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PhospholipidsAnionsPhosphatidylserinesCardiolipinsPhosphatidylglycerolsPhosphatidylcholinesLiposomesMembrane LipidsPhosphatidylethanolaminesbeta 2-Glycoprotein ILipid BilayersSaposinsAnnexin A5Phosphatidic AcidsCell MembranePhosphatidylinositolsPhospholipid Transfer ProteinsMembranes, ArtificialUnithiolSphingolipid Activator ProteinsProtein BindingMonoglyceridesKinetics1,2-DipalmitoylphosphatidylcholineSpectrometry, FluorescenceStatic ElectricityPhosphatidylinositol 4,5-DiphosphateLipidsCalorimetry, Differential ScanningMolecular Sequence DataSqualene MonooxygenaseAmino Acid SequencePhospholipid EthersMicellesGlycerophospholipidsPhospholipases ABinding SitesProteolipidsHydrogen-Ion ConcentrationPerchloratesFluorescent DyesCattleMolecular ConformationThromboplastinAntibodies, AntiphospholipidProthrombinCholesterolGlycoproteinsMembranesDetergentsBlood CoagulationFatty AcidsModels, MolecularLupus Coagulation InhibitorSurface PropertiesEscherichia coliTemperatureMembrane ProteinsAntiphospholipid SyndromeAnilino NaphthalenesulfonatesFactor VaGlucosylceramidaseIntracellular MembranesModels, ChemicalOsmolar ConcentrationPhosphatidylinositol PhosphatesProtein ConformationIonsProtein Structure, TertiaryHydrolysisFactor XaCalciumWaterProtein Structure, SecondaryChromatography, Thin LayerAntigens, CD36PhospholipasesBinding, CompetitivePeptidesBiological TransportCircular DichroismRecombinant ProteinsSurface Plasmon ResonanceStructure-Activity RelationshipEnzyme ActivationSubstrate SpecificitySpectrometry, Mass, Electrospray IonizationPhospholipases A2DimyristoylphosphatidylcholineSphingomyelinsBacterial ProteinsMicroscopy, Atomic ForceSodium ChlorideSurface-Active AgentsOxidation-ReductionEscherichia coli ProteinsMicroscopy, FluorescenceX-Ray DiffractionMutationAdenosine Triphosphatases
MembranesCardiolipinMembrane phospholipidsPhosphatidylserineBinds to the anionicPhosphatidicBilayersVitroVesiclesAcidicElectrostatic interactionsAntibodyCurvatureBiosynthesisCompositionsInteractionHomeostasisPhosphatidylinositolLysineConcludeBoundSpeciesMediatesComplexesTissueMicrobialProteinProteinsAmountsExteriorPercentageCellSurfaceActivationPresenceStrainResultsHuman
Membranes9
  • In vitro beta 2GPI binds to anionic phospholipids and inhibits the prothrombinase activity of procoagulant membranes. (jci.org)
  • All-atom simulations of 4E10, PGZL1, 10E8 and LN01 docked onto HIV-like membranes consistently form phospholipid complexes at key complementarity-determining region loop sites, solidifying that stable and specific lipid interactions anchor bnAbs to membrane surfaces. (elifesciences.org)
  • Recently, the researchers have discovered that the spatial organization of phospholipids at the curved regions of bacterial cytoplasmic membranes has an important physiological role in bacteria, including the regulation and positioning of the universal enzyme RecA. (wisc.edu)
  • Blood coagulation involves a series of zymogen acti-vation reactions that are mainly performed by enzyma- tic complexes consisting of a serine protease, a protein co-factor and membranes containing anionic phospholipids (1). (scielo.br)
  • Phosphatidylethanolamine (PE) is frequently the main lipid component of microbial membranes and the second most abundant phospholipid in mammals, comprising as much as 45% of brain lipids. (matreya.com)
  • It appears that a primary role for PE, in bacterial membranes at least, is simply to dilute the high negative charge density of the anionic phospholipids. (matreya.com)
  • Szeto-Schiller (SS) peptides comprise a class of amphipathic tetrapeptides that have demonstrated efficacy in treating a wide array of mitochondrial disorders, and are believed to target mitochondrial membranes due to their enrichment in the anionic phospholipid cardiolipin (CL). (biorxiv.org)
  • These proteins typically act by interacting with anionic phospholipid membranes, disrupting their structure and forming pores which lead to cell lysis, inactivation or depolarization of the cell. (scoiltrad.com)
  • The proteins triggering an abnormal accumulation of membrane structures inside the cells (or membrane proliferation) share two major common features: 1) they promote the formation of highly curved membrane domains and 2) they lead to an enrichment in anionic, cone-shaped phospholipids (cardiolipin or phosphatidic acid) in the newly formed membranes. (preprints.org)
Cardiolipin3
  • Oxidation of major classes of phospholipids - phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), and cardiolipin (CL) - was determined by electro-spray ionization mass spectrometry as well as by fluorescence HPLC/Amplex Red assay. (cdc.gov)
  • This strain produced triglycerides (TG) as well as four common phospholipids: phosphatidylethanolamine (PE), phosphatidylglycerol (PG), cardiolipin (CL) and lysocardiolipin (LCL). (nature.com)
  • Figure 1 shows that RecA binds to the anionic phospholipids phosphatidylglycerol (PG) and cardiolipin (CL) with an apparent Kd of 7.0 and 2.4 M, respectively. (wisc.edu)
Membrane phospholipids3
  • Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids. (addgene.org)
  • One hypothesis postulates a defect in cellular apoptosis, which exposes membrane phospholipids to the binding of various plasma proteins, such as beta-2 glycoprotein I. Once bound, a phospholipid-protein complex is formed and a neoepitope is uncovered, which subsequently becomes the target of autoantibodies. (medscape.com)
  • membrane phospholipids, and other anionic phospholipid-containing moieties. (bvsalud.org)
Phosphatidylserine1
  • In normal blood vessels, they face the interior of the cell.Dr. Thorpe's previous research has shown that anionic phospholipids, particularly one called phosphatidylserine, are already flipped inside-out on tumour endothelial cells. (myvmc.com)
Binds to the anionic1
  • aCL binds to the anionic phospholipid on the platelet membrane and to the b2-GPI free flowing in the serum. (medscape.com)
Phosphatidic2
  • Both full-length SEIPIN and its lumenal domain can bind anionic phospholipids including phosphatidic acid. (princeton.edu)
  • Phosphatidylinositols are acidic (anionic) phospholipids that consist of a phosphatidic acid backbone, linked via the phosphate group to inositol (hexahydroxycyclohexane). (hmdb.ca)
Bilayers3
  • The effect of molecular structure on ensemble structure and dynamics of phospholipid bilayers has been investigated. (birmingham.ac.uk)
  • Horswell, Sarah L. / Effect of electric field on structure and dynamics of bilayers formed from anionic phospholipids . (birmingham.ac.uk)
  • Manuel M, Martins J . Partitioning of 1-pyrenesulfonate into zwitterionic and mixed zwitterionic/anionic fluid phospholipid bilayers . (ualg.pt)
Vitro1
  • Engineered mutations to the 10E8 paratope at light-chain solvent-exposed residues that add positive charge or hydrophobic sidechains proved to increase antibody association to anionic phospholipid vesicles in vitro and also boosted neutralization potency 27 . (elifesciences.org)
Vesicles3
  • In preliminary studies it was shown that LFABP released the fluorescent fatty acid 11(5-dimethylaminonapthalenesulphonylamino) undecanoic acid (DAUDA) in the presence of anionic phospholipid vesicles under conditions that indicated a direct interaction between the LFABP and the anionic phospholipid interface. (soton.ac.uk)
  • The ligand release that was observed with anionic vesicles was sensitive to the ionic strength of the assay conditions and the anionic charge density of the phospholipid at the interface, indicating that non-specific electrostatic interactions play an important role in the process. (soton.ac.uk)
  • The nature of this interaction involves both electrostatic and non-polar interactions as maximal release of LFABP from phospholipid vesicles with recovery of ligand binding cannot be achieved with high salt and requires the presence of a non-anionic detergent. (soton.ac.uk)
Acidic1
  • In contrast, the two acidic phospholipids, DPPS and DPPA, caused a dose- dependent increase in both lysis and C5b-7 uptake. (psu.edu)
Electrostatic interactions1
  • SANS data for the protein/lipid complexes were obtained with contrast-matched bicelles under various phospholipid compositions to probe the role of electrostatic interactions. (cea.fr)
Antibody1
  • The flipping is likely due to stress conditions present in the tumour micro-environment, and radiation increases the number of exposed phospholipids," said Dr. Thorpe.Once they induced more flipping with radiation, the researchers administered bavituximab, a monoclonal antibody that homes in on tumour vessels by selectively binding to the inside out phospholipids. (myvmc.com)
Curvature1
  • Membrane curvature induced by proximity of anionic phospholipids can initiate endocytosis. (buffalo.edu)
Biosynthesis3
  • Phospholipid biosynthesis is crucial for the successful intracellular survival and replication of the parasites, as the phospholipids have important roles in the biogenesis of membrane organelles, signal transduction and other cellular processes. (hu-berlin.de)
  • Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. (preprints.org)
  • Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. (preprints.org)
Compositions1
  • The phospholipid compositions of the invention are well tolerated by human tissue making them suitable for use as preservative and disinfectant components in the preparation of personal care, household cleaning and like products which exhibit enhanced antimicrobial and antifungal characteristics. (justia.com)
Interaction2
  • The most likely explanation for ligand release is that interaction of LFABF with an anionic membrane interface induces a rapid conformational change, reducing the affinity of DAUDA for the protein. (soton.ac.uk)
  • The IRG tested the hypothesis that the localization and function of the large, polar fraction of RecA in E. coli depends on its interaction with anionic phospholipids that accumulate at the large, negatively curved region of the cytoplasmic membrane. (wisc.edu)
Homeostasis2
  • Our results suggest that SEIPIN forms a scaffold that helps maintain phospholipid homeostasis and surface tension of the ER. (princeton.edu)
  • Lipidomic profiling of the PIS mutant demonstrated selective reduction of certain PtdIns and PtdThr species, whereas selected PtdGro, PtdSer and BMP species were increased, which suggested a tight inter-regulation and homeostasis of anionic phospholipids to maintain the membrane integrity. (hu-berlin.de)
Phosphatidylinositol2
  • The major anionic head groups can be quantified in single runs with practical detection limits of about 100 pmol, and the D3 isoforms of phosphatidylinositol phosphate (PIP) and PIP 2 are detected as shoulder peaks. (elsevierpure.com)
  • Phosphatidylinositol is especially abundant in brain tissue, where it can amount to 10% of the phospholipids, but it is present in all tissues and cell types. (hmdb.ca)
Lysine1
  • Lysine residues in the α-helical region of adipocyte and heart FABP had previously been identified as important for interactions between these proteins and the phospholipid interface. (soton.ac.uk)
Conclude2
  • We conclude that the presence of anionic lipids on the exterior face of the membrane increases C5b-7 uptake and subsequent hemolysis. (psu.edu)
  • We conclude that anionic phospholipid-mediated signaling may participate in phagocytosis induced macrophage apoptosis. (cdc.gov)
Bound1
  • When bound to anionic bicelles, large modifications of the protein threedimensional structure were detected, as revealed by a significant increase of the protein gyration radius from 42 5 1 to 60 5 4 A . R1-3/anionic bicelle complexes were further analyzed by coarse-grained molecular dynamics simulations. (cea.fr)
Species3
  • To study PIP 2 levels of cells without radiolabeling, we have developed a new method to quantify anionic phospholipid species. (elsevierpure.com)
  • We hypothesized that triggering of phagocytosis and activation of macrophages - including NADPH oxidase generated derived reactive oxygen species - mediates selective oxidation of phospholipids participating in the execution of apoptotic program. (cdc.gov)
  • Moreover, oxidation of the same species of anionic phospholpipids was detected in lungs of mice exposed to single walled carbon nanotube s via pharengeal inhalation. (cdc.gov)
Mediates1
  • It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. (thermofisher.com)
Complexes1
  • We reveal the formation of amorphous complexes via an en masse pathway and their relaxation into virions via a synchronous pathway. (cea.fr)
Tissue1
  • These synthetic phospholipids are suggested as exhibiting an outstanding combination of surfactant characteristics as well as being well tolerated by human tissue, i.e., they exhibit exceptionally low ocular irritation and oral toxicity. (justia.com)
Microbial1
  • While these known phospholipids have been found useful as surfactants in a variety of personal care, household cleaning and the like products, such products also require the incorporation of antimicrobial preservatives to inhibit microbial spoilage and increase the shelf life. (justia.com)
Protein4
  • In HeLa, Hek 293 and COS cells, as well as intact heart, PIP 2 amounts to 0.5 to 1.5% of total anionic phospholipid (10 to 30 μmol/liter cell water or 0.15 to 0.45 nmol/mg protein). (elsevierpure.com)
  • It has been reported that antiphospholipid autoantibodies do not recognize phospholipid alone, but rather the plasma protein beta 2-glycoprotein I (beta 2GPI), or a beta 2GPI-phospholipid complex. (jci.org)
  • Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. (nih.gov)
  • Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. (nih.gov)
Proteins1
  • In this study we assessed the role of anionic lipids in the binding of the terminal complement proteins to the membrane and the efficiency of subsequent hemolysis. (psu.edu)
Amounts1
  • Its activity depends on the presence of small amounts of anionic phospholipids such as phosphatidyl serine. (tcdb.org)
Exterior1
  • In the setting of initial injury, the anionic surface is exposed to the exterior. (medscape.com)
Percentage1
  • The binding signals white blood cells from the immune system to attack and destroy the vessels feeding the tumour.In their study of mice, the researchers found that radiation increased the percentage of phospholipids that flip inside out from 4 percent to 26 percent. (myvmc.com)
Cell1
Surface1
  • The stoichiometric relationship between anionic phospholipid and LFABP suggests that the LFABP coats the surface of the phosphilipid vesicle. (soton.ac.uk)
Activation2
  • The second minor site may correspond to the secondary anionic phospholipid site required for channel activation. (wustl.edu)
  • Deposition of this aCL immune complex causes additional exposure of anionic phospholipids and platelet activation by unidentified signal transducing mechanisms. (medscape.com)
Presence1
  • R1-3 free in solution was compared to its structure adopted in the presence of phospholipid-based bicelles. (cea.fr)
Strain1
  • These studies provide the IRG with insight into how membrane strain is connected to the organization of phospholipids and the biological function of the resulting material. (wisc.edu)
Results1
  • The results of these studies suggested that α-helix II, in particular, residue K31 of LFABP are involved in interactions with the anionic phospholipid interface. (soton.ac.uk)
Human1