PolyclonalMAPK1Erk1 and Erk2Affinity PurifiedFITCMonoclonal antibodiesImmunofluorescenceWestern BlotQuality antibodiesRabbitImmunogenELISAKinaseProteinRecombinantPathwaysCetuximabPhosphorylationActinProteinsAntigenIncubationInhibitionIgG1ProductsCellsHumanPrimaryMouseLysatesProductCellExpressionNoteEffect
Polyclonal4
- ERK1 antibody LS-C16352 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (pThr202/Tyr204) from human. (lsbio.com)
- Description: A polyclonal antibody for detection of CSP from Human, Mouse, Rat. (phylocommons.org)
- Description: A Rabbit Polyclonal antibody against CSP from Human/Mouse/Rat. (phylocommons.org)
- Description: A polyclonal antibody raised in Goat that recognizes and binds to Human DNAJC5 / CSP (aa177-191). (phylocommons.org)
MAPK13
- Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. (arigobio.com)
- B ) Feature plots showing the expression of Braf, Map2k1 (MEK1), Map2k2 (MEK2), Mapk3 (ERK1), Mapk1 (ERK2), Ksr1, and Ksr2 genes for the same cells in (A). ( C ) Violin plots showing median gene expression from (B) across cell-type clusters. (bioz.com)
- Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. (antibodies-online.cn)
Erk1 and Erk22
- Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). (lsbio.com)
- Designed as an oral medication, ERAS-007 exerts its anti-cancer effect by targeting and inhibiting the ERK1 and ERK2 signaling proteins, which disables the RAS/MAPK pathway, a central cascade in cancer. (pharmalive.com)
Affinity Purified1
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (cusabio.com)
FITC2
- Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat anti Human TNF alpha ( MCA1560PE ). (bio-rad-antibodies.com)
- Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A ( BUF077A ) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 ( MCA463F ) and PE conjugated Rat IgG1 ( MCA6004PE ). (bio-rad-antibodies.com)
Monoclonal antibodies3
- Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
- Indeed, DPPI deficient mice were protected against acute arthritis induced by passive transfer of monoclonal antibodies against type II collagen (Adkison et al. (justia.com)
- Monoclonal antibodies have been then purified to homogeneity working with regular approaches just before storage at 1 mg ml or 0. (liverxreceptor.com)
Immunofluorescence2
- Immunocytochemistry/ Immunofluorescence: ZCCHV Antibody [NBP2-20940] - ZCCHV antibody detects ZCCHV protein at cytoplasm by immunofluorescent analysis. (novusbio.com)
- For immunofluorescence, we used antibodies in opposition to PDGFRα and different markers of ICs. (aetox.com)
Western Blot3
- Western blot was performed using Anti-ZO-1 Monoclonal Antibody (ZO1-1A12) (Product # 33-9100) and a 225 kDa band corresponding to ZO-1 was observed across along with uncharacterized band. (thermofisher.com)
- Western Blot: ZCCHV Antibody [NBP2-20940] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZCCHV diluted at 1:10000. (novusbio.com)
- Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). (novusbio.com)
Quality antibodies2
- Non-specific binding caused by low quality antibodies is reduced while signal from the specific antibody-antigen complex is stabilized and enhanced. (scienceimaging.se)
- High quality antibodies with extensive validation data. (antibodies-online.cn)
Rabbit1
- Membranes have been incubated with a hundred ng ml of MOAB 2 or IgG2b isotype matched management and then rabbit anti mouse antibody conjugated with HRP and visualized with ECL substrate. (liverxreceptor.com)
Immunogen2
ELISA1
- This CSP antibody is for WB, ELISA. (phylocommons.org)
Kinase2
- Curcumin-induced paraptosis has been reported to be positively associated with ERK2 and JNK (c-jun N-terminal kinase-1) activation [ 16 ]. (hindawi.com)
- Furthermore, in tumour necrosis factor alpha (TNFa)-stimulated Ewing's sarcoma (ES) cells, NF-jB is reported to prevent activation of an apoptotic cascade through inhibition of c-Jun N-terminal kinase (JNK) [23] and upregulation of anti-oxidant enzymes [24]. (lsd1receptor.com)
Protein6
- Immunoblot analyses with anti-active mitogen-activated protein kinases (MAP-Ks) revealed that D1 receptors, D2 receptors, group I mGluRs, and group II mGluRs all contribute to MAP-K activation in prefrontal cortex, and that combined activation of dopamine receptors and mGluRs synergistically or additively activate MAP-Ks. (jneurosci.org)
- Immunohistochemistry-Paraffin: ZCCHV Antibody [NBP2-20940] - ZCCHV antibody detects ZCCHV protein at cytoplasm by immunohistochemical analysis. (novusbio.com)
- Immunohistochemistry-Paraffin: ZCCHV Antibody [NBP2-20940] - ZCCHV antibody detects ZCCHV protein at cytoplasm in human lung cancer by immunohistochemical analysis. (novusbio.com)
- This complex behavioral profile was accompanied by decreases in ERK2 mRNA and protein phosphorylation within the VTA, while stress alone resulted in opposite neurobiological effects. (jneurosci.org)
- A) Averaged outcomes of reverse-phase antibody array evaluation of 46 signalling protein in A431 cells stably expressing shDUSP6, shDUSP7, or non-silencing shRNA control. (synanet2020.com)
- A different approach to focusing on the same protein-protein connection (PPI) interface was carried out by Quevedo and colleagues [138] (Number 4H) using an intracellular anti-mutant Ras antibody fragment like a competitor inside a small-molecule library screen for identifying Ras-binding compounds. (moonphase2018.com)
Recombinant1
- Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. (abcam.com)
Pathways2
- CCL2 induces amylin expression through ERK1/ERK2/JNK-AP1 and NF-κB related signaling pathways independent of CCR2. (wikipedia.org)
- MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. (abcam.com)
Cetuximab3
- In April, at the annual meeting of the American Association for Cancer Research, Erasca presented promising preclinical data supporting the strong anti-cancer activity of ERAS-007 as a monotherapy and in combination with Braftovi or established cancer drugs such as cetuximab. (pharmalive.com)
- Earlier this year, Erasca announced a clinical trial collaboration and supply agreement with Eli Lilly to evaluate another asset, ERAS-601, an oral SHP2 inhibitor, with Lilly's anti-EGFR antibody cetuximab. (pharmalive.com)
- However, little is known about the role of the rarer NRAS mutations as a mechanism of primary resistance to the anti-EGFR monoclonal antibody cetuximab in wild-type KRAS mCRC. (oncotarget.com)
Phosphorylation3
- MLSA significantly curtailed the phosphorylation of ERK1/ERK2, stimulated by both PMA and anti-CD3 antibodies in Jurkat T cells. (bvsalud.org)
- Our finding demonstrates that Ca2+ influx via CRAC channels, inhibition of ERK1/ERK2 phosphorylation and IL-2 gene transcription may be implicated in immunosuppressive effects of MLSA antigen. (bvsalud.org)
- In spite of the truth that our data suggest that MMPs are usually not expected, we confirmed a requirement for HB-EGF to market E2- and G-1-induced, GPER-mediated phosphorylation of ERK and proliferation each by sequestering and down-modulating proHB-EGF with CRM-197 and by blocking its ability to bind EGFR with neutralizing antibodies. (igf-1r.com)
Actin1
- Samples were separated by Phos-tag PAGE (top panel) and SDS-PAGE (middle and bottom panels) and analyzed by immunoblotting with antibodies to Pex14 and actin, a loading control. (elifesciences.org)
Proteins2
- Consistently, quercetin reduced the steady-state levels of K-, H-, and NRas mRNAs and proteins in both ERK2 colon cancer cell lines and main colorectal tumors [156]. (moonphase2018.com)
- Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. (nih.gov)
Antigen3
- This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- AdvanBlock™-Chemi is a novel blocking solution optimized to enhance specific antibody-antigen interactions for the best results in chemiluminescent Western Blots. (scienceimaging.se)
- Preliminary clonal assortment was performed by antigen antibody blotting. (liverxreceptor.com)
Incubation2
- This all-in-one blocking and antibody incubation solution is designed to improve sensitivity and decrease overall background. (scienceimaging.se)
- Immediately after a 3 × 5 min washing step with 1x PBS, incubation using the second antibody coupled to Alexa488, Alexa568 or Alexa647 for 1 h followed. (liverxreceptor.com)
Inhibition2
- We not too long ago printed that inhibition of the interplay between podoplanin-expressing cells and podoplanin-binding cells utilizing podoplanin-neutralizing antibodies reduces however doesn't totally suppress irritation post-MI whereas enhancing coronary heart operate and scar composition after ischemic harm. (aetox.com)
- Preclinical evidence supports synergistic anti-tumor effects when downstream RAS/MAPK pathway inhibition is combined with cell cycle inhibition in CRC and PDAC," he said. (pharmalive.com)
IgG11
- Source of antibodies For the techniques used in this review, the next primary antibodies had been utilized, full report MOAB two, IgG2b, 6E10 anti Ab residues three 8, mouse IgG1, 0. (liverxreceptor.com)
Products1
- antibodies-online.com stands for high-quality research products. (antibodies-online.cn)
Cells7
- Administration of anti-CCL2 antibodies in a model of glomerulonephritis reduces infiltration of macrophages and T cells, reduces crescent formation, as well as scarring and renal impairment. (wikipedia.org)
- Furthermore, MLSA failed to potentiate both thapsigargin- and anti-CD3 antibodies-induced capacitative calcium influx in Jurkat T cells. (bvsalud.org)
- In order to mimic co-stimulation of T cells, we stimulated them by both PMA and anti-CD3 antibodies. (bvsalud.org)
- Also MLSA was found to reduce the transcription of IL-2 gene in PMA plus anti-CD3 antibodies-activated Jurkat T cells. (bvsalud.org)
- Contrary to cells treated with a siRNA, cells treated with siRNAs against Bora often displayed multipolar spindles in mitosis, a phenotype that is also seen upon TPX2 RNAi Geneticin distributor and after injection of antibodies blocking Aurora A function. (others-signal.com)
- 5% cold fish gelatine and 0,1% ovalbumin 1x PBS for thirty min at RT plus the cells have been washed again 3 × 5 min with 1x PBS at RT, followed by the major antibody at four C overnight. (liverxreceptor.com)
- After the treatment with mock or 0.2 mM H 2 O 2 for 0.5 hr and 1 hr, Fao cells were incubated with vehicle (-) and λ-phosphatase (+) as in A. The cell lysates were analyzed by Phos-tag PAGE (upper panels), SDS-PAGE (lower panels), and immunoblotting with anti-Pex14 antibody. (elifesciences.org)
Human1
- Cell-free human cytomegalovirus (HCMV) may be inhibited by a soluble type of the mobile HCMV-receptor PDGFRα , resembling neutralization by antibodies . (aetox.com)
Primary2
- Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)
- Anti-ZKSCAN5 (SAB4501021), anti-VEGFC (ab83905), and anti-LYVE1 (ab10278) primary antibodies were used at 1:100, 1:100, and 1:50 dilutions, respectively. (bms-911543.com)
Mouse2
- Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. (novusbio.com)
- B ) Lysates of various mouse tissues (15 µg each) indicated at the top were analyzed by Phos-tag PAGE (upper panel), SDS-PAGE (lower panel), and immunoblotting with anti-Pex14 antibody. (elifesciences.org)
Lysates3
- HeLa whole cell lysates were subjected to Western blotting with an anti-phospho-STAT3 antibody. (scienceimaging.se)
- Cell lysates were analyzed as in A with antibodies to Pex14, phosphorylated Pex14 at Ser232 (Pex14-pS232), and lactate dehydrogenase (LDH). (elifesciences.org)
- Cell lysates were analyzed by SDS-PAGE (upper panel), Phos-tag PAGE (lower panels), and immunoblotting with indicated antibodies. (elifesciences.org)
Product1
- The following product was used in this experiment: ZO-1 Monoclonal Antibody (ZO1-1A12) from Thermo Fisher Scientific, catalog # 33-9100, RRID AB_2533147. (thermofisher.com)
Cell3
- They frequently play an anti-apopto-tic role [14-16], but may also be pro-apoptotic in some cell types and conditions [16-19]. (lsd1receptor.com)
- Anti P Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. (bioz.com)
- The cell-associated development of current HCMV isolates, nevertheless, is resistant in opposition to antibodies . (aetox.com)
Expression1
- Antibodies have been isotyped plus the stable clones adapted to serum free of charge medium and placed within a bioreactor for antibody expression. (liverxreceptor.com)
Note1
- Note that antibody to phsopho-Pex14 at Ser232 specifically recognized slower-migrating bands of Pex14 in Phos-tag PAGE. (elifesciences.org)
Effect1
- Little effect of H 2 O 2 treatment on Pex14 level and evaluation of Pex14 variants with anti-Pex14-pS232 antibody. (elifesciences.org)