• Please can you give us a short summary of the recent reports article, 'Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi' ? (biotechniques.com)
  • Microbiota modification is a new challenge to limit disease transmission but it still needs advanced knowledges on bacterial community in Anopheles , especially in wild and infected specimens from diverse origin and species. (frontiersin.org)
  • Bacterial culture and 16S rRNA gene-PCR associated to Temporal Temperature Gradient Electrophoresis (TTGE) were applied to explore the bacterial diversity in the abdomen of 100 wild specimens (eight Anopheles species) collected in the Binh Phuoc Province, Vietnam. (frontiersin.org)
  • Anopheles associated-bacteria appeared specimen-dependent rather than mosquitoe species- or group-dependent. (frontiersin.org)
  • Simultaneous microarray-based transcription analysis of 4987 Anopheles stephensi midgut and Plasmodium berghei infection stage specific cDNAs was done at seven successive time points: 6, 20 and 40h, and 4, 8, 14 and 20 days after ingestion of malaria infected blood. (unboundmedicine.com)
  • The study reveals the molecular components of several Anopheles processes relating to blood digestion, midgut expansion and response to Plasmodium-infected blood such as digestive enzymes, transporters, cytoskeletal and structural components and stress and immune responsive factors. (unboundmedicine.com)
  • Temporal correlation between transcription profiles of both organisms identifies putative gene clusters of interacting processes, such as Plasmodium invasion of the midgut epithelium, Anopheles immune responses to Plasmodium infection, and apoptosis and expulsion of invaded midgut cells from the epithelium. (unboundmedicine.com)
  • The naturally acquired microbiota of Anopheles can influence vector's susceptibility to Plasmodium and its capacity to transmit them. (frontiersin.org)
  • Occurrence of the leucine-to-phenylalanine knockdown resistance (kdr) mutation in Anopheles arabiensis populations in Tanzania, detected by a simplified high-throughput SSOP-ELISA method. (www.gov.uk)
  • Application of the SSOP-ELISA method to 1,620 field-collected Anopheles arabiensis from Tanzania identified the West African leucine-phenylalanine kdr mutation in two heterozygous individuals, indicating the potential for resistance development that requires close monitoring. (www.gov.uk)
  • She is currently working on the development and optimization of a gene drive system based on CRISPR/Cas9 technology for population modification of the malaria African vector Anopheles gambiae to spread beneficial genes quickly through mosquito populations. (biotechniques.com)
  • Results: Literature searches yielded a total of 4,084 susceptibility data points for 1,505 populations, and 2,097 resistance mechanisms data points for 1,000 populations of Anopheles spp. (vectormap.info)
  • Methods: A systematic search of published, peer-reviewed literature was performed and Anopheles insecticide susceptibility and resistance mechanisms data were extracted and added to a database after a two-level verification process. (vectormap.info)
  • Conclusions: The increasing pyrethroid and DDT resistance in Anopheles in the Afrotropical region is alarming. (vectormap.info)
  • IR Mapper is a useful tool for investigating temporal and spatial trends in Anopheles resistance to support the pragmatic use of insecticidal interventions. (vectormap.info)
  • Therefore, new and innovative approaches to control Anopheles vectors for reducing malaria transmission on a more durable and safer manner for human health are, hence, needed. (frontiersin.org)
  • However, Proteobacteria represented by 57 genera was the most diversified phylum in Anopheles microbiota. (frontiersin.org)
  • The high overall of Anopheles -associated bacteria is confirmed with, to our knowledge, 51 genera described for the first time in Anopheles microbiota. (frontiersin.org)
  • Consequently, the core microbiota share by Anopheles from Binh Phuoc was very narrow, suggesting that Anopheles microbiota was greatly influenced by local environments. (frontiersin.org)
  • The culicine and Anopheles clades of mosquitoes diverged between 120 million years ago and 150 million years ago. (wikipedia.org)
  • Like all mosquitoes, Anopheles are attracted to your body heat and the carbon dioxide you exhale, which they can detect 75 feet away - so you might try holding your breath. (giantmicrobes.com)
  • Anopheles mosquitoes are most active just before dawn and at dusk. (giantmicrobes.com)
  • Anopheles mosquitoes kill about 440,000 people each year because of malaria. (wn.com)
  • We tested Anopheles gambiae mosquitoes from Côte d'Ivoire for resistance and screened a subset The Study for target site mutations. (cdc.gov)
  • Mosquitoes of the genus Anopheles are well known as primary vectors of malaria. (eurekalert.org)
  • In addition, the researchers noted, Anopheles mosquitoes tend to take multiple blood meals between egg-laying events, and this bite frequency increases their capacity to transmit viruses. (eurekalert.org)
  • Despite the fact that Anopheles mosquitoes are widely dispersed worldwide, they currently are neglected as potential vectors of arboviruses," Rasgon said. (eurekalert.org)
  • We report the distribution of mosquitoes of the maculipennis complex in two distinct areas of the Czech Republic (Bohemia and South Moravia) and in one locality of neighbouring Slovakia with emphasis on the detection of the newly described cryptic species Anopheles daciae (Linton, Nicolescu & Harbach, 2004). (springer.com)
  • Bates M (1940) The nomenclature and taxonomic status of the mosquitoes of the Anopheles maculipennis complex. (springer.com)
  • Danabalan R, Monaghan MT, Ponsonby DJ, Linton YM (2013) Occurrence and host preferences of Anopheles maculipennis group mosquitoes in England and Wales. (springer.com)
  • mosquitoes were trapped in Anopheles bancroftii an area of 6 km2, out of 50 km2 covered by the survey. (cdc.gov)
  • Metabolomic and ecdysteroid variations in Anopheles gambiae s.l. mosquitoes exposed to the stressful conditions of the dry season in Burkina Faso, West Africa," Physiological and Biochemical Zoology , vol. 87, no. 3, pp. 486-497, 2014. (hindawi.com)
  • Exposure of Anopheles gambiae mosquitoes to Plasmodium infection enhances the ability of their immune system to respond to subsequent infections. (sigmaaldrich.com)
  • Using a dual-choice olfactometer, the role of L-lactic acid was investigated in relation to host-seeking and selection by female Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) mosquitoes in a Y-tube bioassay. (nih.gov)
  • Anopheles quadrimaculatus mosquitoes are primarily seen in eastern North America. (ufl.edu)
  • not determine which sibling species of the complex are present in those provinces, Mosquitoes of the Anopheles maculipennis and since then no further studies have been complex have been incriminated as some of carried out to determine current distribu- the main malaria vectors in Europe and the tion. (who.int)
  • Salivary gland homogenates from adult female Anopheles albimanus mosquitoes relaxed aortic rings preconstricted with noradrenaline (NA). (biologists.org)
  • It is suggested that adult female Anopheles albimanus mosquitoes contain a salivary heme peroxidase that functions during blood finding and blood feeding by destroying hemostatically active biogenic amines released by the vertebrate host during tissue destruction. (biologists.org)
  • Collections of Anopheles gambiae Giles complex, A. funestus Giles group and other mosquitoes were made by spray-catch from twelve catching stations indoors and by hand-catch from pit shelters at two catching stations at Segera, Tanzania, between January 1970 and June 1971. (cambridge.org)
  • Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae , including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. (g3journal.org)
  • To investigate the genetic basis of olfactory modulation in mosquitoes, we assayed changes in CO 2 detection from receptor gene expression through physiological function to behaviour, associated with the onset of host seeking in the malaria vector, Anopheles gambiae . (biologists.org)
  • Whilst physical and chemical cues used by Culex and Aedes vector mosquitoes for selecting an oviposition site have been extensively studied, those for Anopheles remain poorly explored. (diva-portal.org)
  • Anopheles gambiae mosquitoes were collected from four sites across Benin and identified to species/molecular form. (biomedcentral.com)
  • The development of insecticide resistance in anopheles mosquitoes is a major threat for malaria vector control. (biomedcentral.com)
  • Human Malaria is transmitted by mosquitoes of the genus Anopheles . (biomedcentral.com)
  • In several contexts, the estimation of the fitness of Anopheles mosquitoes is required. (biomedcentral.com)
  • Anopheles mosquitoes are responsible for transmitting human malaria parasites that cause an estimated 200 million cases and more than 600 thousand deaths each year. (unige.ch)
  • Two articles published on November 27th in the journal Science describe detailed genomic comparisons of these mosquitoes and the deadliest of them all, Anopheles gambiae . (unige.ch)
  • Malaria parasites are transmitted to humans by only a few dozen of the many hundreds of species of Anopheles mosquitoes, and of these, only a handful are highly efficient disease-vectors. (unige.ch)
  • In insects, including Anopheles mosquitoes, Dscam (Down syndrome cell adhesion molecule) appears to be involved in phagocytosis of pathogens, and shows pathogen-specific splice-form expression between divergent pathogen (or parasite) types (e.g. between bacteria and Plasmodium or between Plasmodium berghei and Plasmodium falciparum ). (biomedcentral.com)
  • Significant increases in Anopheles gambiae Dscam (AgDscam) receptor diversity were observed in parasite-exposed mosquitoes, but only weak evidence that AgDscam diversity rises further upon exposure to mixed genotype parasite infections was found. (biomedcentral.com)
  • Leukocytes in a Plasmodium falciparum-infected blood meal reduce transmission of malaria to Anopheles mosquitoes. (asm.org)
  • Effect of predation on Anopheles larvae by five sympatic insect families in coastal Kenya. (thefreelibrary.com)
  • Studying predation pressure on Anopheles mosquito larvae is made more complicated by having at least two distinct guilds of predators (12). (thefreelibrary.com)
  • The Anopheles mosquito larvae, filter feeding at the surface of the water, must contend with potential attacks from both surface and submerged predators. (thefreelibrary.com)
  • Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s. (mdpi.com)
  • In this study, we have investigated if ATP-binding cassette (ABC) transporters, a major component of the defensome machinery, are involved in defence against the insecticide permethrin, in susceptible larvae of the malaria vector Anopheles gambiae sensu stricto. (mdpi.com)
  • The plant species studied were selected on the basis of criteria (Table 1), such as lack of information on activity against Anopheles and Culex larvae, botanical families (Euphorbiaceae, Verbenaceae, and Meliaceae) from which number of larvicidal plant were reported and large distribution in West Africa. (thefreelibrary.com)
  • Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago. (wikipedia.org)
  • ABSTRACT A diagnostic polymerase chain reaction (PCR) assay using species-specific primers and direct sequencing was used to identify members of the Anopheles maculipennis complex in the north-west and central regions of the Islamic Republic of Iran. (who.int)
  • The system of subgenera originated with the work of Christophers, who in 1915 described three subgenera: Anopheles (widely distributed), Myzomyia (later renamed Cellia) (Old World) and Nyssorhynchus (Neotropical). (wikipedia.org)
  • The number of species currently recognised within the subgenera is given here in parentheses: Anopheles (206 species), Baimaia (1), Cellia (216), Kerteszia (12), Lophopodomyia (6), Nyssorhynchus (34) and Stethomyia (5). (wikipedia.org)
  • The larger subgenera (Anopheles, Cellia and Nyssorhynchus) have been subdivided into sections and series which in turn have been divided into groups and subgroups. (wikipedia.org)
  • Anopheles albertoi Unti e Anopheles arthuri Unti são retiradas da sinonímia com Anopheles strodei Root, e uma forma morfologicamente distinta, adiante designada Anopheles CP, do Complexo Strodei de Anopheles (Nyssorhynchus) é caracterizada. (usp.br)
  • Anopheles albertoi Unti and Anopheles arthuri Unti are revived from the synonymy with Anopheles strodei Root, and a distinct morphological form (herein designated Anopheles CP Form) from the Strodei complex of Anopheles (Nyssorhynchus) is characterized. (usp.br)
  • Subgenera Anopheles , Cellia and Nyssorhynchus are subdivided hierarchically into nested informal groups of morphologically similar species that are believed to represent monophyletic lineages based on morphological similarity. (cambridge.org)
  • Abdulla-Khan , R. , Coetzee , M. & Hunt , R.H. ( 1998 ) Description of Anopheles (Cellia) seretsei sp. (cambridge.org)
  • The analyses included 12 species within Anopheles and 4 within the Myzorhynchus Series of the subgenus Anopheles , 8 within Neocellia, 6 within Myzomyia, 3 within Paramyzomyia, and 1 within the Pyretophorus Series of the subgenus Cellia . (go.jp)
  • The congruent tree topologies of both molecular markers strongly supported monophyly of subgenera Anopheles and Cellia . (go.jp)
  • http://www.softberry.com/berry.phtml?topic=gfind Method description: A new parameter set for gene prediction Anopheles gambiae is developed for FGENESH program. (bio.net)
  • She is currently working on the development and optimization of a gene drive system based on CRISPR/Cas9 technology for population modification of the malaria African vector Anopheles gambiae to spread beneficial genes quickly through mosquito populations. (biotechniques.com)
  • Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae ," BMC Genomics , vol. 6, article 5, 2005. (hindawi.com)
  • The ancestral STAT gene (AgSTAT-A) appears to have duplicated in the mosquito Anopheles gambiae, giving rise to a second intronless STAT gene (AgSTAT-B), which we show regulates AgSTAT-A expression in adult females. (nih.gov)
  • Bacterial culture and 16S rRNA gene-PCR associated to Temporal Temperature Gradient Electrophoresis (TTGE) were applied to explore the bacterial diversity in the abdomen of 100 wild specimens (eight Anopheles species) collected in the Binh Phuoc Province, Vietnam. (frontiersin.org)
  • OBP3 gene cDNA ORF clone, Anopheles gambiae str. (genscript.com)
  • Their origin and the existence of Anopheles -specific bacterial taxa are discussed. (frontiersin.org)
  • Populations of Anopheles (Kerteszia) were sampled fortnightly over a one-year period (August 1991 to July 1992) at Ribeira Valley, S. Paulo State, Brazil. (scielo.br)
  • the major African malaria vector Anopheles gambiae is notoriously difficult to manipulate genetically. (g3journal.org)
  • Here we take a systems genetics approach, analyzing two inversion systems implicated in climatic adaption by Anopheles gambiae . (pnas.org)
  • The Genetics of Inviability and Male Sterility in Hybrids Between Anopheles gambiae and An. (genetics.org)
  • Comparative studies on sibling species of the Anopheles gambiae Giles complex (Dipt. (cambridge.org)
  • In a study carried out by Service (5) on natural mortalities and predation of immature stages of Anopheles gambiae Giles, mortality was observed to be higher in the rice pools than in the small pools and ponds. (thefreelibrary.com)
  • However, the results, reported today (Nov. 7) in PLoS Neglected Tropical Diseases , demonstrated that all four Anopheles species were competent laboratory vectors of the virus. (eurekalert.org)
  • The Anopheles genome, at 230-284 million base pairs (Mbp), is comparable in size to that of Drosophila, but considerably smaller than those found in other culicine genomes (528 Mbp-1.9 Gbp). (wikipedia.org)
  • The Anopheles gambiae genome: an update. (genscript.com)
  • Much of our understanding of many such processes derives from the sequencing of the Anopheles gambiae genome in 2002, which has since facilitated many large-scale functional studies that have offered numerous insights into how this mosquito became highly specialized in order to live amongst and feed upon humans. (unige.ch)
  • Until now, the lack of such genomic resources for other Anopheles limited comparisons to small-scale studies of individual genes with no genome-wide data to investigate key attributes that impact the mosquito's ability to transmit parasites. (unige.ch)
  • An association of brain tumor incidence and malaria suggests the Anopheles might transmit a virus or other agent that could cause a brain tumor. (wn.com)
  • The capacity of the two North American species of Anopheles to transmit Mayaro is particularly relevant to the United States, because the estimated geographic distribution of these species covers the entire country," Rasgon said. (eurekalert.org)
  • Variation in the ability of different Anopheles species to transmit malaria - known as "vectorial capacity" - is determined by many factors, including feeding and breeding preferences, as well as their immune responses to infections. (unige.ch)
  • Insecticide resistance in Anopheles sacharovi Favre in southern Turkey. (who.int)
  • Prevalence of insecticide resistance in Anopheles multiple insecticide-resistance mechanisms ( 4 ). (cdc.gov)
  • A major threat to further reductions in malaria incidents is the emergence and spread of insecticide resistance (IR) in Anopheles ( An . (springer.com)
  • We examine the extent and impact of recent introgression of a strongly selected insecticide-resistance mutation ( Vgsc-1014F ) located within one of two exceptionally large genomic islands of divergence separating the Anopheles gambiae species pair. (nature.com)
  • In the Anopheles minimus complex, 2 sibling species (A and C) are generally accepted. (bioone.org)
  • Here, the morphology of the spermatheca in the saltwater-tolerant mosquito Anopheles aquasalis Curry was investigated for the first time using a combination of light, confocal, and scanning and transmission electron microscopy. (bioone.org)
  • Anopheles subpictus is a complex of four isomorphic sibling species A, B, C and D and is recognized as a primary vector of malaria, a disease of great socio-economic importance, in Australasian Zone, Celebes, Portuguese Timor and South East Asia and a secondary vector in Sri Lanka. (malariaworld.org)
  • Phylogenetic trees constructed on the basis of ITS2 sequences could accurately categorize all of the series according to the classical taxonomy but could not distinguish Pyretophorus ( Anopheles subpictus ) from Paramyzomyia Series. (go.jp)
  • Blazquez J, de Zulueta J (1980) The disappearance of Anopheles labranchiae from Spain. (springer.com)
  • We performed an analysis of the electrophysiological responses of olfactory neurons in the antennae of the female malaria mosquito Anopheles gambiae s.s. and investigated the effect of blood feeding on responsiveness. (sigmaaldrich.com)
  • Molecular assay were used to determine distribution of Anopheles gambiae sub-species. (rti.org)
  • Djadid D, Gholizadeh S, Tafsiri E, Romi R, Gordeev M, Zakeri S (2007) Molecular identification of Palearctic members of Anopheles maculipennis in northern Iran. (springer.com)
  • In the taxonomy published by Harbach et al in 2016, it was shown that three species of Bironella: confusa, gracilis, and hollandi are phylogenetically similar Anopheles kyondawensis than other Bironella species. (wikipedia.org)
  • Anopheles quadrimaculatus Say is historically the most important vector of malaria in the eastern United States. (ufl.edu)
  • Adult female common malaria mosquito, Anopheles quadrimaculatus Say. (ufl.edu)
  • Known distribution of Anopheles quadrimaculatus Say. (ufl.edu)
  • Anopheles quadrimaculatus has dark scales on the wings with patches of scales forming four darker spots on the wing (Carpenter and LaCasse 1955). (ufl.edu)
  • Egg of the common malaria mosquito, Anopheles quadrimaculatus Say. (ufl.edu)
  • Anopheles quadrimaculatus will feed on mammals including humans and domestic and wild animals (Carpenter and LaCasse 1955). (ufl.edu)
  • In depth annotation of the Anopheles gambiae mosquito midgut transcriptome," BMC Genomics , vol. 15, article no. 636, 2014. (hindawi.com)
  • We show that a peroxidase, secreted by the Anopheles gambiae midgut, and dual oxidase form a dityrosine network that decreases gut permeability to immune elicitors. (sciencemag.org)
  • Malaria transmission has been eradicated from southern European Russia and Siberia but the main mosquito vector Anopheles messeae still flourishes. (europa.eu)
  • Anopheles farauti is a complex of seven species distributed in the Moluccas (Indonesia) and extend eastward through Papua New Guinea (the Admiralty Islands and the Bismarck Archipelago), the Solomon Islands, the New Hebrides (Vanuatu) and Australia. (vectorbase.org)
  • However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae . (jove.com)
  • Phylogenetic analysis of Anopheles mosquito species introduced in New Caledonia. (cdc.gov)
  • The phylogenetic relationships of Anopheles spp. (go.jp)