• A chimeric virus, designated MN-III-2, was selected using anti-V3 loop monoclonal antibodies. (usda.gov)
  • Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
  • Despite their efficiency, adoptive T-cell therapies show unique toxicities, which are distinct from those seen with conventional chemotherapies, monoclonal antibodies (mAbs), and small-molecule-targeted therapies. (nature.com)
  • Antigenic characterization using a panel of serotype G1-specific neutralizing monoclonal antibodies classified lineage II isolates (1990-1993) as monotype G1a while lineage I isolates were classified as monotype G1b (1993-1995). (microbiologyresearch.org)
  • Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. (microbiologyresearch.org)
  • Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping of human rotaviruses. (microbiologyresearch.org)
  • Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein Appl Microbiol Biotechnol. (absoluteantibody.com)
  • The original paper describes the generation of six monoclonal antibodies specific for UL42 protein of PRV. (absoluteantibody.com)
  • Conversely, broad spectrum therapeutics like drugs and monoclonal antibodies can be generated targeting these key distant regions of the spike protein. (biorxiv.org)
  • Monoclonal antibodies to the FLAG epitope served to monitor cell surface expression for functional analysis of the BFIV21 class I glycoprotein. (usda.gov)
  • AMPA receptor variants were identified with a polyclonal antibody recognizing the conserved extracellular loop region of all four AMPA receptor subunits (GluR1-4, both flip and flop ), whereas NMDA receptors were immunolabeled with a polyclonal antibody that binds to an extracellular N-terminal epitope of the NR1 subunit, common to all splice variants. (jneurosci.org)
  • This antiserum gave a similar immunofluorescence pattern in T. brucei to those of an epitope-tagged ribosomal protein, QM. (gla.ac.uk)
  • A potent class of HIV-1 broadly neutralizing antibodies (bnAbs) targets the envelope glycoprotein's membrane proximal exposed region (MPER) through a proposed mechanism where hypervariable loops embed into lipid bilayers and engage headgroup moieties alongside the epitope. (elifesciences.org)
  • We sought to address this phenomenon for broadly neutralizing antibodies (bnAbs) 4E10, PGZL1, 10E8, and LN01 of unique lineages which all target the semi-concealed membrane-proximal epitope region (MPER) 13 - 16 of the HIV-1 envelope glycoprotein (Env). (elifesciences.org)
  • Indeed, modified residues close to one PTM may prevent the PTM-specific antibody to bind its epitope. (eurogentec.com)
  • To address these pitfalls, we propose dynamic Multi-channel Equivariant grAph Network (dyMEAN), an end-to-end full-atom model for E(3)-equivariant antibody design given the epitope and the incomplete sequence of the antibody. (arxiv.org)
  • Specifically, we first explore structural initialization as a knowledgeable guess of the antibody structure and then propose shadow paratope to bridge the epitope-antibody connections. (arxiv.org)
  • Finally, the updated antibody is docked to the epitope via the alignment of the shadow paratope. (arxiv.org)
  • The antibody recognises an epitope including residues 342-367 (sequence RMYTPIAK). (absoluteantibody.com)
  • BF residues 78 and 81 (HLA 79 and 82) form an antibody epitope with a slight effect on ALV antigen presentation, consistent with their predicted orientation based on the HLA-A2 crystal structure. (usda.gov)
  • Antibodies that recognize extracellular epitopes and can thus label iGluRs on living neurons are important tools for the study of receptor localization and dynamics. (jneurosci.org)
  • Our data confirm that VOC, particularly those such as B.1.351 with substitutions at residues 484 and 417, escape neutralization by antibodies directed to the ACE2-binding Class 1 and the adjacent Class 2 epitopes but are susceptible to neutralization by the generally less potent antibodies directed to Class 3 and 4 epitopes on the flanks of the RBD. (nature.com)
  • The work provides insights into how broadly neutralizing antibodies associate with lipids proximal to membrane-associated epitopes to drive neutralization. (elifesciences.org)
  • Antibodies can target epitopes on integral membrane proteins very near to the lipid bilayer surface, even those partially embedded within the headgroup region. (elifesciences.org)
  • A better understanding of how antibodies develop membrane affinity and target membrane-proximal epitopes would be impactful for antibody therapeutics, auto-immunity, and vaccine development 10 - 12 . (elifesciences.org)
  • Functional analysis employing site directed mutagenesis identified BF amino acid residues forming serologic epitopes as well as residues important in antigen presentation to ALV induced cytotoxic T lymphocytes. (usda.gov)
  • As little as one amino acid substitution in the HA1 can result in an antigenic drift of influenza viruses, indicating the dominance of some epitopes in the binding of HA to polyclonal serum antibodies. (cdc.gov)
  • In this study, we have developed a biolayer interferometry (BLI)-based assay to determine dominant binding epitopes of the HA1 in antibody response to influenza vaccines using a panel of recombinant HA1 proteins of A(H1N1)pdm09 virus with each carrying a single amino acid substitution. (cdc.gov)
  • L104M, L115Q, V210A) between the HA of the virus from the Chilean case and A/Astrakhan/3212/2020-like candidate vaccine, and they are not in major antigenic epitopes strongly suggesting that antibodies elicited by the A/Astrakhan/3212/2020-like vaccine would be expected to have good cross-reactivity - and therefore protection - against this virus. (cdc.gov)
  • Antibodies recognize specific configurations (epitopes, or antigenic determinants) on the surfaces of antigens (eg, proteins, polysaccharides, nucleic acids). (msdmanuals.com)
  • The same antibody molecule can cross-react with related antigens if their epitopes are similar enough to those of the original antigen. (msdmanuals.com)
  • Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716). (rndsystems.com)
  • A PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109 ). (rndsystems.com)
  • In the process of engaging the antigen, antibodies complementarity-determining regions (CDR) may need to navigate interactions with or concealment by lipid molecules. (elifesciences.org)
  • In addition, the Federal Circuit rejected the "newly characterized antigen" test as a means of satisfying the written description requirement for a claim to an antibody. (klgates.com)
  • if you find that the level of skill and knowledge in the art of antibodies at the time of filing was such that production of antibodies against such an antigen was conventional or routine. (klgates.com)
  • That test allowed the jury to find that a claim to an antibody had written description support based on disclosure of the structure, formula, chemical name, or physical properties of a newly characterized antigen, so long as production of antibodies against such an antigen was conventional or routine. (klgates.com)
  • In contrast, a site directed substitution in residue 34 dramatically alters ALV antigen presentation by the BFIV21 class I glycoprotein. (usda.gov)
  • Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)
  • Structure of antibody-bound peptides and retro-inverso analogues. (rcsb.org)
  • With regard to the NH amide region, one set was similar to that for RI(mG) while the second was similar to those for the parent L-peptides L(A) and L(G). The antibody-bound conformations of the two couples of L- and retro-inverso peptides have been analyzed using molecular modeling calculations based on the transferred NOE interproton distances. (rcsb.org)
  • A polyclonal antibody targeting the dimethyl-Lysine 9 of Histone 3 was tested against various H3 peptides and shown to bind to all peptides containing the dimethyl-Lysine 9, no matter the surrounding residues. (eurogentec.com)
  • The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. (thermofisher.com)
  • Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
  • They are usually small peptides 10-30 residues in length with positively charged amino acid sequences. (lifetein.com)
  • Residue 34 is located in pocket B and contacts the main chain carbon of bound peptides in HLA-A2. (usda.gov)
  • Supporting Information: LC-MS characterization of GSH-MDI reaction products, Tables of peptides matched to albumin or modified albumin, and MS/MS data on the GSH-MDI modified albumin peptide containing residues 411-428. (cdc.gov)
  • The PA1-083 immunogen is a synthetic peptide corresponding to residues C R(1043) Q A D E E K H K R Q M S V P G S(1058) of human CRM1. (thermofisher.com)
  • Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. (caslab.com)
  • After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. (cellsignal.com)
  • The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (genetex.com)
  • After showing that antibodies are gamma globulins, he used oligosaccharides of different lengths to interfere with antibodies attempting to bind to the blood plasma substitute dextran, accurately estimating the size and shape of antibody-binding sites before the development of X-ray crystallography. (wikipedia.org)
  • Previously we have shown that these chimeric HRV14:HIV viruses can induce animals to make and can also bind antibodies that stop HIV from infecting cells in the laboratory. (usda.gov)
  • 1] Notably, Amgen's patents do not claim any particular antibody by amino acid sequence, but instead claim the entire genus of antibodies that bind to at least one of 15 specified amino acid residues of PCSK9 and block PCSK9 from binding to LDL-R. The specifications disclose a trial-and-error process "used to generate and screen antibodies that bind to PCSK9 and block PCSK9 from binding to LDL-Rs. (klgates.com)
  • 3] Sanofi argued that Amgen's patents did not disclose enough species of antibodies to provide written description support for claims to the entire genus of antibodies that bind to PCSK9 at the specified amino acid residues. (klgates.com)
  • Despite sequence identities ranging from 87% to 91% between caprine and bovine caseins, these patients possess IgE antibodies that bind specifically to the caprine caseins without recognizing the bovine ones. (cea.fr)
  • The CRIB/PBD protein motif has been shown to bind specifically to the GTP-bound form of Rac and/or Cdc42 proteins. (cytoskeleton.com)
  • The globular head domain of influenza virus surface protein hemagglutinin (HA1) is the major target of neutralizing antibodies elicited by vaccines. (cdc.gov)
  • The HA is also the primary target of neutralizing antibodies elicited by infection or vaccination, and the HA of virus from this specimen is very closely related (99% identity) to the A/Astrakhan/3212/2020-like pre-pandemic candidate vaccine viruses (e.g. (cdc.gov)
  • Spectrin, the major component of the membrane skeleton that provides elasticity to the cell, contains tandemly repeated sequences of 106 amino acid residues. (embl.de)
  • Spectrin, the major component of the erythroid membrane skeleton, is a long, asymmetrical rodlike protein that interacts with several other proteins to form a two-dimensional membrane skeleton. (embl.de)
  • Its product, podocin, is a new member of the stomatin family, which consists of hairpin-like integral membrane proteins with intracellular NH2- and COOH-termini. (jci.org)
  • Many eukaryotic cell surface proteins are attached to the plasma membrane by glycosylphosphatidylinositol (GPI) anchors. (gla.ac.uk)
  • Simulations estimating protein-membrane interaction strength for PGZL1 variants along an inferred maturation pathway show bilayer affinity is evolved and correlates with neutralization potency. (elifesciences.org)
  • The modeling platform developed here uncovers insights into lipid participation in antibodies' recognition of membrane proteins and highlights antibody features to prioritize in vaccine design. (elifesciences.org)
  • This valuable study reports multi-scale molecular dynamics simulations to investigate a class of highly potent antibodies that simultaneously engage with the HIV-1 Envelope trimer and the viral membrane. (elifesciences.org)
  • The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. (thermofisher.com)
  • Non-transfected (-) and transfected (+) HCT-116 whole cell extract (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1106] (GTX636321) diluted at 1:5000. (genetex.com)
  • Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1106] (GTX636321) diluted at 1:1000. (genetex.com)
  • Infection of human cells by the novel coronavirus (SARS-Cov-2) involves the attachment of the receptor binding domain (RBD) of the spike protein to the peripheral membrane ACE2 receptors. (biorxiv.org)
  • in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)
  • Type I membrane protein. (lu.se)
  • Engineered mutations to the 10E8 paratope at light-chain solvent-exposed residues that add positive charge or hydrophobic sidechains proved to increase antibody association to anionic phospholipid vesicles in vitro and also boosted neutralization potency 27 . (elifesciences.org)
  • The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. (genetex.com)
  • A molecular model of the FMDV 3A protein, derived from the nuclear magnetic resonance (NMR) structure of the poliovirus 3A protein, predicted a hydrophobic interface spanning residues 25 to 44 as the main determinant for 3A dimerization. (asm.org)
  • These replacements also led to production of infective viruses that replaced the acidic residues introduced (E) by nonpolar amino acids, indicating that preservation of the hydrophobic interface is essential for virus replication. (asm.org)
  • We identified mutations within prefu- sion F-protein antigenic sites in both subtypes. (cdc.gov)
  • 3 ). According to sequencing data from the GISAID database ( 4 ), global distribution of RSV genotypes RSV-A and -B subtypes to identify genetic mutations. (cdc.gov)
  • Omicron mutations enhance infectivity and reduce antibody neutralization of SARS-CoV-2 virus-like particles ' by Syed, A., et al. (asm.org)
  • Here, using atomistic molecular dynamics simulation, we study the correlations between the RBD dynamics with physically distant residues in the spike protein, and provide a deeper understanding of their role in the infection, including the prediction of important mutations and of distant allosteric binding sites for therapeutics. (biorxiv.org)
  • Significant research effort for drug design against COVID-19 is focused on the receptor binding domain of the spike protein, although this region is prone to mutations causing resistance against therapeutics. (biorxiv.org)
  • These residues can not only be targeted by broad spectrum antibodies and drugs, mutations in them can generate new strains of coronavirus resulting in future epidemic. (biorxiv.org)
  • 6 Significantly, the sites in S protein have been demonstrated to be vulnerable to acquire mutations. (biorxiv.org)
  • The current vaccines against COVID-19 either express the full-length S protein (AstraZeneca, CanSino Biologics, Gamaleya), or a stabilized prefusion form of the S protein with proline-stabilizing mutations and/or mutation of the furin cleavage site (Pfizer/BioNTech, Moderna, Johnson & Johnson) ( 1 - 6 ). (frontiersin.org)
  • We used next-generation sequencing to examine viral mutations and population diversity, utilizing several metrics. (cdc.gov)
  • This peptide sequence is also 100% conserved between human, mouse and rat homologs of APP, and the antibodies against this sequence cross-react with all three species. (antibodiesinc.com)
  • Therefore, when raising an anti-PTM antibody to one protein from a family, the risk is high that this antibody will also cross-react to other family members in the same sample. (eurogentec.com)
  • Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. (rndsystems.com)
  • The amino-terminal amino acid sequence of the protein (first 13 residues) matched that of the reported cDNA exactly. (aspetjournals.org)
  • The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)
  • We have developed antibodies that recognize all AMPA or all NMDA receptor variants on the surface of living neurons. (jneurosci.org)
  • To determine if the anti-PTM antibody does recognize the protein member in a specific assay, it is advised to perform a western blotting experiment, and refer to the size of the target protein member to assess its phosphorylation state. (eurogentec.com)
  • The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. (cytoskeleton.com)
  • Mutation on these residues can lead to new strains of coronavirus with different degrees of infectivity and virulence. (biorxiv.org)
  • Nonetheless, in cases of chronic inflammation and infection as in HIV, rare antibodies with lipid affinity can emerge 5 - 8 . (elifesciences.org)
  • The obtained antiserum was precipitated by saturated ammonium sulfate and then purified by Protein A affinity chromatography. (hindawi.com)
  • This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. (cytoskeleton.com)
  • The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Rac (or GTP-Cdc42) complex with glutathione affinity beads. (cytoskeleton.com)
  • Affinity purified by Protein A. (genetex.com)
  • Antibodies raised against a peptide of GPI8 also recognised recombinant GPI8, but not GPI8 in L. mexicana lysates. (gla.ac.uk)
  • antibody, raised against a peptide harboring this PTM only, may not work in a biological sample. (eurogentec.com)
  • To prove lack of written description, Sanofi sought to introduce post-priority-date evidence (evidence that did not exist until after the priority date of Amgen's patent) of additional species of antibodies (including Praluent®) and the process by which they are discovered. (klgates.com)
  • The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
  • Ultimately, the success of any potential method of inactivation must be measured by comparing infectivity in cell cultures[ 11 ] or animal models of prion disease, but western blot can be used to screen for unfolding or denaturation of PrP Sc , which would result in a loss of reactivity with prion-specific antibodies. (biomedcentral.com)
  • The main results of the team are the lack of cross-reactivity between allergens with strong sequence homologies, the relationship between the structure, resistance to proteolysis and allergenicity and the effect of technological processes and composition of the matrix on digestibility and allergenicity. (cea.fr)
  • The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)
  • The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag. (caslab.com)
  • RBPMS antibody [HL1106] detects RBPMS protein by immunohistochemical analysis. (genetex.com)
  • By Western blot, this antibody detects an ~135 kDa protein representing CRM1 from bovine retinal extract. (thermofisher.com)
  • Progress in several laboratories over the past few years including substantial partial peptide and nucleotide sequence determination has greatly enhanced our knowledge of the structural properties of this large molecule (heterodimer = 465,000 daltons). (embl.de)
  • Allergenicity of food proteins has been correlated to their structural conformation and their resistance to proteolysis. (cea.fr)
  • This topic describes the methodology developed by Just-Evotec Biologics, Inc for the structural alignment and classification of full sequences from antibodies and antibody-like structures using the Antibody Structural Numbering system (ASN). (labkey.org)
  • The viral genome encodes four structural capsid proteins (VP1 to VP4) and seven nonstructural (NS) proteins, the leader Lb/ab protease, and proteins encoded in the P2 (2B and 2C) and P3 (3A, 3B, 3C, and 3D) regions ( 9 ). (asm.org)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • One of these structural changes altered the protein so that it could no longer function for viral resistance. (usda.gov)
  • The other structural change allowed normal viral resistance to occur but affected the shape of the protein on the surface of cells. (usda.gov)
  • IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. (novusbio.com)
  • This involves replacing a GPI signal sequence with a GPI anchor in a transamidation reaction. (gla.ac.uk)
  • This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. (prosci-inc.com)
  • Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. (caslab.com)
  • One of the peptide sequences used is within the beta-amyloid fragment and recognizes the extracellular amyloid plaques observed in Alzheimer patients' brains (see Cat. (antibodiesinc.com)
  • Three other peptide sequences used, however, are within the extracellular domain of APP outside of this domain, and are useful for identifying the APP protein itself, and various proteolytic fragments, rather than the beta-amyloid peptide and plaques. (antibodiesinc.com)
  • Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. (genetex.com)
  • The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
  • Folded structures appeared in both cases with a type II' beta-turn in the parent GGIR sequence and a type I' beta-turn in the retro-inverso reGr sequence. (rcsb.org)
  • This analysis shows how a modular protein unit can be used in the evolution of large cytoskeletal structures. (embl.de)
  • Both 1D sequences and 3D structures are updated via an adaptive multi-channel equivariant encoder that is able to process protein residues of variable sizes when considering full atoms. (arxiv.org)
  • Micrococcal nucleasetreatment released these BRO proteins from the chromatin fraction,suggesting their involvement in nucleosome structures. (embl-heidelberg.de)
  • Similarities between the physical structures of avian and mammalian MHC class I glycoproteins have been proposed by comparative alignment of their amino acid sequences. (usda.gov)
  • We applied time-independent component analysis (tICA) and protein connectivity network model, on all-atom molecular dynamics trajectories, to identify key non-RBD residues, playing crucial role in the conformational transition facilitating spike-receptor binding and infection of human cell. (biorxiv.org)
  • 1 , 2 Largely expressed on the surface of SARS-CoV-2, the spike (S) protein plays a crucial role in binding to the host angiotensin-converting enzyme 2 (ACE2) through the receptor-binding domain (RBD) and facilitating viral entry, 3 , 4 which is therefore considered as one of the most preferred targets against SARS-CoV-2. (biorxiv.org)
  • Here, we describe the safety, robust immunogenicity, and potent efficacy elicited in rhesus macaques by a modified vaccinia virus Ankara (MVA) vector expressing a full-length SARS-CoV-2 spike (S) protein (MVA-S). MVA-S vaccination was well tolerated and induced S and receptor-binding domain (RBD)-binding IgG antibodies and neutralizing antibodies against SARS-CoV-2 and several variants of concern. (frontiersin.org)
  • This antibody recognizes the peptide EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the human APP gene product. (antibodiesinc.com)
  • Chickens were immunized with a keyhole limpet hemocyanin (KLH) conjugate of the synthetic peptide EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the human gene product (NP_000475.1). (antibodiesinc.com)
  • This sequence is also conserved in the mouse and rat APP gene products, as well. (antibodiesinc.com)
  • The nucleotide sequence of a fragment of the T. brucei GPI8 gene has also been obtained. (gla.ac.uk)
  • In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
  • This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. (genetex.com)
  • If, after the variable and constant regions have been detected, there remains an N-terminal portion of the query sequence that is unmatched, the N-terminal portion is aligned to germline leaders from the specified germline gene sets and also to user-specified SeqPart sequences which have been provided to the detector. (labkey.org)
  • A chicken gene that produces a protein important for viral disease resistance was obtained and its DNA sequence was determined. (usda.gov)
  • The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. (prosci-inc.com)
  • The neuraminidase (NA) gene encodes the other surface protein of the virus. (cdc.gov)
  • This rate is patterns, gene sequencing, immuno- Centre for Disease Prevention and higher than that of severe acute respira- genicity and the animal-human and hu- Control (ECDC) and the National In- tory syndrome coronavirus (SARS), man-human interfaces. (who.int)
  • To better understand these functions, we have made a set of five anti-peptide antibody reagents against APP. (antibodiesinc.com)
  • The PAK-PBD protein supplied in this kit corresponds to residues 67-150. (cytoskeleton.com)
  • Most host antibodies target 6 an- consensus sequences using SAMtools version 1.17 tigenic sites (Ø-V) of the F protein ( 10 ). (cdc.gov)
  • Each kinase will phosphorylate its substrates on amino acid patterns sharing sequence similarities, and many publications describe the consensus sequences recognized by particular kinases. (eurogentec.com)
  • If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
  • Synthetic peptide corresponding to residues C R(1043) Q A D E E K H K R Q M S V P G S(1058) of human CRM1. (thermofisher.com)
  • Akt Isoform Specific Antibody Sampler Kit provides an economical means of distinguishing between the three Akt isoforms, Akt1, Akt2 and Akt3, as well as a pan-Akt antibody. (cellsignal.com)
  • Each isoform-specific antibody recognizes endogenous levels of the specified Akt isoform independent of its phosphorylation state. (cellsignal.com)
  • Scrapie in sheep, bovine spongiform encephalopathy (BSE), chronic wasting disease (CWD) of deer and elk, and Creutzfeldt-Jakob disease in humans are chronic neurodegenerative diseases associated with the accumulation of the protease-resistant, disease-associated isoform of the prion protein (PrP Sc ) in selected regions of the central nervous system. (biomedcentral.com)
  • Human FABP6 isoform 2 contains 128 amino acid residues and is believed to be acetylated on Ala2. (rndsystems.com)
  • Bulk Order Inquiry for Anti-Amyloid Precursor Protein, Peptide #5 Antibody ------- (please add any order requirements, including desired quantity, timing, etc. (antibodiesinc.com)
  • Amyloid Precursor Protein (APP, UniProt Accession Number P05067) is a 770 amino acid, single-pass transmembrane protein whose beta-amyloid proteolytic fragment can form neurotoxic extracellular accumulations in human cerebral cortex, and is widely believed to be the cause of Alzheimer's dementia. (antibodiesinc.com)
  • The precursor protein (pre-IL-33) was cleaved by caspase-1 into a mature 18-kDa protein (IL-33) in in vitro experiments using a recombinant protein [ 2 ]. (hindawi.com)
  • Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. (cellsignal.com)
  • Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins, and extracts from various cell lines, using Akt (pan) (C67E7) Rabbit mAb. (cellsignal.com)
  • Western blot analysis of extracts from Akt1 (lane 1), Akt2 (lane 2) and Akt3 (lane 3) knock-out mouse embryonic fibroblasts (MEFs) and matched wild-type MEFs (lane 4) using Akt1 (C73H10) Rabbit mAb (upper) and Akt2 Antibody #2962 (lower). (cellsignal.com)
  • Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins using Akt1 (C73H10) Rabbit mAb (upper) and Akt (pan) (11E7) Rabbit mAb #4685 (lower). (cellsignal.com)
  • The protein was recognized by rabbit antibodies raised against rat P-450 2A1, and a rabbit antiserum against the P-450 2A6 preparation was also prepared. (aspetjournals.org)
  • Hence, as with the erythrocyte lipid raft protein stomatin, podocin is present in high-order oligomers and may serve a scaffolding function. (jci.org)
  • Ancillary protein-lipid contacts reveal surprising contributions from antibody framework regions. (elifesciences.org)
  • However, the evidence for rules for lipid recognition in antibodies is still incomplete . (elifesciences.org)
  • Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. (rndsystems.com)
  • Antibody design is an essential yet challenging task in various domains like therapeutics and biology. (arxiv.org)
  • In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). (thermofisher.com)
  • The perceived importance of GPI-anchored proteins makes the GPI biosynthetic pathway a good target for anti-parasite chemotherapy. (gla.ac.uk)
  • for example, kinases such as MAP kinases, JAK, PI3K and many others, when activated upon a specific stimulus in a specific cellular context, will not phosphorylate one target only, but usually a subset of different protein targets which will collectively lead to the expected stimulation. (eurogentec.com)
  • Amgen's patents share a common specification and are directed toward antibodies that help rid the body of "bad cholesterol" or "low-density lipoprotein" cholesterol known as LDL-C. Those antibodies target PCSK9, a naturally occurring protein, and prevent PCSK9 from binding to and destroying liver cell receptors (LDL-R) that help extract LDL-C from the bloodstream. (klgates.com)
  • Large scale screening of therapeutic molecules and antibodies are underway aiming to target the spike protein and consequently prevent infection. (biorxiv.org)
  • These antibodies detected recombinant protein but failed to detect protein in L. mexicana cell lysates. (gla.ac.uk)
  • A protein of 38-40 kDa, however, was detected in cell lysates of T. brucei with the antirecombinant GPI8 antibodies. (gla.ac.uk)
  • Deciphering protein–protein interactions. (crossref.org)
  • Genes predicting interactions with host cellular mechanismsinclude homologues of the inhibitor of apoptosis protein, stress responseprotein phosphatase 2C, extracellular matrixin metalloproteases,ubiquitin, calcium binding EF-hand protein, glycosyltransferase, and atriacylglyceride lipase. (embl-heidelberg.de)
  • Replacements that favored (Q44R) or impaired (Q44D) the polar interactions predicted between residues Q44 and D32 did not abolish dimer formation of transiently expressed 3A, indicating that these interactions are not critical for 3A dimerization. (asm.org)
  • These proteins display interactions and are involved in the regulation of the cellular cytoskeleton. (biomedcentral.com)
  • These studies aid in understanding how genes determining cell surface proteins can affect interactions between cells and confer viral disease resistance. (usda.gov)
  • IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑23 Antibody. (rndsystems.com)
  • a ) Amino acid sequence comparison of human and mouse podocin. (jci.org)
  • Most of the amino acid exchanges between human and mouse are restricted to the NH 2 -terminal part of the protein, whereas the tm domain, the stomatin signature, and the COOH-terminal part are almost identical. (jci.org)
  • Purified P-450 2A6 catalyzed coumarin 7-hydroxylation and 7-ethoxycoumarin O-deethylation at rates similar to those measured in the human liver sample used to prepare P-450 2A6, and these two microsomal activities were strongly inhibited by the antibodies. (aspetjournals.org)
  • Human P-450 2A6 did not catalyze testosterone 7 alpha-hydroxylation, a characteristic activity of the related rat P-450 2A1 protein. (aspetjournals.org)
  • The predicted protein shares 31% identity with yeast and human homologues. (gla.ac.uk)
  • Sequence comparison of L. mexicana, yeast and human GPI8 proteins identified two potential active site cysteine residues. (gla.ac.uk)
  • The amino acid sequence of human FABP6 is 80%, 78%, and 75% aa identical to that of mouse, canine, and porcine FABP6, respectively. (rndsystems.com)
  • Phylogenetic analysis characterized the sequences into two discrete clusters representing two of the four global lineages of human G1 VP7 proteins. (microbiologyresearch.org)
  • Sequence homology between human and animal rotavirus serotype-specific glycoproteins. (microbiologyresearch.org)
  • Recombinant protein encompassing a sequence within the center region of human RBPMS. (genetex.com)
  • 18 The human immune system started generating antibodies specific to residues outside RBD even at the earlier stage of the pandemic. (biorxiv.org)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human FGF12 (NP_004104.3). (prosci-inc.com)
  • Akt Antibody recognizes endogenous levels of all three Akt isoforms. (cellsignal.com)
  • This tag facilitates the detection, isolation, and purification of the proteins. (caslab.com)
  • SAMHD1 is acetylated at residue lysine 405 (K405) in vitro and in vivo by an acetylatransferase, arrest defective protein 1 (ARD1). (oncotarget.com)
  • Acetylated SAMHD1 wildtype proteins have enhanced dNTPase activity in vitro , whereas non-acetylated arginine substituted mutants (K405R) do not. (oncotarget.com)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • They contain distinct amino- and carboxy-terminal domains (Bro-N and Bro-C, respectively) that are present independently of each other and in distinct contexts in a variety of other viral proteins. (embl-heidelberg.de)
  • SAM domain and HD domain containing protein 1 (SAMHD1) is a deoxynucleotide triphosphohydrolase (dNTPase) that inhibits retroviruses by depleting intracellular deoxynucleotide triphosphates (dNTPs) in non-cycling myeloid cells. (oncotarget.com)
  • NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)
  • The deduced amino acid sequences of the outer capsid protein, VP7, of serotype G1 rotavirus clinical isolates collected over a 6 year period (1990-1995) in Melbourne, Australia, were examined. (microbiologyresearch.org)
  • The major conformational difference is localized to the GR residues of the first type I b turn. (usda.gov)