• After obstructing in 5%BSA, the membranes were incubated with appropriate dilutions of specific primary antibodies over night at 4?C, followed by incubation of secondary antibody for 1?h at space temperature. (cancerdir.com)
  • Anti-rabbit and anti-mouse secondary antibodies 10058-F4 conjugated to TRITC or Cy5 were purchased from Jackson ImmunoResearch Laboratories. (conferencedequebec.org)
  • Polyclonal antibodies offers greater sensitivity for detecting proteins that are present in low expression levels, as the target protein will bind more than one antibody molecule on its multiple epitopes (this is less advantageous for quantification experiments). (cusabio.com)
  • Endogenous TNFAIP2 protein was immunoprecipitated using an anti-TNFAIP2 antibody. (bioz.com)
  • Recently, we have found that LGR5 interacts with the scaffold protein IQGAP1 (IQ Motif Containing GTPase Activating Protein 1) to increase cell-cell adhesion in colon cancer cells and knockdown of LGR5 significantly enhanced drug resistance and activation of proteins associated with survival signaling. (grantome.com)
  • Factors secreted by cells expressing dominant-active Gα12 were identified by protein array, and their involvement in breast cancer cell invasion was assessed through both RNAi-mediated knockdown and antibody neutralization approaches. (biomedcentral.com)
  • Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (microarraystation.com)
  • Human IgG antibody Laboratories manufactures the s100b elisa reagents distributed by Genprice. (microarraystation.com)
  • Although an ELISA-based method was used, the readout reflects the activity rather than the binding of the transcription factors because the primary antibody provided with this kit is specific for an epitope on the bound and active form of the transcription factor. (syksignaling.com)
  • The plant-orange matter is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. (worldcarecouncil.org)
  • Each of these individual antibodies recognizes a unique epitope that is located on that antigen. (cusabio.com)
  • Correlative live cell-immunofluorescence microscopy was performed to determine localization of WAVE2 and IQGAP1 to protruding versus retracting sides. (conferencedequebec.org)
  • Activation of PKC corrected problems in WAVE2 and IQGAP1 localization cell distributing and cell shape to levels observed in control cells but did not correct problems in cell migration. (conferencedequebec.org)
  • Humanized anti-CD123 antibody facilitates NK cell antibody-dependent cell-mediated cytotoxicity (ADCC) of Hodgkin lymphoma targets via ARF6/PLD-1. (rndsystems.com)
  • Our work will further provide proof-of-concept for the development of innovative antibody-drug conjugates to target and destroy colon tumors and cancer stem cells. (grantome.com)
  • The role of LGR5-IQGAP1 interaction in regulating the different properties that define CSCs will also be examined. (grantome.com)
  • These effects of Cdk5 inhibition or suppression were accompanied by decreased Rac activity, increased Rho activity, and enhanced binding of E-cadherin to the Rac effector Ras GTPase-activating-like protein (IQGAP1). (molvis.org)
  • Tyrosine phosphorylation of the scaffold protein IQGAP1 in the MET pathway alters function. (nih.gov)
  • Ubiquitination of the scaffold protein IQGAP1 diminishes its interaction with and activation of the Rho GTPase CDC42. (nih.gov)
  • Zhang M, Li Z, Jang H, Hedman AC, Sacks DB , Nussinov R. Ca2+-dependent switch of calmodulin interaction mode with tandem IQ motifs in the scaffolding protein IQGAP1. (nih.gov)
  • Exactly how CaM binds the IQ motifs and which functions of IQGAP1 CaM regulates and how are fundamental mechanistic questions. (nih.gov)
  • IQGAP1, a scaffolding protein of the adherens junctions interacting with E-cadherin, regulates cellular plasticity and proliferation. (oncotarget.com)
  • 17. IQGAP1-dependent signaling pathway regulates endothelial cell proliferation and angiogenesis. (nih.gov)
  • Endothelial IQGAP1 regulates leukocyte transmigration by directing the LRBC to the site of diapedesis. (nih.gov)
  • IQ domain GTPase-activating scaffolding protein 1 (IQGAP1) mediates cytoskeleton, cell migration, proliferation, and apoptosis events. (nih.gov)
  • IQGAP1 knockout mice develop gastric cancer, but the IQGAP1 protein is associated with some advanced-stage human cancers. (nih.gov)
  • Expression of LGR5 and phosphorylation ratio of IQGAP1 showed opposite trends in breast cancer and normal body. (researcherslinks.com)
  • Viability, migration and invasion ability of breast cancer cells were all affected by LGR5 expression and IQGAP1 phosphorylation ratio. (researcherslinks.com)
  • LGR5 affected IQGAP1 phosphorylation process by linking to IQGAP1 through its own C-terminus. (researcherslinks.com)
  • This study proved that LGR5, which is highly expressed in breast cancer cells, interacts with IQGAP1 through its C-terminal end, reducing the phosphorylation ratio of IQGAP1, thereby improving the viability, migration and invasion ability of breast cancer cells, and ultimately increasing the malignant transformation rate of breast cancer. (researcherslinks.com)
  • IQGAP1 causes choroidal neovascularization by sustaining VEGFR2-mediated Rac1 activation. (nih.gov)
  • We also measured the expression of IQGAP1 at both mRNA and protein levels in different IQGAP1 genotypes. (nih.gov)
  • IQGAP1 binds AMPK and is required for maximum AMPK activation. (nih.gov)
  • IQGAP1 binds the Axl receptor kinase and inhibits its signaling. (nih.gov)
  • Ca 2+ promotes IQ-CaM interactions and increases the amount of IQGAP1-loaded CaM for IQGAP1-mediated signaling. (nih.gov)
  • The aim of this study was to elucidate the consequences of IQGAP1 deletion on H. pylori -induced gastric carcinogenesis. (oncotarget.com)
  • IQGAP1 and E-cadherin expression was evaluated in gastric tissues and in gastric epithelial cell lines in response to H. pylori infection. (oncotarget.com)
  • H. pylori infection induced IQGAP1 and E-cadherin delocalization from cell-cell junctions. (oncotarget.com)
  • In vitro , knock-down of IQGAP1 favoured the acquisition of a mesenchymal phenotype and CSC-like properties induced by H. pylori infection. (oncotarget.com)
  • Our results indicate that alterations in IQGAP1 signalling promote the emergence of CSCs and gastric adenocarcinoma development in the context of an H. pylori infection. (oncotarget.com)
  • The majority of mechanistic studies have been conducted in IQGAP1 the cellular level and show DNA-built carriers primarily interact with scavenger receptors [5, 6]. (bso14.org)
  • In mice, IQGAP1 deficiency leads to gastric hyperplasia. (oncotarget.com)
  • Transgenic mice deleted for iqgap1 and WT littermates were infected with Helicobacter sp. (oncotarget.com)
  • After one year of infection, iqgap1 +/- mice developed more preneoplastic lesions and up to 8 times more gastro-intestinal neoplasia (GIN) than WT littermates. (oncotarget.com)
  • Six days after tumor injection, when the xenograft grew to approximately 100 AZD-3965 mm3, the mice were divided randomly into IQGAP1 four groups: a negative control group, which was to be treated with PBS (n? (nonamimaho.com)
  • We asked whether IQGAP1 expression is associated with breast cancer development and whether genetic variants at the miR-124 binding site are important. (nih.gov)
  • We genotyped the IQGAP1 SNP rs1042538 A/T in 1,541 breast cancer cases and 1,598 controls and analyzed the frequency of the variant and interactions with major risk factors in these populations. (nih.gov)
  • This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule. (thermofisher.com)
  • The expression levels of IQGAP1 protein were significantly higher in the TT genotype compated to the AA genotype. (nih.gov)
  • The relative expression levels of LGR5 and phosphorylated IQGAP1 were detected by Western blot. (researcherslinks.com)
  • Absence of IQGAP1 protein leads to insulin resistance. (nih.gov)
  • Su G, Atakilit A, Li JT, Wu N, Luong J, Chen R, Bhattacharya M , Sheppard D. (2013) Effective treatment of mouse sepsis with an inhibitory antibody targeting integrin αvβ5. (ucsf.edu)
  • In addition, specific focusing on of DNA-built drug service providers using affinity moieties such as antibodies (Abdominal muscles), aptamers, vitamins, etc. offers been shown to enable preferential connection with cells expressing the selected receptors or disease markers. (bso14.org)
  • Anti-CD45-APC antibody was utilized for the double staining determination of the C24D+/CD45+?cells. (nonamimaho.com)
  • For the determination of the activated PBMCs sub-populations, PBMCs from your co-cultures with tumor cells were counted and transferred to a FACS tube (0.5×106 cells/50?l PBS) and incubated with the following antibodies: CD4-PC7, CD8-KO, CD56-PE, NKG2D-AF750, CD14-KO, CD69-PC5, CD57-FITC, CD137-ECD (Beckman Coulter). (nonamimaho.com)
  • The CD45+?cells were identified by FACS analysis with an anti-CD45-PB antibody (Supplementary Physique 2). (nonamimaho.com)