• All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. (sigmaaldrich.com)
  • Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • This effect was obtained with a polyclonal antibody as well as with one from 12 monoclonal antibodies raised against the native enzyme purified from chicken gizzard. (morulaivf.com)
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
  • The uniqueness and low cross-reactivity of the Prestige Antibodies ® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. (sigmaaldrich.com)
  • Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. (sigmaaldrich.com)
  • The best-characterized antigen is a 97-kd protein extracted from human epidermis that binds IgA antibodies from sera of patients with linear IgA dermatosis. (medscape.com)
  • this antigen is recognized by circulating antibodies in some patients with linear IgA dermatosis, but it has not been further characterized. (medscape.com)
  • Anti-double stranded DNA (Anti-dsDNA) antibodies are a group of anti-nuclear antibodies (ANA) the target antigen of which is double stranded DNA. (wikipedia.org)
  • These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)
  • We performed fluorescence overlay antigen mapping by laser scanning confocal microscopy (FOAM-LSCM) to identify autoantigens in an anti-p200 pemphigoid patient and assessed its usefulness for the diagnosis. (bvsalud.org)
  • however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
  • The invention relates to methods of modulating the antigen-binding affinity of an antibody by determining, using data corresponding to the structure of a complex between the antibody and an antigen in a solvent, a representation of a charge distribution of the CDRs of the antibody which minimizes electrostatic contribution to binding free energy between the antibody and the antigen in a solvent. (justia.com)
  • Guided by these determinations, the antibody is accordingly modified (altered) to improve upon, e.g., antibody/antigen binding by modifying at least one amino acid residue to decrease the binding free energy between the antibody and antigen when bound in a solvent. (justia.com)
  • Researchers hoped that such antibodies would be no more foreign to the human body than a genuine human antibody, but the utility of such antibodies has been restricted, at least in some cases, by a reduction in the antibody's affinity for the antigen. (justia.com)
  • Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band. (antibodiesinc.com)
  • Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ITGA1 antibody. (bio-connect.nl)
  • Western blot analysis of purified human recombinant Laminin-411, Laminin-421, Laminin-511, Laminin-121, Laminin-221 and Laminin-332. (atlasantibodies.com)
  • This antibody can also be used for the identification human beta-DG, mouse beta-DG and recombinant beta dystroglycan in a western blot. (absoluteantibody.com)
  • The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. (sigmaaldrich.com)
  • Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
  • This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. (lsbio.com)
  • Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. (biogenex.com)
  • The first evidence for antinuclear antibodies arose in 1948 when Hargraves, Richmond and Morton discovered the LE cell. (wikipedia.org)
  • Immunohistochemistry analysis in human prostate and skeletal muscle tissues using AMAb91461 antibody. (bio-connect.nl)
  • Immunohistochemistry analysis in human placenta and lymph node tissues using AMAb91133 antibody. (atlasantibodies.com)
  • This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. (absoluteantibody.com)
  • In cases in which type VII collagen is the molecule against which the antibody response is directed, patients are less likely to be responsive to treatment. (medscape.com)
  • Laminin and type IV collagen are the two principal components of the basement membrane. (biogenex.com)
  • Epidermolysis bullosa acquisita is caused by antibodies targeting type VII collagen, the major component of anchoring fibrils that connect the basement membrane to dermal structures. (medscape.com)
  • Various murine models have contributed to the understanding of the pathogenic role of antitype VII collagen antibodies and pathophysiology of epidermolysis bullosa acquisita. (medscape.com)
  • [ 7 ] The failure of rabbit antimouse type VII collagen antibodies to induce blisters in C5-deficient mice supports a role for complement activation in the disease pathogenesis. (medscape.com)
  • Prestige Antibodies ® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. (sigmaaldrich.com)
  • Because antibodies that bind the 97- and 120-kd antigens do not recognize the 180-kd BPAg2, the former may express unique epitopes distinct from those of the parent protein. (medscape.com)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • Immunoblot assays using human epidermal extracts and BP180 NC16a domain recombinant protein confirmed the presence of IgG antibodies against BP180 and recombinant BP180 NC16a domain protein. (morulaivf.com)
  • Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • Although the exact mechanism of the generation of dsDNA antibodies is still unknown, it is likely that extracellular DNA is one cause of an immune response against dsDNA. (wikipedia.org)
  • [ 4 ] Antilaminin 332 antibodies are typically seen in malignancy-associated mucous membrane pemphigoid (MMP). (medscape.com)
  • The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
  • Twenty-four hours after plating on laminin substrate, TG1N and TG16 cells adopted a bipolar and elongated shape (Supplementary Fig. 1 B) and displayed high motility (mean velocities of 26.3 ± 0.6 µm/h and 25.7 ± 1.1 µm/h, respectively) without a predefined direction (Supplementary Fig. S1 C, Supplementary Movies S1 and S2 ), consistently with random motility pattern with high velocity previously reported for other GSC lines 30 . (nature.com)
  • The original antibody was generated by immunizing Balb/c and Trianni mice with human beta-dystroglycan. (absoluteantibody.com)
  • Humanized version was generated by grafting CDRs of parental mouse antibody AS30 onto human framework regions. (absoluteantibody.com)
  • Accordingly, there remains a need for antibodies that do not provoke a strong immune response but yet bind strongly to their antigens and methods for identifying such antibodies. (justia.com)
  • Antibody deposition leads to complement activation and neutrophil chemotaxis, which eventuates in loss of adhesion at the dermal-epidermal junction and in blister formation. (medscape.com)
  • Clinical manifestation coincides with the employment of humoral immune mechanisms, enrolling plasma cell recruitment, deposition of myelin-bound IgG and antibody-dependent macrophageal myelin-stripping. (biomedcentral.com)
  • These agents have anti-inflammatory properties and cause profound and varied metabolic effects. (medscape.com)
  • Sulfasalazine elicits anti-inflammatory effects. (medscape.com)
  • Efficacy may be due to anti-inflammatory properties. (medscape.com)
  • FOAM-LSCM revealed that the patient's IgG signals were co-localized with laminin γ1 but were observed above type VII collagens. (bvsalud.org)
  • He had anti-TIF1γ antibody-positive dermatomyositis and was treated with prednisolone sodium succinate (20 mg/day). (bvsalud.org)
  • In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. (bmj.com)
  • Antibodies are exquisite, naturally occurring biological agents that play a critical role in defending the body from pathogens. (justia.com)
  • When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
  • Anti-dsDNA antibodies are incredibly specific for SLE, with studies quoting nearly 100%, and are therefore used in the diagnosis of SLE. (wikipedia.org)
  • The diagnosis of anti-p200 pemphigoid is occasionally difficult, mainly due to the lack of standardized tests. (bvsalud.org)
  • Higher titres of anti-dsDNA antibodies are more suggestive of SLE and lower titres can be found in people without the disease. (wikipedia.org)
  • Therefore, presence of anti-dsDNA antibodies are suggestive of SLE, however an absence of the antibodies does not rule out the disease. (wikipedia.org)
  • The levels of circulating anti-dsDNA antibodies fluctuate with disease activity in SLE. (wikipedia.org)
  • Increases in titres of the antibodies can coincide with, or even precede an increase of disease activity. (wikipedia.org)
  • The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis. (bmj.com)
  • Anti-dsDNA antibodies can also be produced through infection via a mechanism known as molecular mimicry. (wikipedia.org)
  • Antibodies, which are also commonly referred to as immunoglobulins, contain four polypeptides: two longer polypeptides ("heavy chains") that are identical to one another and two shorter polypeptides ("light chains") that are identical to one another. (justia.com)
  • Upon exposure to pneumococcal polysaccharides, cross reactive antibodies between dsDNA and pneumococcal polysaccharides are produced in lupus. (wikipedia.org)