• Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram). (biolegend.com)
  • Anti-CD81 PE (Clone M38) as primary antibody detector. (immunostep.com)
  • CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. (biolegend.com)
  • HIV gag proteins use tetraspanin enriched microdomains (containing minimally CD81, CD82, CD63) as a platform for virion assembly and release. (wikipedia.org)
  • Anti-CD81 antibodies downregulate HIV production 3 fold, however the CD81 protein free virus is more infectious. (wikipedia.org)
  • Co-immunoprecipitation experiments showed that viral Gag proteins interact, directly or indirectly, with the CD81 tetraspanin, and less with CD82, but not with Lamp2 or CD45, in tetraspanin-enriched microdomains composed of CD81/CD82/CD63. (biomedcentral.com)
  • When HIV-1 producing cells were treated with anti-CD81 antibodies or upon CD81 silencing by RNA interference, HIV-1 release was significantly impaired and its infectivity on SupT1 lymphocytes was modulated. (biomedcentral.com)
  • Furthermore, EVs isolated with anti-CD61 beads enclosed mRNA expression patterns that might be associated to early-stage lung cancer, in contrast with EVs captured through CD9, CD63 or CD81. (biomedcentral.com)
  • Figure A. A647 conjugated mouse anti rat CD63 ( MCA4754A647 ) and FITC conjugated mouse IgG1 isotype control ( MCA1209F ). (bio-rad-antibodies.com)
  • Staining of the TE-71 cell line with 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (blue histogram) or 0.06 µg of Anti-Mouse CD326 (EpCAM) APC (purple histogram). (thermofisher.com)
  • The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. (biolegend.com)
  • The following product was used in this experiment: TIMP1 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 16644-1-AP. (thermofisher.com)
  • Affinity purified rabbit polyclonal antibody reactive to Zaire Ebola virus L-Polymerase. (receptors.org)
  • Despite their efficiency, adoptive T-cell therapies show unique toxicities, which are distinct from those seen with conventional chemotherapies, monoclonal antibodies (mAbs), and small-molecule-targeted therapies. (nature.com)
  • Diaclone Monoclonal Antibodies are highly specific, high quality mouse monoclonal antibodies for the detection of a broad range of human proteins. (diaclone.com)
  • MORE THAN 98% of Diaclone Monoclonal antibodies are produced in vitro ! (diaclone.com)
  • Diaclone now offers an extensive selection of monoclonal antibodies produced in cell culture and preservative free. (diaclone.com)
  • Diaclone is working to supplement the large portfolio of currently available monoclonal antibodies and meet the increased demand for the development of antibodies for new targets of interest. (diaclone.com)
  • CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). (biolegend.com)
  • Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. (thermofisher.com)
  • CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. (biolegend.com)
  • The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. (neobiotechnologies.com)
  • Figure B. A647 conjugated mouse anti rat CD63 ( MCA4754A647 ) and FITC conjugated mouse anti rat CD61 ( MCA1773F ). (bio-rad-antibodies.com)
  • The exosome marker CD63 was primarily localized to the apices of principal cells throughout the epididymal epithelium. (oncotarget.com)
  • The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. (immunostep.com)
  • Blocking exosome secretion using the sphingomyelinase inhibitor GW4869 not only increased cortical bone volume, but also it sensitized the myeloma cells to bortezomib, leading to a strong anti-tumor response when GW4869 and bortezomib were combined. (evtrack.org)
  • Activated phospho-kinases involved in exosome-treated adipocytes were detected by phospho-kinase antibody array and Western blot. (biomedcentral.com)
  • CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. (biolegend.com)
  • CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. (neobiotechnologies.com)
  • Diaclone supplies both in vitro and in vivo produced monoclonal and biologically active antibodies, depending on which method generates the best quality, specificity and reactivity for successful and reproducible results. (diaclone.com)
  • Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)
  • Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. (thermofisher.com)
  • It is also known as 26 kDa cell surface protein, TAPA-1 (Target of the Antiproliferative Antibody 1), and Tetraspanin-28 (Tspan-28). (wikipedia.org)
  • Analysis of Protein Array containing more than 19,000 full-length human proteins using CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (neobiotechnologies.com)
  • This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. (neobiotechnologies.com)
  • Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
  • Western blot detection was performed to determine the cross-reactivity of the antibody to virus like particles (VLPs) of Zaire and Sudan ebola virus (ZEBOV and SEBOV) and Marburg virus (MARV) expressing glycoprotein, nucleoprotein and VP40. (receptors.org)
  • For the characterization of exosomes, micro vesicles (MV) and virions, resumed as bio-nanoparticles (BNPs), fluorescent staining techniques allow fast and specific staining of membranes, surface proteins or nucleic acids by applying lipid dyes, antibodies, and intercalating RNA/DNA dyes [1, 2]. (trillium.de)
  • A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. (rndsystems.com)
  • Why Use an Antibody Array to Detect Angiogenesis-Related Proteins? (rndsystems.com)
  • The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample. (rndsystems.com)
  • Mouse anti Rat CD63 antibody 6H1 ( MCA4754GA ) used to demonstrate CD63 expressing cells by immunofluorescence. (bio-rad-antibodies.com)
  • a Immunofluorescence of CD63 (green) and mTOR (red) in INS1 cells treated with control (1% BSA, 11 mM glucose, "1% BSA") or glucolipotoxic (1% BSA, 33.3 mM Glucose and 0.4 mM Palmitate, "Glc/Pal") media for 20 h. (bio-rad-antibodies.com)
  • The following product was used in this experiment: CD326 (EpCAM) Monoclonal Antibody (G8.8), APC, eBioscience™ from Thermo Fisher Scientific, catalog # 17-5791-82, RRID AB_2716944. (thermofisher.com)
  • Beads with covalently-conjugated antibodies on their surface outperformed streptavidin-conjugated beads, coated with biotinylated antibodies, in EV immunoprecipitation. (biomedcentral.com)
  • CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. (neobiotechnologies.com)
  • Introducing a new blocking antibody targeting complement C5, now available for preclinical research! (biolynx.ca)
  • 500 µg of affinity purified antibody is provided in PBS at a concentration of 0.93 mg/mL. 0.01% Sodium azide has been added. (receptors.org)
  • But that's not all - Hycult also has new antibodies to target complement C7 and clusterin, two molecules associated with the formation of the TCC/MAC-complex. (biolynx.ca)
  • CDE can also send anti or pro-tumor signals to immune and nonimmune cells, thereby altering their functions. (encyclopedia.pub)
  • Our expertise and know-how make us a trusted partner for custom antibody development & bulk antibody supply. (diaclone.com)
  • Utilize our scientific expertise in antibody engineering and development. (diaclone.com)
  • We have the ability to generate new biologically active antibodies by using our Bioassays platform. (diaclone.com)
  • In current clinical development, targeting CD19 + B cell malignancies, which include acute and chronic B-cell leukaemia and B-cell non-Hodgkin lymphomas (NHLs), with anti-CD19 CAR-T cells is one of the most advanced adoptive T-cell therapies and has been approved by the FDA. (nature.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • The antibody detects L-Polymerase by Western blot in ZEBOV infected HeLa cells. (receptors.org)
  • With over 30 years of experience Diaclone is respected for its excellence in Monoclonal Antibody development and our highly respected B-clones ('B-' for Besancon) are utilized across the Life Science industry in research, clinical and discovery applications by research, pharmaceutical and manufacturing organizations worldwide. (diaclone.com)
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (thermofisher.com)
  • Retinoids, compounds derived from vitamin A, have a history of clinical use in cancer for their anti-proliferative and differentiation effects, and more recently have been explored as anti-stromal therapies in PDAC for their ability to induce mechanical quiescence in cancer associated fibroblasts. (bvsalud.org)
  • Assuming an EV of 100 nm and an antibody with 3 nm in size, the number of antibodies conjugated to an EV is ~4000 for a monolayer. (trillium.de)
  • our results identify the CD63-syntenin-1-ALIX complex as a key regulatory component in post-endocytic HPV trafficking. (cusabio.com)
  • Altogether, our results indicate an important role for exosomes in the BM microenvironment and suggest a novel therapeutic target for anti-myeloma therapy. (evtrack.org)
  • Presumably an average of 2.5 fluorochromes per antibody results in ~10000 fluorochromes attached to an EV. (trillium.de)
  • See Products details for the concerned reference. (diaclone.com)
  • The in vivo monoclonal antibody production process (Ascites production) is recognised as being painful for animals. (diaclone.com)
  • Only a few monoclonal antibody clones with better specificity & productivity from ascites production have been conserved in the catalogue, in accordance with the strict regulations of veterinary agencies. (diaclone.com)
  • This antibody is available for research use only and is not approved for use in diagnosis. (neobiotechnologies.com)
  • Biologically active antibodies: Unable to find a neutralizing antibody for your research? (diaclone.com)
  • Unable to find an antibody for your research? (diaclone.com)
  • In contrast, miR-378c depletion decreased the anti-fibrotic effects of Pd-MSCs-EVs. (biomedcentral.com)
  • SDS-PAGE Analysis Purified CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5). (neobiotechnologies.com)