Anion exchange protein 1. Medical search. Frequent questions

Bio-Rad Laboratories, Inc has launched EconoFit Low-Pressure Prepacked Chromatography Column Packs to support resin screening experiments in the development of protein purification workflows. (labmate-online.com)
Ion exchange (IEX) chromatography is used to separate proteins based on their net charge at a particular pH. (hydrocarbononline.com)
Anion-exchange chromatography was performed in a denaturing and reducing imidazole-Hel buffer at pH 7 as per Thompson et al. (edu.au)
Pellicular anion-exchange chromatography applied to RNAi assays for monitoring strand stoichiometry and RNA stability. (chromatographytoday.com)
In order to characterize the chicken HSL enzyme, it was purified 3500 fold from a chicken adipose tissue homogenate using pH 5.2 precipitation and anion-exchange chromatography. (lu.se)
3H]CFT binding sites were separated into three protein fractions by anion-exchange chromatography. (aspetjournals.org)
Size-exclusion chromatography indicated [3H]CFT bound to a single protein in each fraction (apparent molecular weight, 170 kDa). (aspetjournals.org)
A 35 kDa protein was purified to 1.5-fold with total yield of 13.02 % by applying anion exchange and gel filtration chromatography. (preprints.org)
FBP was purified from osmotic-shock fluid using anion-exchange fast protein liquid chromatography (FPLC). (microbiologyresearch.org)
Compatible purification steps include Protein A chromatography, virus filtration, anion and cation exchange chromatography, mixed-mode, hydrophobic-interaction and size- exclusion chromatography. (cygnustechnologies.com)

Specific point mutations in human anion exchanger 1 (AE1) convert this electroneutral anion exchanger into a monovalent cation conductance. (tcdb.org)
Anion exchanger 1 (AE1), also known as band 3 or SLC4A1, plays a key role in the removal of carbon dioxide from tissues by facilitating the exchange of chloride and bicarbonate across the plasma membrane of erythrocytes. (rcsb.org)
Here we report the crystal structure of the band 3 anion exchanger domain (AE1(CTD)) at 3.5 angstroms. (rcsb.org)

The new packs include mixed-mode, cation, and anion exchange resin columns, as well as a pack designed for His-tag protein purification. (labmate-online.com)
A New High-resolution Strong Cation-exchange 1 mm i.d. (chromatographyonline.com)
ProSwift strong cation-exchange (SCX) column is a polymethacrylate-based monolithic column with sulfonate functionality. (chromatographyonline.com)
Earlier, reversed-phase, weak and strong anion-exchange and weak cation-exchange monolithic columns were introduced in a 1 mm i.d. format for protein separations. (chromatographyonline.com)
Reversed phase, pi-pi and cation exchange interactions. (thomassci.com)
HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification. (sigmaaldrich.com)
The small particle size (34 μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. (sigmaaldrich.com)
The strong anion exchange (SAX) and weak cation exchange (WCX) chips were used for analysis. (bmj.com)

Importantly, both hOAT1 and rat renal organic anion transporter 1 (rROAT1) mediated saturable, probenecid-sensitive uptake of cidofovir, adefovir, and other nucleoside phosphonate antivirals. (aspetjournals.org)
Pombrio JM, Giangreco A, Li L, Wempe MF, Anders MW, Sweet DH, Pritchard JB, Ballatori N. Mercapturic acids (N-acetylcysteine S-conjugates) as endogenous substrates for the renal organic anion transporter-1. (ucdenver.edu)

The TSKgel DNA-NPR weak anion exchange column is designed to analyze nucleic acids. (selectscience.net)

Field method for the determination of hexavalent chromium by ultrasonication and strong anion-exchange solid-phase extraction. (cdc.gov)

Remove impurities in biopharmaceutical process streams such as DNA, host cell proteins (HCP) and viruses with our all-synthetic, single use 3M™ Emphaze AEX Hybrid Purifier. (3m.com)
The scale-down model used to compare the performance of the different AEX products/formats is summarized in Table I. We evaluated five commonly used AEX products: three resins and two membrane adsorbers for dynamic binding capacity of DNA and protein using bovine serum albumin (BSA) to mimic common contaminants, such as host cell proteins, removed during a FT step. (biopharminternational.com)

A high-performance anion exchange resin performs well compared with membranes. (biopharminternational.com)
The exact function of aSyn remains unclear, but it is known to be associated with vesicles and membranes, and to have an impact on important cellular functions such as intracellular trafficking and protein degradation systems, leading to cellular pathologies that can be readily studied in cell-based models. (biomedcentral.com)
Under physiological conditions, aSyn is considered to be a pre-synaptic protein [ 37 ] that associates with vesicles and membranes [ 11 ]. (biomedcentral.com)

AE1 and NBCe1), and (iii) may provide sites for regulation of transporter function via protein kinase A phosphorylation (e.g. (tcdb.org)
Comparing this structure with a substrate-bound structure of the uracil transporter UraA in an inward-facing conformation allowed us to identify the anion-binding position in the AE1(CTD), and to propose a possible transport mechanism that could explain why selected mutations lead to disease. (rcsb.org)
A cDNA clone was isolated from a human renal library and designated human organic anion transporter 1 (hOAT1). (aspetjournals.org)

Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
HSL from chicken adipose tissue exhibits remarkable activation upon phosphorylation with cAMP-dependent protein kinase (cAMP-PK) compared to HSL from rat and human adipose tissue. (lu.se)

Endo- β -1,4-xylanases cleave the backbone of xylan (i.e., the β -1,4- d -xylose chain) in the middle, generating substrates for the β -xylosidase which cleaves off d -xylose from the non-reducing ends of the xylan backbone. (biomedcentral.com)

Choose analytical grade AG 1-X8 Resin for strong anion exchange. (bio-rad.com)

Particularly, linear retention data for various proteins were obtained on two structurally different anion exchange stationary-phase materials in the presence of three salts with different counterions. (edu.in)
Both cidofovir and adefovir are nucleoside phosphonate analogs, a class of novel antivirals structurally related to natural nucleotides (Fig. 1 ). (aspetjournals.org)

Interpretation of the models revealed that particular trends for proteins and salts could be captured using QSRR techniques. (edu.in)

This was mediated via membrane Cl(-)/HCO(3)(-) exchange (the AE1 gene product), irrespective of whether recovery was from an intracellular acid or base load, and with no evident contribution from other transporters such as Na(+)/H(+) exchange. (ox.ac.uk)

He underwent fluid resuscitation and investigations showed the following results: serum sodium 128 mmol/L, chloride 76 mmol/L, potassium 3.3 mmol/L, bicarbonate 28 mmol/L, anion gap 15.6 mmol/L, urea 35 mg/dL, creatinine 1.3 mg/dL, calcium 10.5 mg/dL, phosphorus 7.6mg/dL and magnesium 2.3 mg/dL. (who.int)

Organic Anion Transport Protein 1" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
It mediates the coupled exchange of alpha-ketoglutarate with organic ions such as P-AMINOHIPPURIC ACID. (ucdenver.edu)
This graph shows the total number of publications written about "Organic Anion Transport Protein 1" by people in this website by year, and whether "Organic Anion Transport Protein 1" was a major or minor topic of these publications. (ucdenver.edu)
Below are the most recent publications written about "Organic Anion Transport Protein 1" by people in Profiles. (ucdenver.edu)

Many of the substances under investigation possess the disadvantage of being a large molecular weight proteins that are difficult to manufacture and must be administered by infusion. (justia.com)

TSKgel Butyl-NPR hydrophobic interaction columns for analysis of antibody-drug conjugates (ADCs) and as an orthogonal method to analyze protein aggregation, oxidation and deamidations. (selectscience.net)
These data suggest that the negatively charged glutamate residue may affect the folding and, consequently, the aggregation process of the protein. (biomedcentral.com)
Parkinson's Disease (PD) associated state of neuroinflammation due to the aggregation of aberrant proteins is widely reported. (biorxiv.org)

The monomeric protein (M r 34200 by SDS-PAGE and 37700 by gel-filtration FPLC) bound D-[U -14 C]-fructose stoichiometrically (1.17 nmol nmol FBP -1 ) and with high affinity (K D 0.49 μM) as shown by equilibrium dialysis. (microbiologyresearch.org)

Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)

BLM encodes 1417 amino acids that code for a protein in the nuclear matrix of growing cells, which is a member of the RecQ family of helicases. (medscape.com)

Application of mass spectrometry in the discovery of small molecule inhibitors of DNA repair proteins as potential anticancer drugs," 2nd International Conference on Current Trends in Mass Spectrometry, Chicago, IL, July 20, 2016. (nist.gov)
The objective of this study was to identify new plasma biomarkers in ovarian cancer patients using mass spectrometry (MS) protein profiling and artificial intelligence. (bmj.com)

Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation. (liverpool.ac.uk)

aSyn is a disordered and abundant neuronal protein whose normal function is still elusive. (biomedcentral.com)

Small molecule inhibitors of DNA glycosylases as potential drugs in cancer therapy," 2nd International Conference on Molecular Biology, Nucleic Acids & Molecular Medicine (Keynote Speaker), Philadelphia, PA, September 1, 2017. (nist.gov)

2007). These proteins are important for the regulation of intracellular pH (pH i ) and play crucial roles in the epithelial absorption of HCO 3 - (e.g., in the renal proximal tubule) and secretion of HCO 3 - (e.g., in the pancreatic duct). (tcdb.org)

The animal AE proteins consist of homodimeric complexes of integral membrane proteins that vary in size from about 900 amino acyl residues to about 1250 residues. (tcdb.org)
In solution, compstatin forms a β-turn at residues Gln-5-Gly-8 with the disulfide bridge Cys-2-Cys-12, residues Ile-1-Val-4 and Thr-13 forming a hydrophobic cluster (PCT Pub. (justia.com)
In the plant, arabinans were abundant polysaccharide, which were mainly composed of α- l -arabinofuranosyl residues, and are generally arranged in α-1,5-linked chains with varying numbers of residues substituted with other α- l -arabinofuranoside at the O-2 and/or O-3 position [ 1 ]. (springeropen.com)

Anion exchange protein 3 is a membrane transport protein that in humans is encoded by the SLC4A3 gene. (wikipedia.org)

Our columns deliver run-to-run uniformity to support preparative scale purifications and/or method development for new target proteins, as well as to improve existing workflows. (labmate-online.com)

Protein targeting to glycogen (PTG) is a scaffolding protein that targets protein phosphatase 1α (PP1α) to glycogen, and links it to enzymes involved in glycogen synthesis and degradation. (jci.org)

In the kidney, oxalate is secreted in the proximal tubule via 2 separate carriers involving sodium and chloride exchange. (medscape.com)

Although the C-terminal domain comprises a small percentage of the total protein, this domain in some cases (i) has PSD-95/Discs Large/ZO-1-binding motifs that may be important for protein-protein interactions (e.g. (tcdb.org)

We describe here simple anion-exchange chromatographic approaches to accomplish these separations. (chromatographytoday.com)

1 The column is available in 1 × 50 mm format and is used for high-resolution separation of proteins. (chromatographyonline.com)
The new ProSwift SCX-1S column has a pore structure designed for efficient separation of proteins at elevated linear velocities. (chromatographyonline.com)
Separation of a standard protein mixture at increasing flow rates and constant column volume gradient is shown in Figure 1(a). (chromatographyonline.com)
Figure 1: Separation of proteins using ProSwift SCX-1S, 1 Ã 50 mm column. (chromatographyonline.com)
The ProSwift SCX-1S, 1 mm i.d. column provides high-resolution and cost-effective separation of simple and complex protein mixtures. (chromatographyonline.com)
On-chip sample injection, separation, and detection of seven common anions are successfully demonstrated with a sensitivity of 1 ppm. (caltech.edu)
Gradient separation at a pressure of 450 psi and on-line MS detection of digested cytochrome c protein is successfully performed. (caltech.edu)

The N-terminal A domain differs among the four subtypes of Ca 2+ channel β subunits (β 1 -β 4 ) primarily as the result of two duplications of an ancestral gene containing multiple alternatively spliced exons. (jneurosci.org)
The transactivator Xyr1 (Xylanase regulator 1) is essential for gene expression of most cellulolytic and xylanolytic enzymes [ 10 ]. (biomedcentral.com)
The gene encoding these proteins is FANCM . (medscape.com)

AE3 is functionally similar to the Band 3 Cl−/HCO3− exchange protein but it is expressed primarily in brain neurons and in the heart. (wikipedia.org)

Quantitative structure retention relationship (QSRR) models based on support vector machine feature selection and regression models were developed using the experimental chromatographic data in conjunction with various molecular descriptors computed from protein crystal structure geometries. (edu.in)

We purified SMG1 and SMG1C for biochemical and structural analyses (Supplementary information, Fig. S 1 ). (nature.com)
Their N-terminal hydrophilic domains may interact with cytoskeletal proteins and therefore play a cell structural role. (tcdb.org)
Structural prediction of sugar-binding proteins functional in chemotaxis and transport. (microbiologyresearch.org)

Any process that stops, prevents or reduces the frequency, rate or extent of anion transmembrane transport. (mcw.edu)

Immunoblot analysis of human kidney cortex demonstrated that hOAT1 is an 80- to 90-kilodalton heterogeneous protein modified by abundant N -glycosylation. (aspetjournals.org)
The present work shows that carboxy-SNARF-1, a pH fluorophore, when used with confocal imaging or flow cytometry, reliably reports pH(i) in individual, human RBCs, provided intracellular fluorescence is calibrated using a 'null-point' procedure. (ox.ac.uk)
Here, we aimed to spatially characterise the human Parkinsonian nigro-striatal N- glycome, and related transcriptome/proteome, and its correlation with endoplasmic reticulum stress and unfolded protein response (UPR), providing a comprehensive characterisation of the PD molecular signature. (biorxiv.org)

One clue to this dilemma has emerged from the identification of "targeting" subunits that allow for the compartmentalized activation of the phosphatase, resulting in the specific dephosphorylation of discrete pools of proteins within the cell. (jci.org)
Where the activity (or defect) in a specific protein is known to produce or support a disease state, turnover of the mRNA encoding that specific protein will suppress progression of that disease, or it symptoms. (chromatographytoday.com)
The crude cell extract showed 10.34 U/mL of C12O activity with a specific activity of 2.23 U/mg of protein. (preprints.org)
SELDI mass spectroscopy was highly reproducible in detecting ovarian tumor-specific protein profiles. (bmj.com)
Four specific protein peaks were identified in plasma of women with ovarian cancer, but not in controls, with relative molecular masses of 6190.48, 5147.06, 11522.6, and 11537.7 d. (bmj.com)

Insulin produces the dephosphorylation of only a small subset of proteins at discrete locations, whereas PP1 is ubiquitously expressed and is found in virtually all cellular compartments, suggesting that mechanisms exist for the targeted regulation of PP1 in insulin-responsive cells that selectively permit activation of the enzyme only at these sites. (jci.org)
Conventional methods for determination of EVs include direct detection via enzyme-linked immunosorbent assay and detection of their membrane proteins via western blotting. (exosome-rna.com)
This task is usually achieved by using in particular or surfactants to break start the cellular membrane and release the DNA, as well as a protease enzyme in order to down protein that may be joining to the GENETICS. (icnd.info)
The enzyme, thiocyanate dehydrogenase (TcDH), reveals several features special to the chemistry of biological opper is widely utilized in life systems (1, 2). (docslib.org)

Although mammalian SLC4/AE polypeptides mediate only electroneutral Cl - -anion exchange, trout erythroid AE1 also promotes osmolyte transport and increased anion conductance. (tcdb.org)
It is concluded that fructose is transported into A. radiobacter and related organisms via a periplasmic fructose/mannose-binding-protein-dependent active-transport system, in contrast to the phosphotransferase system used by many other species of bacteria. (microbiologyresearch.org)
Binding- protein-dependent glucose transport by Agrobacterium radiobacter grown in continuous culture. (microbiologyresearch.org)
Binding- protein-dependent sugar transport by Agrobacterium radiobacter and A. tumefaciens grown in continuous culture. (microbiologyresearch.org)
TcDH contains a unique metal site comprising Crated into proteins for the purposes of copper trafficking three copper ions in a configuration that enables two-electron and storage (3, 4) and electron transport and catalysis (5-7). (docslib.org)

They thought that inefficient removal of peroxide might be responsible for the high rates of sister chromatid exchange and chromosomal damage in Bloom syndrome cells. (medscape.com)

Lactobacilli and the commensal bacteria Escherichia coli Nissle and Bacteroides thetaiotaomicron only degraded the F2 compound α-D-Glc p -(1 → 2)-[β-D-Gal p -(1 → 4)-]D-Glc p , constituting around 30% w / w of GL34. (springer.com)
The N-terminal amino acid sequence of FBP (ADTSVCLI-) was similar to that of several sugar-binding proteins from other species of bacteria. (microbiologyresearch.org)

All AE proteins are hypothesized to share a similar topology in the cell membrane. (tcdb.org)
Protopectinases which were consisted of various different enzymes can promote the solubilization of protopectin from the plant cell and can be applied in the protein industry extraction. (springeropen.com)
Exosomes contain various molecular constituents of their cell of origin, including proteins and RNA. (exosome-rna.com)
The ER is the 'port of entry' for proteins destined for the cell surface and beyond. (stanford.edu)
A comparable volume-pH(i) link may also be important in other cell types expressing anion exchangers. (ox.ac.uk)
[ 7 , 8 ] Sister chromatid exchanges are considered a sensitive indicator for cell genome instability, as they are thought to be the outcome of DNA double-strand breaks resulting from homologous recombination repair. (medscape.com)

FineTest offers customers a variety of Cardiac Research products with high specificity, sensitivity and purity, namely Elisa kits, Antibodies and Proteins. (fn-test.com)
[ 1 ] Bloom syndrome has three cardinal features: sun sensitivity, telangiectatic erythema of the face, and stunted growth. (medscape.com)

The identified gained and lost protein peaks in plasma may provide as candidate proteins to be used for the detection or monitoring ovarian cancer. (bmj.com)

temperature: 30 Â°C. Chromatogram (a) 1 = ribonuclease A, 2 = cytochrome C and 3 = lysozyme, 1 mg/mL each, flow rate: 0.1 mL/min, gradient: 1â 95% B in 8.0 min, or 0.2 mL, gradient: 1â 95% B in 4.0 min, or 0.3 mL/min, gradient: 1â 95% B in 2.67 min. (chromatographyonline.com)

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