Antibody binding. Medical search. Frequent questions

Here we report two monoclonal antibodies (mAbs) cloned from memory B cells of patients recently recovered from COVID-19, and both mAbs specifically bind to the spike (S) protein of SARS-CoV-2, block the binding of receptor binding domain (RBD) of SARS-CoV-2 to human angiotensin converting enzyme 2 (hACE2), and effectively neutralize S protein-pseudotyped virus infection. (medrxiv.org)
In this research project, we have investigated the C-terminal structures of small G proteins, purified the regulatory proteins for small G proteins, and made monoclonal antibodies against small G proteins and their regulatory proteins. (nii.ac.jp)
Among these regulatory proteins, we have cloned the cDNAs of smg p21 GDS, smg p25A GDI, and rho GDI, and made monoclonal antibodies against them. (nii.ac.jp)
Identification and Characterization of Functional Human Monoclonal Antibodies to Plasmodium vivax Duffy-Binding Protein. (tropmedres.ac)
Here, we describe a deep mutational scanning method to map how all amino-acid mutations in the RBD affect antibody binding and apply this method to 10 human monoclonal antibodies. (pacb.com)
This presentation will not include any discussion of the unlabeled use of a product or a product under investigational use except parts of the presentation will focus on monoclonal antibodies that are not FDA approved but are FDA authorized under emergency use authorizations EUA. (cdc.gov)
The US Food and Drug Administration has recently issued the emergency use authorization or EUA for monoclonal antibodies for both the treatment of COVID-19 and for post-exposure prophylaxis for certain patients, including patients with immunocompromising conditions. (cdc.gov)

A small number of H-bonds between peptide and antibody contribute to the affinity and specificity. (elsevierpure.com)
This great diversity and specificity is cause of diversity in Antigen Binding Site of heavy chain and light chain of antibody. (stackexchange.com)
Sensitivity and specificity with blocking antibody level together is 99.6%, and hence they are the tests of choice. (medscape.com)

Western blot analysis of extracts from 293 cells transfected with Bad or Bad Ser112 and/or Ser136 mutant fusion proteins, using Phospho-(Ser) 14-3-3 Binding Motif Antibody (top), Phospho-Bad (Ser136) Antibody #9295 (middle) or Phospho-Bad (Ser112) Antibody #9291 (bottom). (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated 14-3-3 binding motifs, using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
Antibody-antigen affinity is often evaluated with isolated proteins. (lu.se)
Cardiac myosin binding protein-C (cMyBP-C) is one of the proteins that make up the contractile apparatus of cardiomyocytes. (scirp.org)
There is a superfamily of ras p21/ras p21-like small GTP-binding proteins (small G proteins) with GTPase activity. (nii.ac.jp)
Small G proteins have two interconvertible forms, GDP-bound inactive and GTP-bound active forms. (nii.ac.jp)
In this thesis we have produced proteins that are densely decorated with carbohydrate determinants in order to study the glycosylation capacity of cell lines (paper I) and generate efficient binders of antibodies (paper II), bacterial toxins (paper III) and virus receptors such as the influenza hemagglutinin (paper IV). (avhandlingar.se)
Antibodies are heavy (~150 k Da ) proteins of about 10 nm in size, [7] arranged in three globular regions that roughly form a Y shape. (wikipedia.org)
Anti-dsDNA antibodies might also be created secondary to the production of antibodies to other proteins within the nucleosome. (wikipedia.org)
The presence of auto-antibodies that target synaptic machinery proteins was documented recently in immune-mediated cerebellar ataxias. (springer.com)
Among these diverse features, it should be acknowledged that auto-antibodies toward glutamate receptors, GABA receptors, and K + channel-related proteins are preferentially found in autoimmune limbic encephalitis but not in IMCAs [ 10 ] (Table 1 ). (springer.com)

This protocol outlines a high throughput assay for detecting anti-SARS-CoV-2 antibodies in patient serum samples, which is critical given the current pandemic. (jove.com)
To facilitate prospective surveillance for such evolution, we map how convalescent serum antibodies are impacted by all mutations to the spike's receptor-binding domain (RBD), the main target of serum neutralizing activity. (biorxiv.org)
Binding by polyclonal serum antibodies is affected by mutations in three main epitopes in the RBD, but there is substantial variation in the impact of mutations both among individuals and within the same individual over time. (biorxiv.org)
It is noteworthy that all of them share some escape mutations already known to have emerged under the selection pressure of antibody exposure, both from immune serum or therapeutic antibody use in prolonged survival within immunocompromised individuals, and from the antibodies elicited by natural infection. (news-medical.net)
Antibody tests can detect the presence of these antibodies in serum within days to weeks following acute infection or vaccination. (cdc.gov)
Mouse anti Human sex hormone binding globulin antibody, clone 30.6H2 does not react with human serum albumin. (bio-rad-antibodies.com)
The Igg Antibodies Binding Sites reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bioinfolab.org)
Description: A competitive ELISA for quantitative measurement of Monkey Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (bioinfolab.org)
it occurs when mannose-binding lectin (MBL), a serum protein, binds to mannose, fucose, or N -acetylglucosamine groups on bacterial cell walls, yeast walls, or viruses. (msdmanuals.com)
1] Therefore the reflex panel detects anti-acetylcholine receptor (blocking and binding) antibodies in the serum, if antibody level is greater than 0.4nmol/L, or antibody level is greater than 15% then modulating antibody is added. (medscape.com)
The rest might have anti-smooth muscle/anti-RyR/titin antibodies in the serum. (medscape.com)

The antibody is a rabbit polyclonal antibody raised against FKBP5. (uscnk.com)

Targeting specific protein-protein interactions (PPIs) is an attractive concept for drug development, but hard to implement since intracellular antibodies do not penetrate cells and most small-molecule drugs are considered unsuitable for PPI inhibition. (rcsb.org)
Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein-protein interaction is the binding affinity. (lu.se)
Determining the affinity of an antibody binding to its antigen is the first step in predicting the function in a physiological environment where other competing protein interactions may be present. (lu.se)
By MALDI-TOF mass spectrometry, we identified this protein to be cMyBP-C. These data were confirmed by immunostaining using the p-PKD-Ser744/748 antibody, which displayed a striated pattern similar to the one observ ed for a regular cMyBP-C antibody. (scirp.org)
Barefield, D. and Sadayappan, S. (2010) Phosphorylation and function of cardiac myosin-binding protein c in health and disease. (scirp.org)
Schlossarek, S., Mearini, G. and Carrier, L. (2011) Cardiac myosin-binding protein c in hypertrophic cardiomyopathy: Mechanisms and therapeutic opportunities. (scirp.org)
The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies. (jove.com)
The cryo-EM structure of SW186 bound to the receptor-binding domain (RBD) of the viral spike protein showed that SW186 interacted with an epitope of the RBD that is not at the interface of its binding to the ACE2 receptor but highly conserved among SARS coronaviruses. (flutrackers.com)
We have found that the C-terminal systeine residues of smg p21B, rhoA p21, and smg p25A are geranylgeranylated, and that these prenylation are essential for each small G protein to bind to membranes. (nii.ac.jp)
Carbohydrate - protein inte ractions in nature are often mediated via multivalent binding where the combined strength of multiple receptor - ligand interactions results in a binding that is highly specific and strong. (avhandlingar.se)
Magnetic Luminex Assay Kit for FK506 Binding Protein 5 (FKBP5) ,etc. (uscnk.com)
Engagement of the highly polymorphic domain II of the P. vivax Duffy-binding protein (DBPII) with the erythrocyte's Duffy Ag receptor for chemokines (DARC) is essential. (tropmedres.ac)
An antibody ( Ab ), also known as an immunoglobulin ( Ig ), [1] is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses . (wikipedia.org)
Product Description google Mouse anti-Calbindin-binding protein Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen. (biosensis.com)
Methyl-CpG binding protein 2 (Rabbit anti-MeCP2, R-1810-100, green) is universally expressed in the nuclei of almost all neurons. (biosensis.com)
Head-to-head comparison of available commercial antibodies against RNA-binding protein TIA1 by immunoblot (Western blot), immunoprecipitation and immunofluorescence. (openaire.eu)
TRP1 is a telomeric repeat binding protein with a DNA binding domain at its C terminus. (phytoab.com)
CBP20 is a nuclear cap-binding protein that forms a heterodimeric complex with ABH1 (AT2G13540) and is likely to participate in RNA metabolism. (phytoab.com)
Recombinant monoclonal antibody to Factor H binding protein. (absoluteantibody.com)
FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). (absoluteantibody.com)
The original Fab version of this antibody binds N. meningitidis fHbp protein with a very high binding affinity of Kd= 19pM. (absoluteantibody.com)
Functional Analysis of the Human Antibody Response to Meningococcal Factor H Binding Protein. (absoluteantibody.com)
The MUC-1 arm of BSA01 binds to the cleaved MUC1-C*protein, which remains membrane bound on tumor cells. (bmj.com)
Mouse anti Human sex hormone binding globulin antibody, clone 30.6H2 recognizes sex hormone binding globulin, also known as Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin or Testosterone-estrogen-binding globulin. (bio-rad-antibodies.com)
It is an androgen transport protein which binds and regulates the free concentration of steroid hormones and is involved in steroid signaling at the cell membrane. (bio-rad-antibodies.com)
The ChAdOx1-S [recombinant] vaccine uses a DNA adenovirus vector to elicit antibodies to the SARS-CoV-2 spike protein. (who.int)

In this chapter, we present a flow cytometry-based assay to measure and calculate the cell surface binding affinity or avidity of any mono- or polyclonal antibody solution. (lu.se)
Kumra Ahnlide, V & Nordenfelt, P 2023, Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry . (lu.se)
Flow cytometry was used to identify binding of this antibody to surface of live bacteria of N. meningitidis strains serogroup B strain H44/76, which expresses FHbp ID 1 (PMID: 26106082). (absoluteantibody.com)
Binding avidity was assessed by flow cytometry. (bmj.com)

Designing immunogens that elicit broadly neutralizing antibodies to conserved viral epitopes on the major surface glycoprotein, spike, such as the receptor binding domain (RBD) is one potential approach. (biorxiv.org)
Importantly, the cross-neutralizing antibody responses are focused towards conserved RBD epitopes outside of the ACE-2 receptor-binding motif. (biorxiv.org)
Eleven mAbs competed with each other for binding, indicating recognition of the same or overlapping epitopes. (tropmedres.ac)
The escape mutations cluster on several surfaces of the RBD that broadly correspond to structurally defined antibody epitopes. (pacb.com)
Epstein-Barr virus is also known to induce dsDNA antibodies, as seen after immunisation of animals with EBNA-1 epitopes. (wikipedia.org)

For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. (cellsignal.com)
Please refer to primary antibody product webpage for recommended antibody dilution. (cellsignal.com)

Erythrocyte-binding antigens of Plasmodium falciparum are targets of human inhibitory antibodies and function to evade naturally acquired immunity. (edu.au)
The evolution of SARS-CoV-2 could impair recognition of the virus by human antibody-mediated immunity. (biorxiv.org)
Our study illustrates the utility of epitope profiling to investigate the genetics of pathogen immunity, reports heritable features of the antibody response to viruses, and identifies specific HLA loci important for EBV epitope selection. (kcl.ac.uk)
After an antigen binds to a BCR, the B cell activates to proliferate and differentiate into either plasma cells , which secrete soluble antibodies with the same paratope, or memory B cells , which survive in the body to enable long-lasting immunity to the antigen. (wikipedia.org)
Because these fluids were traditionally known as humors , antibody-mediated immunity is sometimes known as, or considered a part of, humoral immunity . (wikipedia.org)
Moved "Development of Antibodies and Immunity" section. (cdc.gov)
Antibody tests have public health value for monitoring and evaluating population levels of immunity, as well as clinical utility for patients. (cdc.gov)
Antibody testing is not currently recommended to assess for immunity to SARS-CoV-2 following COVID-19 vaccination or to assess the need for vaccination in an unvaccinated person. (cdc.gov)
Antibody tests are not recommended or authorized by the FDA to assess someone's immunity after COVID-19 vaccination or determine if they need to be vaccinated. (cdc.gov)
Evidence has demonstrated sustained vaccine efficacy after a single 0.5ml dose for a period of up to 12 weeks (3 months), yet antibody concentrations declined by 34% through 90 days .2 Limited data is available on the duration of efficacy or rapidly waning immunity past 12 weeks, and a second dose has been shown to maintain high efficacy. (who.int)

Presence of other antibodies is highly variable, so these antibodies are not confirmatory for MG. Rather, their presence supports the diagnosis of MG. Also seronegative MG patients have a different presentation with presence of these antibodies. (medscape.com)

The invention relates to methods of modulating the antigen-binding affinity of an antibody by determining, using data corresponding to the structure of a complex between the antibody and an antigen in a solvent, a representation of a charge distribution of the CDRs of the antibody which minimizes electrostatic contribution to binding free energy between the antibody and the antigen in a solvent. (justia.com)

Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti -infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)

It is informative to also be able to assess antibody binding to a bacterial surface where many antigens might be present, including multiple copies of the specific antigen the antibody recognizes, and in a context where the antigen might be in a more natural conformation. (lu.se)

This antibody is broadly cross reactive and binds FHbp sequence variants from groups 1, 2, and 3. (absoluteantibody.com)
Upon exposure to pneumococcal polysaccharides, cross reactive antibodies between dsDNA and pneumococcal polysaccharides are produced in lupus. (wikipedia.org)

Antibodies targeting the SARS-CoV-2 spike receptor-binding domain (RBD) are being developed as therapeutics and are a major contributor to neutralizing antibody responses elicited by infection. (pacb.com)
Therefore, complete escape-mutation maps enable rational design of antibody therapeutics and assessment of the antigenic consequences of viral evolution. (pacb.com)

A potential solution to these problems is to select intracellular antibody fragments to block PPIs, use these antibody fragments for target validation in disease models and finally derive small molecules overlapping the antibody-binding site. (rcsb.org)
Our results may aid RAS-dependent cancer drug development and demonstrate a general concept for developing small compounds to replace intracellular antibody fragments, enabling rational drug development to target validated PPIs. (rcsb.org)
Structurally an antibody is also partitioned into two antigen-binding fragments (Fab), containing one V L , V H , C L , and C H 1 domain each, as well as the crystallisable fragment (Fc), forming the trunk of the Y shape. (wikipedia.org)
Other fragments and chimeric antibodies have also been made. (justia.com)
The natively paired VH and VL genes were expressed in Escherichia coli to obtain recombinant antibody fragments (Fabs). (absoluteantibody.com)
Essentially, the genes encoding the B-cell Receptor, or BCR, have many fragments of several different types in them, and through the process of gene rearrangement the final Complementarity Determining Regions, or CDRs (the antigen-binding sites), are generated into one mature sequence for each individual B-cell. (stackexchange.com)

6FA4: Antibody derived (Abd-7) small molecule binding to KRAS. (rcsb.org)
Here, we explore this strategy using an anti-mutant RAS antibody fragment as a competitor in a small-molecule library screen for identifying RAS-binding compounds. (rcsb.org)
The antibody recognizes a unique molecule of the pathogen, called an antigen . (wikipedia.org)

MBL = mannose-binding lectin. (msdmanuals.com)

Antibodies, which are also commonly referred to as immunoglobulins, contain four polypeptides: two longer polypeptides ("heavy chains") that are identical to one another and two shorter polypeptides ("light chains") that are identical to one another. (justia.com)
Antibody molecules or immunoglobulins (Ig) consist of heavy and light chains (e.g. two of each in IgG). (stackexchange.com)

Igg Antibody Laboratories manufactures the igg antibodies binding sites reagents distributed by Genprice. (bioinfolab.org)

We used VirScan, a high-throughput epitope scanning technology, to analyze pan-viral antibody reactivity profiles of twins and SNP-genotyped individuals. (kcl.ac.uk)
Simultaneous kinetic analysis of 384 antigen/antibody binding interactions using high throughput SPR. (carterra-bio.com)
High throughput kinetics facilitates robust selection of candidate antibodies with binding profiles better aligned to specific therapeutic objectives. (carterra-bio.com)

It recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ABH1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. (phytoab.com)

IA columns are used to remove anti - A and anti - B reactive antibodies prior to organ transplantation across the blood group ABO barrier. (avhandlingar.se)

We set out to examine whether the increased levels of rat-specific IgG and IgG 4 antibodies that we have previously observed at high allergen exposure in our cohort of laboratory animal workers play a functional role through blockage of the binding of IgE-allergen complex binding to CD23 receptors on B cells. (bmj.com)
Initially, all antibodies are of the first form, attached to the surface of a B cell - these are then referred to as B-cell receptors (BCR). (wikipedia.org)
The terms antibody and immunoglobulin are often used interchangeably, [1] though the term 'antibody' is sometimes reserved for the secreted, soluble form, i.e. excluding B-cell receptors. (wikipedia.org)
Anti-acetylcholine receptor antibody is found in 70-90% of patients with generalized acquired myasthenia gravis (MG). Lambert-Eaton syndrome is a close differential, as less than 13% of patients have clinical presentation similar to MG and antibodies against acetylcholine receptors in high titers. (medscape.com)
Binding antibody is the most common antibody found in patients with MG. These antibodies bind to the AChR on the muscle end plate and activate endocytosis/complement system leading to degradation of these receptors which results in poor muscle strength. (medscape.com)

Despite this inter- and intra-person heterogeneity, the mutations that most reduce antibody binding usually occur at just a few sites in the RBD's receptor binding motif. (biorxiv.org)
These show combinations of mutations in the spike region, including the receptor-binding domain (RBD) and N-terminal domain (NTD), as well as other genomic regions. (news-medical.net)
The mutations in both the B.1.351 and P.1 strains, including E484K and K417N/T, are thought to enhance viral fitness and may confer antibody resistance in part, at least. (news-medical.net)
However, even antibodies targeting the same surface often have distinct escape mutations. (pacb.com)
The complete escape maps predict which mutations are selected during viral growth in the presence of single antibodies. (pacb.com)
They further enable the design of escape-resistant antibody cocktails-including cocktails of antibodies that compete for binding to the same RBD surface but have different escape mutations. (pacb.com)
Antibodies elicited by infection accumulate somatic mutations in germinal centers that can increase affinity for cognate antigens. (nih.gov)

To overcome limitations of current EGFR- and MUC-1-targeting therapies, we generated a novel bispecific antibody-drug conjugate conjugated with monomethyl auristatin E, BSA01, which targets both antigens. (bmj.com)

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. (openaire.eu)

A more accurate depiction of an antibody (3D structure at RCSB PDB ). (wikipedia.org)

These results demonstrated that SW186 neutralizes diverse SARS coronaviruses by binding to a conserved RBD epitope, which could serve as a target for further antibody development. (flutrackers.com)
Using these data, we determined the heritability and genomic loci associated with antibody epitope selection, response breadth, and control of Epstein-Barr virus (EBV) viral load. (kcl.ac.uk)
[2] [3] Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (analogous to a key) on an antigen, allowing these two structures to bind together with precision. (wikipedia.org)
This antibody binds a surface exposed epitope on N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. (absoluteantibody.com)
Poor steric complementarity between PA1 and the antibody heavy chain along with the fact that the majority of the interactions between 2H1 and PA1 involve van der Waals interactions with the light chain may explain why this peptide acts as only a partial mimotope of the capsular polysaccharide epitope. (elsevierpure.com)

Right: Western blot analysis of calbindin expression (antibody dilution: 1:2,000) in rat cerebellum (2), pig hippocampus (3), and cow cerebellum (4). (biosensis.com)
Please also provide information about species, application, dilution and obtained result for the reviewed antibody. (agrisera.com)

Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes), using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
Phospho-(Ser) 14-3-3 Binding Motif Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (cellsignal.com)
Antibody-recruiting molecules (ARMs) are bispecific molecules composed of an antibody-binding motif and a target-binding motif that redirect endogenous antibodies to target cells to elicit immune responses. (rsc.org)
The peptide assumes a tightly coiled conformation consisting of one inverse γ-turn and one type II β-turn that serves to place the entire peptide motif, consisting of Thr(P5) Pro(P6), Trp(P8) Met(P9) and Leu(P10), into a depression in the antibody combining site. (elsevierpure.com)

Top) The results from a single capture kinetics assay where a monovalent target (analyte) was titrated over 384 antibodies, each captured onto individual spots of a 384-array. (carterra-bio.com)
In sandwich ELISA , the binding of ricin to ASF was dramatically decreased when galactose was present during the analyte or detection antibody binding step. (bvsalud.org)
The amount of the bound secondary antibody is directly proportional to the concentration of the analyte in the sample. (cdc.gov)

Both the on-rate ( k a ) and off-rate ( k d ) of antibodies are of equal importance in determining antibody affinity and potency. (carterra-bio.com)
In addition to increased affinity and neutralization potency, antibody evolution changed the mutational pathways for the acquisition of viral resistance and restricted neutralization escape options. (nih.gov)

My lab has been working in COVID-19 research, and we have recently developed a novel assay that allowed us to quickly and accurately detect SARS-CoV-2 antibodies. (jove.com)
Methods Parental MUC1 antibody was tested in a binding competition assay alongside a benchmark identifying cleaved MUC-1. (bmj.com)
Blood tests such as enzyme-linked immunosorbent assay (ELISA) and immunofluorescence are routinely performed to detect anti-dsDNA antibodies in diagnostic laboratories. (wikipedia.org)
Milk matrix effects on antibody binding analyzed by enzyme-linked immunosorbent assay and biolayer interferometry. (bvsalud.org)

To allow the immune system to recognize millions of different antigens, the antigen-binding sites at both tips of the antibody come in an equally wide variety. (wikipedia.org)

In an ELISA, this antibody shows concentration dependent binding to recombinant FHbp (ID 1), which is the variant group 1 antigen (PMID: 26106082). (absoluteantibody.com)
ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)

This can induce the antibody-mediated adverse drug effect of heparin-induced thrombocytopenia (HIT). (fu-berlin.de)

Here, we synthesized a novel Fc-binding ARM (Fc-ARM) that targets folate receptor (FR)-positive cancer cells, Reo-3, using a recently developed monocyclic peptide 15-Lys8Leu, which binds strongly to the Fc region of an antibody. (rsc.org)
This was found to confer a transmission advantage by stabilizing the conformation of the spike towards a state favorable for binding the host angiotensin-converting enzyme 2 (ACE2) receptor. (news-medical.net)
We analyzed 6 independent groups of clonally related severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) Spike receptor-binding domain (RBD)-specific antibodies from 5 individuals shortly after infection and later in convalescence to determine the impact of maturation over months. (nih.gov)
Normally, no acetylcholine receptor (AChR) antibody exists in the bloodstream. (medscape.com)
More than 30% of patients with MG are seronegative (ie, anti-acetylcholine receptor antibody is absent in these patients). (medscape.com)
These antibodies bind to the AChR on the muscle end plate and impairs binding of Ach with the receptor, which results in poor muscle strength. (medscape.com)

To enhance the translational potential of ARMs, it is crucial to design antibody/target-binding motifs that have strong affinity and are easy to synthesize. (rsc.org)
All mAbs were P. vivax strain transcending and targeted known binding motifs of DBPII with DARC. (tropmedres.ac)

Low concentrations of milk (1%, v/v) produced a similar reduction in ricin binding to ASF but not to a high-affinity monoclonal antibody (mAb), increasing the dissociation rate of ASF- ricin complexes up to 100-fold. (bvsalud.org)

GAD65 is involved in the synthesis, packaging, and release of GABA, whereas the other three play important roles in the induction of long-term depression (LTD). Thus, the auto-antibodies toward these synaptic molecules likely impair fundamental synaptic machineries involved in unique functions of the cerebellum, potentially leading to the development of cerebellar ataxias (CAs). (springer.com)

When a person becomes infected with a pathogen, their immune system makes antibodies specifically to fight it. (cdc.gov)

Antibodies from different classes also differ in where they are released in the body and at what stage of an immune response. (wikipedia.org)
Accordingly, there remains a need for antibodies that do not provoke a strong immune response but yet bind strongly to their antigens and methods for identifying such antibodies. (justia.com)
Antibodies are just one part of a persons' immune response. (cdc.gov)
Although the exact mechanism of the generation of dsDNA antibodies is still unknown, it is likely that extracellular DNA is one cause of an immune response against dsDNA. (wikipedia.org)

The measurement of antibodies binding to IFNbeta in MS patients treated with IFNbeta. (ox.ac.uk)

Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize it directly (for example, by blocking a part of a virus that is essential for its invasion). (wikipedia.org)
1] Antibodies are positive in 90% of patients, but 10% patients have no detectable levels of binding/blocking/modulating antibodies. (medscape.com)
Blocking antibody is the second most common antibody found in MG patients. (medscape.com)

Erythrocyte-binding antigens of Plasmodium falciparum are targets of human inhibitory antibodies and. (edu.au)
In this study, we used novel approaches to determine the importance of P. falciparum erythrocyte-binding Ags (EBAs), which are important invasion ligands, as targets of human invasion-inhibitory Abs and define their role in contributing to immune evasion through variation in function. (edu.au)
Anti-GAD65 antibody (Ab) acts on the terminals of inhibitory neurons that suppress GABA release, whereas anti-VGCC, anti-mGluR1, and anti-GluR Abs impair LTD induction. (springer.com)

We identified 12 DBPII-specific human mAbs from distinct lineages that blocked DBPII-DARC binding. (tropmedres.ac)
Researchers hoped that such antibodies would be no more foreign to the human body than a genuine human antibody, but the utility of such antibodies has been restricted, at least in some cases, by a reduction in the antibody's affinity for the antigen. (justia.com)
The affinity of the anti-MUC1 antibody was similar to human and cynomolgus monkey antigens. (bmj.com)
Purified sex hormone binding globulin detected with Mouse anti Human Sex Hormone Binding Globulin ( MCA5812G ) followed by HRP conjugated Rabbit F(ab') 2 anti Mouse IgG ( STAR13B ). (bio-rad-antibodies.com)

Secondary antibodies labeled with conventional organic dyes (Alexa Fluor) are used as reporters. (cdc.gov)

In conclusion, PSGL - 1/mIgG 2b carrying specific carbohydrates is a versatile tool that can be used in a range of applications where the multivalency confers a biologically relevan t binding. (avhandlingar.se)

Biacore biosensor assays indicated that PSGL - 1/mIgG 2b carrying the blood group P1 determinant in multiple copies bound with high affinity to Stx1 and Stx2, while PSGL - 1/mIgG 2b presenting multiple copies of Sia α 2,3Gal on different O - linked cores bound with high affinity to avian influenza H5. (avhandlingar.se)
This makes it a high priority that standardized assays using authentic virus be used in addition to pseudoviruses, to measure the sensitivity of the virus to antibodies. (news-medical.net)
Results Binding competition assays indicate that the parental MUC1 antibody of BSA01 binds to MUC1-C*, which remains membrane-bound after cleavage. (bmj.com)

Blebs on apoptotic cells contain nearly all the autoantigens found in SLE, and phagocytes bind these apoptotic cells and phagocytose them. (wikipedia.org)

Here, we used the LIBRA-seq technology, which identified SARS-CoV-2 specific B cells via DNA-barcoding and subsequently single cell sequenced BCRs, to identify an antibody, SW186, which could neutralize major SARS-CoV-2 variants of concern, including Beta, Delta, and Omicron, as well as SARS-CoV-1. (flutrackers.com)
For certain antibodies, affinity maturation enabled the neutralization of circulating SARS-CoV-2 variants of concern and heterologous sarbecoviruses. (nih.gov)

Conjugation service and the corresponding secondary antibody are available. (biomatik.com)

Anatomy23

Organisms19

Diseases6

Chemicals and Drugs116

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Phenomena and Processes49

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science5

Health Care5

Monoclonal antibodies7

Specificity3

Proteins11

Serum11

Rabbit polyclonal antibody1

Protein26

Flow Cytometry4

Epitopes5

Primary antibody2

Immunity10

Presence of these antibodies1

Affinity of an antibody1

Antigens and Antibodies1

Specific antigen1

Cross reactive2

Therapeutics2

Fragments6

Molecule3

Mannose-Bindin1

Immunoglobulins2

Reagents1

High throughput3

Recognizes1

Reactive antibodies1

Receptors5

Mutations7

Conjugate1

Characterization1

RCSB PDB1

Epitope5

Dilution2

Motif4

Analyte3

Potency2

Assay4

Antigen-bindi1

ELISA2

Induce1

Receptor6

Motifs2

Complexes1

Molecules1

Pathogen1

Immune response4

Measurement1

Blocking3

Inhibitory3

Human4

Dyes1

Confers1

Assays3

Apoptotic cells1

Variants of concern2

Secondary1