• By analysing iNKT cells from lymphoid tissues of mice and humans we demonstrate that their T cell receptor (TCR) repertoire is highly diverse and is distinct for cells from various tissues resulting in differential lipid-antigen recognition. (elifesciences.org)
  • A skewed preference for BV7 genes and sequential "G" amino acids motif is observed in complementarity-determining region-3 of T cell receptor-β. (nature.com)
  • Increased binding scores are observed in T-cell clones with complementarity-determining region-3 of T cell receptor-β with a "(G)GG" motif. (nature.com)
  • An individual T-cell has a specific complementarity-determining region 3 (CDR3) of T cell receptor (TCR)-α and -β, which is a result of the recombination of somatic TCR V-(D)-J genes and junction diversity. (nature.com)
  • Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4 + /CD8 + thymocytes that co-express CD45RO and IL-4 receptor. (rndsystems.com)
  • This receptor is expressed on activated CD4+and CD8+T cells and B cells. (abeomics.com)
  • The RMT4-53 monoclonal antibody reacts with mouse T cell immunoglobulin and mucin domain 4 (TIM-4), a phosphatidylserine-binding receptor and member of the Ig superfamily. (bioxcell.com)
  • The F31-5G3 monoclonal antibody reacts with mouse T cell immunoglobulin and mucin domain 4 (TIM-4) a phosphatidylserine-binding receptor and member of the Ig superfamily. (bioxcell.com)
  • gem homozygous receptor mutant HCs display normal cell viability, afferent synaptogenesis, and peripheral innervation, yet exhibit strongly reduced extracellular potentials (∼50% of wild-type potentials). (jneurosci.org)
  • Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
  • PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. (thermofisher.com)
  • Such immune dysfunction may be due to a disorder in thymic output function (in particular in young patients), which results in a lower level of naive T-cells in the peripheral blood available for an immune response to the proliferation and abnormal expression of the T cell receptor (TCR) repertoire. (biomedcentral.com)
  • There remains an urgent need for the noninvasive tracking of transfused chimeric antigen receptor (CAR) T cells to determine their biodistribution, viability, expansion, and antitumor functionality. (snmjournals.org)
  • Mice with C5a receptor-deficient dendritic cells promote induction of Treg and Th17 cells. (medscape.com)
  • The CR1 receptor preferentially binds C3b that is covalently attached to immune complexes, and it has a weaker affinity for bound C4b and iC3b. (medscape.com)
  • CR1 and CR2 have been shown to influence the immune environment in a B-cell receptor-independent manner. (medscape.com)
  • CR2/CD21 interacts with C3b degradation products C3dg and C3d and can act synergistically with the B-cell antigen receptor (BCR) in B-cell activation. (medscape.com)
  • CD161 molecules are C-type lectin-like receptors that can either activate (CD161a) or inhibit (CD161b) effector leucocyte responses, eg, cytotoxicity or cytokine production, against target cells which express C-type lectin-like related (Clr) molecules. (bdbiosciences.com)
  • We aim to elucidate factors in the solid tumor microenvironment that can inhibit CAR T cells and develop decoys/traps or signaling switch receptors to rescue/enhance their activity. (unil.ch)
  • These GPC2 antibodies can be used as either independent agents or targeting domains in recombinant immunotoxins (RITs), antibody-drug conjugates (ADCs), and chimeric antigen receptors (CARs). (nih.gov)
  • Partial interspecies cross-reactivity between murine cytokines/chemokines or MHC and co-stimulatory molecules with their human cognate receptors, might reduce the survival capacity of engrafted human cells, possibly affecting the decay of the latent reservoir. (biomedcentral.com)
  • T-cell activation and dysfunction relies on direct and modulated receptors. (biomedcentral.com)
  • Chimeric antigen receptors (CARs) endow T cells with antigen-specific recognition, activation, and proliferation independent of major histocompatibility complex ( 1 - 3 ). (snmjournals.org)
  • The density of CR1 receptors on cell surfaces varies with the cell type and with the activation of the cell for neutrophils and monocytes. (medscape.com)
  • Because of their high numbers, red blood cells express an average of 90% of the CR1 receptors despite their having a lower number of antigenic sites per cell. (medscape.com)
  • Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)
  • PRAME was first identified as a tumor-associated antigen through analysis of the specificity of tumor-reactive T-cell clones derived from a patient with metastatic cutaneous melanoma. (pipelinereview.com)
  • Yet, if and how the tissue environment shapes the antigen specificity of iNKT cells remains unknown. (elifesciences.org)
  • To bind a specific antigen with high specificity and affinity, antibodies form a complementary shape to the prospective surface patch of the antigen using complementarity determining regions (CDRs). (healthyconnectionsinc.com)
  • This retention is independent of the cell's viral antigenic specificity, but may be dependent on the phenotype of the clone. (psu.edu)
  • CD4 + CD28 null T cells in blood and synovial fluid showed specificity for HCMV antigens, and their presence was clearly associated with HCMV seropositivity but not with anti-citrullinated protein antibodies in the serum or synovial fluid, nor with erosive disease. (biomedcentral.com)
  • Furthermore, CD4 + CD28 null T cells isolated from the synovial fluid were investigated with regard to antigen specificity and selective recruitment to the joint. (biomedcentral.com)
  • To mimic the evolutionary process by which protein networks develop, we adopted the basic mechanism by which antibodies are produced against antigens. (healthyconnectionsinc.com)
  • During quick B-cell proliferation, random mutations are launched into the immunoglobulin sequences, and clones that communicate antibodies with high affinities are preferentially selected. (healthyconnectionsinc.com)
  • These complexes can be immunoextracted from cell lysatesusing monoclonal antibodies to either Pur alpha or Rb. (embl-heidelberg.de)
  • Using interferon-γ ELISpot, we observed that 78% of PCR-positive volunteers with undetectable antibodies showed T cell immunity against SARS-CoV-2. (cdc.gov)
  • These new antibodies have been shown to specifically target GPC2-expressing neuroblastoma, medulloblastoma, and retinoblastoma cell lines. (nih.gov)
  • In the present article we investigated the distribution of CD4 + CD28 null T cells in the synovial membrane, synovial fluid and peripheral blood of RA patients, and analysed the association with erosive disease and anti-citrullinated protein antibodies. (biomedcentral.com)
  • The association with erosive disease and the levels of antibodies to citrullinated peptides/antigens was examined. (biomedcentral.com)
  • It has been hypothesized that the initial antibody responses against parasite antigens upon first few Plasmodium falciparum infections is dominated by non-protective IgG2/IgG4 and IgM antibodies, which then gradually develop into protective response dominated by cytophilic IgG1 and IgG3 antibodies. (biomedcentral.com)
  • Naturally occurring IgG antibodies against P. falciparum blood-stage antigens were analysed from plasma samples collected from four groups of individuals differing in age and level of exposure to P. falciparum infections. (biomedcentral.com)
  • The number of discernable low molecular weight parasite antigens detected by different IgG subclass antibodies from each plasma sample was recorded. (biomedcentral.com)
  • IgG4 and IgM antibodies in plasma samples from all groups detected very few parasite antigens. (biomedcentral.com)
  • IgG2 antibodies from all groups detected a common pattern of high molecular weight parasite antigens. (biomedcentral.com)
  • It has been hypothesized that development of effective IgG-mediated anti-parasite immunity depends on the maturation of antibody responses, not only in terms of their antigen specificities and affinity maturation, but also in terms of class-switching implying that the progressive development of malaria immunity in older children can be attributed to a switch of anti-parasite antibodies from the non-cytophilic to the cytophilic subclasses [ 3 , 17 ]. (biomedcentral.com)
  • Consistent with this, iNKT cells from different organs show distinct basal activation, proliferation and clonal expansion. (elifesciences.org)
  • Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. (neobiotechnologies.com)
  • Nevertheless, in tumor cells, mutated TCS ERK 11e (VX-11e) (oncogenic) RAS preferentially promotes success and proliferation. (techblessing.com)
  • CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. (rndsystems.com)
  • TIM-4 is thought to enhance the engulfment of apoptotic cells and play a role in regulating T cell proliferation. (bioxcell.com)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
  • Furthermore, KIN17 is associated with cancer cell proliferation, migration, invasion and cell cycle regulation by regulating pathways including the p38 MAPK, NF‑κB‑Snail and TGF‑β/Smad2 signaling pathways. (spandidos-publications.com)
  • PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types. (lookformedical.com)
  • Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine-activated killer cells. (bdbiosciences.com)
  • CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. (rndsystems.com)
  • This protein is constitutively expressed in mature B cells, plasma cells and plasmacytoid dendritic cells, and in many other cells, it is only expressed as a response to stimuli from IFN pathway. (wikipedia.org)
  • These CD161 proteins form ~ 60 kDa homodimers that are expressed on natural killer cells and subsets of T lymphocytes, activated monocytes, and dendritic cells. (bdbiosciences.com)
  • TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. (neobiotechnologies.com)
  • CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. (biolegend.com)
  • Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)
  • T-cell immunoglobulin and mucin (TIM)-4, the only TIM protein not expressed on T cells, is found on macrophages and dendritic cells. (bioxcell.com)
  • Dendritic cells (DCs) are the central architects of the immune response, inducing inflammatory or tolerogenic immunity, dependent on their activation status. (bioxcell.com)
  • It is a single-chain membrane glycoprotein of approximately 200 kd that has 4 allotypic forms on myeloid cells (eg, erythrocytes, granulocytes, monocytes), lymphoid cells (mostly B cells), follicular dendritic cells, and glomerular podocytes. (medscape.com)
  • C3dg and C3d also can bind to the surface of bacteria and fungi, allowing CR2 on B cells and follicular dendritic cells to present the organisms for immune triggering. (medscape.com)
  • Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. (neobiotechnologies.com)
  • Theprotein binds preferentially to the purine-rich single strand of thiselement, PUR. (embl-heidelberg.de)
  • Advances in understanding underlying immune mechanisms are feasibale using a variety of candidate antigens, including attenuated live parasites, crude antigens, pure or recombinant Leishmania proteins, Leishmania genes encoding protective proteins, as well as immune system activators from the saliva of parasite vectors. (ac.ir)
  • Cloning, appearance, and purification of recombinant protein. (greatlakeshighereducationnow.com)
  • These analyses have mainly been carried out by ELISA using recombinant proteins or synthetic peptides usually representing subdomains of malarial proteins as test antigens. (biomedcentral.com)
  • It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC). (biolegend.com)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • We observed a similar frequency (80%) of T-cell immunity in convalescent donors with strong antibody responses but did not detect immunity in negative controls. (cdc.gov)
  • We decided to follow up with these volunteers by repeating antibody tests and by assessing T-cell immunity by enzyme-linked immunospot (ELISpot) assay for interferon-γ (IFN-γ). (cdc.gov)
  • A selecting of raised antibody amounts in indigenous sufferers would reveal repeated contact with nephritogenic antigens, leading to progressive renal disease and ESRF ultimately. (greatlakeshighereducationnow.com)
  • The mouse monoclonal PSA antibody (Dianova DIA-PSA, clone HAM18) was used at 1:100 and 1:800. (cancercurehere.com)
  • In the present study, there was no evidence for switching of antibody responses from non-cytophilic to cytophilic subclasses against blood-stage parasite antigens as a likely mechanism for induction of protective immunity against malaria. (biomedcentral.com)
  • Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. (thermofisher.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • The subclass profile of naturally occurring IgG responses has therefore been extensively studied for several major blood-stage malaria vaccine candidate antigens. (biomedcentral.com)
  • This suggests that unique tissue-specific factors (including local lipid antigens, cytokines and/or hormones) may shape the population of iNKT cells resident in those tissues, ultimately regulating local immune responses. (elifesciences.org)
  • We found that in mice that completed OIT successfully, desensitized mast cells (MCs) showed functionally beneficial alterations, such as increased induction of regulatory cytokines and enhanced expansion of regulatory T cells. (bioxcell.com)
  • During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
  • T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
  • However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
  • The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)
  • It also expressed on eosinophils, basophils, endothelial and epithelial cells. (techblessing.com)
  • The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. (rndsystems.com)
  • Cloned populations of antigen-specific, murine T-lymphocytes, maintained in long-term continuous culture, have been reported to exhibit functional activity upon adoptive transfer. (psu.edu)
  • We have optimized the transduction and expansion of murine CAR T cells for cancer immunotherapy (DOI: 10.1084/jem. (unil.ch)
  • Murine lung epithelial (MLE) cell lines were produced from lung tumors derived from transgenic mice bearing the viral oncogene, SV40 large T antigen, under transcriptional control of the promoter-enhancer region of the human surfactant protein C (SP-C) gene. (elsevierpure.com)
  • Cells were selected on the basis of increased murine cystic fibrosis transmembrane conductance regulator (mCFTR) mRNA content and were dilution cloned to produce distinct immortalized epithelial cell lines. (elsevierpure.com)
  • Murine PD-1 mRNA expression has been shown to be correlated with activation-induced apoptosis in a mouse T-cell hybridoma cell line and murine thymocytes [ 13 ]. (biomedcentral.com)
  • Cutaneous T-cell lymphomas (CTCLs) are malignancies of skin-homing lymphoid cells, that have up to now not been investigated thoroughly for common oncogenic mutations. (techblessing.com)
  • [ 6 ] The lymphomatous infiltrate in some bone marrow specimens from these AITL patients contained numerous small or scattered large B cells, and these cells resembled either benign lymphoid aggregates or T-cell-rich large B-cell lymphoma, respectively. (medscape.com)
  • Western Blot profiling of blood-stage parasite antigens displaying reactivity with individual plasma samples in terms of their subclass specificities was conducted. (biomedcentral.com)
  • Tetherin, also known as bone marrow stromal antigen 2, is a lipid raft associated protein that in humans is encoded by the BST2 gene. (wikipedia.org)
  • Tetherin is a human cellular protein which inhibits retrovirus infection by preventing the diffusion of virus particles after budding from infected cells. (wikipedia.org)
  • The stabilization of the protein through disulfide bond within the coiled coil region seems to be important in its function Tetherin is known to block many different types of enveloped viruses by tethering the budding virus like particles (VLPs) and inhibiting them from leaving the cell surface. (wikipedia.org)
  • Rat natural killer cell antigen, NKR-P1, related to C-type animal lectins is a carbohydrate-binding protein. (bdbiosciences.com)
  • Oligosaccharide ligands for NKR-P1 protein activate NK cells and cytotoxicity. (bdbiosciences.com)
  • At the protein level, it is upregulated following the activation of these cells. (neobiotechnologies.com)
  • Pur-alpha is a highly conserved, sequence-specific DNA- and RNA-binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. (embl-heidelberg.de)
  • Pur-alpha can influence viral interaction through functional associations, for example with the Tat protein and TAR RNA of HIV-1, and with large T-antigen and DNA regulatory regions of JC virus. (embl-heidelberg.de)
  • Human Pur alpha, expressed as a glutathioneS-transferase fusion protein, specifically binds to the hypophosphorylatedform of Rb with an affinity as high as that of SV40 large T-antigen. (embl-heidelberg.de)
  • Inthe absence of DNA, glutathione S-transferase-Pur alpha binds to p56RB, anNH2-terminal-truncated Rb protein purified from Escherichia coli,containing the T-antigen binding domain, to form multimeric complexes. (embl-heidelberg.de)
  • We report here the cloning and sequencing of a cDNA encodinga protein with strong affinity for the PUR element. (embl-heidelberg.de)
  • Glypican-2 (GPC2) is a cell surface protein that is preferentially expressed in pediatric cancers including neuroblastoma, which makes GPC2 an attractive candidate for targeted therapy. (nih.gov)
  • Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • In contrast, MLE cells, cloned on the basis of abundant surfactant protein mRNAs, expressed apoJ and mCFTR mRNAs at low levels. (elsevierpure.com)
  • KIN17, which is known as a DNA and RNA binding protein, is highly expressed in numerous types of human cancers and was discovered to participate in several vital cell behaviors, including DNA replication, damage repair, regulation of cell cycle and RNA processing. (spandidos-publications.com)
  • Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)
  • Such antigen preparations do not always accurately mimic native parasite protein conformations, including post-translational modifications. (biomedcentral.com)
  • DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. (snmjournals.org)
  • The RAS-RAF-MEK-ERK signaling pathway regulates cell reactions to environmental stimuli and takes on a crucial part in many malignancies.6 Thus, RAF and MEK are attractive therapeutic focuses on.7,8 RAS is a little guanine-nucleotide binding proteins that is mounted on the inner part from the plasma membrane. (techblessing.com)
  • Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
  • and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. (lookformedical.com)
  • Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. (lookformedical.com)
  • Proteins that control the CELL DIVISION CYCLE. (lookformedical.com)
  • Proteins found in the nucleus of a cell. (lookformedical.com)
  • Intro Cutaneous T-cell lymphomas (CTCLs) are uncommon malignancies of skin-homing T lymphocytes. (techblessing.com)
  • In scenario 2, all postoperatively were used but nodal position was excluded as this parameter was frequently lacking, in low quality malignancies preferentially. (cancercurehere.com)
  • The role of PD-1 and PD-L1 in T-cell immune suppression and the potential for immunotherapy via blocking PD-1 and PD-L1 in hematological malignancies are also reviewed. (biomedcentral.com)
  • Successful treatment of B-cell malignancies with CD19-CAR T cells was a milestone for this approach ( 4 , 5 ). (snmjournals.org)
  • The report includes a compilation of currently active projects in research and development of passive and active immunotherapy and adoptive cell therapy targeting PRAME. (pipelinereview.com)
  • Both clones promote recovery from lethal pulmonary influenza infection upon adoptive transfer. (psu.edu)
  • These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
  • A better and deeper understanding of immune reconstitution following allo-HCT is required for further clinical application of these adoptive T-cell therapies. (nature.com)
  • One clone was a Lyt-2 + , L3T4 - , class I MHC-restricted, cytolytic T-lymphocyte subset. (psu.edu)
  • The other clone was a Lyt-2 - , L3T4 + , Class II MHC-restricted clone of the helper/amplifier T-cell subset. (psu.edu)
  • Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)
  • Few data are currently available on the in vivo distribution of these homogenous, functional cell populations. (psu.edu)
  • We examined 2 cloned populations of influenza virus-specific T-lymphocytes. (psu.edu)
  • Using cell populations labeled with [ 3 H]thymidine, we examined the distribution of these cells in tissue sections from various organs. (psu.edu)
  • These cloned cell populations were preferentially retained in the lungs of uninfected and influenza-virus-infected animals. (psu.edu)
  • Expanded populations of CD4 + T cells lacking the co-stimulatory molecule CD28 (CD4 + CD28 null T cells) have been reported in several inflammatory disorders. (biomedcentral.com)
  • Selective T cell ablation in vivo with 177 Lu-AABD seems feasible for clustered T-cell populations. (snmjournals.org)
  • Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. (degruyter.com)
  • PRAME (preferentially expressed antigen in melanoma), a member of the cancer-testis antigen family, has been shown to have increased expression in solid tumors. (pipelinereview.com)
  • BRAFV600E mutation however, not in wild-type BRAF or tumors having a KRAS mutation.12,14,15 Further, dealing with wild-type BRAF tumors with BRAFV600E specific inhibitors induced tumor growth in vitro and in vivo.14 Thus, MEK inhibitors may be appealing in wild-type BRAF cells. (techblessing.com)
  • Neoantigen-specific CD8 T cells with high structural avidity preferentially reside in and eliminate tumors. (unil.ch)
  • High avidity T cells (Tet hi ), if present, were only found in progressing PD-1 refractory tumors. (bmj.com)
  • However, the success of CAR T-cell therapy for solid tumors has been limited so far, and the mechanisms for the tumor's treatment resistance are not well understood. (snmjournals.org)
  • Since iNKT cell activation can prevent or promote immunopathology in diverse disease contexts, the strict control of peripheral iNKT cell homeostasis is vital to regulate local immunity. (elifesciences.org)
  • Beyond the common features shared by all iNKT cells (including their CD1-restriction and innate-like properties), cells found in discrete tissues have distinct phenotypes and functions that critically modulate the outcome of immunity ( Crosby and Kronenberg, 2018 ). (elifesciences.org)
  • The identification of CMV-CTL clones is one method used to assess cellular immunity against CMV. (nature.com)
  • 12. Scott P. Development and regulation of cell-mediated immunity in experimental leishmaniasis. (ac.ir)
  • In the context of cancer, appropriately activated DCs can induce anti-tumor immunity by activating innate immune cells and tumor-specific lymphocytes that target cancer cells. (mdpi.com)
  • We concluded that, in convalescent patients with undetectable SARS-CoV-2 IgG, immunity may be mediated through T cells. (cdc.gov)
  • speculated that T-cell immunity could also be protective against infection with SARS-CoV-2 ( 5 ). (cdc.gov)
  • We furthermore conducted follow-up testing for immunity to SARS-CoV-2 until a median of 75 days (range 24-154) after the onset of symptoms and correlated results of SARS-CoV-2-specific T- and B-cell immunity. (cdc.gov)
  • 2020). "Niche-Specific Reprogramming of Epigenetic Landscapes Drives Myeloid Cell Diversity in Nonalcoholic Steatohepatitis" Immunity 52(6): 1057-1074 e1057. (bioxcell.com)
  • MLE-13a3 cell lines expressing high levels of mCFTR mRNA also expressed apolipoprotein J (apoJ) mRNA, a developmentally regulated glycoprotein expressed preferentially in fetal lung. (elsevierpure.com)
  • In conclusion, although the mutations c.-307T>C and -307_-320del lead to a comparably strong increase in endothelial Ovol2 expression as seen in PPCD1 patients, endothelial dystrophy was not observed in the mouse model, implicating species-specific differences in endothelial cell biology. (bvsalud.org)
  • [ 2 ] It has become clear that AITL is a clonal T-cell disorder involving deregulation of B-cells and endothelial cells against a background involving a unique malignant microenvironment. (medscape.com)
  • Dunleavy et al noted that overexpression of the chemokine CXCL13 and vascular endothelial growth factor-A in AITL suggests that it may be derived from follicular helper T cells. (medscape.com)
  • Rb binding involves a Pur alpha region with limited homologyto the Rb-binding region of SV40 large T-antigen. (embl-heidelberg.de)
  • When animals are exposed to an antigen, B cells that express a low-affinity surface immunoglobulin are selected. (healthyconnectionsinc.com)
  • Conclusions Targeting subdominant T cell responses with lower avidity against pMHC affinity neoepitopes showed potential for improving PD-1 immunotherapy. (bmj.com)
  • It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy. (neobiotechnologies.com)
  • Significantly, RITs and CARs (using the CT3 clone) have shown specific killing activity against GPC2-positive neuroblastoma cells, strongly supporting that these candidates may be further developed as therapeutics for patients with GPC2-positive pediatric cancers. (nih.gov)
  • Kupffer cell loss was compensated by gain of adjacent monocyte-derived macrophages that exhibited convergent epigenomes, transcriptomes, and functions. (bioxcell.com)
  • In the synovial joint, infiltrating T cells are predominantly of the CD4 + phenotype and are often found in the proximity of B cells and macrophages. (biomedcentral.com)
  • Tissue homeostasis is critically dependent on the function of tissue-resident lymphocytes, including lipid-reactive invariant natural killer T (iNKT) cells. (elifesciences.org)
  • A significant number of non-neoplastic T cells (resting CD4 + T cells and activated small- or medium-sized CD8 + lymphocytes) may coexist with a minor neoplastic T-cell population. (medscape.com)
  • CD134 is expressed on activated CD4+ T lymphocytes, and its ligand, CD134L, is found preferentially on activated B cells. (abeomics.com)
  • Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)
  • Within peripheral tissues iNKT cell recent thymic emigrants exhibit a different TCR repertoire than mature cells, suggesting that the iNKT population is shaped after arrival to the periphery. (elifesciences.org)
  • Moreover, the iNKT cell TCR repertoire changes following immunisation and is shaped by age and environmental changes. (elifesciences.org)
  • Despite this prototypical TCR repertoire gene usage, in recent years it has become apparent that there are variations within the iNKT cell repertoire that ultimately impact the antigen recognition capacity and consequently the functional outcomes during an immune response. (elifesciences.org)
  • Here we show the features of repertoire diversity and the gene expression profile of HLA-A24 cytomegalovirus-specific cytotoxic T-cells in actual recipients according to the cytomegalovirus reactivation pattern. (nature.com)
  • TCR sequencing and cloning methods for repertoire analysis and isolation of tumor-reactive TCRs. (unil.ch)
  • 2014). "T-cell immunoglobulin and mucin domain 4 (TIM-4) signaling in innate immune-mediated liver ischemia-reperfusion injury" Hepatology 60(6): 2052-2064. (bioxcell.com)
  • However, the impact of omega-3 PUFAs on bone marrow adipose tissue (BMAT) and bone marrow stromal cell (BMSC) metabolism has not been intensively studied yet. (bvsalud.org)
  • 3 Using Open-Circuit Time Constants for the Approximate Determination of fH If the poles and zeros of the amplifier transfer function can be determined easily, and each type is specialized for a distinct shipping route within the cell, for a kind of cargo, or even for the storage of substances (e. brkers Using SDS-PAGE to separate components of a mixture. (topforex-brokers.com)
  • Background CD8 + T cells are a highly diverse population of cells with distinct phenotypic functions that can influence immunotherapy outcomes. (bmj.com)
  • However, the tumor microenvironment (TME) imposes different mechanisms that facilitate the impairment of DC functions, such as inefficient antigen presentation or polarization into immunosuppressive DCs. (mdpi.com)
  • NASH-induced changes in Kupffer cell enhancers were driven by AP-1 and EGR that reprogrammed LXR functions required for Kupffer cell identity and survival to instead drive a scar-associated macrophage phenotype. (bioxcell.com)
  • Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
  • It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. (neobiotechnologies.com)
  • AITL is a type of peripheral T-cell lymphoma (PTCL) that is clinically characterized by high fever and generalized lymphadenopathy. (medscape.com)
  • Although our systematic analysis yields no molecular traces of SARS-CoV-2 in the brain, we observe broad cellular perturbations indicating that barrier cells of the choroid plexus sense and relay peripheral inflammation into the brain and show that peripheral T cells infiltrate the parenchyma. (nature.com)
  • In rheumatoid arthritis, increased frequencies of CD4 + CD28 null T cells in peripheral blood have previously been associated with extra-articular manifestations and human cytomegalovirus (HCMV) infection, but their presence in and contribution to joint manifestations is not clear. (biomedcentral.com)
  • Strikingly, the dominant TCR-Vβ subsets of CD4 + CD28 null T cells in peripheral blood were often absent in synovial fluid. (biomedcentral.com)
  • We had a unique opportunity to investigate the presence of these CD4 + CD28 null T cells in the synovial membrane, the synovial fluid and peripheral blood from the same patients in a large cohort of RA patients. (biomedcentral.com)
  • Moreover, increasing data have shown that peripheral T-cell tolerance is an essential property of the specific immune response to tumor cells. (biomedcentral.com)
  • Single-cell RNA-sequence analyses demonstrate the homogenous distribution of the gene expression profile in individual cytomegalovirus-specific cytotoxic T-cells within each recipient. (nature.com)
  • On the other hand, bulk RNA-sequence analyses reveal that gene expression profiles among patients are different according to the cytomegalovirus reactivation pattern, and are associated with cytokine production or cell division. (nature.com)
  • However, little information is available on the gene expression profile (GEP) of CMV-CTL clones according to CMV reactivation patterns (and donor CMV serostatus) after allo-HCT. (nature.com)
  • The NASH diet induced significant changes in Kupffer cell enhancers and gene expression, resulting in partial loss of Kupffer cell identity, induction of Trem2 and Cd9 expression, and cell death. (bioxcell.com)
  • Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • the invention also provides non-human animals and cells comprising a transgene encoding an APP comprising the Swedish mutation and further comprising functionally disrupted endogenous APP gene loci, transgenes and targeting constructs used to produce such transgenic cells and animals, transgenes encoding human Swedish mutation APP polypeptide sequences, and methods for using the transgenic animals in pharmaceutical screening and as commercial research animals for modeling neurodegenerative diseases (e.g. (justia.com)
  • Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (abcam.com)
  • Slides were exposed and deparaffinized to heat-induced antigen retrieval for a quarter-hour in 98C in pH9.0 focus on retrieval solution (Agilent, Santa Clara, CA, USA) within a PT Link pre-treatment module (Agilent) and stained within an Autostainer Link 48 gadget (Agilent). (cancercurehere.com)
  • Functional characterization of high and low avidity GSW11-specific CD8 + T cells was conducted using flow cytometry and bulk RNA-seq. (bmj.com)
  • 2013). "Interruption of dendritic cell-mediated TIM-4 signaling induces regulatory T cells and promotes skin allograft survival" J Immunol 191(8): 4447-4455. (bioxcell.com)
  • In this study, we demonstrate that Ab blockade of DC-expressed TIM-4 leads to increased induction of induced regulatory T cells (iTregs) from naive CD4(+) T cells, both in vitro and in vivo . (bioxcell.com)
  • Importantly, these regulatory-T-cell-mediated inhibitions of allergic responses were dramatically decreased in mice lacking OIT-induced desensitized MC. (bioxcell.com)
  • Collectively, these findings show that the desensitization process modulates the activation of MCs, leading directly to enhanced induction of regulatory-T-cell expansion and promotion of clinical allergic unresponsiveness. (bioxcell.com)
  • These T cells could either represent cells potentiating the function of infiltrating leukocytes or represent suppressive regulatory T cells. (biomedcentral.com)
  • Subsequently, it has been shown that PRAME is expressed in many malignant neoplasms, including cutaneous melanoma, breast carcinoma, non-small cell lung cancer, and leukemia, whereas normal healthy tissues express minimal or no PRAME , with the exception of the testis and endometrium. (pipelinereview.com)
  • They are preferentially expressed by a variety of adenocarcinomas, including breast, prostate, ovarian and pancreatic carcinomas as well as malignant plasma cells of multiple myelomas. (sigmaaldrich.com)
  • Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)
  • CD9 antigen modulates cell adhesion, migration and platelet activation. (techblessing.com)
  • During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
  • When the presence of virus and viral components is detected by recognition molecules such as (RIG-I), a cascades of interactions happen between signaling molecules, eventually the signal reaches the nucleus to upregulate the expression of interferon-stimulated genes (ISGs), this in turn activates IFN-α pathway to send the signal to neighboring cells, which causes upregulation in the expression of other ISGs and many viral restriction factors, such as tetherin. (wikipedia.org)
  • iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
  • AITL is mainly derived from CD2 + CD3 + CD4 + CD5 + CD7 - mature T-helper cells with varying expression and partial loss of detectable CD4. (medscape.com)
  • Tet lo demonstrated precursor exhausted or progenitor T cell phenotypes marked by higher expression of Tcf-1 and T-bet, and lower expression of the exhaustion markers CD39, PD-1 and Eomes compared with Tet hi , whereas Tet hi cells were terminally exhausted. (bmj.com)
  • In addition, the low cytotoxicity of T-cells may be related to the high expression level of inhibitory molecules including programmed death-1 (PD-1), LAG-3 and NKG2A in CD8+T cells [ 11 ]. (biomedcentral.com)
  • Cell surface expression of DAbR1 was confirmed by cell uptake studies with radiolabeled AABD. (snmjournals.org)
  • Engagement of CD134 with its ligand, CD134L, delivers a strong costimulatory signal to effector T cells. (abeomics.com)
  • Moreover, it has been shown that the PD-1 ligand (PD-L1) is highly expressed in leukemia cells. (biomedcentral.com)
  • Knockdown of NRAS by siRNA induced apoptosis in mutant Hut78 cells however, not in CTCL cell lines missing RAS mutations. (techblessing.com)
  • In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
  • We previously established a direct single-cell analysis to simultaneously identify and quantify in vivo CMV-CTL clones after allo-HCT 9 , 10 . (nature.com)
  • CD134 is also involved in the stimulation of T cells, T dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. (abeomics.com)
  • In addition, the feasibility of imaging of DAbR1-positive T cells in vivo after intravenous injection of 86 Y/ 177 Lu-AABD was studied and radiation doses determined. (snmjournals.org)
  • Imaging studies showed 86 Y-AABD was retained by DAbR1-positive T cells while it continuously cleared from normal tissues, allowing for in vivo tracking of intravenously administered CAR T cells. (snmjournals.org)
  • We have demonstrated for the first time that T cells can be modified with DAbR1, enabling their in vivo tracking via PET and SPECT. (snmjournals.org)
  • Although Tetherin is localized to the lipid rafts on the surface of the cells, they are endocytosed to be sorted through TGN by clathrin-dependent pathway. (wikipedia.org)
  • Further insights on the roles of CD8 + specificities and TCR avidity of naturally arising tumor-specific T cells, where both high and low avidity T cells recognizing the same peptide-major histocompatibility complex (pMHC) coexist in the same tumor, are crucial for understanding T cell exhaustion and resistance to PD-1 immunotherapy. (bmj.com)
  • Tetramer staining was performed to determine the frequency and avidity of CD8 + T cells targeting the tumor-specific epitope GSW11 and confirmed with tetramer competition assays. (bmj.com)
  • Results Treatment success with anti-PD-1 was associated with the preferential expansion of low avidity (Tet lo ) GSW11-specific CD8 + T cells with Vβ TCR expressing clonotypes. (bmj.com)
  • Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. (biolegend.com)
  • CD4 + CD28 null T cells were infrequent in the synovial membrane and synovial fluid, despite significant frequencies in the circulation. (biomedcentral.com)
  • Thus, PRAME is a potential candidate for cancer immunotherapy because it is expressed by a variety of tumours and can induce T-cell immune responses. (pipelinereview.com)
  • Heterologous immunization with defined RNA and subunit vaccines enhances T cell responses that protect against Leishmania donovani. (ac.ir)
  • It remains unclear if the T-cell responses detected in SARS-CoV-2 IgG-negative persons without PCR-confirmed infection were induced by SARS-CoV-2 or prior infection with a different coronavirus. (cdc.gov)
  • Cytophilic IgG subclasses in plasma samples from individuals with higher levels of exposure to P. falciparum infections distinctly detected higher numbers of low molecular weight parasite antigens. (biomedcentral.com)
  • 1989) Cell 57:115-126 and Oltersdorf et al. (justia.com)
  • The initial reaction may be an unbalanced immune response to an unknown antigen. (medscape.com)
  • CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. (rndsystems.com)
  • Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively? (biolegend.com)
  • Opsonized immune complexes (coated by C3b and C4b) bind to CR1, mostly on red blood cells, and are cleared through the liver where they can be transferred to CR3-bearing phagocytes and endocytosed. (medscape.com)
  • Together these data imply a primary role for CD4 + CD28 null T cells in manifestations elsewhere than in the joints of patients with HCMV-seropositive rheumatoid arthritis. (biomedcentral.com)
  • They tend to enhance the effects of complement and are highly important in the binding of opsonized immune complexes on B cells. (medscape.com)
  • Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. (sigmaaldrich.com)
  • Krenacs et al have suggested that the phenotype of neoplastic cells in AITL appears consistent with the phenotype of activated follicular B-helper T cells. (medscape.com)
  • CD134 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. (abeomics.com)
  • The NRASQ61K mutation sensitized Hut78 cells toward development inhibition from the MEK inhibitors U0126, AZD6244, and PD0325901. (techblessing.com)
  • Based on their functional outcome, co-signaling molecules can be divided as co-stimulators and co-inhibitors, which positively and negatively control the priming, growth, differentiation and functional maturation of a T-cell response. (biomedcentral.com)
  • When the virion buds from the surface of the cell, one of the tetherin membrane domains is in the new viral membrane, the other remains in the plasma membrane, tethering the virion to the cell. (wikipedia.org)
  • Notably, we also found correlates between the frequency of CD4+ T-cells, their activation status and viral parameters, which were analogous to the ones in HIV-infected patients. (biomedcentral.com)
  • In particular, we quantified the HIV DNA in hu-mice treated for different length with cART and explored whether it correlated with the viral load before cART, the number of target cells and immune activation markers. (biomedcentral.com)