• Disrupting the binding of CD47 to SIRPα has emerged as a promising immunotherapeutic strategy for advanced cancers by potentiating antibody-dependent cellular phagocytosis (ADCP) of targeted antibodies. (aacrjournals.org)
  • Murine SIRPα-Fc-CD40L outperformed CD47 blocking and CD40 agonist antibodies in murine CT26 tumor models and synergized with immune checkpoint blockade of PD-1 and CTLA4. (aacrjournals.org)
  • Antiviral activities of antibodies may either be dependent only on interactions between the antibody and cognate antigen, as in binding and neutralization of an infectious virion, or instead may require interactions between antibody-antigen immune complexes and immunoproteins or Fc receptor expressing immune effector cells. (frontiersin.org)
  • Similarly, CD47 −/− C57BL/6 mice were much more sensitive than their wild-type counterparts to experimental passive AIHA induced by anti-RBC monoclonal antibodies. (ashpublications.org)
  • Cross-reactivity with bovine cells of monoclonal antibodies submitted to the 6th International Workshop on Human Leukocyte Differentiation Antigens. (ox.ac.uk)
  • Twelve subpanels of monoclonal antibodies (MAb) included within the 6th International Workshop on Human Leukocyte Differentiation Antigens (6th HLDA) were assayed for reactivity with bovine peripheral blood leukocytes. (ox.ac.uk)
  • The glycoprotein CD47 (integrin-associated protein, IAP) is present on the surface of virtually all cells, including red blood cells (RBCs). (ashpublications.org)
  • CD47 acts like a marker of self by ligating the macrophage inhibitory receptor signal regulatory protein α (SIRPα). (ashpublications.org)
  • As proof-of-concept, the established target antigen CD44 was identified as a commonly and abundantly expressed surface protein with high endocytic activity. (lu.se)
  • Simultaneously, RRX-001 reduces CD47 protein levels, aiding in preventing immune escape by calreticulin-rich cancer cells. (bvsalud.org)
  • A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN . (nih.gov)
  • New data indicate that CXCR4 interacts with the surface protein CD47 in a novel form of immunosurveillance, called ImmunoGenic Surrender (IGS). (unisr.it)
  • Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. (exbio.cz)
  • While macrophage phagocytosis is an immune defense mechanism against invading cellular organisms, cancer cells expressing the CD47 ligand send forward signals to repel this engulfment. (bvsalud.org)
  • The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
  • The Rh blood group antigens (MIM 111700) are associated with human erythrocyte membrane proteins of approximately 30 kD, the so-called Rh30 polypeptides. (novusbio.com)
  • The Fc receptor-dependent function of antibody-dependent cellular phagocytosis (ADCP) provides mechanisms for clearance of virus and virus-infected cells, as well as for stimulation of downstream adaptive immune responses by facilitating antigen presentation, or by stimulating the secretion of inflammatory mediators. (frontiersin.org)
  • Fc receptor-dependent antibody functions are also involved in activation of downstream adaptive immune responses by facilitating antigen presentation or by stimulating the secretion of inflammatory mediators ( 12 , 13 ). (frontiersin.org)
  • Immunotherapies with antibody-drug-conjugates (ADC) and CAR-T cells, targeted at tumor surface antigens (surfaceome), currently revolutionize clinical oncology. (lu.se)
  • The efficacy was analogous or preferable to the combination of CAR T cells and CD47 antibody. (bvsalud.org)
  • Furthermore, locally delivering anti-CD47 by CAR T cells potentially limits toxicities relevant to systemic antibody treatment. (bvsalud.org)
  • Additionally, BMVs consist of considerable proteins, for example, original bacterial antigens and pathogen-associated molecular pattern components [ 5 ] . (encyclopedia.pub)
  • These data illustrated that whereas CD47/SIRPα inhibition could potentiate tumor cell phagocytosis, CD40-mediated activation of a type I interferon response provided a bridge between macrophage- and T-cell-mediated immunity that significantly enhanced durable tumor control and rejection. (aacrjournals.org)
  • Herein, we engineered CD19-CAR T cells to secrete an anti-CD47 single-chain variable fragment (scFv) and validated their function in enhancing antitumor efficacy, regulating T cells differentiation, modifying phagocytosis and polarization of macrophages by in vitro and in vivo researches. (bvsalud.org)
  • Although low CD47-expressing cancer cells still allow phagocytosis, the reverse signaling delays the process, leading to incomplete digestion of the entrapped cells and subsequent tumor hybrid cell (THC) formation. (bvsalud.org)
  • Notably, CXCR4/CXCL12 cross-signal via the T and B cell receptors (TCR and BCR) and co-internalize with CD47, promoting tumor cell phagocytosis by macrophages in an anti-tumor immune process called ImmunoGenic Surrender (IGS). (unisr.it)
  • SIRPα-Fc-CD40L bound CD47 and CD40 with high affinity and activated CD40 signaling in the absence of Fc receptor cross-linking. (aacrjournals.org)
  • A high recurrence rate of non-Hodgkin's lymphoma (NHL) following chimeric antigen receptor T (CAR T) cell treatment remains a bottleneck, and immunosuppressive tumor microenvironment (TME) compromising CAR T cell efficacy in NHL is the primary cause of relapse. (bvsalud.org)
  • Here we report that the reverse signaling using CD47 as a receptor additionally enhances a pro-survival function of prostate cancer cells under phagocytic attack. (bvsalud.org)
  • Tumor immunogenicity results from the processing, and subsequent presentation of tumor antigens released from dead or dying tumor cells on MHC molecules expressed on antigen-presenting cells (APC). (aacrjournals.org)
  • Following the co-internalization of CXCR4 and CD47 in tumor cells, macrophages phagocytose them and cross-present their antigens to the adaptive immune system, leading to tumor rejection in a fraction of mice. (unisr.it)
  • Using a layered double hydroxide nanosheet carrier, we co-deliver a T-type calcium channel inhibitor (TTA-Q6) and a CD47 inhibitor (RRX-001) into lung tumors. (bvsalud.org)
  • In this manner mild reactivity of wild-type RBCs with macrophage phagocytic receptors is tolerated, whereas otherwise identical CD47-deficient RBCs are rapidly eliminated. (ashpublications.org)
  • This phenotype is at least partially due to a markedly increased rate of elimination of opsonized CD47 −/− compared to CD47 + RBCs. (ashpublications.org)
  • The Rh antigens appear to exist as a multisubunit complex of CD47 (MIM 601028), LW (MIM 111250), glycophorin B (MIM 111740), and play a critical role in the Rh50 glycoprotein. (novusbio.com)
  • Surface calreticulin activates macrophages and triggers dendritic cell maturation, promoting effective antigen presentation and therefore activating antitumor T cells. (bvsalud.org)
  • The antiphagocytic activity of CD47/SIRPα serves as a macrophage checkpoint or "do not eat me" signal ( 1 ). (aacrjournals.org)
  • CD47 has been proved to be a promising therapeutic target and is crucial in regulating macrophage function. (bvsalud.org)
  • Of note, anti-CD47 scFv secreting CAR T cells exert a more potent immune response following specific antigen stimulation compared with parental CAR T cells, characterized by more efficient degranulation and cytokine production with polyfunctionality. (bvsalud.org)
  • Four of the MAb that belonged to CD23, CD39, CD47, and CDw149 stained bovine cells in a manner that indicated an almost identical cellular distribution of the antigen to that reported in humans. (ox.ac.uk)
  • These were investigated further by two-colour immunofluorescence to compare the cellular expression of the antigen on cattle cells with that reported for the different CD antigens in humans. (ox.ac.uk)
  • The CD47/SIRPα pathway is an endogenous pathway that facilitates clearance of aged or dying cells ( 1 ). (aacrjournals.org)
  • In addition, CXCL12 and CXCR4 are involved in antigen recognition by T and B cells and in shaping the tumor microenvironment (TME), mainly towards dampening immune responses. (unisr.it)
  • All of these specific activities of CXCL12 and CXCR4 in antigen presentation might be complementary to current immunotherapies. (unisr.it)
  • CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. (novoprolabs.com)
  • This graph shows the total number of publications written about "Antigens, CD47" by people in this website by year, and whether "Antigens, CD47" was a major or minor topic of these publications. (rush.edu)
  • Results show that the thrombospondin 1 (TSP1) and its receptor CD47 (CD47) axis selectively regulates NADPH oxidase 1 (Nox1) in the regulation of endothelial senescence and suggest potential targets for controlling the aging process at the molecular level. (cusabio.com)
  • Chimeric antigen receptor T cells in refractory B-cell lymphomas. (nature.com)
  • Beatty, G. L. & O'Hara, M. Chimeric antigen receptor-modified T cells for the treatment of solid tumors: defining the challenges and next steps. (nature.com)
  • Antiviral activities of antibodies may either be dependent only on interactions between the antibody and cognate antigen, as in binding and neutralization of an infectious virion, or instead may require interactions between antibody-antigen immune complexes and immunoproteins or Fc receptor expressing immune effector cells. (frontiersin.org)
  • The Fc receptor-dependent function of antibody-dependent cellular phagocytosis (ADCP) provides mechanisms for clearance of virus and virus-infected cells, as well as for stimulation of downstream adaptive immune responses by facilitating antigen presentation, or by stimulating the secretion of inflammatory mediators. (frontiersin.org)
  • Fc receptor-dependent antibody functions are also involved in activation of downstream adaptive immune responses by facilitating antigen presentation or by stimulating the secretion of inflammatory mediators ( 12 , 13 ). (frontiersin.org)
  • Dozens of BCMA-targeting therapies, such as antibody-drug conjugate, Bi-specific antibodies, and chimeric antigen receptor T (CAR-T) cells are presently being created and tested clinically. (news-medical.net)
  • The researchers found that anti-CD47 antibodies, which can block the "don't-eat-me" signal and enable macrophages to engulf cancer cells, eliminated or inhibited the growth of various blood cancers and solid tumors. (stanford.edu)
  • It was completely unexpected that CD8+ T cells would be mobilized when macrophages engulfed the cancer cells in the presence of CD47-blocking antibodies," said MD/PhD student Diane Tseng, the lead author of the study. (stanford.edu)
  • Because T cells are sensitized to attack a patient's particular cancer, the administration of CD47-blocking antibodies in a sense could act as a personalized vaccination against that cancer," Tseng added. (stanford.edu)
  • and virus infection, iron overload, and donor-specific anti-human leukocyte antigen antibodies to insects. (bvsalud.org)
  • Individuals lacking a specific Kell antigen may develop antibodies against Kell antigens when transfused with blood containing that antigen. (wikidoc.org)
  • Both AIHA and HDN may be severe when caused by anti-Kell antibodies, [10] as they are the most immunogenic antigens after those of the ABO and Rhesus blood group systems . (wikidoc.org)
  • When T cells are activated, they will express more immune checkpoint receptors, such as programmed cell death protein 1 (PD-1) or cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) [ 2 ] [ 3 ] . (encyclopedia.pub)
  • Phagocytes engulf foreign cells but not 'self' in part because self cells express CD47 as a ligand for signal regulatory protein SIRPalpha, which inhibits phagocytosis. (nih.gov)
  • Brooke G, Holbrook JD, Brown MH, Barclay AN: Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. (exbio.cz)
  • Current approaches to developing the vaccines rely on using immune cells called dendritic cells to introduce cancer protein fragments to T cells - a process known as antigen presentation. (stanford.edu)
  • To respond to coronavirus pandemic, ACROBiosystems has developed SARS-CoV-2 antigens specifically designed and optimized for serological test kits, including Spike-derived antigen S1, RBD and Nucleocapsid protein. (news-medical.net)
  • [1] [2] The Kell antigens are peptides found within the Kell protein , a 93- kilodalton transmembrane zinc -dependent endopeptidase which is responsible for cleaving endothelin-3 . (wikidoc.org)
  • The Kell glycoprotein links via a single disulfide bond to the XK membrane protein [6] that carries the Kx antigen . (wikidoc.org)
  • Absence of the XK protein (such as through genetic deletion or through a single point mutation within the coding region of the XK gene [8] ), leads to marked reduction of the Kell antigens on the red blood cell surface. (wikidoc.org)
  • Using immunoglobulin (IgG)-opsonized particles with varying levels of either human- or mouse-CD47, the effective inhibition constants K(i) for blocking phagocytosis are then determined with both human- and mouse-derived macrophages. (nih.gov)
  • CAR macrophages (CAR-Ms) demonstrated antigen-specific phagocytosis and tumor clearance in vitro. (nature.com)
  • Characterization of CAR-M activity showed that CAR-Ms expressed pro-inflammatory cytokines and chemokines, converted bystander M2 macrophages to M1, upregulated antigen presentation machinery, recruited and presented antigen to T cells and resisted the effects of immunosuppressive cytokines. (nature.com)
  • Importantly, we identified a subpopulation of M1 macrophages present in periodontally affected tissues which expressed high levels of CD47, a glycoprotein of the immunoglobulin family that plays a critical role in self-recognition and impairment of phagocytosis. (nih.gov)
  • Irving Weissman , MD, professor of pathology and of developmental biology , and his team previously showed that nearly all cancers use the molecule CD47 as a "don't-eat-me" signal to escape from being eaten and eliminated by macrophages. (stanford.edu)
  • The research revives interest in an aspect of macrophages that has been neglected for decades: their role in presenting antigens to T cells. (stanford.edu)
  • For many years, researchers have focused on the dendritic cell as the main antigen-presenting cell, and have generally believed that macrophages specialize in degrading antigens rather presenting them. (stanford.edu)
  • This research shows that macrophages can be effective at antigen presentation and are powerful initiators of the CD8+T cell response. (stanford.edu)
  • Development of reagents against leukocyte differentiation antigens in veterinary immunology is slower compared to humans and mice. (ox.ac.uk)
  • T cells can recognize differentiation antigens and other non-mutated self-antigens that are overexpressed by cancer cells in the context of sufficient inflammatory signals, which result from the release of damage-associated molecular pattern (DAMP) molecules [ 1 , 2 ]. (biomedcentral.com)
  • The mouse monoclonal antibody SE5A5 recognizes a common extracellular epitope on human CD172a and CD172b antigens (approx. (exbio.cz)
  • The fact that T cells become involved in fighting cancer as a result of CD47-blocking antibody therapy could have important clinical implications. (stanford.edu)
  • In total, we profiled the transcriptome and antigen levels of 7,097 cells using CITE-seq, with a median of 819 expressed genes and 3,235 antibody-derived tag (ADT) unique molecular identifiers (UMIs) per cell. (biorxiv.org)
  • Individuals without K antigens(K 0 ) who have formed an antibody to a K antigen, must be transfused with blood from donors who are also K 0 to prevent hemolysis. (wikidoc.org)
  • Autoimmune hemolytic anemia (AIHA) occurs when the body produces an antibody against a blood group antigen on its own red blood cells. (wikidoc.org)
  • Antigens, CD43" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uams.edu)
  • This graph shows the total number of publications written about "Antigens, CD43" by people in UAMS Profiles by year, and whether "Antigens, CD43" was a major or minor topic of these publications. (uams.edu)
  • Below are the most recent publications written about "Antigens, CD43" by people in Profiles over the past ten years. (uams.edu)
  • in comparison, a common mouse polymorph of SIRPalpha binds with similar affinity to human and mouse CD47 (K(d) approximately 0.22 microM). (nih.gov)
  • Only human phagocytes show significant differences in man versus mouse K(i)'s and only at CD47 levels below normal densities for RBCs. (nih.gov)
  • The Stanford group plans to start human clinical trials of the anti-CD47 cancer therapy in 2014. (stanford.edu)
  • CD47 expression is decreased on the surface of erythrocytes in obese subjects. (cusabio.com)
  • These changes in CD47 expression on the erythrocytes surface may be an adaptive response to hyperfibrinogenemia associated with obesity. (cusabio.com)
  • The XK appears to be required for proper synthesis or presentation of the Kell antigens on the red blood cell surface. (wikidoc.org)
  • Kell antigens are important in transfusion medicine , autoimmune hemolytic anemia and hemolytic disease of the newborn (anti-Kell) . (wikidoc.org)
  • 2007], we show here that inhibition of engulfment correlates with affinity of CD47 for SIRPalpha - but only at low levels of CD47. (nih.gov)
  • To bridge this gap, we developed an approach for enriching mIHC histology slides with single-cell RNA-seq data, building upon recent experimental procedures for augmenting single-cell transcriptomes with concurrent antigen measurements 7 , 8 . (biorxiv.org)
  • This is particularly true for the "K" antigen which shows a relatively high antigenicity and moderately low frequency (~9%) in Caucasian populations. (wikidoc.org)