Antibody adenovirus e1a proteins. Medical search. Frequent questions

The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
The antibodies may be delivered by two approaches, either encoded within the OV genome to restrict antibody production to sites of active virus infection or alternatively given alongside OVs as separate treatments. (bmj.com)
cis versus trans combination Cis combination involves the 'arming' of transgenes that encode antibodies or any anticancer agents in the oncolytic virus (OV) genome. (bmj.com)
Schematic of the adenovirus genome and adenovirus-based vectors. (genemedi.com)
Top panel: a simplified map of the adenovirus serotype 5 genome showing the early genes (E1-E4) and the region from which the major late transcript is produced. (genemedi.com)
Without integration into host genome, adenovirus genome remains in an episomal state, which guarantees the low risk of mutation (Fig. 8). (genemedi.com)
The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can't be integrated into host genome, guaranteeing the security for subsequent operations. (9lb.cc)

The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
Since the adenoviral E1A gene has been shown to partially transform some epithelial cells from primary rat cell cultures, we constructed retrovirus vectors containing either the 12S or 13S E1A cDNA sequences to facilitate the transfer of these genes into a variety of primary cell types. (cshl.edu)
The 12S E1A virus induced proliferation and immortalization of epithelial cells in rat kidney, liver, heart, pancreas, and thyroid primary cultures. (cshl.edu)
53:742-750, 1985), in which the 12S E1A protein is required for the complete induction of the cellular DNA replication machinery in the quiescent human epithelial cells in which adenoviruses normally replicate. (cshl.edu)
The neutralizing antibody response to PC-positive DBs was superior to that of PC-negative DBs, as tested on fibroblasts, epithelial cells, and endothelial cells and for both animal species used. (asm.org)
Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
Bronchial epithelial cells (BEAS-2B cells and primary bronchial epithelial cells) were exposed to heat-inactivated Haemophilus influenzae , Pseudomonas aeruginosa or Streptococcus pneumoniae and subsequently infected with respiratory syncytial virus (RSV), type 2 human adenovirus or influenza B. Levels of pro-inflammatory cytokines, viral replication and expression of pattern recognition receptors were determined in culture supernatants and/or cell lysates. (biomedcentral.com)

These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
Systemic administration is necessary for the treatment of metastatic disease, however the intravenous administration of adenovirus results in viral retention in the liver causing significant infection of hepatocytes leading to liver damage [ 1 ]. (oncotarget.com)
Of particular interest was the finding that PC-positive DBs induced an antibody response that blocked the infection of fibroblasts by a PC-positive viral strain more efficiently than sera following immunizations with PC-negative particles. (asm.org)
Using a subviral particle vaccine candidate, we show here that one protein complex of HCMV, termed the pentameric complex (PC), enhances the neutralizing antibody response against viral infection of different cell types. (asm.org)
the presence of the PC in the particles also enhanced the neutralizing antibody response against the infection of fibroblasts by HCMV. (asm.org)
The high prevalence of HAdVs and the neutralizing antibodies associated with prior infection, may limit HAdV-based vector use in human subjects. (bvsalud.org)
The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (ersjournals.com)
These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
In the present study, the hypothesis that adenoviral infection followed by persistent expression of its E1A gene is capable of producing epithelial-mesenchymal transformation was examined by investigating guinea pig PLECs that express adenoviral E1A. (ersjournals.com)
For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR), which mainly determines the viral tropism [44]. (genemedi.com)
Infection process of adenovirus. (genemedi.com)
to determine the average age of ms onset vs the age at which epstein-barr infection has previously occurred and stratify this analysis by sex and the blood level of epstein-barr nuclear antigen 1 (ebna1) antibody. (liverpool.ac.uk)
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. (hindawi.com)
Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. (hindawi.com)
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children [ 1 , 2 ]. (hindawi.com)
Recombinant protein expression of interest is detectable from 24 hours post infection. (9lb.cc)

The Rb protein regulates differentiation, apoptosis, and cell cycle control by coordinating the cell cycle at G1-S with transcriptional machinery. (affbiotech.com)
Together, our findings show that adenoviruses coding for CD40L mediate multiple antitumor effects including oncolysis, apoptosis, induction of T-cell responses, and upregulation of T(H)1 cytokines. (mek-inhibitors.com)
Using a cell apoptosis assay based on stimulation of a RANK:Fas fusion protein, and a cellular NF-kappaB signaling assay, we showed that R12-31 was agonist for both species. (cnrs.fr)

The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. (9lb.cc)
Genemedi has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need. (9lb.cc)

Moreover, the long-term systemic delivery of a recombinant protein is an expensive, painful experience for patients and is cumbersome for medical staff. (biomedcentral.com)
Generally, pathogen products used in subunit vaccines include purified or recombinant protein-inactivated bacterial toxins, or polysaccharides present on surface capsules. (medicaltrend.org)
Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. (9lb.cc)

Oncolytic adenoviruses hold great promise for cancer treatment since they can replicate and destroy cancer cells. (oncotarget.com)
The development of more potent oncolytic adenoviruses faces with a potential increase in associated-toxicities. (oncotarget.com)
To improve the safety of oncolytic adenoviruses a control of the viral tropism based on tissue-specific miRNA regulation has been exploited. (oncotarget.com)
Antiviral immune responses present a major hurdle to the efficacious use of oncolytic adenoviruses as cancer treatments. (aacrjournals.org)
In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell-engager (BiTE) antibodies. (aacrjournals.org)

In this work, we have studied the feasibility of providing tissue-specific selectivity to replication-competent adenoviruses through the regulation of the late structural protein fiber (L5 gene). (oncotarget.com)
The microvessel density and Ad replication in tumor tissue were evaluated by checking the expression of CD34 and hexon proteins, respectively. (biomedcentral.com)
The transgenic expression of Ad5 E1A and E1B19K simulated the rescue effect of dl 1520 supporting Ad-Endo replication in GC cells. (biomedcentral.com)
One-step growth curve showed that the replication of the SAdVs with HAdV-5 E4orf6 substitution and E1/E3 deletion was similar to that of wild-type SAdVs in HEK293 cells, but the modified SAdVs were replication-deficient in A549 cells which lack HAdV-5 E1A and E1B. (bvsalud.org)
Adenovirus is often used as a viral vector to produce vaccines with replication ability and vaccines with replication defects. (medicaltrend.org)
In order to produce a replication-deficient vector, the two early genes E1A and E1B of adenovirus were replaced with transgene (the insertion antigen of the target pathogen) expression cassette, thereby preventing the virus from replicating in the host cell. (medicaltrend.org)
Oncolytic adenovirus buy NU7441 is an attractive platform for immunotherapy because virus replication is highly immunogenic and not subject to tolerance. (mek-inhibitors.com)
Adenovirus transcription is a two-phase event, early and late, occurring before and after viral DNA replication, respectively (Figure. (genemedi.com)
Life cycle of adenovirus is separated by DNA replication process into two distinct phases: the early and late, occurring before and after viral DNA replication, respectively. (genemedi.com)
To prevent infected cell lysis, recombinant replication deficiency virus has been developed as a gene delivery tool to replace wild-type adenovirus (recombinant AdV in "application" part). (genemedi.com)
During Adv DNA replication, host cellular proteins such as nuclear factor I and POU2F1 are used by Adv [ 13 , 14 ]. (hindawi.com)
Simply, the E1 gene products are early proteins that are transcribed in the early transcribed regions and required for proceeding subsequent steps in viral replication. (9lb.cc)
The E1 gene contains E1A and E1B, involved in the replication of adenovirus. (9lb.cc)
E1A is critical to start viral replication by promoting transcription from rep gene promoters, P5 and P19, and facilitate viral replication by activating the early adenovirus promoters. (9lb.cc)

E1A exerts this change in the cell cycle by interacting with the transcriptional machinery of the cell. (bdbiosciences.com)
In vitro studies indicate that Sur-2 interacts with the E1A zinc finger protein and the Srb/Mediator transcriptional multiprotein complex. (bdbiosciences.com)
However, the roles of Sur-2 in regulating the transcriptional activity of the Srb/Mediator complex and E1A remain to be fully elucidated. (bdbiosciences.com)

Possessing over60 serotypes, adenovirus is recognized as the etiologic agent of various diverse syndromes. (medscape.com)
In humans, 57 adenovirus serotypes have been identified and further divided into 7 subgroups (A-G). Most adenovirus-based vaccines are prepared using adenovirus type 5 (Ad5). (medicaltrend.org)

Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
Both human and non-human simian adenoviruses (HAdVs and SAdVs, respectively) have been used as gene therapy and vaccine vectors. (bvsalud.org)
Transduction results of various Adenovirus vectors. (9lb.cc)

Monoclonal antibodies specific for simian virus 40 tumor antigens. (wikipedia.org)
Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.cn)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)

In addition, by genetically depleting the E3 and E4 genes of the adenovirus vector, the host cell-mediated elimination of adenovirus-infected cells and the instability of the transgene can be prevented. (medicaltrend.org)
The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. (9lb.cc)

A family of human cdc2-related protein kinases. (wikipedia.org)
Immunocytochemistry/ Immunofluorescence: MORF4L2 Antibody [NBP2-47370] - Staining of human cell line A-431 shows localization to nucleoplasm. (novusbio.com)
Immunohistochemistry-Paraffin: MORF4L2 Antibody [NBP2-47370] - Staining of human colon tissue. (novusbio.com)
Western Blot: MORF4L2 Antibody [NBP2-47370] -Analysis in human cell line MCF-7. (novusbio.com)
Immunohistochemistry-Paraffin: MORF4L2 Antibody [NBP2-47370] - Staining of human cerebral cortex shows strong nuclear positivity in neurons. (novusbio.com)
The human homolog of SUR-2 (hSUR-2) was shown to interact with the E1A zinc finger domain from adenovirus. (bdbiosciences.com)
The antibody recognizes human Sur-2. (bdbiosciences.com)
An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
Gene therapy using a recombinant adenovirus (Ad) encoding secretory human endostatin (Ad-Endo) has been demonstrated to be a promising antiangiogenesis and antitumor strategy of in animal models and clinical trials. (biomedcentral.com)
Human adenoviruses (HAdVs) contain linear, double-stranded DNA with an average genomic length of 35 kbp. (cdc.gov)
This review will address epigenetic regulation in members of the polyomaviruses, adenoviruses, human papillomaviruses, hepatitis B, and herpes viruses. (mdpi.com)
In this study, we constructed a novel oncolytic adenovirus, Ad5/3-hTERT-E1A-hCD40L, which features a chimeric Ad5/3 capsid for enhanced tumor RG-7112 cost transduction, a human telomerase reverse transcriptase (hTERT) promoter for tumor selectivity, and human CD40L for increased efficacy. (mek-inhibitors.com)
We found that both antibodies recognized mouse RANK with high affinity, while RANK-02 and R12-31 recognized human RANK with high and lower affinities, respectively. (cnrs.fr)
Both antibodies recognized human Langerhans cells, previously shown to express RANK, while dermal dendritic cells were poorly labeled. (cnrs.fr)
Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [42]. (genemedi.com)

Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
These peptides were then tested in our previously described oncolytic cancer vaccine platform PeptiCRAd, a vaccine platform that combines an immunogenic oncolytic adenovirus (OAd) coated with tumor antigen peptides. (elifesciences.org)

Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. (lookformedical.com)

Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. (lookformedical.com)
Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. (lookformedical.com)

Among Harlow's discoveries was the demonstration that the retinoblastoma protein interacts with viral transforming proteins, thereby linking tumor viruses with the cell cycle. (wikipedia.org)
The NuA4 complex interacts with MYC and the adenovirus E1A protein. (novusbio.com)

Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
Here, we characterize such a protein, iASPP (inhibitory member of the ASPP family), encoded by PPP1R13L in humans and ape-1 in Caenorhabditis elegans. (ox.ac.uk)

Further, in particular embodiments, the chimeric virus has a different immunological profile than one or both of the parent viruses (i.e., is only weakly or not at all recognized by neutralizing antisera or antibodies against the parent virus), thereby allowing for administration to subjects that have antibodies directed against the parent virus or repeat administration following administration of another serotype. (justia.com)
Oncolytic viruses and antibodies: are they more successful when delivered separately or when engineered as a single agent? (bmj.com)
His early research includes seminal work done in 1989, demonstrating the importance of cell cycle proteins in the functioning of DNA tumor viruses. (newswise.com)
The transforming gene products of these viruses, such as the E1A oncoproteins of adenovirus 5, led to the identification of cellular factor p60, known as cyclin A. This research was the first demonstration of a physical link between cellular transformation and the cell cycle, thereby paving the way for the melding of these two areas of research. (newswise.com)

In the two cases tested, heart and liver cultures, E1A-immortalized cells were nontumorigenic, but could be completely transformed by subsequent introduction of the ras oncogene. (cshl.edu)
Hence, an inhibitory form of ASPP resembling 53BP2 could allow cells to bypass the tumor-suppressor functions of p53 and the ASPP proteins. (ox.ac.uk)
Moreover, iASPP is an oncoprotein that cooperates with Ras, E1A and E7, but not mutant p53, to transform cells in vitro. (ox.ac.uk)
Anti-TNF-α antibodies are in animals very effective in protecting the retinal cells and the optic nerve-and might therefore be useful prophylactically against secondary glaucoma in future such patients. (bvsalud.org)
Actin antibody: The specific visualization of actin filaments in non-muscle cells. (cshlpress.com)
Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. (cshlpress.com)
Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. (aacrjournals.org)
Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)
Regulation of antibody secretion by hybridoma cells. (cornell.edu)
Mechanism of idiotype-induced suppression of antibody secretion by hybridoma cells. (cornell.edu)
Through immunoprecipitation of YY1-bound chromatin from affected individuals' cells with antibodies recognizing both ends of the protein, we show that YY1 deletions and missense mutations lead to a global loss of YY1 binding with a preferential retention at high-occupancy sites. (unimi.it)
In normal cells the BRCA1 protein is localized in the nucleus, whereas in the majority of breast cancer cell lines and in malignant pleural effusions from breast cancer patients, it is localized mainly in the cytoplasm. (lookformedical.com)
In addition, non-replicating mRNAs can be used to produce RNA-related vaccines that involve mRNA sequences encoding pathogen-specific proteins (antigens) in host cells. (medicaltrend.org)
In this review, we provide a brief summary of the combination OV-antibody therapies that target tumor cells, tumor microenvironment and immune cells to help define key parameters influencing which approach is superior, thereby improving insight into the rational design of OV treatment strategies. (bmj.com)
The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. (cancerindex.org)
Adv infects the host cells through binding to different cellular receptors such as coxsackievirus and adenovirus receptor (CAR) [ 12 ]. (hindawi.com)
Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues. (9lb.cc)
Selected results of Adenovirus transduction in the adult rat cardiac cells (A), eyes (B), neuron cells (C). (9lb.cc)

Physical mapping of temperature-sensitive mutations of adenoviruses. (cshlpress.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

Such vaccines do not include other protein sequences that induce self-amplification of the inserted mRNA sequence. (medicaltrend.org)
Adenovirus-based vaccines can induce antibody-mediated and T-cell- mediated immune responses. (medicaltrend.org)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)

1985. Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element. . (cornell.edu)

The adenovirus E1A protein can bind to a number of cellular proteins to alter cell cycle progression. (bdbiosciences.com)

This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. (novusbio.com)
Blobel GA. CREB-binding protein and p300: molecular integrators of hematopoietic transcription. (bdbiosciences.com)
Varied poly(A) site efficiency in the adenovirus major late transcription unit. (cornell.edu)
1991. Sequences regulating temporal poly(A) site switching in the adenovirus major late transcription unit. . (cornell.edu)

It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA. (ersjournals.com)

The combination of OVs with antibodies has been widely explored as a strategy to achieve synergistic antitumor activity. (bmj.com)

Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)
A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
Recombinant vaccinia virus and adenovirus have been developed as potent vaccine vehicles for treating cancer and infectious diseases. (dukecancerinstitute.org)
The viral pentameric protein complex consisting of glycoprotein H (gH)/gL/UL128-131A (PC) is considered to be an important vaccine component. (asm.org)

CAR receptor-fiber-knob adenovirus interaction and internalization process [46]. (genemedi.com)
Antibody-AdV complexes can provoke inflammatory cytokine and chemokine responses in macrophages via the intracellular antibody receptor TRIM21 [56-59]. (genemedi.com)

Conjugate vaccines are made by combining polysaccharide residues in the bacterial surface capsule with carrier proteins (such as diphtheria toxoid protein). (medicaltrend.org)

Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. (wikipedia.org)
Besides binding to coagulation proteins, AdV in blood vessel can also bind to component C3 and natural [51-55]. (genemedi.com)

Use as little as 2 ml cell media to isolate high yield of exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins. (reportergene.com)

This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc. (bdbiosciences.com)
WB: For western blot detection of denatured protein samples. (affbiotech.com)

These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia. (hindawi.com)

A range of antibody formats has been considered, including whole antibody molecules of different isotypes, fragments such as F(ab), single-chain variable fragment (scFv) and nanobodies and bispecific and trispecific agents that can mediate a variety of functions such as T-cell activation or binding and inactivation of pro-tumorigenic cytokines. (bmj.com)

These studies have generated adenoviruses with miRNA target sites placed in the 3'UTR of early gene products. (oncotarget.com)
In vivo , liver production of infective viral particles was highly impaired, similarly to that triggered by an adenovirus with miRNA target sites regulating the early E1A gene. (oncotarget.com)
Thus, miRNA-control of late proteins constitutes a novel strategy to provide selectivity to adenoviruses. (oncotarget.com)
The top 3 upregulated miRNA (hsa-miR-127-3p, hsa-miR-493-5p, and hsa-miR-409-3p) were identified in adenovirus-infected children and provided a clear distinction between infected and healthy individuals. (hindawi.com)

E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin. (ersjournals.com)
In RNA vaccines, mRNA is used as a template for the endogenous synthesis of the target pathogenic protein (antigen). (medicaltrend.org)

volume = {171}, number = {1879-0542 (Electronic)}, pages = {5--14}, abstract = {Antibodies play an important role in therapy and investigative biomedical research. (cnrs.fr)

Quantitative determination of adenovirus-mediated gene delivery to rat cardiac myocytes in vitro and in vivo. (cornell.edu)

MORF4L1 may also participate in the formation of NuA4 related complexes which lack the HTATIP/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. (novusbio.com)
Both exhibited cuboidal morphology and junctional complexes, but did not contain lamellar bodies or express surfactant protein A, B or C mRNAs. (ersjournals.com)

Hexon and penton structures form the capsid of AdV, and fiber protein mediates the binding of the virion to the cell surface and is a major determinant of viral tropism. (genemedi.com)
Then the endosome acidifies, resulting in disassociation of capsid proteins and transportation of viral DNA into nucleus. (genemedi.com)

Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. (lookformedical.com)

These models are being used to systematically elucidate proteins required for cutaneous carcinogenesis and to test their potential role as therapeutic targets. (stanford.edu)

These studies suggest that Sur-2 may play a role as a bridge between E1A and the Srb/Mediator multiprotein complex. (bdbiosciences.com)
Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein. (bdbiosciences.com)

Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. (thermofisher.com)
Regulation of poly(A) site selection in adenovirus. (cornell.edu)

The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. (wikipedia.org)
Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)

RB antibody detects endogenous levels of total RB. (affbiotech.com)

Jul 16, 2018 - Cladribine, EMA/OD/087/17 Recombinant monoclonal antibody to sialic acid-binding Ig-like lectin 8. (pdfkul.com)

The oncolytic adenovirus ICOVIR-15K was engineered to express an EGFR-targeting BiTE (cBiTE) antibody under the control of the major late promoter, leading to generation of ICOVIR-15K-cBiTE, which retained its oncolytic properties in vitro . (aacrjournals.org)

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