AntibodiesImmunotherapyLymphocytesExtracellularReceptorsVitroLymphoid cellsCarcinoembryonic AntigenVivoProliferationProteinsAntitumorReactiveMonoclonal antibodyDifferentiationReceptor T cellMiceCancersTissueCytotoxicPeptidesCytokinesColorectalLymphocyteTissuesRegulatoryClinicalGenesMelanomaProteinPhenotypicTumor cellAPCsMacrophageBoneMetastaticSecreteXenograftAdoptive TransferProgenitor CellsSpleenEpitopePancreaticImmunohistochemicalPeripheralRecognizeSurvivalMembraneTargetsHumanSplenocytesAntibody responseSurfaceTumorsCancerExpressionImmune responseMast cellsRecognitionInduceSpecificEndothelial cellsCytosolTherapiesSerum
Antibodies14
- SIGNIFICANCE STATEMENT Astrocytes are traditionally identified immunohistochemically by antibodies that target cell-specific antigens in the cytosol or plasma membrane. (jneurosci.org)
- The development of technology to produce monoclonal antibodies for the immunological detection of cell membrane antigens has been extensively applied to the identification of human lymphocyte cell surface antigens. (duke.edu)
- Monoclonal antibodies reactive with myeloid precursor cells in the bone marrow or cells undergoing early myeloid differentiation are capable of detecting antigens that may then appear at discrete stages of myeloid maturation or on cells from some patients with myeloid and/ or monocytic leukemia. (duke.edu)
- T cells provide protection to infection by cytokine-mediated mechanisms or through production of antibodies. (omicsonline.org)
- Experiments with B cell deficient mice have demonstrated that B cells and antibodies are essential for complete clearance of parasites from blood of the host [2]. (omicsonline.org)
- ABSTRACT We have raised a panel of 15 monoclonal antibodies (MAbs) recognizing cell surface antigens of the rat osteoblast-like cell line ROS 17/2.8. (docksci.com)
- Monoclonal antibodies (MAbs) have been used successfully to characterize the cell surface of T other cell types, such as o s t e o ~ l a s t s (and ~ ~ ~lymphoid ) and myeloid cells, and to study the lineage of cells of the hematopoietic system. (docksci.com)
- In this way it was hoped to select antibodies to antigens associated specifically with the OB phenotype and eliminate those of housekeeping or transformation antigens. (docksci.com)
- However, implanted mutant MPS VII mice developed antibodies against the murine beta-glucuronidase, demonstrating a potential obstacle in patients with a null mutation who react against the replaced enzyme as a foreign antigen. (mcmaster.ca)
- Kinnaert P, Pradier O, Bournonville B, Habrant C, Goldman M, Van Geertruyden N: Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal antibodies. (exbio.cz)
- cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
- Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
- Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
- Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
Immunotherapy11
- It is an attractive immunotherapy target since it is strongly expressed in melanoma and numerous other cancers, yet highly restricted in normal human tissues. (mskcc.org)
- Immunotherapy utilizing T cell immunity has become a new treatment to eliminate cancer cells. (biomedcentral.com)
- Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
- The majority of neuroblastoma patients who receive immunotherapy have been previously treated with cytotoxic chemotherapy, making it paramount to identify neuroblastoma-specific antigens that remain stable throughout standard treatment. (bvsalud.org)
- We describe a murine model for the study of immunotherapy in colorectal cancers. (ox.ac.uk)
- Six immunizations of LAK cells generated in media containing fetal calf serum abrogated the ability of mice to respond to IL-2 plus LAK immunotherapy in a peritoneal carcinomatosis model. (prinsesmaximacentrum.nl)
- These observations may be important in interpreting IL-2 plus LAK immunotherapy studies in murine models and may modify our attempts to treat cancer patients. (prinsesmaximacentrum.nl)
- Sugarbaker, Paul H. / Lymphokine-activated killer cell and interleukin-2 inhibitors : Their role in adoptive immunotherapy . (prinsesmaximacentrum.nl)
- Nevertheless, ex vivo activation with cytokines can restore cytolytic activity of NK cells against GB, indicating that NK cells have potential for adoptive immunotherapy of GB if potent cytotoxicity can be maintained in vivo . (frontiersin.org)
- Furthermore, similar to T cells, specific recognition and elimination of cancer cells by NK cells can be markedly enhanced through expression of chimeric antigen receptors (CARs), which provides an opportunity to generate NK-cell therapeutics of defined specificity for cancer immunotherapy. (frontiersin.org)
- We then outline preclinical approaches that employ CAR-NK cells for GB immunotherapy, and give an overview on the ongoing clinical development of ErbB2 (HER2)-specific CAR-NK cells currently applied in a phase I clinical trial in glioblastoma patients. (frontiersin.org)
Lymphocytes7
- Serum from these treated mice would cause lysis of LAK cells or cultured lymphocytes in the presence of complement. (prinsesmaximacentrum.nl)
- IL-27 binds to IL-27ra and gp-130 complex, which is expressed on multiple cell types, including T lymphocytes [ 2 ]. (hindawi.com)
- Physiologic dsDNA breaks are generated in a variety of normal cellular functions, including the RAG endonuclease-mediated rearrangement of antigen receptor genes during the normal development of lymphocytes. (biomedcentral.com)
- This process is initiated by RAG endonucleases while the lymphocytes are in the G1 phase of the cell cycle. (biomedcentral.com)
- The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. (exbio.cz)
- Kearse KP: Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. (exbio.cz)
- Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
Extracellular6
- The PLAU gene encodes a serine protease (EC 3.4.21.73) involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. (wikipedia.org)
- In the extracellular matrix, urokinase is tethered to the cell membrane by its interaction to the urokinase receptor. (wikipedia.org)
- Glycoprotein antigens on the extracellular surface of the RBC membrane determine an individual's blood group. (cap.org)
- However, despite identification of ligands in extracellular matrix and cells for other GPCRs [2], F4/80 remains an orphan receptor. (ox.ac.uk)
- Multiplexed immunofluorescent imaging will map cell interaction partners of F4/80+ macrophages, and identify components of the extracellular matrix that interact with these cells. (ox.ac.uk)
- The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). (exbio.cz)
Receptors8
- Background Autologous patient T cells engineered to express antitumor T cell receptors (TCRs) and chimeric antigen receptors (CARs) have been effective for the treatment of certain cancer types, 1-4 and tumor neoantigens encoded by cancer-specific mutations have emerged as major targets of CD4+ and CD8+ T cells in immune checkpoint blockade (ICB) and in adoptive cell therapy (ACT). (bmj.com)
- In addition to specialised phagocytic receptors to recognize and clear senescent, apoptotic and necrotic cells and microbes, they are potent secretory cells able to nourish or destroy abnormal host cells and microbial targets, depending on their functional activation state. (ox.ac.uk)
- These experiments suggest that effects of androgens on developing B cells are mediated through androgen receptors in bone marrow stromal cells. (jci.org)
- Normal neuromuscular junction showing a presynaptic terminal with a motor nerve ending in an enlargement (bouton terminale): Synaptic cleft and postsynaptic membrane with multiple folds and embedded with several acetylcholine receptors. (medscape.com)
- These cells exhibit elevated AP activity modulated by PTH and dexamethasone, receptors for ~ Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, Birmingham, B15 2TH, England. (docksci.com)
- These breaks are necessary to create the vast diversity seen in lymphocyte antigen receptors. (biomedcentral.com)
- NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)
- 1999. Transactivation activity of human, zebrafish, and rainbow trout aryl hydrocarbon receptors expressed in COS-7 cells: Greater insight into species differences in toxic potency of polychlorinated dibenzo- p -dioxin, dibenzofuran, and biphenyl congeners. (cdc.gov)
Vitro9
- In vitro findings indicate that MSK's novel construct effectively retargets T-cells to CSPG4 (+) tumor cells. (mskcc.org)
- We developed an anti-PTK7 chimeric antigen receptor (CAR) and find PTK7 CAR T cells specifically target and kill PTK7-expressing neuroblastoma in vitro. (bvsalud.org)
- mAb 16.C8 mediated lysis of melanoma cells in vitro in antibody-dependent cellular cytotoxicity assays using human mononuclear effector cells isolated from normal volunteers or malignant melanoma patients. (duke.edu)
- We found that LAK cells cytolysis could not be generated if serum from IL-2-pretreated mice was present in vitro with splenocytes and IL-2. (prinsesmaximacentrum.nl)
- In vitro, these MAbs recognize cell surface antigens present on two additional cell lines, ROS 24/1 and Rat 2 cells, and on the adherent cell population cultured from rat long bone marrow. (docksci.com)
- Another four MAbs (UBIM 10, 11, 14, and 22) bind to a variety of cells and tissues both in vitro and in vivo. (docksci.com)
- Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (scancell.co.uk)
- However, PrP(0/0) mice do not show altered regulatory T-cell numbers or forkhead box P3 (Foxp3) expression levels, or impaired regulatory T-cell function in vitro. (ox.ac.uk)
- In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
Lymphoid cells4
- The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
- Genetic deletion of Il1a/b is associated with decreased recruitment of lymphoid cells and loss of interferon signaling in various immune populations and subsets of malignant cells and is associated with decreased survival time of PDGFB-driven tumor-bearing mice. (bvsalud.org)
- IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8. (biolegend.com)
- PrP(C) is expressed in lymphoid cells and is known to be a T-cell activation antigen. (ox.ac.uk)
Carcinoembryonic Antigen1
- Carcinoembryonic antigen (CEA) is a cell-surface glycoprotein that is expressed on normal human intestinal epithelium and that is overexpressed in intestinal tumors. (ox.ac.uk)
Vivo10
- Required for Treg generation in vivo in response to a model antigen introduced into the anterior chamber of the eye [3], F4/80 expression is also essential for organ transplantation [4] and engraftment of Lewis lung cancer cells. (ox.ac.uk)
- In vivo, human/murine binding PTK7 CAR T cells regress aggressive neuroblastoma metastatic mouse models and prolong survival with no toxicity. (bvsalud.org)
- This study has also identified cell surface antigens of ROS 17/2.8 cells that are not expressed by osteoblasts in vivo. (docksci.com)
- We have generated a panel of 15 MAbs to ROS 17/2.8 cells, 5 of which recognize antigens restricted to normal rat OBs and chondrocytes in vivo. (docksci.com)
- Importantly, in vivo the homocitrullinated aldolase specific response was associated with efficient CD8 dependent antitumor therapy of the aggressive murine B16 tumor model indicating that this epitope is naturally presented in the tumor. (scancell.co.uk)
- Here, we discuss effects of the GB tumor microenvironment on NK-cell functionality, summarize early treatment attempts with ex vivo activated NK cells, and describe relevant CAR target antigens validated with CAR-T cells. (frontiersin.org)
- Jacobs H, Vandeputte D, Tolkamp L, de Vries E, Borst J, Berns A: CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo. (exbio.cz)
- B cell subpopulations were studied by using B cell cloning procedures and an in vivo tolerance induction model. (usuhs.edu)
- Preclinical In Vivo Modeling of Cytokine Release Syndrome Induced by ErbB-Retargeted Human T Cells: Identifying a Window of Therapeutic Opportunity? (kcl.ac.uk)
- We used the in vivo rat heart model to study hMSC engraftment and retention in a normal beating heart. (ecu.edu)
Proliferation7
- MSK investigators have developed a fully humanized and potent bispecific antibody directed at Chondroitin Sulfate Proteoglycan 4 (CSPG4), which plays an important role in tumor cell proliferation and migration. (mskcc.org)
- Multiple myeloma (MM) is a hematological malignancy characterized by the proliferation of transformed monoclonal plasma cells in the bone marrow (BM) [ 1 ]. (biomedcentral.com)
- Meanwhile IL-27 exerts proinflammatory effects by promoting Th1, CD8, natural killer (NK), T follicular helper (Tfh), and B cell proliferation/functions and by inhibiting Treg and Th2 cell generation in parasites infection [ 4 ]. (hindawi.com)
- Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. (peprotech.com)
- Genotoxic damage causes robust alterations to pathways associated with B cell activation and increased proliferation, suggesting that genotoxic damage initiates not only the normal B cell maturation processes but also mimics activated B cell response to antigenic agents. (biomedcentral.com)
- CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. (exbio.cz)
- ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
Proteins4
- This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
- Here we show that PDGFB-driven GBM cells induce the expression of the potent pro-inflammatory cytokine IL-1ß in MDM, which engages IL-1R1 in tumor cells, activates the NF-kB pathway, and subsequently leads to induction of monocyte chemoattractant proteins (MCPs). (bvsalud.org)
- Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (scancell.co.uk)
- To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Antitumor6
- The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
- The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
- Unfortunately, repeated cycles of IL-2 plus LAK cells have not been demonstrated to exert additive antitumor effects. (prinsesmaximacentrum.nl)
- Homocitrullination occurs in the tumor microenvironment and CD4-mediated immune responses to Hcit epitopes can target stressed tumor cells and provide a potent antitumor response in mouse models. (scancell.co.uk)
- Human T4+ T cells exhibit antitumor activity against several ErbB+ cancer types. (kcl.ac.uk)
- Besides the isolation and identification of major histocompatibility complex I-restricted peptides from the surface of cancer cells, one of the challenges is eliciting an effective antitumor CD8+ T-cell-mediated response as part of therapeutic cancer vaccine. (elifesciences.org)
Reactive5
- 5-9 However, only a minority of intratumoral T cells are reactive to cancer antigens while the majority represent bystander cells. (bmj.com)
- 10-12 Conventional approaches to isolate tumor-reactive T cells and identify their TCRs from tumors rely on T cell function and can be impaired due to T cell exhaustion and dysfunction. (bmj.com)
- Expression of SOX9 did not decrease during aging and was instead upregulated by reactive astrocytes in a number of settings, including a murine model of amyotrophic lateral sclerosis (SOD1G93A), middle cerebral artery occlusion, and multiple mini-strokes. (jneurosci.org)
- The latter is a tool previously developed by Jacopo, 2020, able to identify tumor antigens similar to pathogen antigens in order to exploit molecular mimicry and tumor pathogen cross-reactive T cells in cancer vaccine development. (elifesciences.org)
- immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
Monoclonal antibody4
- This invention is a murine monoclonal antibody used to detect cell membrane antigens specific for human myeloid cells. (duke.edu)
- Scholars@Duke publication: Cell binding and tumor inhibiting functions of a new antihuman melanoma murine monoclonal antibody. (duke.edu)
- Murine, antihuman melanoma cell monoclonal antibody (mAb) 16.C8 was generated by fusing the murine myeloma cell line P3X63/Ag8.653 with splenocytes from a nude mouse bearing a human melanoma xenograft, after reconstitution with splenocytes from syngeneic immunocompetent BALB/c mice. (duke.edu)
- A mouse monoclonal antibody to rat major histocompatibility complex (MHC) class 1 antigens, MRC 0 x 1 8 , was purchased from Serotec (Oxfordshire, UK). (docksci.com)
Differentiation5
- Moreover, BCMA plays an essential role in regulating B cell maturation and differentiation into plasma cells. (biomedcentral.com)
- These antigens can thus be especially useful for the classification of leukemias and as a marker of myeloid cell differentiation. (duke.edu)
- Originally identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas, Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. (peprotech.com)
- Comparing these transcriptional responses provides a greater understanding of the mechanisms cells use in the differentiation between types of DNA damage and the potential consequences of different sources of damage. (biomedcentral.com)
- Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
Receptor T cell1
- BUFFALO, N.Y. - A new approach to chimeric antigen receptor T-cell (CAR T) therapy has shown great promise against small cell lung cancer (SCLC) in a preclinical study. (roswellpark.org)
Mice19
- The CD4(+) T cells expressed both cell surface and soluble TGF-beta and inhibited the development of an allergic phenotype when adoptively transferred to naive recipient mice. (nih.gov)
- While cells expressing cell surface TGF-beta were detectable in mice with inflammation, albeit at a lower frequency compared with that in tolerized mice, only those from tolerized mice expressed FOXP3. (nih.gov)
- Although cells that expressed TGF-beta on the cell surface (TGF-beta(+)), as well as the ones that did not (TGF-beta(-)), secreted equivalent levels of soluble TGF-beta, only the former were able to blunt the development of an allergic phenotype in mice. (nih.gov)
- Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
- Adhesion of macrophage cell lines from wild type and F4/80 knockout mice to purified matrix molecules, and complex 3D matrices, will be assessed, and binding sites mapped and downstream signalling examined. (ox.ac.uk)
- Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
- Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)
- The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)
- Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
- Androgens only affected B cell development in chimeric mice with androgen-sensitive stromal cells. (jci.org)
- Genetic loss or locally antagonizing IL-1ß/IL-1R1 leads to reduced MDM infiltration, diminished tumor growth, reduced exhausted CD8+ T cells, and thereby extends the survival of tumor-bearing mice. (bvsalud.org)
- Cell surface glycoproteomics performed on human-derived neuroblastoma tumors in mice following chemotherapy treatment identified protein tyrosine kinase 7 (PTK7) to be abundantly expressed. (bvsalud.org)
- Macroscopic and microscopic radioautographic analysis of the gastrointestinal tracts from MIN/CEA.Tg mice indicated that PR1A3 targeted and was retained in tumors at levels higher than in areas of normal gut. (ox.ac.uk)
- Kumar V, Rakha A, Saroa R, Bagai U (2015) CD4+T Cells Expansion in P. berghei (NK-65) Infected and Immunized BALB/C Mice. (omicsonline.org)
- We investigated the responses that repeated challenge with LAK cells and IL-2 provoked in normal BL/6 mice. (prinsesmaximacentrum.nl)
- Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion. (bdbiosciences.com)
- Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (scancell.co.uk)
- One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
- Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
Cancers5
- Malignant epithelial cancers, the major cause of cancer morbidity and mortality, arise in organs composed of both epithelial and mesenchyme-derived stromal cells, such as fibroblasts, myofibroblasts, endothelial cells, pericytes, smooth muscle, and hematopoietic cells. (aacrjournals.org)
- Although Mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural,pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometriod uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. (peprotech.com)
- The effectiveness of CAR T-cell therapy has so far been limited to blood cancers. (roswellpark.org)
- The new vaccine approach was found to be highly effective at pre-clinical stage in treating a range of cancers in murine models. (tcd.ie)
- that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
Tissue10
- Moreover, urokinase is used by normal cells for tissue remodeling and vessel growth, which necessitates distinguishing cancer-associated urokinase features for specific targeting. (wikipedia.org)
- uPA antigen is elevated in breast cancer tissue, which correlates with poor prognosis in breast cancer patients. (wikipedia.org)
- Cellular and matrix interactions of F4/80, an adhesion GPCR which defines murine tissue macrophages, in the normal and tumor microenvironment. (ox.ac.uk)
- The short range of α-particles also limits the absorbed dose to normal tissue adjacent to tumors compared with many electron emitters. (snmjournals.org)
- MAbs UBIM 9 and 21 bind to marrow cells in long bone sections, to the 7-day-old nonadherent cell population from cultured marrow, and to lymphoid tissue in sections of spleen. (docksci.com)
- Concomitantly, the intralysosomal accumulation of undegraded glycosaminoglycans was dramatically reduced in liver and spleen tissue sections and urinary glycosaminoglycan content was reduced to normal levels. (mcmaster.ca)
- however, the challenge for T cells is to recognize tumour antigens whilst minimizing cross-reactivity with antigens associated with healthy tissue. (scancell.co.uk)
- Other cancer indications and those with low mutational burden are less easily distinguished from normal tissue. (scancell.co.uk)
- Conclusions: The present study shows that CD276 can be used to discriminate ECs from malignant tissue from ECs from normal tissue. (researchgate.net)
- Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
Cytotoxic1
- Re-activating cytotoxic T cells after they have been disarmed by tumors has revolutionized cancer treatment. (ox.ac.uk)
Peptides2
- TCR's recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells (3, 4). (ancell.com)
- These peptides were then tested in our previously described oncolytic cancer vaccine platform PeptiCRAd, a vaccine platform that combines an immunogenic oncolytic adenovirus (OAd) coated with tumor antigen peptides. (elifesciences.org)
Cytokines8
- Depending upon the cytokines secreted by the Helper T cells, they are classified into two subpopulations: Th1 and Th2 cells. (omicsonline.org)
- Atherosclerosis, which is characterized by chronic inflammation in the arterial wall, is driven by immune cells and cytokines. (hindawi.com)
- Toxicity mediated by T4+ T cells results from target recognition in both tumor and healthy tissues, leading to release of both human (IL-2/IFN-γ) and murine (IL-6) cytokines. (kcl.ac.uk)
- During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
- T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
- However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
- The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)
- by normal individuals cause broncho-constriction in Cytokines often have overlapping biological activities, patients with asthma. (cdc.gov)
Colorectal1
- Antibody targeting studies in a transgenic murine model of spontaneous colorectal tumors. (ox.ac.uk)
Lymphocyte1
- B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
Tissues7
- Invasion and metastasis of malignant epithelial cells into normal tissues is accompanied by adaptive changes in the mesenchyme-derived supporting stroma of the target organs. (aacrjournals.org)
- BCMA is not present in other hematological cells like hematopoietic stem cells or other tissues. (biomedcentral.com)
- Furthermore, PTK7 shows minimal expression on pediatric-specific normal tissues. (bvsalud.org)
- Immunohistochemical studies of antigen localization on cryostat sections of rat calvaria, long bone, and soft tissues demonstrated that five of these MAbs, UBIM 1,2,3,12, and 17, recognize antigens that are restricted to normal rat osteoblasts and chondrocytes. (docksci.com)
- Studies of the interactions of this panel of MAbs with osteogenic tissues and cell lines may have an important impact on the understanding of osteoblast physiology. (docksci.com)
- While the biological functions of both Mesothelin and MPF remain speculative, high-levels of expression in cancerous tissues compared to limited distribution in normal tissues strongly suggests their involvement in tumorigenesis. (peprotech.com)
- genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
Regulatory10
- We propose a model of antigen-induced tolerance that involves cell-cell contact with regulatory CD4(+) T cells that coexpress membrane-bound TGF-beta and FOXP3. (nih.gov)
- CD4 + CD25 + FoxP3 + regulatory T cells (Tregs) are also involved in the mechanism of immune regulation against Plasmodium infection. (omicsonline.org)
- In the groups (both the initiation and the progression) receiving recombinant IL-27 administration, the formation of atherosclerotic plaques was suspended, and the percentage of regulatory T cells (LAP + or Foxp3 + ) in the spleen and peripheral blood was increased. (hindawi.com)
- Importantly, recent studies have shown that the importance of type 17 T helper cells (Th17 cells) and regulatory T cells (Tregs) in the pathogenesis of various immune disorders, particularly in atherosclerosis. (hindawi.com)
- They generate regulatory cells that inhibit the immune response that could potentially clear the tumours. (tcd.ie)
- The therapy is based on a combination of molecules that manipulates the immune response to curb the regulatory arm while enhancing the protective arm, allowing the induction of specialist white blood cell called killer T cells to target and eliminate the tumours. (tcd.ie)
- The cellular prion protein is preferentially expressed by CD4+ CD25+ Foxp3+ regulatory T cells. (ox.ac.uk)
- Further, transcription profiling studies of regulatory T cells have shown preferential overexpression of PrP(C), suggesting a possible role in regulatory function. (ox.ac.uk)
- We report that both the expression of PrP message and cell surface PrP(C) levels are increased in murine CD4(+) CD25(+) regulatory T cells compared with CD4(+) CD25(-) cells. (ox.ac.uk)
- Nevertheless, the preferential expression of surface PrP(C) by regulatory T cells raises the possibility that therapeutic ligation of PrP(C) might alter immune regulation. (ox.ac.uk)
Clinical5
- However, incompatibilities between the human and murine systems hamper clinical evaluation of these agents. (wikipedia.org)
- There is an unmet need to develop novel therapies for refractory/relapsed MM. In the past few years, chimeric antigen receptor (CAR)-modified T cell therapy for MM has shown promising efficacy in preclinical and clinical studies. (biomedcentral.com)
- Renier Brentjens, MD, PhD , Deputy Director and the Katherine Anne Gioia Endowed Chair in Cancer Medicine at Roswell Park Comprehensive Cancer Center, served as principal investigator of the study, "IL-18-secreting CAR T cells targeting DLL3 are highly effective in small cell lung cancer models," recently published in the Journal of Clinical Investigation. (roswellpark.org)
- Administration of T4+ T cells using the i.v. or intratumoral routes achieves partial tumor regression without clinical or histopathologic toxicity. (kcl.ac.uk)
- The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
Genes1
Melanoma4
- CSPG4 is an established melanoma-associated tumor antigen (also known as High-molecular-weight melanoma-associated antigen). (mskcc.org)
- In murine melanoma xenograft models, this bispecific antibody was shown to significantly inhibit tumor growth. (mskcc.org)
- Methods We performed single-cell RNA and T cell receptor (TCR) sequencing (scRNA/TCR-seq) on over 46,000 T cells isolated from eleven archival metastatic tumor samples whose primary cancer types included colon, rectal, breast, anal, and melanoma. (bmj.com)
- The antibody reacted strongly with fresh human melanoma cells and exhibited preferential reactivity with established human melanoma and neuroectodermal tumor cell lines. (duke.edu)
Protein6
- However, that's possible only when a specific type of receptor on the surface of the T cell fits with a specific antigen, or protein, on the surface of the cancer cell. (roswellpark.org)
- Then they went a step further, upgrading them to "armored CAR" T cells that could express another molecule - in this case, interleukin-18 (IL-18), a protein encoded by the IL 18 gene that regulates the immune system's response to disease. (roswellpark.org)
- Post-translational modification of the cellular prion protein (PrP(C)) is intimately associated with the pathogenesis of prion disease, yet the normal function of the protein remains unclear. (ox.ac.uk)
- Normal processing of amyloid precursor protein involves proteolytic cleavage at a site between residues Lys.sup.16 and Leu.sup.17 (as numbered for the .nu.AP region where Asp.sup.597 is residue 1 in Kang et al. (justia.com)
- This normal pathway cleaves within the region of the precursor protein which corresponds to the .beta. (justia.com)
- 19 normal healthy in- receptor protein (CD117) that is structurally dividuals were recruited as a control group. (who.int)
Phenotypic2
Tumor cell2
- He and his colleagues believe that ' s because the tumor microenvironment (TME) - the blood vessels, normal cells and other elements surrounding the tumor cell - suppresses the immune system, causing T cells to become exhausted and unable to defend against cancer cells. (roswellpark.org)
- 8000 peptide targets from the CT26 tumor cell line. (elifesciences.org)
APCs1
- We expressed predicted NeoTCRs in healthy donor PBL and screened them with antigen presenting cells (APCs) expressing candidate neoantigens. (bmj.com)
Macrophage2
- This project builds on the complementary research interests and expertise of Kim Midwood and Anja Schwenzer (Kennedy Institute) in Matrix Biology and Siamon Gordon (Dunn School), a macrophage cellular pathologist, whose group identified and characterized the F4/80 antigen, supplemented by the biochemical expertise of collaborator Hsi-Hsien Lin, (Chang Gung University, Taipei, Taiwan), who developed the F4/80 knockout mouse strain. (ox.ac.uk)
- Immunohistochemical analyses probed for the presence of human cells with human prolyl hydroxylase beta (p4hβ) and an immune response with murine monocyte/macrophage antigen (CD68). (ecu.edu)
Bone10
- This is especially important when considering the irradiation of the bone marrow, where the blood stem cells are situated. (snmjournals.org)
- Immature B cells from normal mouse bone marrow were not responsive to the suppressive effect of androgens unless they were cocultured with marrow stromal cells or with supernatants from androgen-treated stromal cells, suggesting that the androgen effects are exerted through marrow stromal elements by production of a diffusible mediator. (jci.org)
- Further experiments revealed that bone marrow stromal cells produced TGF-β in response to dihydrotestosterone (DHT), and neutralization of TGF-β in the DHT-treated stromal cells reversed the suppressive effects. (jci.org)
- Several lines of evidence support the hypothesis that gonadal steroids exert effects on the development of B cells in bone marrow. (jci.org)
- Androgens also exert effects on B cell development in bone marrow. (jci.org)
- The antigens appear to be localized to the cell surface of the osteoblast, with no apparent staining of bone matrix in either undecalcified or decalcified sections. (docksci.com)
- INTRODUCTION HE OSTEOBLAST (OB) is a key cell in bone formation, synthesizing and secreting the organic matrix of bone, and is involved in the regulation of matrix mineralization. (docksci.com)
- Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine interleukin-3 in murine bone marrow cell cultures. (peprotech.com)
- CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. (bdbiosciences.com)
- 15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
Metastatic3
- Contrast-enhanced CT scans taken in the portal venous phase show a hypervascular metastatic deposit from a renal cell carcinoma. (medscape.com)
- The patient had a previous right hepatectomy for an earlier renal cell solitary metastatic deposit. (medscape.com)
- demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer. (peprotech.com)
Secrete2
- A "universal" cell line engineered to secrete the missing enzyme is implanted in all recipients requiring the same enzyme replacement. (mcmaster.ca)
- Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)
Xenograft1
- In both xenograft and murine models of SCLC, the IL-18-secreting CAR T cells targeting DLL3 greatly increased anti-tumor activity, producing a stronger response than IL-12 and IL-16, two other interleukins evaluated during the study. (roswellpark.org)
Adoptive Transfer1
- Interleukin-2 (IL-2) and the adoptive transfer of lymphokine-activated killer (LAK) cells have been used to reduce tumor mass in intraperitoneal, pulmonary, and hepatic models. (prinsesmaximacentrum.nl)
Progenitor Cells1
- We found that SOX9 is almost exclusively expressed by astrocytes in the adult brain except for ependymal cells and in the neurogenic regions, where SOX9 is also expressed by neural progenitor cells. (jneurosci.org)
Spleen1
Epitope1
- Our results highlight the importance of the specific target antigen epitope in governing optimal CAR-T activity and provide a nanobody-based B7-H3 CAR-T product for use in solid tumor therapy. (nature.com)
Pancreatic1
- In the present study, we demonstrate that B7-H3 CAR-T cells targeting the IgC domain are more active than those targeting the IgV domain in pancreatic ductal adenocarcinoma (PDAC) and neuroblastoma (NB) mouse preclinical models. (nature.com)
Immunohistochemical1
- CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
Peripheral1
- Methods: Identification of tumour-associated endothelial markers (TEM) by comparing antigen expression on normal vs t-CEC and assess the presence of t-CEC in peripheral blood of cancer patients by incorporating TEM in our novel flow cytometry-based CEC detection assay. (researchgate.net)
Recognize2
- The ASH Giuseppe Bigi Memorial Award was established in 2015 to recognize an Italian trainee (undergraduate student, medical student, graduate student, resident physician, or post-doctoral fellow) based at an Italian institution who has the highest-scoring abstract submitted in the field of hematopoiesis and stem cells. (hematology.org)
- 2.8 cells recognize antigens, some of which are restricted to osteoblasts and chondrocytes. (docksci.com)
Survival3
- 12,13 Units prepared from this method, in follow-up studies in the early 1990s, were transfused to healthy volunteers with no ill effects and normal RBC circulatory survival times. (cap.org)
- The influx of monocytes/macrophages suggests an unfavorable micro-environment for exogenous stem cell survival, confirmed by the absence of human cells detected five days post injection. (ecu.edu)
- Unfortunately, this cell based vaccine only improves patient survival by an average of 4.1 months. (tcd.ie)
Membrane1
- 4.5)The object of our study was to raise MAbs to antigens restricted to the plasma membrane of the normal rat OB and to use these MAbs to further characterize the cell surface phenotype of the OB. (docksci.com)
Targets2
- Altered gene expression in these nontransformed stromal cells provides potential targets for therapy. (aacrjournals.org)
- Several antigens have been used as targets for CAR-T cell therapy against MM, including B cell maturation antigen (BCMA), CD19, CD138, signaling lymphocytic activation molecule 7 (SLAM7), and immunoglobulin light chains. (biomedcentral.com)
Human13
- Researchers are investigating several approaches, such as enzymatic treatments of RBCs, the generation of RBCs from human induced pluripotent stem cells, and the development of artificial oxygen carriers, all with the goal of advancing universal blood. (cap.org)
- In this study, we performed genetic manipulation of YAP in human-derived neuroblastoma cell lines to investigate YAP function in key aspects of the malignant phenotype, including mesenchymal properties, tumor growth, chemotherapy response, and MEK inhibitor response. (bvsalud.org)
- Human T cell receptor (TCR) is a T cell surface expressed heterodimer of alpha/beta or gamma/delta chains. (ancell.com)
- Antibody JOVI-3 is specific for the human V beta 3 TCR gene product and reacts with 1-10 of T cells in normal human blood (1, 5). (ancell.com)
- PeproTech's CHO cell-derived Recombinant Human MPF is the 250 amino acid-length fragment of the Mesothelin sequence, and has a calculated molecular weight of approximately 26.7 kDa. (peprotech.com)
- This further supports the hypothesis that there are different trafficking requirements for the development of murine and human iNKT cells, and a functional lysosomal/late-endosomal compartment is not required for human iNKT-cell development. (ox.ac.uk)
- We show that the human T1E28z CAR efficiently recognizes mouse ErbB+ cells, rendering this species suitable to evaluate preclinical toxicity. (kcl.ac.uk)
- Background: Human mesenchymal stem cells (hMSCs) show potential for therapeutic cellular cardiomyoplasty. (ecu.edu)
- In practice this has proven very difficult because unlike infectious diseases, tumours are derived from normal human cells, and not made up of foreign substances or antigens capable of triggering an immune response. (tcd.ie)
- PDF) Kraan J, van den Broek P, Verhoef C, Grunhagen DJ, Taal W, Gratama JW, Sleijfer SEndothelial CD276 (B7-H3) expression is increased in human malignancies and distinguishes between normal and tumour-derived circulating endothelial cells. (researchgate.net)
- The invention provides transgenic non-human animals, such as transgenic rodents and transgenic non-human mammalian cells harboring a transgene encoding an APP polypeptide comprising the Swedish mutation. (justia.com)
- the invention also provides non-human animals and cells comprising a transgene encoding an APP comprising the Swedish mutation and further comprising functionally disrupted endogenous APP gene loci, transgenes and targeting constructs used to produce such transgenic cells and animals, transgenes encoding human Swedish mutation APP polypeptide sequences, and methods for using the transgenic animals in pharmaceutical screening and as commercial research animals for modeling neurodegenerative diseases (e.g. (justia.com)
- Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
Splenocytes1
- Flow cytometry analysis (surface staining) of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE(red), and blank (black). (exbio.cz)
Antibody response1
- CD4 + T cells stimulate B cells to induce significant level of antimalarial antibody response and also help in the induction of CD8 + T-cell responses which help in arresting the growth of liver-stage parasites in the host. (omicsonline.org)
Surface6
- Targeting classic Tregs is limited by the detection of Foxp3 and surface Treg markers on activated cells. (hindawi.com)
- The availability of specific markers of the cell surface of the OB would be of great value in this task. (docksci.com)
- This technique has produced a MAb specific to the cell surface of the chick osteOcyte,'6) to the murine PTH receptor,") and to OB-restricted antigens. (docksci.com)
- First they developed CAR T cells that could home in on Delta-like ligand 3 (DLL3), an antigen found on the surface on SCLC tumor cells. (roswellpark.org)
- CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. (exbio.cz)
- Postexposure prophylaxis (PEP) with hepatitis B immune globulin (HBIG) and/or hepatitis B vaccine series should be considered for occupational exposures after evaluation of the hepatitis B surface antigen status of the source and the vaccination and vaccine-response status of the exposed person. (cdc.gov)
Tumors4
- However, the successful application of these emerging cell-based therapies in solid tumors remains limited. (nature.com)
- Since 2017 the Food & Drug Administration has approved six CAR T-cell products to treat those types of cancer, but the same treatment approach has fallen flat in patients with solid tumors. (roswellpark.org)
- First-generation CAR T cells have not yet demonstrated significant progress in solid tumors," says Dr. Brentjens. (roswellpark.org)
- While GB tumors are frequently infiltrated by natural killer (NK) cells, these are actively suppressed by the GB cells and the GB tumor microenvironment. (frontiersin.org)
Cancer7
- CAR T-cell therapy, sometimes called a "living drug," re-engineers a patient's own T cells, part of the immune system that can attach themselves to cancer cells and destroy them. (roswellpark.org)
- To ensure the right fit, T cells are separated from the patient's blood and sent to a laboratory, where a gene is inserted to transform them into CAR T cells that are better able to seek out, attach themselves to and kill cancer cells bearing a specific antigen. (roswellpark.org)
- These results suggest genotoxic damage may induce a unique cancer-prone phenotype and processes mimicking activated B cell response to antigenic agents, as well as the normal B cell maturation processes. (biomedcentral.com)
- Failure to properly repair this damage can lead to detrimental health effects, such as uncontrolled cell death and cancer formation. (biomedcentral.com)
- CEC measured in patients with advanced cancer are thought not only to derive from damaged normal vasculature (n-CEC), but also from damaged (t-CEC). (researchgate.net)
- Here, we describe for the first time a streamlined pipeline for the generation of personalized cancer vaccines starting from the isolation and selection of the most immunogenic peptide candidates expressed on the tumor cells and ending in the generation of efficient therapeutic oncolytic cancer vaccines. (elifesciences.org)
- Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
Expression5
- The directed expression of SV40 T antigen has led to the development of several important transgenic models with spontaneous epithelial tumor formation. (molvis.org)
- Transcriptome comparisons of SOX9+ cells with GLT1+ cells showed that the two populations of cells exhibit largely overlapping gene expression. (jneurosci.org)
- Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
- Results: No difference in antigen expression between normal and malignant endothelial cells (ECs) was found for CD54, CD109, CD137, CD141, CD144 and CXCR7. (researchgate.net)
- We aimed to determine the significance of detecting expression of some antigens that might be pivotal to the process, namely CD44 and CD117, in patients with active pemphigus vulgaris. (who.int)
Immune response1
- In Plasmodium chabaudi chabaudi (AS), T helper cells of host have been reported to be necessary for inducing a protective immune response against the blood stages of parasite [1]. (omicsonline.org)
Mast cells1
- Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)
Recognition2
- Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. (nature.com)
- The antibody selection procedure employed utilized a differential screening assay involving positive recognition of ROS 17/2.8 cells but not ROS 25/1 cells. (docksci.com)
Induce1
- It has been reported that IL-27 performs an anti-inflammatory function by acting on conventional CD4 + T cells to induce IL-10-producing cells that are implicated in controlling inflammatory responses [ 3 ]. (hindawi.com)
Specific11
- However, these MM-specific antigens have yet to be identified. (biomedcentral.com)
- Other workers have attempted to raise OB-specific MAbs using cells isolated from calvarial digests as immunogen. (docksci.com)
- Thus, Hcit-specific CD8 T-cell responses have potential in the development of future anticancer therapy. (scancell.co.uk)
- Some tumour cells, including those associated with viral infections, have clear, tumour-specific antigens that can be targeted by T cells. (scancell.co.uk)
- Hapten-specific murine colony-forming B cells. (usuhs.edu)
- Fluorescein- (FL) specific B cells from normal spleens were isolated by using FL gelatin plates and were then cultured in semisolid agar in the presence or absence of tolerogen. (usuhs.edu)
- Hapten-specific cells grew in soft agar to form discrete colonies. (usuhs.edu)
- These CFU-B could be triggered by a specific antigen to yield plaque-forming cells (PFC). (usuhs.edu)
- Dive into the research topics of 'Hapten-specific murine colony-forming B cells. (usuhs.edu)
- The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
- These results show that LMP1 , mour viruses classified by IARC in EBV-specific T-cell response. (who.int)
Endothelial cells2
- Background: Mature circulating endothelial cells (CEC) are surrogate markers of endothelial damage. (researchgate.net)
- Mature circulating endothelial cells (CEC) are surrogate markers of endothelial damage. (researchgate.net)
Cytosol1
- In normal control adult mouse eyes, ETS-1 was mostly present in the nuclei of all neuroretinal layers whereas ETS-2 was mostly localized in the cytosol of the cell bodies of these layers with a smaller amount present in the nuclei. (molvis.org)
Therapies1
- 3 Today, serologic and molecular techniques, along with laboratory information systems and electronic health records, contribute to precise blood product management and personalized transfusion therapies, particularly benefiting complex patients with sickle cell disease, thalassemia, and other diseases requiring chronic transfusion support. (cap.org)
Serum2
- LAK cells generated in the presence of normal mouse serum were not lysed. (prinsesmaximacentrum.nl)
- These data suggest that antibody against absorbed antigens on LAK cells and serum inhibitors of IL-2 are generated following repeated challenge. (prinsesmaximacentrum.nl)