• The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. (gbiosciences.com)
  • Consequently, only the imidazole will be exposed to lymphocytes, which commonly results in generation of antibodies that recognize protein-bound histamine with only limited affinity and sensitivity for free histamine. (justia.com)
  • Our Custom Peptide Antibody Production package includes everything needed to obtain custom antibodies against a synthesized peptide. (pacificimmunology.com)
  • Affinity purification of the serum against the peptide will eliminate non-specific cross reactivity in the serum and maximize specificity of the anti-peptide antibodies in your assays. (pacificimmunology.com)
  • Pacific Immunology® recommends coupling the peptide to the carrier protein via a cysteine that has been added to the N or C-terminal of the peptide. (pacificimmunology.com)
  • Pacific Immunology® typically synthesizes 40-50 mg of immunograde peptide, with 3-5 mg being required for conjugation to the carrier protein. (pacificimmunology.com)
  • Pacific Immunology® offers one of the highest titer guarantees in the industry, promising at least a 1:50,000 antibody titer against the peptide sequence. (pacificimmunology.com)
  • These opportunities are surveyed here against a background of the immunobiology of allergic sensitization and current state-of-the-art approaches to measurement of peptide/protein reactivity. (cdc.gov)
  • Current, antibody-based diagnostical methods utilize antibodies against histamine raised by conjugating histamine to a large immunogenic protein carrier, such as bovine serum albumin (BSA) or ovalbumin (OVA) via its primary amine group. (justia.com)
  • Chemokines are assigned to three different families based on the number and position of conserved cysteine residues (Van Coillie et al. (justia.com)
  • The α and β chemokines each contain four conserved cysteine residues. (justia.com)
  • By contrast, lymphotactin is the sole member of the third family of chemokines, and contains only the second and fourth conserved cysteine residues. (justia.com)
  • The mechanism by which vaccine adjuvants enhance immune responses has historically been considered to be the creation of an antigen depot. (mdpi.com)
  • In addition, molecular docking was performed to show that the structure of the fusion protein vaccine had strong affinity with B7 (B7-1, B7-2). (bvsalud.org)
  • Transmembrane envelope protein of the HUMAN IMMUNODEFICIENCY VIRUS which is encoded by the HIV env gene. (lookformedical.com)
  • Gp120 binds to cells expressing CD4 cell-surface antigens, most notably T4-lymphocytes and monocytes/macrophages. (lookformedical.com)
  • The N-terminal part of gp41 is thought to be involved in CELL FUSION with the CD4 ANTIGENS of T4 LYMPHOCYTES, leading to syncytial formation. (lookformedical.com)
  • The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. (fishersci.com)
  • The identification of Pathogen-Associated Molecular Patterns (PAMPs) has greatly advanced our understanding of how adjuvants work beyond the simple concept of extended antigen release and has accelerated the development of novel adjuvants. (mdpi.com)
  • However, inclusion of protein antigens fromStreptococcus pneumoniaecombined with potent adjuvants and a suitable delivery system are expected to both extend protection to serotype strains not represented in the formulation and stimulate a broader immune response, thus more effective in young children, elderly, and immunocompromised populations. (univ-nantes.fr)
  • Along this line, nanoparticle (NP) delivery systems can enhance the immunogenicity of antigens by protecting them from degradation and increasing their uptake by antigen-presenting cells, as well as offering co-delivery with adjuvants. (univ-nantes.fr)
  • Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
  • The mammalian genome comprises nuclear DNA (nDNA) derived from both parents and mitochondrial DNA (mtDNA) that is maternally inherited and encodes essential proteins required for oxidative phosphorylation. (regenerativemedicine.net)
  • MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
  • The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. (lookformedical.com)
  • Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. (fishersci.com)
  • Protein A affinity chromatography is widely used for the large-scale purification of antibodies because of its high yield, selectivity, and compatibility with NaOH sanitation. (bvsalud.org)
  • A general platform to produce robust affinity capture ligands for proteins beyond antibodies would improve bioprocessing efficiency. (bvsalud.org)
  • We previously developed nanoCLAMPs (nano Clostridial Antibody Mimetic Proteins), a class of antibody mimetic proteins useful as lab-scale affinity capture reagents. (bvsalud.org)
  • The improved nanoCLAMP scaffold will enable the development of robust, high-performance affinity chromatography resins against a wide range of protein targets. (bvsalud.org)
  • Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)
  • From here, the antigen is slowly released and provided to immune cells over an extended period of time. (mdpi.com)
  • IL-2 and IFN-γ), immune checkpoint blockade (ICB) therapy (e.g., anti-PD-1/PD-L1 antibodies), and adoptive T-cell transfer (e.g., chimeric antigen receptor (CAR) T-cell therapy) [ 3 - 5 ]. (thno.org)
  • Characterized by the expression of the critical transcription factor forkhead box protein P3, regulatory T (Treg) cells are an essential part of the immune system, with a dual effect on the pathogenesis of autoimmune diseases and cancer. (biomedcentral.com)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • The invention also provides cells producing the antibodies, and methods of making and using the same. (justia.com)
  • SARS-CoV-2 vaccines, composed of mRNA-containing PEGylated lipid nanoparticles, have been widely administered as intramuscular (i.m.) injections, so it is important to determine if PEGylated formulations can induce anti-PEG antibodies. (matci.jp)
  • For the best results, we recommend our Monospecific Antibody Production Package , which includes these services with a package pricing discount. (pacificimmunology.com)
  • La glyco-enzymologie au travers de laquelle l'équipe cherche à identifier, à caractériser et à modifier les propriétés d'enzymes impliquées dans le métabolisme des sucres (glycosides hydrolases, sulfatases) pour les utiliser en tant qu'outils synthétiques pour la production de molécules à haute valeur ajoutée. (univ-nantes.fr)
  • MCP-1-specific antibodies have previously been described in the literature (WO 01/89582, WO 01/89565, Luo et al. (justia.com)
  • however they fail to elicit a strong antibody response. (gbiosciences.com)
  • The use of cationized BSA is known to elicit greater immunogenic response compared to normal BSA (1-3). (gbiosciences.com)
  • Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns. (fishersci.com)
  • We have demonstrated that, in certain conditions, an intravenous (i.v.) injection of PEGylated liposomes (PEG-Lip) induced anti-PEG IgM antibodies, which led to rapid clearance of second doses in mice. (matci.jp)
  • Bound target proteins could be eluted at neutral or acidic pH. (bvsalud.org)
  • DNA sequences that form the coding region for the viral envelope (env) proteins in retroviruses. (lookformedical.com)
  • This work describes a protein engineering campaign to develop a more robust nanoCLAMP scaffold compatible with harsh bioprocessing conditions. (bvsalud.org)
  • The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. (lookformedical.com)
  • This "depot" was formed by associating the antigen with substances able to persist at the injection site, such as aluminum salts or emulsions. (mdpi.com)
  • To this end, we constructed an IL-2/IL2Ra fusion protein (IL-2FP) with an MMP2/9-specific cleavage site, designed to exploit the dysregulated protease activity in the TME to selectively activate IL-2 in the tumor. (bvsalud.org)
  • Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)
  • The DEC-205 monomer forms a compact structure comprising two intercalated rings of C-type lectin-like domains, where the N-terminal cysteine-rich and fibronectin domains reside at the central intersection. (uci.edu)
  • This is referred to as the primary structure of proteins. (lookformedical.com)