AntibodiesProteinEpstein-BarrHepatitis B virMinor capsidAntibodyRecombinantHisto-bloodVirus-like particlesDetectionVaccineNorovirusDiagnosticsProteinsAssaysRetroviralPolyproteinAntiviralCoatELISAStructuresVirusesHumanConcentrationReceptorFull-lengthInfectionCellsDataResultsDetailsModelSamplesAssemblyThought
Antibodies13
- It is the antigen targeted by antibodies that bond to viral capsid antigens (VCA). (wikipedia.org)
- EBV associated (WHO) International Laboratory and Collaborating Cen- antibodies, such as virus capsid antigen (VCA-IgA) and ters for Biological Standards. (sagepub.com)
- This kit is used to qualitatively detect EBV capsid antigen IgM antibodies in human vein whole blood or serum (plasma) samples in vitro. (bjzjat.com)
- Chimeric rabbit/human Fab antibodies against the hepatitis Be-antigen and their potential applications in assays, characterization, and therapy. (nih.gov)
- People are considered susceptible to EBV infection if they do not have antibodies to the VCA (viral capsid antigen). (medicinenet.com)
- People are considered to have a primary EBV infection if they have anti-VCA IgM but do not have antibodies to EBNA (EBV nuclear antigen). (medicinenet.com)
- These patients have modestly elevated antibodies to EBV lytic antigens as well as antibodies to the EBV nuclear antigens (EBNAs), including EBNA1. (frontiersin.org)
- Antibodies to viral capsid antigen (VCA) are often used to study the relationship of EBV with chronic diseases. (cdc.gov)
- Indirect immunofluorescence assays (IFAs) using two epitope-specific monoclonal antibodies (MAbs) were used to examine the expression kinetics of two potentially immunogenic and diagnostically relevant viral antigens, an envelope glycoprotein and a capsid-associated protein. (stir.ac.uk)
- ELISA was used to test for anti-HPV antibodies using virus-like particles composed of capsid antigen L1 produced in a baculovirus expressing system in insect cells. (bmj.com)
- Pre-treatment samples were analyzed for IgG antibodies against antigens on cytomegalovirus (CMV) and Epstein Barr virus (EBV), early nuclear antigen-1 (EBNA-1), viral capsid antigen (VCA), early antigen-diffuse (EA-D), and also for vitamin D status (25 hydroxy-vitamin D3 level), smoking/nicotine exposure (cotinine level), and HLA DRB1*1501 status. (hcplive.com)
- A panel of antibodies against envelope (ENV) and capsid (GAG) antigens was tested. (bvsalud.org)
- The presence or absence of EBV viral capsid antigen (VCA) IgG, VCA IgM and EBV nuclear antigen (EBNA) IgG antibodies was recorded. (who.int)
Protein18
- The Epstein-Barr virus viral-capsid antigen (EBV-VCA) is the viral protein that forms the viral capsid of the Epstein-Barr virus. (wikipedia.org)
- Here we report the cryo-electron microscopy structure of a tubular HIV-1 capsid-protein assembly at 8 Å resolution and the three-dimensional structure of a native HIV-1 core by cryo-electron tomography. (nature.com)
- Cryo-EM structural data have been deposited in the EMDataBank under accession codesEMD-5582 andEMD-5639, and the MDFF atomic model of the CA HOH and models of HIV-1 capsid have been deposited in the Protein Data Bank under accession numbers 3J34 , 3J3Q , 3J3Y . (nature.com)
- Structure of the amino-terminal core domain of the HIV-1 capsid protein. (nature.com)
- Crystal structure of dimeric HIV-1 capsid protein. (nature.com)
- Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding. (nature.com)
- The deduced amino acid sequences of the outer capsid protein, VP7, of serotype G1 rotavirus clinical isolates collected over a 6 year period (1990-1995) in Melbourne, Australia, were examined. (microbiologyresearch.org)
- This property of CW3 is dependent on its major capsid protein, a virulence determinant. (broadinstitute.org)
- The assay, which utilizes a truncated 56 kDa recombinant HEV capsid protein expressed in insect cells as antigen, was performed as described previously (1), with modifications (2). (cdc.gov)
- Instead of detecting a single specific protein associated with the invader, they detect repetitive molecules linked together, such as those found in a bacterial cell wall or viral capsid. (nationaljewish.org)
- Norwalk virus is a small virus that consists of a single strand of RNA , which comprises the genetic material of the virus, surrounded by multiple copies of a single protein assembled into a protective coat that is called the capsid. (bcm.edu)
- The different colors represent different regions of the capsid protein. (bcm.edu)
- An 8-tenfold increase in capsid-associated protein expression was observed during the first 5days post-infection compared to a ≤2-fold increase in glycoprotein expression. (stir.ac.uk)
- A dominant protein of ~100kDa reacted with the capsid-associated MAb (20F10) in western blot analysis. (stir.ac.uk)
- This band was also recognised by sera obtained from carp infected with CyHV-3, indicating that this capsid-associated protein is produced in abundance during infection in vitro and is immunogenic to carp. (stir.ac.uk)
- Description: Astrovirus Capsid protein (60 kDa), recombinant protein from E. coli, 1.06 mg/mL. (chipready.com)
- LOCUS AB665364 7542 bp RNA linear VRL 27-SEP-2016 DEFINITION Norovirus Hu/GII.4/Osaka/NV2210/2007/JP genes for nonstructural polyprotein, capsid protein, minor capsid protein, partial and complete cds. (cdc.gov)
- The single RNA is complexed with a capsid protein. (medscape.com)
Epstein-Barr4
- Keywords: Epstein-Barr virus, Nasopharyngeal carcinoma, Virus capsid antigen, Reference, Enzyme-linked immunosorbent assay (ELISA) Introduction of discussion concerns the potential use of nature refer- Nasopharyngeal carcinoma (NPC) is highly endemic in ence preparations to calibrate these assays for quantita- South China. (sagepub.com)
- Most commercial ELISA Epstein-Barr virus nuclear antigen 1 (EBNA1-IgA), are kits generate only negative or positive results [16]. (sagepub.com)
- Description: Epstein-Barr Virus (Strain P3HR1) gp125 VCA, inactivated antigen. (cropsciencejournal.org)
- High Epstein-Barr virus capsid antigen IgG level associates with the carriership of CD8+ T cell somatic mutations in the STAT3 SH2 domain. (cdc.gov)
Hepatitis B vir3
- Capsids of hepatitis B virus e antigen with authentic C termini are stabilized by electrostatic interactions. (nih.gov)
- Structures of Hepatitis B Virus Core- and e-Antigen Immune Complexes Suggest Multi-point Inhibition. (nih.gov)
- Zhang, Y Discovery of hepatitis B virus capsid assembly inhibitors leading to a heteroaryldihydropyrimidine based clinical candidate (GLS4). (bindingdb.org)
Minor capsid1
- Dr. Roden's team is currently engaged in research to prevent cervical cancer through the development of a minor capsid antigen L2-based vaccine that is active against all oncogenic types of human papillomavirus (HPV). (hopkinsmedicine.org)
Antibody2
- They found that a molecule that binds toll-like receptors (TLR) doubles the early antibody response to an antigen, and shifts it to a more effective, IgG form. (nationaljewish.org)
- Upon illumination, antigen-antibody binding produces a detectable change in reflectance, the inventors claim. (genomeweb.com)
Recombinant1
- The Recombinant Norovirus Group-2 Capsid P-Domain, E.Coli derived, VA387 Strain contains a.a. from 225-520 having a Mw of 30kDa. (prospecbio.com)
Histo-blood2
- Moreover, P domain has a receptor binding region which recognizes human histo-blood group antigens (HBGAs). (prospecbio.com)
- P-domain has a receptor binding region to recognize human histo-blood group antigens (HBGAs). (prospecbio.com)
Virus-like particles1
- The chip consists of a multilayer substrate that hosts virus-like particles or viral-capsid fragments from different viral strains or isolates that are covalently attached at different locations. (genomeweb.com)
Detection3
- Chen, Shulin 2020-04-29 00:00:00 Background: The detection of the Epstein-Barr capsid antigen (VCA) immunoglobulin A (IgA) is widely used in the diagnosis of nasopharyngeal carcinoma (NPC), but a reference standard for evaluating the presence of VCA-IgA is not yet available. (sagepub.com)
- The team is also focused on identifying novel tumor antigens of significance in the biology of ovarian cancer that are applicable as biomarkers for early detection or targets for immunotherapy. (hopkinsmedicine.org)
- All nine laboratories performing NS1 antigen detection obtained the correct results. (who.int)
Vaccine1
- The HPV vaccine is composed of antigen known as a virus-like particle (VLP) and adjuvant. (koreaherald.com)
Norovirus2
- Murine Norovirus Infection Induces T1 Inflammatory Responses to Dietary Antigens. (broadinstitute.org)
- Here we show that the acute CW3 strain of murine norovirus, but not the persistent CR6 strain, induces T1 immunity to dietary antigen. (broadinstitute.org)
Diagnostics1
- This abundantly expressed and immunogenic capsid-associated antigen may be a useful candidate for KHV serological diagnostics. (stir.ac.uk)
Proteins3
- The group antigens form the viral core structure and are the major proteins which comprise the nucleoprotein core particles. (ukessays.com)
- VLPs are composed of HPV L1 capsid (coat) proteins and resemble the actual virus morphologically but cannot induce infection as these do not contain viral DNA. (koreaherald.com)
- A physiological expression of GAG proteins in neuronal cells was observed in normal brain, whereas there was a striking accumulation of GAG antigen in axonal structures in demyelinated white matter from patients with MS. Prominent HERV-W GAG expression was also detected in endothelial cells of MS lesions from acute or actively demyelinating cases, a pattern not found in any control. (bvsalud.org)
Assays1
- In the transplant population, antigen assays or PCR is used (sometimes in conjunction with cytopathology) for diagnosis and treatment determinations. (medscape.com)
Retroviral1
- The retroviral promoter can direct high-level, efficient expression of genes encoded within the viral capsid of its genome using chromatin. (ukessays.com)
Polyprotein1
- Gag is a polyprotein and is an acronym for Group Antigens (ag). (ukessays.com)
Antiviral1
- AB-836, our oral capsid inhibitor, demonstrated robust antiviral activity, however, two patients in the 200 mg cohort experienced alanine aminotransferase (ALT) elevations. (biospace.com)
Coat1
- Nanorobots may extend chemosensory pads ( Section 4.2.8 ) which will selectively and reversibly adhere to the capsid coat of a nonenveloped viral target. (nanomedicine.com)
ELISA2
- Antigen Elisa Laboratories manufactures the astrovirus antigen in faeces kit elisa reagents distributed by Genprice. (chipready.com)
- The Astrovirus Antigen In Faeces Kit Elisa reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (chipready.com)
Structures1
- The cryo-electron-microscopy structures enable modelling by large-scale molecular dynamics simulation, resulting in all-atom models for the hexamer-of-hexamer and pentamer-of-hexamer elements as well as for the entire capsid. (nature.com)
Viruses3
- Intestinal reovirus infection can trigger T helper 1 (T1) immunity to dietary antigen, raising the question of whether other viruses can have a similar impact. (broadinstitute.org)
- For enveloped viruses, the nanorobot must carefully pick over the camouflage lipid membrane to find viral-specific antigens. (nanomedicine.com)
- Adenoviruses are DNA viruses classified according to 3 major capsid antigens (hexon, penton, and fiber). (msdmanuals.com)
Human1
- Antigen expression of a human endogenous retrovirus family, HERV-W, in normal human brain and multiple sclerosis lesions was studied by immunohistochemistry by three independent groups. (bvsalud.org)
Concentration1
- The antigen concentration approximated 0.025g per well. (cdc.gov)
Receptor1
- So the researchers decided to inject mice with the synthetic antigen plus a molecule that binds an innate receptor, known as TLR ligand. (nationaljewish.org)
Full-length3
- The full length of VP1 capsid comprises the internal N-terminal, Hinge, shell (S) and protruding (P) domains. (prospecbio.com)
- P particle displays an increased binding activity to HBGAs higher than virus-like particle (VLP) formed by the full-length capsid. (prospecbio.com)
- VLP) formed by the full length capsid. (prospecbio.com)
Infection1
- The rate of expression of these antigens was determined following a time-course of infection in two CyHV-3 susceptible cell lines. (stir.ac.uk)
Cells1
- Most CD8 cells are directed to lytic antigens initially, and these cells rapidly undergo apoptosis ( 7 ). (frontiersin.org)
Data1
- These data show that viral capacity to trigger specific inflammatory pathways at sites where T cell responses to dietary antigens take place interferes with the development of tolerance to an oral antigen. (broadinstitute.org)
Results1
- However, the quantitative results of some kits are still inconsistent due to the diversity of VCA antigens. (sagepub.com)
Details1
- X-ray structure of the Norwalk virus capsid, with the inset showing details of the structure of the subunits. (bcm.edu)
Model3
- The complete atomic HIV-1 capsid model provides a platform for further studies of capsid function and for targeted pharmacological intervention. (nature.com)
- Figure 4: All-atom HIV-1 capsid model. (nature.com)
- This process has been studied for many years using synthetic molecules as model antigens. (nationaljewish.org)
Samples3
- This supplemental testing included re-testing of samples using the EIA system described above as well as conducting an anti-HEV antigen- specific blocking assay. (cdc.gov)
- For the blocking assay, each sample and controls were pre-incubated with either PBS or HEV antigen (Sar55) at 1.0ug/ml for approximately 18 hours at 25°C. Next, the samples were tested by the standard anti-HEV EIA procedure described above. (cdc.gov)
- PBS- treated versus HEV antigen-treated OD values were compared and the samples that showed 40% or more blocking were classified as reactive and specific. (cdc.gov)
Assembly2
- however, structural information regarding the assembled capsid and the contacts between the assembly units is incomplete. (nature.com)
- Mutagenesis studies confirm that hydrophobic residues in the centre of the three-helix bundle are crucial for capsid assembly and stability, and for viral infectivity. (nature.com)
Thought1
- Drs. Torres Swanson thought that experiments using just the synthetic antigens did not accurately reflect what occurs in the real world. (nationaljewish.org)