  • The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. (patsnap.com)
  • Fluorescence polarization immunoassays employ a fluorophore bound antigen that when bound to the antibody of interest, will increase fluorescence polarization. (wikipedia.org)
  • Since Perrin's contribution, the technique has grown from determining binding isotherms under heavily controlled parameters, to the study of antigen-antibody, small molecule-protein, and hormone-receptor binding interactions. (wikipedia.org)
  • FPIA quantifies the change in fluorescence polarization of reaction mixtures of fluorescent-labelled tracer, sample antigen, and defined antibody. (wikipedia.org)
  • Free tracer in solution has a lower fluorescence polarization than antibody-bound tracer with slower Brownian motion. (wikipedia.org)
  • The tracer and the specific antigen will compete to bind to the antibody and if the antigen is low in concentration, more tracer will be bound to the antibody resulting in a higher fluorescence polarization and vice versa. (wikipedia.org)
  • Therefore, by synthesizing the N gene sequence of SARS-CoV-2, constructing the pET-28a (+)-N recombinant plasmid, we expressed the N protein in Escherichia coli and obtained 15 monoclonal antibody (mAbs) against SARS-CoV-2-N protein by the hybridomas and ascites, then an immunochromatographic test strip method detecting N antigen was established. (bvsalud.org)
  • A combination of HS201-PDT with anti-PD-L1 antibody demonstrated the greatest antigen-specific immune response, tumor growth suppression, prolonged mouse survival time and abscopal effect. (bmj.com)
  • Iron nanoparticles are conjugated with two types of antibodies (one against antigens on therapeutic cells and the other directed at injured cells) to produce magnetic bifunctional cell engager (MagBICE). (regenerativemedicine.net)
  • FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays. (stratech.co.uk)
  • When a fluorescent-labelled molecule (tracer) binds to another molecule the rotational motion will change, resulting in an altered intensity of plane-polarized light, which results in altered fluorescence polarization. (wikipedia.org)
  • DC are professional antigen-presenting cells that are essential for initiating adaptive immune responses. (biomedcentral.com)
  • These professional antigen-presenting cells are able to discriminate unmodified self from non-self thanks to pattern recognition receptors such as the Toll like Receptors (TLR) and Scavenger Receptors (SR). SR were originally identified by their ability to bind and internalize modified lipoproteins and microorganisms but also particles and TLR agonists. (biomedcentral.com)
  • The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. (patsnap.com)
  • The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening). (patsnap.com)
  • We examined factors associated with PCP beliefs about screening efficacy and uncertainty and whether beliefs were associated with prostate specific-antigen (PSA) test use. (cdc.gov)
  • The fluorescence polarization value for the tracer containing solution is compared to the baseline and magnitude of difference is proportional to quantity of target analyte in sample. (wikipedia.org)
  • It would be highly desirable to perform such measurements rapidly and with reduced sample volumes to support point-of-care testing. (hes-so.ch)
  • Second, this higher photo-sensitivity results in less measurement error, which minimizes spreading. (beckman.de)
  • These mAbs can be used for the early and rapid diagnosis of SARS-CoV-2 infection through serological antigen. (bvsalud.org)
  • Measurement of free radicals in the culture medium of developing embryos has been reported as an index for embryo viability. (biomedcentral.com)
  • 7. Measurement of alpha-fetoprotein, carcinoembryonic antigen and prostate-specific antigen in serum and heparinised plasma by enzyme immunoassay on the fully automated serono SR1 analyzer. (nih.gov)
  • We examined factors associated with PCP beliefs about screening efficacy and uncertainty and whether beliefs were associated with prostate specific-antigen (PSA) test use. (cdc.gov)
  • 4. Effects of serum indices interference on hormonal results from the Abbott Architect i2000 immunoassay analyser. (nih.gov)
  • Competitive ratiometric fluorescent lateral flow immunoassay based on dual emission signal for sensitive detection of chlorothalonil. (nih.gov)
  • Measurement of the polarization of fluorescent light from solutions or microscopic specimens. (nih.gov)
  • When a fluorescent-labelled molecule (tracer) binds to another molecule the rotational motion will change, resulting in an altered intensity of plane-polarized light, which results in altered fluorescence polarization. (wikipedia.org)
  • Observing that polarization increased with viscosity of the solvent and the size of the dye molecule, but decreased with an increase in temperature, he deduced that polarization increased with a decrease in mobility of the emitting species. (wikipedia.org)
  • 11. Uncertainty of measurement for 14 immunoassay analytes: application to laboratory result interpretation. (nih.gov)
  • The Spark Cyto microplate reader allows various detection technologies, including highly advanced technologies like TR-FRET, Fluorescence Polarization, ALPHA® technology and image-based cytometry, to be combined for the investigation of cellular signaling pathways. (tecan.com)
  • Easy to setup, use and maintain, the new EnVision Nexus platform works across major detection technologies, including fluorescence intensity, fluorescence polarization, absorbance, luminescence and time-resolved fluorescence (TRF). (selectscience.net)
  • But in those cases the cell is fixed, and if the cell is live, the fluorescence of the small molecules lasts at most for 20 minutes," said Mr. Chaudhari.Optical scattering is one of the other methods used for studying biological events. (medical-hospital-equipment.com)
  • 20. Validating a custom multiplex ELISA against individual commercial immunoassays using clinical samples. (nih.gov)
  • 17. Performance of Opus immunoassays for thyroxine and beta-human chorionic gonadotrophin in serum. (nih.gov)
  • ETS exposure will also be assessed for examinees through the measurement of serum cotinine, a metabolite of nicotine. (cdc.gov)
  • Thus, the formed proximity hybridization enabled the occurrence of fluorescence RET (FL-RET, as the primary RET) between Ag NCs (donor) and Pt NPs (acceptor), quenching FL intensity of Ag NCs (FL off). (bvsalud.org)
  • If it is necessary to detect all antibodies that bind to antigen, conjugates prepared with antibodies specific for Ig κ and λ light chains should be used. (amrita.edu)
  • Although individual proteins may require specific conditions or pretreatment for optimal binding, the most common method for coating plates involves adding a 2-10 μg/ml solution of protein dissolved in an alkaline buffer such as phosphate-buffered saline (pH 7.4) or carbonate-bicarbonate buffer (pH 9.4).The buffer contains no other proteins that might compete with the target antigen for attachment to the microtiter plate. (amrita.edu)
  • Binding measurements can be performed using purified membrane targets or proteins in complex environment such as cell membrane. (calixar.com)
  • Unlike conventional approaches that would alter the native structure of membrane proteins, CALIXAR's approach allows the conservation of the functional and structural integrities of the targets/ antigens. (calixar.com)
  • Dorland:Dorlands_Illustrated_Medical_Dictionary--31st_Ed, ISBN:978-1416049982, PMID:22654893] [Term] id: MMO:0000001 name: electrical potential recording method def: "A method using the work per unit charge necessary to move a charge body in an electric field from a reference point to make or calculate the measurement of interest. (mcw.edu)
  • ex vivo method created_by: mshimoyama creation_date: 2010-06-29T08:03:34Z [Term] id: MMO:0000005 name: post excision weight measurement def: "Any method for measuring the weight of an organ, tissue or other body part after it has been removed from the body. (mcw.edu)
  • in vivo method [Term] id: MMO:0000011 name: vascular indwelling catheter method def: "A thin tube communicating to the body surface, inserted into the vascular system and positioned to permit measurements of blood flow, pressure or blood sampling over a period of time. (mcw.edu)
  • A specific quantity of antigen conjugated with fluorophore is added to the solution. (wikipedia.org)
  • The antigen is usually diluted in blocking buffer to prevent non specific attachment of protein in the antiserum on the solid phase. (amrita.edu)
  • The Spark Cyto live cell microplate reader provides predefined applications to easily assess transfection efficiency and reporter activity, using standard fluorometric and luminometric detection capabilities, as well as fluorescence-based cell imaging. (tecan.com)
  • The fluorescence polarization value for the tracer containing solution is compared to the baseline and magnitude of difference is proportional to quantity of target analyte in sample. (wikipedia.org)
  • Excess antigen or unbound antigen are removed by washing step and is followed by addition of blocking solution. (amrita.edu)
  • 5. LIAISON hCG--an automated chemiluminescent immunoassay for the determination of human chorionic gonadotropin (hCG). (nih.gov)
  • Cytotoxic T lymphocyte-associated antigen-4-Ig (CTLA-4-Ig) suppresses Staphylococcus aureus-induced CD80, CD86, and pro-inflammatory cytokine expression in human B cells. (medscape.com)