• Formalin-fixed paraffin sections of normal mouse spleen after antigen retrieval were reacted with the anti-CD44 antibody. (bdbiosciences.com)
  • The IM7 antibody specific for mouse CD44 is recommended to test for immunohistochemical staining of formalin-fixed paraffin and acetone-fixed frozen sections. (bdbiosciences.com)
  • The IM7 antibody specifically recognizes an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). (bdbiosciences.com)
  • Antibody Elisa Laboratories manufactures the elisa test for ebola antibody and antigen reagents distributed by Genprice. (mytaq.net)
  • The Elisa Test For Ebola Antibody And Antigen reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (mytaq.net)
  • 1983) used a murine monoclonal antibody (A3D8) to identify an erythrocyte antigen inhibited by the In(Lu) gene. (wdv.com)
  • Phospho-CD44 (Ser706) Antibody detects endogenous levels of CD44 only when phosphorylated at Serine 706. (affbiotech.cn)
  • This antibody was obtained by immunization of CD44 recombinant protein (Accession Number: NM_001001389). (novoprolabs.com)
  • 1X10^6 HeLa cells were stained with 0.2ug CD44 antibody (Catalog No:109124, red) and control antibody (blue). (novoprolabs.com)
  • Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:109124(CD44 Antibody) at dilution of 1:200 (under 10x lens). (novoprolabs.com)
  • Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:109124(CD44 Antibody) at dilution of 1:200 (under 40x lens). (novoprolabs.com)
  • 2 ml monoclonal antibody against Rat CD44, R-PE Conjugated. (celltechgen.com)
  • First, the cells are labeled by Rat CD44 monoclonal antibody conjugated to R-PE and anti-R-PE magnetic beads, the Rat CD44 + cells are positively selected on a magnetic column, are collected for further flow-cytometric analysis. (celltechgen.com)
  • The screening procedure is performed by directly magnetically labeling the cells with the monoclonal antibody against the specific antigen, conjugated to R-PE and Anti-R-PE magnetic beads, then the labeled cells are subsequently retained to a magnetic column, and the target cells are released and collected for further flow cytometric analysis. (celltechgen.com)
  • ProductData": { "ID": "RTD000659", "ProductType": "Others", "BrandName": "anti-CD44 (SP37) Rabbit Monoclonal Primary Antibody", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use. (roche.com)
  • Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "Anti-CD44 (SP37) Rabbit Monoclonal Primary Antibody contains sufficient reagent for 50 tests. (roche.com)
  • One 5 mL dispenser of anti-CD44 (SP37) contains approximately 3.5 μg of a rabbit monoclonal antibody. (roche.com)
  • Anti-CD44 (SP37) is a recombinant monoclonal antibody produced from supernatant material. (roche.com)
  • Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "L'anticorps Anti-CD44 (SP37) Rabbit Monoclonal Primary Antibody est dirigé contre la protéine CD44. (roche.com)
  • Cet anticorps est conçu pour être utilisé en diagnostic in vitro (DIV)", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "Anti-CD44 (SP37) is a rabbit monoclonal antibody produced against a conserved region of the CD44 protein. (roche.com)
  • Immunotherapies with antibody-drug-conjugates (ADC) and CAR-T cells, targeted at tumor surface antigens (surfaceome), currently revolutionize clinical oncology. (lu.se)
  • CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. (biolegend.com)
  • CD44 is a transmembranous glycoprotein (80 kDa) responsible for cell adhesion that is expressed in a wide range of normal tissues, such as tonsil, breast, prostate, skin, bladder and cervical squamous epithelium (1). (biocare.net)
  • CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. (wdv.com)
  • 1989) demonstrated that the lymphocyte homing receptor is identical to the human leukocyte surface glycoprotein called CDw44, on the basis of studies at the Third International Workshop on Human Leukocyte Differentiation Antigens. (wdv.com)
  • 1989)' 'demonstrated' 'sameness' 'the human leukocyte surface glycoprotein' 'is called' 'CDw44' 'studies' 'were conducted at' 'the Third International Workshop on Human Leukocyte Differentiation Antigens. (wdv.com)
  • CD44 is a cell-surface glycoprotein that plays a role in cell-cell interactions, cell adhesion and migration. (whispermovie.com)
  • CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. (novoprolabs.com)
  • CD44 is a transmembrane molecule appearing in numerous isoforms generated by insertions of alternatively spliced variant exons ( CD44v ) and having various binding partners. (nature.com)
  • CD44 is a transmembrane molecule with multiple, in part poorly understood functions. (nature.com)
  • CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. (biolegend.com)
  • Expression of the adhesion molecule CD54 (ICAM-1) and the antigen presenting cell costimulatory molecule CD40 were also significantly elevated in both hapten and prohapten (+ S9) treated cells, however S9 alone also upregulated CD54. (cdc.gov)
  • CVO and DMBA, but not BaP, induced inconsistent increases in the adhesion molecule CD44. (cdc.gov)
  • Several antigens have been used as targets for CAR-T cell therapy against MM, including B cell maturation antigen (BCMA), CD19, CD138, signaling lymphocytic activation molecule 7 (SLAM7), and immunoglobulin light chains. (biomedcentral.com)
  • Since leucocyte-endothelial cell interactions involve various adhesion receptors we have analysed the expression and distribution pattern of the neural cell adhesion molecule (NCAM), the intercellular adhesion molecule-1 (ICAM-1), the vascular cell adhesion molecule-1 (VCAM-1), the endothelial leucocyte adhesion molecule-1 (ELAM-1), and the cluster of differentiation antigen-44 (CD44) on flat preparations of normal and organ-cultured HCECs. (bmj.com)
  • 5 The CD44 molecule is known to present several different glycosylation, oligomerization, and protein sequence patterns due to differential mRNA splicing. (roche.com)
  • Methods: Expression of postulated stem cell markers aldehyde dehydrogenase (ALDH), CD44, and CD133 was analyzed in UM cell lines and primary UM short-term cultures (STCs) established from tumor samples. (whiterose.ac.uk)
  • T RM responses against tumor/self-antigens can concurrently result in the development of pathogenic T RM responses to self, with a growing number of autoimmune diseases and inflammatory pathologies being attributed to T RM responses. (frontiersin.org)
  • While innate immune cells are important for early tumor immune surveillance, T cells are fundamentally recognized for their crucial role in the antigen-specific recognition and elimination of malignantly transformed cells ( 2 ). (frontiersin.org)
  • Two main advantages of BCMA as an antigen for CAR-T therapy are the potential reduction of on-target/off-tumor toxicity and the lack of antigen-dependent reduction in CAR-T cell expansion [ 16 ]. (biomedcentral.com)
  • The latter is a tool previously developed by Jacopo, 2020, able to identify tumor antigens similar to pathogen antigens in order to exploit molecular mimicry and tumor pathogen cross-reactive T cells in cancer vaccine development. (elifesciences.org)
  • These peptides were then tested in our previously described oncolytic cancer vaccine platform PeptiCRAd, a vaccine platform that combines an immunogenic oncolytic adenovirus (OAd) coated with tumor antigen peptides. (elifesciences.org)
  • CD44 participates in a wide variety of cellular functions such as lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. (whispermovie.com)
  • The cancer diagnostics market is on the verge of explosion, as the researchers approach major technological breakthroughs in tumor diagnosis and therapy, discover new specific antigens, and unlock the mystery of the genetic basis of the disease. (aarkstore.com)
  • 3. Endo K., Terada T. Protein expression of CD44 (standard and variant isoforms) in hepatocellular carcinoma: relationships with tumor grade, clinicopathologic parameters, p53 expression, and patient survival. (roche.com)
  • Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44 17,18 that is located between amino acids 145 and 186 20 . (biolegend.com)
  • However, numerous further isoforms of CD44 are generated by insertions of different variable regions into a single site of its extracellular domain. (nature.com)
  • In contrast to CD44s, the CD44 isoforms containing variable regions are expressed only in distinct epithelial cell types (e.g., keratinocytes), activated immune cells, and several types of cancers. (nature.com)
  • CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. (bdbiosciences.com)
  • CD44 antigen, the main cell surface receptor for HA. (wikipedia.org)
  • There is an unmet need to develop novel therapies for refractory/relapsed MM. In the past few years, chimeric antigen receptor (CAR)-modified T cell therapy for MM has shown promising efficacy in preclinical and clinical studies. (biomedcentral.com)
  • Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
  • Androgen induced expression of androgen receptor (AR), and co-culture with rat urogenital sinus further induced expression of prostate-specific antigen, a hallmark of secretory cells, suggesting that E-PZ-iPS-like cells have the capacity to differentiate into prostatic basal and secretory epithelial cells. (nih.gov)
  • 1990) demonstrated that CD44 is the main cell surface receptor for hyaluronate. (wdv.com)
  • CD44 is a receptor for hyaluronic acid and also interacts with other ligands, such as osteopontin, collagens, and matrix metalloproteinases. (whispermovie.com)
  • Although B- and T-cell development were normal, CD4(+)T cells lacking Emk exhibited a marked upregulation of the memory marker CD44/pgp-1 and produced more gamma interferon and interleukin-4 on stimulation through the T-cell receptor in vitro. (jcvi.org)
  • 1. Aruffo A, Stamenkovic I, et al.CD44 is the principal cell surface receptor for hyaluronate. (roche.com)
  • Expression of CD44s, the most common CD44 isoform, has been reported to be associated with increased survival of patients with nodenegative invasive breast carcinoma (3). (biocare.net)
  • CD44 antigen isoform 1 precursor. (biossusa.com)
  • Here we identified a T VM cell-originated CD44 super-high(s-hi) CD49d lo CD8 + T cell subset with features of tissue residency. (nature.com)
  • Mechanistically, CD44 s-hi CD49d lo CD8 + T cells could be induced from conventional T VM cells by interleukin (IL)-12, IL-15 and IL-18 stimulation. (nature.com)
  • Pathogenic activity of CD44 s-hi CD49d lo CD8 + T cells was mediated by NKG2D-dependent innate-like cytotoxicity, which was further augmented by IL-15 stimulation and triggered disease onset. (nature.com)
  • Fig. 1: CD44 s-hi CD49d lo CD8 + T cell population causes alopecia areata. (nature.com)
  • Fig. 2: CD44 s-hi CD49d lo CD8 + T cells originate from T VM cells. (nature.com)
  • Fig. 3: CD44 s-hi CD49d lo CD8 + T cells are derived from T VM cells by cytokine stimulation. (nature.com)
  • Fig. 4: CD44 s-hi CD49d lo CD8 + T cells have strong effector functions with T RM cell features. (nature.com)
  • Fig. 5: Cytolytic activity of CD44 s-hi CD49d lo CD8 + T cells is abrogated by NKG2D blockade. (nature.com)
  • CD44 and CD49d are putative activity markers and CD44 a potential novel therapeutic target in multiple sclerosis. (whispermovie.com)
  • Here we demonstrate that Cd44v7 -deficient T cells - like Cd44 wild-type ( Cd44 WT ) T cells - provoked disease in two different colitis models: the model induced by CD4 + CD45RB high T-cell transfer into Rag2 -deficient mice and a new model based on ovalbumin (OVA)-specific T-cell transfer into Rag -sufficient, OVA-challenged mice. (nature.com)
  • Importantly, stimulation of T cells by Cd44v7- deficient macrophages induced upregulation of Foxp3 in vitro , while cotransfer of Cd44 WT macrophages into Cd44v7- deficient mice reduced Foxp3 + T-cell counts and caused colitis. (nature.com)
  • As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. (biolegend.com)
  • High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. (biolegend.com)
  • Virtual memory T (T VM ) cells are a T cell subtype with a memory phenotype but no prior exposure to foreign antigen. (nature.com)
  • The antigen-specific CD8 + T cell repertoire in unimmunized mice includes memory phenotype cells bearing markers of homeostatic expansion. (nature.com)
  • White, J. T., Cross, E. W. & Kedl, R. M. Antigen-inexperienced memory CD8 + T cells: where they come from and why we need them. (nature.com)
  • CD8α + dendritic cell trans presentation of IL-15 to naive CD8 + T cells produces antigen-inexperienced T cells in the periphery with memory phenotype and function. (nature.com)
  • Results: We demonstrate that expression of ALDH, CD44, and CD133 does not select for a subpopulation of stem-like cells in either UM cell lines or UM STCs. (whiterose.ac.uk)
  • Immunohistochemical staining of CD44 positive cells. (bdbiosciences.com)
  • CD44 positive cells can be identified by the brown labeling of their cell membranes. (bdbiosciences.com)
  • The standard form of CD44, lacking variable exons and referred to as CD44H or CD44s, is widely expressed on hematopoietic and non-hematopoietic cells. (bdbiosciences.com)
  • It acts by targeting cells expressing CD44 antigen. (pharmaceutical-technology.com)
  • In the early 2000's, large and persistent populations of antigen (Ag)-specific CD8 T cells in peripheral tissues were initially classified as T EM cells in recirculation from the blood ( 8 , 11 ). (frontiersin.org)
  • Upregulation of activation and costimulatory markers in antigen presenting cells are key events in the sensitization process and have been reported to serve as indicators of skin sensitization. (cdc.gov)
  • The present study evaluated the effect of hapten and prohapten exposure on cell surface marker expression (CD86, CD54, CD44 and CD40) on THP-1 cells. (cdc.gov)
  • Stimulation of naive CD4 cells by specific antigen results in proliferation and changes in cell surface marker expression as the cells differentiate into effector and memory cells. (unboundmedicine.com)
  • Several of the marker changes (e.g., differences in CD45RB, CD44, and L-selectin levels) appear to be relatively stable and permit the identification of memory T cells. (unboundmedicine.com)
  • CD4(+) T cells from DO11.10 TCR transgenic mice were labeled with the fluorescent dye carboxyfluorescein diacetate succinimidyl ester (CFSE) and were stimulated with specific antigen (OVA323-339). (unboundmedicine.com)
  • AU - Lee,W T, AU - Pelletier,W J, PY - 1998/9/23/pubmed PY - 1998/9/23/medline PY - 1998/9/23/entrez SP - 1 EP - 11 JF - Cellular immunology JO - Cell Immunol VL - 188 IS - 1 N2 - Stimulation of naive CD4 cells by specific antigen results in proliferation and changes in cell surface marker expression as the cells differentiate into effector and memory cells. (unboundmedicine.com)
  • Indeed, antigen-presenting cells are important reservoirs of HIV-1 [ 16,17 ] and induction of HIV-1 replication within these cells may contribute significantly to the cofactor effect of confections on HIV-1 pathogenesis. (lww.com)
  • Rapamycin enhanced expansion of peripheral antigen-specific CD8 T cells and IFNγ production following ex vivo antigen stimulation. (aacrjournals.org)
  • Manufacturers are attempting to develop CAR T-cells that are engineered to hit other antigens, including CD20 and CD44. (ahdbonline.com)
  • However flat mounts of HCECs from organ-culture preserved corneas showed a mosaic-like distribution pattern of VCAM-1 and ELAM-1 positive cells and garland-like clusters of CD44 positive cells. (bmj.com)
  • Importantly, E-PZ-iPS-like cells re-expressed basal epithelial cell markers (CD44, p63, MAO-A) in response to prostate-specific medium in spheroid culture. (nih.gov)
  • Finally, when injected into mice, E-PZ-iPS-like cells expressed basal epithelial cell markers including CD44 and p63. (nih.gov)
  • When co-injected with rat urogenital mesenchyme, E-PZ-iPS-like cells expressed AR and expression of p63 and CD44 was repressed. (nih.gov)
  • Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. (biomedcentral.com)
  • Finally, mononuclear cells isolated from lung (L-MCs), which are used as precursors for L-DCs, expressed more antigen-presenting cell-associated markers such as MHC class II and CD172α compared to their counterparts from blood. (biomedcentral.com)
  • Dendritic cells (DCs) are the most important antigen-presenting cells (APCs) in the body. (biomedcentral.com)
  • This kit is developed to isolate and detect the Rat CD44 + cells using magnetically labeled with Magnetic beads. (celltechgen.com)
  • The detection kit is developed to isolate and detect the target cells with the specific antigen. (celltechgen.com)
  • CD44 is expressed in many normal human hematopoietic, epithelial,and mesenchymal cells. (roche.com)
  • Therefore, early in the formation of oral lichen planus lesions, CD8 + T cells may recognize an antigen associated with the major histocompatibility complex (MHC) class I on keratinocytes. (medscape.com)
  • After antigen recognition and activation, CD8 + cytotoxic T cells may trigger keratinocyte apoptosis. (medscape.com)
  • Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
  • Description: This is Ebola virus NP recombinant antigen for ELISA,WB. (mytaq.net)
  • Overall, B-DCs demonstrated higher expression of CD86 and CD172α than L-DCs, but both cell types expressed high levels of MHC class II and CD44, as well as moderate amounts of CD163, CD204, and Bla36. (biomedcentral.com)
  • They act as a surveillance system to detect foreign antigens and shape immunogenic or tolerogenic responses [ 1 ]. (biomedcentral.com)
  • 1,2 The extensive glycosylation patterns of CD44 are important for the recognition and binding of the CD44-hyaluronan complex. (roche.com)
  • 1,2,3,4 CD44 is one of two major hyaluronan (HA) binding proteins required for activation of hyaluronan. (roche.com)
  • 5. Bartolazzi A., Nocks A., et al.Glycosylation of CD44 is implicated in CD44-mediated cell adhesion to hyaluronan. (roche.com)
  • In breast cancer studies, evaluation of CD44 expression has been useful in the differentiation of benign and malignant papillary lesions (2). (biocare.net)
  • We aimed to determine the significance of detecting expression of some antigens that might be pivotal to the process, namely CD44 and CD117, in patients with active pemphigus vulgaris. (who.int)
  • The immunohistochemical results showed prominent expression of CD44 in 13 of the patients and CD117 in 9 of the patients with new blister formation. (who.int)
  • We suggest that modulation of ELAM-1, VCAM-1, and CD44 expression on HCECs may contribute to the regulation of leucocytes-HCECs interaction in the case of anterior segment inflammation. (bmj.com)
  • Liu D, Sun J, Zhu J, Zhou H, Zhang X, Zhang Y. Expression and clinical significance of colorectal cancer stem cell marker EpCAM(high)/CD44(+) in colorectal cancer. (novoprolabs.com)
  • 4. Southgate J., Trejdosiewicz L. et al.Patterns of splice variant CD44 expression by normal human urothelium in situ and in vitro and by bladder-carcinoma cell lines. (roche.com)
  • The expression or unmasking of the lichen planus antigen may be induced by drugs (lichenoid drug reaction), contact allergens in dental restorative materials or toothpastes (contact hypersensitivity reaction), mechanical trauma (Koebner phenomenon), viral infection, or other unidentified agents. (medscape.com)
  • 13 Furthermore, OPN promotes T-helper type 1 (Th1) responses during chronic intestinal inflammation in mice and is significantly increased in the serum of patients with IBD, suggesting that the CD44-OPN system is deregulated in these patients. (nature.com)
  • T cell memory is antigen-specific, and can provide durable host-wide protection. (frontiersin.org)
  • However, these MM-specific antigens have yet to be identified. (biomedcentral.com)
  • Similar to RBC Blood Group antigen the Human Platelet Antigen (HPAs) are each defined by antigen specific alloantibody sera. (bloodantigens.com)
  • The specific antigen that triggers lichen planus is unknown, although it may be a self-peptide (or altered self-peptide), in which case lichen planus would be a true autoimmune disease. (medscape.com)
  • Studies have shown that CD44, also known as HCAM, plays an important role in the cascade of metastasis and progression of human malignant tumors (2). (biocare.net)
  • Korinek V, Stefanova I, Angelisova P, Hilgert I, Horejsi V: The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1. (exbio.cz)
  • It is likely that L-DCs play an important role in antigen uptake and processing of respiratory pathogens and are major contributors to respiratory tract immunity and may be ideal tools for future in vitro or ex vivo studies. (biomedcentral.com)
  • In addition, B-cell responses to T-cell-dependent and -independent antigen challenge were altered in vivo. (jcvi.org)
  • More recent studies suggested that DCs derived from tissues without "danger" signal stimulation should be regarded as immature DCs, based on their major role in antigen uptake and endocytosis of antigens [ 11 , 14 ]. (biomedcentral.com)
  • Resultados: as células isoladas mostraram alta expressão para CD44 (98,8%), CD73 (100%) e CD90 (97,2%) e uma expressão moderada para STRO-1 (18,4%) e ALDH1 (16,2%), por meio da citometria de fluxo. (bvsalud.org)
  • Han S, Guo J, Liu Y. Knock out CD44 in reprogrammed liver cancer cell C3A increases CSCs stemness and promotes differentiation. (novoprolabs.com)
  • However, unlike the RBC Blood Groups Systems, the HPA Systems were not named on a per gene or related gene basis with one or more antigens in each system. (bloodantigens.com)
  • Rather, one HPA gene can contain multiple HPA systems so in reality each biallelic antithetical antigen pair is really its own system. (bloodantigens.com)
  • For example, the HPA-1 system contains the antithetical antigens HPA-1a and HPA-1b, but this same gene also contains the HPA-4 system (HPA-4a and HPA-4b). (bloodantigens.com)
  • Concurrent with this observation, L-MCs possessed significantly more antigen-uptake capacity compared to their counterparts from blood. (biomedcentral.com)
  • Correlation of ALDH1, CD44, OCT4 and SOX2 in tongue squamous cell carcinoma and their association with disease progression and prognosis. (novoprolabs.com)