DihydrofolateMonoclonal antibodyMethylenetetrahydrofolate ReductaseTYMS1.45PolyclonalChromosomalTyrosineProteinMutationsELISAReceptorHumanMarkersCell linesExpressionStructureProductionStudiesFamilyDihydrofolate reductaseMonoclonal antibodiesTumor cellsRecombinantPolyclonal antibodyInhibitorsPolymorphismsBindsQuantitationPeptideGeneThymidineCellsAntigenABSTRACTEGFRHumanEnzymeCatalogInteractionCellLevelsLevelStatusTreatmentPatientsTime
Dihydrofolate2
- Pregnant women (n = 418) were screened for malaria during routine antenatal care by using two RDTs that detect histidine-rich protein 2 (HRP2) or Plasmodium lactate dehydrogenase, and enzyme-linked immunosorbent assays with antibodies that detect dihydrofolate reductase-thymidylate synthase or heme-detoxification protein, and compared with real-time polymerase chain reaction (RT-PCR) and microscopy for evaluation of their diagnostic accuracy. (ined.fr)
- The RT-PCR and the HRP2 RDT detected most cases of malaria during pregnancy, whereas microscopy, the Plasmodium lactate dehydrogenase RDT, and enzyme-linked immunosorbent assays for dihydrofolate reductase-thymidylate synthase and heme-detoxification protein antibodies did not detect several low-density infections. (ined.fr)
Monoclonal antibody2
- evaluation of a monoclonal antibody typing system for herpes simplex virus. (liverpool.ac.uk)
- Tecentriq (atezolizumab) is a monoclonal antibody against PD-L1 (CD274), preventing activation of its receptor, potentially enhancing T-cell-mediated immune response to neoplasms and inhibiting T-cell inactivation ( PMID: 29449897 ). (jax.org)
Methylenetetrahydrofolate Reductase2
- Genetic Variation of Methylenetetrahydrofolate Reductase (MTHFR) and Thymidylate Synthase (TS) Genes Is Associated with Idiopathic Recurrent Implantation Failure. (bookriff.com)
- Journal Article] Polymorphisms in thymidylate synthase and methylenetetrahydrofolate reductase genes and the susceptibility to esophageal and stomach cancer with smoking and drinking habits. (nii.ac.jp)
TYMS1
- The Visual Perception Pathway complements our catalog of research reagents including antibodies and ELISA kits against TNFSF14, TYMS, PYCARD, IGKV1-5, PMEL. (novusbio.com)
1.451
- Specificity: This antibody recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). (scytek.com)
Polyclonal1
- TS and p53 status were determined, blinded to any clinical data, by immunohistochemistry using a validated polyclonal antibody or the DO-7 clone, respectively, and their relationships with overall survival were examined. (ox.ac.uk)
Chromosomal1
- These possess included intratumoral degrees of thymidylate synthase (TS), TP53 mutations, microsatellite instability, and chromosomal deletions [6-8]. (careersfromscience.org)
Tyrosine2
- The present study evaluated the prognostic value of the epidermal growth factor receptor (EGFR) mutation status, and excision repair cross-complementation group 1 (ERCC1) and thymidylate synthase (TS) expression following intercalated tyrosine kinase inhibitor (TKI) therapy and platinum- and pemetrexed-based chemotherapies (subsequent second-line treatment) for patients with adenocarcinoma non-small-cell lung cancer (AC-NSCLC). (medsci.org)
- Therapies tested include traditional medical chemotherapeutic agents and new generation targeted therapies, such as monoclonal antibodies and tyrosine kinase inhibitors, hormone blocking therapies, and an array of over forty nutrient, biological and herbal therapies. (dunphynunley.com)
Protein1
- Figure-1: HEK293 cell line transfected with irrelevant protein (B) and human CD138 (A) were surface stained with anti-CD138 neutralizing antibody 1μg/ml (indatuximab ravtansine) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. (abeomics.com)
Mutations1
- As p53 mutations are found in 50-60% of colon cancers, chemotherapy agents such as CPT-11 should be used in combination with anti-Fas antibodies. (gutscharity.org.uk)
ELISA2
- We have 5636 products for the study of Chagas Disease that can be applied to Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits. (novusbio.com)
- an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. (liverpool.ac.uk)
Receptor5
- If we combine these drugs with an antibody which activates the receptor we increase significantly the number of cancer cells killed by the drugs. (gutscharity.org.uk)
- The ability of many drugs to increase expression of the Fas death receptor in colon cancer cell lines raises the possibility of using an antibody similar to the one used in our lab to activate the receptor and cause increased cancer cell death when used in conjunction with conventional chemotherapy agents. (gutscharity.org.uk)
- Secondly, the ability of one drug (CPT-11) to induce the receptor in p53 mutant cells may mean that in these patients it should be used in preference to the other chemotherapy drugs to enable the antibody to have the desired effect. (gutscharity.org.uk)
- The ability of antibodies which bind to the Fas receptor in colon cancer cells to enhance cancer cell kill raises the possibility of using these antibodies in clinical practice to improve patient care. (gutscharity.org.uk)
- In addition, the data presented in this research indicate that certain chemotherapy agents are more efficacious in activating the Fas death receptor and priming the cells to respond to the antibody in p53 mutant cells. (gutscharity.org.uk)
Human3
- Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site. (umassmed.edu)
- This study aimed to identify single-chain variable fragment (scFvs) antibodies from synthetic antibody phage libraries, which can recognize either of the three SSLs and could block the interaction between the SSLs and their respective human targets. (bvsalud.org)
- limiting dilution analysis of specific in vitro anti-herpes simplex virus antibody production by human lymphocytes. (liverpool.ac.uk)
Markers2
- A prospective, blinded analysis of thymidylate synthase and p53 expression as prognostic markers in the adjuvant treatment of colorectal cancer. (ox.ac.uk)
- BACKGROUND: Despite previous studies, uncertainty has persisted about the role of thymidylate synthase (TS) and p53 status as markers of prognosis in colorectal cancer (CRC). (ox.ac.uk)
Cell lines1
- It is used to select hybridoma cell lines in production of monoclonal antibodies. (wikipedia.org)
Expression1
- Several biomarkers have been used to predict treatment responses, such as the EGFR mutation status for targeted therapy or the excision repair cross-complementation group 1 (ERCC1) and thymidylate synthase (TS) expression for conventional chemotherapy [ 3 - 8 ]. (medsci.org)
Structure1
- Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization. (umassmed.edu)
Production1
- hsv specific antibody production was detected in culture supernatants of pbmc from hsv seropositive healthy individuals by stimulating with optimal concentrations of hsv antigen without mitogens. (liverpool.ac.uk)
Studies1
- Initial studies of such antibodies were hampered by excess liver toxicity with the first antibody tested, but studies are ongoing to develop safer alternatives. (gutscharity.org.uk)
Family1
- Two different classes of thymidine kinases have been identified and are included in this super family: one family groups together thymidine kinase from herpesvirus as well as cellular thymidylate kinases, the second family groups TK from various sources that include, vertebrates, bacteria, the bacteriophage T4, poxviruses, African swine fever virus (ASFV) and Fish lymphocystis disease virus (FLDV). (wikipedia.org)
Dihydrofolate reductase1
- It specifically inhibits thymidylate synthase (TS), dihydrofolate reductase (DHFR), and glycinamide ribonucleotide formyltransferase (GARFT), which are folate-dependent enzymes involved in de novo biosynthesis of thymidine and purine nucleotides. (medscape.com)
Monoclonal antibodies2
- It is used to select hybridoma cell lines in production of monoclonal antibodies. (wikipedia.org)
- Standards have recently been established in the adjuvant treatment of patients with gastrointestinal carcinoma, and promising new drugs, including monoclonal antibodies, thymidylate synthase, and topoisomerase inhibitors, as well as taxoids, are currently being tested in phase I, II, and III trials of gastrointestinal tumors. (ernst-dietrich-kreuser.de)
Tumor cells1
- rTS expression during growth and in response to thymidylate synthase inhibitors in human tumor cells. (nih.gov)
Recombinant3
- Ipilimumab is a targeted a recombinant, human cytotoxic T-lymphocyte antigen 4 (CTLA-4)-blocking antibody. (medscape.com)
- This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-85895. (novusbio.com)
- Specific antibodies against recombinant protein were prepared for ASP/RON1 and RAMA, since they are predicted to have C-terminal glycosylphosphatidylinositol (GPI) anchored domains and therefore the modification of their C-terminal structure might disrupt their function. (pberghei.eu)
Polyclonal antibody1
- TS and p53 status were determined, blinded to any clinical data, by immunohistochemistry using a validated polyclonal antibody or the DO-7 clone, respectively, and their relationships with overall survival were examined. (ox.ac.uk)
Inhibitors1
- 8. Thymidylate synthase level as the main predictive parameter for sensitivity to 5-fluorouracil, but not for folate-based thymidylate synthase inhibitors, in 13 nonselected colon cancer cell lines. (nih.gov)
Polymorphisms1
- We studied the polymorphisms in thymidylate synthase, epidermal growth factor receptor, glutathione S-transferase pi 1 (GSTP1), and several DNA repair genes to evaluate their usefulness as pharmacogenetic markers in a cohort of 128 rectal cancer patients treated with preoperative chemoradiotherapy. (nih.gov)
Binds3
- This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- HN - 2016 MH - Adalimumab UI - D000068879 MN - D12.776.124.486.485.114.224.60.250 MN - D12.776.124.790.651.114.224.60.250 MN - D12.776.377.715.548.114.224.200.250 MS - A humanized monoclonal antibody that binds specifically to TNF-ALPHA and blocks its interaction with endogenous TNF RECEPTORS to modulate INFLAMMATION. (nih.gov)
- Dacetuzumab specifically binds to and inhibits the CD40 receptor, thereby inducing apoptosis and inhibiting cellular proliferation via antibody-dependent cellular cytotoxicity (ADCC) in cells that overexpress this receptor. (blogspot.com)
Quantitation2
- 3. Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines. (nih.gov)
- The quantitation by radioimmunoassay of 2'-deoxyuridine 5'-triphosphate in extracts of thymidylate synthase-inhibited cells. (ox.ac.uk)
Peptide1
- 19. 5-fluorouracil-based chemotherapy enhances the antitumor activity of a thymidylate synthase-directed polyepitopic peptide vaccine. (nih.gov)
Gene1
- This locus was originally identified as the source of antisense RNAs of the adjacent thymidylate synthase gene. (nih.gov)
Thymidine1
- Two different classes of thymidine kinases have been identified and are included in this super family: one family groups together thymidine kinase from herpesvirus as well as cellular thymidylate kinases, the second family groups TK from various sources that include, vertebrates, bacteria, the bacteriophage T4, poxviruses, African swine fever virus (ASFV) and Fish lymphocystis disease virus (FLDV). (wikipedia.org)
Cells7
- 7. Regulation of thymidylate synthase in human colon cancer cells treated with 5-fluorouracil and interferon-gamma. (nih.gov)
- 9. Gossypol sensitizes the antitumor activity of 5-FU through down-regulation of thymidylate synthase in human colon carcinoma cells. (nih.gov)
- 10. Down-regulation of mitochondrial F1F0-ATP synthase in human colon cancer cells with induced 5-fluorouracil resistance. (nih.gov)
- 11. Arsenic trioxide suppresses thymidylate synthase in 5-FU-resistant colorectal cancer cell line HT29 In Vitro re-sensitizing cells to 5-FU. (nih.gov)
- 17. Differentiation-inducing factor-1 enhances 5-fluorouracil action on oral cancer cells inhibiting E2F1 and thymidylate synthase mRNAs accumulation. (nih.gov)
- The assay has been used to assess the presence of dUTP in A549 human lung carcinoma cells exposed to the thymidylate synthase inhibitor CB3717. (ox.ac.uk)
- Replication of NiV Delta C was followed by the production of NiV-specific antibodies and associated with higher recruitment of inflammatory cells and less intensive histopathological lesions in different organs than in wild-type-NiV-infected animals. (nartsignaling.com)
Antigen1
- This product is intended to be used as a blocking antigen for antibody competition assays. (novusbio.com)
ABSTRACT1
- ABSTRACT: Thymidylate synthase (TS) is a tumor-associated enzyme critical for DNA replication and main 5′-fluorouracil (5′-FU) target. (unipa.it)
EGFR1
- EGFR mono-antibody salvage therapy for locally advanced and distant metastatic penile cancer: Clinical outcomes and genetic analysis. (cdc.gov)
Human2
- 4. Induction of thymidylate synthase associated with multidrug resistance in human breast and colon cancer cell lines. (nih.gov)
- Understanding the structural basis of species selective, stereospecific inhibition for Cryptosporidium and human thymidylate synthase. (cornell.edu)
Enzyme1
- Thymidylate synthase (TS), found in all organisms, is an essential enzyme responsible for the de novo synthesis of deoxythymidine monophosphate. (cornell.edu)
Catalog1
- The following product was used in this experiment: E2F1 Monoclonal Antibody (KH95) from Thermo Fisher Scientific, catalog # 32-1400, RRID AB_2533065. (thermofisher.com)
Interaction1
- Kinetics and thermodynamics of the interaction of 5-fluoro-2'-deoxyuridylate with thymidylate synthase. (colorado.edu)
Cell1
- Antiparietal cell antibodies are present in 90% of PA cases. (medscape.com)
Levels2
- Abnormally low vitamin B-12 levels: Test for PA by measuring antibodies against IF. (medscape.com)
- In patients older than 70 years, 10% have false-positive abnormal antibody levels. (medscape.com)
Level1
- If antibodies are negative, obtain a serum gastrin level to test for achlorhydria, which is associated with PA. (medscape.com)
Status1
- BACKGROUND: Despite previous studies, uncertainty has persisted about the role of thymidylate synthase (TS) and p53 status as markers of prognosis in colorectal cancer (CRC). (ox.ac.uk)
Treatment1
- Tanya Lehky, "Anti-Tac (1L2-alpha) antibody treatment for HTLV-1-associated myelopathy. (nih.gov)
Patients2
- IF antibodies are present in 60% of patients. (medscape.com)
- Schilling test: The Schilling test is used to determine the etiology of vitamin B-12 deficiency in patients with normal IF antibodies. (medscape.com)