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  • strains
  • Following initial reconstruction from genome annotation and the literature, we iteratively refined the model by comparing its predictions with the results of large-scale experiments: (1) high-throughput growth phenotypes of the wild-type strain on 190 distinct environments, (2) genome-wide gene essentialities from a knockout mutant library, and (3) large-scale growth phenotypes of all mutant strains on 8 minimal media. (biomedcentral.com)
  • enzyme
  • Further classification into mono- and dioxygenases in based on whether the enzyme causes transfer of just one atom to the substrate (S) (eq. 1) or whether the whole molecule is incorporated into a single substrate (eq. 2a) or is shared by a pair of substrates (S, S′) (eq. 2b) ( 1, 2 ) 1 . (springer.com)
  • The enzyme, studied from phytopathogenic bacteria such as Pseudomonas savastanoi , is involved in a pathway for the production of (indol-3-yl)acetate (IAA), the main auxin hormone in plants. (qmul.ac.uk)
  • metabolic
  • Metabolic engineering of Escherichia coli for improving L: -3,4-dihydroxyphenylalanine (L: -DOPA) synthesis from glucose Journal of Industrial Microbiology & Biotechnology, 38, 1845-1852. (unam.mx)
  • gene
  • 3. The method of claim 2, wherein the second pest pathogenicity gene is a fungus pathogenicity gene, whereby the plant is resistant to a second fungus expressing at least one gene having substantial homology to the second antisense sequence. (patentsencyclopedia.com)
  • 8. The method of claim 1, wherein the first pest pathogenicity gene is from a Phytophthora. (patentsencyclopedia.com)
  • 9. The method of claim 1, wherein the first pest pathogenicity gene is from Phytophthora sojae and wherein the second pathogenicity gene is from Phakospora pachyrhizi. (patentsencyclopedia.com)
  • The predictions of the final version of the model, which included three rounds of refinements, are consistent with the experimental results for (1) 91% of the wild-type growth phenotypes, (2) 94% of the gene essentiality results, and (3) 94% of the mutant growth phenotypes. (biomedcentral.com)
  • The expression cassette of claim 1, wherein the functional equivalent of the at least one transcription regulating nucleotide sequence is obtained or obtainable from plant genomic DNA from a gene encoding a polypeptide which has at least 70% amino acid sequence identity to a polypeptide selected from the group described by SEQ ID NO: 18, 23, 28, 33, 39, and 44, respectively. (patentsencyclopedia.com)
  • metabolites
  • The PrrF sRNAs promote the production of 2-akyl-4(1 H )-quinolone metabolites (AQs) that mediate a range of biological activities, including quorum sensing and polymicrobial interactions. (asm.org)
  • wherein
  • 2. The method of claim 1, wherein the first fungus is a fungus selected from the group consisting of Fusarium solani, Sclerotinia sclerotiorum, Phialophora gregata, Phakospora pachyrhizi, Phytophthora sojae, and Peronospora berteroae. (patentsencyclopedia.com)
  • 6. The method of claim 1, wherein the first fungus can cause a fungal disease selected from the group consisting of brown stem rot, stem and root rot, white mold disease, sudden death syndrome, Asian rust, and downy mildew. (patentsencyclopedia.com)
  • 4. The expression cassette of claim 1, wherein expression of the at least one nucleic acid sequence results in expression of a protein, or expression of an antisense RNA, a sense RNA, or a double-stranded RNA. (patentsencyclopedia.com)
  • 5. The expression cassette of claim 1, wherein expression of the at least one nucleic acid sequence confers to the plant an agronomically valuable trait. (patentsencyclopedia.com)
  • microbial
  • Biosynthesis of catechol melanin from glycerol employing metabolically engineered Escherichia coli Microbial Cell Factories, 15, 161. (unam.mx)
  • iii) Lignin is a complex organic biopolymer mainly present in plants in close association with carbohydrates like cellulose and hemicellulose, its main activity is to provide plants with structural stability, impermeability and resistance towards microbial attack [ 3 ]. (ijbs.com)
  • expression
  • 7. A transgenic host cell or non-human organism comprising the expression cassette of claim 1 or a vector comprising said expression cassette. (patentsencyclopedia.com)
  • 8. A transgenic plant comprising the expression cassette of claim 1, or a vector comprising said expression cassette, or a transgenic cell comprising said expression cassette or said vector. (patentsencyclopedia.com)
  • glucose
  • Plant cells are protected with three layers known as middle lamella, primary and secondary cell walls which are made up of carbohydrates (cellulose, hemicellulose and pectin) and lignin [ 1 ] [Figure 1 ] i) Cellulose is a plant polysaccharide and highly abundant organic polymer on the earth, composed of numerous glucose units linked in β (1→4) linkages, occurring in both crystalline and amorphous forms. (ijbs.com)
  • production
  • Production of Cinnamic and p-Hydroxycinnamic Acids in Engineered Microbes Frontiers in Bioengineering and Biotechnology, 3, 116. (unam.mx)
  • mixture
  • ii) Hemicellulose is a composite compound with a mixture of pentoses, hexoses and sugar acids [ 3 - 6 ]. (ijbs.com)
  • plant
  • In: Plant Science, 95 (2). (iisc.ernet.in)
  • In: Plant Cell, Tissue and Organ Culture, 23 (1). (iisc.ernet.in)
  • Cellulose is present in plant cell walls, stems, straw, stalks and other woody portion of the plant, it alone constitutes to 40-50% of dry weight of plant material [ 2 ]. (ijbs.com)