• In the context of recent evidence for a putative proto-germinal center in fishes we propose some possible reasons that observed affinity maturation in fishes often seems lacking and propose future work that might shed further light on this process in fishes. (mdpi.com)
  • The presence of specific antibodies in vaccinated mice serum was studied by immunoblot analysis at different days after vaccination and showed an intensive immune response to a wide range of antigens with molecular weight ranging between 22 and 250 kDa. (hindawi.com)
  • some antibodies are expected to bind light chain sites common to all immunoglobulins. (mpbio.com)
  • Prestige Antibodies® have been used for subcellular localization studies by immunocytochemistry (ICC) - immunofluorescence (IF) using the protocol described below. (sigmaaldrich.com)
  • To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . (sigmaaldrich.com)
  • antibody - An immunoglobulin molecule produced by B lymphoid cells with a specific amino acid sequence evoked in humans or other animals by an antigen (immunogen). (academic.ru)
  • Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) and buffer salts. (mpbio.com)
  • Prestige Antibodies® recommended for Western Blot are recommended to be used according to the standard WB protocol described below. (sigmaaldrich.com)
  • Proper validation with controls and optimization ensure the quality of the antibody, and establish confidence in your results. (abgent.com)
  • To distinguish between these possibilities, we immunized wild-type and Apobec3 -deficient C57BL/6 (B6) mice with (4-hydroxy-3-nitrophenyl) acetyl (NP) hapten and evaluated the binding affinity of the resultant NP-specific Abs. (jimmunol.org)
  • These studies revealed similar affinity maturation of NP-specific IgG1 Abs between wild-type and Apobec3 -deficient mice in the absence of FV infection. (jimmunol.org)
  • In contrast, hapten-specific Ab affinity maturation was significantly compromised in Apobec3 -deficient mice infected with FV. (jimmunol.org)
  • Median affinities of sera from children 2-6 (1.5 x 10(5) l/mol) and 7-11 months of age (1.6 x 10(5) l/mol) were significantly greater than the median affinities of sera from infants 12-18 (1.8 x 10(4) l/mol) or greater than 18 months of age (4.2 x 10(4) l/mol). (nih.gov)
  • GANP can augment the induction of somatic hypermutation (SHM) in the V regions, resulting in the affinity maturation of V regions during the proliferation and differentiation of Ag-driven B cells in GCs. (genscript.com)
  • Quality is embedded in the production process to assure the best antibody for you. (abgent.com)