CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α X and CR4. (biolegend.com)
CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. (biolegend.com)
Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. (thermofisher.com)
CD11b and CD11c3
In particular, CD11 is composed of CD11a, CD11b and CD11c. (thermofisher.com)
What is the difference between CD11b and CD11c? (studybuff.com)
BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry. (cytekbio.com)
Among them, 2 subsets of competent antigen presenting cells are demonstrated with distinct functional characteristics, one being DCs and the other being MHCII+CD11c+CD115+CD14-CD206- cells. (nih.gov)
DCs are able to promote fully activated T cells and superior in expanding cytokine producing inflammatory T cells, whereas MHCII+CD11c+CD115+CD14-CD206- cells generate partially activated T cells and possess a greater ability to induce regulatory T cells under TGF-β and retinoic acid conditions. (nih.gov)
While the development of DCs and MHCII+CD11c+CD115+CD14-CD206- cells are responsive to the treatment of FLT3L and GM-CSF, the numbers of LPMs, SPMs, and MHCII+CD11c+CD115+CD14+CD206+ cells are only influenced by the injection of GM-CSF. (nih.gov)
In addition, the analysis of transcriptomes reveals that the gene expression profile of MHCII+CD11c+CD115+CD14+CD206+ cells share high similarity with that of SPMs. (nih.gov)
Collectively, our study identifies 2 distinct subpopulations of MHCII+CD11c+CD115+ cells, (i) MHCII+CD11c+CD115+CD14-CD206- cells closely related to DCs and (ii) MHCII+CD11c+CD115+CD14+CD206+ cells to SPMs. (nih.gov)
Cluster of differentiation 14 (CD14) was described as monocyte/ macrophage differentiation antigen on the surface of myeloid lineage, such as monocytes, macrophages and dendritic cells (DCs). (studybuff.com)
Specialized antigen prese2
Dendritic cells (DCs) are specialized antigen presenting cells that instruct T cell responses through sensing environmental and inflammatory danger signals. (frontiersin.org)
Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)
Subsets4
In mice, cDCs (CD11c + MHCII + ) are sub-divided into functionally distinct phenotypes defined as cDC1 (CD8 + IRF8 + XCR1 + Clec9a + CD24 + ) and cDC2 (IRF4 + CD11b + SIRPα + ) whilst in humans the equivalent DC subsets are defined by expression of CD8 + IRF8 + XCR1 + Clec9a + CD141 + (cDC1) and IRF4 + CD1c + (cDC2) ( 1 - 4 ). (frontiersin.org)
Considering phenotypical, functional, and ontogenic features, peritoneal myeloid mononuclear cells are divided into 5 subsets: large peritoneal macrophages (LPMs), small peritoneal macrophages (SPMs), DCs, and 2 MHCII+CD11c+CD115+ subpopulations (i.e. (nih.gov)
Distinct dynamics of antigen-specific induction and differentiation of different CD11c + Tbet + B-cell subsets. (bvsalud.org)
Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. (cytekbio.com)
CD11a2
CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
CD18 non-covalently associates with CD11a, CD11b or CD11c. (angioproteomie.com)
CD181
CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. (thermofisher.com)
MHCII1
Intraepithelial FMDV-negative, MHCII/CD11c-double-positive dendritic cells were present in close proximity to FMDV-positive cells, the researchers said. (thebeefsite.com)
Dendritic cell4
In humans, pDCs exhibit plasma cell morphology and express CD4, HLA-DR, CD123, blood-derived dendritic cell antigen-2 (BDCA-2), Toll-like receptor (TLR) 7 and TLR9 within endosomal compartments. (wikipedia.org)
These cells have a plasmacytoid dendritic cell phenotype, a distinct response to TLR ligation and present antigen to T cells far less efficiently on comparison to conventional CD11c(hi) dendritic cells. (cam.ac.uk)
The aim of the project was to investigate how CD11c is distributed in the blood, how 2 CD11c antibody clones affect dendritic cell maturation and if they are internalized by the dendritic cells. (aau.dk)
At early time points after aerosol inoculation, viral antigens were found together with cytokeratin-positive pharyngeal epithelial cells. (thebeefsite.com)
As immunization with the optimal dose of the dectin-2 lentivector was similar to that stimulated by a lentivector containing a strong constitutive viral promoter, targeting antigen expression to dendritic cells can provide a safe and effective vaccine. (ox.ac.uk)
Cross-presentation initiates immune responses against tumors and viral infections by presenting extracellular antigen on MHC I to activate CD8 + T cell-mediated cytotoxicity. (elsevierpure.com)
Furthermore, diabetic VAT was enriched for CD206+CD11c− ATM and inflammatory signatures, scavenger receptors, and MHC II antigen presentation genes. (jci.org)
Cells44
Type I interferon signaling in dendritic cells and in nonhematopoietic cells selectively stimulates Tfh cell development in response to antigen in conjunction with Toll-like receptor (TLR)3 or TLR4 agonists. (nih.gov)
Yet, if and how the tissue environment shapes the antigen specificity of iNKT cells remains unknown. (elifesciences.org)
By analysing iNKT cells from lymphoid tissues of mice and humans we demonstrate that their T cell receptor (TCR) repertoire is highly diverse and is distinct for cells from various tissues resulting in differential lipid-antigen recognition. (elifesciences.org)
This suggests that unique tissue-specific factors (including local lipid antigens, cytokines and/or hormones) may shape the population of iNKT cells resident in those tissues, ultimately regulating local immune responses. (elifesciences.org)
iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
Despite lower co-stimulatory molecule expression, ProGP-4-generated Class II+/CD11c+ cells stimulated proliferation of allogeneic T cells and an antigen-specific T cell hybridoma as efficiently as bone marrow derived dendritic cells from untreated mice. (medscimonit.com)
Functionally, cDC1 cross-present exogenous antigens to activate CD8 + T cells and can promote IL-12 dependent Th1 responses ( 1 , 5 - 7 ). (frontiersin.org)
From here, the antigen is slowly released and provided to immune cells over an extended period of time. (mdpi.com)
Different from pre-cDCs, tDCs have less turnover, capture antigen, respond to stimuli and activate antigen-specific naive T cells, all characteristics of differentiated DCs. (stanford.edu)
I am interested in the mechanisms underlying this Foxp3+ regulatory T cell expansion in vivo and have focused on whether antigen presenting cells such as dendritic cells are re-programmed to a tolerogenic, regulatory T cell inducing, phenotype following infection. (cam.ac.uk)
I have found that chronic helminth infection is associated with the expansion of CD11c(lo) dendritic cells in the draining lymph nodes of the gut. (cam.ac.uk)
CD11c(lo) cells polarise naive T cells to distinct T cells lineages, encouraging the expansion of antigen-specific Foxp3 T cells and polarisation away from Th17 cells. (cam.ac.uk)
First discovered by Ralph Steinman and Zanvil A. Cohn in the 1970s, named for their tree-like branches or "dendrites", these cells patrol our bodies, ingest pathogens, process them, and present fragments (antigens) to T-cells, thus initiating a targeted immune response [2]. (origene.com)
cDC1: These cells are very effective at presenting antigens to CD8+ T cells, a type of cytotoxic T cell that can kill infected or cancerous cells. (origene.com)
cDC2: These cells are specialized in presenting antigens to CD4+ T cells, a type of helper T cell that helps coordinate the immune response. (origene.com)
Antiviral CD8 + T Cells Restricted by Human Leukocyte Antigen Class II Exist during Natural HIV Infection and Exhibit Clonal Expansion. (nationaljewish.org)
CD11c-Expressing B Cells Are Located at the T Cell/B Cell Border in Spleen and Are Potent APCs. (nationaljewish.org)
Immunization with a lentivector that targets tumor antigen expression to dendritic cells induces potent CD8+ and CD4+ T-cell responses. (ox.ac.uk)
To improve safety while retaining efficacy, we constructed a lentivector in which transgene expression was restricted to antigen-presenting cells using the mouse dectin-2 gene promoter. (ox.ac.uk)
In mice GFP expression was detected in splenic dectin-2(+) cells after intravenous injection and in CD11c(+) dendritic cells in the draining lymph node after subcutaneous injection. (ox.ac.uk)
Although T-cell A2BR plays an insignificant role for A2BR-mediated immunosuppression and tumor promotion, A2BR deficiency in tumor-bearing mice caused increased infiltration of myeloid and CD103+ dendritic cells, which was associated with more effective cross-priming of adoptively transferred tumor antigen-specific CD8+ T cells. (figshare.com)
A2BR deletion also intrinsically favored accumulation of myeloid and CD11bdim antigen-presenting cells (APC) in the tumor microenvironment. (figshare.com)
ATMs comprised CD11c + CD206 + cells in "crown" aggregates and solitary CD11c − CD206 + cells at adipocyte junctions. (diabetesjournals.org)
Tissue culture medium conditioned by CD11c + ATMs, but not CD11c − ATMs or other stromovascular cells, impaired insulin-stimulated glucose uptake by human adipocytes. (diabetesjournals.org)
CD11c has been proposed to function in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T cell proliferation by Langerhans cells. (studybuff.com)
Overall, CD11c was expressed in all B cell subpopulations, suggesting a role in all B-cell development steps, with an increased frequency in antigen-driven B cells, which accumulate with age of HD, independently of gender. (studybuff.com)
CD11c is a type I transmembrane protein that is expressed on monocytes, granulocytes, a subset of B cells, dendritic cells, and macrophages and may be demonstrated on B-cell chronic lymphocytic leukemia, marginal zone lymphomas, and hairy cell leukemia. (studybuff.com)
METHODS: Human peripheral blood mononuclear cells (PBMCs) were stimulated in vitro with HDI-albumin conjugates or control antigen, and changes in phenotype, gene and protein expression were characterized by flow cytometry, microarray, Western blot and ELISA. (cdc.gov)
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. (cytekbio.com)
Additionally, IFNγ induces the production of cytokines, Fc receptor, and adhesion molecules and up-regulates MHC class I and II antigen expression by antigen presenting cells during an immune response. (bioxcell.com)
Here we use the conditional in vivo ablation of CD11c high DC to investigate the role of these cells in T-independent type 2 immune responses. (uni-luebeck.de)
Invariant natural killer T (iNKT) cells are CD1d-restricted T cells, which respond rapidly to antigen recognition and promote development of anti-tumor immunity in many tumor models. (biomedcentral.com)
0.05), suggesting the selective elimination of DCs cross-presenting tumor-associated antigens by iNKT cells. (biomedcentral.com)
Dendritic cells have been studied intensely for a few decades for their excellent antigen-presenting capabilities, and lately the focus has been on the idea of utilizing them for therapeutic cancer vaccines. (aau.dk)
Previous studies have shown great potential of using the CD11c molecule as a target on the dendritic cells. (aau.dk)
Neither of the CD11c antibody clones induced maturation of dendritic cells. (aau.dk)
Molecules2
As mentioned earlier, maturation also induces the expression of both MHC Class I and Class II molecules in pDCs as well, which allows the cell to optimize its antigen-presenting abilities. (wikipedia.org)
The endocytic capacity and the expression of surface expression of mannose receptor C-type 1-like B, MHC-II, myeloid markers CD11c, DEC205, and co-stimulatory molecules CD40, CD80, CD86 by MoDCs at different maturation states are assessed by flow cytometry. (re-place.be)
Monocyte1
VAT CD206+CD11c− ATMs were increased in diabetic participants, were scavenger receptor-rich with low intracellular lipids, secreted proinflammatory cytokines, and diverged significantly from 2 CD11c+ ATM subtypes, which were lipid-laden, were lipid antigen presenting, and overlapped with monocyte signatures. (jci.org)
Lipid1
In addition, the machinery of CD11c + ATMs indicates they metabolize lipid and may initiate adaptive immune responses. (diabetesjournals.org)
CDCs2
pDCs express lower levels of CD11c than cDCs, and cDCs downregulate CD11c upon migration or activation. (studybuff.com)
Both myeloid-specific or CD11c-specific conditional deletion of A2BR delayed primary tumor growth. (figshare.com)
Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques. (wikidoc.org)
Macrophage1
It is now recognized that several non-DC myeloid populations express CD11c, including alveolar macrophages and resident (Ly6C low) monocytes and that some macrophage populations express both CD11c and MHC class II [8, 9]. (studybuff.com)
ATMs3
VAT immunostaining found CD206+CD11c- ATMs concentrated in vascularized lymphoid clusters adjacent to CD206-CD11c+ ATMs, while CD206+CD11c+ were distributed between adipocytes. (jci.org)
In addition, CD11c + ATMs were enriched for mitochondria and for RNA transcripts encoding mitochondrial, proteasomal, and lysosomal proteins, fatty acid metabolism enzymes, and T-cell chemoattractants, whereas CD11c − ATMs were enriched for transcripts involved in tissue maintenance and repair. (diabetesjournals.org)
These findings identify proinflammatory CD11c + ATMs as markers of insulin resistance in human obesity. (diabetesjournals.org)
CD2061
M1 macrophages were identified as F4/80-positive/CD11c-positive/CD206-negative and M2 macrophages were identified as F4/80-positive/CD11c-negative/CD206-positive. (studybuff.com)
Genes2
The most significant (P-values 0.007-0.05) changes in microarray gene expression were noted in lysosomal genes, especially peptidases and proton pumps involved in antigen processing. (cdc.gov)
Following antigen-induced maturation (e.g. (re-place.be)
Glycoprotein1
Carcinoembryonic antigen (CEA) is a glycoprotein involved in cell adhesion . (wikidoc.org)
Lymphoid1
They said that the earliest site of microscopic localisation of FMDV antigens was the lymphoid follicle-associated epithelium of the pharyngeal mucosa-associated lymphoid tissue of the nasopharynx at six hours after the aerosol inoculation. (thebeefsite.com)
Mice5
Spleens from ProGP treated mice were evaluated by immunocytochemistry and enriched CD11c+ populations were functionally assessed in a mixed lymphocyte assay and in an antigen dependent CTL assay. (medscimonit.com)
A dectin-2 lentivector encoding the human melanoma antigen NY-ESO-1 primed an NY-ESO-1-specific CD8(+) T-cell response in HLA-A2 transgenic mice and stimulated a CD4(+) T-cell response to a newly identified NY-ESO-1 epitope presented by H2 I-A(b). (ox.ac.uk)
To address this, we generated DC-specific Sec22b knockout (CD11c-Cre Sec22b fl/fl ) mice. (elsevierpure.com)
Following 26 minutes of kidney ischemia and 24 hours of kidney reperfusion, plasma creatinine levels were measured from vehicle- and ATL313-pretreated CD11c-Cre , CD11c-CreAdora2a fl/WT , and CD11c-CreAdora2a fl/fl mice. (jci.org)
Interestingly, we found plasma blasts to express CD11c and to be diphtheria toxin-sensitive in CD11c-diphtheria toxin receptor-transgenic mice, providing a unique tool for future analysis of in vivo aspects of plasma cell biology. (uni-luebeck.de)
Substances1
This "depot" was formed by associating the antigen with substances able to persist at the injection site, such as aluminum salts or emulsions. (mdpi.com)
Cell4
Despite this prototypical TCR repertoire gene usage, in recent years it has become apparent that there are variations within the iNKT cell repertoire that ultimately impact the antigen recognition capacity and consequently the functional outcomes during an immune response. (elifesciences.org)
These regulatory T cell changes could be inhibited with excess antigen or the retinoic acid receptor antagonist LE540 and are enhanced with recombinant TGF -beta. (cam.ac.uk)
Using assays to quantify CD11c-mediated cell adhesion, we demonstrate that CD11c recognizes ICAM-2 and VCAM-1. (studybuff.com)
APCs1
Cutting Edge: Roles for Batf3-Dependent APCs in the Rejection of Minor Histocompatibility Antigen-Mismatched Grafts. (nationaljewish.org)
Monoclonal1
The following product was used in this experiment: CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscience™ from Thermo Fisher Scientific, catalog # 25-0114-82, RRID AB_469590. (thermofisher.com)
Binds1
Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. (biolegend.com)
Vivo1
In vivo, HDI-exposed subjects exhibited a drastic increase in the percentage of PBMCs with the same HDI-albumin responsive phenotype characterized in vitro (HLA-DR(+)/CD11c(+) with altered light scatter properties). (cdc.gov)
Phenotype1
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
Expression2
Flow cytometric analysis of CD11c expression on mouse splenocytes. (bdbiosciences.com)
Hammarstrom S. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. (wikidoc.org)
Responses3
The mechanism by which vaccine adjuvants enhance immune responses has historically been considered to be the creation of an antigen depot. (mdpi.com)
Lentivectors stimulate potent immune responses to antigen transgenes and are being developed as novel genetic vaccines. (ox.ac.uk)
The purpose of the present development is to use avian MoDCs to implement a cellular platform to increase understanding of the immune responses induced by various antigens of interest (e.g. vaccine candidates) and evaluate their immunogenic potential. (re-place.be)
Adjuvants1
The identification of Pathogen-Associated Molecular Patterns (PAMPs) has greatly advanced our understanding of how adjuvants work beyond the simple concept of extended antigen release and has accelerated the development of novel adjuvants. (mdpi.com)
Presentation1
A2AR agonists had no effect on DC antigen presentation or on Tregs. (jci.org)
Conditional1
Myeloid, but not CD11c-specific conditional, depletion delayed lung metastasis. (figshare.com)
Tissue1
In obese women, CD11c + ATM density was greater in subcutaneous than omental adipose tissue and correlated with markers of insulin resistance. (diabetesjournals.org)