AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Cell CycleCell Cycle ProteinsCyclin-Dependent Kinase Inhibitor p27Cyclin D1CyclinsCyclin-Dependent KinasesCyclin-Dependent Kinase 2CDC2-CDC28 KinasesCyclin-Dependent Kinase Inhibitor p21AntibodiesRetinoblastoma ProteinCyclin D3Cyclin-Dependent Kinase 4Cyclin EG1 PhaseCyclin ACell DivisionCyclin B1CDC2 Protein KinaseS-Phase Kinase-Associated ProteinsCyclin D2Cyclin BApoptosisTumor Suppressor ProteinsCell ProliferationS PhaseAntibody SpecificityCyclin DCyclin-Dependent Kinase Inhibitor p16Molecular Sequence DataPhosphorylationAntibodies, ViralMitosisProtein-Serine-Threonine KinasesNuclear ProteinsCell Line, TumorTumor Suppressor Protein p53Cells, CulturedBlotting, WesternAntibodies, MonoclonalCell LineSignal TransductionTranscription FactorsAntibodies, BacterialDNA-Binding ProteinsMicrotubule-Associated ProteinsTumor Cells, CulturedProliferating Cell Nuclear AntigenProtein BindingImmunohistochemistryRNA, MessengerCarrier ProteinsGene Expression RegulationTransfectionFlow CytometryCell Cycle CheckpointsAntibody FormationHeLa CellsProto-Oncogene ProteinsTranscription, GeneticAntibodies, NeutralizingCell NucleusIron Regulatory Protein 1Enzyme InhibitorsRNA, Small InterferingDown-RegulationDNATime FactorsAmino Acid SequenceIron Regulatory Protein 2Gene Expression Regulation, NeoplasticFluorescent Antibody TechniqueCell DifferentiationUp-RegulationAntineoplastic AgentsBase SequenceAntibody AffinityBreast NeoplasmsAntibodies, Anti-IdiotypicBinding Sites, AntibodyG2 PhaseMutationLeucine-Responsive Regulatory ProteinEpitopesCyclin-Dependent Kinase 5HIV AntibodiesAntibodies, NeoplasmAntibodies, ProtozoanMenstrual CycleAntibodies, AntinuclearCyclin-Dependent Kinase 6Recombinant Fusion ProteinsBacterial ProteinsCross ReactionsBinding SitesRecombinant ProteinsIron-Regulatory ProteinsKineticsPromoter Regions, GeneticG2 Phase Cell Cycle Checkpoints
Primary AntibodiesProgressionPhosphorylationPromoted apoptosisInhibitorInhibitorsCDK6GenesCyclinsComplexesInhibitsDifferentiationCytoplasmicInhibitionCdk2GeneRegulatesMonoclonal antibodySuppressed cell proliferationKinasesAnti-apoptoticPolyclonalRabbitSynthesisRegulateMammalianTumor cellsRegulator of cellEpithelial cellsSubunitAzideRetinoblastomaMantle cell lymLysatesArrestHead and Neck Squamous Cell CarcMyeloidCDKsDilutionMutationsTissues and cell linesTranscriptionSpecificityKnockoutPromotesPeptidesDegradationLuminalInhibitory effectStabilityCellular processesTranscriptionalRegulationCancerSquamousOverexpressionConcentrationReplicationTargets
Primary Antibodies1
  • Anti-ZKSCAN5 (SAB4501021), anti-VEGFC (ab83905), and anti-LYVE1 (ab10278) primary antibodies were used at 1:100, 1:100, and 1:50 dilutions, respectively. (bms-911543.com)
Progression37
  • Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. (wikipedia.org)
  • Cyclin D1 was originally cloned as a breakpoint rearrangement in parathyroid adenoma and was shown to be required for progression through the G1 phase of the cell cycle to induce cell migration, angiogenesis and to induce the Warburg effect. (wikipedia.org)
  • Cyclin D1 is a protein required for progression through the G1 phase of the cell cycle. (wikipedia.org)
  • Inactive pRb allows cell cycle progression through the G1/S transition and allows for DNA synthesis. (wikipedia.org)
  • It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)
  • Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. (bdbiosciences.com)
  • These results demonstrate that ChoK plays an essential role in both normal human mammary epithelial cell proliferation and breast tumor progression. (aacrjournals.org)
  • Our results provide novel insights into the function of EBNA3C on cell progression by regulating the cyclin D2 protein and raise the possibility of the development of new anticancer therapies against EBV-associated cancers. (cusabio.cn)
  • The role of cell cycle checkpoint proteins is to integrate internal and external factors to determine whether the cell is prepared for progression of the cell cycle. (ptgcn.com)
  • The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. (creative-biolabs.com)
  • Background: The putative functions of the cellular prion protein (PrP c ) are believed to be associated with cell signaling, differentiation, survival, and cancer progression. (iiarjournals.org)
  • The progression of cells through the cell cycle is regulated by a family of protein kinases known as the cyclin-dependent kinases (CDKs). (biomedcentral.com)
  • The sequential activation of the members of this family and their phosphorylation of certain substrates promotes the progression through the cell cycle. (biomedcentral.com)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
  • Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
  • Studies [ 13 - 15 ] have shown that miR-149 plays an important role in many cancers, although its regulatory role in the FOXC1-mediated progression of gastric cancer remains unclear. (hindawi.com)
  • This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
  • The genes encoding cdks involved in G1-S progression are often amplified in B-cell malignancies, including diffuse large B-cell lymphoma (DLBCL). (omecamtivmecarbilactivator.com)
  • Abnormal expression of ribosomal proteins has an important regulatory effect on the progression of cancer. (biomedcentral.com)
  • This study examines the effect of RPL5 on the progression of colon adenocarcinoma (COAD), including cell proliferation, migration and determines its underlying molecular mechanism using CRC cell lines in vitro. (biomedcentral.com)
  • We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
  • The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)
  • Comparative biochemical studies of rapamycin and wortmannin on transforming growth factor-beta 1 (TGF-beta1)-mediated G(1)-S cell cycle progression in C3H-10T1/2 embryonic fibroblasts. (janechin.net)
  • Therefore, understanding biochemical dynamics of cell cycle progression may lead to target-specific therapy with improved side effect profiles. (janechin.net)
  • An obvious requirement for the stringent control of cell cycle progression is the prevention of deregulated proliferation - loss of control may result in tumors and cancers. (janechin.net)
  • These factors couple extracellular signals to intracellular processes to determine cell cycle progression. (janechin.net)
  • Conclusion: p63, p16, MIB, Cal A, Cys A are markedly expressed and p16 is strongly suppressed in oral cavity tumors, which suggests that the latter protein may play a role in negative regulation of cell cycle progression. (bvsalud.org)
  • Immunohistochemical identification of molecular genetic events in the progression of preneoplastic lesions to spindle cell squamous-cell carcinoma enables early detection of lesions with the potential for malignant progression, thus permitting timely intervention 1,2 . (bvsalud.org)
  • The various markers that enable assessment of the progression of preneoplastic lesions to spindle cell carcinoma include the p16 protein, which halts the cell cycle and induces apoptosis by pRb-mediated phosphorylation of cyclin-dependent kinase 4 (CDK4). (bvsalud.org)
  • Thus, further exploration of genetic regulatory networks involved in BC progression and development of precise strategies are worthy and important. (biomedcentral.com)
  • In general, the circRNA-miRNA-mRNA axis may function as an extensive regulatory network in progression of some diseases. (biomedcentral.com)
  • Important regulator of cell cycle progression. (neobiotechnologies.com)
  • This editorial highlights the relevance of interfering in cancer cell progression through the pharmacological manipulation on the cell metabolism of cyclic nucleotides such as cAMP, and on the intracellular Ca2+ signaling, which may avail the reduction of toxic effects promoted by chemotherapy, radiotherapy and immunotherapy, thus decreasing the incidence of interruption in antitumoral treatment. (researchgate.net)
  • Additionally, miR-146a silencing or restoration of FLAP led to a reduction of HepG2 cell proliferation, cell cycle progression, migration, and invasion. (biomedcentral.com)
  • Indeed, this process is controlled by the same oncoproteins and tumor suppressors that normally control cell cycle progression, cell differentiation, and cell proliferation. (oncotarget.com)
Phosphorylation10
Promoted apoptosis1
  • miR-29b suppressed cellular proliferation and promoted apoptosis of pulmonary artery smooth muscle cells, possibly through the inhibition of Mcl-1 and CCND2. (cusabio.cn)
Inhibitor13
  • The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). (thermofisher.com)
  • Meanwhile, Ibrance is a cell cycle inhibitor that targets CDK4 and CDK6, both important enzymes involved in cell division. (pharmalive.com)
  • Earlier this year, Erasca announced a clinical trial collaboration and supply agreement with Eli Lilly to evaluate another asset, ERAS-601, an oral SHP2 inhibitor, with Lilly's anti-EGFR antibody cetuximab. (pharmalive.com)
  • PMID: 10102234 [PubMed - indexed for MEDLINE] 1: Exp Cell Res 2000 Feb 1;254(2):279-86 Cell cycle arrest induced by the vitamin D(3) analog EB1089 in NCI-H929 myeloma cells is associated with induction of the cyclin-dependent kinase inhibitor p27. (drlauda.at)
  • This study demonstrates that the MCL1 inhibitor, S63845, combined with cisplatin synergizes by inducing apoptosis while also decreasing proliferation in a subset of TNBC cell lines. (bvsalud.org)
  • Here, we evaluated the in vitro cytotoxic activity of the cdk2 inhibitor CVT-313 against several human DLBCL cells. (omecamtivmecarbilactivator.com)
  • Cystatin A (Cys A), a cysteine protease inhibitor, is a precursor of proteins involves in keratinocyte keratinization, and is expressed during the late phase of differentiation of these cells. (bvsalud.org)
  • BCRC-3 functions as a tumor inhibitor to suppress BC cell proliferation through miR-182-5p/p27 axis, which would be a novel target for BC therapy. (biomedcentral.com)
  • This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. (neobiotechnologies.com)
  • Here, we investigate amplification events that underlie resistance to the MEK inhibitor selumetinib (AZD6244/ARRY-142886) in COLO205 cells, a well-characterized model for reproducible emergence of drug resistance, and show that amplifications acquired are the primary cause of resistance. (babraham.ac.uk)
  • Our findings demonstrate that acquisition of MEK inhibitor resistance often occurs through gene amplification and can be suppressed by impeding cell cycle entry in drug. (babraham.ac.uk)
  • Methods This non-randomized, multicohort, phase II study evaluated the efficacy and safety of the anti-PD-1 antibody nivolumab 240 mg administered every 2 weeks in combination with the CDK4/6 inhibitor abemaciclib 150 mg twice daily and either fulvestrant (FUL) or letrozole (LET) as a first-line or second-line treatment for HR-positive HER2-negative metastatic breast cancer. (bmj.com)
  • For this purpose, NLRP3 homology model was built and the putative protein binding region of a non-covalent inhibitor MCC950 (used as a probe) was investigated using a combination of in silico techniques. (mdpi.com)
Inhibitors8
  • Selective compounds have been developed that target either the extracellular ligand-binding region of the EGFR (including a number of monoclonal antibodies [MAbs], immunotoxins, and ligand-binding cytotoxic agents) or the intracellular tyrosine kinase region (including various small-molecule inhibitors). (medscape.com)
  • Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
  • Cyclin-dependent kinase inhibitors (CDKIs) are proteins that bind to and inhibit the activity of CDKs. (biomedcentral.com)
  • Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. (elifesciences.org)
  • The CDKN2A/B locus contains genes encoding cell cycle inhibitors, including p16 Ink4a , which have not yet been implicated in the control of hepatic glucose homeostasis. (diabetesjournals.org)
  • The use of combined MCL1 inhibitors with cisplatin in TNBC effectively initiates TAp73 anti-tumor effects on cell cycle arrest and apoptosis. (bvsalud.org)
  • Cyclin-dependent kinase (cdk) inhibitors have the potential to induce growth arrest and apoptosis in cancer cells. (omecamtivmecarbilactivator.com)
  • Cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitors synergistically increased the effectiveness of antiprogrammed cell death protein-1 (anti-PD-1)/programmed death-ligand 1 (PD-L1) antibodies in preclinical studies. (bmj.com)
CDK64
Genes20
  • Cyclin D1 also binds to histone acetylases and histone deacetylases to regulate cell proliferation and cell differentiation genes in the early to mid-G1 phase. (wikipedia.org)
  • Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. (bdbiosciences.com)
  • This task can be accomplished by replacing tumor suppressor genes that have been lost or mutated, selectively inserting genes that produce cytotoxic substances, or modifying the immune system to destroy the tumor cells. (medscape.com)
  • Expression of the estrogen receptor and estrogen-induced genes was studied at the mRNA level by RT-PCR and at the protein level by immunohistochemistry and western blot analysis. (biomedcentral.com)
  • Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
  • Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
  • In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
  • Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
  • Selumetinib causes long-term G1 arrest accompanied by reduced expression of DNA replication and repair genes, but cells stochastically re-enter the cell cycle during treatment despite continued repression of pERK1/2. (babraham.ac.uk)
  • Here, we show that c-Myc induction during cell cycle entry leads to a transient decrease in Mnt-Max complexes and a transient switch in the ratio of Mnt-Max to c-Myc-Max on shared target genes. (silverchair.com)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • A) Luciferase reporter genes were determined in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with Glutaminase-IN-1 different concentrations of reporter and myc-ZKSCAN5. (bms-911543.com)
  • The genes that have been identified to date have been categorized into two broad categories, depending on their normal functions in the cell. (cancerquest.org)
  • Genes whose protein products stimulate or enhance the division and viability of cells. (cancerquest.org)
  • This first category also includes genes that contribute to tumor growth by inhibiting cell death. (cancerquest.org)
  • Genes whose protein products can directly or indirectly prevent cell division or lead to cell death. (cancerquest.org)
  • Many of these genes are responsible for providing the positive signals that lead to cell division. (cancerquest.org)
  • As stated in the introduction to this section, the defective versions of these genes, known as oncogenes, can cause a cell to divide in an unregulated manner. (cancerquest.org)
  • This is in contrast with tumor suppressor genes which must BOTH be defective to lead to abnormal cell division. (cancerquest.org)
  • Despite the differences in their normal roles, these genes all contribute to unregulated cell division if they are present in a mutant (oncogenic) form. (cancerquest.org)
Cyclins6
  • Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (thermofisher.com)
  • Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)
  • Cyclins regulate the cell cycle in association with cyclin dependent kinases (CDKs). (biomedcentral.com)
  • D-type cyclins are usually synthesized by mid-G1 phase and accumulate to a maximum as cells advance through the G1/S boundary. (biomedcentral.com)
  • Cyclins and CDKs assemble into complexes with one another as cells progress through G1 phase, cyclins being required to activate the serine-threonine kinase activity of their catalytic partners. (biomedcentral.com)
  • The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)
Complexes3
  • Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. (cusabio.cn)
  • Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. (cusabio.com)
  • Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. (neobiotechnologies.com)
Inhibits2
  • Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. (cusabio.cn)
  • In previous studies, we found that 2'-hydroxyflavonone (2HF), a citrus flavonoid, inhibits the growth of renal cell carcinoma in a VHL-dependent manner. (oncotarget.com)
Differentiation6
  • Independent of CDK, cyclin D1 binds to nuclear receptors (including estrogen receptor α, thyroid hormone receptor, PPARγ and AR ) to regulate cell proliferation, growth, and differentiation. (wikipedia.org)
  • This family, which includes epidermal growth factor receptor (EGFR), plays a pivotal role in normal cell growth, lineage determination, repair, and functional differentiation. (medscape.com)
  • Cells alter the process of transcriptional regulation so as to adjust to or drive changes in the cellular conditions between different stages of the cell cycle, stem cell differentiation or cancer development. (biomedcentral.com)
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • Another protein, calgranulin A (Cal A), is involved in the regulation of several cell processes, including the cell cycle and cell differentiation. (bvsalud.org)
  • Histone deacetylase (HDAC) plays a pivotal role in regulating the level of cellular DNA acetylation and participate in cell growth, differentiation, and apoptosis. (dovepress.com)
Cytoplasmic5
  • Factors mandating cellular consequence include cell surface-receptors, cytoplasmic proteins, and nuclear proteins. (janechin.net)
  • The mammalian cell is furnished with receptors linked to interactive series of cytoplasmic networks for controlling cellular processes. (janechin.net)
  • Perinuclear anti-neutrophil cytoplasmic antibody (P-ANCA), cytoplasmic anti-neutrophil cytoplasmic antibody (C-ANCA) and antinuclear antibody (ANA) had been also adverse. (nanoker-society.org)
  • D) Cytoplasmic and nuclear ZKSCAN5 protein levels in two types of breast cancer cell lines, ZR75-1 and MDA-MB-231. (bms-911543.com)
  • Tubulin was used as the cytoplasmic control, and lamin A/C was used as the nuclear protein-loading control. (bms-911543.com)
Inhibition7
  • Preclinical evidence supports synergistic anti-tumor effects when downstream RAS/MAPK pathway inhibition is combined with cell cycle inhibition in CRC and PDAC," he said. (pharmalive.com)
  • In MDA-MB-231 cells, ST caused a significant dose-dependent cell growth inhibition by 31- 63% (p ≤ 0.0001) in 48 h and 40-50% (p ≤ 0.0001) in 72 h. (biomedcentral.com)
  • Conclusion: Combination of fucoidan with silencing of PrP c has a synergic effect on the inhibition of HT29 colon cancer cell growth. (iiarjournals.org)
  • This study provides fundamental insight into the functional impact of CDK4 inhibition on cells in the tumor microenvironment, which is of high importance and interest to the field. (elifesciences.org)
  • Importantly, inhibition of cdk2 with CVT-313 or knockdown of endogenous cdk2 with siRNA resulted in downregulation of the anti-apoptotic factor Myeloid cell leukemia-1 (Mcl-1), suggesting that decreased levels of cellular Mcl-1 contribute to apoptosis. (omecamtivmecarbilactivator.com)
  • ERKi treatment of cells drives the poly-ubiquitylation and proteasome-dependent turnover of ERK2 and pharmacological or genetic inhibition of Cullin-RING E3 ligases prevents this. (babraham.ac.uk)
  • Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
Cdk23
  • Cyclin D1-CDK4 also enables the activation of cyclin E-CDK2 complex by sequestering Cip/Kip family CDK inhibitory proteins p21 and p27, allowing entry into the S phase. (wikipedia.org)
  • Cyclin A is involved in both S-phase and G2/M transitions of the cell cycle through its association with cdk2 and cdk1, respectively. (bdbiosciences.com)
  • Depletion of endogenous cdk2 by short interfering (si)RNA also resulted in apoptosis in human LY3, LY8 and LY18 DLBCL cells. (omecamtivmecarbilactivator.com)
Gene18
  • Cyclin D1 is a protein that in humans is encoded by the CCND1 gene. (wikipedia.org)
  • The CCND1 gene encodes the cyclin D1 protein. (wikipedia.org)
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. (wikipedia.org)
  • This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. (wikipedia.org)
  • Amplification of the CCND1 gene is present in: non-small cell lung cancers (30-46%) head and neck squamous cell carcinomas (30-50%) pancreatic carcinomas (25%) bladder cancer (15%) pituitary adenomas (49-54%) breast carcinoma (13%) Cyclin D1 overexpression is strongly correlated to ER+ breast cancer and deregulation of cyclin D1 is associated with hormone therapy resistance in breast cancer. (wikipedia.org)
  • This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. (thermofisher.com)
  • Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
  • The goal of gene therapy is to introduce new genetic material into cancer cells that selectively kills them without causing toxicity to the surrounding cells. (medscape.com)
  • Using a composite gene-set analysis method, we combine the information of transcription factor binding sites, Gene Ontology or pathway gene sets and gene expression fold-change profiles for a variety of cell conditions. (biomedcentral.com)
  • Concomitantly, downregulation of estrogen receptor (ER alpha), the estrogen-responsive gene - vascular endothelial growth factor (VEGF), and G1 cell cycle proteins, cyclin D1 and CDK4, was also observed. (biomedcentral.com)
  • By applying the random forests algorithm, a supervised machine learning approach, we show how a multi-gene signature that classifies individual cells into their correct cell cycle phase and cell size can be generated. (frontiersin.org)
  • Modern approaches to treating cancer take advantage of critical biochemical differences between cancer cells and normal cells - from radiation therapy to chemotherapy to experimental gene therapy. (janechin.net)
  • Expression of p63 is almost exclusively restricted to epithelial cells, mutations in this gene are infrequent, and its expression is increased in a variety of solid tumors, particularly those of the head and neck area 12,13 . (bvsalud.org)
  • The oncogenic activity of Myc family proteins is initiated when their transcriptional regulation is disrupted by events such as gene amplification and gene translocation, which typically lead to elevated cellular Myc levels. (silverchair.com)
  • B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)
  • Note that by convention gene names are italicized and the proteins they make are not. (cancerquest.org)
  • As an example TP 53 refers to the gene and p53 refers to the protein. (cancerquest.org)
  • We have two copies of each gene and for oncogenes, a single defective copy is enough to cause a cell to divide. (cancerquest.org)
Regulates3
  • Here, we review the major advances underpinning our current understanding of how the elasticity of single proteins regulates mechanosensing and mechanotransduction. (ibecbarcelona.eu)
  • In addition to its effects on dMyc, Puf regulates both Ago and its cell cycle substrate Cyclin E. Therefore, Puf influences cell growth by controlling the stability of key regulatory proteins. (silverchair.com)
  • The conditioned medium from these cells re-expressing ZKSCAN5 could rescue these effects ( Figures?2A, B ). A similar tendency was also detected in HLEC migration analysis ( Figures?2C, D ). Open in a separate window Figure?2 VEGFC secreted by cancer cells, under the influence of ZKSCAN5, regulates HLEC proliferation, migration, and tube formation. (bms-911543.com)
Monoclonal antibody2
  • Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • SDS-PAGE Analysis Purified p27 Mouse Monoclonal Antibody (KIP1/1357). (neobiotechnologies.com)
Suppressed cell proliferation1
Kinases2
Anti-apoptotic2
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • One mechanism employed by TNBC to avoid cell death is the increased expression of the anti-apoptotic protein, myeloid cell leukemia 1 (MCL1). (bvsalud.org)
Polyclonal1
  • The following product was used in this experiment: CDK4 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 11026-1-AP. (thermofisher.com)
Rabbit2
  • Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (KD/KO validated CDC25A antibody) at a dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptgcn.com)
  • Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.cn)
Synthesis5
  • Ribosomal protein (RP) is a widely existing RNA-binding protein that was previously thought to function only as a combination of ribosomal RNA (rRNA) involved in protein synthesis. (biomedcentral.com)
  • The ribosomal protein RPL5 is a component of the ribosome, a large ribonucleoprotein complex responsible for protein synthesis in cells. (biomedcentral.com)
  • Stages of the cell cycle are G1 (Gap 1), S (Synthesis), G2, M (Mitosis), and G0 (quiescence), with mitosis as the shortest and most visually dramatic process of the cycle after which two daughter cells result, Figure 1-1. (janechin.net)
  • The alterations of ribosome biogenesis and protein synthesis play a direct role in the development of tumors. (oncotarget.com)
  • Ribosome biogenesis, the process of ribosome production, is frequently up-regulated in cancer in order to respond to the increased demand of protein synthesis in highly proliferating cells. (oncotarget.com)
Regulate8
  • The protein dimerizes with CDK4/6 to regulate the G1/S phase transition and entry into the S-phase. (wikipedia.org)
  • data indicate that linc00598 plays an important role in cell cycle regulation and proliferation through its ability to regulate the transcription of CCND2. (cusabio.cn)
  • One main reason for the difficulty is the dynamic nature of the networks: Transcription factors (TFs) not only regulate different targets depending on the cell conditions, but their effects on these targets can also change. (biomedcentral.com)
  • However, the molecular mechanisms that regulate force-induced functionalities across a wide range of scales, encompassing the cell, tissue or organ levels, are not well understood in comparison. (ibecbarcelona.eu)
  • With the advent, development and refining of single-molecule nanomechanical techniques that enable the conformational dynamics of individual proteins under the effect of a calibrated force to be probed, we have begun to acquire a comprehensive knowledge of the diverse physicochemical principles that regulate the elasticity of single proteins. (ibecbarcelona.eu)
  • The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. (cusabio.com)
  • Some proto-oncogenes work to regulate cell death. (cancerquest.org)
  • The mutant proteins often retain some of their capabilities but are no longer sensitive to the controls that regulate the normal form of the protein. (cancerquest.org)
Mammalian2
  • The mammalian cell cycle is stringently regulated and orderly process by which a cell reproduces. (janechin.net)
  • The mammalian cell cycle typically completes in 24 hours, where dramatic changes occur in cellular metabolism and cytoskeletal physiology. (janechin.net)
Tumor cells4
  • This action allows Ibrance to arrest the frenzied growth and multiplication of tumor cells, preventing the malignancy from progressing. (pharmalive.com)
  • however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. (nature.com)
  • Uncontrolled cell proliferation is one of the greatest features of all tumor cells, and there is abundant evidence that blocking cell proliferation or cell cycle processes has the potential to alleviate or cure tumors [ 4 , 5 ]. (biomedcentral.com)
  • Abnormal expression of 5-Lipoxygenase Activating Protein (FLAP) has been detected in many tumor cells. (biomedcentral.com)
Regulator of cell2
  • cyclin D2 acts as regulator of cell cycle proteins affecting SAMHD1-mediated HIV-1 restriction in non-proliferating macrophages. (cusabio.cn)
  • The c-Myc oncoprotein is strongly induced during the G0 to S-phase transition and is an important regulator of cell cycle entry. (silverchair.com)
Epithelial cells3
  • Here, we have investigated the role of ChoK in the development of breast cancer and found that ChoK is both necessary and sufficient for growth factor-induced proliferation in primary human mammary epithelial cells and an absolute requirement for the specific mitogenic response to heregulin in breast tumor-derived cells. (aacrjournals.org)
  • Histological evaluation revealed degeneration of luminal epithelial cells and endometrial glands in response to arsenic treatment, along with reduction in thickness of the longitudinal muscle layer. (biomedcentral.com)
  • We studied the consequences of stable inducible JHDM1B silencing in cell lines derived from transformed and untransformed mammary epithelial cells. (oncotarget.com)
Subunit2
  • Although DBF4-dependent kinase (DDK) complex composed of CDC7 kinase and its regulatory subunit DBF4 has been shown to be overexpressed in primary tumors and promotes tumor development, while its role and prognostic value in HCC remain largely unknown. (biomedcentral.com)
  • The DBF4-dependent kinase (DDK) composed of CDC7 kinase and its regulatory subunit DBF4, which is required for CDC7 kinase activity, is a critical regulator of DNA replication by catalyzing MCM helicase (MCM2-7) (Fig. 1 A) [ 6 , 7 ]. (biomedcentral.com)
Azide2
  • Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. (neobiotechnologies.com)
  • 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. (neobiotechnologies.com)
Retinoblastoma3
Mantle cell lym1
Lysates1
  • In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. (neobiotechnologies.com)
Arrest5
  • While exploring the molecular changes associated with strong ST efficacy in breast cancer cells, we observed that ST induced cell cycle arrest as well as cell death. (biomedcentral.com)
  • ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)
  • EB1089 (1 x 10(-8) M) efficiently induced G(1) arrest of the cell cycle. (drlauda.at)
  • Combined treatment with selumetinib and a dose of palbociclib sufficient to reinforce G1 arrest in selumetinib-sensitive cells, but not to impair proliferation of resistant cells, delays the emergence of resistant colonies, meaning that escape from G1 arrest is critical in the formation of resistant clones. (babraham.ac.uk)
  • The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. (abcam.cn)
Head and Neck Squamous Cell Carc3
  • [ 1 ] Early stage head and neck squamous cell carcinoma (HNSCC) is treated relatively well with single-modality therapy (either surgery or radiation alone). (medscape.com)
  • Go to Imaging of Nasopharyngeal and Laryngeal Squamous Cell Carcinoma and Head and Neck Squamous Cell Carcinoma for complete information on these topics. (medscape.com)
  • The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)
Myeloid2
  • Cyclin D1 is expressed in all adult human tissues with the exception of cells derived from bone marrow stem cell lines (both lymphoid and myeloid). (wikipedia.org)
  • Here, we demonstrate a novel binding interaction between the first RNA recognition motif 1 (RRM1) of PTBP1 and the prosurvival protein myeloid cell leukemia-1 (MCL1). (bvsalud.org)
CDKs1
Dilution2
  • Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55004-1-AP (Cyclin B1 Antibody) at a dilution of 1:200 (under 10x lens). (ptgcn.com)
  • Western blot of Knockout validated Cyclin D1 antibody in HepG2, SW 1990, and NIH/3T3 cell lines with 60186-1-Ig at a dilution of 1:10000 incubated at room temperature for 1.5 hours. (ptgcn.com)
Mutations2
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Melanoma in particular exhibits a high incidence of activating BRAF and NRAS mutations and such cells are addicted to the activity of these mutant oncoproteins. (babraham.ac.uk)
Tissues and cell lines3
  • In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
  • The expression level of RPL5 in colon cancer tissues and cell lines was significantly higher than that in adjacent tissues and NCM460 cells, respectively, and its expression level was higher in HCT116 cells and RKO cells. (biomedcentral.com)
  • BCRC-3 was lowly expressed in bladder cancer tissues and cell lines. (biomedcentral.com)
Transcription5
Specificity1
  • While several mechanisms of RBP specificity have been proposed (e.g., cell-specific expression of RBPs and secondary structure of target RNA), recently, protein-protein interactions with individual domains of RBPs have been suggested to be important determinants of downstream function. (bvsalud.org)
Knockout1
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
Promotes4
  • The CD274/JNK/Cyclin D2 pathway promotes the cell cycle entry of LIC. (cusabio.cn)
  • Here, we demonstrate that RPL5 promotes COAD cell proliferation and migration via the MAPK/ERK signaling pathway. (biomedcentral.com)
  • ZKSCAN5-Regulated VEGFC Promotes the Proliferation, Migration, and Tube Formation of HLECs Cancer cell-secreted VEGFC markedly enhanced the proliferation and migration of lymphocyte endothelial cells. (bms-911543.com)
  • This study showed that miR-146a has a stimulatory role in HepG2 cells and promotes HepG2 cell migration and invasion by targeting FLAP mRNA. (biomedcentral.com)
Peptides1
Degradation2
  • In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)
  • Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. (cusabio.com)
Luminal1
  • Together, the results indicate that arsenic disrupted the circulating levels of gonadotropins and estradiol, led to degeneration of luminal epithelial, stromal and myometrial cells of the rat uterus and downregulated the downstream components of the estrogen signaling pathway. (biomedcentral.com)
Inhibitory effect3
  • Additionally, 2HF inhibited efflux of doxorubicin and increased its accumulation in the cells, but did not add to the transport inhibitory effect of anti-Rlip antibodies alone. (oncotarget.com)
  • Moreover, TBHQ could partially reverse the inhibitory effect of siRPL5 on the proliferation and migration of colon cancer cells. (biomedcentral.com)
  • Finally, the inhibitory effect of the combined chidamide and bortezomib treatment on MGC-803 cells was verified in vivo through tumor formation experiments in nude mice. (dovepress.com)
Stability3
  • threonine residue (threonine 286) that controls nuclear export and protein stability. (wikipedia.org)
  • Polypyrimidine tract binding protein 1 (PTBP1) is one of the most well-described RNA binding proteins, known initially for its role as a splicing repressor before later studies revealed its numerous roles in RNA maturation, stability, and translation. (bvsalud.org)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
Cellular processes2
  • Mechanical force modulates the conformation and function of individual proteins, and this underpins many mechanically driven cellular processes. (ibecbarcelona.eu)
  • Myc proteins control multiple cellular processes, including cell growth, proliferation, metabolism and apoptosis, and deregulation of Myc plays an important role in oncogenesis ( Dang, 2012 ). (silverchair.com)
Transcriptional4
  • Genome-wide expression profiles reflect the transcriptional networks specific to the given cell context. (biomedcentral.com)
  • It is able to provide useful clues for novel cell-specific transcriptional regulations. (biomedcentral.com)
  • Such changes in the transcriptional networks between different cell conditions are represented in the transcriptome data that are obtained using microarrays or high-throughput sequencing. (biomedcentral.com)
  • However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)
Regulation6
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
  • Polypyrimidine tract binding protein 1 (PTBP1) is an RBP that is involved in all steps of RNA maturation and serves as a key regulator of alternative splicing, and therefore, understanding its regulation is of critical biologic importance. (bvsalud.org)
  • Encouraging progress in understanding cell cycle regulation occurred over the past five years. (janechin.net)
  • Such regulation ensures faithful reproduction of DNA for subsequent distribution to daughter cells. (janechin.net)
  • Their de-regulation in tumors could be responsible for both a surge in ribosome biogenesis, and the loss of cell cycle control [ 1 ]. (oncotarget.com)
Cancer47
  • IHC-P analysis of human breast cancer tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
  • Our results indicate that miR-4317 can reduce Non-small cell lung cancer (NSCLC) cell growth and metastasis by targeting FGF9 and CCND2. (cusabio.cn)
  • Recent studies have examined the consequences of epigenetic marks and cell cycle control, which has led to more research regarding cell division cancer, emphasizing the fact that the cell division process requires accurate checkpoints to avoid genetic damage. (ptgcn.com)
  • With the increased understanding of molecular mechanisms and basic pathways in the pathogenesis of squamous cell cancer of the head and neck , these pathways may be modified, and rational approaches in cancer therapy at the molecular level may be created. (medscape.com)
  • The goal of specific molecular targets in cancer therapy is to create a "magic bullet" that selectively kills cancer cells. (medscape.com)
  • As our understanding of the molecular biology of HNSCC continues to develop, we can target the specific components of cancer cells that are not found in normal cells. (medscape.com)
  • Ideal targets should be both specific to cancer cells and commonly found in cancer cells. (medscape.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Our results show that 2HF induced apoptosis in both histological types of lung cancer and inhibited proliferation and growth through suppression of CDK4, CCNB1, PIK3CA, AKT and RPS6KB1 (P70S6K) signaling. (oncotarget.com)
  • Designed as an oral medication, ERAS-007 exerts its anti-cancer effect by targeting and inhibiting the ERK1 and ERK2 signaling proteins, which disables the RAS/MAPK pathway, a central cascade in cancer. (pharmalive.com)
  • Here, we evaluated the efficacy of ST on three different human cancer cell lines including skin epidermoid carcinoma A431 cells, lung epithelial carcinoma A549 cells and breast adenocarcinoma MDA-MB-231. (biomedcentral.com)
  • Cell growth assay, cell cycle analysis, FACS, JC-1 staining, annexin V staining and immunoblotting were used to study the efficacy of ST on cancer cells. (biomedcentral.com)
  • This study provides valuable insight into the chemopreventive efficacy and associated molecular alterations of ST in breast cancer cells whereas it had only moderate efficacy on lung cancer cells and did not show any considerable effect on skin cancer cells. (biomedcentral.com)
  • Materials and Methods: PrP c expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. (iiarjournals.org)
  • Results: Fucoidan treatment significantly inhibited growth and reduced cyclin and cyclin-dependent kinase (CDK) expression in HT29 colon cancer cells. (iiarjournals.org)
  • Tumor initiation, development, and relapse may be closely associated with cancer stem cells (CSCs). (nature.com)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • Furthermore, cancer cells undergoing EMT phenotypic changes also acquire drug resistance [ 7 ]. (techscience.com)
  • A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
  • The compelling conclusion that proliferative exhausted T cells are associated with response in HPV+ head and neck cancer is supported by the cohort of 14 patients with paired tumor and adjacent normal tissue and rigorous bioinformatic analysis of nearly 50,000 single CD3+ T cell transcriptomes. (elifesciences.org)
  • Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
  • Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
  • qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
  • In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
  • The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
  • More and more studies have found that abnormal expression or mutation of ribosomal proteins has an important regulatory effect on the occurrence and development of cancer [ 5 ]. (biomedcentral.com)
  • Detailly, compared with adjacent tissues, both mRNA and protein of DBF4 and CDC7 were significantly higher in HCC, and their expression was positively correlated with AJCC_T stage, clinical stage and G stage (grade) of liver cancer patients, and higher DBF4 or CDC7 expression predicted a worse prognosis in HCC patients with shorter overall survival (OS), recurrence-free survival (RFS), disease-specific survival (DSS) and progress-free survival (PFS). (biomedcentral.com)
  • Distinguishing and removing cancer cells from normal cells continue to be key in the experimental design for therapy and prevention. (janechin.net)
  • The Warburg hypothesis was based on the metabolic differences between cancer cells and normal cells, and proposed that increased glycolysis by transformed cells conferred a bio-energetic advantage for survival over normal counterparts under anoxic conditions (Anghileri, 1983). (janechin.net)
  • When cells proliferate in the absence of appropriate driving signals, cancer is the undesirable consequence. (janechin.net)
  • The effects of BCRC-3 on cancer cells were explored by transfecting with plasmids in vitro and in vivo. (biomedcentral.com)
  • We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
  • Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
  • Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
  • Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
  • Statistical Analyses Statistical significance was assessed by using the two-tailed Students in breast cancer cells. (bms-911543.com)
  • Because ZKSCAN5 improved the secretion of VEGFC by breast cancer cells, the effects of the conditioned medium on HLEC proliferation and migration were investigated in ZKSCAN5 knockdown stable cell lines. (bms-911543.com)
  • The aim of this study was to investigate the antitumor effect of chidamide in combination with bortezomib on gastric cancer cell lines. (dovepress.com)
  • First, the sensitivity and IC 50 values of chidamide and bortezomib in several gastric cancer cell lines (MGC-803, BGC-823, SGC-7901, and MKN45) were measured using the CCK-8 assay. (dovepress.com)
  • Then, the relatively insensitive gastric cancer cell lines (MGC-803 and BGC-823) were treated with low concentrations of chidamide alone, bortezomib alone, or chidamide and bortezomib combination to detect the effects on cell proliferation, apoptosis, migration, and invasion. (dovepress.com)
  • In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • Herein, we aimed to demonstrate the expression of miR-146a and FLAP in human HCC tissues and liver cancer cell lines. (biomedcentral.com)
  • Knockdown of miR-146a also resulted in increased FLAP expression of cancer cells. (biomedcentral.com)
Squamous1
  • Methods: fifteen histological specimens of spindle cell squamous cell carcinoma of the lower lip were obtained from the Department of Oral Pathology, Bahia Federal University. (bvsalud.org)
Overexpression1
Concentration1
  • ATCC CCL-243) were probed with the mouse anti-human cyclin A antibody at concentration of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3). (bdbiosciences.com)
Replication3
Targets2
  • To validate the approach, nine predicted targets of E2F1 in HeLa cells were tested using chromatin immunoprecipitation assay. (biomedcentral.com)
  • New regulatory targets were discovered and novel functions of known proteins were uncovered. (janechin.net)