Monoclonal antibodiesImmunogenPeptideSpecificitySecondary antibodiesRabbitDetects endogenous levelsEndogenousProteinsDatasheetAntigenReactivityFlow CytometryCustom antibody servicesDilutionCloneThermo Fisher SciNeuroscienceProtein PhosphorylationSignal TransductionIsoformLanesReagentsRecombinantTarget antibodyPhosphopeptideChromatographyPeptidesHumanKinasesLysatesMembranePrimaryLysateImmunofluorescenceGlycerolMouseInhibitorDescriptorModificationsAssays
Monoclonal antibodies6
- We also identify rabbit monoclonal antibodies displaying broad specificity (MJFF-pRAB8) that can be utilised to assess LRRK2-con-trolled phosphorylation of a range of endogenous Rab proteins, including Rab8A, Rab10 and Rab35. (dundee.ac.uk)
- Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
- UW-Madison researchers have developed three novel monoclonal antibodies that all have exceptional activity against human and mouse GATA2. (warf.org)
- Monoclonal antibodies are laboratory-produced proteins that target specific antigens on the cancer cell's surface to interfere with the cell's function and destroy it. (lls.org)
- Some monoclonal antibodies are combined with a toxin or radioactive substance. (lls.org)
- One approach involves the generation of isoform- and phospho-specific polyclonal and monoclonal antibodies to identify the cellular and subcellular sites of expression of ion exchangers in the kidney and other tissues, and to study their regulation. (yale.edu)
Immunogen2
- Antibodies directed against immunogen-coupled phosphorylated PEPTIDES corresponding to amino acids surrounding the PHOSPHORYLATION site. (jefferson.edu)
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (joplink.net)
Peptide15
- The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6). (phosphosolutions.com)
- Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. (phosphosolutions.com)
- Synthetic peptide corresponding to Human Histone H3 (phospho S10). (abcam.com)
- The antibody was used in the absence (top left) or presence (top right) of blocking peptide (VX2785). (ecmbio.com)
- Phospho-VASP (Thr-278) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding threonine 278 in human VASP. (ecmbio.com)
- This antibody was affinity purified using phospho-VASP (Thr-278) peptide. (ecmbio.com)
- Phospho-Crk (Tyr-221) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-221 in human Crk II. (phosphosolutions.com)
- Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y951). (abcam.com)
- AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX. (prosci-inc.com)
- 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. (avivasysbio.com)
- This antibody is purified through a protein A column, followed by peptide affinity purification. (avivasysbio.com)
- Phosphospecific peptide around Tyr467/199 of Human PI3 Kinase p85/p55. (arigobio.com)
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.cn)
- These peptide usually contains the epitope recognized by the antibody. (affbiotech.com)
- Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
Specificity2
- We have extensive experience developing high-specificity phospho-specific antibodies for our customers. (neobiolab.com)
- Specificity This antibody detects endogenous human, mouse, and rat GATA1 only when phosphorylated at serine 310. (qedbio.com)
Secondary antibodies1
- Immunocytochemical labeling of VASP phosphorylation in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal VASP (Thr-278) antibody, then detected using appropriate secondary antibodies conjugated to Cy3. (ecmbio.com)
Rabbit9
- We identify rabbit monoclonal phospho-specific antibodies (MJFF-pRAB10) that are exquisitely selective for LRRK2-phosphorylated Rab10, detecting endogenous phosphorylated Rab10 in all analysed cell lines and tissues, including human brain cingulate cortex. (dundee.ac.uk)
- antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4). (ecmbio.com)
- Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. (ecmbio.com)
- The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. (abcam.com)
- A UW-Madison researcher has developed a rabbit polyclonal antibody for methylated PKM2. (warf.org)
- Anti-rabbit IgG, HRP-linked Antibody ( #7074 ). (cellsignal.com)
- The antibody is a rabbit polyclonal antibody raised against NSE. (uscnk.com)
- It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. (bd-ibr.org)
- Description: A Rabbit Polyclonal antibody against Cdc25C (phospho Ser216) from Human. (bd-ibr.org)
Detects endogenous levels3
- This antibody detects endogenous levels of PI3 Kinase p85/p55 protein only when phosphorylated at Tyr467/199. (arigobio.com)
- Phospho-NF-kB p65 (Thr254) Antibody detects endogenous levels of NF-kB p65 only when phosphorylated at Threonine 254. (affbiotech.cn)
- Phospho-NF kappaB p100/p52 (Ser872) Antibody detects endogenous levels of NF kappaB p100/p52 only when phosphorylated at Serine 872. (affbiotech.com)
Endogenous1
- This antibody detects endogenous level of AFX only when phosphorylated at serine 197. (prosci-inc.com)
Proteins9
- Recent work has revealed that LRRK2 phosphorylates a subgroup of 14 Rab proteins, including Rab10, at a specific residue located at the centre of its effector-binding switch-II motif. (dundee.ac.uk)
- In the present study, we analyse the selectivity and sensitivity of polyclonal and monoclonal phospho-specific antibodies raised against nine different LRRK2-phosphorylated Rab proteins (Rab3A/3B/3C/3D, Rab5A/5B/5C, Rab8A/8B, Rab10, Rab12, Rab29[T71], Rab29[S72], Rab35 and Rab43). (dundee.ac.uk)
- The antibodies described in the present study will help with the assessment of LRRK2 activity and examination of which Rab proteins are phosphorylated in vivo. (dundee.ac.uk)
- Phospho-specific antibodies are widely used to identify the activation of signal transduction pathways resulting from the phosphorylation of a diverse set of proteins that include enzymes, receptors, and transcription factors. (neobiolab.com)
- Lipid research encompasses the large-scale profiling of cellular lipids, the quantification of specific lipids in biological samples and the documentation of the interactions between lipids and other lipids, proteins and metabolites within the body. (sanbio.nl)
- The therapeutic and biochemical properties of proteins including antibodies can be enhanced by custom chemical functionalization that enables modifications, such as small molecule drug conjugation, PE. (warf.org)
- To assay the expression level of a protein, or the phosphorylation state of a signaling protein, Western blots are often employed, with phospho-specific antibodies used to specifically detect the phosphorylated states of the proteins in question. (activemotif.com)
- Flow chart of the FACE In-Cell Western method that uses a cell-based ELISA to measure the levels of the native and phospho forms of signaling proteins and kinases that are activated by phosphorylation. (activemotif.com)
- PI3K (phospho inositide 3 kinases) inhibitors are a group of closely related kinase proteins. (lls.org)
Datasheet2
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
- The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. (ptglab.com)
Antigen1
- This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Reactivity1
- 28740-1-AP targets Phospho-EIF2S1 (Ser51) in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples. (ptglab.com)
Flow Cytometry1
- Evaluation of interferon-beta neutralizing antibodies in individual patients with multiple sclerosis based on phospho-specific flow cytometry. (uib.no)
Custom antibody services1
- Founded in 2001, we are committed to offering the highest quality antibodies, custom antibody services, and customer support in the business. (phosphosolutions.com)
Dilution4
- Dilution: 1:20 using IHC-Tek TM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. (ihcworld.com)
- Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28740-1-AP (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:2000 incubated 4℃ overnight. (ptglab.com)
- Please refer to primary antibody product webpage for recommended antibody dilution. (cellsignal.com)
- Western blot: COS7 cells treated with of without H2O2 stained with ARG66216 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / 199) antibody at 1:1000 dilution. (arigobio.com)
Clone3
- No. 425401) then stained with anti-human CD20 APC and anti-STAT5 Phospho (Tyr694) (clone A17016B.Rec) PE/Cyanine7. (biolegend.com)
- This recombinant antibody clone is derived from clone A17016B. (biolegend.com)
- Antibodies produced by a single clone of cells. (bvsalud.org)
Thermo Fisher Sci2
- The following product was used in this experiment: Phospho-EGFR (Tyr1068) Monoclonal Antibody (EP38Y) from Thermo Fisher Scientific, catalog # MA5-14485, RRID AB_10977041. (thermofisher.com)
- The following product was used in this experiment: Phospho-STK39 (Ser309) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-37792, RRID AB_2554400. (thermofisher.com)
Neuroscience1
- PhosphoSolutions is your source for neuroscience and cancer Antibodies that Work™ made and tested right here in Denver, Colorado. (phosphosolutions.com)
Protein Phosphorylation1
- Protein phosphorylation is most easily studied with phosphorylation state-specific antibodies, and has historically been studied by Western blot analysis, which is a reliable technique that detects the protein in question and also indicates the molecular weight of the target protein, as an additional control. (activemotif.com)
Signal Transduction1
- Rockland Immunochemicals, Inc. offers a complete spectrum of signal transduction antibodies. (biosciregister.com)
Isoform2
- PKC isoform expression and activities will be measured using antibodies to PKC-θ, β, λ and ζ. (umassmed.edu)
- Finally, inhibition of general and isoform A specific STAT5 significantly reduced the secretion of TNFa, IL-8 and IL-10 in ex vivo tissue slices of advanced human atherosclerotic plaques. (bvsalud.org)
Lanes1
- The blot was treated with alkaline phosphatase to dephosphorylate Crk II (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk II (C-terminal region) CM3321 (lanes 1 & 2) and Crk II (Tyr-221) phospho-specific CP4701 (lanes 3 & 4). (phosphosolutions.com)
Reagents1
- UW-Madison researchers have developed and characterized nine murine monoclonal antibody (mAb) reagents that react uniquely with the EC1 (2x), EC2 (2x), EC5 (3x), and EC6 (2x) extracellular cadherin re. (warf.org)
Recombinant1
- The antibody detects a RhoA GST fusion protein when phosphorylated with PKA, but does not detect the unphosphorylated recombinant protein. (phosphosolutions.com)
Target antibody2
- A far simpler method is to use In-Cell ELISAs, in which cells are grown in multi-well plates, fixed, and stained directly with the target antibody. (activemotif.com)
- Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. (affbiotech.com)
Phosphopeptide5
- The antibody was purified using epitope specific phosphopeptide. (abcam.com)
- The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. (abcam.com)
- Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. (prosci-inc.com)
- Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. (prosci-inc.com)
- This MEF2C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S387 of human MEF2C. (avivasysbio.com)
Chromatography1
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. (biolegend.com)
Human4
- The antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk II on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. (phosphosolutions.com)
- Immunohistochemistry-Paraffin: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of paraffin-embedded Human prostate tissue using anti-Tal1 mouse monoclonal antibody. (novusbio.com)
- Description: A polyclonal antibody for detection of Cdc25C phospho Ser216) from Human. (bd-ibr.org)
- Description: A polyclonal antibody for detection of Cdc25A phospho Thr507) from Human, Mouse, Rat. (bd-ibr.org)
Kinases1
- Site-specific phosphorylation of CXCR4 is dynamically regulated by multiple kinases and results in differential modulation of CXCR4 signaling. (jefferson.edu)
Lysates2
- Briefly, PI 3-kinase activity will be assessed by immunoprecipitating tissue lysates with a polyclonal IRS antibody coupled to protein A-sepharose beads and assessing the incorporation of 32 P into PI. (umassmed.edu)
- Akt activity will be determined by immunoprecipitating tissue lysates with a polyclonal Akt antibody, that recognizes both Akt1 and Akt2. (umassmed.edu)
Membrane3
- OAAB16210) and anti-MEF2C Non Phospho-specific Pab on nitrocellulose membrane. (avivasysbio.com)
- The membrane was stripped and re-blotted with GAPDH antibody as loading control. (ptglab.com)
- For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. (cellsignal.com)
Primary1
- Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)
Lysate1
- ELISA assays are simpler in that electrophoresis is not required, but a standard ELISA still requires the preparation of a lysate, and attachment of a capture antibody to a plate. (activemotif.com)
Immunofluorescence1
- Immunocytochemistry/ Immunofluorescence: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tal1. (novusbio.com)
Glycerol1
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. (prosci-inc.com)
Mouse1
- Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. (novusbio.com)
Inhibitor2
- We demonstrate that the MJFF-pRAB10 antibodies can be deployed to assess enhanced Rab10 phosphorylation resulting from pathogenic (R1441C/G or G2019S) LRRK2 knock-in mutations as well as the impact of LRRK2 inhibitor treatment. (dundee.ac.uk)
- Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. (caslab.com)
Descriptor1
- Antibodies, Phospho-Specific" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
Modifications1
- No changes or modifications were done to the antibody sequence or scaffold. (biolegend.com)
Assays2
- Assays for Infliximab Drug Levels and Antibodies: A Matter of Scales and Categories. (uib.no)
- However, by screening antibodies and running appropriate controls, it is possible to validate certain antibodies for in-cell ELISA assays, sometimes referred to as In-cell Westerns . (activemotif.com)