Antibody acceptor. Medical search. Frequent questions

Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. (bdbiosciences.com)
Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
The use of monoclonal antibodies is ubiquitous in science and biomedicine but the generation and validation process of antibodies is nevertheless complicated and time-consuming. (hereon.de)
The system has been validated for globular protein antigens as well as for haptens and enables a fast and early stage selection and validation of monoclonal antibodies in one step. (hereon.de)
Regular and long exposures revealed the overall similarity of the 3-band profiles in T1 and T2, but resPrP D profile, including glycoform representation, differed in the 2 phenotypes with all 3 monoclonal antibodies (Ab) used. (cdc.gov)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. (avivasysbio.com)
Specificity analysis using Rabbit Anti-ALK (pTyr1507) Polyclonal Antibody (SPC-913). (2bscientific.com)
Primary Antibody: Rabbit Anti-ALK (pTyr1507) Polyclonal Antibody (SPC-913) at 1:1000. (2bscientific.com)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
The invention relates to methods of modulating the antigen-binding affinity of an antibody by determining, using data corresponding to the structure of a complex between the antibody and an antigen in a solvent, a representation of a charge distribution of the CDRs of the antibody which minimizes electrostatic contribution to binding free energy between the antibody and the antigen in a solvent. (justia.com)
Guided by these determinations, the antibody is accordingly modified (altered) to improve upon, e.g., antibody/antigen binding by modifying at least one amino acid residue to decrease the binding free energy between the antibody and antigen when bound in a solvent. (justia.com)
Researchers hoped that such antibodies would be no more foreign to the human body than a genuine human antibody, but the utility of such antibodies has been restricted, at least in some cases, by a reduction in the antibody's affinity for the antigen. (justia.com)
Specific site on an antigen to which an antibody binds. (ebi.ac.uk)
His Monoclonal antibody research is multidisciplinary, relying on both Immunofluorescence, Combinatorial chemistry, Antigen and Dissociation constant. (research.com)
To address these issues we developed a novel selective technology based on an artificial cell surface construct by which secreted antibodies were connected to the corresponding hybridoma cell when they possess the desired antigen-specificity. (hereon.de)

Buccutite™ APC-iFluor® 700 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. (aatbio.com)
Among them AAT Bioquest's Buccutite™ crosslinking technology offer the most efficient method to conjugate antibodies with macromolecules such as enzyme tags. (stratech.co.uk)

The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)
Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)
Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. (avivasysbio.com)
As a control, the expression levels of enzyme-fused protein and target proteins were detected using each protein-specific antibody (left panel). (elifesciences.org)
AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC-iFluor® 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. (aatbio.com)
Our kit provides preactivated APC-iFluor® 700 tandem to facilitate the APC-iFluor® 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. (aatbio.com)
In addition, our preactivated APC-iFluor® 700 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies. (aatbio.com)
Immunoblot analysis of immunoprecipitated GLUT4 with antibodies that specifically recognize ubiquitin (or an epitope-tagged version of ubiquitin) revealed that the transporter is ubiquitinated (Figure 2A B). Ubiquitin is conjugated to proteins through an amide linkage between its carboxy-terminus and a primary amino group often contributed by a lysine residue on the target protein (18). (researchdataservice.com)
Figure 2 GLUT4 is ubiquitinated in yeast and its endosomal trafficking requires both ubiquitin-acceptor sites and GGA proteins. (researchdataservice.com)

The DREG-56 monoclonal antibody specifically binds to CD62L. (bdbiosciences.com)
The C-terminal half from the large chain particularly binds to extracellular acceptors at peripheral cholinergic nerve terminals [12] that innervate striated and even muscle tissues. (exposed-skin-care.net)

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody. (cellsignal.com)
Secondary Antibody: Anti-Rabbit IgG: HRP at 1:100000.WT Peptide Sequence: N-P-T-pY-G-S-W. SPOT peptide synthesis was used to perform substitution analysis of the phospho-acceptor AA in the sequence that was used to produce the phospho-site-specific antibody. (2bscientific.com)

NfsB is a dimeric flavoprotein enzyme that has broad electron acceptor specificity 15 and has been used in conjunction with prodrugs to selectively ablate cells in vertebrates. (cdc.gov)

There are many varieties of antibody conjugation technologies available and each has its benefits, though no single one is optimal for every payload and antibody. (stratech.co.uk)
To make sure that conjugation from the antibody towards the C2 cassette of GluN1 didn't adversely have an effect on data acquisition, we produced an CD221 additional build, GluN1*F554C-TS, to permit for attachment towards the ready coverslip a Twin-Strep-tag (find Online Strategies), generating equivalent results (Supplementary Outcomes, Supplementary Amount 1). (antiviralbiologic.com)

This antibody recognizes an epitope found between amino acids 216-375 in the DNA-binding domain of PARP. (thermofisher.com)
Both antibodies are highly specific for a distinct epitope on the protein. (cisbio.com)
For the design of the construct we combined the transmembrane domain of the EGF-receptor with a hemagglutinin epitope and a biotin acceptor peptide and performed a transposon-mediated transfection of myeloma cell lines. (hereon.de)

Green: IDH2 protein stained by IDH2 antibody diluted at 1:500. (novusbio.com)
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or transiently transfected with plasmid encoding Myc/DDK-tagged COX2/MT-CO2 protein (lane 2), using COX2/MT-CO2 Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
By Western blot, this antibody recognizes a 116 kDa protein which corresponds to PARP and the 85 kDa apoptosis-induced cleavage product of prICE and CPP32 from rat thymus extract. (thermofisher.com)
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. (thermofisher.com)
This antibody is purified through a protein A column, followed by peptide affinity purification. (avivasysbio.com)
The glucosylphosphotransferase acceptor in rat liver is also immunoprecipitated by the anti-parafusin antibody and is very similar in physical characteristics to the paramecium protein. (elsevierpure.com)
A phosphorylated cellular protein (analyte) is sandwiched between a streptavidin (SA)-coated Donor bead associated with an analyte antibody and a Protein A-conjugated Acceptor bead associated with an antiphospho specific antibody. (bmglabtech.com)
The first antibody was selected for its specific binding to the phosphorylated motif on the protein, and the second for its ability to recognize the protein independent of its phosphorylation state. (cisbio.net)
Protein phosphorylation enables an immune-complex formation involving both labeled antibodies and which brings the donor fluorophore into close proximity to the acceptor, thereby generating a FRET signal. (cisbio.net)
By EKB-569 expressing individual GLUT4 in we demonstrate it traffics towards the endosomal program in a fashion that depends on ubiquitin-acceptor sites and GGA protein. (researchdataservice.com)
Outcomes Trafficking of GLUT4 through fungus endosomes needs ubiquitin-acceptor sites and GGA protein Previous research expressing mammalian GLUT4 in reported which the transporter localizes towards the endoplasmic reticulum (ER) (10 11 We reasoned that ER retention could be due to the high degrees of appearance found in these research and therefore indicated GLUT4 through the regulatable promoter to accomplish lower degrees of manifestation. (researchdataservice.com)

Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). (bdbiosciences.com)
The 3-cube approach is based on measuring donor emission upon donor-specific excitation, acceptor emission upon acceptor-specific excitation and acceptor emission upon exciting the donor. (imagej.net)

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000. (novusbio.com)
Western blot analysis of extracts from HeLa cells, cultured in the absence (-) or presence of Ethidium Bromide (+), using COX2/MT-CO2 Antibody (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
Western blot analysis was performed using COX2/MT-CO2 Antibody. (cellsignal.com)
Western blot analysis of extracts from various cell lines using COX2/MT-CO2 Antibody (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)

His research integrates issues of Biochemistry, Monoclonal antibody, Polymerase chain reaction and Recombinant DNA in his study of Molecular biology. (research.com)

The Total RSK1 assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. (cisbio.net)
The Total IKK-beta assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. (cisbio.com)
In presence of IKKß in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. (cisbio.com)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
The antibodies were isolated by affinity chromatography by aprotinin immobilized on CNBr activated Sepharose. (uni-bielefeld.de)

For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. (cellsignal.com)

The AlphaScreen ® assay uses the diffusion of singlet state oxygen from Donor to Acceptor beads. (bmglabtech.com)

Please refer to primary antibody product webpage for recommended antibody dilution. (cellsignal.com)
Primary antibody ( ) incubation with antibodies against OAS1 ( CST ), RNase L (CST), or FLAG (Sigma) were performed in 5% milk in PBS-T overnight at 4°C. (bioz.com)

Several methods rely on measuring fluorescence intensities of the donor and acceptor to determine the extent of FRET from donor quenching and/or sensitized acceptor emission. (imagej.net)
Timo Lövgren mostly deals with Chromatography, Analytical chemistry, Monoclonal antibody, Europium and Fluorescence spectrometry. (research.com)

The following product was used in this experiment: IDH1 Monoclonal Antibody (OTI2H9) from Thermo Fisher Scientific, catalog # MA5-24966, RRID AB_2724497. (thermofisher.com)
The following product was used in this experiment: PARP1 Monoclonal Antibody (C-2-10) from Thermo Fisher Scientific, catalog # MA3-950, RRID AB_325523. (thermofisher.com)

Other fragments and chimeric antibodies have also been made. (justia.com)

It catalyses the transfer of ADP-ribose units from NAD (+) to a number of nuclear acceptor molecules including chromatin. (biogenex.com)

A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. (wikipedia.org)

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. (abcam.com)
Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is COX2/MT-CO2 Antibody. (cellsignal.com)
An immunoreactivity with bikunin and TFPI was not detected, Similar results were obtained with polyclonal anti-aprotinin antibodies from rabbit. (uni-bielefeld.de)
Rabbit Antibodies Against Bk Ca Channels, supplied by Alomone Labs, used in various techniques. (bioz.com)

Timo Lövgren combines subjects such as Detection limit, Analyte, Monoclonal antibody and Molecular biology with his study of Immunoassay. (research.com)

Many of these arrive at FRET indices which are semiquantitative, qualitative, or outright ignorant of actual donor-acceptor ratios. (imagej.net)
Properly describing the sources of the signal in each channel - taking spectral crosstalk, instrument sensitivity, and varying donor-acceptor ratio into account - allows for deriving a quantitatively correct expression for FRET efficiency. (imagej.net)

We detected anti-aprotinin antibodies by an enzyme immunoassay in serum of a 33 year old man who showed anaphylactic reactions during heart transplantation under aprotinin reexposition. (uni-bielefeld.de)

This antibody was developed for use in flow cytometry. (bdbiosciences.com)
The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. (aatbio.com)

Once internalized, BoNT is normally no longer vunerable to circulating neutralizing antibodies or various other impermeable inhibitors of its toxicity. (exposed-skin-care.net)

a) GluN1*F554C/GluN2A* NMDA receptors had been tagged with donor and acceptor fluorophores at site 554 of GluN1, proximal towards the initial transmembrane portion of GluN1 Amoxicillin Sodium (mean fluorophore positions proven as green or crimson hard spheres encircled with a fluorophore cloud, and C of F554 on GluN1 proven as an orange sphere). (antiviralbiologic.com)
smFRET discovered steady and distinctive state governments For smFRET tests, we portrayed GluN1*F554C/GluN2A* Amoxicillin Sodium receptors within a derivative of individual embryonic kidney cells (HEK293T cells), labelled them with acceptor and donor fluorophores, and immunopurified the tagged receptors on ready coverslips using an antibody toward the C-terminus (abcam ab64572). (antiviralbiologic.com)

Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. (novusbio.com)

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. (abcam.com)

Note: When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed. (bdbiosciences.com)
Antibody working concentrations are 0.6ug per ml. (avivasysbio.com)

Omicron mutations enhance infectivity and reduce antibody neutralization of SARS-CoV-2 virus-like particles ' by Syed, A., et al. (asm.org)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)

When a biomolecular interaction brings the Donor and Acceptor beads in proximity, the singlet oxygen reaches the Acceptor bead and a cascade of chemical reactions is initiated producing a greatly amplified luminescence signal in the range of 520-620 nm. (bmglabtech.com)
Methods: The amplified luminescent proximity homogeneous assay (AlphaLISA) method, which incorporates glutathione- or streptavidin-donor beads and anti-human-IgG-acceptor beads, was used to evaluate serum antibody levels in serum samples. (longdom.org)

On some antibodies, our APC-iFluor® 700 Tandem is much brighter than APC-Alexa Fluor® 700 Tandem with a higher stain index. (aatbio.com)
Values of using Alexa 488‐conjugated antibodies. (mdm2-inhibitors.com)
After Alexa 555 acceptor bleaching (bleached area in white circle) there was a 37? (mdm2-inhibitors.com)
To reduce nonspecific labelling by donor and acceptor fluorophores (Alexa 555 maleimide and Alexa 647 maleimide, respectively) we mutated the available cysteines Cys15 and Cys22 in GluN1 and Cys231, Cys399, and Cys460 in GluN2A to serines, using the causing background constructs known as GluN1* and GluN2A*19C22 hereafter. (antiviralbiologic.com)

They investigated the binding interaction for insulin and an insulin-specific antibody, in addition to developing the first radioimmunoassay (RIA) for insulin. (wikipedia.org)
Histograms show the level of SSTR2-specific antibody binding to wild-type Jurkat T cells (0%, top) and Jurkat T cells transduced with increasing virus titers. (jci.org)
B ) Level of SSTR2-specific antibody binding to SSTR2-transduced Jurkat T cells with and without preincubation with 1 μM octreotide (37°C, 30 minutes). (jci.org)
This antibody is specific for p85 cleaved form of PARP1. (abcam.com)
Dot blot analysis of Phospho-PARP1-S372 Antibody Phospho-specific Pab (Cat. (avivasysbio.com)
By loading Au nanoparticles onto rhombic dodecahedral ZIF-8, the substrate (Au/ZIF-8) has large specific surface area and can immobilize antibody (Ab1) by Au-N bonds. (bvsalud.org)

Note Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labeled well. (aatbio.com)

This PARP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S372 of human PARP1. (avivasysbio.com)

Antibodies, which are also commonly referred to as immunoglobulins, contain four polypeptides: two longer polypeptides ("heavy chains") that are identical to one another and two shorter polypeptides ("light chains") that are identical to one another. (justia.com)
Reasons behind its success was, there was no immunosuppression( a process to slow down the immunity level of acceptor body to welcome foreign antibodies) between genetically identical twins. (nationaldaystoday.com)

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)

When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)

Complementary analysis to engineer and develop high-affinity, monoclonal neutralizing antibodies can be being executed [11]. (exposed-skin-care.net)

Samples were probed with IDH1 (Product # MA5-24966) monoclonal antibody. (thermofisher.com)

Antibodies Against Oas1, supplied by Cell Signaling Technology Inc, used in various techniques. (bioz.com)

Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Mouse kidney [673] diluted at 1:500. (novusbio.com)

The antibody was conjugated with BD Horizon BUV737 under optimal conditions that minimize unconjugated dye and antibody. (bdbiosciences.com)

In this study, we aimed to identify additional antibody markers that would facilitate screening. (longdom.org)

Anatomy17

Organisms14

Diseases4

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Phenomena and Processes57

Disciplines and Occupations2

Information Science5

Health Care5

Monoclonal antibodies5

Polyclonal Antibody3

Antigen7

Method to conjugate antibodies2

Proteins9

Binds2

Anti-rabbit IgG2

Electron acceptor1

Conjugation2

Epitope3

Protein11

Excitation2

Western Blot4

Recombinant1

Donor fluorophore3

Affinity chromatography2

Membrane1

Assay uses1

Primary2

Fluorescence2

Thermo Fisher Sci2

Chimeric1

Chromatin1

Macromolecules1

Rabbit4

Analyte1

FRET2

Enzyme1

Flow cytometry2

Inhibitors1

Receptors2

Immunofluorescence1

Species1

Concentrations2

Mutations1

Isotype1

Beads2

Alexa4

Specific6

Serum1

PARP11

Identical2

Datasheet1

Spectral1

Analysis1

Product1

Cell1

Kidney1

Optimal1

Facilitate1