• The crystal structure is in agreement with the underlying NMR data, but our structure reveals a well-defined set of contacts and also reveals the locations and roles of water molecules at the protein-DNA interface. (rcsb.org)
  • Using buried water molecules to explore the energy landscape of proteins. (iucr.org)
  • Unfortunately, the lack of an evident druggability of this protein, since it has no defined binding or active sites, makes the quest for VDAC interacting molecules a difficult tale. (frontiersin.org)
  • VDACs are the most abundant pore-forming proteins of the MOM and, differently from other structurally similar proteins such as Tom40 or Sam50, they serve as unspecific channels allowing the exchanges of molecules up to a molecular weight of 1,500 Da. (frontiersin.org)
  • Beyond the metabolic functions, the peculiar position of VDACs, at the interface between cytosol and mitochondria, makes porins the mitochondrial docking site for several cytosolic proteins, including molecules involved in the regulation of cell life and death. (frontiersin.org)
  • des(1-6)antennapedia homeodomain: comparison of the NMR solution structure and the DNA-binding affinity with the intact Antennapedia homeodomain. (isni.org)
  • The repressor was affinity captured from D. citri aqueous extracts using biotinylated holin promoter DNA immobilized on magnetic beads and subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein database interrogation was used to identify a small DNA-binding protein encoded by a gene carried by Wolbachia strain wDi, a resident endosymbiont of D. citri as the repressor. (asm.org)
  • Mutations that change the affinity of a small autoactivation element for EXD protein result in corresponding changes in the element's embryonic activity. (embopress.org)
  • In the present work, we report that the deletion of the homeodomain N-terminal arm (first nine residues) of the homeodomain-leucine zipper protein Hahb-4 dramatically affects its DNA-binding affinity, causing a 70-fold increase in dissociation constant. (mysciencework.com)
  • However, the Antennapedia N-terminal arm is not able to confer increased binding affinity to monomers of Hahb-4 lacking the leucine zipper motif, indicating that the inefficient binding of monomers must be due to structural differences in other parts of the molecule. (mysciencework.com)
  • The construction of proteins with modifications at residues 5 to 7 of the homeodomain suggests strongly that positively charged amino acids at these positions play essential roles in determining the DNA-binding affinity. (mysciencework.com)
  • The in vitro -translated Wolbachia repressor protein was able to penetrate L . crescens cells, bind to " Ca . Liberibacter asiaticus" promoter DNA, and partially suppress holin promoter-driven β-glucuronidase (GUS) activity, indicating potential involvement of an additional interacting partner(s) or posttranslational modification(s) for complete suppression. (asm.org)
  • The K88R protein had greatly reduced binding to a TAATCC element and did not specifically bind any other TAATNN motif. (asm.org)
  • Plant homeodomain-leucine zipper proteins, unlike most animal homeodomains, bind DNA efficiently only as dimers. (mysciencework.com)
  • Past structural studies have focused on the binding of monomeric homeodomains to DNA, but two recent structures have revealed how homeodomains bind DNA as multimers. (embl.de)
  • Mutations in the gene for the antennapedia homeodomain protein are associated with the conversion of antenna to leg or leg to antenna DROSOPHILA . (rightdiagnosis.com)
  • Axenfeld-Rieger syndrome is an autosomal-dominant disorder caused by mutations in the PITX2 homeodomain protein. (asm.org)
  • Both of the mutated residues are fully exposed to solvent, and there is no reason to believe that either of these mutations alters the structure of the homeodomain (1). (jci.org)
  • The possibility that the two mutations Asn188Lys and Tyr191Cys alter the DNA binding of the otherwise structurally intact NKX2.5 protein has important implications. (jci.org)
  • To reconstruct the functional evolution of Msx and inform the study of human mutations, we analyzed the phylogeny and synteny of 46 metazoan Msx proteins and tracked the duplication, diversification and loss of conserved motifs. (biomedcentral.com)
  • However, the effect of mutations at positions 6 and 7 can be counteracted by introducing a stretch of positively charged residues at positions 1 to 3 of the homeodomain. (mysciencework.com)
  • Chan S, Popperl H, Krumlauf R, Mann R. An extradenticle-induced conformational change in a HOX protein overcomes an inhibitory function of the conserved hexapeptide motif. (labome.org)
  • A subfamily of HELIX-TURN-HELIX DNA-binding proteins that contain a variable length loop adjacent to the HTH motif. (umassmed.edu)
  • The GnRH regulatory region contains four conserved homeodomain binding sites (ATTA) that are essential for basal promoter activity and cell-specific expression of the GnRH gene. (pubmedcentralcanada.ca)
  • This study was performed to investigate the transduction of a full-length superoxide dismutase (SOD) protein fused to transactivator of transcription (Tat) into human chondrocytes, and to determine the regulatory function of transduced Tat-SOD in the inflammatory cytokine induced catabolic pathway. (biomedcentral.com)
  • Cross-regulatory protein-protein interactions between Hox and Pax transcription factors. (unibas.ch)
  • In this extensively revised second edition, the authors delve into the latest discoveries, incorporating new coverage of comparative genomics, molecular evolution of regulatory proteins and elements, and microevolution of animal development. (b-ok.org)
  • Pax proteins, characterized by the presence of a paired domain, play key regulatory roles during development. (pnas.org)
  • The HD-ZIPIII/ZPR regulatory module would serve not only to dampen the effect of fluctuations in HD-ZIPIII protein levels but more importantly would provide a potential point of regulation (control over the ratio of inactive heterodimers to active homodimers) that could be influenced by other components of the pathway governing leaf polarity. (plantcell.org)
  • Repressor Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Insertion of exogenous ubiquitin into rat brain mitochondria in the presence of ATP and the ATPregenerating system (creatine phosphate/creatine phosphokinase) results in the increase in: sensitivity of mitochondrial monoamine oxidases (MAO) A and B to inhibition by mechanism based inhibitor and incorporation of [ 3 H]-pargyline, which was especially notable in the fraction obtained by immunoprecipitation of mitochondrial proteins with anti-ubiquitin antiserum and protein A Sepharose. (chemweb.com)
  • Insertion of ubiquitin into mitochondria did not influence molecular masses of [ 3 H]-pargyline labeled proteins. (chemweb.com)
  • Shokolenko, I., M. Alexeyev, S. LeDoux, and G. Wilson, TAT-mediated protein transduction and targeted delivery of fusion proteins into mitochondria of breast cancer cells. (freepatentsonline.com)
  • Biochemical and animal model studies of inherited neurodegenerative diseases have revealed that mutant proteins of these diseases are associated with mitochondria. (springer.com)
  • Schmidt, M. Alexander 2017-02-01 00:00:00 Bacterial pathogens have developed intriguing virulence mechanisms, including several sophisticated nanomachines, for injecting effector proteins to manipulate host immune signaling pathways for their own benefit. (deepdyve.com)
  • The oomycete potato late blight pathogen, Phytophthora infestans, and the apicomplexan malaria parasite Plasmodium falciparum translocate effector proteins inside host cells, presumably to the benefit of the pathogen or parasite. (elsevier.com)
  • Proteins containing a POU region consist of a homeodomain and a separate, structurally homologous POU domain that contains two helix-turn-helix motifs and also binds DNA. (blogspot.com)
  • The 2.5 A resolution structure of a cocrystal containing the paired domain from the Drosophila paired (prd) protein and a 15 bp site shows structurally independent N-terminal and C-terminal subdomains. (embl.de)
  • We have exploited this cytoplasmic recognition to report on replacement of the RxLR-EER of Avr3a with the equivalent sequences from the intracellular effectors ATR1NdWsB and ATR13 from the related oomycete pathogen, Hyaloperotiospora parasitica, and the host targeting signal from the Pl. falciparum virulence protein PfHRPII. (elsevier.com)
  • Furthermore, there is an isoform of the Pax-6 protein (Pax-6 5a) that contains, in the middle of the recognition helix of the PAI domain, an 11-residue insertion that inactivates DNA binding to sequences normally bound by the Pax-6 PD ( 6 ). (pnas.org)
  • Hydration and DNA recognition by homeodomains. (iucr.org)
  • Similarities and differences at the corresponding positions within the N-terminal arms are conserved in the vertebrate Abdominal-B-like HOM proteins, which play critical roles in limb specifications as well as in regional specification along the anterior-posterior axis. (nih.gov)
  • Homeodomain proteins play key roles in development and gene regulation in eukaryotes. (embl.de)
  • 1990 ) Molecular genetic manipulation of the red flour beetle: Genome organization and cloning of a ribosomal protein gene. (biologists.org)
  • However, endogenous PDX-1 is usually detected as a protein with molecular mass of 46 kDa, likely due to posttranslational modifications such as phosphorylation and sumoylation. (atlasgeneticsoncology.org)
  • During my student years from 1957-1962, NMR spectroscopy was just being introduced as an analytical tool in chemistry, molecular biology was not yet established as an independent discipline, and the initial three-dimensional protein crystal structures were just emerging. (angelfire.com)
  • 10. Scott, M.P., A.J. Weiner,T.I. Hazelrigg, B.A. Polisky, V. Pirrotta, F. Scalenghe, and T.C. Kaufman (1983) The molecular organization of the Antennapedia Locus of Drosophila. (stanford.edu)
  • Three-dimensional structure of proteins determined by molecular dynamics with interproton distance restraints. (iucr.org)
  • Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release. (harvard.edu)
  • This graph shows the total number of publications written about "Repressor Proteins" by people in Harvard Catalyst Profiles by year, and whether "Repressor Proteins" was a major or minor topic of these publication. (harvard.edu)
  • Below are the most recent publications written about "Repressor Proteins" by people in Profiles. (harvard.edu)
  • The same Hox protein can act as a repressor at one gene and an activator at another. (hitchhikersgui.de)
  • One of the principal differences between HTH motifs in these different proteins arises from the stereo-chemical requirement for glycine in the turn which is needed to avoid steric interference of the beta-carbon with the main chain: for cro and repressor proteins the glycine appears to be mandatory, while for many of the homeotic and other DNA-binding proteins the requirement is relaxed. (embl.de)
  • In the present study, 11 recombinant fusion proteins were analyzed to characterize sequences and conditions that affect protein uptake and/or activity and to develop more active cell-permeant enzymes. (biomedcentral.com)
  • The attachment of a virion particle with the surface of an individual cell is based upon the interaction between cell surface receptors and the particles composing the capsid or proteins on the surface of the virion. (jyi.org)
  • Duplicated domains allow pleiotropically expressed proteins to evolve new functions without disrupting existing interaction networks. (biomedcentral.com)
  • Within each A/P domain, a different HOX protein assigns positional identities which are eventually realized in diverse morphological structures on the segmented A/P axis of the Drosophila embryo ( McGinnis and Krumlauf, 1992 ). (embopress.org)
  • Comparison of protein structures determined by NMR in solution and by X-ray diffraction in single-crystals. (iucr.org)
  • Since recombination provides a stable and unambiguous record of protein uptake, the enzyme may also be used for quantitative studies of cis - and trans -acting factors that influence the delivery of proteins into cells. (biomedcentral.com)
  • Our results thus implicate a second class of chromatin-associated proteins in mediating dCBP function and imply that dCBP might be involved in the regulation of higher-order chromatin structure. (labome.org)
  • Eyg also contains another DNA-binding domain, a Prd-class homeodomain (HD), whose palindromic binding site is similar to other Prd-class HDs. (pnas.org)
  • The Arabidopsis thaliana REVOLUTA (REV) protein is a member of the class III homeodomain-leucine zipper (HD-ZIPIII) proteins. (plantcell.org)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing HOX domain in the selected taxonomic class. (embl.de)