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  • Hybridoma
  • Generation of a murine monoclonal antibody specific for N-glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids," Hybridoma , vol. 14, no. 6, pp. 551-556, 1995. (hindawi.com)
  • Immunogenetic analysis of variable regions encoding AB1 and gamma-type AB2 antibodies from the NeuGc-containing ganglioside family," Hybridoma , vol. 20, no. 4, pp. 211-221, 2001. (hindawi.com)
  • Initially, murine antibodies were obtained by hybridoma technology, for which Jerne, Köhler and Milstein received a Nobel prize. (wikipedia.org)
  • Reactivity
  • Such data is valuable for investigation of cross-reactivity between known allergens and analysis of potential allergenicity in proteins. (wikipedia.org)
  • mice
  • F. Stäb, F. Austrup, and E. Kölsch, "Regulation of the anti-alpha(1----3) dextran IgG antibody response of BALB/c mice by idiotype-specific T suppressor lymphocytes," Journal of Immunology , vol. 144, no. 1, pp. 53-59, 1990. (hindawi.com)
  • Synthesis, Characterization, and Immunological Properties in Mice of Conjugates Composed of Detoxified Lipopolysaccharide of Salmonella paratyphi A Bound to Tetanus Toxoid, with Emphasis on the Role of O Acetyls",Infect.Immun. (patentgenius.com)
  • A structurally-related benzoyl derivative was synthesized to facilitate coupling to NNK-carrier proteins, which were characterized for the presence of the N -nitroso group using the Griess reaction, and used to immunize BALB/c mice. (mdpi.com)
  • Splenocytes from mice bearing NNK-specific antibodies were used to create hybridomas. (mdpi.com)
  • Polyclonal or monoclonal antibody protocols- choose one of six species options (eight immunization schedules) for polyclonal antibody production, or one of three package sizes (number and strains of mice) for monoclonal antibody production. (thermofisher.com)
  • Synthesis
  • Add to this the complexity of the numerous proteins involved, not only as carriers of carbohydrate, the glycoproteins, but proteins specifically involved in binding and reacting with carbohydrate: Carbohydrate-specific enzymes for synthesis, modulation, and degradation Lectins, carbohydrate-binding proteins of all sorts Receptors, circulating or membrane-bound carbohydrate-binding receptors To answer this question one should know the different and important functions of glycans. (wikipedia.org)
  • Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism. (wikipedia.org)
  • toxoid
  • Immunogens Consisting of Oligosaccharides from the Capsule of Haemophilus influenzae Type b Coupled to Diphtheria Toxoid or the Toxin Protein CRM197",J. Clin. (patentgenius.com)
  • Interactions
  • The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. (abcam.com)
  • The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab15556, 1µg/1x10 6 cells) for 30 min at 22ºC. (abcam.com)
  • The first step in this proposed model is an interaction with the unfolded fusion protein (TAT) and the membrane through electrostatic interactions, which disrupt the membrane enough to allow the fusion protein to cross the membrane. (wikipedia.org)
  • It aims to study the complex protein-protein interactions and networks and allows a better understanding of immune responses and their role during normal, diseased and reconstitution states. (wikipedia.org)
  • There are also some computational models which focus on protein-protein interactions and networks. (wikipedia.org)
  • Biotin binds tightly to the tetrameric protein avidin (also streptavidin and neutravidin), with a dissociation constant Kd on the order of 10−15 M, which is one of the strongest known protein-ligand interactions. (wikipedia.org)
  • sequence
  • Antibodies are created against the protein-drug complex, leading to the destructive sequence described above. (wikipedia.org)
  • The autoantibody model proposes that, through a mechanism not yet understood, certain drugs will cause antibodies to be made against red blood cells which again leads to the same destructive sequence. (wikipedia.org)
  • Computational immunology is a branch of bioinformatics and it is based on similar concepts and tools, such as sequence alignment and protein structure prediction tools. (wikipedia.org)
  • In eukaryotes, transcription is performed by three types of RNA polymerases, each of which needs a special DNA sequence called the promoter and a set of DNA-binding proteins-transcription factors-to initiate the process. (wikipedia.org)
  • They occur if polyadenylation signal sequence (5'- AAUAAA-3') is present in pre-mRNA, which is usually between protein-coding sequence and terminator. (wikipedia.org)
  • Invitrogen
  • If an Invitrogen™ antibody doesn't perform as described on our website or datasheet, we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
  • eukaryotic
  • While transcription of prokaryotic protein-coding genes creates messenger RNA (mRNA) that is ready for translation into protein, transcription of eukaryotic genes leaves a primary transcript of RNA (pre-mRNA), which first has to undergo a series of modifications to become a mature mRNA. (wikipedia.org)
  • cellular
  • Protein phosphorylation provides a signalling system that can be thought of as a kind of protein on/off switch for many cellular signalling pathways. (abcam.com)
  • Cellular networks underlying phosphorylation can be very complex and often occurs on multiple distinct sites on a given protein. (abcam.com)
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. (wikipedia.org)
  • The four known IgG subclasses are involved in antibody-dependent cellular cytotoxicity. (wikipedia.org)