Compared with the polyclonal and monoclonal antibodies of MME, this MME recombinant antibody has the features of increased reproducibility and control, animal-free technology, high degree of monovalency, high batch-to-batch consistency, easier isotype conversion, etc. (cusabio.com)
In the 2000s rituximab a chimeric monoclonal antibody against the protein CD20 which is definitely primarily found on the surface of B lymphocytes was launched for the management of ABO-IKT [3 4 This protocol has become standard in multiple centers after Tydén et al. (techuniq.com)
Epitope mapping and specificity of the anti-alpha-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines. (qmul.ac.uk)
Cinaciguat (BAY 58-2667) is the first of a new class of soluble guanylate cyclase (sGC) activators in clinical development for acute decompensated heart failure. (biochempartner.com)
One retinal antigen implicated in the autoimmune mechanism of CAR is recoverin, a 23 kDa photoreceptor-specific calcium-binding protein modulating the activity of photoreceptor guanylyl cyclase. (univmed.org)
Other circulating antibodies directed against a 46 kDa protein identified as retinol enolase and a 60 kDa retinal protein have been demonstrated in patients with clinically diagnosed CAR syndrome. (univmed.org)
Depending on which family the G protein is, it goes on to activate (G αs protein subunit) or inhibit (G αi protein subunit) the membrane-bound cyclase. (pancreapedia.org)
Three-train L-LTP was also blocked by inhibitors of guanylyl cyclase or cGMP-dependent protein kinase (PKG). (jneurosci.org)
These results suggest that NO contributes to L-LTP by stimulating guanylyl cyclase and cGMP-dependent protein kinase, which acts in parallel with PKA to increase phosphorylation of the transcription factor CREB. (jneurosci.org)
Both NPR1 and NPR2 contain five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. (antibodypedia.com)
The protein is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. (antibodypedia.com)
As written above, the G i -protein inhibits adenylyl cyclase (to the left on the figure), and the G s -protein activates adenylyl cyclase (to the right on the figure). (greek.doctor)
Note that norepinephrine and epinephrine bind to β1 and β2-receptors, which activates G s which activates adenylyl cyclase, but acetylcholine and adenosine bind to muscarinic type 2 and adenosine type 1 receptors, which activates G i protein which inhibits adenylyl cyclase. (greek.doctor)
Class of soluble1
Two recent reports, however, show that there is likely to be an additional class of soluble guanylyl cyclases that do not need a second subunit for activity. (biologists.com)
Adenylyl1
Failure of relaxation is less likely with multiple transmitters than with only one, and the potential for more efficacious relaxation is enhanced, with these transmitters activating both the adenylyl cyclase-cyclic AMP and guanylyl cyclase-cyclic GMP pathways. (ersjournals.com)
Phosphodiesterase3
In these photoreceptors, the C-terminus of the rhodopsin is directly linked to either a histidine kinase (histidine kinase rhodopsin), a phosphodiesterase (rhodopsin phosphodiesterase, RPDE), or a guanylyl cyclase (rhodopsin guanylyl cyclase, RGC). (elifesciences.org)
Here we show that in platelets and aortic tissue, NO led to a biphasic response characterized by a tremendous increase in cGMP (up to 100-fold) in less than 30 s and a rapid decline, reflecting the tightly controlled balance of guanylyl cyclase and phosphodiesterase activities. (rupress.org)
Phosphodiesterase 2 inhibition preferentially promotes NO/guanylyl cyclase/cGMP signaling to reverse the development of heart failure. (qmul.ac.uk)
Guanylate cyclases1
BAY 41-2272 is a soluble guanylate cyclases (sGC) activator. (biochempartner.com)
Receptor2
Guanylate cyclase C (GUCY2C, commonly known as GC-C, or STa receptor, STaR) is an enzyme encoded by the GUCY2C gene in humans and belongs to the receptor guanylate cyclase family. (adcreviews.com)
Conditional ablation of NFIX from neural stem or progenitor cells, or neuroblasts causes migration defects due to aberrant neuroblast branching, partly resulted from an increased expression of the guanylyl cyclase natriuretic peptide receptor 2 (Npr2). (anggonolab.org)
Conjugate1
HDP-101, an Antibody Targeted Amanitin Conjugate directed against the antigen BCMA, is being tested in a Phase I/IIa open-label, multicenter study for the treatment of relapsed or refractory multiple myeloma, a cancer of the bone marrow. (heidelberg-pharma.com)
Enzyme4
Guanylate cyclase soluble subunit alpha-2 is an enzyme that in humans is encoded by the GUCY1A2 gene. (wikipedia.org)
MsGC-β3, identified in the tobacco hornworm Manduca sexta , was the first example of a soluble guanylyl cyclase that exhibited enzyme activity without the need for coexpression with additional subunits. (biologists.com)
A cyclase enzyme (lyase) catalyzes the formation of the cyclic nucleotide from its nucleotide triphosphate precursor ( Figure 1 ). (pancreapedia.org)
The usage of immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) serves as a valuable tool for the diagnosis of acute flaviviral infections, since IgM antibody titers are detectable early, peak at about 2 weeks postinfection, and subsequently decline to lower levels over the next few months. (techuniq.com)
20011
2013 Yu and Watts 2013 though many limitations have also surfaced including evidence that high-affinity TfR antibodies remain trapped within mind vasculature (Moos and Morgan 2001 Gosk et al. (biotech-angels.com)
Heme-bindin1
In the cytosol, the signal molecule binds to the heme-binding domain of the soluble cyclase. (pancreapedia.org)
Small signalin1
The second signaling mechanism involves the binding of a small signaling molecule to a soluble cyclase. (pancreapedia.org)
Phosphodiesterases2
Enzymerhodopsins represent a recently discovered class of rhodopsins which includes histidine kinase rhodopsin, rhodopsin phosphodiesterases, and rhodopsin guanylyl cyclases (RGCs). (elifesciences.org)
Most of the effects of the signaling molecule nitric oxide (NO) are mediated by cGMP, which is synthesized by soluble guanylyl cyclase and degraded by phosphodiesterases. (rupress.org)
CGMP1
Plecanatide is an agonist of guanylate cyclase-C. Plecanatide increases intestinal transit and fluid through a buildup of cGMP. (biochempartner.com)
Catalytic1
The relaxation is reduced by prior incubation with VIP antiserum 2 , by a VIP antagonist, α-chymotrypsin 3 or a VIP catalytic antibody. (ersjournals.com)
Subunit2
Molecular cloning and expression of a new alpha-subunit of soluble guanylyl cyclase. (wikipedia.org)
Soluble guanylyl cyclases are typically obligate heterodimers, composed of a single alpha and a single beta subunit. (biologists.com)
Conjugates1
Indeed delivery of drug-Tf conjugates and TfR antibody conjugates have had some success (Dufès AMG-925 et al. (biotech-angels.com)
Mice2
The IHC tests showed that the ECM of the mice testicular interstitial tissue showed reaction to anti-laminin-α5 antibody. (biomedres.info)
Malaria-specific antibody responses and parasite persistence after infection of mice with Plasmodium chabaudi chabaudi. (mdc-berlin.de)
Antigen1
5] 1st launched antigen-specific immunoabsorption combined with anti-CD20 antibody and Sonnenday et al. (techuniq.com)
Promotes1
Two aspartic acid residues in the active site of the cyclase (AC or GC) promotes the binding of ATP. (pancreapedia.org)
Activity1
Similar to NO, CO stimulates soluble guanylyl cyclase activity. (ersjournals.com)
Immunoprecipitation1
For immunoprecipitation experiments, HA-tag or Myc-tag antibody-conjugated agarose beads had been purchased from Cellular Signaling Technology. (healthweblognews.info)
Parallel1
A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. (rndsystems.com)
Function1
2011 We also lately reported that affinity and effector function determine the basic safety profile of TfR healing antibodies in vivo hence further helping low-affinity strategies and the necessity to better understand the root cell biology (Sofa et al. (biotech-angels.com)
Cell3
live\cell imaging or by immunofluorescence using an antibody focusing on the candidate. (informationalwebs.com)
GCC19CART is a CAR-T cell therapy based on ICT's unique technology platform, CoupledCAR®, which targets guanylate cyclase C (GUCY2C) in the treatment of refractory metastatic colorectal cancer (R/R mCRC) [1]. (adcreviews.com)
Unlike their biological counterparts (principally antibodies and siRNA), synthetic small molecules may be cell-permeable, easily delivered at different doses with accurate quantitation and selectable timing, and their specificity can be modified simply. (biomedcentral.com)
Specific2
In certain patients no specific antibody has been identified. (univmed.org)
Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
Brain2
2013 Right here we hypothesized that TfR antibody affinity determines TfR trafficking destiny and sought to review the cellular systems root the robust distinctions between high and low anti-TfR affinity variations and TfR trafficking aswell as the influence of the strategies on human brain uptake of biotherapeutics. (biotech-angels.com)
Outcomes High-affinity binding to TfR drives cortical TfR degradation in vivo To comprehend how anti-TfR affinity inversely AMG-925 influences human brain contact with antibody we initial determined whether degrees of TfR are influenced by dosing of high- versus low-affinity TfR bispecific antibodies. (biotech-angels.com)
Cells1
Antibodies to recoverin induced apoptosis of photoreceptor and bipolar cells in vivo. (univmed.org)
Technology1
The phospho Raptor Ser877 antibody (09C107) was from Millipore, and every one of the various other antibodies were attained from Cellular Signaling Technology. (healthweblognews.info)