• The following product was used in this experiment: AKAP12 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-21759, RRID AB_11154150. (thermofisher.com)
  • The following product was used in this experiment: Phospho-RET (Tyr1016) Polyclonal Antibody from Thermo Fisher Scientific, catalog # 44-277G, RRID AB_2533620. (thermofisher.cn)
  • In prior work, we provided some immune system evasion strategies utilized by FIPV that could clarify Rabbit Polyclonal to Myb why antibodies appear to be unable to recognize contaminated cells and/or tag them for antibody-dependent cell lysis. (conferencedequebec.org)
  • The receptor tyrosine kinase RET is a member of the cadherin superfamily. (thermofisher.cn)
  • Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. (cancer.gov)
  • Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. (cancer.gov)
  • Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. (ningbobio.com)
  • c-Fgr is a human non-receptor tyrosine kinase family member that was discovered by using a probe toward the v-Fgr portion of the cell-derived domain of Gardner-Rasheed feline sarcoma virus. (ningbobio.com)
  • It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. (thermofisher.com)
  • CD90 (Thy-1) is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. (bdbiosciences.com)
  • The PrP-activated signal transduction pathway is associated with axon and dendritic outgrowth with a series of kinases. (biolegend.com)
  • Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
  • The ↑[Ca 2+ ] i activates a signaling cascade that involves, sequentially, calcium-calmodulin, nitric oxide synthase, guanylyl cyclase, and protein kinase G. This signal transduction pathway enables Cl − to pass through vesicular channels and act as a counterion for the transport of H + , so that the vesicles of secretogogue-stimulated cells become acidic. (jneurosci.org)
  • No. 559975) and PE mouse anti-rat CD90/mouse CD90.1 (clone OX-7) monoclonal antibodies. (bdbiosciences.com)
  • Small-molecule inhibitors, antagonistic monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), bispecific antibodies or biologics (bsAbs) and chimeric antigen receptor-modified T cells (CAR-Ts) targeting Notch signaling components have been developed as investigational anti-cancer drugs ( 10 - 12 ). (spandidos-publications.com)
  • Furthermore, inhibitory anti-DAF monoclonal antibodies reversed this response, while the inhibitory anti-CD59 did not, showing DAF inhibits C3 binding to HMECs. (biomedcentral.com)
  • The subject of the study was the major fibrous sheath protein of the mouse sperm flagellum, AKAP82, a member of the A Kinase Anchor Protein (AKAP) family of polypeptides that bind the regulatory (RII) subunit of protein kinase A (PK-A). Immunoelectron microscopy demonstrated that AKAP82 is present throughout the transverse ribs and longitudinal columns of the fibrous sheath. (elsevierpure.com)
  • AKAP8 Antibody detects endogenous levels of total AKAP8. (affbiotech.com)
  • Human peripheral blood lymphocytes were stained with purified anti-CD230 (Prion) antibody (clone 6D11, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE. (biolegend.com)
  • The immunogen of 15004-1-AP is TCTN1 Fusion Protein expressed in E. coli. (ptglab.com)
  • The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. (thermofisher.com)
  • GSK 3 is normally a portrayed, active constitutively, proline-directed serine/threonine kinase involved with diverse biophysiological features including glycogen fat burning capacity, embryo development, tissues injury, regeneration and repair, immunomodulation, and redox homeostasis16. (mingsheng88.org)
  • This protease activity has recently been ascribed to serine metalloprotease(s), including pregnancy-associated plasma protein-A (PAPP-A), which was first detected in human follicular fluid nearly 20 yr ago. (bioone.org)
  • Phospho-ABL1/2 (Tyr412) Antibody detects endogenous levels of ABL1/2 only when phosphorylated at Tyr393, which site historically referenced asTyr412. (affbiotech.cn)
  • Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
  • The underlying pathology is the autoimmune production of immunoglobulin G (IgG) antibodies directed toward receptors on the postsynaptic membrane at neuromuscular junctions (NMJs). (medscape.com)
  • Small‑molecule γ‑secretase inhibitors (AL101, MRK‑560, nirogacestat and others) and antibody‑based biologics targeting Notch ligands or receptors [ABT‑165, AMG 119, rovalpituzumab tesirine (Rova‑T) and others] have been developed as investigational drugs. (spandidos-publications.com)
  • A-type ephrins bind A-type receptors, which are tethered to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. (rndsystems.com)
  • The family of tyrosine-kinase receptors, including those for epithelial growth factor (EGFR) and the transporter for the neurotransmitter dopamine (DAT), are regulated in this way. (alzforum.org)
  • It is an autoimmune disorder, in which weakness is caused by circulating antibodies that block acetylcholine receptors at the post-synaptic neuromuscular junction, [1] inhibiting the stimulative effect of the neurotransmitter acetylcholine. (doctorbhatia.com)
  • Some forms of the antibody impair the ability of acetylcholine to bind to receptors. (doctorbhatia.com)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • We have engineered and examined several different constructs for pretargeting, beginning with bispecific diabodies that had monovalent binding to the target antigen and hapten, then triabodies with divalent binding to the tumor and monovalent binding to a hapten, and now a novel platform technology called the Dock-and-Lock (DNL) method for fusing different proteins recombinantly (Rossi et al. (radiologykey.com)
  • Indirect ELISA (ie, binding of Antibody to Antigen coated plate). (cancer.gov)
  • We after that continued to RG14620 elucidate by which internalisation pathway these antigen-antibody complexes are internalised. (conferencedequebec.org)
  • The following product was used in this experiment: ZO-1 Monoclonal Antibody (ZO1-1A12) from Thermo Fisher Scientific, catalog # 33-9100, RRID AB_2533147. (thermofisher.com)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15004-1-AP (TCTN1 Antibody) at dilution of 1:50 (under 40x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15004-1-AP (TCTN1 Antibody) at dilution of 1:50 (under 10x lens). (ptglab.com)
  • Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using TCTN1 antibody (15004-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptglab.com)
  • Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
  • Other clinical studies with an 131 I-labeled HP provided further evidence that pretargeting could deliver similar radioactivity to tumors compared to directly radiolabeled anti-CEA antibody, but with lower normal tissue accretion (Bardies et al. (radiologykey.com)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • In addition, CeD patients produce highly disease-specific antibodies against deamidated gluten peptides and the enzyme tissue transglutaminase 2 (TG2) ( 2 - 4 ). (frontiersin.org)
  • Breakdown of immune tolerance is believed to be one of the major mechanisms which triggers the production of autoantibodies by B cells and antibody forming cells, leading to inflammation upon binding to autoantigens and consequent tissue damage [ 2 ]. (hindawi.com)
  • In the discovery set of fresh-frozen tissue specimens (14), the A-kinase anchor protein 12 (AKAP12) protein was found downregulated across the grades. (nature.com)
  • There is also traffic in the opposite direction: molecules are transported into the cell from outside, including worn-out or excess receptor and channel proteins from the outside of the cell membrane. (alzforum.org)
  • Src-family ki-nases contain an amino-terminal cell membrane anchor followed by an SH3 domain and an SH2 domain, which are involved in modular association and activation, respectively. (ningbobio.com)
  • [15] PrP C attaches to the outer surface of the cell membrane by a glycosylphosphatidylinositol anchor at its C-terminal Ser 231. (wikidoc.org)
  • Bispecific antibodies based on the Dock-and-Lock procedure. (radiologykey.com)
  • AstraZeneca), patients randomly assigned to receive this combination of a third-generation TKI and an EGFR-MET bispecific antibody experienced a statistically significant, clinically meaningful improvement in progression-free survival, with overall survival also trending in favor of lazertinib-amivantamab. (aacrjournals.org)
  • There are also motor proteins, including both dynein and kinesin, which generate forces that move chromosomes during mitosis. (wikipedia.org)
  • Long-range transportation to get in the cell periphery towards the cell center works over microtubules and it is mediated with the electric motor proteins dynein and kinesin. (conferencedequebec.org)
  • Purified by protein-A affinity chromatography. (exbio.cz)
  • The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. (reliatech.de)
  • This has led to the development of a range of ERK1/2 inhibitors (ERKi) that either inhibit kinase catalytic activity (catERKi) or additionally prevent the activating pT-E-pY dual phosphorylation of ERK1/2 by MEK1/2 (dual-mechanism or dmERKi). (babraham.ac.uk)
  • moreover, PI3′-kinase inhibitors (wortmannin and LY294002) antagonized secretion. (jneurosci.org)
  • An anti-NgR1 monoclonal antibody 700000000000 blocks Nogo MAG and OMgp binding to NgR1 and successfully promotes neurite outgrowth from neurons cultured on CNS myelin substrates (Li (Zheng assays (e.g. soluble indigenous protein in development cone collapse assays versus dried out and partly denatured proteins in outgrowth assays). (bio-biz-navi.com)
  • Tau interacts with actin in the cytoskeleton and neuronal outgrowth, anchors enzymes such as protein kinases and phosphatases, and regulates intracellular vesicle transport. (biovendor.com)
  • The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.cn)
  • In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. (uiowa.edu)
  • Various lysates were subjected to SDS PAGE followed by western blot with 15004-1-AP (TCTN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Western blot was performed using Anti-ZO-1 Monoclonal Antibody (ZO1-1A12) (Product # 33-9100) and a 225 kDa band corresponding to ZO-1 was observed across along with uncharacterized band. (thermofisher.com)
  • WB: For western blot detection of denatured protein samples. (affbiotech.com)
  • In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)
  • In its inactive condition, Nrf2 is normally sequestered in the cytoplasm and from the actin anchored Kelch-like ECH-associated proteins 1 (Keap1)7,8. (mingsheng88.org)
  • Another dynamin-dependent nonclassical pathway is certainly actin and Rho-kinase reliant but indie of rafts and can be used by intracellular adhesion molecule-1 and platelet-endothelial cell adhesion molecule-1 [14]. (conferencedequebec.org)
  • ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factorα (TNFα), the p75 TNF receptor, the β-amyloid protein precursor, and the c-kit ligand1, implying that it may participate in shedding of these membrane proteins. (reliatech.de)
  • Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. (affbiotech.com)
  • 4. In silico prediction if the immunogenicity of selected peptides and assessing their source proteins. (helsinki.fi)
  • Many of these proteins are conserved between eukaryotic species, including a specialized histone H3 variant (called CENP-A or CenH3) which helps the kinetochore associate with DNA. (wikipedia.org)
  • The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. (ptglab.com)
  • The optimal Ig concentration for an application varies by species and antibody affinity. (uiowa.edu)
  • In line with a more complex picture, multiple isoforms of the protein likely exist and non-centrosomal sites of localisation have been reported. (springer.com)
  • The protein can exist in multiple isoforms , the normal PrP C , and as Protease resistant PrP Res like the disease-causing PrP Sc(scrapie) and an isoform located in mitochondria . (wikidoc.org)
  • The recombinant anti-tumor Fd naturally forms non-covalent dimers ( b ), with the resulting complex providing a "docking" site that binds to a 17-amino acid sequence derived from the A-kinase anchor proteins. (radiologykey.com)
  • The OX-7 monoclonal antibody specifically binds to rat CD90 reported to be expressed by hematopoietic stem cells, early myeloid and erythroid cells, immature B lymphocytes in the bone marrow and peripheral lymphoid organs, thymocytes, recent thymic emigrants (a subset of CD45RC- peripheral T lymphocytes), neurons, glomerular mesangial cells, endothelium at inflammatory sites, mast cells, and dendritic cells. (bdbiosciences.com)
  • DOTA antibody reporter 1 (DAbR1) comprises a single-chain fragment of the antilanthanoid-DOTA antibody 2D12.5/G54C fused to the human CD4-transmembrane domain and binds irreversibly to lanthanoid ( S )-2-(4-acrylamidobenzyl)-DOTA (AABD). (snmjournals.org)
  • The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. (cancer.gov)
  • In the nucleus, Nrf2 identifies and binds to a conserved antioxidant response component (ARE) and induces transcription of the battery pack of chemoprotective antoxidant genes9,10, including those encoding antioxidant proteins like heme oxygenase (HO-1)11. (mingsheng88.org)
  • This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant ADAM9 extracellular domain. (reliatech.de)
  • Parafollicular (PF) cells secrete 5-HT in response to stimulation of a G-protein-coupled Ca 2+ receptor (CaR) by increased extracellular Ca 2+ (↑[Ca 2+ ] e ). (jneurosci.org)
  • There are two structural groups of ephrin ligands which bind to a family of receptor tyrosine kinases (Eph). (rndsystems.com)
  • PF cells were found to express regulatory (p85) and catalytic (p110α and p110β) subunits of phosphatidylinositol 3′-kinase (PI3′-kinase). (jneurosci.org)
  • Antibodies are present at the neuromuscular junction (NMJ), the site of pathology. (kieran101.com)
  • It has been suggested that synemin could function as a linker between different cytoskeletal components based on the fact that it interacts with several proteins involved in the organization of the costameres, neuromuscular and myotendinous junctions within striated muscle cells. (biomedcentral.com)
  • The human c-Fgr gene encodes a 529 amino acid protein. (ningbobio.com)
  • PRNP ( PR io N P rotein) is the human gene encoding for the major prion protein PrP (for pr ion p rotein), also known as CD230 ( cluster of differentiation 230). (wikidoc.org)
  • Two Novel Variants in the Protein S Gene PROS1 Are Associated with Protein S Deficiency and Thrombophilia. (cdc.gov)
  • Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. (exbio.cz)
  • Alkaline phosphatase treatment of epididymal sperm proteins demonstrated that p109 was a phosphorylated form of pro-AKAP82 that remained in sperm. (elsevierpure.com)
  • Acetylcholine receptor (AChR) antibodies are detected in approximately 85% of patients with generalized myasthenia gravis, who are categorized as seropositive. (medscape.com)
  • [ 1 ] The membrane protein muscle-specific tyrosine kinase (MuSK) has been identified as the target of antibody attack in approximately 40% of patients with seronegative myasthenia gravis. (medscape.com)
  • Antibodies to this protein can be produced by patients with myasthenia gravis. (thermofisher.com)
  • Several lines of evidence suggest that myasthenia gravis (MG) is an organ-specific, antibody-mediated auto-immune disease. (kieran101.com)
  • Myasthenia gravis is an autoimmune disease: it features antibodies directed against the body's own proteins. (doctorbhatia.com)
  • Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Metturn expected for reprolysins, a disintegrin like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. (reliatech.de)
  • Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. (cancer.gov)
  • Prion protein contains 5 amino-terminal octapeptide repeats with sequence PHGGGWGQ. (wikidoc.org)
  • A glycosylphosphatidylinositol (GPI) membrane anchor at the COOH-terminal tethers PrP to cell membranes. (biolegend.com)
  • Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)
  • therefore, autoimmune attack by anti-MuSK antibodies is another mechanism through which interaction with acetylcholine on the postsynaptic surface is reduced. (medscape.com)
  • Another 3% to 7% of patients have antibodies directed against muscle tyrosine kinase (MuSK), another NMJ protein. (kieran101.com)
  • The cytoplasmic domain of ADAM9 interacts with Src homology 3 (SH3)containing proteins and protein kinase C, and may mediate different signaling pathways. (reliatech.de)
  • Of several of the pathways, glycogen synthase kinase (GSK) 3 provides surfaced as the integration stage and plays an essential function in managing the Nrf2 activity. (mingsheng88.org)
  • Canonical Notch signaling activates the transcription of BMI1 proto‑oncogene polycomb ring finger, cyclin D1, CD44, cyclin dependent kinase inhibitor 1A, hes family bHLH transcription factor 1, hes related family bHLH transcription factor with YRPW motif 1, MYC, NOTCH3, RE1 silencing transcription factor and transcription factor 7 in a cellular context‑dependent manner, while non‑canonical Notch signaling activates NF‑κB and Rac family small GTPase 1. (spandidos-publications.com)
  • Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 μg/ml, GAM APC). (exbio.cz)
  • Separation of CD48 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 μg/ml, GAM APC). (exbio.cz)
  • Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. (reliatech.de)
  • The assembly of the mammalian sperm flagellum is a complex developmental event requiring the sequential activation of genes encoding the component parts and the coordinated assembly of these proteins during the differentiation of the haploid spermatid. (elsevierpure.com)
  • In the cells that perform exhibit viral proteins, these proteins are internalised upon antibody addition through an extremely effective and fast procedure leading to FIPV-infected cells without aesthetically detectable viral proteins on the plasma membrane [3]. (conferencedequebec.org)
  • Tyrosine kinase that plays an essential role for the selection and maturation of developing t-cell in the thymus and in mature t-cell function. (affbiotech.cn)
  • Schwab and co-workers pioneered the delivery of anti-Nogo strategies by transplanting YIL 781 hybridoma cells improved to secrete the IN-1 monoclonal antibody aimed contrary to the amino-terminal of Nogo-A. IN-1-treated pets that underwent a dorsal over-hemisection damage exhibited significant axonal regeneration and useful recovery (Schnell & Schwab 1990). (bio-biz-navi.com)
  • In sperm, much of the RII also became particulate, consistent with the hypothesis that AKAP82 anchors RII in the flagellum. (elsevierpure.com)
  • We tested the hypothesis that protein kinase C (PKC) participates in stimulus-secretion coupling. (jneurosci.org)
  • Tau is a microtubule-associated protein found predominantly in neuronal axons of the vertebrate brain. (biovendor.com)
  • Membrane proteins from cultured embryonic Drosophila neuronal cells. (uiowa.edu)
  • DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. (snmjournals.org)
  • Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. (ningbobio.com)
  • Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). (springer.com)
  • The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. (biolegend.com)
  • Src-family kinases, which are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. (ningbobio.com)
  • Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization and membrane trafficking. (ningbobio.com)
  • We discovered that just half from the contaminated monocytes express viral proteins on the RG14620 surface area [2]. (conferencedequebec.org)
  • The 3rd dynamin-independent internalisation pathway was provided in our prior work and may be the pathway by which viral surface area portrayed proteins in FIPV contaminated monocytes are internalised. (conferencedequebec.org)
  • 2012. Prevalence of antibodies to bovine viral diarrhoea virus in bulk tank milk and associated risk factors in Scottish dairy herds . (hutton.ac.uk)
  • The bsMAb studied mostly consisted of a murine anti-CEA Fab' conjugated chemically to the Fab' of a murine antibody that bound indium-loaded DTPA (Le Doussal et al. (radiologykey.com)
  • These initial studies of bsMAb pretargeting were limited because the bsMAb was of murine origin, and patients developed an anti-mouse antibody response. (radiologykey.com)
  • We then began a collaboration with these French investigators, introducing a Fab' x Fab' bsMAb having a humanized anti-CEA coupled to the murine anti-DTPA antibody (Kraeber-Bodere et al. (radiologykey.com)
  • In contrast to prior studies with the murine bsMAb, where most patients developed an anti-mouse antibody response, only one of 12 patients developed an anti-mouse response, but four developed antibody responses to the humanized anti-CEA portion of the bsMAb. (radiologykey.com)
  • However, these antibodies cannot block infection, which implies that antibodies and antibody-driven immune system effectors cannot efficiently clear your body from pathogen and/or virus-infected cells. (conferencedequebec.org)
  • Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)