Loading...
  • bnAbs
  • These antibodies can neutralize a wide range of genetically variable HIV isolates in laboratory tests, and studies show that some bNAbs can protect monkeys against a virus similar to HIV. (iavi.org)
  • B roadly neutralizing antibodies (bNAbs) hold the potential of being a component of an HIV functional cure regimen, an HIV treatment, and even a prevention therapy to protect against the virus. (aumag.org)
  • A ) bNAbs can prevent infection by directly neutralizing cell-free virions, which prevents infection and accelerates viremia decline through antibody-mediated viral clearance. (jci.org)
  • Further, while bNAbs isolated from people with HIV infection have demonstrated promise in primate studies and have entered human studies for HIV prevention and treatment, questions remain about whether effective antibodies could be produced rapidly and at a scale suitable for widespread distribution. (nih.gov)
  • A minority of people living with HIV produce bNAbs, but only after a significant period of infection, at which point virus in their body has already evolved to resist these defenses," said Dennis R. Burton, Ph.D., a lead author on the study, director of the NIH's Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and scientific director of the IAVI Neutralizing Antibody Consortium at TSRI. (nih.gov)
  • assays
  • Cell-based assays mimic the mechanism by which the neutralizing antibodies (NAbs) exert their effect in vivo and are the regulatory authorities' preferred method to detect NAb presence. (intertek.com)
  • Cell-based neutralizing antibody assays can be difficult to develop as the selection of the most suitable assay type (indirect or direct) and format depends on thorough understanding of the drug's mechanism of action. (intertek.com)
  • Through the intelligent and experienced application of cell-based neutralizing antibody assays, Intertek is the ideal partner for your biologic drug development or pharmacovigilance requirements for immunogenicity testing . (intertek.com)
  • Henipavirus serological analyses (Luminex multiplexed binding and inhibition assays, virus neutralisation tests and western blots) and lyssavirus serological analyses (LBV: modified Fluorescent Antibody Virus Neutralisation test, LBV and Mokola virus: lentivirus pseudovirus neutralisation assay) were undertaken on 73 and 70 samples respectively. (plos.org)
  • However, the presence of antibodies against henipaviruses was detected using the Luminex binding assay and confirmed using alternative assays. (plos.org)
  • Neutralising antibodies to LBV were detected in one bat using both assays. (plos.org)
  • Vaccines
  • Researchers hope to use the knowledge of these antibodies' binding sites on HIV to develop vaccines that stimulate a long-term perhaps lifetime protective antibody response against those same vulnerable sites. (medindia.net)
  • As we develop and test new HIV vaccines, it will be possible to analyze not just antibodies in the blood, but also the specific B-cell genes that are responsible for producing antibodies against HIV," said John R. Mascola, M.D., deputy director of the VRC and co-principal investigator of the study. (medindia.net)
  • Conventionally, vaccines are screened for induction of a neutralizing antibody response in human volunteers before proceeding to late-stage clinical trials. (pnas.org)
  • NAbs
  • Thus, arenavirus envelope glycosylation impairs the protective efficacy rather than the induction of nAbs, and thereby prevents efficient antibody-mediated virus control. (nih.gov)
  • strains
  • Our results show how different factors boost the formation of antibodies that broadly combat different viral strains. (the-scientist.com)
  • A technology, called the Immune Repertoire Capture (IRC) platform was used to sequence antibodies taken from the blood and antibody-producing cells in the bone marrow of elite neutralizers, individuals with HIV who are capable of producing antibodies effective against numerous HIV strains. (aumag.org)
  • Both of these glycans appear in most HIV strains, which helps explain why PGT 128 is so broadly neutralizing," said Katie J. Doores, a research associate in the Burton lab who was one of the report's lead authors. (medindia.net)
  • These MAbs also displayed distinct patterns of relative neutralizing potency (i.e., neutralization profiles) across a panel of diverse HCV strains, which led to complementary neutralizing breadth when they were tested in combination. (nih.gov)
  • The new findings build on last year's discovery reported by VRC scientists of three HIV antibodies, two of which could stop more than 90 percent of known global HIV strains from infecting human cells in the laboratory. (medindia.net)
  • IAVI
  • What's unexpected and unique about this antibody is that it not only attaches to the sugar coating of the virus but also reaches through to grab part of the virus's envelope protein," said the report's co-senior author Dennis Burton, a professor at The Scripps Research Institute and scientific director of the International AIDS Vaccine Initiative's (IAVI) Neutralizing Antibody Center, based on the Scripps Research La Jolla campus. (medindia.net)
  • assay
  • The method described here, termed trans-sialidase inhibition assay (TIA), enables the detection of TS-neutralizing antibodies in serum samples of different mammalian species, without the use of conjugated secondary reagents. (nih.gov)
  • bone marrow
  • Sera from 23 of these patients were positive for r-HuEpo-neutralizing antibodies, and their bone marrow biopsies indicated pure red-cell aplasia, indicating the loss of erythroblasts. (ebscohost.com)
  • Sera and bone marrow biopsies from the remaining seven patients were negative for anti-r-HuEpo antibodies and red-cell aplasia, respectively. (ebscohost.com)
  • elicits
  • To make a vaccine that elicits VRC01-like antibodies, we will need to coach B cells to evolve their antibody genes along one of several pathways, which we have now identified, from infancy to a mature, HIV-fighting form," said VRC Director Gary J. Nabel, M.D., Ph.D. (medindia.net)
  • A vaccine that elicits VRC01-like antibodies would need to coax the B-cell DNA of immature antibodies to evolve along one of these pathways. (medindia.net)
  • immature
  • LA, JOLLA, CA - April 5, 2016 - Scientists at The Scripps Research Institute (TSRI) and collaborating institutions have described the first-ever immature or "teenage" antibody found in a powerful class of immune molecules effective against HIV. (scripps.edu)
  • exert
  • Previous work showed that antibodies exert selective pressure on the virus but escape variants emerge within a short period of time. (nih.gov)
  • viremia
  • Reduced posttransplant viremia was directly correlated with antibody titers against gB consistent with a humoral response against gB being important. (pnas.org)
  • characterize
  • Technological advances have made it possible to identify, characterize, optimize, and manufacture antibodies like never before. (iavi.org)
  • mice
  • Similarly, survival was modestly prolonged in severe combined immunodeficiency mice bearing intracranial human U87 glioma xenografts treated with both anti-human CCL2 mAb and anti-mouse CCL2 antibodies (2 mg/kg/dose for each, twice a week) compared to mice treated with control IgG ( P = 0.0159). (springer.com)
  • Furthermore, i.p. administration of anti-mouse CCL2 antibody in combination with temozolomide (TMZ) significantly prolonged the survival of C57BL/6 mice bearing GL261 glioma with 8 of 10 treated mice surviving longer than 70 days, while only 3 of 10 mice treated with TMZ and isotype IgG survived longer than 70 days ( P = 0.0359). (springer.com)
  • 3BNC117
  • Discovered and developed by Dr. Michel Nussenzweig , head of the Laboratory of Molecular Immunology, Zanvil A. Cohn and Ralph M. Steinman Professor of The Rockefeller University , as well as a Howard Hughes Medical Institute investigator, 3BNC117 is one of the most effective broad-spectrum HIV neutralizing antibodies. (medindia.net)
  • effectively
  • The finding adds a new dimension to the set of possible explanations for why most people who are infected with HIV do not make neutralizing antibodies that effectively fight the virus. (emaxhealth.com)
  • The scientists also spotted a hurdle they will have to overcome as they engineer their own antibodies: The teenage VRC01 has a slightly longer amino acid chain at one site than the mature version, and this chain clashes with part of the glycoprotein shield (gp120) on HIV and prevents the antibody from effectively neutralizing the virus. (scripps.edu)
  • VRC01
  • Called VRC01, VRC02 and VRC03, these antibodies were found in blood donated for NIAID studies by an HIV-infected North American known as donor 45. (medindia.net)
  • In the new paper, scientists report discovering antibodies similar to VRC01 in the blood of two HIV-infected Africans known as donor 74 and donor 0219. (medindia.net)
  • We found a way to read the books, or genes, in this library by defining unique characteristics of VRC01-like antibodies," said Peter Kwong, Ph.D., chief of the VRC's structural biology section and co-principal investigator of the study. (medindia.net)
  • Based on their discovery of the common structure and genetic origin of the VRC01-like antibodies, the scientists devised strategies for scanning the B-cell DNA libraries of donor 45 and donor 74. (medindia.net)
  • From hundreds of thousands of antibody genes, the scientists first identified thousands that code for VRC01-like antibodies and then sorted these genes into family trees showing their evolution from their earliest stage into mature forms. (medindia.net)
  • The scientists now aim to create proteins they can deliver through a vaccine to serve as signposts that direct the development of B-cell DNA to produce VRC01-like antibodies. (medindia.net)
  • Yet the new antibody also lacked one of the key structural traits of VRC01 antibodies. (scripps.edu)
  • Further studies into the antibody's genetics and structure showed that it was a precursor to mature VRC01 antibodies-in other words, it represented a middle stage in the evolution of this class of HIV killers. (scripps.edu)
  • This finding contradicts previous studies suggesting that it can take up to 10 to 15 years for VRC01 antibodies to develop useful traits. (scripps.edu)
  • As long as you have some of those VRC01 signatures, a teenage-stage antibody can become a killer for HIV," said Zhu. (scripps.edu)
  • human
  • The scientists who conducted the study hypothesize that Apobec3 in humans might play a similar role in helping shape the neutralizing antibody response to human retroviruses such as HIV. (emaxhealth.com)
  • The principal neutralizing determinant (PND) of human immunodeficiency virus (HIV)-1 resides within the V3 loop of the envelope protein. (sciencemag.org)
  • Unlike human antibodies, cattle antibodies are more likely to bear unique features and gain an edge over complicated HIV immunogens. (nih.gov)
  • In previous experiments, the TSRI team and their collaborators observed that cattle produce antibodies with long HCDR3 loops at a much higher frequency than humans, that these HCDR3 loops are ultra-long, and that bovine immune cells may produce antibodies with effective immunogen binding through a fundamentally different mechanism than takes place in human immune cells. (nih.gov)
  • scientists
  • The findings offer insights for HIV vaccine design, and support further study of modified bovine antibodies as HIV therapeutics or prevention tools in humans, scientists reported in a paper published online today in Nature . (nih.gov)
  • evolve
  • Now we know these specialized antibodies can evolve in just one or two years," added TSRI Research Associate Yajing Chen, who served as co-first author of the study with Leopold Kong (formerly of TSRI, now at NIH), TSRI Staff Scientist Linling He, and Bin Ju, Jiandong Liu and Li Ren of the China CDC. (scripps.edu)
  • proteins
  • Virus-neutralizing activity against SPPV in lamb kidney cell culture was detected for polyclonal antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. (mdpi.com)
  • To our knowledge, this is the first report demonstrating the virus-neutralizing activities of antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. (mdpi.com)
  • correlates
  • Scholl SM, Pallud C, Beuvon F et al (1994) Anti-colony-stimulating factor-1 antibody staining in primary breast adenocarcinomas correlates with marked inflammatory cell infiltrates and prognosis. (springer.com)
  • virus
  • Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus. (curehunter.com)
  • Antibody immunoprophylaxis, if effective, could augment existing HIV prevention strategies and help limit the spread of the virus until a vaccine is developed. (iavi.org)
  • The new finding about Apobec3 suggests that this gene may influence anti-HIV antibody production and may help explain why some people who are repeatedly exposed to the virus never become infected. (emaxhealth.com)
  • The development of novel model systems to study HCV entry and neutralization has allowed a detailed understanding of the molecular mechanisms of virus-host interactions during antibody-mediated neutralization. (mdpi.com)
  • therapy
  • Dr. Dong Xie , Chairman and CSO of Frontier Biotech commented: "Antibody-mediated immunotherapy and long-acting HIV medicines can potentially improve the current antiretroviral therapy. (medindia.net)
  • Moreover, in contrast to antiretroviral therapy, the longer half-life of antibodies led to control of viraemia for an average of 60 days after cessation of therapy. (nih.gov)
  • sera
  • Here we show that sera from the vaccinated seronegative patients displayed little evidence of a neutralizing antibody response against cell-free HCMV in vitro. (pnas.org)
  • response
  • This study marks the first time this technology, called deep sequencing, has been used to track the evolution of the antibody response to HIV at the genetic level. (medindia.net)