Anti cd11b antibody antigens cd11b. Medical search. Frequent questions

C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6c (GR-1) Pacific Blue™ and CD11b (clone M1/70) Brilliant Violet 750™ (left) or Ly-6G/Ly-6C (Gr-1) Pacific Blue™ only (right). (biolegend.com)
Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 ( MCA551 ) High power. (bio-rad-antibodies.com)

CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. (biolegend.com)
Macrophage-1 antigen (or integrin αMβ2 or macrophage integrin or Mac-1) is a complement receptor ("CR3") consisting of CD11b (integrin αM) and CD18 (integrin β2). (wikipedia.org)
Macrophage-1 antigen (hereafter complement receptor 3 or CR3) (CD11b/CD18) is a human cell surface receptor found on B and T lymphocytes, polymorphonuclear leukocytes (mostly neutrophils), NK cells, and mononuclear phagocytes like macrophages. (wikipedia.org)
CR3 CD11b/CD18 Macrophage 1 antigen (Mac-1) Macrophage Todd R (1996). (wikipedia.org)
CD18 non-covalently associates with CD11a, CD11b or CD11c. (angioproteomie.com)
CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). (exbio.cz)
expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. (abeomics.com)
CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. (abeomics.com)
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. (abeomics.com)
integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). (cytekbio.com)
CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. (biolegend.com)
CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. (biolegend.com)
The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). (cytekbio.com)

The RMP1-14 monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279. (bioxcell.com)
Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). (thermofisher.com)

BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry. (cytekbio.com)

Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)
Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)

The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. (cytekbio.com)
This antibody has been reported to stain granulocytes and macrophages in tissues such as rat spleen and thymus. (bdbiosciences.com)
In tumors which had high levels of infiltrating CD11b + macrophages expressing the ADCC-activating FcgRIV, T REGS were selectively depleted in an FcgR-dependant manner, while effector T cells were instead expanded. (sanguinebio.com)

In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. (bioxcell.com)
Biotinylated anti-CD3ε, anti-T-cell receptor (TCR)αβ, anti-Thy-1.2, and anti-Mac-1 (CD11b) were detected by streptavidin-Cy5 (Jackso. (neuromantics.net)
CTLA-4 is related to the T cell co-stimulatory receptor CD28, and acts to suppress T cell function by competing with CD28 for binding to CD80 and CD86 on antigen presenting cells and recruiting inhibitory molecules into the TCR signaling synapse. (sanguinebio.com)
A second article in the same issue of The Journal of Experimental Medicine by Bulliard et al also explored the role of FcgR engagement on the effects of Ipilimumab as well as an agonistic antibody (DTA-1) targeting the T cell activating receptor GITR (TNFR glucocorticoid-induced TNFR-related protein), which is also expressed on both activated T cells and T REG s. (sanguinebio.com)

There was no consistent decrease in the migration of CD11b + F4/80 + (macrophage) or SSC hi GR-1 + (neutrophil) populations. (biomedcentral.com)
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. (cytekbio.com)

Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (thermofisher.com)
Together, these experiments showed that PD-L1 expression on antigen-presenting cells (APCs) played a role in antitumor T cell responses via PD-1 interactions. (acir.org)

Figure A. FITC conjugated Mouse anti Human CD120a ( MCA1340F ) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control ( MCA928A647 ). (bio-rad-antibodies.com)
Figure B. FITC conjugated Mouse anti Human CD120a ( MCA1340F ) and Alexa Fluor 647 conjugated Mouse anti Human CD11b ( MCA551A647 ). (bio-rad-antibodies.com)
Figure A. FITC conjugated Mouse anti Human CD31 ( MCA1738F ) and PE conjugated Mouse IgG1 isotype control ( MCA928PE ). (bio-rad-antibodies.com)
Figure B. FITC conjugated Mouse anti Human CD31 ( MCA1738F ) and RPE conjugated Mouse anti Human CD11b ( MCA551PE ). (bio-rad-antibodies.com)
Antibodies used for cell surface labeling were purchased from BD PharMingen (San Diego, CA) and included fluorescein isothiocyanate (FITC)-labeled anti-Thy-1.2, anti-Gr-1, and anti-bromodeoxyuridine (BrdU), phycoerythrin-labeled anti-CD4, anti-CD3, and anti-NK1.1. (neuromantics.net)
The samples were then fixed with paraformaldehyde or diluted with PBS followed by incubation with 5 μg/ml PerCP-conjugated anti-CD45, 5 μg/ml FITC-conjugated anti-CD11b, or 80 μM dichlorofluorescein diacetate. (arizona.edu)

cross reactivity of this antibody to other proteins has not been determined. (thermofisher.com)
Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). (thermofisher.com)

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
Multiparameter flow cytometric analysis of CD11b expression on mouse bone-marrow cells. (bdbiosciences.com)
In vitro and in vivo experiments suggest a response of CR3 and CR4 to enable complement-dependent cell cytotoxicity towards antibody-coated cancer cells. (wikipedia.org)
Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. (cytekbio.com)
Flow cytometry surface staining pattern of murine peritoneal fluid cells stained using anti-mouse CD54 (YN1/1.7.4) purified antibody (concentration in sample 4 μg/ml, DAR APC). (exbio.cz)
Separation of murine leukocytes (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells stained using anti-mouse CD54 (YN1/1.7.4) purified antibody (concentration in sample 4 μg/ml, DAR APC). (exbio.cz)
In the context of cancer, appropriately activated DCs can induce anti-tumor immunity by activating innate immune cells and tumor-specific lymphocytes that target cancer cells. (mdpi.com)
Figure 1: Surface staining of PMA-activated human peripheral blood cells with anti-human CD11b activation epitope (CBRM1/5) PE. (abeomics.com)
Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. (bdbiosciences.com)
Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. (thermofisher.com)
Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. (thermofisher.com)
These isotypes were selected to minimise the risk of depleting the T cells upon which such antibodies depend for their mechanism of action. (bmj.com)
Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and 0.25 µg of Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD8a eFluor® 450 (right). (thermofisher.com)
Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Single-cell suspensions from spleens were seeded in a capture antibody pre-coated MultiScreen-IP Filter Plate (five 105 cells per well) and incubated with full medium with or without stimulation of SPSYVYHQF peptidic epitope (ten g/mL, PEPTIDE 2.0, USA). (nicotinic-receptor.com)
First discovered by Ralph Steinman and Zanvil A. Cohn in the 1970s, named for their tree-like branches or "dendrites", these cells patrol our bodies, ingest pathogens, process them, and present fragments (antigens) to T-cells, thus initiating a targeted immune response [2]. (origene.com)
Conventional dendritic cells (cDCs), also known as myeloid dendritic cells or classical dendritic cells, are a type of antigen-presenting cell (APC), cDCs originate from hematopoietic stem cells, specifically from the common myeloid progenitor in the bone marrow. (origene.com)
cDC1: These cells are very effective at presenting antigens to CD8+ T cells, a type of cytotoxic T cell that can kill infected or cancerous cells. (origene.com)
cDC2: These cells are specialized in presenting antigens to CD4+ T cells, a type of helper T cell that helps coordinate the immune response. (origene.com)
Gene expression signatures within memory B cells suggest qualitative differences in the antibody responses in aged patients with severe disease. (medrxiv.org)
Innate lymphoid cells (ILCs) lack antigen receptors but are equipped with receptors for cytokines, hormones, and paracrine signals enabling them to sense their environment and respond quickly by producing factors that influence parenchymal cells and other resident immune cells. (elifesciences.org)
Thus, the mechanism of action of Ipilimumab has been presumed to involve releasing anti-tumor effector T cells from CTLA-4-inhibition and/or limiting T REG activity in the tumor and therefore resulting in an increase in the ratio of effector T cells/ T REG s within the tumor. (sanguinebio.com)
Ipilimumab functions to increase the ratio of effector T cells to T REGS in the tumor microenvironment and has been shown to require binding to both types of T cells for maximal anti-tumor effectiveness. (sanguinebio.com)
Even though GITR-activation in effector T cells promotes activities including cytokine production and proliferation, the agonistic properties of this antibody alone were not effective in the absence of activating FcgR engagement. (sanguinebio.com)
Consistent with in vitro observations, in mice with PD-L1-deficient MC38 colorectal tumors where only host cells were the source of PD-L1, antibody blockade of both PD-1/PD-L1 and PD-L1/B7-1 interactions reduced tumor growth, while blocking only PD-L1/B7-1 interactions slightly accelerated tumor growth due to an increased availability of PD-L1. (acir.org)

Most of these immunomodulatory antibodies are of IgG isotypes that have low, or no, binding to the Fc gamma receptors (FcγRs) that trigger cell-mediated cytotoxic effector functions such as antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP). (bmj.com)
Activating receptors contain cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAM) and activate the FcgR-expressing cell to mediate functions including antibody-dependant cell mediated cytotoxicity (ADCC) and phagocytosis of the antibody-labeled target cell. (sanguinebio.com)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. (angioproteomie.com)
The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. (bioxcell.com)

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. (biolegend.com)
Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. (thermofisher.com)
Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer Staining Set (cat. (thermofisher.com)
Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)

The following product was used in this experiment: FOXP3 Monoclonal Antibody (PCH101), eFluor™ 660, eBioscience™ from Thermo Fisher Scientific, catalog # 50-4776-42, RRID AB_10597604. (thermofisher.com)
The following product was used in this experiment: CD8a Monoclonal Antibody (RPA-T8), eFluor™ 450, eBioscience™ from Thermo Fisher Scientific, catalog # 48-0088-42, RRID AB_1272062. (thermofisher.com)

1-5 In mice, DCs bearing the myeloid marker CD11b (Mac-1), but lacking CD8α, derive efficiently from myeloid precursors and represent the major DC subset in the periphery. (ashpublications.org)

1997. The Leukocyte Antigen FactsBook Academic Press. (elabscience.com)
The purpose of this study was to determine if labeling leukocytes with a monoclonal antibody specific to leukocyte common antigen (CD45) was superior to labeling with LDS-751 and to determine the effect of sample fixation on a mouse whole blood preparation for flow cytometry. (arizona.edu)
We conclude that labeling leukocytes with monoclonal antibody CD45 in a mouse whole blood preparation is preferable, as it provides improved measurement of leukocyte indices compared to LDS-751. (arizona.edu)
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions. (biolegend.com)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
The antibody was purified by affinity chromatography. (angioproteomie.com)
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)

8 , 11 - 14 Numerous stimuli can drive the activation of the complement system, including apoptotic debris, pathogens, and antibody-antigen complexes, in addition to ischemia-reperfusion injuries associated with organ transplantation. (jrheum.org)

Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. (bdbiosciences.com)

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. (biolegend.com)

Thus differential affinities of IgG subclasses to functionally different Fcg receptors are thought to mediate the variation in clinical effectiveness of different antibodies targeting the same antigen. (sanguinebio.com)
Their response involves antigen capture, enhancing their MHC-II presentation receptors and production of chemokines and/or cytokines at the site of inflammation [9]. (fortunejournals.com)

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)

The isotype control suggested for use with this antibody is Purified Mouse IgG2a κ Isotype Control (Cat. (bdbiosciences.com)
For optimal indirect immunohistochemical staining, this antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Rat Anti-Mouse IgG2a (Cat. (bdbiosciences.com)

We found that samples labeled with LDS-751 demonstrated decreased fluorescence intensity for granulocyte CD11b expression and ROS production compared to samples labeled with anti-CD45. (arizona.edu)
In addition, sample fixation decreased mean fluorescence intensity in samples labeled with either LDS-751 or anti-CD45. (arizona.edu)

Single-cell suspensions from tumors were incubated with antibody against CD16/ CD32 (Invitrogen, 14161-86, 1:100), followed by incubation with LIVE/DEAD FixableAuthor Manuscript Author Manuscript Author Manuscript Author ManuscriptBiomaterials. (nicotinic-receptor.com)

Figure A. RPE conjugated Mouse anti Human CD14 ( MCA1568PE ) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control ( MCA928A647 ). (bio-rad-antibodies.com)

6 , 7 Murine DCs with the reciprocal CD8α + CD11b − phenotype, although present at various levels in all lymphoid organs, represent the prototype of the DC population resident in the postnatal thymus. (ashpublications.org)

This study concluded that a major mechanism of action for both antibodies involved engagement of activating FcgRs leading to ADCC-mediated T REG depletion from the tumor. (sanguinebio.com)
Thus, even for functionally different (antagonistic versus agonistic) immunotherapeutic antibodies targeting these same T cell populations, FcgR-mediated ADCC of T REG s appears to be a critical mechanism for anti-tumor effects. (sanguinebio.com)

However, the tumor microenvironment (TME) imposes different mechanisms that facilitate the impairment of DC functions, such as inefficient antigen presentation or polarization into immunosuppressive DCs. (mdpi.com)
Pre-clinical evidence suggests that MDSC suppress anti-tumor immune responses. (iospress.com)
Tumor antigen-specific DC and T cell activation. (nicotinic-receptor.com)
Anti-tumor vaccination. (nicotinic-receptor.com)
Treating tumor-bearing PD-L1 ΔDC mice with anti-PD-L1 antibodies that blocked either only the PD-L1/B7-1 interactions or both the PD-L1/B7-1 interactions and PD-L1/PD-1 interactions did not further enhance tumor control. (acir.org)

The antibody is also reported to be cross-reactive for Rhesus macaque CD11b. (cytekbio.com)

Notably, this new paradigm mechanistically emphasizes the immunomodulatory and anti-inflammatory activities of rexinoids. (aacrjournals.org)
Antibodies targeting T-cell inhibitory pathways, such as CTLA-4 and PD-1/PD-L1, are emerging as an important class of cancer therapeutics, and a next generation of immunomodulatory therapies targeting alternative inhibitory (e.g. (bmj.com)
Here we review the biology of the FcγRs and IgG isotypes in both humans and mice, detail the potential roles that FcγR interactions can play in the activity of monoclonal antibodies in general, and of immunomodulatory antibodies in particular, and discuss how preclinical studies on these interactions might be best interpreted and translated to a human setting. (bmj.com)

The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. (biolegend.com)
TotalSeq™-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions . (biolegend.com)

Figure B. RPE conjugated Mouse anti Human CD14 ( MCA1568PE ) and Alexa Fluor 647 conjugated Mouse anti Human CD11b ( MCA551A647 ). (bio-rad-antibodies.com)

Antibodies of the IgG sub-class are bi-functional molecules, possessing a F(ab) domain, variable in sequence and responsible for the binding of antigen, and an Fc domain, constant in sequence and responsible for mediating a range of antibody effector functions [ 1 ]. (bmj.com)

This antibody may be used for immunohistochemical staining of acetone-fixed, frozen and zinc-fixed, paraffin sections. (bdbiosciences.com)

In mice, MDSC can be identified by species-specific cell surface markers (CD11b + Gr-1 + ) and may be further classified as monocytic (M-MDSC, Ly-6C hi ) or granulocytic (G-MDSC, Ly-6G + ) based on additional cell surface markers [ 6 ]. (iospress.com)

For those who are ineligible for cisplatin-based regimens or experience progression of disease, in 2016-2017 the United States Food and Drug Administration approved five monoclonal antibodies that achieve immune checkpoint blockade by targeting the programmed cell death protein-1/programmed death-ligand 1 (PD-1/PD-L1) pathway. (iospress.com)

After confirming that binding in cis between PD-L1 and B7-1 reduced PD-1 signaling, but not CD28 signaling, the researchers created anti-PD-L1 antibodies that selectively inhibited interactions between PD-L1 and PD-1, PD-L1 and B7-1, or both. (acir.org)

Like the J43 antibody the RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. (bioxcell.com)
A frozen section from rat spleen was stained with Mouse Anti-Rat CD11b/c (Cat.550299). (bdbiosciences.com)

Such biological therapeutic targeting is characterized by lowering autoimmune inflammation or enhancing anti-cancer vaccination effects. (wikipedia.org)
Its anti-inflammatory effect mediation further provides support for its therapeutic promise in animal models of vascular injury. (wikipedia.org)

The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. (exbio.cz)

Durvalumab is a human IgG1-K monoclonal antibody that targets programmed death ligand 1 and is approved by the U.S. Food and Drug Administration for locally advanced or metastatic urothelial cancer and locally advanced, unresectable stage 3 non-small cell lung cancer. (bmj.com)
therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. (bdbiosciences.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
The antigen is extracted from the cell in an appropriate lysis buffer, and antibodies are added to the lysate to allow formation of the immune complex. (neuromantics.net)

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