• The section was costained with anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD31 Antibody (clone MEC13.3) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-Tubulin β 3 (TUBB3) Antibody (Clone TUJ1) Alexa Fluor® 647 at 5 µg/mL (magenta). (biolegend.com)
  • Confocal image of C57BL/6 mouse lung sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD11c (magenta) in Cycle 1, CD206 (blue) in Cycle 1, and CD31 (green) in Cycle 4. (biolegend.com)
  • The following product was used in this experiment: CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-0319-82, RRID AB_468934. (thermofisher.com)
  • Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. (thermofisher.com)
  • Immunofluorescent double staining was used to detect YAP cellular localization with CD31 (an endothelial cell marker) antibody. (molvis.org)
  • Various lysates were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens). (ptglab.com)
  • Left Panel) or BD Horizon™ BV510 Rat Anti-Mouse CD31 antibody (Cat. (bdbiosciences.com)
  • The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). (bdbiosciences.com)
  • Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 ( MCA2388 ) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody ( STAR72 ). (bio-rad-antibodies.com)
  • Dear Histonetters, I do not think there is a good CD31 (PECAM-1) antibody that will work on FFPE mouse tissues. (utsouthwestern.edu)
  • The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51/CD61). (biolegend.com)
  • CD31 on endothelial cells binds to the CD38 receptor on natural killer cells for those cells to attach to the endothelium. (wikipedia.org)
  • Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. (elifesciences.org)
  • Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)
  • CD31 (PECAM-1) is an adhesion receptor with signaling function that is implicated in vascular wound healing, angiogenesis and transendothelial migration of leukocyte inflammatory responses. (bioinf.org)
  • There is an unmet need to develop novel therapies for refractory/relapsed MM. In the past few years, chimeric antigen receptor (CAR)-modified T cell therapy for MM has shown promising efficacy in preclinical and clinical studies. (biomedcentral.com)
  • Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
  • Immunohistochemical analysis that demonstrates the endothelial nature of the neoplasm is frequently positive for specific vascular markers (CD31, CD34, factor VIII), and keratin 1,2,5,6 . (bvsalud.org)
  • Around the contrary, no cellsexpressed markers of hematopoietic lineage (CD14 and CD45), hematopoietic progenitor (CD34) or endothelial cells (CD31, vWF). (amparinhibitor.com)
  • CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. (biolegend.com)
  • Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a protein that in humans is encoded by the PECAM1 gene found on chromosome17q23.3. (wikipedia.org)
  • CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. (thermofisher.com)
  • Mouse monoclonal to CD31.COB31 monoclonal reacts with human CD31, a 130-140kD glycoprotein, which is also known as platelet endothelial cell adhesion molecule-1 (PECAM-1). (bioinf.org)
  • CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. (thermofisher.com)
  • The CD31 molecule has also been found in metastatic colon carcinoma. (bioinf.org)
  • Several antigens have been used as targets for CAR-T cell therapy against MM, including B cell maturation antigen (BCMA), CD19, CD138, signaling lymphocytic activation molecule 7 (SLAM7), and immunoglobulin light chains. (biomedcentral.com)
  • The isolated cells also constituting expressed of HLA-G antigen, a well-known tolerogenic molecule involved within the immuomodulatory activity of mesenchymal stromal/stem cells [17] (Figure 2A). (amparinhibitor.com)
  • T cell-based immunotherapy, such as immune checkpoint blockade or adoptive T cell transfer, is limited by the ability of T cells to detect major histocompatibility complex (MHC)-presented antigen by tumor cells. (elifesciences.org)
  • Two main advantages of BCMA as an antigen for CAR-T therapy are the potential reduction of on-target/off-tumor toxicity and the lack of antigen-dependent reduction in CAR-T cell expansion [ 16 ]. (biomedcentral.com)
  • Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques. (wikidoc.org)
  • hC-MSCs were elongated and homogeneously spindle-shaped in morphology with thin cytoplasmic projections (Figure 1H).Human cadaver mesenchymal stromal/stem cell phenotypic and molecular PRMT8 Formulation characterizationAt the third replaying, flow cytometry analysis showed that hC-MSCs expressed recognized markers of hMSCs (CD44, CD73, CD90 and CD105), pericyte antigens (CD146, PDGF-r and NG2) and stemness markers (Stro-1, Oct-4 and Notch-1). (amparinhibitor.com)
  • With regards to pericyte antigens, 99.four of CD44+/CD90+ coexpressed PDGF-r and 74 of CD44+/CD90+ stained with CD146 (Figure 2B). (amparinhibitor.com)
  • Proliferating cell nuclear antigen (PCNA) expression in CNV mice without or with YAP siRNA intravitreal injection and the colocalization of PCNA and CD31 were measured with western blotting and immunofluorescent double staining, respectively. (molvis.org)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • Murine studies revealed that Nell-1 haploinsufficiency results in marked reductions in the numbers of Sca-1+CD45-CD31- bone marrow MPCs associated with low bone mass. (nih.gov)
  • Conversely, rhNELL-1 systemic administration in mice showed a marked anabolic effect accompanied by increased numbers of Sca-1+CD45-CD31- bone marrow MPCs. (nih.gov)
  • Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction. (thermofisher.com)
  • CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. (bdbiosciences.com)
  • Carcinoembryonic antigen (CEA) is a glycoprotein involved in cell adhesion . (wikidoc.org)
  • Results: No difference in antigen expression between normal and malignant endothelial cells (ECs) was found for CD54, CD109, CD137, CD141, CD144 and CXCR7. (researchgate.net)
  • Hammarstrom S. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. (wikidoc.org)
  • Methods: Identification of tumour-associated endothelial markers (TEM) by comparing antigen expression on normal vs t-CEC and assess the presence of t-CEC in peripheral blood of cancer patients by incorporating TEM in our novel flow cytometry-based CEC detection assay. (researchgate.net)
  • The CD31 antigen is expressed on platelets and endothelial cells at high levels, as well as on T-lymphocyte subsets, monocytes, and granulocytes. (bioinf.org)
  • Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). (ptglab.com)
  • There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose protected cryo embedded mouse tissues with antigen retrieval. (utsouthwestern.edu)
  • C57BL/6 mouse splenocytes were stained with CD31 (clone MEC13.3) Alexa Fluor® 594 (filled histogram) or rat IgG2a, κ Alexa Fluor® 594 isotype control (open histogram). (biolegend.com)
  • Then the section was stained with 2.5 µg/ml of CD31 (clone MEC13.3) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). (biolegend.com)
  • Then the tissue section was stained with 5 µg/mL of anti-mouse CD31 (clone MEC13.3) Alexa Fluor® 594 (red) and 2.5 µg/mL anti-mouse CD326 (clone G8.8) Alexa Fluor® 488 (blue) and 2.5 µg/mL anti-mouse Tubulin Beta 3 (clone AA10) Alexa Fluor® 647 (green) overnight at 4°C. The image was captured by 10X objective. (biolegend.com)
  • Malignant endothelial cells also commonly retain the antigen, so that CD31 immunohistochemistry can also be used to demonstrate both angiomas and angiosarcomas. (wikipedia.org)
  • Natural killer (NK) cell-based immunotherapy may overcome genetic mechanisms of resistance to T cell-based immunotherapy through antigen- and MHC-independent recognition of malignant cells. (elifesciences.org)
  • In bone tissue marrow ST 2825 produced murine macrophage lines, interferon gamma (IFN-) elevated bradyzoite antigen appearance and this were linked to nitric oxide (NO) creation (Bohne et al. (thetechnoant.info)
  • The same CD31 Rat mono from BD will also work on Zinc Fixative from BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but no aldehyde) fixed paraffin embedded mouse tissues. (utsouthwestern.edu)
  • N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. (thermofisher.com)
  • Prolonged rhNELL-1 protein release was achieved using an apatite-coated β-tricalcium phosphate carrier, resulting in a local influx of stem cell antigen-1-positive (Sca-1+) mesenchymal progenitor cells (MPCs), and complete osseous fusion across all samples (100% spinal fusion rate). (nih.gov)
  • Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis. (biolegend.com)
  • Two-color flow cytometric analysis of CD31 expression on BALC/c mouse bone marrow cells. (bdbiosciences.com)
  • Figure A. RPE conjugated rat anti mouse CD45R ( MCA1258PE ) and FITC conjugated rat IgG2a isotype control ( MCA6005F ) Figure B. RPE conjugated rat anti mouse CD45R ( MCA1258PE ) and FITC conjugated rat anti mouse CD31 ( MCA2388F ). (bio-rad-antibodies.com)
  • Figure B. Alexa647® conjugated rat anti mouse CD22 ( MCA2187A647 ) and Alexa488® conjugated rat anti mouse CD31 ( MCA2388A488 ). (bio-rad-antibodies.com)
  • Figure A. RPE conjugated rat anti mouse CD45R ( MCA1258PE ) and FITC conjugated rat IgG2a isotype control ( MCA6005PB ) Figure B. RPE conjugated rat anti mouse CD45R ( MCA1258PE ) and Pacific Blue conjugated rat anti mouse CD31 ( MCA2388PB ). (bio-rad-antibodies.com)
  • Two-color flow cytometric dot plots show the correlated expression patterns of CD31 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. (bdbiosciences.com)
  • In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial cells in histological tissue sections. (wikipedia.org)
  • CD31/PECAM-1 impacts engraftment, growth and spread of mantle cell lymphoma cells and positively correlates with extramedullary involvement. (invivogen.com)
  • CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. (thermofisher.com)
  • Failed T cell-based immunotherapies in the presence of genomic alterations in antigen presentations pathways may be overcome by NK cell-based immunotherapy. (elifesciences.org)
  • Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
  • Additionally, a comprehensive overview of antigen selection is presented along with preliminary results and future directions of CAR-T therapy development. (biomedcentral.com)
  • Histological analysis and CD31 immunostaining to quantify microvessel density (MVD) was performed on the explanted lung tissue of 13 transplanted patients. (ersjournals.com)