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  • monoclonal antibody
  • This defect in DC migration after hapten sensitization was associated with defective CHS responses and decreased cutaneous tumor necrosis factor (TNF)-α production and was corrected by injecting recombinant TNF-α or an agonistic anti-CD40 monoclonal antibody. (rupress.org)
  • vitro
  • In vitro generated DCs from CD34 progenitor cells or monocytes rapidly synthesize the message in response to CD40 ligation and a mixed lymphocyte reaction (B) results in decysin expression together with CD83. (nih.gov)
  • vivo
  • In contrast, the therapeutic potential of autologous antigen-specific T cells has yet to be established since it has been technically difficult to generate sufficient numbers of these T cells, ex vivo. (pubmedcentralcanada.ca)
  • We, therefore, contend that CD40-activated B cells are an alternative source of highly efficient APCs with which to generate antigen-specific T cells ex vivo for autologous adoptive immunotherapy. (pubmedcentralcanada.ca)
  • Similarly, in another ex vivo isolate, blood CD11c+ DCs do not contain detectable decysin mRNA, but maturation in culture (23) and more importantly CD40 activation result in decysin induction. (nih.gov)
  • helper
  • Helper T cells can produce CD40L de novo upon antigen-specific interactions, but they also have an intracellular secretory compartment containing a small amount of preformed CD40L that is brought to the T cell surface rapidly upon antigen recognition. (ohsu.edu)
  • I found that despite distinct immunological synapse structures, preformed CD40L is delivered in an antigen-specific manner by both Th1 and Th2 helper T cells. (ohsu.edu)
  • Mice
  • In mice transgenic for an autoantibody, self-reactive B cells have been shown to be eliminated upon interaction with membrane-bound self-antigens in the periphery as well as in the bone marrow, suggesting that both immature and mature B cells are eliminated by multimerization of surface immunoglobulins (sIg). (nih.gov)
  • monocytes
  • 2 When exiting the blood stream, monocytes can give rise to inflammatory macrophages or to antigen presenting DCs. (bloodjournal.org)
  • Monocytes and macrophages play a key role in a number of immune functions including inflammation, antigen presentation and the clearance of microorganisms and free hemoglobin. (dartmouth.edu)
  • affinity
  • Transfer is much more efficient to antigen-bearing than to bystander B cells, a finding that may explain the phenomenon of affinity maturation of the antibody response, a process that is required for generation of the high affinity antibodies that protect us from infectious agents and their toxins. (ohsu.edu)
  • In the germinal center reaction, rare somatic mutant B cells with higher affinity for antigen are selected for survival and eventually exported as long-lived memory B cells and antibody secreting cells. (ohsu.edu)
  • Glycan structure does not influence binding affinity to CD40. (abcam.com)
  • Diseases
  • Diseases associated with CD40 include Immunodeficiency With Hyper-Igm, Type 3 and Acute Myocarditis . (genecards.org)
  • activation
  • Indeed, 28 cycle PCR coupled to reverse transcribed RNA (RT-PCR) on freshly isolated GCDCs failed to detect decysin (Fig. 4 A). Its expression is induced by spontaneous maturation in culture (4) and increases in response to CD40 activation. (nih.gov)
  • Nuclear factor kappaB (NF-kappaB)-inducing kinase (NIK) has been implicated as a central transducing kinase in CD40-dependent activation. (nih.gov)
  • Such data provide compelling evidence that different intermediary kinases are used by different cellular lineages to trigger NF-kappaB activation via CD40. (nih.gov)
  • human
  • CD40-activated human B cells: an alternative source of highly efficient antigen presenting cells to generate autologous antigen-specific T cells for adoptive immunotherapy. (pubmedcentralcanada.ca)
  • Human CASMCs (coronary artery smooth muscle cells) in culture exposed to IL (interleukin)-1beta or TNF-alpha (tumour necrosis factor-alpha) had increased superoxide generation and enhanced CD40 expression, detected by EPR (electron paramagnetic resonance) and immunoblotting respectively. (mendeley.com)
  • Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD40(GTX101447) antibody at 1:250 dilution. (bio-connect.nl)
  • increases
  • In the present study, we tested the hypothesis that AngII increases CD40/CD40L activity in vascular cells and that ROS (reactive oxygen species) are part of the signalling cascade that controls CD40/CD40L expression. (mendeley.com)
  • naive
  • In addition, PGE2 inhibited IgE production by naive surface IgD+ B cells cultured in the CD40 system, suggesting that PGE2 may interact with mechanisms involved in IgE switching. (jimmunol.org)
  • bind
  • The oligonucleotides are targeted to bind to primary transcripts CD40, CD80, CD86 and their combinations. (google.com)
  • dependent
  • It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. (nih.gov)
  • expression
  • Increased CD40 expression led to enhanced activity of the pathway, as AngII-treated cells stimulated with recombinant CD40L released higher amounts of IL-8 and had increased COX-2 (cyclo-oxygenase-2) expression. (mendeley.com)
  • CD8(+) T cells responding to alveolar self-antigen lack CD25 expression and fail to precipitate autoimmunity. (semanticscholar.org)