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  • induces
  • Our first demonstration that crosslinkage of TCR with the CD2 antigen induces proliferation of normal human CD4+ T cells and CD8+ T cells, in addition to revealing a novel activation mechanism utilizable by the two major subsets of T cells, suggest that the CD2 antigen might be targeted for the regulation of antigen-specific T cell immunity (e.g., organ transplantation). (rupress.org)
  • Activation of T cell antigen receptor (TCR) induces tyrosine phosphorylations that mediate the assembly of signaling protein complexes. (nih.gov)
  • Treatment with polyclonal antilymphocyte globulins induces depletion of circulating CD3+CD4+CD28− T-cells in transplant recipients.Prevalences of peripheral circulating CD3+CD4+CD28− T-cells in 16 age- and sex-matched healthy controls, 5 allograft recipients before and 6 hours after the application of ATG-F and 11 control patients before and 6 hours after organ transplantation. (nih.gov)
  • immunoisolates
  • 1999). α-CD3 immunoisolates from ANJ3 contained TCR ζ-chain, albeit at a reduced immunoisolation efficiency, but lacked LAT and associated PLC-γ and Grb2. (nih.gov)
  • In contrast, a LAT variant mutated in the S-palmitoylation sites Cys 26 and Cys 29 was not recruited into the α-CD3 isolates and failed to reconstitute PLC-γ and Grb2 recruitment into α-CD3 immunoisolates. (nih.gov)
  • B) Effect of cholesterol depletions on recovery of signaling protein in α-CD3 immunoisolates. (nih.gov)
  • immunoisolation
  • LAT-deficient ANJ3 cells and ANJ3 cells reconstituted with WT or palmitoylation-deficient C26/29A LAT were used for α-CD3 immunoisolation. (nih.gov)
  • LAT-deficient JCaM2 cells, JCaM2 expressing wt LAT, and Lck-LAT were subjected to α-CD3 immunoisolation. (nih.gov)
  • gene
  • In contrast, triggering of their CD3-Ti components resulted in the normal set of T lymphocyte-associated activation events, including phosphoinositide turnover, elevation in intracellular free calcium, early gene-induction events, and IL-2 production. (rupress.org)
  • cytokines
  • Peripheral blood mononuclear cells (PBMC) were activated in vitro by anti-CD3, and cytokines were quantified in supernatants. (nih.gov)
  • activation
  • A novel model for antigen-dependent activation of normal human T cells. (rupress.org)
  • All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. (uniprot.org)
  • complexes
  • We employed a novel method to immunoisolate plasma membrane subfragments that were highly concentrated in activated TCR-CD3 complexes and associated signaling proteins. (nih.gov)
  • cells
  • Results Our artificial antigen-presenting cells expanded both polyclonal T cells and MART-1-specific CD8 + T cells in a more efficient manner than the other systems. (haematologica.org)
  • Stimulation with artificial antigen-presenting cells allows for the generation of viable T cells displaying an immunophenotype consistent with in vivo potential for persistence, without increasing the frequency of regulatory T cells. (haematologica.org)
  • The starting specificity of anti MART-1 CD8 + T cells was preserved after stimulation with artificial antigen-presenting cells and it was statistically greater when compared to the activity of the same cells expanded with the other systems. (haematologica.org)
  • Finally, our artificial antigen-presenting cells proved to be suitable for large-scale application, minimizing the volume and the costs of T-cell expansion. (haematologica.org)
  • Conclusions Our artificial antigen-presenting cells might represent an efficient tool to rapidly obtain a sufficient number of functional T cells for adoptive immunotherapy in patients with cancer. (haematologica.org)
  • Transmembrane signaling of normal human T cells was explored with mAbs directed at TCR, CD2, CD4, CD5, or CD8 antigens and highly purified CD4+ T cells and CD8+ T cells. (rupress.org)
  • Detection of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/ml. (novusbio.com)
  • Importantly, LAT, PLC-γ, and Grb2 were efficiently coisolated in α-CD3-immunoisolated PM from wt LAT-reconstituted ANJ3 cells. (nih.gov)
  • protein
  • In this study, we reveal that the human CD8 antigen is also associated with the T-cell-specific protein-tyrosine kinase (p56lck). (pnas.org)
  • Two-dimensional nonequilibrium pH-gradient gel electrophoresis and sodium dodecyl sulfate/polyacrylamide gel electrophoresis demonstrated the similarity of p56lck to the protein-tyrosine kinase associated with the CD4 antigen. (pnas.org)
  • polyclonal
  • We also assessed the expression of CD40L on polyclonal NK effectors derived from peripheral blood of normal donors, by double staining with anti-CD40L mAb TRAP-1 and anti-CD3 or anti-CD56 mAb. (nih.gov)