• All vaccine preparations were conceived to elicit anti-Spike protein immune responses, and their effectiveness relies on the generation of neutralizing antibodies. (nature.com)
  • Administration of anti-SARS-CoV-2 mRNA-based vaccines leads to the production of extraordinarily high levels of anti-Spike antibodies in serum 1 , 2 . (nature.com)
  • Monoclonal antibodies are commonly utilized as anti-inflammatory, anti-cancer and anti-viral therapeutic agents. (news-medical.net)
  • Commercially available test antibodies included CD71 clone 1a, 2, CD20 (Sigma), clone 3-5 (Abcam), CD3, CD45 (Biolegend), human IgG isotype control (Absolute Antibody), mouse IgG1 isotype control (R&D Systems). (news-medical.net)
  • 3. Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O. Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule. (southernbiotech.com)
  • Dosimetry with 188 Re-labelled monoclonal anti-CD66 antibodies. (uniklinik-ulm.de)
  • The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. (bdbiosciences.com)
  • CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. (bdbiosciences.com)
  • The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
  • Combinations of CD45 isoforms are crucial for immune function and disease. (exbio.cz)
  • Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. (nsjbio.com)
  • This antibody will bind to all CD45 isoforms. (nsjbio.com)
  • CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. (cytekbio.com)
  • The leucocyte-specific phosphatase CD45 is present in two main isoforms: the large CD45RA and the short CD45RO. (crcm-marseille.fr)
  • For example, co-expression of both isoforms identifies in vivo the anti tumor NK cells in hematological cancer patients. (crcm-marseille.fr)
  • The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). (cytekbio.com)
  • The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells. (cytekbio.com)
  • The purpose of this study was to determine if labeling leukocytes with a monoclonal antibody specific to leukocyte common antigen (CD45) was superior to labeling with LDS-751 and to determine the effect of sample fixation on a mouse whole blood preparation for flow cytometry. (arizona.edu)
  • We conclude that labeling leukocytes with monoclonal antibody CD45 in a mouse whole blood preparation is preferable, as it provides improved measurement of leukocyte indices compared to LDS-751. (arizona.edu)
  • The studies evaluated the feasibility of conjugating or labeling accelerator produced Ac-225 to lintuzumab, an anti-CD33 monoclonal antibody used in Actinium's CD33 ARC program, and the impact on ARC generation. (prnewswire.com)
  • Anti-CD45 monoclonal antibody YAML568: A promising radioimmunoconjugate for targeted therapy of acute leukemia. (uniklinik-ulm.de)
  • CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (abeomics.com)
  • CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (nsjbio.com)
  • CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. (biolegend.com)
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (exbio.cz)
  • With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. (cytekbio.com)
  • Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections 9 , inhibition of CD45 functions 4 , immunofluorescence 11 , Western blotting 3 , and spatial biology (IBEX) 16,17 . (biolegend.com)
  • Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Ab (2B11). (abeomics.com)
  • Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using CD45 (D3F8Q) Rabbit mAb performed on the Leica ® BOND ™ Rx. (cellsignal.com)
  • CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. (bdbiosciences.com)
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (exbio.cz)
  • Here, we describe CAR T cells targeting tumor-associated glycoprotein-72 (TAG72), utilizing the CD28 transmembrane domain upstream of the 4-1BB co-stimulatory domain as a driver of potent anti-tumor activity and IFNγ secretion. (bvsalud.org)
  • CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. (biolegend.com)
  • Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. (abeomics.com)
  • CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. (biolegend.com)
  • Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram). (biolegend.com)
  • CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. (biolegend.com)
  • BD Tritest™ CD3 fluorescein isothiocyanate (FITC)/CD4 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP) is a three-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3 + ) and helper/inducer (CD3 + CD4 + ) T-lymphocytes subsets in erythrocyte-lysed whole blood. (bdbiosciences.com)
  • B. burgdorferi infection in patients with cutaneous mani- atypical mitosis, B-lymphocytes (anti-CD20, DAKO, Car- festations suggestive of Lyme disease in Mexico. (cdc.gov)
  • Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
  • CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). (bdbiosciences.com)
  • Immunoprecipitation of CD45 protein from BaF3 cell extracts. (cellsignal.com)
  • Recently, they published an article in Science Translational Medicine about how CAR-T cells can target a protein on the surface of nearly all blood cancer cells named CD45. (thedp.com)
  • IHC testing of FFPE human tonsil with anti-CD45 antibody (clone SPM570). (nsjbio.com)
  • The samples were then fixed with paraformaldehyde or diluted with PBS followed by incubation with 5 μg/ml PerCP-conjugated anti-CD45, 5 μg/ml FITC-conjugated anti-CD11b, or 80 μM dichlorofluorescein diacetate. (arizona.edu)
  • Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is CD45 (D3F8Q) Rabbit mAb. (cellsignal.com)
  • Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)
  • Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. (exbio.cz)
  • Neoplastic cells from a T cell lymphoma were used as the immunogen for the anti-CD45 antibody. (nsjbio.com)
  • Confocal immunofluorescent analysis of Raw 264.7 (left, positive) and C2C12 (right, negative) cells using CD45 (D3F8Q) Rabbit mAb (green). (cellsignal.com)
  • Using cell cultures and mouse models, the study showed that anti-CD45 CAR-T cells are able to eliminate acute myeloid leukemia cells in a very short time span of roughly three weeks. (thedp.com)
  • Since CD45 is not only found in blood cancer cells but also in healthy blood cells, the researchers had to use a method called "epitope editing" to overcome the healthy blood cells. (thedp.com)
  • The CAR-T cells then bind to this small piece of the CD45 molecule and attack it. (thedp.com)
  • In contrast, the α-SMA(+) human TFPI(+) CD34(+) cells recruited in Tg recipients were from a CD45(-) lineage. (nih.gov)
  • Here we show that low CD45 expression associates with less mature, CD56bright, NK cells. (crcm-marseille.fr)
  • We analyzed infectivity, replication, cytotoxicity, CD107α upregulation of CD8+ and CD4+ T cells, CD274 (PD-L1) blockade of cancer cells by virus-encoded anti-PD-L1 scFv, and the release of growth factors and cytokines. (bvsalud.org)
  • We observed higher CD45-/large-size cells and lower CD8+ T cell percentages in MA than PW. (bvsalud.org)
  • CD45-/large-size cells were morphologically malignant and expressed CD274 (PD-L1), CD252 (OX40L), and EGFR. (bvsalud.org)
  • CF33-hNIS-antiPDL1 treatment produced functional anti-PD-L1 scFv, which blocked surface PD-L1 binding of live cancer cells and increased CD8+CD107α+ and CD4+CD107α+ T cell percentages while decreasing EGF, PDGF, soluble anti-PD-L1, and IL-10. (bvsalud.org)
  • Locoregional administration of mbIL12-engineered CAR T cells promotes durable anti-tumor responses against both regional and systemic disease in mice. (bvsalud.org)
  • The leaflets accumulated α-SMA and CD45 positive cells, with surfaces covered with endothelial cells (CD31+). (bvsalud.org)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)
  • Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • Chimeric antigen receptor (CAR) T cell therapeutic responses are hampered by limited T cell trafficking, persistence, and durable anti-tumor activity in solid tumors. (bvsalud.org)
  • B) Immunohistochemical image with anti-CD45 Ro antibody showing. (cdc.gov)
  • Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. (exbio.cz)
  • Epitope editing changes a small portion of the CD45 molecule by using CRISPR editing technology. (thedp.com)
  • CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. (biolegend.com)
  • CTCs in the blood sample are enriched by negative selection using anti-CD45 immunomagnetic beads. (medscape.com)
  • Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary. (cellsignal.com)
  • Confocal immunofluorescent analysis of wild-type mouse retina and optic nerve labeled with CD45 (D3F8Q) Rabbit mAb #70257 (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red). (cellsignal.com)
  • Confocal immunofluorescent analysis of mouse colon (left) and mouse kidney (right) using CD45 (D3F8Q) Rabbit mAb (green). (cellsignal.com)
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD62L-APC (SB Cat. (southernbiotech.com)
  • No. 1705-11) and Rat Anti-Mouse CD3ε-FITC (SB Cat. (southernbiotech.com)
  • AF647 Anti-human CD45 Ant. (bio-connect.nl)
  • Visualization of the human immunoglobulins specifically bound to HIV-1 proteins is accomplished in situ by using a series of reactions with goat anti-human IgG conjugated with biotin, avidin conjugated with horseradish peroxidase (HRP), and the HRP substrate 4-chloro-1-naphthol. (cdc.gov)
  • The EpCAM(+)/CD45(−) immunophenotype was defined as the metastatic involvement of carcinoma in the serous cavity. (karger.com)
  • 2021 ). They exert multiple biological functions, including limiting excessive inflammatory responses, regulating metabolism and immune cell functions, decreasing the production of pro-inflammatory factors, increasing anti-inflammatory mediators, and promoting tissue repair and homeostasis (Miao et al. (springer.com)
  • Background Anti(α)-programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) monotherapy fails to provide durable clinical benefit for most patients with carcinoma. (bmj.com)
  • The PECUU scaffolds had histologic evidence of scaffold degradation and an accumulation of pro-inflammatory/M1 and anti-inflammatory/M2 macrophages over time in vivo. (bvsalud.org)
  • Western blot analysis was performed using CD45 (D4H7K) Rabbit mAb #72787. (cellsignal.com)
  • We found that samples labeled with LDS-751 demonstrated decreased fluorescence intensity for granulocyte CD11b expression and ROS production compared to samples labeled with anti-CD45. (arizona.edu)
  • The expression of the following genes was salient: (a) Cd163, an anti-inflammatory stroke-associated gene, was the most differentially expressed gene by NPD1+RvD1, displaying more than a 123-fold upregulation in the ipsilesional penumbra (Lisi et al. (springer.com)
  • Spite and Serhan 2010 ), including Neuroprotectin D1 (NPD1) and D-series resolvins (RvD1), which display potent anti-inflammatory and immunoregulatory properties (Asatryan and Bazan 2017 ). (springer.com)
  • Overall, our study offers a novel conceptual framework for biomimetic NPs using a DoE strategy and consolidates the high therapeutic potential of leukosomes as a viable drug delivery system for anti-inflammatory and antineoplastic applications. (frontiersin.org)
  • A subsequent purging step was employed to remove contaminating WBCs through negative selection acoustophoresis with anti-CD45-functionalized negative acoustic contrast particles. (lu.se)
  • In addition, anti-Spike immunity in lungs of vaccinees was found either barely detectable or absent 8 . (nature.com)
  • BALB/c aortas (H-2(d)) transplanted into α-TFPI-transgenic (Tg) mice (H-2(b)) regenerated a quiescent endothelium in contrast to progressive IH seen in C57BL/6 wild-type (WT) mice even though both developed aggressive anti-H-2(d) alloresponses, indicating similar vascular injuries. (nih.gov)
  • In this scenario, the identification of new anti-SARS-CoV-2 vaccines eliciting adequate antiviral immunity in lungs would be of outmost relevance. (nature.com)
  • Maximal intrapulmonary recruitment of CD45(+)Col I(+)CXCR4(+) fibrocytes directly correlated with increased collagen deposition in the lungs. (wikigenes.org)