Antibody cadherins. Medical search. Frequent questions

PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). (rndsystems.com)
Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. (rndsystems.com)

The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. (origene.com)
CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. (biolegend.com)
Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. (rndsystems.com)
Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. (rndsystems.com)
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. (ecmbio.com)
Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). (bio-techne.com)
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. (enquirebio.com)
The transmembrane adhesion proteins in these cell-matrixjunctions are integrins a large family of proteins distinct from the cadherins.Focal adhesions enable cells to get a hold on the extracellular matrix through integrins that link intracellularly to actin filaments. (ostatic.com)

This gene is a classical cadherin from the cadherin superfamily. (origene.com)
P-Cadherin is a classical cadherin molecule. (rndsystems.com)
This gene encodes a classical cadherin of the cadherin superfamily. (ebiocell.com)

Immunogen Recombinant human E-cadherin. (qedbio.com)
A portion of amino acids 60-110 of human ID2 was used as the immunogen for this antibody. (novusbio.com)

ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)

Biological Function Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). (qedbio.com)
Abcam: antibodies, proteins, kits. (abcam.com)
Affected individuals make antibodiesagainst one of their own desmosomal cadherin proteins. (ostatic.com)
We investigated the use of e-cadherin immunofluorescence in different species (rat and mouse), with different fixatives (10% neutral buffered formalin, 4% paraformaldehyde, and 2.5% glutaraldehyde ), with and without antigen retrieval, and as a dual label with immunofluorescence for other proteins. (cdc.gov)
E-cadherin immunofluorescent double labeling can also be used with activated caspase 3 or â-catenin immunofluorescence to localize the expression of these proteins in damaged airways. (cdc.gov)
135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules, designated CDH3. (rndsystems.com)
E-cadherin (E-cad, Cdh1) is the founding member of the cadherin gene family and is essential for the integrity of epithelial tissues. (biomedcentral.com)

Homo sapiens cadherin 1 (CDH1), transcript variant 1, mRNA. (origene.com)
Background E-cadherin is a calcium-dependent intercellular adhesion molecule encoded by the CDH1 gene and present on epithelial cells. (qedbio.com)
In practical applications, E-cadherin is used as a phenotypic marker of the lobular subtype of cancer, where loss of hetero zygosity in chromosome region 16q22.1, the gene region encoding E-cadherin ( CDH1 ) is frequent ( 6 ). (iiarjournals.org)

Detects mouse P-Cadherin in direct ELISAs and Western blots. (rndsystems.com)
E-Cadherin Mouse Monoclonal Antibody detects endogenous levels of E-Cadherin protein. (ebiocell.com)
In western blots, the antibody detects a 120 kDa band corresponding to E-cadherin in human A431 cells, and does not detect VE-cadherin or N-Cadherin. (ecmbio.com)
Anti-Vimentin Antibody detects endogenous levels of total Vimentin protein. (sigmaaldrich.com)

This association links the cadherin protein to the cytoskeleton. (rndsystems.com)
Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. (rndsystems.com)
Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. (enquirebio.com)
In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)
Sections were immunostained for estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her2), basal cytokeratin CK5, nuclear protein Ki67 and E-cadherin with a Leica Bond-Max autostainer. (iiarjournals.org)
Additionally, overexpression of ARG1 led to a significant increase in the expression of Vimentin, N-cadherin, and β -catenin both at protein and mRNA levels, which promotes the EMT process. (hindawi.com)
Besides, the level of E-cadherin protein was upregulated in ARG1 knocked down cells. (hindawi.com)
The association between loss or down-regulation of E-cadherin, an epithelial cell-cell adhesion protein, and progression of breast cancer has been extensively documented [ 3 , 4 ]. (biomedcentral.com)
Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948 ) coated on a Clear Polystyrene Microplate (Catalog # DY990 ). (rndsystems.com)
Objective: To establish the correlation of the clinical staging of epidermoid intrabuccal carcinoma with the expression pattern of the E-cadherin protein. (bvsalud.org)

In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. (roche.com)
Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. (roche.com)

Specificity Human E-cadherin. (qedbio.com)

Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. (elifesciences.org)

N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. (biolegend.com)
E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. (qedbio.com)
A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. (ecmbio.com)
The cadherin (Ca ++ -dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. (bio-techne.com)
In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. (bio-techne.com)
Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. (enquirebio.com)
Consistent with the fact that higher level of δEF1 expression is associated with more invasive phenotype of breast cancer cells, our collective data suggests that δEF1 is likely the switch through which BMP-6 restores E-cadherin-mediated cell-to-cell adhesion and prevents breast cancer metastasis. (biomedcentral.com)
cadherin 2) are the most prominent members of the cadherin family of cell adhesion molecules. (biomedcentral.com)
Classical cadherins are cell adhesion molecules, which play fundamental roles in the development of multicellular organisms [ 1 ]. (biomedcentral.com)
E-cadherin is a calcium dependent adhesion molecule with important roles in epithelial intercellular adhesion and cellular structure. (cdc.gov)
Amagai M, Klaus-Kovtun V, Stanley JR. Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion. (medscape.com)

E-cadherin synthetic peptide corresponding to amino acids in the C-terminal region in human E-cadherin. (ecmbio.com)

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. (ecmbio.com)
This antibody was affinity purified using E-cadherin (a.a. 774-786) peptide. (ecmbio.com)

The mAb 8C11 recognizes the amino acids 92-593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. (biolegend.com)

Cadherins play a role in development, specifically in tissue formation. (rndsystems.com)
en Ksp-Cadherin (MRQ-33) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. (roche.com)
Variations in N-Cadherin / Cadherin-2 / CD325 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. (enquirebio.com)
Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. (rndsystems.com)
Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. (novusbio.com)
E-cadherin could be demonstrated in formalin or paraformaldehyde fixed tissues but not in glutaraldehyde fixed tissue. (cdc.gov)

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948 ). (rndsystems.com)

Interestingly, ectopic expression of δEF1 was able to block BMP-6-induced transactivation of E-cadherin, whereas RNA interference-mediated down-regulation of endogenous δEF1 in breast cancer cells abolished E-cadherin transactivation by BMP-6. (biomedcentral.com)

Anti-cadherin antibodies that block theformation of adherens junctions, for example, also block the formation of tightjunctions.Desmosomes Connect Intermediate Filaments from Cell toCellDesmosomes are buttonlike points of intercellular contact that rivet cellsTogether. (ostatic.com)
Autoantigens for IgA anti-intercellular antibodies of intercellular IgA vesiculopustular dermatosis. (medscape.com)

When compared with invasive ductal carcinoma, E-cadherin expression is markedly reduced or absent in the great majority of invasive lobular carcinomas when studied by immunohistochemistry. (qedbio.com)
The immunohistochemistry technique was performed by the estreptovidine biotine method using E-cadherin as the primary antibody. (bvsalud.org)

Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)
The aim of this study was to determine the evolution of E-cadherin expression in no special type (NST) primary breast carcinoma and to correlate this with that in distant, paired nodal metastases (LNM) and molecular classification. (iiarjournals.org)
E-Cadherin from primary breast cancer correlated positively only with E-cadherin in LNM (p≤0.003). (iiarjournals.org)
E-Cadherin expression in primary sites positively correlates with E-cadherin from LNM. (iiarjournals.org)

In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) N-Cadherin, recombinant mouse (rm) VE-Cadherin, rhCadherin-8, or rhCadherin-17 is observed. (rndsystems.com)

Additional reported applications (for the relevant formats) include: immunofluorescence 1,3,6 , motility inhibition of N-cadherin-expressing cells 2 , and Western blot 2,4 . (biolegend.com)
Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP2-27194] - IF Confocal analysis of A549 cells using ID2 antibody (NBP2-27194, 1:5). (novusbio.com)
The use of e-cadherin immunofluorescence in pulmonary toxicologic pathology studies. (cdc.gov)
Thus, in the lung, e-cadherin immunofluorescence can demonstrate abnormal and normal epithelial cell junctions, facilitate demonstration of airway epithelial changes, and distinguish podoplaninexpressing alveolar type I cells from lymphatic endothelium. (cdc.gov)

A specific band was detected for Serum Amyloid A1/A2 at approximately 14 kDa (as indicated) using 50 µg/mL of (Catalog # AF2948) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109 ). (rndsystems.com)

They can also exhibit antibodies to antigens associated with pemphigus vulgaris (desmoglein 3, 130-kd) and pemphigus foliaceus (desmoglein 1, 160-kd), as well as several others, including epiplakin. (medscape.com)

Serum and salivary IgG and IgA antibodies to desmoglein 3 in mucosal pemphigus vulgaris. (medscape.com)

The monoclonal antibody 2F4 was produced against the cytoplasmic domain (Red). (elifesciences.org)

Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. (rndsystems.com)
Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. (qedbio.com)
Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. (enquirebio.com)
Binding of KLRG1 to E-, N-, or R- cadherins blocks phosphorylation of Akt and increases the expression of cell cycle inhibitors. (biolegend.com)
Last but not least antibodies-online can provide customization with regards to expression system , tag location and sequence . (antibodies-online.com)
Conclusion: E-Cadherin expression is not stable during the metastatic process. (iiarjournals.org)
An immunohistochemical technique utilised the monoclonal antibody HECD-1 to evaluate E-cad expression in microwave treated, paraffin embedded sections from 120 patients. (nih.gov)
Quantitative RT-PCR was used to determine the expression of BMP-6, E-cadherin, and δEF1 at the mRNA level in MCF-7 and MDA-MB-231 breast cancer cells, as well as in 16 breast cancer specimens. (biomedcentral.com)
Further, we found that BMP-6 inhibited δEF1 transcription, resulting in an up-regulation of E-cadherin mRNA expression. (biomedcentral.com)
In addition to down-regulating the expression of δEF1, BMP-6 also physically dislodged δEF1 from E-cadherin promoter to allow the activation of E-cadherin transcription. (biomedcentral.com)
Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. (novusbio.com)
Mouse monoclonal anti-e-cadherin antibodies (BD Biosciences, San Jose, CA) in conjunction with fluorochrome-conjugated donkey anti-mouse antibodies clearly delineated sites of e-cadherin expression in lungs of rats or mice. (cdc.gov)
Low levels of e-cadherin expression in type I cells allowed the double labeled type I cells to be distinguished from lymphatic endothelium and facilitated diagnosis of lymphangiectasia. (cdc.gov)

N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. (biolegend.com)
We were unable to detect N-Cadherin / Cadherin-2 / CD325 in other tissues. (enquirebio.com)
In this regard E-Cadherin (E-cad) is a 120 kDa polypeptide present in all epithelial tissues and it is the prime mediator of cell-cell interactions. (nih.gov)

In contrast, MDA-MB-231 cells, an ER - cell line had significantly reduced BMP-6 and E-cadherin mRNA levels, suggesting an inverse correlation between BMP-6/E-cadherin and δEF1. (biomedcentral.com)

The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. (ecmbio.com)

Anti-CDH2 antibody staining is expected to be primarily localized to the cell junctions and plasma membrane. (enquirebio.com)
N-cadherin (N-cad, Cdh2) is initially expressed at the gastrulation stage when epiblast cells downregulate E-cad and undergo an epithelial-mesenchymal transition (EMT). (biomedcentral.com)

Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. (ecmbio.com)
Phosphorylation of Tyr-860 (Tyr-835 in E-cadherin) can disrupt cadherin binding to β-catenin. (ecmbio.com)
Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function. (ecmbio.com)

When clustered, VE‑Cadherin enhances T betaRII/T betaRI assembly into an active receptor complex on endothelial cells (12). (bio-techne.com)
Incubation of Cry1Ab protoxin with a single chain antibody that mimics the cadherin-like receptor and treatment with Manduca sexta midgut juice or trypsin, resulted in toxin preparations with high pore-forming activity in vitro. (unboundmedicine.com)

These antibodies bindto and disrupt the desmosomes that hold their skin epithelial cells(keratinocytes) together. (ostatic.com)

Low, but measureable presence of N-Cadherin / Cadherin-2 / CD325 could be seen in cells in the granular layer in cerebellum, glandular cells in the endometrium, stomach and thyroid gland and islets of Langerhans in pancreas. (enquirebio.com)

Western blot image of human A431 cells that were probed with rabbit polyclonal anti-E-Cadherin (a.a. 774-786) at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000 (lane 3) or mouse monoclonal anti-E-cadherin (Cytoplasmic) at 1:250 (lane 4) and 1:1000 (lane 5). (ecmbio.com)
This sequence is conserved in rat and mouse E-cadherin, and has low homology to other cadherin family members. (ecmbio.com)
Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. (bio-techne.com)
We recently addressed the question of interchangeability of the two cadherins by generating a mouse model in which N-cad cDNA was introduced into the E-cad locus. (biomedcentral.com)

Anatomy31

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Classical cadherin3

Immunogen2

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Proteins6

Member of the cadherin2

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Human E-cadherin1

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Pemphigus vulgaris1

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