• The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • The TU169 monoclonal antibody specifically binds to the human Major Histocompatibility Complex (MHC) Class II HLA-DQ1 and DQ2 antigens and weakly to HLA-DQ3. (bdbiosciences.com)
  • Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
  • This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. (novusbio.com)
  • The following product was used in this experiment: HLA-DR Monoclonal Antibody (LN3), Super Bright™ 436, eBioscience™ from Thermo Fisher Scientific, catalog # 62-9956-42, RRID AB_2744822. (thermofisher.com)
  • BACKGROUND: HLA-B27 positivity is normal in patients undergoing rheumatic diseases. (bvsalud.org)
  • The HLA-DR3 phenotypes seem to predispose to the formation of aCL antibodies and antinuclear antibodies (ANAs), but this has not been confirmed in patients, and particular HLA alleles associated with recurrent miscarriage have not been reported. (medscape.com)
  • The association between aPL antibodies and particular human leukocyte antigen (HLA) alleles and HLA-linked epitopes has been reported in studies of patients with lupus erythematous (eg, HLA-DR7, HLA-DR4). (medscape.com)
  • In the case of systemic lupus erythematosus (SLE) and related illnesses, many of the HLA class II alleles are associated with the presence of specific autoantibodies or clinical phenotypes. (musculoskeletalkey.com)
  • Exposure of endothelial cells to anti-b2GPI antibodies and their corresponding peptides leads to the inhibition of endothelial cell activation, as shown by decreased expression of the adhesion molecules E-selectin, intercellular adhesion molecule, and vascular cell adhesion molecule and of monocyte adhesion. (medscape.com)
  • In vivo infusion of each of the anti-b2GPI antibodies into BALB/c mice followed by administration of the corresponding specific peptides prevents the peptide-treated mice from developing experimental APS. (medscape.com)
  • Recent observations raise the hypothesis that not only the drug/chemical, but also parts of the haptenated protein or peptides may constitute the important structural determinants for antigen recognition by the TCR. (frontiersin.org)
  • HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. (thermofisher.com)
  • The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response. (thermofisher.com)
  • Methods Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (bmj.com)
  • Human tumor samples were analyzed by targeted mass spectrometry for presence of homocitrullinated peptides. (bmj.com)
  • Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)
  • The allelic variations among different HLA molecules are a major factor accounting for differences in the types of antigenic peptides to which an individual responds or in the types of T cells that are used in an immune response. (musculoskeletalkey.com)
  • Due to the artificial nature of drug/chemical-T-cell epitopes, it is not clear whether thymic selection of drug/chemical-specific T cells is a common phenomenon or remains limited to few donors or simply does not exist, suggesting T-cell receptor (TCR) cross-reactivity with other antigens. (frontiersin.org)
  • Furthermore, determination of hepatitis B antibody titers could be useful in newly diagnosed CD subjects regardless of age at diagnosis. (mdpi.com)
  • The serotype is determined by the antibody recognition of α33 subset of HLA-A α-chains. (wikipedia.org)
  • Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)
  • Interestingly, the recent data in RA indicate that the major HLA-DR associations are with anti-CCP antibody positive disease, suggesting that control of autoantibody responses may be a primary mechanism underlying these associations in RA as well. (musculoskeletalkey.com)
  • HLA-DQ antigens exits as heterodimers comprised of polymorphic transmembrane HLA-DQ alpha and beta glycoproteins. (bdbiosciences.com)
  • HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). (thermofisher.com)
  • HLA class I and class II molecules are cell surface glycoproteins, anchored to the membrane by hydrophobic transmembrane segments. (musculoskeletalkey.com)
  • Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Human Spleen tissue lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
  • The diagnosis of CD also has changed as a result of the availability of CD-specific antibody tests, based mainly on tissue transgluta- minase type 2 (TG2) antibodies. (vdocuments.net)
  • Finally, differences among 148 cases with HLA-B27 positivity and negativity suffering from ankylosing spondylitis (AS) were investigated. (bvsalud.org)
  • Following this model, the human immune system is divided into two major classes: Cellular Immunity, located in the mucous membranes of the gastrointestinal and respiratory tracts and their respective lymph nodes (outer defenses), and Humoral Immunity, with production of antigen-specific antibodies by plasma cells in the bone marrow (inner defenses). (medicalveritas.org)
  • The human leukocyte antigen ( HLA ) is a gene complex that encodes the major histocompatibility complex ( MHC ) proteins . (amboss.com)
  • INTRODUCTION: Ankylosing spondylitis (AS), and carriage of HLA-B27 gene in otherwise healthy individuals, are reportedly associated with increased mortality. (bvsalud.org)
  • The MHC, one of the most gene-rich regions in the human genome, has identified more than 200 genes. (musculoskeletalkey.com)
  • The HLA molecules and their counterparts in rodents were subsequently shown to be directly responsible for immune response differences between individuals and for determining the likelihood of graft rejection. (musculoskeletalkey.com)
  • In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. (novusbio.com)
  • The polymorphisms associated with the "shared epitope" are located on the α-helical rim (DRB1 chain) of the peptide-binding cleft, where they may interact with either the bound peptide antigen or the T cell receptor. (musculoskeletalkey.com)
  • HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. (novusbio.com)
  • METHODS: This study screened totally 1503 patients who underwent HLA-B27 examination, liver/kidney function tests, and complete blood routine examination in First Affiliated Hospital of Guangxi Medical University. (bvsalud.org)
  • RESULTS: Six factors, namely red blood cell count, human major compatibility complex, mean platelet volume, albumin/globulin ratio (ALB/GLB), prealbumin, and bicarbonate radical, were chosen with the aim of constructing the diagnostic nomogram using ML methods. (bvsalud.org)
  • Women with the clinical features of APS should be tested for 3 antiphospholipid antibodies that have proven association with the diagnosis of APS: lupus anticoagulant (LAC), anticardiolipin (aCL) antibody, and anti-beta-2glycoprotein I antibody. (medscape.com)
  • For A33, the alpha "A" chain are encoded by the HLA-A*33 allele group and the β-chain are encoded by B2M locus. (wikipedia.org)
  • An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
  • HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. (novusbio.com)
  • In addition, the homocitrullinated aldolase epitope was also detected in human tumor samples. (bmj.com)
  • Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. (thermofisher.com)
  • The diagnosis of many diseases requires an HLA-B27 examination. (bvsalud.org)
  • CONCLUSION: To conclude, the proposed ML model can effectively predict HLA-B27 and help doctors in the diagnosis of various immune diseases. (bvsalud.org)
  • The aCL antibodies bind to b2GPI, or a complex formed by this b2GPI is a platelet adhesin glycoprotein and cardiolipin. (medscape.com)
  • Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
  • During thymic selection, T cells that have not yet encountered their cognate antigen are considered naive T cells. (frontiersin.org)
  • Nevertheless, there are only an estimated 10 12 T cells in the human body ( 26 ). (frontiersin.org)
  • Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. (novusbio.com)
  • These antibodies predispose to clotting in vivo, predominantly by interfering with the antithrombotic role of PLs. (medscape.com)
  • Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. (thermofisher.com)
  • HLA class I molecules consist of a 45-kD α chain encoded within the MHC that is noncovalently associated with the 12-kD β 2 -microglobulin chain (encoded on chromosome 15). (musculoskeletalkey.com)
  • HLA class II molecules consist of noncovalently associated α (32 kD) and β (28 kD) chains, both of which are encoded within the MHC. (musculoskeletalkey.com)
  • This increase was significant for HLA-B27-positive AS (SMR 1.38, 95% CI 1.11 to 1.65). (bvsalud.org)
  • Shortened life expectancy was observed among both HLA-B27-positive AS women (SMR 1.77, 95% CI 1.09 to 2.70) and men (SMR 1.31, 95% CI 1.02 to 1.59). (bvsalud.org)
  • Increased mortality is particularly significant among women with HLA-B27-positive AS. (bvsalud.org)
  • Animals immunized with aCL or with the cofactor beta-2 glycoprotein I (b2GPI) develop clinical manifestations of APS, including fetal loss, thrombocytopenia, and neurologic and behavioral dysfunction, along with elevated levels of aPL antibodies. (medscape.com)
  • The innate immune system develops in utero and, unlike the adaptive (acquired) immune system , does not require imprinting or adaptation to specific antigens nor does it provide permanent pathogen -specific immunity . (amboss.com)
  • Although the human immune system is of almost inconceivable complexity in its detailed functions, the basic principles are quite simple, which might be compared with a medieval castle with an outer mote, an outer wall with parapets, and an inner defense wall, all of which serve to protect the king (brain and nervous system) and queen (genetic system). (medicalveritas.org)
  • The first haplotype is A33-Cw14-B44-DR13-DQ6.4 A*3303 : C*1403 : B*4403 : DRB1*1302 : DQA1*0102 : DQB1*0604 : DPB1*0401 This haplotype is found in Japan and Korea, and it is the most common 5 locus HLA type in Korea, high at 4.2%, 25 times higher than in China. (wikipedia.org)
  • OBJECTIVE: To describe and analyse the initial symptoms attributable to patients with spondyloarthritis (SpA) and their association with HLA-B27 status. (bvsalud.org)
  • HLA-A33 (A33) is a human leukocyte antigen serotype within HLA-A serotype group. (wikipedia.org)
  • Life expectancy was analysed in 377 axSpA patients having available pelvic radiographs and HLA-B27 status, comparing with matched Swiss population data. (bvsalud.org)
  • The 2004 National Institutes of Health/ Agency for Healthcare Research and Quality report and a systematic literature search on antibody tests for CD in paediatric patients covering the years 2004 to 2009 was the basis for the evidence-based recommendations on CD-specific antibody testing. (vdocuments.net)
  • A detailed evidence report on antibody testing in CD forms the basis of the guidelines and will be published separately. (vdocuments.net)
  • Any of the immature or mature forms of a granular leukocyte that in its mature form has an irregularly shaped, pale-staining nucleus that is partially constricted into two lobes, and with cytoplasm that contains coarse, bluish-black granules of variable size. (immport.org)
  • Any of the immature or mature forms of a granular leukocyte with a nucleus that usually has two lobes connected by one or more slender threads of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and which can be stained by the dye eosin. (immport.org)
  • Definitions CD is an immune-mediated systemic disorder elicited by gluten and related prolamines in genetically susceptible individuals and characterised by the presence of a variable combination of gluten-dependent clinical manifestations, CD-specific antibodies, Received and accepted September 1, 2011. (vdocuments.net)
  • During this time, the perception of CD has changed from a rather uncommon enteropathy to a common multi- organ disease with a strong genetic predisposition that is associated mainly with human leukocyte antigen (HLA)-DQ2 and HLA-DQ8. (vdocuments.net)
  • Survival in relation to HLA-B27 status in the general population was studied in UK Biobank European-ancestry participants (n=407 480, n=30 419 deaths). (bvsalud.org)
  • HLA-B27 carriage in the European-ancestry general population does not influence survival, or the risk of death due to vascular disease. (bvsalud.org)
  • A lymphocyte is a leukocyte commonly found in the blood and lymph that has the characteristics of a large nucleus, a neutral staining cytoplasm, and prominent heterochromatin. (immport.org)
  • When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. (thermofisher.com)
  • In addition, validation cohort included 147 cases with HLA-B27 positivity whereas 236 with HLA-B27 negativity. (bvsalud.org)