Monoclonal antibodiesClaudin-1 and occludinVEGFProteins occludinAssessed by Western blot analysisPolyclonal AntibodyProteinOCLNThermo Fisher SciEpithelial tight junctionClaudinsAntigenImmunohistochemistryEndothelial cellRabbit polyclonalIsotypeCD81ReactivityBarrier functionPhysiologicalTightHybridomasCellularGoatBiologicalDecreaseSpecificThesisImmunofluorescenceCellsHumanRoleInflammationWesternTissueCellStudyExpressionMouseCompounds
Monoclonal antibodies4
- Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
- Very recently, human monoclonal antibodies were approved for use on patients with COVID-19. (frontiersin.org)
- The human monoclonal antibodies used in this study are almost identical with these approved antibodies. (frontiersin.org)
- To better characterize the cellular functions of testisin, hybridomas producing anti-testisin monoclonal antibodies were acquired, antibodies purified, and then characterized. (umaryland.edu)
Claudin-1 and occludin1
- Recently, claudin-1 and occludin have emerged as additional receptors required for entry. (monash.edu)
VEGF5
- Detect VEGF using this Anti-VEGF Antibody, clone VG1 validated for use in WB. (sigmaaldrich.com)
- Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate. (sigmaaldrich.com)
- The expression and phosphorylation of Hif-1α, VEGF, VEGFR-2, ERK and occludin were measured by Western blot analysis. (researchsquare.com)
- In addition, the inhibitors of Hif-1α, VEGF, VEGFR-2, and ERK could reduce the effect of TNF-α on occludin expression. (researchsquare.com)
- Conclusion: TNF-α could decrease the expression of occludin via Hif-1α/VEGF/VEGFR-2/ERK signaling pathway, which was attenuated by propofol. (researchsquare.com)
Proteins occludin2
- Moreover, testisin siRNA knockdown increased the paracellular permeability to FITC-albumin across endothelial cell monolayers, which was associated with decreased expression of the adherens junction protein VE-cadherin and increased levels of phospho-(Tyr658)-VE-cadherin, without affecting the levels of the tight junction proteins occludin, claudin-5, or ZO-1. (umaryland.edu)
- We measured the effects of cytokine exposure of CF and non-CF well-differentiated main human being airway epithelial cells on TJ properties including transepithelial resistance paracellular permeability to hydrophilic solutes and the TJ proteins occludin claudin-1 claudin-4 junctional adhesion molecule and ZO-1. (scienceexhibitions.org)
Assessed by Western blot analysis1
- The OCC2 peptide decreased the amount of occludin present at the tight junction, as assessed by indirect immunofluorescence, as well as decreased total cellular content of occludin, as assessed by Western blot analysis. (rupress.org)
Polyclonal Antibody2
- The following product was used in this experiment: ZO-2 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 71-1400, RRID AB_2533976. (thermofisher.com)
- This polyclonal antibody is produced by immunizing goats with purified occludin protein or a specific peptide sequence derived from occludin. (affigen.com)
Protein10
- Proximity ligation assay, immunoblotting, and phosphatase assay were performed to study the effect of DUSP3 on the TJ protein occludin (OCLN). (biomedcentral.com)
- Occludin, the putative tight junction integral membrane protein, is an attractive candidate for a protein that forms the actual sealing element of the tight junction. (rupress.org)
- The effect on occludin was specific because other tight junction components, ZO-1, ZO-2, cingulin, and the adherens junction protein E-cadherin, were unaltered by OCC2 treatment. (rupress.org)
- We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
- We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
- The AffiAB® Goat Anti-OCLN Polyclonal IgG Antibody is an antibody specifically designed to target and detect the occludin protein. (affigen.com)
- Occludin is a tight junction protein that plays a crucial role in the formation and maintenance of epithelial and endothelial cell barriers. (affigen.com)
- Occludin is a key component of tight junctions, which are specialized protein complexes that seal the gaps between adjacent cells, regulating the paracellular transport of ions, solutes, and macromolecules. (affigen.com)
- Since the protein plays a critical role in the virus life cycle, chemical compounds that block p7 function are potential candidates for anti-viral therapy. (monash.edu)
- Fp-EVs upregulated the protein expression of zona occludens (ZO)-1 and Occludin and increased the ratio of Tregs in the colon tissue of colitis mice. (biomedcentral.com)
OCLN6
- Immunofluorescence staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. (atlasantibodies.com)
- Data show that claudin-6 (CLDN6) R209Q and occludin (OCLN) P24A mutations do not affect HCV pseudoparticles (HCVpp) entry. (cusabio.com)
- Our AffiAB® Goat Anti-OCLN Polyclonal IgG Antibody is a highly specific, validated reagent excellent for Western blotting applications. (affigen.com)
- The AffiAB® Goat Anti-OCLN Polyclonal IgG Antibody is widely used in research applications to investigate the expression, distribution, and function of occludin in various biological systems. (affigen.com)
- It is important to note that the AffiAB® Goat Anti-OCLN Polyclonal IgG Antibody specifically targets occludin and may not cross-react with other proteins. (affigen.com)
- In summary, the AffiAB® Goat Anti-OCLN Polyclonal IgG Antibody is a valuable tool for studying the role of occludin in cell barrier function and related processes. (affigen.com)
Thermo Fisher Sci1
- The following product was used in this experiment: ZO-1 Monoclonal Antibody (ZO1-1A12) from Thermo Fisher Scientific, catalog # 33-9100, RRID AB_2533147. (thermofisher.com)
Epithelial tight junction1
- We assessed changes in the gut microbiota, intestinal permeability, gut peptides, intestinal epithelial tight-junction proteins ZO-1 and occludin (qPCR and immunohistochemistry), hepatic and systemic inflammation. (bmj.com)
Claudins1
- Cerebral microvascular endothelial cells are linked together through the interactions among multiple tight junction proteins including claudins, occludin and ZO-1 [ 5 ]. (researchsquare.com)
Antigen3
- This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
- This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- Nanobodies (~15 kDa), derived from heavy-chain-only antibodies of camelids, represent the smallest naturally occurring antigen binding reagent. (uni-tuebingen.de)
Immunohistochemistry4
- Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. (novusbio.com)
- Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. (novusbio.com)
- Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex. (novusbio.com)
- This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and immunoprecipitation to detect and analyze the expression and localization of occludin in different tissues and cell types. (affigen.com)
Endothelial cell1
- In this study, we investigated the effects of TNF-α and propofol on occludin expression in human cerebral microvascular endothelial cell line, D3 clone (hCMEC/D3 cells), and explored the underlying mechanisms. (researchsquare.com)
Rabbit polyclonal1
- After washing rabbit polyclonal anti-ICAM-1 was added for 2 h followed by horseradish peroxidase (HRP)-labeled secondary antibody. (scienceexhibitions.org)
Isotype1
- Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). (novusbio.com)
CD811
- Deletion of HVR1 increases CD81-binding ability of the mutant and also increases its susceptibility to neutralizing antibody MAb 24. (monash.edu)
Reactivity1
- In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
Barrier function2
- By targeting occludin, researchers can examine its role in epithelial and endothelial barrier function, cell signaling, cell migration, and other cellular processes. (affigen.com)
- A study revealed that truncation of occludin decreases transendothelial electrical resistance, suggesting a key role of occludin in the barrier function of the tight junction [ 8 ]. (researchsquare.com)
Physiological1
- It allows researchers to investigate the impact of occludin in various physiological and pathological conditions, including barrier dysfunction, inflammation, cancer metastasis, and infectious diseases. (affigen.com)
Tight8
- To study the role of occludin in the formation of the tight junction seal, synthetic peptides (OCC1 and OCC2) corresponding to the two putative extracellular domains of occludin were assayed for their ability to alter tight junctions in Xenopus kidney epithelial cell line A6. (rupress.org)
- The correlation between a decrease in occludin levels and the perturbation of the tight junction permeability barrier provides evidence for a role of occludin in the formation of the tight junction seal. (rupress.org)
- It also exacerbated BBB disruption and decreased the expression of tight junction-associated proteins, including ZO-1, claudin-5, and occludin-1, whereas the PPARγ agonist rosiglitazone (RSG) had the opposite effects. (researchsquare.com)
- Thereby, certain epithelial and mesenchymal proteins, including the tight junction component occludin, the actin cytoskeleton, the transcriptional repressor SNAI1 and the intermediate filament vimentin, serve as specific biomarkers or even as target structures for compounds. (uni-tuebingen.de)
- Purpose: The levels of tight junction proteins (TJs), especially occludin correlate with blood-brain barrier (BBB) disruption caused by central nervous system (CNS) inflammation during perioperative period. (researchsquare.com)
- The decreased expression of tight junction proteins, especially occludin, correlates with BBB disruption in several situations such as cerebral ischemic injury [ 7 ]. (researchsquare.com)
- In general, the BSCB is more permeable than the BBB, largely due to the relatively low expression of tight junction proteins like ZO-1 and occludin. (wikipedia.org)
- In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)
Hybridomas1
- Mab Aiib2 Anti Human Integrin β1 Hybridomas, supplied by ATCC, used in various techniques. (bioz.com)
Cellular1
- Pulse-labeling and metabolic chase analysis suggested that this decrease in occludin level could be attributed to an increase in turnover of cellular occludin rather than a decrease in occludin synthesis. (rupress.org)
Goat1
- After immunization, the antibody is purified from goat serum to ensure high specificity and quality. (affigen.com)
Biological2
- Hyperforin is a type of bicyclic tetraketone with four isoprenoid chains extracted from Hypericum perforatum L. that has multiple biological activities such as anti‑diabetes, antitumor and anti‑inflammation. (spandidos-publications.com)
- Specific nanobodies against occludin, SNAI1 and vimentin were selected via the phage display technology and respective binding properties of nanobodies and chromobodies were analyzed in biochemical and cell biological assays. (uni-tuebingen.de)
Decrease1
- It has been reported that TNF-α could decrease the expression of occludin in endothelial cells [ 13 ]. (researchsquare.com)
Specific1
- Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. (novusbio.com)
Thesis1
- In this thesis, novel single-domain antibodies (nanobodies) were selected to trace EMT biomarkers in a cancer-relevant living cell system. (uni-tuebingen.de)
Immunofluorescence2
- Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). (novusbio.com)
- Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. (novusbio.com)
Cells4
- In these studies, CD4 + T cell-depleting and blocking antibodies caused remission from disease in a number of CD and UC patients examined, suggesting a prominent role of CD4 + T cells in propagating disease ( 1 , 2 ). (frontiersin.org)
- Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. (novusbio.com)
- We tested if anti-human β1 mAb AIIB2 blocks β1-3-1-CHO cells adhesion to mCRP. (bioz.com)
- U937 cells were preincubated with antibodies (25 μg/ml) for 30 min at 37°C. After 4 h incubation, migrated cells were counted. (bioz.com)
Human2
- Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate. (novusbio.com)
- Antibodies used were mAb 7E3 (to human β3, 25 μg/ml), mAb SG73 (to human α4, 25 μg/ml), and AIIB2 (to human β1, 25 μg/ml). "mIgG" represents purified mouse IgG used as a control. (bioz.com)
Role1
- The intestinal epithelial barrier also has in important role in implementing an immune response with the gut-associated lymphoid tissue (GALT), through the initiation of pro- and anti-inflammatory signals [vi] . (layerorigin.com)
Inflammation1
- Propofol, a widely used intravenous anesthetic agents, has been reported to have anti-inflammation effects. (researchsquare.com)
Western2
- Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. (novusbio.com)
- Western blot was performed using Anti-ZO-1 Monoclonal Antibody (ZO1-1A12) (Product # 33-9100) and a 225 kDa band corresponding to ZO-1 was observed across along with uncharacterized band. (thermofisher.com)
Tissue1
- We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
Cell3
- Antibodies recognizing native CLDN6 as displayed on cell surfaces and mediating complement-dependent cytotoxicity were elicited in vaccinated animals. (cusabio.com)
- Finally, IL-17A neutralizing antibodies had been given to mice with energetic atrophic gastritis to judge the consequences on Levistilide A parietal cell atrophy and metaplasia. (nihvp.org)
- Finally, IL-17A neutralizing antibody reduced parietal cell atrophy and metaplasia in mice with chronic atrophic gastritis. (nihvp.org)
Study2
- In this study, we sought to explore whether propofol could attenuate the effect of TNF-α on occludin expression and identify the underlying mechanisms. (researchsquare.com)
- Our in vitro study revealed that ergosterol peroxide (EP) from the mushroom Cryptoporus volvatus has efficient anti-PDCoV properties. (biomedcentral.com)
Expression1
- Results: TNF-α (10 ng/ml, 4 h) significantly decreased the expression of occludin, which was attenuated by propofol (25 μM). (researchsquare.com)
Mouse1
- For JAM plates were coated overnight with 1 3 10 or 30 μg lysate and incubated with mouse monoclonal anti-JAM (3D8) for 2 h and visualized with antimouse HRP. (scienceexhibitions.org)
Compounds1
- Targeting EMT is of particular interest for the development of novel compounds in the anti-metastatic cancer therapy. (uni-tuebingen.de)