• Naïve ( A and B ) and activated ( C and D ) T cells were assayed for their chemotactic response to serum samples obtained from patients undergoing therapy with trastuzumab and IL-12. (aacrjournals.org)
  • E, migration of activated T cells was measured in response to patient serum that had been pretreated with neutralizing concentrations of antibodies to IL-8, RANTES, and MIP-1α or with isotype-matched control IgG. (aacrjournals.org)
  • Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool. (pubmedcentralcanada.ca)
  • Additionally, many (commonly available) cell lines used for transient transfection are serum-dependent, resulting in contamination of produced mAbs by bovine IgGs. (pubmedcentralcanada.ca)
  • Here, we decided to take advantage of a biotechnology-relevant production cell line, Chinese hamster ovary (CHO) DG44, to establish a serum-free, stable, CR9114-like (CR9114L, a generic version of CR9114) antibody-producing cell line for a steady supply of this mAb with low batch to batch variation. (pubmedcentralcanada.ca)
  • The Kelsoe group will analyze induction of germinal centers, Ig class switch recombination and hypermutation, affinity maturation of serum antibodies and germinal center B cells in response to MPER immunogens. (grantome.com)
  • Such antibodies may sometimes not be detected in recipient serum. (elsevier.com)
  • Peripheral blood B cells were obtained from 36 subjects, including 16 allograft recipients, 12 sensitized patients, three multiparous women with serum HLA antibodies, and five healthy non-transfused male subjects. (elsevier.com)
  • Beneficial effect of 30Kc6 gene expression on production of recombinant interferon-β in serum-free suspension culture of CHO cells", Process Biochemistry, Feb. 2009, vol. 44, pp. 146-153. (freepatentsonline.com)
  • In two cases, serum antibodies to Arg-hgp and Lys-hgp were AlphaScreen positive, whereas plasma cells were scarcely observed within the lesions. (fujita-hu.ac.jp)
  • Introduction: Naturally occurring antibodies (auto-Abs) recognizing human granulocyte-colony stimulating factor were detected with high frequency in serum samples obtained from umbilical cord blood of newborns (12 of 65 samples screened) and maternal peripheral blood serum samples from women at the end of gestation (seven of 56 cases tested). (unipi.it)
  • We now report that the antibodies produced by the B-cells within these clusters, as well as those in the serum of patients who were rejecting transplants, react strongly with the core carbohydrate epitopes of bacterial lipopolysaccharide (LPS). (pnas.org)
  • The results, published Tuesday, July 14 in Immunity , could provide vaccine designers with a goalpost when aiming for long-lasting antibody production. (emory.edu)
  • As described in part of the Immunity paper, the researchers studied 11 older individuals (aged 43 to 70) who had not been immunized against measles or mumps, but who had antibodies in their blood indicating infection by those viruses in childhood. (emory.edu)
  • Regarding its function in the immune system, IL-6 is now thought of as an important link between innate and adaptive immunity, mediating several aspects of B and T cell responses ( 3 ). (rupress.org)
  • Memory is the hallmark of the acquired immunity, which allows for rapid expansion of T- and B-cell responses upon re-exposure to an immunogen. (mainebiotechnology.com)
  • Antibodies are an essential component of our immune system, underpinning the effectiveness of both the primary immune response to microbial pathogens and the protective and long-lived immunity against. (bioportfolio.com)
  • Plasma cell survival and the consequent duration of immunity vary widely with infection or vaccination. (bioportfolio.com)
  • The progress of the disease may be broken down into three phases: an initial phase, characterized by a Type IV immune response (Cell-Mediated Immunity, delayed-hypersensitivity response), a second phase, characterized by a Type III response (Immune Complex), and a third phase where substantial tissue damage occurs. (angelfire.com)
  • Lachmann outlines a scheme which attempts to account for most of the immunological findings in ROU and BS, and the shift from an early, predominantly lymphomonocytic infiltration (probably Type IV, cell- mediated immunity (CMI), delayed hypersensitivity) to a later PMNL infiltration with features associated with a Type III (immune complex) reaction. (angelfire.com)
  • Here the authors show that PRL-3 is also detectable on cell surface, and can be recognized by PRL3-zumab to recruit immune cells into tumor to promote anti-tumor immunity, thereby implicating PRL-3 as a potential tumor antigen. (nature.com)
  • a key feature of this response is the production of antibodies by B cells (or B lymphocytes) involving an arm of the immune system known as humoral immunity . (wikipedia.org)
  • The benefit of this method of producing antibody is that it doesn't require initial infection, and through this route, many vaccinations confer immunity without causing illness. (livestrong.com)
  • Their prophylactic and therapeutic protection ability was first discovered in the late nineteenth century by the passive transmission of antibodies from a diseased animal that provided immunity against diphtheria. (frontiersin.org)
  • an antibody-secreting cell, derived from B cells, that plays a major role in antibody-mediated immunity. (dictionary.com)
  • The antibodies act to protect the host from future exposure to the antigen (immunity). (faqs.org)
  • Interestingly, humans have more memory B cells than mice 3,4 , although this may be an artifact of mice being housed in pathogen-free facilities and therefore, are exposed to only a limited number of microbes and infectious agents. (mainebiotechnology.com)
  • Memory cells need to be recruited to lymph nodes if the infectious agent induces local inflammation. (mainebiotechnology.com)
  • These cells defend the body against infection and disease by ingesting foreign materials and debris, by destroying infectious agents and cancer cells, or by producing antibodies (Britannica 1). (artspace-jhb.co.za)
  • Yet with billions of dollars of research funds, all the infectious disease industry could do this flu season is produce a vaccine that was only 36% effective . (lewrockwell.com)
  • In this study, we investigated the response of breast cancer cells and xenografts to combination treatment with TRA-8 and chemotherapy and the mechanisms associated with the induction of apoptosis. (aacrjournals.org)
  • If you've tried at least two traditional treatments for primary mediastinal B-cell lymphoma (different combinations of chemotherapy drugs, plus the monoclonal antibody rituximab [Rituxan] and radiation) and they haven't worked, or your cancer has come back, CAR T-cell therapy is a new option your doctor might recommend. (webmd.com)
  • Chemotherapy is the use of drugs to kill cancer cells. (upstate.edu)
  • Interferon may be used with chemotherapy to help prolong remission, slowing the speed at which myeloma cells grow. (upstate.edu)
  • Both radiotherapy and systemic treatments with chemotherapy and anti-CD20 antibodies are efficacious and thus the experience of individual centers and each patient's preferences in terms of adverse effects are important parameters in the decision process. (bloodjournal.org)
  • Development of monoclonal antibodies (mAb) as therapeutics in the biopharmaceutical industry, including biosimilar or biobetter versions of marketed mAbs has increased tremendously over the last ten years [ 1 , 2 , 3 ]. (mdpi.com)
  • Monoclonal antibodies (mAbs) are highly specific affinity reagents used for detecting and treating diseases. (justia.com)
  • The standard method for creating mAbs to a particular antigen involves the creation of a fused cell called a "hybridoma. (justia.com)
  • In addition, many of the clones generated may not produce mAbs that recognize the antigen of interest. (justia.com)
  • Therapeutic monoclonal antibodies (mAbs) have evolved into an important class of effective medicine in treatment of various diseases. (frontiersin.org)
  • Although the plant-based expression system has been tested to produce fully assembled recombinant mAbs, coordinately expressing HC and LC at similar levels in a transgenic plant remains a challenge. (frontiersin.org)
  • Once HC Ig gene rearrangement has successfully initiated, the cells become precursor B (or pre-B) cells. (mainebiotechnology.com)
  • During the pre-B cell stage (which can be divided into different phases) HC Ig gene rearrangement is completed and tested (I'll get back to this in a moment) and light chain (LC) gene rearrangement takes place to generate a fully functional B cell receptor (BCR, which is a membrane bound antibody). (mainebiotechnology.com)
  • Our assertion that ANCA is a marker for a mucosal disease-related immune response predicts the existence of ANCA producing B cell clones in the lamina propria lymphocyte (LPL) fraction of UC patients. (jimmunol.org)
  • These findings indicate the existence of pANCA-producing B cell clones in mucosal lesions of UC patients and support our hypothesis that pANCA production is a consequence of a mucosal immune response specific to UC. (jimmunol.org)
  • The immune response mounted in response to the antigen chelate produces antibodies that. (google.com)
  • The immune response mounted in response to the antigen chelate produces antibodies that are capable of binding both a substrate and a metal ion, thus achieving a metal cofactor assisted reaction. (google.com)
  • Certain cells from a mother persist in their children's bodies and can provoke an immune response in which the child's body attacks itself, according to Mayo Clinic research published in the current issue of the Journal of Immunology (http://www.jimmunol.org). (news-medical.net)
  • Consequently, an effective immune response often involves the production of many different antibodies by many different B cells against the same antigen. (wikipedia.org)
  • When the body discovers such a substance several kinds of cells go into action in what is called an immune response. (chemocare.com)
  • There are different types of white blood cells that are part of the immune response. (chemocare.com)
  • One very clever way to elicit production of antibody is to expose an individual to parts of a virus or bacterium without exposing them to the entire living pathogen. (livestrong.com)
  • A preferred receptor is human CD152, particularly the regions of CDR1, CDR2 and CDR3 that elicit antibodies serving as antagonist, inverse agonist and agonist, respectively. (freepatentsonline.com)
  • Glycosylation heterogeneities have been well documented to affect biological activities such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) through their interaction with Fc-receptors. (mdpi.com)
  • Adriamycin or Velcade, a proteosome inhibitor, in combination with TRA-8 produced additive cytotoxicity against TRA-8 sensitive 2LMP breast cancer cells and synergism against LCC6 cells and the BT474 resistant line. (aacrjournals.org)
  • In summary, TRA-8 in combination with Adriamycin or Velcade produced additive or synergistic cytotoxicity and activation of caspases 3, 8 and 9 in breast cancer cells. (aacrjournals.org)
  • FUT8(-/-) CHO-K1 cells which increases antibody-dependent cellular cytotoxicity (ADCC) has been widely used as hosts for antibody production. (creative-biogene.com)
  • We have also examined intraclonal affinity maturation by generating Abs inferred to have been produced by the unmutated precursors and branchpoint intermediates of the largest 68ld clone. (jimmunol.org)
  • Affinity maturation takes place in the germinal center and the result of this process is the generation of B cell populations that produce antibodies and memory. (mainebiotechnology.com)
  • In this article, we will explore what happens to activate memory and antibody production following the affinity maturation in the germinal center. (mainebiotechnology.com)
  • in 2012 in a study describing broadly neutralizing human antibodies against influenza B virus strains [ 1 ]. (pubmedcentralcanada.ca)
  • MAb CR9114 is using the V H 1-69 germline gene and compared to other broadly neutralizing antibodies has a relatively small number of somatic mutations [ 1 ]. (pubmedcentralcanada.ca)
  • With that said, three of the most broadly neutralizing antibodies (BNABs) isolated from human B cells (4E10, 2F5, Z13e1) are directed at the uniquely accessible yet highly conserved segment of gp41 known as the membrane proximal ectodomain region (MPER). (grantome.com)
  • A multivalent vaccine that presents diverse influenza virus subtype H1 hemagglutinins on its surface induces broadly neutralizing antibodies in an animal model by displaying conserved epitopes at higher density than strain-specific epitopes. (nature.com)
  • Supernatants from NK cells costimulated with immobilized antibody plus cytokine induce chemotaxis of naïve and activated T cells. (aacrjournals.org)
  • We show that IL-6 is sufficient and necessary to induce IL-21 production by naive and memory CD4 + T cells upon T cell receptor stimulation. (rupress.org)
  • will rapidly proliferate, differentiate into plasma cells, and induce the generation of a germinal center 3 . (mainebiotechnology.com)
  • RAS serves as a major communication hub that relays information from outside the cell to as many as 12 different signaling pathways inside the cell, including the MAPK and PI3K pathways, which then collectively induce changes to our cells. (eurekalert.org)
  • By combining Chk1 inhibitors with another targeted agent, such as Src inhibitors, we were able to induce cell death in multiple myeloma cells while sparing healthy, normal cells. (bio-medicine.org)
  • The cause is unknown but the current thinking is that antibodies are produced against endothelial cells in tiny arteries which leads to damage and the symptoms related to the illness. (wikipedia.org)
  • The latest thinking is that an antibody directed against endothelial cells is the pathogenic mechanism in this disease which causes the microscopic strokes in the brain, retina, and inner ear. (wikipedia.org)
  • In various embodiments, the present invention is drawn to antibodies or antigen-binding fragments thereof that bind to a vertebrate high mobility group box (HMGB) polypeptide, methods of detecting and/or identifying an agent that binds to an HMGB polypeptide, methods of treating a condition in a subject. (google.ca)
  • The antigen-binding site of the antibody has a unique structure that allows it to bind antigen in a highly specific manner. (nature.com)
  • Antibodies bind antigen with the help of a VHH fragment that can identify specific and unique conformational epitopes by the presence of its long complementary determining regions (CDR3). (frontiersin.org)
  • Each antibody is designed to recognize and bind only with a specific antigen, and doctors have identified a host of cancer-specific antibodies that can be used to kill cancer cells. (nanotech-now.com)
  • Using "helper" T-cells instead of B-cells that target specific intruders, the new vaccine provokes the immune system to go after the virus with its own defenses. (healthline.com)
  • There are five different antibody isotypes that are responsible for different parts of the immune system reaction to foreign matter. (wanttoknowit.com)
  • Such substances are known as soluble antibodies and perform important functions in countering infections. (wikipedia.org)