• After leaving the bone marrow, the B cell acts as an antigen-presenting cell (APC) and internalizes offending antigens, which are taken up by the B cell through receptor-mediated endocytosis and processed. (wikipedia.org)
  • The LG.3A10 antibody reacts with CD27, a 45 - 55 kDa member of the tumor necrosis factor receptor superfamily. (stemcell.com)
  • Chimeric antigen receptor T (CART) cell therapy targeting the B cell specific differentiation antigen CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large B cell lymphoma (DLBCL) patients. (frontiersin.org)
  • CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. (thermofisher.com)
  • CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. (thermofisher.com)
  • By analysing iNKT cells from lymphoid tissues of mice and humans we demonstrate that their T cell receptor (TCR) repertoire is highly diverse and is distinct for cells from various tissues resulting in differential lipid-antigen recognition. (elifesciences.org)
  • Chimeric antigen receptor (CAR) T-cell therapy is an example of how immunotherapy is revolutionizing the treatment of hematologic malignancies with unprecedented response rates in patients with relapsed/refractory lymphoma, multiple myeloma, and acute lymphoblastic leukemia. (ajmc.com)
  • Chimeric antigen receptor (CAR) T-cell therapy is designed to enhance the body's immune system to effectively kill malignant cells. (ajmc.com)
  • Background Activation of the T-cell co-stimulatory receptor CD27 and blockade of the PD-1:PD-L1 axis can augment antitumor immune responses through distinct mechanisms. (bmj.com)
  • HexaBody ® -CD27 (GEN1053/BNT313) is a novel CD27 human monoclonal antibody with a functionally inert IgG1 Fc domain harboring a hexamerization-enhancing mutation to induce CD27 activation independently of Fc gamma receptor crosslinking, while also avoiding T-cell depletion. (bmj.com)
  • Transferred T cells that persist in the blood and form memory have a phenotype consistent with a less differentiated state (CD27/CD28/IL-7 receptor-α) [ 3 ]. (biomedcentral.com)
  • There is an unmet need to develop novel therapies for refractory/relapsed MM. In the past few years, chimeric antigen receptor (CAR)-modified T cell therapy for MM has shown promising efficacy in preclinical and clinical studies. (biomedcentral.com)
  • Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
  • CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. (bio-techne.com)
  • CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). (bio-techne.com)
  • Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
  • CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. (biolegend.com)
  • CD27 and its ligand CD70 are involved in multiple aspects of T cell biology, including the costimulation and expansion of CD4+ and CD8+ T cells and differentiation to memory CD8+ T cells. (stemcell.com)
  • In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. (thermofisher.com)
  • RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. (thermofisher.com)
  • CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). (bio-techne.com)
  • The second involves a network of co-inhibitory and co-stimulatory molecules pathways such as CD80/CD86-CD28/cytotoxic T lymphocyte antigen-4 (CTLA-4), inducible co-stimulator (ICOS)-ICOS ligand (ICOSL), programmed death-1 (PD-1), programme death ligand-1/2 (PD-L1/PD-L2), 4-1BB-4-1BB ligand (4-1BBL), CD40-CD154 ligand, OX40-OX40 ligand and CD27-CD70. (biomedcentral.com)
  • Linfócitos B foram fenotipados por marcadores CD19, CD27, CD70 e a produção intracelular de IgM por citometria de fluxo. (fiocruz.br)
  • B lymphocytes were phenotyped by CD19, CD27, CD70 markers and intracellular IgM production by flow cytometry. (fiocruz.br)
  • Detects mouse CD27 Ligand/TNFSF7 in direct ELISAs. (bio-techne.com)
  • Cell Proliferation Induced by CD27 Ligand/TNFSF7 and Neutralization by Mouse CD27 Ligand/TNFSF7 Antibody. (bio-techne.com)
  • In the presence of sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # MAB484 ), Recom-binant Mouse CD27 Ligand/ TNFSF7 (Catalog # 783-CL ) stimulates proliferation in mouse splenic T cells in a dose-dependent manner (orange line). (bio-techne.com)
  • Under these conditions, prolif-eration elicited by Recombinant Mouse CD27 Ligand/TNFSF7 (10 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse CD27 Ligand/ TNFSF7 Monoclonal Antibody (Catalog # MAB783). (bio-techne.com)
  • Measured by its ability to neutralize CD27 Ligand/TNFSF7-induced proliferation in mouse splenic T cells. (bio-techne.com)
  • The Neutralization Dose (ND 50 ) is typically 0.5-2 µg/mL in the presence of 10 µg/mL Recombinant Mouse CD27 Ligand/TNFSF7 and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody. (bio-techne.com)
  • These antibodies are transported from the plasma cells by the blood plasma and the lymphatic system to the site of the target antigen (foreign substance), where they initiate its neutralization or destruction. (wikipedia.org)
  • However, relapse of primary disease remains a major obstacle after CAR T cells therapy, and the majority of relapses present a tumor phenotype with retention of target antigen (antigen-positive relapse), which highly correlate with poor CAR T cells persistence. (biomedcentral.com)
  • Disease relapse following CAR T cell therapy can be categorized into two major patterns: target antigen loss relapse or antigen-positive relapse. (biomedcentral.com)
  • The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
  • CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. (biolegend.com)
  • Evaluation of Mycobacterium tuberculosis specific antigen-stimulated CD27 - CD38 + IFN-γ + CD4 + T cells for discrimination of active tuberculosis. (bvsalud.org)
  • HDAC9 is also expressed in marginal zone B cells and a fraction of CD38 or CD27 positive subepithelial tonsilar cells. (sunderland.ac.uk)
  • citation needed] Terminally differentiated plasma cells express relatively few surface antigens, and do not express common pan-B cell markers, such as CD19 and CD20. (wikipedia.org)
  • In vitro , CD3 + CD8 + CD27 - CD28 - compared to CD3 + CD8 + CD27 + CD28 + CART cells displayed similar CD19 + target cell-specific cytotoxicity, but were hypoproliferative and produced less cytotoxic cytokines (IFN-γ and TNF-α). (frontiersin.org)
  • Several antigens have been used as targets for CAR-T cell therapy against MM, including B cell maturation antigen (BCMA), CD19, CD138, signaling lymphocytic activation molecule 7 (SLAM7), and immunoglobulin light chains. (biomedcentral.com)
  • Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram). (biolegend.com)
  • A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
  • Human derived T lymphocytes engineered to express chimeric antigen receptors, which are expanded in vitro culture and then infused into patients exerting robust cytotoxicity after tumor antigen recognition and subsequent activation. (biomedcentral.com)
  • Full length human recombinant protein of human CD27 (NP_001233) produced in HEK293T cell. (thermofisher.com)
  • A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN . (nih.gov)
  • Here, we studied human pulmonary T-cell responses induced by the M. tuberculosis -specific antigen Rv3615c, a protein with a similar size and sequence homology to ESAT-6 and CFP-10, which induced dominant CD4 + T-cell responses in human tuberculosis (TB) models. (portlandpress.com)
  • At screening CSF concentrations, adjusted for age and sex, of NFL, myelin basic protein (MBP), soluble CD27, chitinase 3-like 1, and B-cell maturation antigen were higher than in a group of symptomatic controls. (ku.dk)
  • We analyzed 17DD-YF-specific memory by establishing the phenotypic features of peripheral blood mononuclear cells upon 17DD-YF antigen recall in vitro. (cdc.gov)
  • Methods The effects of combining HexaBody-CD27 with anti-PD-(L)1 antibodies (pembrolizumab, nivolumab, and atezolizumab) on human CD8 + T-cell proliferation and effector functions were investigated in vitro . (bmj.com)
  • CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. (biolegend.com)
  • Plasma cells, also called plasma B cells or effector B cells, are white blood cells that originate in the lymphoid organs as B cells and secrete large quantities of proteins called antibodies in response to being presented specific substances called antigens. (wikipedia.org)
  • naïve B cells are CD27−, memory B-cells are CD27+ and plasma cells are CD27++. (wikipedia.org)
  • This antigen is expressed at high levels on normal human plasma cells. (wikipedia.org)
  • Pieces of the antigen (which are now known as antigenic peptides) are loaded onto MHC II molecules, and presented on its extracellular surface to CD4+ T cells (sometimes called T helper cells). (wikipedia.org)
  • These T cells bind to the MHC II-antigen molecule and cause activation of the B cell. (wikipedia.org)
  • First, the B cells must encounter a foreign antigen and are then required to be activated by T helper cells before they differentiate into specific cells. (wikipedia.org)
  • They divide rapidly and are still capable of internalizing antigens and presenting them to T cells. (wikipedia.org)
  • Unlike their precursors, plasma cells cannot switch antibody classes, cannot act as antigen-presenting cells because they no longer display MHC-II, and do not take up antigen because they no longer display significant quantities of immunoglobulin on the cell surface. (wikipedia.org)
  • Differentiation through a T cell-independent antigen stimulation (stimulation of a B cell that does not require the involvement of a T cell) can happen anywhere in the body and results in short-lived cells that secrete IgM antibodies. (wikipedia.org)
  • CD27 is found on peripheral T cells, memory B cells, and NK cells and in mice has been found on most thymocytes, except CD46-CD8- precursors. (stemcell.com)
  • Additionally, CD27 plays important roles in T cell - B cell interactions and the maturation of effector T cells. (stemcell.com)
  • The expansion of activated B cells and their differentiation to plasma cells is also reported to be enhanced by CD27. (stemcell.com)
  • Univariate and multivariate regression analyses showed that low levels of differentiated CD3 + CD27 - CD28 - T cells (23.3 ± 19.3% versus 35.1 ± 18.0%) were independently associated with OR. (frontiersin.org)
  • Low frequency of differentiated CD3 + CD27 - CD28 - T cells at leukapheresis represents a novel pre-infusion blood biomarker predicting a favorable response to CART cell treatment in r/r DLBCL patients. (frontiersin.org)
  • CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. (thermofisher.com)
  • Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. (thermofisher.com)
  • Yet, if and how the tissue environment shapes the antigen specificity of iNKT cells remains unknown. (elifesciences.org)
  • This suggests that unique tissue-specific factors (including local lipid antigens, cytokines and/or hormones) may shape the population of iNKT cells resident in those tissues, ultimately regulating local immune responses. (elifesciences.org)
  • iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
  • central memory T cells/(CMCD4;CMCD8)/CD27+CD45RO+ and effector memory T cells/(EMCD4;EMCD8)/CD27-CD45RO+. (cdc.gov)
  • non-classical memory B cells/(nCMCD19)/CD27+IgD+ and classical memory B cells/(CMCD19)/CD27+IgD. (cdc.gov)
  • YF antigen recall of peripheral blood mononuclear cells. (cdc.gov)
  • In preclinical studies, HexaBody-CD27 enhanced proliferation and effector functions of activated T cells. (bmj.com)
  • Cytotoxic activity of claudin-6-specific human CD8 + T cells towards MDA-MB-231 tumor cells expressing cognate antigen was analyzed using real-time analysis of tumor cell mass, along with flow cytometric analysis of granzyme B and CD107a expression on CD8 + T cells. (bmj.com)
  • Results Combination treatment with HexaBody-CD27 and all tested anti-PD-(L)1 antibodies enhanced proliferation and proinflammatory cytokine secretion of antigen-specific CD8 + T cells in coculture with cognate antigen-expressing dendritic cells, compared to the respective single-agent treatments. (bmj.com)
  • Furthermore, the combination increased granzyme B and CD107a expression by antigen-specific CD8 + T-cells and enhanced CD8 + T-cell-mediated cytotoxic activity towards cognate antigen-expressing tumor cells. (bmj.com)
  • In MLR assays of human CD8 + T cells and allogeneic dendritic cells, the combination of HexaBody-CD27 and pembrolizumab synergistically enhanced IFN-γ secretion. (bmj.com)
  • 7. Klein U, Rajewsky K, Küppers R. Human immunoglobulin (Ig)M + IgD + peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells. (southernbiotech.com)
  • B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)
  • One is the ability to administer large numbers of highly selected cells with high avidity for tumor antigens that can recognize shared and patient-specific mutated (neo) antigens. (biomedcentral.com)
  • Here we demonstrate that certain HCV(+)MC(+) subjects have clonal expansions of immunoglobulin M (IgM)(+)kappa(+)IgD(low/-)CD21(low)CD27(+) B cells. (ox.ac.uk)
  • The finding that these hypermutated, marginal zone-like IgM(+)CD27(+) B cells are clonally expanded in certain subjects with MC offers insight into mechanisms of HCV-associated MC and B-cell malignancy. (ox.ac.uk)
  • As a side observeration the examined Hodgkin`s cells presented themselfs as CD27 negative. (uni-wuerzburg.de)
  • Here, we present a new generation of subunit vaccines targeting viral antigens to CD40-expressing antigen-presenting cells. (nature.com)
  • Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). (bio-techne.com)
  • Moreover, CD27(-)IgA(+) B cells could be a marker for treatment with TNF-α blockers. (eyehospital.nl)
  • Abstract T cells recognising cognate pMHC antigens become activated to elicit a myriad of cellular responses, such as target cell killing and the secretion of different cytokines, that collectively contribute to adaptive immunity. (ox.ac.uk)
  • When using primary human memory CD8 + T cells responding to autologous antigen presenting cells equivalent thresholds were also observed for cytokine production and killing. (ox.ac.uk)
  • Cells were cultured with different antigens (gp120 and p24 peptide, CpG and Lipopolysaccharide) for 24 hours to measure cytokines and IgM in the culture supernatant. (fiocruz.br)
  • Invariant natural killer T (iNKT) cells are CD1d-restricted T cells, which respond rapidly to antigen recognition and promote development of anti-tumor immunity in many tumor models. (biomedcentral.com)
  • 0.05), suggesting the selective elimination of DCs cross-presenting tumor-associated antigens by iNKT cells. (biomedcentral.com)
  • High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. (thermofisher.com)
  • Here, using systematic experiments in a reductionist system, where primary human CD8 + T cell blasts are stimulated by recombinant pMHC antigen alone, we show that different inflammatory cytokines have comparable antigen dose thresholds across a 25,000-fold variation in affinity. (ox.ac.uk)
  • CD27 interacts with TRAF family members, resulting in enhanced cellular proliferation and the induction of anti-apoptotic proteins. (stemcell.com)
  • CD8 + T-cell proliferation and cytokine secretion were assessed in an antigen-specific assay using claudin-6 as a model antigen. (bmj.com)
  • In the second part of this review, we provide evidence that frequent exercise enhances-rather than suppresses-immune competency, and highlight key findings from human vaccination studies which show heightened responses to bacterial and viral antigens following bouts of exercise. (frontiersin.org)
  • Instead of developing your own assays, explore our kits and services to quickly quantify viral antigens! (acrobiosystems.com)
  • The surface antigen CD138 (syndecan-1) is expressed at high levels. (wikipedia.org)
  • Another important surface antigen is CD319 (SLAMF7). (wikipedia.org)
  • These effector responses have been hypothesised to exhibit different antigen dose and affinity thresholds, suggesting that pathogen-specific information may be encoded within the nature of the antigen. (ox.ac.uk)
  • Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. (thermofisher.com)
  • A naive B cell is a mature B cell that has the phenotype surface IgD-positive, surface IgM-positive, CD20-positive, CD27-negative and that has not yet been activated by antigen in the periphery. (ontobee.org)
  • Despite this prototypical TCR repertoire gene usage, in recent years it has become apparent that there are variations within the iNKT cell repertoire that ultimately impact the antigen recognition capacity and consequently the functional outcomes during an immune response. (elifesciences.org)
  • These findings imply a simple phenotypic model of TCR signalling where multiple T cell responses share a common rate-limiting threshold and a conceptually simple model of antigen recognition, where the chance factor of antigen dose and affinity do not provide any additional response-specific information. (ox.ac.uk)
  • Multiparameter flow cytometric analysis of CD27 expression on Human peripheral blood leukocyte populations. (bdbiosciences.com)
  • However, continued exposure to antigen through those low levels of immunoglobulin is important, as it partly determines the cell's lifespan. (wikipedia.org)
  • As well as concerning the latency type of EBV, the expression of surface antigens and transcriptionfactors and the missing immunoglobulin synthesis Hodgkin`s disease and senile lymphoproliferation were equivalent to each other. (uni-wuerzburg.de)
  • Despite this, patients had normal serum immunoglobulin levels and normal antigen-specific immunoglobulin responses. (eyehospital.nl)
  • There is also a need for rational combinations beyond interleukin (IL)-2 and programmed death (PD)-1/ cytotoxic T-lymphocyte-associated antigen (CTLA)-4 immune checkpoint blockade and to combat exhaustion and restore costimulatory functions, e.g., with gene-engineered or pharmacologic agent-treated TILs. (biomedcentral.com)
  • Als Nebenbeobachtung stellten sich die hier untersuchten Hodgkin Zellen als CD27 negativ dar, die im Unterschied stehen, zur CD27 und CD30 positiven Memoryzelle, welche als korrespondierende Normalzelle postuliert wurde. (uni-wuerzburg.de)
  • This is a unexpected difference to the CD27 and CD30 positive memory cell which was postulated as the corresponding regular cell. (uni-wuerzburg.de)
  • This process favors, by selection for the ability to bind antigen with higher affinity, the activation and growth of B cell clones able to secrete antibodies of higher affinity for the antigen. (wikipedia.org)
  • This unique mechanism of action distinguishes HexaBody-CD27 from other CD27-targeting monoclonal antibodies. (bmj.com)
  • Although co-stimulation by CD28, CD2, and CD27 increased cytokine production in this system, the antigen threshold remained comparable across different cytokines. (ox.ac.uk)
  • Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD27 Antibody, Clone LG.3A10, followed by a goat anti-hamster (Armenian) IgG antibody, Alexa Fluor® 488 (filled histogram), or an Armenian hamster IgG isotype control antibody, followed by a goat anti-hamster (Armenian) IgG antibody, Alexa Fluor® 488 (solid line histogram). (stemcell.com)
  • The bivariate pseudocolor density plot showing the correlated expression of CD27 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. (bdbiosciences.com)
  • We characterized T-cell responses including cytokine profiling, kinetics of activation, expansion, differentiation, TCR usage, and signaling of activation induced by Rv3615c compared with other M. tuberculosis -specific antigens. (portlandpress.com)
  • Two main advantages of BCMA as an antigen for CAR-T therapy are the potential reduction of on-target/off-tumor toxicity and the lack of antigen-dependent reduction in CAR-T cell expansion [ 16 ]. (biomedcentral.com)
  • Carcinoembryonic antigen (CEA) is a glycoprotein involved in cell adhesion . (wikidoc.org)
  • As well as concerning the latency type of EBV, the expression of surface antigens and transcriptionfactors and the This work´s aime was to describe the EBV infected cell in infectious monunnocleosis, hodgkin`s disease, post-transplatation lymphoproliferations (PTLD) and senile lymphoproliferation. (uni-wuerzburg.de)
  • Therefore the CD27 negativity of the Hodgkin`s cell is not an EBV specific phenomenon and requires further investigation. (uni-wuerzburg.de)
  • Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node. (cuanschutz.edu)
  • HexaBody-CD27 is currently being evaluated in patients with advanced solid tumors ( NCT05435339 ). (bmj.com)
  • The following product was used in this experiment: CD27 Monoclonal Antibody (OTI6D6), TrueMAB™ from Thermo Fisher Scientific, catalog # TA812372. (thermofisher.com)
  • ScFv is the antigen-binding domain of CAR structure, which is composed of a single heavy and light chain of monoclonal antibody connected by a linker. (biomedcentral.com)